CN116115626A - Application of plant extract in improving pig reproductive performance - Google Patents
Application of plant extract in improving pig reproductive performance Download PDFInfo
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- CN116115626A CN116115626A CN202211605845.4A CN202211605845A CN116115626A CN 116115626 A CN116115626 A CN 116115626A CN 202211605845 A CN202211605845 A CN 202211605845A CN 116115626 A CN116115626 A CN 116115626A
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- 239000000419 plant extract Substances 0.000 title claims abstract description 10
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
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- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gynecology & Obstetrics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Genetics & Genomics (AREA)
- Pregnancy & Childbirth (AREA)
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- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
The invention provides an application of a plant extract in improving pig reproductive performance, and belongs to the technical field of pig reproduction. The plant extract provided by the invention is ophiopogon saponin B. Experiments show that the growth, migration and tube forming capacity of the trophoblastic cells treated by the ophiopogon saponin B are better than those of the non-treated cells, so that the ophiopogon saponin B can effectively improve the growth, migration and tube forming capacity of the trophoblastic cells, and the functions of the trophoblastic cells can be better played, and the possibility is provided for further improving the reproductive capacity of pigs.
Description
Technical Field
The invention belongs to the technical field of pig breeding, and particularly relates to application of a plant extract in improving pig breeding performance.
Background
Pork is soft and delicious in taste, so that the pork becomes a main animal food on a dining table of Chinese people. However, the existing sow variety in China has the problem of poor reproductive capacity. Therefore, how to effectively improve the reproductive capacity of live pigs is an urgent problem to be solved for the pig industry.
Placenta is an important organ for maintaining fetal growth and development, and consists of placenta trophoblast cells, capillary endothelial cells and basement membrane therebetween. The placenta is the only substance exchange channel between the gestation mother and the fetus of the mammal, plays a very important role in the growth and development of the fetus, plays important roles of immune barrier, substance transport and the like in the development process of the fetus, and is closely related to the growth and the function of placenta trophoblast cells. Thus, improving the growth and function of placental trophoblast cells would help to improve the reproductive capacity of live pigs.
The liriope spicata saponin B is a steroid glycoside extracted from low part of liriope spicata, and its molecular formula is C 41 H 64 O 13 CAS No. 182284-68-0. The prior study shows that the liriope spicata saponin B has a protective effect on cerebral ischemia injury and has a remarkable anticoagulation effect. However, to date, the art remains blank in terms of other effects of ophiopogonin B, such as modulation of trophoblastic cells.
Disclosure of Invention
The present invention is directed to a plant extract: the application of the liriope spicata saponin B in improving the reproductive performance of pigs can further improve the reproductive capacity of the pigs.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the invention provides an application of a plant extract in preparation of a preparation for improving pig reproductive performance, wherein the plant extract is liriope spicata saponin B.
Preferably, the formulation enhances growth of porcine trophoblast cells, promotes migration of porcine trophoblast cells, and promotes pipelining ability of porcine trophoblast cells.
Preferably, a pharmaceutically acceptable carrier is also included in the formulation.
Preferably, the pharmaceutically acceptable carrier comprises one or a mixture of several diluents, excipients, binders, fillers, surfactants or lubricants.
Preferably, the diluent is one or a mixture of more of calcium sulfate, dextrin, dextrates, kaolin and microcrystalline cellulose;
preferably, the excipient is one or a mixture of a plurality of lactose, mannitol and starch.
Preferably, the binder is one or a mixture of several of methylcellulose, polyvinylpyrrolidone, hydroxypropyl cellulose and hydroxypropyl methylcellulose.
Preferably, the formulation is one of solution, emulsion, capsule and powder.
Secondly, the invention provides application of liriope spicata saponin B in preparation of a preparation for improving growth of pig trophoblast cells.
Secondly, the invention provides application of liriope spicata saponin B in preparation of a preparation for improving cell migration of porcine trophoblasts.
Secondly, the invention provides application of liriope spicata saponin B in preparation of a preparation for improving the pipelining capability of pig trophoblast cells.
Preferably, a pharmaceutically acceptable carrier is also included in the formulation.
Preferably, the pharmaceutically acceptable carrier comprises one or a mixture of several diluents, excipients, binders, fillers, surfactants or lubricants.
Preferably, the diluent is one or a mixture of more of calcium sulfate, dextrin, dextrates, kaolin and microcrystalline cellulose;
preferably, the excipient is one or a mixture of a plurality of lactose, mannitol and starch.
Preferably, the binder is one or a mixture of several of methylcellulose, polyvinylpyrrolidone, hydroxypropyl cellulose and hydroxypropyl methylcellulose.
Preferably, the preparation is one of solution, emulsion, capsule and powder.
The invention has the beneficial effects that:
the invention provides a new application of liriope spicata saponin B in the aspect of regulating and controlling pig trophoblastic cells, and discovers that liriope spicata saponin B can effectively promote the growth, migration and tube forming capacity of pig trophoblastic cells, so that the liriope spicata saponin B can be prepared into a pharmaceutical preparation to better promote the growth of trophoblastic cells and exert the functions of the trophoblastic cells, thereby improving the possibility of improving the reproductive capacity of pigs.
Drawings
FIG. 1 is a graph showing the results of controlling cell migration of liriope spicata B with respect to porcine trophoblast pTr 2;
FIG. 2 shows the result of controlling the tube forming ability of liriope spicata saponin B on porcine trophoblast pTr 2.
Detailed Description
Example 1
The influence of liriope spicata saponin B on the growth of pig trophoblast pTr2 is detected by CCK-8 experiment
1. pTr2 cells are inoculated into 96-well plates according to 2000 cells per well, 100ul of DMEM/F12 complete medium is added into each well, and the cells are placed into a cell culture box for culture overnight;
2. discarding the culture medium, washing with PBS for 2 times, and adding serum-free DMEM/F12 culture medium for 24 hours;
3. the medium was discarded, and replaced with a DMEM/F12 medium containing 0. Mu.M, 2.5. Mu.M, 5. Mu.M, 10. Mu.M, 20. Mu.M, and 40. Mu.M of liriope-B, and the culture was carried out in a cell incubator for 72 hours, and the results of the detection were shown in tables 1 to 3 below.
TABLE 1 detection of OD values of trophoblast cells
| Concentration of | OD value |
| 0μM | 0.299±0.026 |
| 2.5μM | 0.372±0.026 * |
| 5μM | 0.453±0.024 ** |
| 10μM | 0.528±0.022 *** |
| 20μM | 0.506±0.026 *** |
| 40μM | 0.499±0.029 *** |
In the results of Table 1, when the concentration of liriope spicata saponin B was greater than or equal to 2.5. Mu.M, it was found that promotion of growth of porcine trophoblast pTr2 was achieved, and when the concentration of liriope spicata was 10. Mu.M, the promotion was maximized. From the above results, it can be seen that liriope spicata saponin B can effectively promote the growth of pig trophoblast pTr 2.
Example 2
Detection of the Effect of Liriope spicata saponin B on invasion of porcine trophoblast pTr2 by Transwell cell
1. Placing Matrigel gel frozen in a refrigerator at-80 ℃ overnight at 4 ℃, thawing into liquid state, and pre-cooling gun heads and the like used in experiments;
2. diluting Matrigel gel with serum-free culture medium according to the ratio of 1:8, fully mixing, and adding 50 mu l of diluted Matrigel into the upper chamber of the Transwell chamber;
3. pig trophoblast pTr2 cultured for 24h with 0. Mu.M and 10. Mu.M liriope spicatum saponin B was prepared as 2X 10 5 Per ml of cell suspension, cells were seeded into Transwell chambers in an amount of 200 μl of cell suspension per well;
4. adding 600 mu l of DMEM/F12 complete medium into the lower chamber of the Transwell chamber, and placing the Transwell chamber into a cell incubator for culturing;
5. after 24h of treatment, the Transwell chamber was removed from the cell culture plate and Matrigel was removed by gently scrubbing the upper chamber with a cotton swab;
6. after the cell was fixed in methanol for 20min, it was stained with 5% crystal violet for 20min and washed with PBS;
7. the results obtained by observation and photographing using an inverted microscope and counting are shown in fig. 1.
In FIG. 1, we can see that the number of cells passing through the cells is significantly increased compared with that of the cells not treated after the treatment with 10. Mu.M liriope spicatum saponin B, which indicates that the treatment of pig trophoblast pTr2 with liriope spicatum saponin B can effectively improve the invasiveness of the cells, thereby better functioning of the trophoblast cells.
Example 3
Influence of liriope spicata saponin B on pig trophoblast pTr2 angiogenesis by tube-forming experiment
1. Placing Matrigel gel frozen in a refrigerator at-80 ℃ overnight at 4 ℃, thawing into liquid state, and pre-cooling gun heads and the like used in experiments;
2. in a 96-well plate, 50 μl of melted Matrigel glue is added to each well, and the mixture is placed in an incubator to be coated for 30min;
3. pig trophoblast pTr2 cultured for 24h with 0. Mu.M and 10. Mu.M liriope spicata saponin B was prepared as 5X 10 5 Per ml of cell suspension 1X 10 per well 5 Amount of individual cells were seeded into the culture plates;
4. after the culture plates were placed in a fine incubator for continuous culture for 6 hours, photographing was performed under an inverted microscope, and the results obtained are shown in fig. 2.
In fig. 2, we can see that after the pig trophoblast pTr2 is treated by 10 μm of ophiopogonin B, the tube forming ability of the cells is significantly enhanced, so that the angiogenesis ability of the pig trophoblast cells can be effectively improved, thereby forming new blood vessels, increasing blood flow and realizing nutrition and waste exchange by replacement.
By combining the results of examples 1-3, we can conclude that the use of liriope spicata saponin B to treat porcine trophoblast cells can enhance the ability of the cells to grow, migrate and angiogenesis, thereby better achieving the growth and function of the trophoblast cells.
Claims (9)
1. The application of a plant extract in preparing a preparation for improving pig reproductive performance is characterized in that the plant extract is ophiopogonin B.
2. The use of claim 1, wherein the formulation enhances growth of porcine trophoblast cells, promotes migration of porcine trophoblast cells, and promotes pipelining ability of porcine trophoblast cells.
3. The use of claim 1, wherein the formulation further comprises a pharmaceutically acceptable carrier.
4. The use according to claim 3, wherein the formulation is in the form of one of a solution, an emulsion, a capsule, and a powder.
5. Application of liriope spicata saponin B in preparation of preparation for improving growth of pig trophoblast cells is provided.
6. Application of liriope spicata saponin B in preparation of preparation for improving pig trophoblast cell migration is provided.
7. Application of liriope spicata saponin B in preparation of preparation for improving pig trophoblast tube forming ability is provided.
8. The use according to any one of claims 5 to 7, wherein the formulation further comprises a pharmaceutically acceptable carrier.
9. The use according to any one of claims 5 to 7, wherein the formulation is in the form of one of a solution, an emulsion, a capsule, a powder.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103190536A (en) * | 2013-04-24 | 2013-07-10 | 沈阳双胞胎饲料有限公司 | Safe and environment-friendly high-efficiency pig feed additive |
| CN105816563A (en) * | 2016-05-20 | 2016-08-03 | 山东德州神牛药业有限公司 | Application of traditional Chinese medicine composition in preparing drugs for improving reproductive performance of sows |
| CN107258689A (en) * | 2017-05-19 | 2017-10-20 | 安徽禾泉农庄生态农业有限公司 | It is a kind of to do kind of a cultural method for black pig |
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| CN112741205A (en) * | 2021-01-14 | 2021-05-04 | 湖南农大追求饲料科技有限公司 | Feed additive for improving reproductive capacity of sows and preparation method thereof |
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