CN116103095A - Magnetic particle chemiluminescent cleaning solution and preparation method thereof - Google Patents
Magnetic particle chemiluminescent cleaning solution and preparation method thereof Download PDFInfo
- Publication number
- CN116103095A CN116103095A CN202310364388.2A CN202310364388A CN116103095A CN 116103095 A CN116103095 A CN 116103095A CN 202310364388 A CN202310364388 A CN 202310364388A CN 116103095 A CN116103095 A CN 116103095A
- Authority
- CN
- China
- Prior art keywords
- surfactant
- mass fraction
- magnetic particle
- total mass
- cleaning solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004140 cleaning Methods 0.000 title claims abstract description 127
- 239000006249 magnetic particle Substances 0.000 title claims abstract description 99
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 239000004094 surface-active agent Substances 0.000 claims abstract description 80
- 239000000243 solution Substances 0.000 claims abstract description 77
- 239000002518 antifoaming agent Substances 0.000 claims abstract description 39
- 239000003093 cationic surfactant Substances 0.000 claims abstract description 38
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 35
- 239000002280 amphoteric surfactant Substances 0.000 claims abstract description 34
- 239000007853 buffer solution Substances 0.000 claims abstract description 30
- 239000003755 preservative agent Substances 0.000 claims abstract description 25
- 230000002335 preservative effect Effects 0.000 claims abstract description 25
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 11
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 11
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 11
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- MJJJJEUEZVGFLW-UHFFFAOYSA-N 2-dodecyl-2-sulfobutanedioic acid Chemical compound CCCCCCCCCCCCC(S(O)(=O)=O)(C(O)=O)CC(O)=O MJJJJEUEZVGFLW-UHFFFAOYSA-N 0.000 claims abstract description 5
- TVACALAUIQMRDF-UHFFFAOYSA-N dodecyl dihydrogen phosphate Chemical compound CCCCCCCCCCCCOP(O)(O)=O TVACALAUIQMRDF-UHFFFAOYSA-N 0.000 claims abstract description 5
- 150000001413 amino acids Chemical class 0.000 claims abstract description 4
- 239000013530 defoamer Substances 0.000 claims description 39
- 239000007983 Tris buffer Substances 0.000 claims description 28
- LNOPIUAQISRISI-UHFFFAOYSA-N n'-hydroxy-2-propan-2-ylsulfonylethanimidamide Chemical compound CC(C)S(=O)(=O)CC(N)=NO LNOPIUAQISRISI-UHFFFAOYSA-N 0.000 claims description 27
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 21
- 239000004721 Polyphenylene oxide Substances 0.000 claims description 19
- 229920000570 polyether Polymers 0.000 claims description 19
- 229920002545 silicone oil Polymers 0.000 claims description 18
- 150000003841 chloride salts Chemical class 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 5
- 239000012498 ultrapure water Substances 0.000 claims description 5
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 229920001296 polysiloxane Polymers 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 48
- 230000000694 effects Effects 0.000 abstract description 45
- 238000001514 detection method Methods 0.000 abstract description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 abstract 1
- 238000005406 washing Methods 0.000 description 28
- 230000000052 comparative effect Effects 0.000 description 27
- 108010048233 Procalcitonin Proteins 0.000 description 20
- CWCXERYKLSEGEZ-KDKHKZEGSA-N procalcitonin Chemical compound C([C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)NCC(O)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H]1NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@@H](N)CSSC1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 CWCXERYKLSEGEZ-KDKHKZEGSA-N 0.000 description 19
- 239000003154 D dimer Substances 0.000 description 18
- 108010052295 fibrin fragment D Proteins 0.000 description 18
- 102400001263 NT-proBNP Human genes 0.000 description 12
- 238000003149 assay kit Methods 0.000 description 12
- 108010008064 pro-brain natriuretic peptide (1-76) Proteins 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 239000006260 foam Substances 0.000 description 9
- 239000003550 marker Substances 0.000 description 9
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 8
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 101800000407 Brain natriuretic peptide 32 Proteins 0.000 description 7
- 102400000667 Brain natriuretic peptide 32 Human genes 0.000 description 7
- 101800002247 Brain natriuretic peptide 45 Proteins 0.000 description 7
- 238000004020 luminiscence type Methods 0.000 description 7
- HPNRHPKXQZSDFX-OAQDCNSJSA-N nesiritide Chemical group C([C@H]1C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CSSC[C@@H](C(=O)N1)NC(=O)CNC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CO)C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1N=CNC=1)C(O)=O)=O)[C@@H](C)CC)C1=CC=CC=C1 HPNRHPKXQZSDFX-OAQDCNSJSA-N 0.000 description 7
- 239000002243 precursor Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 5
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 231100000956 nontoxicity Toxicity 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000005281 excited state Effects 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000009776 industrial production Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical group Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 229920000877 Melamine resin Polymers 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 230000005283 ground state Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 206010057362 Underdose Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002563 ionic surfactant Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000000504 luminescence detection Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
- C11D1/94—Mixtures with anionic, cationic or non-ionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/042—Acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/26—Organic compounds containing nitrogen
- C11D3/30—Amines; Substituted amines ; Quaternized amines
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/34—Organic compounds containing sulfur
- C11D3/349—Organic compounds containing sulfur additionally containing nitrogen atoms, e.g. nitro, nitroso, amino, imino, nitrilo, nitrile groups containing compounds or their derivatives or thio urea
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/02—Anionic compounds
- C11D1/12—Sulfonic acids or sulfuric acid esters; Salts thereof
- C11D1/123—Sulfonic acids or sulfuric acid esters; Salts thereof derived from carboxylic acids, e.g. sulfosuccinates
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/02—Anionic compounds
- C11D1/34—Derivatives of acids of phosphorus
- C11D1/345—Phosphates or phosphites
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/74—Carboxylates or sulfonates esters of polyoxyalkylene glycols
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Inorganic Chemistry (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The application discloses a magnetic particle chemiluminescence cleaning solution and a preparation method thereof, wherein the magnetic particle chemiluminescence cleaning solution comprises a surfactant, a buffer solution, a preservative, chloride, a defoaming agent and water; wherein the surfactant comprises cationic surfactant, nonionic surfactant and amphoteric surfactant; the cationic surfactant is one of lauryl sulfosuccinic acid monoester disodium and monolauryl phosphate; the nonionic surfactant comprises tween-20; the amphoteric surfactant includes an amino acid type surfactant. The magnetic particle chemiluminescent cleaning liquid has the advantages of good cleaning effect, good stability and low cost, and therefore detection accuracy of a sample to be detected is improved. Therefore, has good practical application value.
Description
Technical Field
The application relates to the technical field of chemiluminescence analysis, in particular to a magnetic particle chemiluminescence cleaning solution and a preparation method thereof.
Background
The magnetic particle chemiluminescence immunoassay technology combines a magnetic particle carrier technology and a chemiluminescence immunoassay technology, so that the measurement result is more accurate and more stable. Wherein the magnetic particle chemiluminescence method comprises the following steps: adding a sample to be tested, incubating, washing, adding a substrate solution, and reading a luminescence value. Wherein, the cleaning to remove other substances except the object to be tested, and the reduction of the background value of luminescence detection are an important step for determining the test result. The main purpose of the washing is to remove unbound immunoreactants and free enzyme labels, prevent the antigen and antibody from being continuously bound, and reduce the influence of non-specific interfering substances on the detection result. The cleaning solution not only can provide a proper pH value environment for immune reaction, but also can remove unbound immune reactant in the reaction process, stop antigen and antibody from continuously binding, and the surfactant in the cleaning solution can effectively separate bound immune reactant and nonspecific interfering substances.
At present, the types of chemiluminescent cleaning liquid are huge, but most of the cleaning liquid is original cleaning liquid, the price is high, the detection cost is greatly increased, and the cleaning liquid used by the chemiluminescent measuring instruments of different manufacturers and different types cannot be commonly used. Some cleaning solutions have poor cleaning effect, so that the background value of luminescence is high; some cleaning solutions have complex components, depend on chemical components imported abroad, and have higher cost.
Disclosure of Invention
In order to solve at least one of the technical problems, a magnetic particle chemiluminescent cleaning solution which is accurate and stable in cleaning effect, low in cost and capable of being used universally is developed.
In a first aspect, the present application provides a magnetic particle chemiluminescent cleaning solution comprising a surfactant, a buffer, a preservative, a chloride salt, an antifoaming agent, and water;
wherein the mass fraction of the surfactant is 4.3% -6.8%;
the buffer solution comprises Tris buffer solution and sulfamic acid, and the total mass fraction is 0.5-1.5%;
the preservative comprises Proclin-300, and the total mass fraction is 0.05-0.1%;
the chloride salt comprises NaCl, and the total mass fraction is 0.7-1.5%;
the total mass fraction of the defoaming agent is 0.8-1.5%;
the surfactant comprises a cationic surfactant, a nonionic surfactant and an amphoteric surfactant;
the cationic surfactant is one of lauryl sulfosuccinic acid monoester disodium and monolauryl phosphate;
the nonionic surfactant comprises tween-20; the amphoteric surfactant includes an amino acid type surfactant.
Through adopting above-mentioned technical scheme, first aspect, this application has solved the washing liquid cleaning effect poor, problem that can not be general, has solved the influence that the washing liquid produced luminous background value simultaneously to make luminous value more accurate, and this application the composition in a magnetic particle chemiluminescence washing liquid is simple easily obtained, and is nontoxic, convenient transportation, but industrial production. The mechanism is that the lauryl sulfosuccinic acid monoester disodium or monolauryl phosphate belongs to a cationic surfactant, and has strong detergency, no irritation, stability and small temperature change; meanwhile, the tween-20 belongs to a nonionic surfactant, and is favorable for reducing the nonspecific binding of antibody antigens, so that the experimental result is more accurate; in a second aspect, the magnetic particle chemiluminescent cleaning solution can achieve a more stable cleaning effect. The mechanism is that in the prior art, a common buffer system is Tris-HCl, and HCl has higher corrosiveness and has risks in use. In order to solve the problems of a Tris-HCL buffer system, a Tris-sulfamic acid buffer system consisting of a Tris buffer solution and sulfamic acid is used in the application, so that the solution is kept at a proper pH value, and the immune complex is kept stable; in the third aspect, the magnetic particle chemiluminescent cleaning solution can achieve good antibacterial effect and better stability. The mechanism is that the Proclin-300 has broad-spectrum antibacterial capability on bacteria, fungi and saccharomycetes. Can rapidly eliminate the activity of microorganisms, eliminate the biological film and inhibit the reformation of the biological film even at low concentration. Meanwhile, proclin-300 can be compatible with a wide range of components of a cleaning solution formula such as a surfactant, has excellent chemical stability and has good compatibility with most raw materials or formulas. And the Proclin-300 product is colorless, and does not influence the change of the absorbance of the indicating substance in the reaction system. At the recommended temperature, the stability of unopened products of the magnetic particle chemiluminescent cleaning liquid added with Proclin-300 can reach more than one year; and has good biodegradability and no pollution to the environment. The stability of the magnetic particle chemiluminescent cleaning liquid is further improved by adding NaCl. The mechanism is that the NaCl described in this application provides the proper ion concentration to keep the immune complex stable.
Optionally, the mass fraction of the surfactant is 5.3% -6.5%;
the total mass fraction of the buffer solution is 0.5-1.5%;
the total mass fraction of the preservative is 0.05-0.1%;
the total mass fraction of the chloride salt is 0.7-1.5%;
the total mass fraction of the defoaming agent is 0.8-1.5%.
Further optionally, the surfactant has a mass fraction of 6.2%;
the total mass fraction of the buffer solution is 1.2%;
the total mass fraction of the preservative is 0.1%;
the total mass fraction of the chloride salt is 1.3%;
the total mass fraction of the defoamer is 1.2%.
Optionally, the mass fraction ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 2:3:3-5.
Further optionally, the mass fraction ratio between the cationic surfactant, the nonionic surfactant and the amphoteric surfactant is 2:3:5.
Optionally, the mass fraction ratio of the Tris buffer solution to the sulfamic acid is 12:7-10.
Further alternatively, the mass fraction ratio of Tris buffer to sulfamic acid is 3:2.
Optionally, the defoamer comprises a silicone oil type defoamer and a polyether type defoamer.
Through adopting above-mentioned technical scheme, this application a magnetic particle chemiluminescence washing liquid can realize better stability and cleaning performance. The mechanism is that the silicone oil defoamer and the polyether defoamer can solve the problem of foam generated in the cleaning process, and avoid the influence of the foam on the experimental accuracy, thereby improving the detection accuracy of the object to be cleaned. Wherein, the silicone oil defoamer has strong chemical stability, no physiological toxicity and difficult reaction with other substances. The surface tension of the foam is lower than that of foaming liquid such as 73mN/m water, 35-40 mN/m vegetable oil and the like, and the surface tension is generally 16-21 mN/m. The foam removing agent has small acting force among foam removing molecules, is favorable for adsorbing or displacing foam, and rapidly diffuses in foaming liquid to achieve the purpose of rapid foam removing, thereby avoiding the influence of the foam on experiments. At the same time, it can be used in a wide temperature range and is little affected by temperature.
The polyether defoamer has the characteristics of no toxicity, no smell, no stimulation, easy dispersion in water and the like, and is not only the defoamer but also an ionic surfactant. The polyether type defoamer has excellent defoaming and foam inhibiting functions. Therefore, the application adopts the silicone oil defoamer and the polyether defoamer simultaneously, so that the magnetic particle chemiluminescent cleaning liquid has better cleaning effect and is more stable.
Optionally, the mass fraction ratio of the silicone oil type defoamer to the polyether type defoamer is 1:1.
In a second aspect, the present application provides a method for preparing a magnetic particle chemiluminescent cleaning solution, comprising the steps of:
preparing a buffer solution with the pH value of 7.4+/-0.2, sequentially adding chloride salt, a surfactant, a preservative and a defoaming agent into the buffer solution, uniformly mixing, and adding ultrapure water to a constant volume of 1L to obtain the magnetic particle chemiluminescent cleaning solution.
Through adopting above-mentioned technical scheme, the application a magnetic particle chemiluminescence washing liquid can realize that preparation operation is simple and convenient, effect with low costs. The mechanism is that all raw materials in the magnetic particle chemiluminescent cleaning liquid are nontoxic, cheap and easy to obtain. Meanwhile, the magnetic particle chemiluminescent cleaning liquid has the characteristics of high stability, good cleaning effect and low cost, and can be used for industrial production.
In summary, the present invention includes at least one of the following beneficial technical effects:
1. the magnetic particle chemiluminescent cleaning solution has the characteristics of stable components, no toxicity and safety.
2. The magnetic particle chemiluminescent cleaning solution has the characteristics of cheap and easily available raw materials and the like, and can be industrially produced.
3. The magnetic particle chemiluminescent cleaning liquid has the characteristics of high stability, good cleaning effect and the like.
4. The magnetic particle chemiluminescent cleaning solution has good antibacterial effect.
5. The magnetic particle chemiluminescent cleaning solution has good biodegradability and can not pollute the environment.
Detailed Description
The present invention will be described in further detail with reference to examples.
The inventors found that there is a large error between the detection result and the actual result when conducting a luminescent immunoassay in response to binding of an antigen antibody. The inventor has found through many experiments that the chemiluminescent washing liquid used in the experimental process is a main cause of low detection accuracy. The inventor has replaced several different chemiluminescent cleaning solutions, but these chemiluminescent cleaning solutions have advantages and disadvantages, and some chemiluminescent cleaning solutions have good cleaning effect but high cost; however, some chemiluminescent cleaning solutions are cheap, but have the problem of poor cleaning effect. The inventor solves the problems existing in the chemiluminescent cleaning liquid in the prior art, and obtains a formula of the chemiluminescent cleaning liquid with good cleaning effect, stable components, no toxicity, safety, low cost and little influence on experimental background value through researches and experiments, wherein the formula of the chemiluminescent cleaning liquid is as follows:
in a first aspect, the present application provides a magnetic particle chemiluminescent cleaning solution comprising a surfactant, a buffer, a preservative, a chloride salt, an antifoaming agent, and water;
wherein the mass fraction of the surfactant is 4.3% -6.8%;
the buffer solution comprises Tris buffer solution and sulfamic acid, and the total mass fraction is 0.5-1.5%;
the preservative comprises Proclin-300, and the total mass fraction is 0.05-0.1%;
the chloride salt comprises NaCl, and the total mass fraction is 0.7-1.5%;
the total mass fraction of the defoaming agent is 0.8-1.5%;
the surfactant comprises a cationic surfactant, a nonionic surfactant and an amphoteric surfactant;
the cationic surfactant is one of lauryl sulfosuccinic acid monoester disodium and monolauryl phosphate;
the nonionic surfactant comprises tween-20; the amphoteric surfactant includes an amino acid type surfactant.
The inventor finds that the larger the mass fraction of the surfactant is, the better the detection is when the magnetic particle chemiluminescent cleaning liquid is adopted, and the inventor obtains through a plurality of experiments that when the mass fraction of the surfactant exceeds 6.2%, the cleaning effect of the magnetic particle chemiluminescent cleaning liquid provided by the application is in a decreasing trend. The inventors speculate that when the mass fraction of the surfactant exceeds 6.2%, the defoaming agent described herein cannot perform better defoaming on the surfactant, thereby leading to a decrease in the cleaning effect of the magnetic particle chemiluminescent cleaning liquid provided herein.
On the basis, the inventor provides a preparation method of the magnetic particle chemiluminescent cleaning solution, which comprises the following steps:
preparing a buffer solution with the pH value of 7.4+/-0.2, sequentially adding chloride salt, a surfactant, a preservative and a defoaming agent into the buffer solution, uniformly mixing, and adding ultrapure water to a constant volume of 1L to obtain the magnetic particle chemiluminescent cleaning solution.
The magnetic particle chemiluminescent cleaning liquid has the characteristics of stable components, no toxicity, safety, low cost, good cleaning effect and the like. Meanwhile, the preparation method of the magnetic particle chemiluminescent cleaning liquid is simple and convenient, raw materials are easy to obtain, and the magnetic particle chemiluminescent cleaning liquid can be used for industrial production.
Examples 1-7 preparation of a magnetic particle chemiluminescent cleaning solution
The volume of a magnetic particle chemiluminescent rinse prepared in the examples herein below was 1L. The components of the magnetic particle chemiluminescent cleaning solution can be purchased from the market.
Example 1
This example prepares a magnetic particle chemiluminescent cleaning solution comprising the ingredients shown in table 1. The preparation method of the magnetic particle chemiluminescent cleaning liquid comprises the following steps:
firstly, preparing a buffer solution with the pH value of 7.4, sequentially adding a chloride salt, a surfactant, a preservative and a defoaming agent into the buffer solution, fully and uniformly mixing by using a magnetic stirrer, secondly, adding ultrapure water to fix the volume to 1L, and finally, regulating the pH value to 7.4+/-0.2 to obtain the magnetic particle chemiluminescent cleaning solution.
In this embodiment, the total mass fraction of the surfactant is 4.3%;
the surfactant comprises a cationic surfactant, a nonionic surfactant and an amphoteric surfactant; the weight ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 1:1:3;
wherein the cationic surfactant is lauryl sulfosuccinic monoester disodium; the nonionic surfactant is Tween-20; the amphoteric surfactant is N-alkyl aspartic acid-beta-alkyl ester;
the total mass fraction of the buffer solution is 0.5%, and the buffer solution comprises Tris buffer solution and sulfamic acid; the ratio of the dosage of the Tris buffer solution to the dosage of sulfamic acid is 2:3;
the total mass fraction of the preservative is 0.05%, and the preservative is Proclin-300;
the total mass fraction of NaCl is 0.7%;
the total mass fraction of the defoaming agent is 0.8%, and the defoaming agent is trialkyl melamine.
Example 2
The total mass fraction of the surfactant in this example was 4.5%; the weight ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 4:2:7;
wherein the cationic surfactant is lauryl sulfosuccinic monoester disodium; the nonionic surfactant is Tween-20; the amphoteric surfactant is N-alkyl aspartic acid-beta-alkyl ester;
the total mass fraction of the buffer solution is 0.8%, and the buffer solution comprises Tris buffer solution and sulfamic acid; the ratio of the dosage of the Tris buffer solution to the dosage of sulfamic acid is 3:4;
the total mass fraction of the preservative is 0.07%, and the preservative is Proclin-300;
the total mass fraction of NaCl is 0.9%;
the total mass fraction of the defoaming agent is 1.0%, and the defoaming agent is a polyether type defoaming agent.
Example 3
The total mass fraction of the surfactant in this example was 5.0%; the weight ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 7:9:13;
wherein the cationic surfactant is lauryl sulfosuccinic monoester disodium; the nonionic surfactant is Tween-20; the amphoteric surfactant is N-alkyl aspartic acid-beta-alkyl ester;
the total mass fraction of the buffer solution is 1.0%, and the buffer solution comprises Tris buffer solution and sulfamic acid; the ratio of the dosage of the Tris buffer solution to the dosage of sulfamic acid is 4:3;
the total mass fraction of the preservative is 0.09%, and the preservative is Proclin-300;
the total mass fraction of NaCl is 1.0%;
the total mass fraction of the defoaming agent is 1.2%, and the defoaming agent is a silicone oil type defoaming agent.
Example 4
The total mass fraction of the surfactant in this example was 6.0%; the weight ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 2:3:6;
wherein the cationic surfactant is lauryl sulfosuccinic monoester disodium; the nonionic surfactant is Tween-20; the amphoteric surfactant is Na-L-lysine;
the total mass fraction of the buffer solution is 1.5%, and the buffer solution comprises Tris buffer solution and sulfamic acid; the ratio of the dosage of the Tris buffer solution to the dosage of sulfamic acid is 1:1;
the total mass fraction of the preservative is 0.1%, and the preservative is Proclin-300;
the total mass fraction of NaCl is 1.2%;
the total mass fraction of the defoaming agent is 1.5%, the defoaming agent is a polyether type defoaming agent and a silicone oil type defoaming agent, and the mass fraction ratio relationship between the polyether type defoaming agent and the silicone oil type defoaming agent is 2:3.
Example 5
The total mass fraction of the surfactant in this example was 6.8%; the weight ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 2:3:6;
wherein the cationic surfactant is lauryl sulfosuccinic monoester disodium; the nonionic surfactant is Tween-20; the amphoteric surfactant is Na-L-lysine;
the total mass fraction of the buffer solution is 1.5%, and the buffer solution comprises Tris buffer solution and sulfamic acid; the ratio of the dosage of the Tris buffer solution to the dosage of sulfamic acid is 1:1;
the total mass fraction of the preservative is 0.1%, and the preservative is Proclin-300;
the total mass fraction of NaCl is 1.5%;
the total mass fraction of the defoaming agent is 1.5%, the defoaming agent is a polyether type defoaming agent and a silicone oil type defoaming agent, and the ratio relationship between the polyether type defoaming agent and the silicone oil type defoaming agent is 1:3.
Example 6
This example differs from example 1 in that the weight ratio between cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:2:5.
Example 7
The difference between this embodiment and embodiment 1 is that the defoamer in this embodiment is a polyether defoamer and a silicone oil defoamer, and the mass ratio of the polyether defoamer to the silicone oil defoamer is 4:5.
Comparative example 1
The present comparative example differs from example 1 in that in the present comparative example, the total mass fraction of the surfactant is 4.0%.
Comparative example 2
The present comparative example differs from example 1 in that in the present comparative example, no cationic surfactant was added.
Comparative example 3
The present comparative example differs from example 1 in that no antifoaming agent was added in the present comparative example.
Comparative example 4
The difference between this comparative example and example 1 is that this comparative example produces a prior art cleaning solution having the following formulation:
tris:154.4g; nacl:149g; tween 20:56.2g;37% HCl:94.4mL; and (5) using ultrapure water to fix the volume to lL.
Influence experiment of background value and linear width of luminescence value
The magnetic particle chemiluminescent cleaning solutions prepared in examples 1-7 and comparative examples 1-4 were used to clean NT-proBNP assay kits, PCT assay kits, and D-Dimer assay kits, and the influence of the magnetic particle chemiluminescent cleaning solutions on the background value of the luminescent value and the linear width was verified.
The NT-proBNP assay kit, the PCT assay kit and the D-Dimer assay kit comprise magnetic particles coated by monoclonal antibodies, monoclonal antibodies and alkaline phosphatase markers;
specific:
the NT-proBNP assay kit comprises magnetic particles coated with an N-terminal brain natriuretic peptide precursor monoclonal antibody, an N-terminal brain natriuretic peptide precursor monoclonal antibody and an alkaline phosphatase marker;
the PCT assay kit comprises procalcitonin monoclonal antibody coated magnetic particles, procalcitonin monoclonal antibody and alkaline phosphatase marker;
the D-Dimer assay kit comprises magnetic particles coated with a D-Dimer monoclonal antibody, the D-Dimer monoclonal antibody and an alkaline phosphatase marker.
The specific detection steps and detection principles are as follows:
the magnetic particle chemiluminescence cleaning solutions prepared in examples 1 to 7 and comparative examples 1 to 4 are sequentially used for cleaning an N-terminal brain natriuretic peptide precursor (NT-proBNP) measuring kit, a Procalcitonin (PCT) measuring kit and a D-Dimer (D-Dimer) measuring kit respectively, and the cleaning methods of the NT-proBNP measuring kit, the PCT measuring kit and the D-Dimer measuring kit are the same in each experiment.
Carrying out 10 tests on 9 samples to be tested in total in each test, wherein the concentrations of NT-proBNP in samples A, B and C are respectively 0, 130 and 30000pg/mL; samples D, E and F had PCT concentrations of 0, 0.25 and 100ng/mL, respectively; sample G, H and D-Dimer concentrations in I were 0, 0.5 and 5. Mu.g/mL, respectively.
The specific cleaning method comprises the following steps:
s1, mixing a sample to be detected, magnetic particles coated by an N-terminal brain natriuretic peptide precursor monoclonal antibody, the N-terminal brain natriuretic peptide precursor monoclonal antibody and an alkaline phosphatase marker to form a magnetic particle-coated antibody-sample to be detected-abzyme marker immune complex of NT-proBNP, and incubating for reaction;
mixing a sample to be tested, magnetic particles coated by procalcitonin monoclonal antibody, procalcitonin monoclonal antibody and alkaline phosphatase marker to form PCT 'magnetic particles-coated antibody-sample to be tested-antibody enzyme marker' immune complex, and incubating for reaction;
mixing a sample to be tested, magnetic particles coated by a D-Dimer monoclonal antibody and the D-Dimer monoclonal antibody with an alkaline phosphatase marker to form a magnetic particle-coated antibody-sample to be tested-antibody enzyme marker immune complex of the D-Dimer, and incubating for reaction;
s2, sequentially adopting the magnetic particle chemiluminescence cleaning solutions prepared in the examples 1-7 and the comparative examples 1-4 to clean immune complexes prepared in the step S1 respectively, and washing away unbound components in the step S1;
s3, adding chemiluminescent substrate liquid into the immune complex in the step S2, and performing catalytic cleavage on the substrate liquid by alkaline phosphatase to form an unstable excited state intermediate; the excited state intermediate releases photons when returning to the ground state;
s4, detecting the luminous intensity of photons released when the excited state intermediate in the step S3 returns to the ground state by using a luminometer;
s5, analyzing the luminous intensity of the step S4, so as to respectively calculate the concentrations of NT-proBNP, PCT and D-Dimer in the sample to be detected;
s6, analyzing the concentration of the NT-proBNP, PCT and D-Dimer obtained in the step S5, and calculating the cleaning effect of the magnetic particle chemiluminescence cleaning solution.
The luminescence values of samples A, B, C, D, E, F, G, H and I are shown in table 1;
table 1 sample A, B, C, D, E, F, G, H and luminescence value summary table of I
Analysis of results:
the data in Table 1 above are CV values of each sample obtained by washing N-terminal brain natriuretic peptide precursor (abbreviated as NT-proBNP) measurement kit, procalcitonin (abbreviated as PCT) measurement kit and D-Dimer (abbreviated as D-Dimer) measurement kit with magnetic particle chemiluminescent washing liquid, and detecting the luminescent value of each sample.
After comparing each CV value, the magnetic particle chemiluminescence cleaning solution prepared in the examples 1-7 is used for cleaning the object to be cleaned, and the variation coefficient (CV value) is smaller than 10%, so that the obtained magnetic particle chemiluminescence cleaning solution prepared in the examples 1-7 meets the clinical examination requirement.
Among them, the magnetic particle chemiluminescent washing liquid prepared in example 4 had the lowest coefficient of variation (CV value), and thus it was found that the magnetic particle chemiluminescent washing liquid prepared in example 4 had the best washing stability.
Comparative example 1 differs from example 1 in that the total mass fraction of the surfactant described in comparative example 1 is 4.0%; as can be seen from the data in Table 1, the magnetic particle chemiluminescent cleaning solution prepared in comparative example 1 was used to clean the object to be cleaned, and the coefficient of variation (CV value) was greater than 10%, which did not meet the clinical examination requirements. Therefore, the total mass fraction of the surfactant is 4.3% or more, which is helpful for the cleaning effect of the magnetic particle chemiluminescence cleaning solution to meet the clinical examination requirement.
Comparative example 2 differs from example 1 in that no cationic surfactant was added in this comparative example. As can be seen from the data in table 1, the magnetic particle chemiluminescent washing liquid prepared in comparative example 2 was used to wash the object to be washed, and the coefficient of variation (CV value) of a part of the sample was greater than 10% and the coefficient of variation (CV value) of another part of the sample was less than 10%, so that it was found that the surfactant including the cationic surfactant contributed to the washing effect of the magnetic particle chemiluminescent washing liquid to meet the clinical examination requirements when the magnetic particle chemiluminescent washing liquid was prepared.
Comparative example 3 differs from example 1 in that no antifoaming agent was added in this comparative example. As can be seen from the data in Table 1, the magnetic particle chemiluminescent washing liquid prepared in comparative example 3 was used to wash the object to be washed, wherein the coefficient of variation (CV value) of a part of the sample was greater than 10%, and the coefficient of variation (CV value) of another part of the sample was less than 10%, so that it was found that the defoaming agent was added when the magnetic particle chemiluminescent washing liquid was prepared, which was favorable for the washing effect of the magnetic particle chemiluminescent washing liquid to meet the clinical examination requirements.
Comparative example 4 differs from example 1 in that comparative example 4 employs a prior art magnetic particle chemiluminescent washing liquid. Comparison of the data of example 4 with that of example 1 shows that the CV value of the chemiluminescent washing liquid for magnetic particles prepared in example 1 is lower than that of comparative example 4.
According to the CV values of examples 1 to 7, it can be seen that the concentration of the surfactant has a great influence on the cleaning effect of the magnetic particle chemiluminescent cleaning liquid, and the effect is optimal when the concentration of the surfactant is about 6%, and the cleaning effect tends to decrease when the concentration is lower than or higher than 6%. The inventors speculate that the reason for the above trend is that the surfactant concentration is too low, which may result in poor cleaning effect due to insufficient dosage; the too high concentration of the surfactant can cause the magnetic particle chemiluminescent cleaning liquid to be difficult to wash and clean due to too large dosage, and residues exist, so that the cleaned object can remain in the system, and the cleaning effect is further affected.
In order to further optimize the amount and the ratio of the surfactant, examples 8 to 26 of the present application are as follows.
Examples 8 to 26 were tested for NT-proBNP and the test samples were A, B, C, respectively.
Example 8
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.0%. The remaining conditions were identical to those of example 2.
Example 9
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.1%. The remaining conditions were identical to those of example 2.
Example 10
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.2%. The remaining conditions were identical to those of example 2.
Example 11
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.3%. The remaining conditions were identical to those of example 2.
Example 12
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.4%. The remaining conditions were identical to those of example 2.
Example 13
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.5%. The remaining conditions were identical to those of example 2.
Example 14
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.6%. The remaining conditions were identical to those of example 2.
Example 15
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.7%. The remaining conditions were identical to those of example 2.
Example 16
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.8%. The remaining conditions were identical to those of example 2.
Example 17
This example differs from example 2 in that the total mass fraction of surfactant in this example is 5.9%. The remaining conditions were identical to those of example 2.
Example 18
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.0%. The remaining conditions were identical to those of example 2.
Example 19
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.1%. The remaining conditions were identical to those of example 2.
Example 20
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.2%. The remaining conditions were identical to those of example 2.
Example 21
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.3%. The remaining conditions were identical to those of example 2.
Example 22
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.4%. The remaining conditions were identical to those of example 2.
Example 23
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.5%. The remaining conditions were identical to those of example 2.
Example 24
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.6%. The remaining conditions were identical to those of example 2.
Example 25
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.7%. The remaining conditions were identical to those of example 2.
Example 26
This example differs from example 2 in that the total mass fraction of surfactant in this example is 6.8%. The remaining conditions were identical to those of example 2.
The test data of examples 8 to 26 are shown in Table 2;
TABLE 2CV values summary of examples 8-26 samples A, B, C
According to the CV values of examples 8 to 26, it can be seen that the concentration of the surfactant has a great influence on the cleaning effect of the magnetic particle chemiluminescent cleaning liquid, and the effect is optimal when the concentration of the surfactant is 6.2%, and the cleaning effect tends to decrease when the concentration is lower than or higher than 6.2%.
As can be seen from CV values of examples 1 to 7, the effect was optimal when the concentration of the surfactant was about 6%, and example 4 was identical to example 18 in that the concentration of the surfactant was 6.0% in both examples 4 and 18. Comparison of the results shows that the cleaning effect of example 4 is more stable than that of example 18, as the CV value is different, although the surfactant concentration is the same in example 4 and example 18. The inventors speculate that the reason for the above trend is that the selection of the ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant may affect the cleaning effect.
In order to further optimize the amount and the ratio of the surfactant, examples 27 to 30 of the present application are as follows.
Example 27
This example differs from example 4 in that the total mass fraction of surfactant in this example is 6.1%. The remaining conditions were identical to those of example 4.
Example 28
This example differs from example 4 in that the total mass fraction of surfactant in this example is 6.2%. The remaining conditions were identical to those of example 4.
Example 29
This example differs from example 4 in that the total mass fraction of surfactant in this example is 6.3%. The remaining conditions were identical to those of example 4.
Example 30
This example differs from example 4 in that the total mass fraction of surfactant in this example is 6.4%. The remaining conditions were identical to those of example 4.
The test results of examples 27 to 30 are shown in Table 3;
TABLE 3 CV values summary table of examples 27 to 30 samples A, B, C
According to the CV values of examples 27 to 30, it can be seen that the concentration of the surfactant has a great influence on the cleaning effect of the magnetic particle chemiluminescent cleaning liquid, and the effect is optimal when the concentration of the surfactant is 6.2%, and the cleaning effect tends to decrease when the concentration is lower than or higher than 6.2%. The results obtained from the CV values of examples 8 to 26 were further confirmed.
The CV values of examples 27 to 30 are sequentially compared with examples 19 to 22, and it is understood that the CV value of example 27 is smaller than the CV value of example 19 when the surfactant mass fraction is the same. The CV value of example 28 is less than the CV value of example 20. The CV value of example 29 is smaller than that of example 21. The CV value of example 30 is less than the CV value of example 22. From these results, it can be seen that the ratio by weight between the cationic surfactant, the nonionic surfactant, and the amphoteric surfactant affects the cleaning effect when the mass fraction of the surfactant is the same. The conclusions drawn from examples 8-26 were further verified.
Examples 31 to 35 below
Based on example 28, the mass fraction ratio between cationic surfactant, nonionic surfactant, and amphoteric surfactant was further optimized.
Example 31
The difference between this example and example 28 is that the mass fraction ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:3:3.
Example 32
The difference between this example and example 28 is that the mass fraction ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:3:4.
Example 33
The difference between this example and example 28 is that the mass fraction ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:3:5.
Example 34
The difference between this example and example 28 is that the mass fraction ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:3:7.
Example 35
The difference between this example and example 28 is that the mass fraction ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant in this example is 2:3:8.
The test data of examples 31 to 35 are shown in Table 4;
TABLE 4 CV values summary of examples 31 to 35 samples A, B, C
According to the CV values of examples 31 to 35, it can be seen that when the mass fraction ratio of the cationic surfactant, the nonionic surfactant, and the amphoteric surfactant is 2:3:3 to 5, the CV values are all less than 7%. And when the mass fraction ratio of the cationic surfactant to the nonionic surfactant to the amphoteric surfactant is 2:3:5, the CV value is the lowest, and the cleaning effect is the best.
The inventors have adjusted the ratio of the amounts of Tris buffer and sulfamic acid based on example 33 in order to further optimize the amounts and ratios of the components.
The following are examples 36 to 41 of the present application.
Example 36
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 2:1.
Example 37
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 12:7.
Example 38
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 3:2.
Example 39
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 4:3.
Example 40
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 6:5.
Example 41
This example differs from example 33 in that in this example the ratio of Tris buffer to sulfamic acid is 12:11.
The test data of examples 36 to 41 are shown in Table 5;
TABLE 5 CV values summary table of examples 36 to 41 samples A, B, C
According to CV values of examples 36 to 41, it can be seen that the washing effect is significantly better than other examples when the ratio of Tris buffer to sulfamic acid is 12:7 to 10. The washing effect is best when the ratio of Tris buffer to sulfamic acid is 3:2.
The inventors have adjusted the selection of defoamers based on example 38 in order to further optimize the amounts and proportions of the components.
Example 42
This example differs from example 38 in that in this example, the defoamer is a polyether defoamer.
Example 43
The difference between this embodiment and embodiment 38 is that in this embodiment, the defoaming agent is a silicone oil type defoaming agent.
Example 44
This example differs from example 38 in that in this example the defoamer is a trialkyl melamine.
The detection results of examples 42 to 44 are shown in Table 6;
table 6 CV value summary table of examples 42 to 44 samples A, B, C
According to CV values of examples 38 and 42 to 44, it can be seen that the cleaning effect is improved when the defoamer is a polyether defoamer or a silicone oil defoamer.
The inventors further adjusted the proportions of the polyether type defoamer and the silicone oil type defoamer based on example 38 in order to further optimize the amounts and proportions of the components.
Example 45
This example differs from example 38 in that the ratio of polyether type defoamer to silicone oil type defoamer in this example is 1:1.
Example 46
This example differs from example 38 in that the ratio of polyether-type defoamer to silicone-type defoamer in this example is 3:2.
The test results of examples 45 to 46 are shown in Table 7;
TABLE 7 CV values summary table of examples 45-46 samples A, B, C
According to CV values of examples 38 and 45-46, it can be seen that the cleaning effect is improved when the mass fraction ratio relationship between the polyether type defoamer and the silicone oil type defoamer is 1:1.
Cleaning effect verification experiment
Examples 47 to 49 below first clean N-terminal brain natriuretic peptide precursor (abbreviated as NT-proBNP) assay kit, procalcitonin (abbreviated as PCT) assay kit and D-Dimer (abbreviated as D-Dimer) assay kit, then test the luminescence values of each sample 10 times, and detect the mean value by the luminescence values, and finally calculate the ratio between B/A, C/B, E/D, F/E, H/G, I/H.
The cleaning effect of the magnetic particle chemiluminescent cleaning liquid is calculated by the ratio of B/A, C/B, E/D, F/E, H/G, I/H.
Example 47
This example differs from example 45 in that in this example the amount of surfactant is 4.3%.
Example 48
This example differs from example 45 in that in this example the amount of surfactant is 5.0%.
Example 49
This example differs from example 45 in that in this example the amount of surfactant is 6.5%.
The average value detection results of examples 47 to 49 are shown in Table 8;
table 8 average summary of samples A, B, C, D, E, F, G, H and I in examples 47-49
The average ratio detection results of examples 47 to 49 are shown in Table 9;
table 9 summary of the ratio of the means of samples A, B, C, D, E, F, G, H and I in examples 47-49
Analysis of results:
from the above average ratio, the average ratio of example 45 was higher than that of the other examples, and thus it was found that the magnetic particle chemiluminescent washing liquid provided in example 45 of the present application had the best washing effect.
Magnetic particle chemiluminescence cleaning solution stability test
Any 5 kinds of magnetic particle chemiluminescent cleaning solutions are selected from the magnetic particle chemiluminescent cleaning solutions provided in examples 1-49 for the stability test.
The 5 kinds of magnetic particle chemiluminescent cleaning solutions are the magnetic particle chemiluminescent cleaning solutions provided in examples 1, 15, 30, 45 and 49, respectively.
In this experiment, the magnetic particle chemiluminescent cleaning solutions provided in example 1, example 15, example 30, example 45 and example 49 were respectively packaged in multiple bottles and randomly stored in an environment of 0-50 °, the color of the cleaning solution was observed every day, and PCT positive property control products with the same concentration were tested 10 times to examine the storage stability of the cleaning solutions of the respective examples. The test results are shown in table 10 below;
TABLE 10 summary of PCT cationic control concentration and CV values
From the above table 10, it can be seen that the magnetic particle chemiluminescent cleaning solution disclosed herein is less affected by the environment and temperature, and can be stored in the environment of 0-50 ° for a long time for reuse, without affecting the effect, and at the same time, the CV% after cleaning is less than 10%, which meets the requirements of clinical examination.
The above embodiments are not intended to limit the scope of the present invention, so: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.
Claims (10)
1. The magnetic particle chemiluminescent cleaning solution is characterized by comprising a surfactant, a buffer solution, a preservative, chloride salt, a defoaming agent and water;
wherein the mass fraction of the surfactant is 4.3% -6.8%;
the buffer solution comprises Tris buffer solution and sulfamic acid, and the total mass fraction is 0.5-1.5%;
the preservative comprises Proclin-300, and the total mass fraction is 0.05-0.1%;
the chloride salt comprises NaCl, and the total mass fraction is 0.7-1.5%;
the total mass fraction of the defoaming agent is 0.8-1.5%;
the surfactant comprises a cationic surfactant, a nonionic surfactant and an amphoteric surfactant;
the cationic surfactant is one of lauryl sulfosuccinic acid monoester disodium and monolauryl phosphate;
the nonionic surfactant comprises tween-20; the amphoteric surfactant includes an amino acid type surfactant.
2. A magnetic particle chemiluminescent cleaning solution of claim 1 wherein the magnetic particle comprises a fluorescent dye,
the mass fraction of the surfactant is 5.3% -6.5%;
the total mass fraction of the buffer solution is 0.5-1.5%;
the total mass fraction of the preservative is 0.05-0.1%;
the total mass fraction of the chloride salt is 0.7-1.5%;
the total mass fraction of the defoaming agent is 0.8-1.5%.
3. A magnetic particle chemiluminescent cleaning solution of claim 1 wherein the magnetic particle comprises a fluorescent dye,
the mass fraction of the surfactant is 6.2%;
the total mass fraction of the buffer solution is 1.2%;
the total mass fraction of the preservative is 0.1%;
the total mass fraction of the chloride salt is 1.3%;
the total mass fraction of the defoamer is 1.2%.
4. The magnetic particle chemiluminescent cleaning solution of claim 1 wherein the mass ratio of cationic surfactant, nonionic surfactant and amphoteric surfactant is 2:3:3-5.
5. The magnetic particle chemiluminescent cleaning solution of claim 4 wherein the mass ratio of cationic surfactant, nonionic surfactant, and amphoteric surfactant is 2:3:5.
6. The magnetic particle chemiluminescent cleaning solution of claim 1 wherein the mass fraction of Tris buffer to sulfamic acid is 12:7-10.
7. The magnetic particle chemiluminescent cleaning solution of claim 6 wherein the mass fraction ratio of Tris buffer to sulfamic acid is 3:2.
8. A magnetic particle chemiluminescent cleaning solution of claim 1 wherein the defoamer comprises a silicone oil defoamer and a polyether defoamer.
9. The magnetic particle chemiluminescent cleaning solution of claim 8 wherein the mass ratio of silicone oil-type defoamer to polyether-type defoamer is 1:1.
10. A method of preparing a magnetic particle chemiluminescent cleaning solution of claim 1 comprising the steps of:
preparing a buffer solution with the pH value of 7.4+/-0.2, sequentially adding chloride salt, a surfactant, a preservative and a defoaming agent into the buffer solution, uniformly mixing, and adding ultrapure water to a constant volume of 1L to obtain the magnetic particle chemiluminescent cleaning solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310364388.2A CN116103095A (en) | 2023-04-07 | 2023-04-07 | Magnetic particle chemiluminescent cleaning solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310364388.2A CN116103095A (en) | 2023-04-07 | 2023-04-07 | Magnetic particle chemiluminescent cleaning solution and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116103095A true CN116103095A (en) | 2023-05-12 |
Family
ID=86265816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310364388.2A Pending CN116103095A (en) | 2023-04-07 | 2023-04-07 | Magnetic particle chemiluminescent cleaning solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116103095A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060111266A1 (en) * | 2004-11-08 | 2006-05-25 | Conopco Inc, D/B/A Unilever | Liquid detergent composition |
| CN111607465A (en) * | 2020-06-08 | 2020-09-01 | 珠海丽珠试剂股份有限公司 | Chemiluminescent cleaning solution and preparation method and application thereof |
| CN112159733A (en) * | 2020-09-25 | 2021-01-01 | 芯朗道(天津)医疗科技有限责任公司 | Cleaning solution for magnetic particle chemiluminescence immunoassay and preparation method thereof |
| CN113136269A (en) * | 2021-03-26 | 2021-07-20 | 宁波紫园医疗器械有限公司 | Cleaning solution for chemiluminescence immunoassay |
| JP2023029086A (en) * | 2021-08-20 | 2023-03-03 | 恭治 栗木 | Aide for preparing medical machine washing composition by blending with chlorine-based oxidant and medical machine washing composition using the same |
-
2023
- 2023-04-07 CN CN202310364388.2A patent/CN116103095A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060111266A1 (en) * | 2004-11-08 | 2006-05-25 | Conopco Inc, D/B/A Unilever | Liquid detergent composition |
| CN111607465A (en) * | 2020-06-08 | 2020-09-01 | 珠海丽珠试剂股份有限公司 | Chemiluminescent cleaning solution and preparation method and application thereof |
| CN112159733A (en) * | 2020-09-25 | 2021-01-01 | 芯朗道(天津)医疗科技有限责任公司 | Cleaning solution for magnetic particle chemiluminescence immunoassay and preparation method thereof |
| CN113136269A (en) * | 2021-03-26 | 2021-07-20 | 宁波紫园医疗器械有限公司 | Cleaning solution for chemiluminescence immunoassay |
| JP2023029086A (en) * | 2021-08-20 | 2023-03-03 | 恭治 栗木 | Aide for preparing medical machine washing composition by blending with chlorine-based oxidant and medical machine washing composition using the same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110862881B (en) | Special cleaning solution or diluent for full-automatic chemiluminescence determinator and preparation method thereof | |
| CN107449748B (en) | High-density lipoprotein cholesterol detection kit and use method thereof | |
| CA2032053C (en) | Method for quantitation of calcium and magnesium | |
| CN111607465B (en) | Chemiluminescent cleaning solution and preparation method and application thereof | |
| CN108546602B (en) | Cleaning solution for chemiluminescence immunoassay and magnetic particle chemiluminescence immunoassay detection method | |
| CN113136269A (en) | Cleaning solution for chemiluminescence immunoassay | |
| EP2633289B1 (en) | Use of signal enhancing compounds in electrochemiluminescence detection | |
| CN111896731A (en) | Cleaning solution for chemiluminescence immunoassay | |
| CN116286205B (en) | Tripropylamine buffer solution matched with Luo Cobas e801 chemiluminescent immunoassay analyzer and preparation method thereof | |
| EP2554991B1 (en) | Method for reducing interference by component outside latex immunoagglutination assay system | |
| KR20110042182A (en) | Cystatin c adsorption inhibitor | |
| KR20070001937A (en) | Measurement value lowering inhibitor for immunoassay method and immunoassay method using the same | |
| CN108458981A (en) | A kind of method that methyl hydroximic acid photometry detects iron content in water sample | |
| CN112362432B (en) | Dissociation agent universally used in serum TT3 and TT4 and preparation method thereof | |
| CN113671169A (en) | Non-specific adsorption cleaning solution for chemiluminescence immunoassay AEP | |
| CN116103095A (en) | Magnetic particle chemiluminescent cleaning solution and preparation method thereof | |
| CN113030458B (en) | Sample diluent for ELISA detection kit and preparation method thereof | |
| CN110865182B (en) | Blocking agent and application thereof in immunodetection | |
| CN113484306B (en) | Magnetic particle chemiluminescence cleaning solution and preparation method and application thereof | |
| CN115340910A (en) | Cleaning solution suitable for alkaline phosphatase chemiluminescence system | |
| CN112924438A (en) | Chemiluminescent cleaning buffer solution | |
| CN1443309A (en) | Insoluble carrier particle nephelometric immunoassay reagent | |
| CN117720971A (en) | Cleaning solution for chemiluminescent immunoassay and chemiluminescent immunoassay method | |
| CN111239126A (en) | Detection reagent combination for phosphate molybdenum antimony resistance method determination and preparation method thereof | |
| CN117740765A (en) | Cleaning liquid suitable for chemiluminescent system and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20230512 |
|
| RJ01 | Rejection of invention patent application after publication |