CN116102694A - Integral crystal gel medium embedded with bacterial cellulose and preparation method thereof - Google Patents

Integral crystal gel medium embedded with bacterial cellulose and preparation method thereof Download PDF

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Publication number
CN116102694A
CN116102694A CN202310039196.4A CN202310039196A CN116102694A CN 116102694 A CN116102694 A CN 116102694A CN 202310039196 A CN202310039196 A CN 202310039196A CN 116102694 A CN116102694 A CN 116102694A
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bacterial cellulose
solution
crystal gel
crystal
embedded
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李小年
贠军贤
楼小玲
张文静
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Zhejiang University of Technology ZJUT
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Zhejiang University of Technology ZJUT
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F285/00—Macromolecular compounds obtained by polymerising monomers on to preformed graft polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F251/00—Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof
    • C08F251/02—Macromolecular compounds obtained by polymerising monomers on to polysaccharides or derivatives thereof on to cellulose or derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The invention discloses an integral crystal gel medium embedded with bacterial cellulose and a preparation method thereof, wherein the internal pore diameter of the crystal gel medium is 10-300 microns, the porosity is 70-88%, and the permeability is 10 ‑12 ~10 ‑13 m 2 The method comprises the steps of carrying out a first treatment on the surface of the After the crystal glue is chemically modified, bovine serum albumin is used as a model protein, so that the crystal glue has higher adsorption capacity. The crystal gel takes hydroxyethyl methacrylate embedded with bacterial cellulose particles as a framework, and is grafted with an anion functional group. The crystal gel medium provided by the invention has good biocompatibility, wherein bacterial cellulose can be biodegraded, and the crystal gel medium is prepared fromThe bacterial cellulose particles are embedded to obtain more functional groups, the specific surface area is increased, the mechanical strength of the crystal gel medium is further enhanced, the repeated utilization is facilitated, and the method has a good application prospect in the field of biological separation.

Description

Integral crystal gel medium embedded with bacterial cellulose and preparation method thereof
Technical Field
The invention belongs to the field of biochemical separation, and particularly relates to an integral crystal gel medium embedded with bacterial cellulose and a preparation method thereof.
Background
The crystal gel is a porous material prepared by freezing crystallization pore-forming and monomer polymerization or crosslinking reaction, has excellent performances of good elasticity, ultra-large pores, high specific surface area, high permeability and the like, and can be recycled. The crystal glue has large amount of very large pores of tens to hundreds microns, large pore space and small mass transfer resistance. The crystal gum matrix is mostly natural or biocompatible materials, and has wide application prospect in the field of biological separation. The bacterial cellulose has a unique 3D nano structure, large specific surface area, high water holding rate, high chemical purity, high polymerization degree and high tensile strength, contains abundant hydroxyl groups, has good biocompatibility and biodegradability, and can be used as a new biological separation medium and a new carrier for high-density culture of microbial cells.
The conventional crystal gum medium has few adsorption points and low adsorption capacity, and bacterial cellulose is embedded on the crystal gum medium due to the special structural characteristics, so that the specific surface area can be effectively increased, the crystal gum contains more hydroxyl groups, the grafting reaction is facilitated, more functional groups are obtained, and the separation efficiency is improved; meanwhile, the mechanical property of the crystal glue can be increased, and the crystal glue is favorable for repeated use, but no related report is found.
Disclosure of Invention
Aiming at the technical problems existing in the prior art, the invention aims to provide an integral crystal gel medium embedded with bacterial cellulose and a preparation method thereof.
The integral crystal gel medium embedded with bacterial cellulose takes hydroxyethyl methacrylate and bacterial cellulose as a composite framework, the porosity is 70-85%, the aperture is 10-300 microns, and the water phase permeability is 10 -12 ～10 -13 m 2 。
The preparation method of the integral crystal gum medium embedded with bacterial cellulose comprises the following steps:
s1: dispersing hydroxyethyl methacrylate HEMA and polyethylene glycol diacrylate PEG-DA into ultrapure water according to the mass ratio of 70-80:30-20 to prepare a monomer solution, wherein the total mass fraction of HEMA and PEG-DA in the monomer solution is 10-15%;
s2: adding bacterial cellulose particles into the monomer solution in the step S1, wherein the feeding amount of the bacterial cellulose particles is 0.5-25% of the total mass of HEMA and PEG-DA, and stirring and dispersing uniformly;
s3: adding an initiator and an accelerator into the particle solution uniformly stirred in the step S2, continuously stirring uniformly, rapidly filling the reaction solution into the microcothin tube pre-cooled at-5 to-10 ℃, setting the temperature of a cooling system to-20 to-30 ℃, and continuously reacting for 24 to 48 hours at low temperature to obtain a composite crystal gel column; taking out the microcapillary, thawing at room temperature, and washing the composite crystal gel column with a large amount of pure water for standby;
s4: and (3) grafting the monomer with the tertiary amino functional group onto the composite crystal gel column obtained in the step (S3) through a grafting reaction under the action of a catalyst to obtain the anionic embedded bacterial cellulose integral crystal gel medium, namely, the preparation is completed.
Further, in the step S1, the mass ratio of HEMA to PEG-DA is 75-77:25-23.
Further, in step S2, the preparation method of the bacterial cellulose particles includes: soaking the bacterial cellulose membrane in 5-10% sodium hydroxide solution for 2-4 h, washing with pure water, boiling in water bath at 90-100 ℃ for 0.5-1 h, taking out the bacterial cellulose membrane, preparing particles with the aperture smaller than 500 microns, and squeezing out water to obtain the finished product.
Further, in the step S2, the feeding amount of the bacterial cellulose particles is 5-15% of the total mass of HEMA and PEG-DA. The particle size of the bacterial cellulose particles is below 500 microns.
Further, in the step S3, the initiator and the accelerator are ammonium persulfate and tetramethyl ethylenediamine, respectively, and the dosages of the initiator and the accelerator are 0.5-1.5% of the total mass of HEMA and PEG-DA, preferably 1.0%.
Further, in step S4, the monomer having a tertiary amino functional group in the grafting reaction is dimethylaminoethyl methacrylate.
Further, in step S4, the specific process of the grafting reaction is: and (3) loading the catalyst solution to flow through the prepared crystal gum matrix, fully contacting the catalyst solution with the crystal gum matrix, loading the monomer solution with tertiary amino functional groups to flow through the crystal gum matrix, fully contacting the monomer with tertiary amino functional groups with the crystal gum matrix, controlling the temperature to be 45-60 ℃, reacting for 1-3 h, and flushing the unreacted solution with pure water to obtain the anionic embedded bacterial cellulose integral crystal gum medium.
Further, the volume dosage of the monomer solution is 3-5 times of the volume of the composite crystal gel column, the monomer concentration is 1-2M, and the catalyst solution is Cu 3+ The concentration of the ionic solution is 0.03-0.05M.
Compared with the prior art, the invention has the beneficial technical effects that:
1. according to the invention, bacterial cellulose particles are introduced into the composite crystal gel medium, and have a nano-pore structure, so that the specific surface area can be effectively increased by adding the bacterial cellulose particles. Meanwhile, the bacterial cellulose particles are rich in hydroxyl groups, and the hydroxyethyl methacrylate also has hydroxyl groups, so that the reaction of grafted anion functional groups is more facilitated, and meanwhile, the bacterial cellulose can increase the mechanical strength of the crystal glue, and compared with the conventional crystal glue, the crystal glue is less prone to collapse under high flow rate or after repeated use. In a word, the composite crystal gel medium provided by the invention contains more functional groups, which is beneficial to improving the separation efficiency.
2. The crystal gel medium provided by the invention has better biocompatibility, wherein bacterial cellulose embedded in the crystal gel medium can be biodegraded, and meanwhile, the mechanical strength of the crystal gel medium is further enhanced, so that the crystal gel medium is favorable for recycling.
Detailed Description
The invention will be further illustrated with reference to specific examples, but the scope of the invention is not limited thereto.
When the composite crystal gel medium is filled in the separation column for sample loading, if the mechanical strength of the composite crystal gel medium is high, the collapse of the composite crystal gel medium in the column can not be caused even if the flow rate of the sample loading liquid is high, so that the highest sample loading flow rate which can keep the column not collapsing when the sample loading liquid is stable is provided in the embodiment of the invention. The mechanical performance of the composite crystal glue medium can be described by comparing the highest flow velocity.
In the following examples, the initiator and accelerator were ammonium persulfate and tetramethyl ethylenediamine, respectively.
Example 1
Soaking bacterial cellulose membrane in 5% sodium hydroxide solution for 4 hr, washing with pure water, decocting in water bath at 90deg.C for 1 hr, taking out bacterial cellulose membrane, making into granule with pore diameter less than 500 μm, and squeezing to remove water. Adding pure water into hydroxyethyl methacrylate and polyethylene glycol diacrylate according to the mass ratio of 77:23 to prepare a monomer solution, wherein the total mass concentration of the mixed solution of the hydroxyethyl methacrylate and the polyethylene glycol diacrylate is 15% (w/w). The extruded water bacterial cellulose particles are added into the monomer solution which is uniformly stirred according to the proportion, and the bacterial cellulose particles account for 0.5 percent of the total mass of the (HEMA and PEG-DA) monomers. Adding an initiator and an accelerator into the uniformly stirred particle solution, wherein the initiator and the accelerator account for 1.0% (w/w) of the total amount of monomers (HEMA and PEG-DA), continuously stirring for 1min, rapidly filling the reaction solution into the microcothin tubes which are pre-cooled at the temperature of-5 ℃, setting the temperature of a cooling system to be-20 ℃, and continuously reacting for 48h at low temperature; taking out the micropipe, thawing at room temperature, and washing the composite crystal gel column with a large amount of pure water. The composite crystal gel column is subjected to grafting reaction, a catalyst solution is firstly loaded and flows through a prepared crystal gel matrix, then a monomer solution is loaded and flows through the crystal gel matrix, a grafting monomer is dimethylaminoethyl methacrylate, the concentration of the monomer aqueous solution is 1M, the dosage of the monomer aqueous solution is 3 times of the volume of the composite crystal gel column, and the grafting reaction catalyst is 0.03MCu 3+ The dosage of the ionic solution and the catalyst is 3 times of the volume of the composite crystal gel column, the grafting reaction temperature is 45 ℃, and the reaction time is 3 hours. The obtained composite crystal gel medium has the porosity of 85%, the pore diameter of 10-300 microns and the water phase permeability of 7.1 multiplied by 10 -13 m 2 . Adding deionized water into bovine serum albumin to prepare a BSA solution with the concentration of 6mg/mL, and detecting to obtain the compound crystal gum medium with the adsorption capacity of 3.4mg/mL (wet crystal gum) on the bovine serum albumin as a sample loading liquid.
The composite gel column of example 1 was loaded with a prepared solution of 6mg/ml BSA, and the maximum flow rate at which the gel was able to maintain the structure undeformed and collapsed was up to 12cm/min.
Example 2
Soaking bacterial cellulose membrane in 10% sodium hydroxide solution for 2 hr, washing with pure water, decocting in 100deg.C water bath for 0.5 hr, taking out bacterial cellulose membrane, making into granule with pore diameter less than 500 μm, and squeezing to remove water. Adding pure water into hydroxyethyl methacrylate and polyethylene glycol diacrylate according to the proportion of 77:23 to prepare a monomer solution, wherein the total mass concentration of the mixed solution of the hydroxyethyl methacrylate and the polyethylene glycol diacrylate is 10% (w/w). The extruded water bacterial cellulose particles are added into the monomer solution which is uniformly stirred according to the proportion, wherein the bacterial cellulose particles account for 10 percent of the total mass of the (HEMA and PEG-DA) monomers. Adding an initiator and an accelerator into the uniformly stirred particle solution, wherein the initiator and the accelerator account for 1.0% (w/w) of the total amount of monomers (HEMA and PEG-DA), continuously stirring for 2min, rapidly filling the reaction solution into the microcothin tubes which are pre-cooled at-10 ℃, setting the temperature of a cooling system to-30 ℃, and continuously reacting for 24h at low temperature; taking out the micropipe, thawing at room temperature, and washing the composite crystal gel column with a large amount of pure water. The composite crystal gel column is subjected to grafting reaction, a catalyst solution is firstly loaded and flows through a prepared crystal gel matrix, then a monomer solution is loaded and flows through the crystal gel matrix, a grafting monomer is dimethylaminoethyl methacrylate, the concentration of the monomer aqueous solution is 1M, the dosage of the monomer aqueous solution is 5 times of the volume of the composite crystal gel column, and the grafting reaction catalyst is 0.05MCu 3+ The dosage of the ionic solution and the catalyst is 5 times of the volume of the composite crystal gel column, the grafting reaction temperature is 60 ℃, and the reaction time is 2 hours. The obtained composite crystal gel medium has the porosity of 88 percent, the aperture of 10 to 300 microns and the water phase permeability of 5.6X10 -12 m 2 . Adding deionized water into bovine serum albumin to prepare BSA solution with the concentration of 1mg/mL, and detecting to obtain the compound crystal gum medium with the adsorption capacity of 7.4mg/mL (wet crystal gum) on the bovine serum albumin as a sample loading liquid.
The composite gel column of example 2 was loaded with 1mg/ml BSA solution, and the highest flow rate at which the gel was able to maintain the structure undeformed and collapsed was 13cm/min.
Example 3
Soaking bacterial cellulose membrane in 10% sodium hydroxide solution for 2 hrWashing with pure water, decocting in 100deg.C water bath for 1 hr, taking out bacterial cellulose membrane, making into granule with pore size less than 500 μm, and squeezing to remove water. Adding pure water into hydroxyethyl methacrylate and polyethylene glycol diacrylate according to the proportion of 77:23 to prepare a monomer solution, wherein the total mass concentration of the mixed solution of the hydroxyethyl methacrylate and the polyethylene glycol diacrylate is 15% (w/w). The extruded water bacterial cellulose particles are added into the monomer solution which is uniformly stirred according to the proportion, wherein the bacterial cellulose particles account for 6 percent of the total mass of the (HEMA and PEG-DA) monomers. Adding an initiator and an accelerator into the uniformly stirred particle solution, wherein the initiator and the accelerator account for 1.0% (w/w) of the total amount of monomers (HEMA and PEG-DA), continuously stirring for 1min, rapidly filling the reaction solution into the microcothin tubes which are pre-cooled at the temperature of-5 ℃, setting the temperature of a cooling system to be-20 ℃, and continuously reacting for 48h at low temperature; taking out the micropipe, thawing at room temperature, and washing the composite crystal gel column with a large amount of pure water. The composite crystal gel column is subjected to grafting reaction, a catalyst solution is firstly loaded and flows through a prepared crystal gel matrix, then a monomer solution is loaded and flows through the crystal gel matrix, a grafting monomer is dimethylaminoethyl methacrylate, the concentration of the monomer aqueous solution is 2M, the dosage of the monomer aqueous solution is 3 times of the volume of the composite crystal gel column, and the grafting reaction catalyst is 0.03MCu 3+ The dosage of the ionic solution and the catalyst is 5 times of the volume of the composite crystal gel column, the grafting reaction temperature is 45 ℃, and the reaction time is 2 hours. The obtained composite crystal gel medium has 75 percent of porosity, 10 to 300 microns of pore diameter and 2.3 multiplied by 10 of water phase permeability -13 m 2 . Adding deionized water into bovine serum albumin to prepare a BSA solution with the concentration of 8mg/mL, and detecting to obtain the compound crystal gum medium with the adsorption capacity of 5.4mg/mL (wet crystal gum) on the bovine serum albumin as a sample loading liquid.
The compound gel column of example 3 was loaded with 8mg/ml of the prepared BSA solution, and the maximum flow rate at which the gel can keep the structure undeformed and collapsed was up to 18cm/min.
Example 4
Soaking bacterial cellulose membrane in 10% sodium hydroxide solution for 2 hr, washing with pure water, decocting in 100deg.C water bath for 2 hr, taking out bacterial cellulose membrane, making into granule with pore diameter less than 500 μm, and squeezingMoisture content. Adding pure water into hydroxyethyl methacrylate and polyethylene glycol diacrylate according to the proportion of 77:23 to prepare a monomer solution, wherein the total mass concentration of the mixed solution of the hydroxyethyl methacrylate and the polyethylene glycol diacrylate is 15% (w/w). The extruded water bacterial cellulose particles are added into the monomer solution which is uniformly stirred according to the proportion, wherein the bacterial cellulose particles account for 25 percent of the total mass of the (HEMA and PEG-DA) monomers. Adding an initiator and an accelerator into the uniformly stirred particle solution, wherein the initiator and the accelerator account for 1.0% (w/w) of the total amount of monomers (HEMA and PEG-DA), continuously stirring for 2min, rapidly filling the reaction solution into the microcothin tubes which are pre-cooled at-10 ℃, setting the temperature of a cooling system to-20 ℃, and continuously reacting for 48h at low temperature; taking out the micropipe, thawing at room temperature, and washing the composite crystal gel column with a large amount of pure water. The composite crystal gel column is subjected to grafting reaction, a catalyst solution is firstly loaded and flows through a prepared crystal gel matrix, then a monomer solution is loaded and flows through the crystal gel matrix, a grafting monomer is dimethylaminoethyl methacrylate, the concentration of the monomer aqueous solution is 2M, the dosage of the monomer aqueous solution is 3 times of the volume of the composite crystal gel column, and the grafting reaction catalyst is 0.05MCu 3+ The dosage of the ionic solution and the catalyst is 3 times of the volume of the composite crystal gel column, the grafting reaction temperature is 45 ℃, and the reaction time is 2 hours. The obtained composite crystal gel medium has the porosity of 70 percent, the aperture of 10 to 300 microns and the water phase permeability of 2.4 multiplied by 10 -13 m 2 . Adding deionized water into bovine serum albumin to prepare a BSA solution with the concentration of 6mg/mL, and detecting to obtain the compound crystal gum medium with the adsorption capacity of 2.9mg/mL (wet crystal gum) on the bovine serum albumin as a sample loading liquid.
The composite gel column of example 4 was loaded with a prepared 6mg/ml BSA solution, and the highest flow rate at which the gel was able to maintain the structure undeformed and collapsed was up to 13cm/min.
Example 5
Adding pure water into hydroxyethyl methacrylate and polyethylene glycol diacrylate according to the proportion of 77:23 to prepare a monomer solution, wherein the total mass concentration of the mixed solution of the hydroxyethyl methacrylate and the polyethylene glycol diacrylate is 15% (w/w). Adding an initiator and an accelerator into the uniformly stirred solution, wherein the initiator is initiatedThe agent and the accelerator account for 1.0% (w/w) of the total amount of the monomers (HEMA and PEG-DA), stirring is continued for 2min, the reaction liquid is rapidly poured into the microcothin tube which is pre-cooled at the temperature of minus 10 ℃, the temperature of a cooling system is set to minus 20 ℃, and the reaction is carried out continuously at low temperature for 48h; taking out the micropipe, thawing at room temperature, and washing the gel column with a large amount of pure water. The preparation method comprises the steps of carrying out grafting reaction on a crystal gel column, firstly, loading a catalyst solution to flow through a prepared crystal gel matrix, then loading a monomer solution to flow through the crystal gel matrix, wherein a grafting monomer is dimethylaminoethyl methacrylate, the concentration of a monomer aqueous solution is 1M, the dosage of the monomer aqueous solution is 3 times of the volume of the composite crystal gel column, and the grafting reaction catalyst is 0.05MCu 3+ The dosage of the ionic solution and the catalyst is 3 times of the volume of the composite crystal gel column, the grafting reaction temperature is 45 ℃, and the reaction time is 2 hours. The obtained crystal gel medium has a porosity of 87%, a pore diameter of 10-300 micrometers, and a water phase permeability of 5.8X10 -13 m 2 The maximum flow rate can be up to 3cm/min. Adding deionized water into bovine serum albumin to prepare BSA solution with the concentration of 1mg/mL, and detecting to obtain the composite crystal gel medium with the adsorption capacity of 1.7mg/mL (wet crystal gel) on the bovine serum albumin as a sample loading liquid.
The composite gel column of example 5 was loaded with 1mg/ml BSA solution, and the highest flow rate at which the gel was able to maintain the structure undeformed and collapsed was only 3cm/min.
What has been described in this specification is merely an enumeration of possible forms of implementation for the inventive concept and may not be considered limiting of the scope of the present invention to the specific forms set forth in the examples.

Claims (9)

1. The integral crystal gel medium embedded with the bacterial cellulose is characterized in that the crystal gel medium takes hydroxyethyl methacrylate and the bacterial cellulose as a composite framework, the porosity is 70-85%, the aperture is 10-300 microns, and the water phase permeability is 10 -12 ~10 -13 m 2 。
2. The method for preparing the integral crystal gum medium embedded with bacterial cellulose as claimed in claim 1, which is characterized by comprising the following steps:
s1: dispersing hydroxyethyl methacrylate HEMA and polyethylene glycol diacrylate PEG-DA into ultrapure water according to the mass ratio of 70-80:30-20 to prepare a monomer solution, wherein the total mass fraction of HEMA and PEG-DA in the monomer solution is 10-15%;
s2: adding bacterial cellulose particles into the monomer solution in the step S1, wherein the feeding amount of the bacterial cellulose particles is 0.5-25% of the total mass of HEMA and PEG-DA, and stirring and dispersing uniformly;
s3: adding an initiator and an accelerator into the particle solution uniformly stirred in the step S2, continuously stirring uniformly, rapidly filling the reaction solution into the microcothin tubes pre-cooled at-5 to-10 ℃, setting the temperature of a cooling system to-20 to-30 ℃, and continuously reacting at low temperature to 48 to obtain a composite crystal gel matrix; taking out the microcapillary, thawing at room temperature, and washing the composite crystal gel matrix with a large amount of pure water for standby;
s4: and (3) grafting the monomer with the tertiary amino functional group onto the composite crystal gel matrix obtained in the step (S3) through a grafting reaction under the action of a catalyst to obtain the anionic embedded bacterial cellulose integral crystal gel medium, namely, the preparation is completed.
3. The method for preparing the integral crystal gum medium embedded with bacterial cellulose according to claim 2, wherein in the step S1, the mass ratio of HEMA to PEG-DA is 75-77:25-23.
4. The preparation method of the integral crystal gum medium embedded with bacterial cellulose as claimed in claim 2, wherein in the step S2, the feeding amount of bacterial cellulose particles is 5-15% of the total mass of HEMA and PEG-DA; the particle size of the bacterial cellulose particles is below 500 microns.
5. The method for preparing the integral crystal gum medium embedded with bacterial cellulose as claimed in claim 2, wherein in the step S3, the initiator and the accelerator are ammonium persulfate and tetramethyl ethylenediamine respectively, and the dosage of the initiator and the accelerator is 0.5-1.5% of the total mass of HEMA and PEG-DA, preferably 1.0%.
6. The method for preparing a bacterial cellulose-embedded monolithic gel medium according to claim 2, wherein in the step S4, the monomer with a tertiary amino functional group in the grafting reaction is dimethylaminoethyl methacrylate.
7. The method for preparing the integral crystal gum medium embedded with bacterial cellulose according to claim 2, wherein the preparation method of the bacterial cellulose particles is as follows: colt-shaped bacillus hanshiKomagataeibacter hansenii Soaking the bacterial cellulose membrane obtained by Y21DM fermentation in 5-10% sodium hydroxide solution for 2-4 h, washing with pure water, boiling in water bath at 90-100 ℃ for 0.5-1 h, taking out the bacterial cellulose membrane, preparing particles with the pore diameter smaller than 500 microns, and squeezing out water to obtain the bacterial cellulose membrane.
8. The method for preparing the integral crystal gum medium embedded with bacterial cellulose as claimed in claim 2, wherein in the step S4, the specific process of the grafting reaction is as follows: and loading the catalyst solution to flow through the prepared crystal gel matrix, fully contacting the catalyst solution with the crystal gel matrix, loading the monomer solution with tertiary amino functional groups to flow through the crystal gel matrix, fully contacting the monomer with tertiary amino functional groups with the crystal gel matrix, reacting for a certain time at a certain temperature, and washing the unreacted solution with pure water to obtain the anionic embedded bacterial cellulose integral crystal gel medium.
9. The method of claim 8, wherein in step S4, the monomer solution is 3-5 times the volume of the composite gel column, the monomer concentration is 1-2M, and the catalyst solution is Cu 3+ The concentration of the ionic solution in the grafting solution is 0.03-0.05M, the reaction temperature is 45-60 ℃, and the reaction time is 1-3 h.
CN202310039196.4A 2023-01-13 2023-01-13 Integral crystal gel medium embedded with bacterial cellulose and preparation method thereof Pending CN116102694A (en)

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Application Number Priority Date Filing Date Title
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