CN116121179A

CN116121179A - Sperm optimization and detection method, sperm optimization and detection device, electronic equipment and storage medium

Info

Publication number: CN116121179A
Application number: CN202310244206.8A
Authority: CN
Inventors: 滕晓明; 郭毅; 范宇平; 杨昊; 沈艺; 马萌萌; 王羽; 王建峰; 陈思思; 高慧杰; 刘如垚; 闫飞妃
Original assignee: Suzhou Basecare Medical Device Co ltd; Shanghai First Maternity and Infant Hospital
Current assignee: Suzhou Basecare Medical Device Co ltd; Shanghai First Maternity and Infant Hospital
Priority date: 2023-03-14
Filing date: 2023-03-14
Publication date: 2023-05-16
Anticipated expiration: 2043-03-14
Also published as: CN116121179B

Abstract

The invention discloses a sperm optimizing and detecting method, a sperm optimizing and detecting device, electronic equipment and a storage medium. Wherein the sperm preferability and detection method comprises: acquiring original semen, and performing microfluidic treatment on the original semen through a microfluidic chip to obtain primary semen; carrying out liquid flow treatment on the primary semen by a flow cytometer to obtain sperms to be detected; and determining a motion detection result and a morphology detection result of each sperm to be detected to obtain a target detection result. By adopting the technical scheme provided by the embodiment of the invention, the accuracy of the target detection result can be further improved and the sperm optimization quality can be improved on the basis of effectively obtaining the single sperm to be detected with high activity and no damage.

Description

Sperm optimization and detection method, sperm optimization and detection device, electronic equipment and storage medium

Technical Field

The present invention relates to the field of medical application technologies, and in particular, to a sperm optimizing and detecting method, a sperm optimizing and detecting device, an electronic device, and a storage medium.

Background

About 10% -15% of all sterile couples worldwide, about 50% of these cases are related to men, and the incidence of unexplained male infertility is about 15%. Male sperm abnormalities are one of the major causes of natural conception failure, and in recent years, sperm are preferred to gradually enter a critical moment along with the development of assisted reproductive technology.

In the prior art, the common method for optimizing the sperms is an upstream-downstream method, but the limit on the quality of the sperms is higher; density gradient centrifugation, but is prone to physical damage to sperm; the magnetic bead sorting method is expensive; glass fiber filtration, but at a higher cost; flow cytometry, but is prone to causing genetic information mutations in spermatogenesis; deep learning screening methods, but screening data is less accurate. Therefore, sperm are preferably less accurate.

Disclosure of Invention

The invention provides a sperm optimizing and detecting method, a sperm optimizing and detecting device, electronic equipment and a storage medium, and aims to solve the technical problem of poor precision of sperm optimizing.

According to an aspect of the present invention, there is provided a sperm cell preference and detection method, wherein the method comprises:

acquiring original semen, and performing microfluidic treatment on the original semen through a microfluidic chip to obtain primary semen;

carrying out liquid flow treatment on the primary semen by a flow cytometer to obtain sperms to be detected;

and determining a motion detection result and a morphology detection result of each sperm to be detected to obtain a target detection result.

According to another aspect of the present invention, there is provided a sperm cell preference and detection apparatus comprising:

the microfluidic processing module is used for acquiring original semen, and performing microfluidic processing on the original semen through the microfluidic chip to obtain primary semen;

the liquid flow treatment module is used for carrying out liquid flow treatment on the primary semen through a flow cytometer to obtain sperms to be detected;

and the neural network module is used for determining the movement detection result and the morphology detection result of each sperm to be detected to obtain a target detection result.

According to another aspect of the present invention, there is provided an electronic apparatus including:

at least one processor; and

a memory communicatively coupled to the at least one processor; wherein, the liquid crystal display device comprises a liquid crystal display device,

the memory stores a computer program executable by the at least one processor to enable the at least one processor to perform the sperm cell optimization and detection method of any one of the embodiments of the present invention.

According to another aspect of the present invention there is provided a computer readable storage medium storing computer instructions for causing a processor to perform a sperm optimization and detection method according to any of the embodiments of the present invention.

According to the technical scheme, the original semen is obtained, the microfluidic chip is used for performing microfluidic treatment on the original semen to obtain the primary semen, and the primary screening is performed on the sperms in the original semen to remove the sperms with lower vigor; carrying out liquid flow treatment on the primary semen by a flow cytometer to obtain sperms to be detected, and obtaining nondestructive single sperms to be detected; and determining the motion detection result and the morphology detection result of each sperm to be detected to obtain a target detection result, so that the accuracy of the target detection result can be further improved and the sperm optimization quality can be improved on the basis of obtaining the single sperm to be detected with higher activity and no damage.

It should be understood that the description in this section is not intended to identify key or critical features of the embodiments of the invention or to delineate the scope of the invention. Other features of the present invention will become apparent from the description that follows.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a flow chart of a sperm cell optimization and detection method provided in accordance with a first embodiment of the present invention;

FIG. 2 is a schematic diagram of a microfluidic chip embodying an embodiment of the present invention;

FIG. 3 is a scene diagram of a flow cytometer implementing embodiments of the invention;

FIG. 4 is a flow chart of a sperm cell preferred and detection method provided in accordance with a second embodiment of the present invention;

FIG. 5 is a scene graph of a first location, a second location, and a third location implementing an embodiment of the invention;

FIG. 6 is a schematic diagram of a sperm cell preferred and detecting apparatus in accordance with a third embodiment of the present invention;

FIG. 7 is a schematic diagram of the structure of an electronic device implementing the sperm cell optimization and detection method of an embodiment of the present invention.

Detailed Description

In order that those skilled in the art will better understand the present invention, a technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.

It should be noted that the terms "first," "second," and the like in the description and the claims of the present invention and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the invention described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

Example 1

Fig. 1 is a flow chart of a sperm cell preference and detection method according to an embodiment of the present invention, which may be adapted for use in connection with sperm cell screening, which may be performed by a sperm cell preference and detection device which may be implemented in hardware and/or software, which may be deployed in a medical device. As shown in fig. 1, the method includes:

s110, acquiring original semen, and performing microfluidic treatment on the original semen through a microfluidic chip to obtain primary semen.

Wherein the raw semen is understood as raw semen after liquefaction, which has not been treated.

The microfluidic chip may be understood as a chip capable of performing a microfluidic process on the raw semen (refer to fig. 2).

It is to be understood that the microfluidic chip may mimic the female reproductive system such that sperm mimic the act of swimming in the female reproductive system against cervical mucus flow. In the embodiment of the invention, the original semen is subjected to microfluidic treatment by the microfluidic chip, so that the sperms in the semen can be subjected to primary selection of vitality, and the sperms with lower vitality or the sperms without vitality, namely dead sperms, are removed, so as to obtain the sperms with higher vitality.

In the embodiment of the present invention, the microfluidic chip includes a liquid adding chamber 201, a sampling chamber 202, a sample adding chamber 203, and a waste liquid chamber 204, and are sequentially connected through narrow passageways (refer to fig. 2). Specifically, the original semen is obtained through the sample adding chamber, glycoprotein gel liquid is obtained through the liquid adding chamber, the original semen is subjected to flow-tending treatment according to current generated by the flow of the glycoprotein gel liquid, the primary semen in the sample adding chamber flows to the sampling chamber, and in the original semen, waste semen except the primary semen flows to the waste liquid chamber, and the primary semen is obtained through the sampling chamber. In the embodiment of the invention, the original semen is subjected to micro-fluidic treatment by the micro-fluidic chip, so that healthy, active and swimming sperms in the original semen can flow to the sampling chamber almost without damage.

The primary semen can be understood as semen obtained after the microfluidic treatment of the primary semen. In the embodiment of the invention, for example, in the case that the quality of the original semen is high, the total sperm amount in the primary semen may be 10% -20% of the total sperm amount in the original semen; in the case of a lower quality of the primary semen, the total sperm count in the primary semen may be between 0% and 5% of the total sperm count in the primary semen.

And S120, carrying out liquid flow treatment on the primary semen through a flow cytometer to obtain sperms to be detected.

Wherein the flow cytometer may be understood as a device for subjecting the preliminary semen to a liquid flow (refer to fig. 3).

It is to be understood that the primary semen obtained based on the microfluidic treatment is made to enter into a flow cytometer, the flow cytometer can apply a micro pressure to the primary semen, and the sample injection rate of the primary semen can be adjusted by changing the micro pressure, so that the primary semen flows into a portion with a smaller cross-sectional area from a portion with a larger cross-sectional area, so as to obtain a single and nondestructive sperm to be detected.

In the embodiment of the invention, the flow cytometer comprises a sample injection needle 1, a sheath liquid city 2, an inverted magnetic bead 3, a sheath liquid inlet 4, a screw system 5, a primary flow chamber 6, a conical screw channel 7, a secondary flow chamber 8, a transition section 9 and a nozzle 10.

Wherein, sample introduction needle 1: for placing the preliminary semen obtained through the sampling chamber. For example, for placing microfluidic treated semen.

Sheath fluid chamber 2: for containing sheath fluid.

Inverted magnetic beads 3: the inverted magnetic beads rotate around the central axis of the sheath liquid chamber under the action of the electromagnet so as to drive the sheath liquid to rotate.

Sheath fluid inlet 4: the flow cytometer includes 4 evenly arranged sheath fluid inlets.

Screw system 5: the device mainly comprises an electromagnet, and is used for enabling sheath liquid in a sheath liquid chamber to obtain an initial speed and form a vortex structure when the electromagnet drives the inverted magnetic beads to rotate; when sheath liquid is needed to be supplemented, the spiral system is opened, and the sheath liquid smoothly enters the primary flow chamber through conical spiral channels uniformly distributed along the circumferential direction of the sheath liquid chamber under the centrifugal action.

Primary flow chamber 6 and secondary flow chamber 8: the cross section of the secondary flow chamber is smaller than that of the primary flow chamber, so that a laminar flow structure of liquid is formed, and the buffer effect can be achieved, so that the laminar flow of the liquid is more stable. The sheath liquid inlet can increase the outflow of the sheath liquid in unit time, and meanwhile, the sheath liquid is dispersed into a plurality of groups to flow out respectively, so that the influence on the laminar flow of the flow chamber can be reduced.

Conical spiral channel 7: an electromagnetic valve is arranged, and the electromagnetic valve is helpful for controlling the opening and closing of the spiral system, so that the sheath fluid flow from the sheath fluid chamber to the primary flow chamber is accurately controlled. Under the action of the conical spiral channel, the speed of sheath liquid entering the primary flow chamber is higher, and the laminar flow is more stable.

Nozzle 10: for uniform ejection of individual ones of the sperm to be detected.

The sperm to be detected may be understood as sperm to be detected obtained based on the flow cytometer.

In the embodiment of the invention, the primary semen is subjected to liquid flow treatment by the flow cytometer, so that the damage of DNA of the sperm to be detected is avoided, and meanwhile, the energy is effectively saved, and the sperm to be detected is ensured to singly enter the next step for detection without damage.

Optionally, before the target detection result is obtained by determining the motion detection result and the morphology detection result of each sperm to be detected, the method further includes:

and uniformly distributing the sperm to be detected to obtain a single sperm to be detected.

Optionally, uniformly distributing the sperm to be detected through a grid counting plate to obtain single sperm to be detected. The network counting plate can be understood as a device which can uniformly distribute and count the sperms to be detected.

Specifically, in the embodiment of the invention, the sperms to be detected determined based on the flow cytometry are uniformly distributed in each channel of the grid counting plate, and when the number of the sperms to be detected in the channel meets the preset number, the next detection is performed. Wherein the preset number may be 50.

In the embodiment of the invention, in order to save time, the sperms entering the grid counting plate can be classified by a classification algorithm, and other sperms which are not the sperms to be detected can be pushed out of the channel of the grid counting plate by a compression method.

S130, determining a motion detection result and a morphology detection result of each sperm to be detected to obtain a target detection result.

Wherein, the movement detection result can be understood as the detection result of the movement of the sperm to be detected singly. In the embodiment of the present invention, the motion detection result may be preset according to the scene requirement, which is not specifically limited herein. Alternatively, the motion detection result may include forward fast, forward slow, no motion, and the like.

The morphological detection result can be understood as a detection result of the morphology of the sperm to be detected alone. In the embodiment of the present invention, the morphological detection result may be preset according to the scene requirement, which is not specifically limited herein. Alternatively, the morphology detection result may include a normal morphology and an abnormal morphology.

The target detection result may be understood as a detection result determined based on the motion detection result and the morphological detection result. Alternatively, the target detection result may include normal morphological sperm that are fast forward, normal morphological sperm that are slow forward, abnormal morphological sperm that do not move, and so on.

Optionally, the sperm optimizing and detecting method further comprises:

and determining the sperm type of the sperm to be detected according to the target detection result.

Wherein the sperm type is understood to be the usable type of sperm to be detected. Alternatively, the sperm types may include usable sperm and non-usable sperm. Specifically, the sperm type of the normal morphology sperm that is fast forward can be determined as available sperm; the sperm types of normal morphology sperm, non-motile abnormal morphology sperm, and other sperm that were slow forward were determined to be unusable sperm.

Example two

Fig. 4 is a flowchart of a sperm optimizing and detecting method according to a second embodiment of the present invention, where the determining a movement detection result and a morphology detection result of each sperm to be detected in the foregoing embodiment is performed to obtain a target detection result for refinement. As shown in fig. 4, the method includes:

s210, acquiring original semen, and performing microfluidic treatment on the original semen through a microfluidic chip to obtain primary semen.

S220, carrying out liquid flow treatment on the primary semen through a flow cytometer to obtain sperms to be detected.

S230, determining images to be detected in a preset time period for each sperm to be detected, and obtaining a preset number of images to be detected based on the images to be detected.

The preset time period may be understood as a time period corresponding to the image to be detected. In the embodiment of the present invention, the preset time period may be preset according to a scene requirement, which is not specifically limited herein. Alternatively, the preset time period may be 1 second, 2 seconds, 3 seconds, or the like.

The image to be detected may be understood as an image of each of the sperm to be detected on the grid counting plate. Alternatively, the image to be detected may be a 2 second video. Specifically, the device for acquiring the image to be detected may be a high-speed camera.

The preset number can be understood as the number of images to be detected corresponding to the images to be detected in the preset time period. In the embodiment of the present invention, the preset number may be related to parameters of a device for acquiring the image to be detected. Optionally, the preset number of images to be detected corresponding to the images to be detected for 2 seconds may be 50 frames.

The image to be detected may be understood as an image determined based on the image to be detected.

S240, determining a motion detection result corresponding to the sperm to be detected based on the image to be detected, and determining a morphological detection result corresponding to the sperm to be detected based on the image to be detected.

In the embodiment of the invention, the steps of determining the movement detection result corresponding to the sperm to be detected and determining the morphological detection result corresponding to the sperm to be detected are not sequential.

Optionally, the determining, based on the image to be detected, a morphological detection result corresponding to the sperm to be detected includes:

determining a segmentation detection image corresponding to the image to be detected, determining a first position, a second position and a third position corresponding to the sperm to be detected based on the segmentation detection image, and determining a first value corresponding to the first position, a second value corresponding to the second position and a third value corresponding to the third position;

and determining a morphological detection result corresponding to the sperm to be detected according to the first value, a first preset interval corresponding to the first value, the second value, a second preset interval corresponding to the second value, the third value and a third preset interval corresponding to the third value.

The segmentation detection image may be understood as a segmentation image corresponding to the image to be detected. Specifically, the image to be detected is processed through an example segmentation algorithm, and the sperm to be detected is segmented from the background to obtain the segmented detection image.

The first site, the second site and the third site may be understood as sites constituting the sperm to be detected. In the embodiment of the present invention, the first portion, the second portion, and the third portion may be preset according to a scene, which is not specifically limited herein. Alternatively, the first location may be the sperm head of a sperm; the second location may be a midsection of the sperm; the third location may be the main segment of sperm (see figure 5).

The first value may be understood as a detection value corresponding to the first location. The second value may be understood as a detection value corresponding to the second location. The third value may be understood as a detection value corresponding to the third location. Alternatively, the first value may include a sperm head width, a sperm head length, and a head aspect ratio. The second value may include a mid-section length. The second value may comprise a main segment length.

The first preset interval may be understood as a preset interval corresponding to the first numerical value. The second preset interval may be understood as a preset interval corresponding to the second value. The third preset interval may be understood as a preset interval corresponding to the third numerical value. Optionally, the first preset interval may include a sperm head wide interval, a sperm head long interval, and a head aspect ratio interval. The second preset interval may include a middle length interval. The third preset interval may include a main segment length interval. In the embodiment of the present invention, the first preset interval, the second preset interval, and the third preset interval may be preset according to a scene requirement, which is not specifically limited herein. Illustratively, the sperm head wide interval may be 2 μm-3 μm, the sperm head long interval may be 3 μm-5 μm, and the head aspect ratio interval may be 1.3 to 1.8. The mid-length section may be 0.6 μm to 1.4 μm. The main segment length interval may be 44 μm-46 μm.

Specifically, by way of example, the segmentation detection image is processed through a semantic segmentation algorithm, and three parts of the sperm head, the middle section and the main section of the sperm corresponding to the segmentation detection image are determined; further, classifying the three parts by a classification algorithm to obtain sperm heads, middle sections and main sections of sperms corresponding to the segmentation detection images; still further, determining a sperm head width, a sperm head length, and a head aspect ratio corresponding to the sperm head; determining the length of the middle section corresponding to the middle section; determining the length of a main section corresponding to the main section; under the condition that the determined numerical values all meet the corresponding preset intervals, determining the morphological detection result corresponding to the sperm to be detected as a normal morphology; and under the condition that the determined numerical value does not meet the corresponding preset interval, determining the morphological detection result corresponding to the sperm to be detected as an abnormal morphology.

Optionally, the determining, based on the image to be detected, a motion detection result corresponding to the sperm to be detected includes:

determining the motion trail of the sperm to be detected based on the image to be detected;

determining a first movement result corresponding to the sperm to be detected according to the movement track, and determining a second movement result corresponding to the first movement result;

and determining a motion detection result corresponding to the sperm to be detected according to the first motion result and the second motion result.

The motion trajectory may be understood as the trajectory of the motion of the sperm to be detected.

The first movement result may be understood as a movement result of the sperm to be detected determined based on the movement track. Alternatively, the first movement result may be forward, non-forward, no movement, etc.

Specifically, optionally, a first movement result corresponding to the sperm to be detected is determined by a wild horse optimization algorithm (Wild horse optimizer, WHO).

The second movement result may be understood as a movement result corresponding to the first movement result. Alternatively, the second motion result may be a fast, slow or no speed. It will be appreciated that when the first movement result is forward, it may be further determined that the second movement result is fast or slow. When the first motion result is not forward or not motion, the corresponding second motion result may be velocity-free.

Optionally, the determining the motion trail of the sperm to be detected based on the image to be detected includes:

aiming at the image to be detected, a detection frame corresponding to a preset part is obtained by identifying the preset part of the sperm to be detected, and a detection frame center point of the detection frame is determined;

and connecting the center points of the detection frames of each image to be detected corresponding to the sperms to be detected to obtain the movement track of the sperms to be detected.

The predetermined location may be understood as a location to be identified. In the embodiment of the present invention, the preset portion may be preset according to a scene requirement, which is not specifically limited herein. Alternatively, the preset portion may be the first portion. Illustratively, the predetermined location may be a sperm head.

The detection frame may be understood as a frame to be detected corresponding to the preset portion. Optionally, the detection frame may be a rectangular frame corresponding to the preset portion.

The center point of the detection frame can be understood as the center point corresponding to the detection frame.

S250, determining a target detection result of the sperm to be detected according to the motion detection result and the morphological detection result.

According to the technical scheme, the images to be detected in the preset time period are determined for each sperm to be detected, and a preset number of images to be detected are obtained based on the images to be detected; determining a motion detection result corresponding to the sperm to be detected based on the image to be detected, and determining a morphology detection result corresponding to the sperm to be detected based on the image to be detected; and determining a target detection result of the sperm to be detected according to the motion detection result and the morphology detection result. The motion detection result and the morphology detection result corresponding to the sperm to be detected can be accurately determined, so that the accuracy of the target detection result is improved.

Example III

Fig. 6 is a schematic structural diagram of a sperm optimizing and detecting device according to a third embodiment of the present invention. As shown in fig. 6, the apparatus includes: a microfluidic processing module 310, a liquid flow processing module 320, and a neural network module 330.

The microfluidic processing module 310 is configured to obtain original semen, and perform microfluidic processing on the original semen through the microfluidic chip to obtain primary semen; the liquid flow processing module 320 is configured to perform liquid flow processing on the preliminary semen by using a flow cytometer to obtain sperm to be detected; the neural network module 330 is configured to determine a motion detection result and a morphology detection result of each sperm to be detected, and obtain a target detection result.

Optionally, the neural network module 330 includes: the device comprises an image acquisition sub-module, an image detection sub-module and a result determination sub-module.

The image acquisition sub-module is used for determining images to be detected in a preset time period for each sperm to be detected, and obtaining a preset number of images to be detected based on the images to be detected;

the image detection submodule is used for determining a motion detection result corresponding to the sperm to be detected based on the image to be detected and determining a morphological detection result corresponding to the sperm to be detected based on the image to be detected;

the result determination submodule is used for determining a target detection result of the sperm to be detected according to the motion detection result and the morphological detection result.

Optionally, the image detection sub-module is configured to:

Optionally, the image detection sub-module includes: a motion trail determination unit, a motion result detection unit and a motion result determination unit.

The motion trail determining unit is used for determining the motion trail of the sperm to be detected based on the image to be detected;

the motion result detection unit is used for determining a first motion result corresponding to the sperm to be detected according to the motion trail and determining a second motion result corresponding to the first motion result;

the movement result determining unit is used for determining a movement detection result corresponding to the sperm to be detected according to the first movement result and the second movement result.

Optionally, the motion trail determining unit is configured to:

Optionally, the sperm optimizing and detecting device further comprises: and a distribution processing module.

The distribution processing module is used for obtaining a single sperm to be detected by uniformly distributing the sperm to be detected before the target detection result is obtained by determining the movement detection result and the morphological detection result of each sperm to be detected.

Optionally, the sperm optimizing and detecting device further comprises: and a type determining module.

The type determining module is used for determining the sperm type of the sperm to be detected according to the target detection result.

The sperm optimizing and detecting device provided by the embodiment of the invention can execute the sperm optimizing and detecting method provided by any embodiment of the invention, and has the corresponding functional modules and beneficial effects of the executing method.

Example IV

Fig. 7 shows a schematic diagram of the structure of an electronic device 10 that may be used to implement an embodiment of the invention. Electronic devices are intended to represent various forms of digital computers, such as laptops, desktops, workstations, personal digital assistants, servers, blade servers, mainframes, and other appropriate computers. Electronic equipment may also represent various forms of mobile devices, such as personal digital processing, cellular telephones, smartphones, wearable devices (e.g., helmets, glasses, watches, etc.), and other similar computing devices. The components shown herein, their connections and relationships, and their functions, are meant to be exemplary only, and are not meant to limit implementations of the inventions described and/or claimed herein.

As shown in fig. 7, the electronic device 10 includes at least one processor 11, and a memory, such as a Read Only Memory (ROM) 12, a Random Access Memory (RAM) 13, etc., communicatively connected to the at least one processor 11, in which the memory stores a computer program executable by the at least one processor, and the processor 11 may perform various appropriate actions and processes according to the computer program stored in the Read Only Memory (ROM) 12 or the computer program loaded from the storage unit 18 into the Random Access Memory (RAM) 13. In the RAM 13, various programs and data required for the operation of the electronic device 10 may also be stored. The processor 11, the ROM 12 and the RAM 13 are connected to each other via a bus 14. An input/output (I/O) interface 15 is also connected to bus 14.

Various components in the electronic device 10 are connected to the I/O interface 15, including: an input unit 16 such as a keyboard, a mouse, etc.; an output unit 17 such as various types of displays, speakers, and the like; a storage unit 18 such as a magnetic disk, an optical disk, or the like; and a communication unit 19 such as a network card, modem, wireless communication transceiver, etc. The communication unit 19 allows the electronic device 10 to exchange information/data with other devices via a computer network, such as the internet, and/or various telecommunication networks.

The processor 11 may be a variety of general and/or special purpose processing components having processing and computing capabilities. Some examples of processor 11 include, but are not limited to, a Central Processing Unit (CPU), a Graphics Processing Unit (GPU), various specialized Artificial Intelligence (AI) computing chips, various processors running machine learning model algorithms, digital Signal Processors (DSPs), and any suitable processor, controller, microcontroller, etc. The processor 11 performs the various methods and processes described above, such as sperm cell optimization and detection methods.

In some embodiments, the sperm optimization and detection method may be implemented as a computer program tangibly embodied on a computer-readable storage medium, such as storage unit 18. In some embodiments, part or all of the computer program may be loaded and/or installed onto the electronic device 10 via the ROM 12 and/or the communication unit 19. When the computer program is loaded into RAM 13 and executed by processor 11, one or more of the steps of the sperm cell optimization and detection method described above may be performed. Alternatively, in other embodiments, the processor 11 may be configured to perform sperm optimization and detection methods in any other suitable manner (e.g., by means of firmware).

Various implementations of the systems and techniques described here above may be implemented in digital electronic circuitry, integrated circuit systems, field Programmable Gate Arrays (FPGAs), application Specific Integrated Circuits (ASICs), application Specific Standard Products (ASSPs), systems On Chip (SOCs), load programmable logic devices (CPLDs), computer hardware, firmware, software, and/or combinations thereof. These various embodiments may include: implemented in one or more computer programs, the one or more computer programs may be executed and/or interpreted on a programmable system including at least one programmable processor, which may be a special purpose or general-purpose programmable processor, that may receive data and instructions from, and transmit data and instructions to, a storage system, at least one input device, and at least one output device.

A computer program for carrying out methods of the present invention may be written in any combination of one or more programming languages. These computer programs may be provided to a processor of a general purpose computer, special purpose computer, or other programmable data processing apparatus, such that the computer programs, when executed by the processor, cause the functions/acts specified in the flowchart and/or block diagram block or blocks to be implemented. The computer program may execute entirely on the machine, partly on the machine, as a stand-alone software package, partly on the machine and partly on a remote machine or entirely on the remote machine or server.

In the context of the present invention, a computer-readable storage medium may be a tangible medium that can contain, or store a computer program for use by or in connection with an instruction execution system, apparatus, or device. The computer readable storage medium may include, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any suitable combination of the foregoing. Alternatively, the computer readable storage medium may be a machine readable signal medium. More specific examples of a machine-readable storage medium would include an electrical connection based on one or more wires, a portable computer diskette, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing.

To provide for interaction with a user, the systems and techniques described here can be implemented on an electronic device having: a display device (e.g., a CRT (cathode ray tube) or LCD (liquid crystal display) monitor) for displaying information to a user; and a keyboard and a pointing device (e.g., a mouse or a trackball) through which a user can provide input to the electronic device. Other kinds of devices may also be used to provide for interaction with a user; for example, feedback provided to the user may be any form of sensory feedback (e.g., visual feedback, auditory feedback, or tactile feedback); and input from the user may be received in any form, including acoustic input, speech input, or tactile input.

The systems and techniques described here can be implemented in a computing system that includes a background component (e.g., as a data server), or that includes a middleware component (e.g., an application server), or that includes a front-end component (e.g., a user computer having a graphical user interface or a web browser through which a user can interact with an implementation of the systems and techniques described here), or any combination of such background, middleware, or front-end components. The components of the system can be interconnected by any form or medium of digital data communication (e.g., a communication network). Examples of communication networks include: local Area Networks (LANs), wide Area Networks (WANs), blockchain networks, and the internet.

The computing system may include clients and servers. The client and server are typically remote from each other and typically interact through a communication network. The relationship of client and server arises by virtue of computer programs running on the respective computers and having a client-server relationship to each other. The server can be a cloud server, also called a cloud computing server or a cloud host, and is a host product in a cloud computing service system, so that the defects of high management difficulty and weak service expansibility in the traditional physical hosts and VPS service are overcome.

It should be appreciated that various forms of the flows shown above may be used to reorder, add, or delete steps. For example, the steps described in the present invention may be performed in parallel, sequentially, or in a different order, so long as the desired results of the technical solution of the present invention are achieved, and the present invention is not limited herein.

The above embodiments do not limit the scope of the present invention. It will be apparent to those skilled in the art that various modifications, combinations, sub-combinations and alternatives are possible, depending on design requirements and other factors. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of the present invention.

Claims

1. A sperm cell optimization and detection method comprising:

2. The method of claim 1, wherein determining the movement detection result and the morphology detection result of each sperm to be detected to obtain the target detection result comprises:

determining images to be detected in a preset time period for each sperm to be detected, and obtaining a preset number of images to be detected based on the images to be detected;

determining a motion detection result corresponding to the sperm to be detected based on the image to be detected, and determining a morphology detection result corresponding to the sperm to be detected based on the image to be detected;

and determining a target detection result of the sperm to be detected according to the motion detection result and the morphology detection result.

3. The method according to claim 2, wherein the determining a morphological detection result corresponding to the sperm to be detected based on the image to be detected includes:

4. The method of claim 2, wherein the determining the motion detection result corresponding to the sperm to be detected based on the image to be detected comprises:

5. The method of claim 4, wherein the determining the motion profile of the sperm to be detected based upon the image to be detected comprises:

6. The method of claim 1, further comprising, prior to said obtaining a target test result by determining a movement test result and a morphology test result for each of said sperm to be tested:

7. The method as recited in claim 1, further comprising:

8. A sperm cell preference and detection device comprising:

9. An electronic device, the electronic device comprising:

at least one processor; and

the memory stores a computer program executable by the at least one processor to enable the at least one processor to perform the sperm cell optimization and detection method of any of claims 1-7.

10. A computer readable storage medium, characterized in that it stores computer instructions for causing a processor to execute the sperm optimization and detection method of any one of claims 1-7.