CN116114878A

CN116114878A - Plant source composition

Info

Publication number: CN116114878A
Application number: CN202211580569.0A
Authority: CN
Inventors: 唐俊; 徐世杰; 王维; 郎朗; 廖琪林
Original assignee: Nanjing Niubang Biotechnology Co ltd
Current assignee: Nanjing Niubang Biotechnology Co ltd
Priority date: 2022-12-09
Filing date: 2022-12-09
Publication date: 2023-05-16
Also published as: WO2024120362A1

Abstract

The invention discloses a plant source composition, which comprises, by weight, 30% -50% of a first plant component, 37% -57% of a second plant component and 5% -20% of a third plant component. The plant source composition has high antioxidant capacity, can effectively remove free radicals of human bodies, and can be used as a composite dietary supplement for supplementing nutrients necessary for the human bodies.

Description

Plant source composition

Technical Field

The invention belongs to the technical field of health-care foods and dietary supplements, and particularly relates to a plant source composition with high antioxidant capacity.

Background

Free radicals (Free radicals) are intermediate metabolites of various biochemical reactions in human vital activities, have high chemical activities, are effective defense systems of organisms, and affect the vital activities of the organisms if a certain level cannot be maintained. Various free radicals are continuously generated in the metabolic process of human body, including antioxidant free radicals (ORAC), hydroxyl free radicals (hoac), superoxide anion free radicals (SORAC), nitrite peroxide (NORAC), singlet oxygen free radicals (SOAC), and when the free radicals are excessive in the body, a series of biological reactions are initiated, which lead to physical decline and the occurrence of lesions. The antioxidant can effectively remove free radicals, and prevent or inhibit various diseases caused by excessive free radicals.

The antioxidant with single component can not play a role in comprehensively removing free radicals with different properties due to the limitation of the structure, and has low antioxidant activity when used singly and easy toxic and side effects when the dosage is large. Through reasonable formula and proportion, a plurality of antioxidant components can generate synergistic effect, and the complementary effect on the structure-activity relationship can be utilized to exert stronger effect than a single antioxidant. The composite antioxidant can be regenerated through mutual repair to form a redox circulation system, so that the antioxidant activity is obviously stronger than that of the monomeric antioxidant. However, antioxidants of different structural types have different activities, and even antioxidants of the same structural type have activities affected by the number and positions of substituents. The antioxidant synergism or antagonism between the antioxidant components is affected by the structure, concentration, reaction system, etc. of the antioxidant. An improper formulation results in antagonism, and antagonism is strongly correlated with its formulation. Therefore, the research on the synergistic effect among antioxidants, and the search of the efficient and low-toxicity compound natural antioxidant have great significance for efficiently utilizing the antioxidants.

The existing antioxidant single component or composition cannot achieve high-level antioxidant free radical absorption capacity, so that development of a product of a plant source composition with high antioxidant capacity is needed to remove human free radicals efficiently, various diseases caused by excessive free radicals in a human body are prevented or stopped, and the product has a very broad market prospect.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a product of a plant source composition with high antioxidant capacity to effectively remove free radicals of human bodies, and the product can be used as a composite dietary supplement for supplementing nutrients necessary for the human bodies to achieve ideal health care effect.

In one aspect, the present invention provides a plant-derived composition comprising a first plant component, a second plant component, a third plant component, the first plant component selected from one or more of the following: quercetin, green tea extract, broccoli sprout extract, onion extract, apple extract, tomato extract, broccoli extract, carbomer extract, acerola cherry extract, blueberry extract, sweet cherry extract, raspberry extract, spinach extract, cherokee rose extract, kale extract, blackberry extract, cowberry extract; the second plant component is selected from one or more of the following: olive extract, clove extract, resveratrol; the third plant component is selected from one or more of the following: rosemary extract, grape seed extract.

In some embodiments, the plant-derived composition comprises 30% -50% by weight of the above first plant component, 37% -57% by weight of the above first plant component, 5% -20% by weight of the above third plant component. Preferably, the first component is present in an amount of 33% -49% by weight, 35% -47% by weight, 38% -45% by weight, the second component is present in an amount of 40% -56% by weight, 43% -53% by weight, 45% -50% by weight, the third component is present in an amount of 7% -18% by weight, 9% -16% by weight, 11% -14% by weight,

in some embodiments, the plant-derived composition further comprises from 0.5% to 2% by weight of a fourth plant component selected from one or more of the following: turmeric extract, garlic extract, basil extract, oregano extract, cinnamon extract, carrot extract, elderberry extract, mangosteen extract.

In some embodiments, the plant-derived composition further comprises from 0.5% to 2% by weight of a fifth plant component selected from one or more of the following: basil extract, oregano extract, cinnamon extract and elderberry extract.

In some embodiments, the plant-derived composition further comprises fillers, glidants, and additives.

In some embodiments, the filler comprises one or more of the following: resistant dextrins, maltodextrins, lactose; the glidant comprises one or more of the following: silicon dioxide, magnesium stearate, talcum powder and tricalcium phosphate; the additive comprises one or more of the following: ergothioneine, L-ascorbic acid, sodium D-erythorbate, and tea polyphenols.

In some embodiments, the first plant component is quercetin and a kale extract; the second plant component is olive extract; the third plant component is rosemary extract.

In some embodiments, the first plant component is quercetin and a kale extract; the second plant component is olive extract; the third plant component is rosemary extract; the fourth plant component is turmeric extract; the fifth plant component is cinnamon extract.

In some embodiments, the above composition further comprises a resistant dextrin, silica, ergothioneine.

In another aspect, the present invention provides a method of using a plant-derived composition as described above, the method comprising: the plant source composition is administered to a subject in need thereof in an amount of 0.1g to 200g per day.

In some embodiments, the botanical composition may be 0.1-150g,0.2-100g,0.5-50g,1-20g,2-15g,4-10g,6-9g per day. In some embodiments, the plant-based composition may be applied one or more times per day.

In some embodiments, the plant-based composition is prepared as a solid formulation or a liquid formulation. In some embodiments, the plant-based composition is prepared in powder form. In some embodiments, the plant-based composition may be administered alone or in combination with other nutritional supplements, foods, beverages, or mixtures. In some embodiments, the plant-based composition may be mixed in powder form in yogurt or breading.

In some embodiments, the use of the botanical composition of the present invention for preparing a food, beverage, nutraceutical, dietary supplement, cosmetic.

The plant source composition is beneficial to balanced collocation among nutrients required by human bodies; mainly from various plants such as fruits, vegetables and the like, is easy to obtain, has green and environment-friendly sources, is safe in components and is easy to be absorbed by intestinal tracts; the flavor is good, and the adaptability of a user is improved; the high antioxidation capability which cannot be achieved by single plant components or the components of the existing compound composition on the market is achieved, the free radicals of the human body can be effectively removed, and various diseases caused by the surplus free radicals in the human body can be prevented or stopped.

Detailed Description

Reference will now be made in detail to the preferred embodiments of the present invention, examples of which are further described. While the invention will be described in conjunction with the preferred embodiments, it will be understood that they are not intended to limit the invention to these embodiments. On the contrary, the invention is intended to cover alternatives, modifications and equivalents, which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the detailed description of the present invention, numerous specific details are set forth in order to provide a thorough understanding of the present invention. It will be apparent, however, to one skilled in the art that the present invention may be practiced without these specific details. In other instances, well-known methods, procedures, components, and other features have not been described in detail so as not to unnecessarily obscure aspects of the present invention.

As used herein, the term "or" is intended to include "and" or ". In other words, the term "or" may also be replaced with "and/or".

As used herein, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

As used herein, the terms "comprises" or "comprising" or variations thereof mean the following, wherein the term is used in its non-limiting sense to refer to items following the inclusion of the word, but not to exclude items not specifically mentioned. It also includes the more restrictive verbs 'consisting essentially of … …' and 'consisting of … …'.

As used herein, the terms "subject" or "individual" are used interchangeably to refer to any subject to which the methods and compositions of the present disclosure may be applied or administered. The subject is likely to have a disease or disorder, but the subject need not be ill to benefit from the methods and compositions of the present disclosure. Thus, any subject may take the disclosed compositions or be the recipient of the disclosed methods. Herein, the term "subject" refers to animals (e.g., birds, reptiles, and mammals). In certain embodiments, the subject can be a mammal including a non-primate (e.g., camel, donkey, zebra, cow, horse, cat, dog, rat, and mouse) and a primate (e.g., monkey, chimpanzee, human). In certain embodiments, the subject may be a non-human mammal. In other embodiments, the subject may be a human.

As used herein, the term "administering" refers to the process of delivering the disclosed compositions or active ingredients to a subject. The compositions of the present invention may be administered in a variety of ways to exert the desired effect, including oral, intragastric and parenteral (i.e. intravenous and intraarterial, as well as other suitable parenteral routes), and the like.

As used herein, the term "plant component" is any material based on or derived from any kind of plant matter, meaning a material that contains the whole plant itself, a portion of the plant itself (e.g., leaves, roots, bark, meat, juice, etc.), or a processed product thereof, and may include, for example, plant extracts, plant concentrates, dried plants, plant juices, plant powders, plant fibers, and the like. In some embodiments, the plant component may be based on fruits, vegetables, common medicinal and edible traditional Chinese medicines, and the like.

The plant-based composition is simple and convenient to prepare, and can be prepared by uniformly mixing the raw materials. In a preferred embodiment, the method of preparing a plant-based composition of the invention comprises the steps of: firstly, uniformly mixing raw materials with low content in raw materials, and then, incrementally mixing the raw materials with high content in equal amount; more preferably, the raw materials are mixed in a three-dimensional mixing manner, and the mixing time is 20-30 minutes. In the preparation method of the present invention, functional components may be added appropriately in the above steps depending on the nature of the raw materials.

The technical features, implementation methods and advantageous effects of the present invention will be described in further detail below with reference to specific embodiments, and the embodiments described below are only some embodiments, but not all embodiments of the present invention. The materials and reagents described in the examples below are commercially available, unless otherwise specified.

Examples

These examples describe exemplary plant-based compositions.

Example 1 screening of plant extracts with high antioxidant capacity

ORAC value determination and screening: sample solution pretreatment: about 20mg of the plant extract is weighed, placed in a 50mL measuring flask, added with 30mL of 70% ethanol for ultrasonic treatment for 40 minutes, and the volume of the 70% ethanol is fixed to the scale. Transfer to a centrifuge tube, centrifuge at 4000R for 20 minutes, and collect supernatant. The supernatant was taken and diluted continuously (diluent: PBS) to within a reasonable linear range. PBS preparation: 8g of sodium chloride, 0.2g of KCL,0.27g of potassium dihydrogen phosphate, 3.58g of disodium hydrogen phosphate and 39mg of DTPA were weighed, placed in 1L of solution and stored at room temperature. Trolox linear formulation (mother liquor concentration 5 mM): the mother liquor was taken at 5mM and 10. Mu.L, and 240. Mu.L of PBS was added to prepare a 0.2mM solution.

The operation is described according to the kit: the microplate reader was turned on and the incubation temperature was set at 37 ℃. The 4X-fold diluent was diluted with deionized water to a 1X analytical diluent (assay diluent). The 100X fluorescent probe was diluted with 1X diluent to a 1X fluorescent probe. 25. Mu.L of each of PBS, sample solution and control solution was added to a 96-well plate. 150 μl of 1X fluorescent probe was added to a 96-well plate. The 96-well plate was transferred to a 37 ℃ microplate reader in the dark and incubated for 30 minutes. The microplate reader parameters were set up (run time 60min, read one value at 2min intervals; EX/em=480/520 nm; intensity: medium-high, sample placement area, shaking). 80mg/ml starter was formulated with PBS. 25 μl of the formulated starter was immediately taken and added to a 96-well plate. And immediately placing the sample into an enzyme label instrument for spot operation.

Screening different plant extracts by oxygen radical scavenging assay (ORAC) to obtain quercetin, green tea extract, broccoli sprout extract, onion extract, apple extract, tomato extract, broccoli extract, carbomer extract, acerola extract, blueberry extract, sweet cherry extract, raspberry extract, spinach extract, wild cherry extract, collard extract, blackberry extract, cowberry extract, etc. or their combination with high ORAC value, preferably 20,000 μmole TE/g.

HORAC value determination and screening: sample solution pretreatment: about 20mg of the plant extract is weighed, placed in a 50mL measuring flask, added with 30mL of 70% ethanol for ultrasonic treatment for 40 minutes, and the volume of the 70% ethanol is fixed to the scale. Transfer to a centrifuge tube, centrifuge at 4000R for 20 minutes, and collect supernatant. The supernatant was taken and diluted continuously (diluent: PBS) to within a reasonable linear range. PBS preparation: 8g of sodium chloride, 0.2g of KCL,0.27g of potassium dihydrogen phosphate, 3.58g of disodium hydrogen phosphate and 39mg of DTPA were weighed, placed in 1L of solution and stored at room temperature. Gallicic acid Linear formulation (mother liquor concentration 5 mM): the mother liquor was taken at 5mM and 10. Mu.L, and 240. Mu.L of PBS was added to prepare a 0.2mM solution.

The operation is described according to the kit: the microplate reader was turned on and the incubation temperature was set at 37 ℃. The 4X-fold diluent was diluted with deionized water to a 1X analytical diluent (assay diluent). The 100X fluorescent probe was diluted with 1X diluent to a 1X fluorescent probe. 25. Mu.L of each of PBS, sample solution and control solution was added to a 96-well plate. 140. Mu.L of 1 Xfluorescent probe was added to a 96-well plate. The 96-well plate was transferred to a 37 ℃ microplate reader in the dark and incubated for 30 minutes. The microplate reader parameters were set up (run time 60min, read one value at 2min intervals; EX/em=480/520 nm; intensity: automatic, sample placement area, shaking). The 5X starter was diluted with deionized water to a 1X solution (hydroxyl radical initiator). mu.L of 1 Xstarter solution was added to a 96-well plate. 20 mu L of Fenton reagent solution is taken and added into a 96-well plate, and the mixture is immediately placed into an enzyme-labeled instrument for spot operation.

Different plant extracts are screened by adopting a hydroxyl radical scavenging assay (HORAC), and the olive extract, the clove extract, the resveratrol and the like or the combination thereof are found to have higher HORAC value, and the preferable scheme can reach 11,000 mu mole TE/g.

SORAC value determination and screening: sample solution pretreatment: about 20mg of the plant extract is weighed, placed in a 50mL measuring flask, added with 30mL of 70% ethanol for ultrasonic treatment for 40 minutes, and the volume of the 70% ethanol is fixed to the scale. Transfer to a centrifuge tube, centrifuge at 4000R for 20 minutes, and collect supernatant. The supernatant was taken and diluted continuously (diluent: PBS) to within a reasonable linear range. PBS preparation: 8g of sodium chloride, 0.2g of KCL,0.27g of potassium dihydrogen phosphate, 3.58g of disodium hydrogen phosphate and 39mg of DTPA were weighed, placed in 1L of solution and stored at room temperature. Trolox linear formulation (mother liquor concentration 2mM, molecular weight= 250.29): a TROLOX solution was prepared at a concentration of 2 mM: trolox 5mg was weighed and dissolved (20 mM) in DMSO1 mL. 0.5mL was added to 4.5mL of LPBS.

The microplate reader was turned on and the incubation temperature was set at 37 ℃. Xanthine solution: xanthine 15mg is weighed, placed in a 100mL measuring flask, added with 5mL of 0.1N sodium hydroxide, ultrasonically dissolved, and then added with PBS buffer solution to dilute to a fixed volume to a scale. Diethyl pyridine fluorescent probe mother liquor: weighing 5mg of diethyl ether, adding 2mL of acetonitrile, diluting and dissolving, and preserving at a low temperature of minus 20 ℃. The diethyl pyridine fluorescent working solution: 20. Mu.L of the fluorogenic probe mother liquor of the dihydroethidium is precisely measured, and 2ml of xanthine solution is added. 25. Mu.L of each of PBS, sample solution and control solution was added to a 96-well plate. 150 μl of the working solution of the fluoroethylene-dihydropyridine probe is added into a 96-well plate, the 96-well plate is transferred to a 37 ℃ microplate reader in a dark place, incubated for 20 minutes, and the parameter setting of the microplate reader is completed (the running time is 40 minutes, a numerical value is read at intervals of 2 minutes; EX/EM=470/584 nm; intensity: medium and high, sample placement area, shaking). Xanthine oxidase solution: 3.5mg of xanthine oxidase (SIGMA 0.08 u/mg) was weighed, 0.5ml of PBS was added for dissolution, and the mixture was uniformly mixed, 25ul of xanthine oxidase solution was added to a 96-well plate, and immediately placed into an microplate reader for spot running.

Different kinds of plant extracts were screened using a hydroxyl radical scavenging assay (SORAC) and found to have a high SORAC value, preferably up to 5,000. Mu. Mole TE/g, of olive extract, rosemary extract, grape seed extract, etc. or a combination thereof.

NORAC value determination and screening: sample solution pretreatment: about 20mg of the plant extract is weighed, placed in a 50mL measuring flask, added with 30mL of 70% ethanol for ultrasonic treatment for 40 minutes, and the volume of the 70% ethanol is fixed to the scale. Transfer to a centrifuge tube, centrifuge at 4000R for 20 minutes, and collect supernatant. The supernatant was taken and diluted continuously (diluent: PBS) to within a reasonable linear range. PBS preparation: 8g of sodium chloride, 0.2g of KCL,0.27g of potassium dihydrogen phosphate, 3.58g of disodium hydrogen phosphate and 39mg of DTPA were weighed, placed in 1L of solution and stored at room temperature. Trolox linear formulation (mother liquor concentration 2mM, molecular weight= 250.29): a TROLOX solution was prepared at a concentration of 2 mM: trolox 5mg was weighed and dissolved (20 mM) in DMSO1 mL. 0.5mL was added to 4.5mL of LPBS.

Standard linearity	trolox(μL)	PBS(μL)	Concentration (μm)
				1	100	900	200
2	50	950	100
				3	25	975	50
4	Taking 2, 90 mu L	210	30
				5	Taking 2, 80 mu L	320	20
6	Taking 5, 100 mu L	100	10
				7	6, 100 mu L of	100	5
8	Taking 5, 20 mu L	180	2
				9	0	4500	0

The microplate reader was turned on and the incubation temperature was set at 37 ℃. DHR-123 fluorescence stock formulation (5 mM): about 3.46mg of DHR-123 was precisely weighed, and 2mLN-N dimethylformamide was added thereto for storage at-80 ℃. DHR-123 fluorescent working solution (12.5 μm): precisely measuring 10 mu L of fluorescence mother solution, adding 4mLPBS and uniformly mixing. SIN-1 starter mother liquor: precisely weighing 2mg of SIN-1, adding 5mLN-N dimethylformamide, and preserving at-80deg.C. SIN-1 starter working solution: precisely weighing 40 mu L, adding 2mLPBS, and mixing uniformly. 25. Mu.L of each of PBS, sample solution and control solution was added to a 96-well plate. The microplate reader parameters were set up (run time 30min, read a number at 2min intervals; EX/em=470/525 nm; intensity: automatic, sample placement area, shaking) and 150 μl of DHR-123 working solution fluorescent probe was added to a 96-well plate. 25 μl of SIN-1 promoter working solution was added to a 96-well plate. And immediately placing the sample into an enzyme label instrument for spot operation.

Screening of different plant extracts using superoxide anion radical scavenging assay (NORAC) has found that turmeric extract, garlic extract, basil extract, oregano extract, cinnamon extract, carrot extract, elderberry extract, mangosteen extract, etc., or combinations thereof, have a high NORAC value, preferably up to 1400 μmole TE/g.

SOAC value determination and screening: sample solution pretreatment: about 20mg of the plant extract is weighed, placed in a 50mL measuring flask, added with 30mL of 70% ethanol for ultrasonic treatment for 40 minutes, and the volume of the 70% ethanol is fixed to the scale. Transfer to a centrifuge tube, centrifuge at 4000R for 20 minutes, and collect supernatant. The supernatant was taken and diluted continuously (diluent: PBS) to within a reasonable linear range. PBS preparation: 8g of sodium chloride, 0.2g of KCL,0.27g of potassium dihydrogen phosphate, 3.58g of disodium hydrogen phosphate and 39mg of DTPA were weighed, placed in 1L of solution and stored at room temperature. Trolox linear formulation (mother liquor concentration 2mM, molecular weight= 250.29): a TROLOX solution was prepared at a concentration of 2 mM: trolox 5mg was weighed and dissolved (20 mM) in DMSO1 mL. 0.5mL was added to 4.5mL of LPBS.

The microplate reader was turned on and the incubation temperature was set at 37 ℃. Diethyl pyridine fluorescent probe mother liquor: weighing 5mg of diethyl ether, adding 2mL of acetonitrile, diluting and dissolving, and preserving at a low temperature of minus 20 ℃. The diethyl pyridine fluorescent working solution: 150 μl of the fluorogenic probe mother liquor of the dihydroethandine is precisely measured, and 2mL of PBS is added for uniform mixing. Preparing a hydrogen peroxide solution: precisely measuring 1.5mL of 30% hydrogen peroxide, placing in a 50mL measuring flask, and adding PBS to dilute to a certain volume to a scale. Preparing a sodium molybdate solution: precisely weighing 40mg of sodium molybdate, placing into a 50mL measuring flask, adding PBS for ultrasonic dissolution, and diluting to a constant volume to a scale. 25. Mu.L of each of PBS, sample solution and control solution was added to a 96-well plate. 125. Mu.L of the fluorescent probe working solution was added to a 96-well plate. The 96-well plate was transferred to a 37 ℃ microplate reader in the dark and incubated for 60 minutes. The microplate reader parameters were set up (run time 30min, read one value at 2min intervals; EX/em=523/612 nm; intensity: medium-high, sample placement area, shaking). 25 mu L of hydrogen peroxide solution is added into a 96-well plate, 25 mu L of sodium molybdate solution is added into the 96-well plate, and the mixture is immediately put into an enzyme-labeled instrument for spot operation.

Screening of different plant extracts by singlet oxygen radical assay (SOAC) was performed and found that the extract of Leer, oregano, cinnamomum cassia, sambucus nigra, etc. or combinations thereof had a higher SOAC value, preferably up to 4,000 μmole TE/g.

Example 2

The plant source composition is prepared from the following components:

quercetin 35%; collard extract 6%;

48% of olive leaf extract; 11% of rosemary extract.

Example 3

The plant source composition is prepared from the following components:

quercetin 32%; 5% of collard extract;

50% of olive leaf extract; rosemary extract 13%.

Example 4

The plant source composition is prepared from the following components:

apple extract 22%; blackberry extract 25%;

20% of clove extract; resveratrol 20%;

13% of grape seed extract.

Example 5

The plant source composition is prepared from the following components:

example 6

Selecting one or more first plant components including quercetin, green tea extract, broccoli sprout extract, onion extract, apple extract, tomato extract, broccoli extract, carbomer extract, acerola extract, blueberry extract, sweet cherry extract, raspberry extract, spinach extract, cherokee rose extract, kale extract, blackberry extract, cowberry fruit extract, in an amount of 30-50% by weight; selecting one or more second plant components including olive extract, clove extract and resveratrol, wherein the content of the second plant components is 37-57% by weight; selecting one or more third plant components, including rosemary extract and grape seed extract, wherein the content by weight is 5% -20%; the total weight of the composition was 100%.

Example 7

The plant source composition is prepared from the following components:

quercetin 35%; 5% of collard extract;

47% of olive leaf extract; 10% of rosemary extract;

turmeric extract 1.5%; 1.5% of cinnamon extract.

Example 8

The plant source composition is prepared from the following components:

example 9

The plant source composition is prepared from the following components:

example 10

The plant source composition is prepared from the following components:

example 11

The plant source composition is prepared from the following components:

quercetin 35%; 47% of olive leaf extract;

10% of rosemary extract; 5% of collard extract;

clove extract, green tea extract, onion extract, acerola extract, peppermint leaf extract, grape seed extract, curry extract, elderberry concentrate, mangosteen concentrate, blackcurrant extract, blueberry extract, cowberry extract, spore cabbage concentrate, broccoli sprout concentrate, white pepper extract, apple extract, tomato concentrate, broccoli concentrate, carbomer concentrate, garlic concentrate, oregano concentrate, carrot concentrate, sweet cherry concentrate, raspberry concentrate, wild cherry concentrate, blackberry concentrate (0.1% each); 0.3% of resistant dextrin; silica 0.09%;

ergothioneine 0.01%.

Example 12

The plant source composition is prepared from the following components:

selecting one or more first plant components including quercetin, green tea extract, broccoli sprout extract, onion extract, apple extract, tomato extract, broccoli extract, carbomer extract, acerola extract, blueberry extract, sweet cherry extract, raspberry extract, spinach extract, cherokee rose extract, kale extract, blackberry extract, cowberry fruit extract, in an amount of 30-50% by weight; selecting one or more second plant components including olive extract, clove extract and resveratrol, wherein the content of the second plant components is 37-57% by weight; selecting one or more third plant components, including rosemary extract and grape seed extract, wherein the content by weight is 5% -20%; selecting one or more fourth plant components including turmeric extract, garlic extract, basil extract, oregano extract, cinnamon extract, carrot extract, elderberry extract, mangosteen extract, in an amount of 0.5% -2% by weight; selecting one or more fifth plant components including herba Ocimi extract, herba Origani extract, cortex Cinnamomi extract, and ramulus Sambuci Williamsii extract, wherein the content by weight is 0.5% -2%; the total weight of the composition was 100%.

Example 13 comparative example

The existing compound composition on the market:

acerola cherry berry extract 30%; quercetin 25%;

15% of a carbomer berry extract; an extract of phyllanthus emblica berries 15%;

10% of brazil berry juice powder; 5% of medlar berry extract.

Example 14 comparative example

The existing compound composition on the market:

apple fiber (237 mg) 32.6%; 30% of Brazil berry (215 mg);

mixed extracts (strawberry, blueberry, blackberry, raspberry, cranberry, medlar) (150 mg) 20%;

acerola cherry juice powder (110 mg) 15%; pomegranate juice powder (10 mg) 1.4%.

Example 16 Total antioxidant value (Total-ORAC) detection

The Total antioxidant value (Total-ORAC) is the sum of five indexes of antioxidant free radical (ORAC), hydroxyl free radical (HORAC), superoxide anion free radical (SORAC), nitrite peroxide (NORAC) and singlet oxygen free radical (SOAC). The total antioxidant value is obtained after five antioxidant free radical scavenging capacities of antioxidant free radical (ORAC), hydroxyl free radical (HORAC), superoxide anion free radical (SORAC), nitrite peroxide (NORAC) and singlet oxygen free radical (SOAC) are tested for the different compositions. The results are shown in Table 1.

TABLE 1 Total antioxidant value test results

	Total antioxidant value (mu moleTe/g)
		Example 2	21090
Example 3	20450
		Example 4	21652
Example 5	22809
		Example 7	23097
Example 8	28365
		Example 9	28675
Example 10	30873
		Example 11	37048
Example 13 (comparative example)	14045
		Example 14 (comparative example)	15600

The Total antioxidant value (Total-ORAC) of examples 6 and 12 is greater than 20,000 μmole TE/g, preferably greater than 30,000 μmole TE/g, more preferably greater than 35,000 μmole TE/g.

Example 17

The composition provided by the invention is compared with the commercial products in terms of smell, so that the composition is more acceptable in smell and taste, and the formula product is not easy to agglomerate and oxidize, so that the composition is more beneficial to preservation.

The plant source composition is easy to obtain, environment-friendly in source, safe in components and easy to be absorbed by intestinal tracts; the flavor is good, and the adaptability of a user is improved; has high antioxidant capacity, can effectively remove free radicals in human body, and can prevent or stop various diseases caused by excessive free radicals in human body. Further, the Total antioxidant value (Total-ORAC) of the present invention is greater than 20,000. Mu. Mole TE/g, which is far superior to existing commercial products, preferably greater than 30,000. Mu. Mole TE/g, more preferably greater than 35,000. Mu. Mole TE/g. The composition can produce synergistic effect by reasonable formula and proportion, and can exert stronger effect than single antioxidant by utilizing complementary effect of the structure-activity relationship. The flavor is more acceptable to consumers than other products in the market, and the formula product is not easy to agglomerate and oxidize.

The foregoing is only illustrative of the preferred embodiments of the invention and is not intended to be limiting, since various changes, modifications, substitutions and alterations can be made herein by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents.

Claims

1. A plant-derived composition comprising a first plant component, a second plant component, and a third plant component, the first plant component selected from one or more of the following: quercetin, green tea extract, broccoli sprout extract, onion extract, apple extract, tomato extract, broccoli extract, carbomer extract, acerola cherry extract, blueberry extract, sweet cherry extract, raspberry extract, spinach extract, cherokee rose extract, kale extract, blackberry extract, cowberry extract; the second plant component is selected from one or more of the following: olive extract, clove extract, resveratrol; the third plant component is selected from one or more of the following: rosemary extract, grape seed extract.

2. The plant source composition according to claim 1, wherein the plant source composition comprises 30% -50% by weight of the first plant component, 37% -57% of the second plant component, 5% -20% of the third plant component.

3. The plant source composition according to claim 1, further comprising 0.5% -2% by weight of a fourth plant component selected from one or more of the following: turmeric extract, garlic extract, basil extract, oregano extract, cinnamon extract, carrot extract, elderberry extract, mangosteen extract.

4. The plant source composition according to claim 1, further comprising from 0.5% to 2% by weight of a fifth plant component selected from one or more of the following: basil extract, oregano extract, cinnamon extract and elderberry extract.

5. The plant-derived composition of claim 1, further comprising fillers, glidants and additives.

6. The plant source composition according to claim 5, wherein the filler comprises one or more of the following: resistant dextrins, maltodextrins, lactose; the glidant comprises one or more of the following: silicon dioxide, magnesium stearate, talcum powder and tricalcium phosphate; the additive comprises one or more of the following: ergothioneine, L-ascorbic acid, sodium D-erythorbate, and tea polyphenols.

7. The plant-derived composition of claim 1, wherein the first plant component is quercetin and kale extract; the second plant component is olive extract; the third plant component is rosemary extract.

8. A method of using the plant-derived composition of any one of claims 1-7, comprising: the plant source composition is administered to a subject in need thereof in an amount of 0.1g to 200g per day.

9. The method of use of claim 8, wherein the botanical composition is formulated as a solid formulation or a liquid formulation.

10. Use of the plant-derived composition of any one of claims 1-7 for the preparation of a food, beverage, nutritional product, dietary supplement, cosmetic product.