CN116083237A - Culture device for colorectal cancer cell culture - Google Patents
Culture device for colorectal cancer cell culture Download PDFInfo
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- CN116083237A CN116083237A CN202310249141.6A CN202310249141A CN116083237A CN 116083237 A CN116083237 A CN 116083237A CN 202310249141 A CN202310249141 A CN 202310249141A CN 116083237 A CN116083237 A CN 116083237A
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- 206010009944 Colon cancer Diseases 0.000 title claims abstract description 22
- 208000001333 Colorectal Neoplasms Diseases 0.000 title claims abstract description 22
- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 230000008859 change Effects 0.000 claims description 37
- 238000009434 installation Methods 0.000 claims description 26
- 230000007246 mechanism Effects 0.000 claims description 12
- 230000006978 adaptation Effects 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 4
- 230000007306 turnover Effects 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 abstract description 7
- 201000011510 cancer Diseases 0.000 abstract description 7
- 230000003068 static effect Effects 0.000 abstract description 3
- 239000013049 sediment Substances 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 8
- 238000003756 stirring Methods 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a culture device for colorectal cancer cell culture, and relates to the technical field of cell culture; the invention improves the problems that sediment can occur in a culture solution caused by static culture, and the hand shake and hand pollution easily occur when the culture solution is replaced, and the invention comprises an incubator and a first placing plate arranged on the inner surface of the incubator, wherein a first culture dish is arranged at the top of the first placing plate, and a replacement pipe penetrates through the bottom of the first culture dish and is connected with the bottom of the first culture dish in a sliding manner; according to the invention, the replacement pipe is downwards moved through the arrangement of the semipermeable membrane, so that the replacement hole enters the culture solution, the culture solution enters the replacement pipe through the replacement hole and falls into the second culture dish through the replacement pipe, so that new culture solution can be added into the first culture dish, the operation is simple and convenient, the toppling is not needed, meanwhile, the problems of hand shake and hand pollution are prevented, cancer cells can be prevented from flowing out along with the culture solution, and the accuracy of experimental data is increased.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a culture device for colorectal cancer cell culture.
Background
In the medical field, it is often necessary to culture cells or bacteria in order to perform medical experiments and help humans to overcome many difficult and complicated diseases, wherein colorectal cancer cell culture is one example, and a related culture device is required to culture the colorectal cancer cells.
When the primary colorectal cancer cells are cultured, the treated culture dishes are placed into a culture box and cultured at a certain temperature and humidity, but the existing culture device for colorectal cancer cell culture has single function, most of the culture devices can only conduct static culture on the culture dishes, so that precipitation can occur in culture solution, cell attachment phenomenon can occur, the culture effect is affected, in addition, the existing culture device for colorectal cancer cell culture is characterized in that cancer cell samples are placed into the culture solution, propagation and maintenance are carried out, in the culture process, workers need to replace the culture solution, most of human operation is carried out on the culture dishes in the existing operation method, so that the culture solution is replaced, but the problems of shaking of hands and hand pollution are easy to occur during replacement, the problem of inconvenience caused by infection of carefully-taken cancer cells and pouring of the culture solution is caused, and the cancer cells are easy to flow out along with the culture solution, so that the number of the cancer cells and the accuracy of experimental data are reduced.
In view of the above problems, the inventors have proposed a culture device for colorectal cancer cell culture for solving the above problems.
Disclosure of Invention
In order to solve the problems that sediment can appear in the culture solution caused by static culture, and shake and hand pollution easily occur when the culture solution is replaced; the invention aims to provide a culture device for colorectal cancer cell culture.
In order to solve the technical problems, the invention adopts the following technical scheme: including incubator and the first board of placing of setting at incubator internal surface, the first top of placing the board is provided with first culture dish, the bottom of first culture dish runs through and sliding connection has the change pipe, change the top of managing the surface and seted up the change hole, the quantity of change hole is provided with a plurality of, the top fixedly connected with collar of changing the pipe surface, the top of collar just is located the periphery fixedly connected with semi-permeable membrane of change hole, the first mounting groove of placing the top of board with change pipe looks adaptation of placing, the internal surface of incubator just is located the below fixedly connected with second of placing the board and places the board, the top of second is provided with the second culture dish, the first top of placing the board is through bracing piece fixedly connected with mounting housing, mounting housing's internal surface fixedly connected with outer collar, the internal surface of outer collar passes through connecting rod fixedly connected with inner mounting plectane, the bottom of inner mounting plectane is provided with rotating mechanism, mounting housing top's both sides are provided with the semi-permeable membrane, and the convenient to change the inside diameter of culture dish that can be added to the change the membrane of change pipe, can change the experiment liquid through the convenient to change the dish that the inside diameter of hand falls into to the change the culture dish, and can change the hand through the convenient to change the experiment liquid to change the dish and prevent the inside the dish from flowing into the change the culture dish, and the change the hand to change the culture dish to the problem.
Preferably, the top fixedly connected with lift thread bush of installation shell, the internal surface threaded connection of lift thread bush has the lift threaded rod, the one end of lift threaded rod runs through installation shell and interior installation plectane in proper order and extends to the outside of interior installation plectane, the one end rotation of lift threaded rod is connected with the lift dwang, the one end fixedly connected with first magnet of lift dwang, the top fixedly connected with and the second magnet of first magnet looks adaptation of change pipe, the front of incubator is provided with the sealing door, and the lift thread bush is used for installing the lift threaded rod, and the lift threaded rod is used for adjusting the height of change pipe, makes the change hole on the change pipe can soak in the culture solution, and first magnet adsorbs the second magnet to it is fixed with the change pipe, when not needing to change the culture solution, prevents that the culture solution from getting into the change intraduct, guarantees the normal operating of device.
Preferably, the rotating mechanism comprises a rotating motor, the bottom of the inner mounting circular plate is connected with a rotating sleeve in an outer turnover way positioned on a lifting rotating rod, one end of the rotating sleeve is fixedly connected with a rotating plate, the top of the rotating plate is provided with a rotating annular sliding groove, the rotating motor is used for driving the rotating sleeve to rotate, the rotating sleeve is used for driving the rotating plate to rotate, the rotating annular sliding groove is used for mounting a rotating thread sleeve, the inner surface of the rotating annular sliding groove is connected with the rotating thread sleeve through a sliding block in a sliding way, the inner surface of the rotating thread sleeve is connected with a rotating threaded rod in a threaded way, the top of the rotating plate penetrates through and is connected with a rotating guide rod in a sliding way, the rotating threaded sleeve is used for mounting a rotating threaded rod, the rotating threaded rod can be driven to rotate and lift by rotating, and the threaded rod can be controlled to enter the first culture dish, thereby can stir the culture solution, the first culture dish is kept away from to the rotation threaded rod simultaneously, makes things convenient for taking out of first culture dish, rotates the effect that the guide arm increases the stirring, carries out spacingly to the rotation threaded rod simultaneously, prevents to rotate the threaded rod along with rotating the thread bush and rotates, guarantees the normal operating of device, fixedly connected with rotates the connection piece between rotation threaded rod and the rotation guide arm, the one end fixedly connected with of rotation motor output shaft rotates output gear, rotate the sheathed tube surface cover establish and fixedly connected with rotate output gear looks adaptation rotate first gear, rotate the connection piece and be used for connecting rotation threaded rod and rotation guide arm, guarantee the normal operating of device, rotate output gear and be used for driving rotation first gear or rotation second gear rotation, make rotation motor can realize two kinds of functions, reduce the use of rotation motor, reduce the manufacturing cost of device, the utility model provides a rotation output gear looks adaptation's rotation second gear is established and fixedly connected with to the surface cover of rotation thread bush, rotation spout has been seted up at the top of interior installation plectane, the internal surface sliding connection who rotates the spout has the rotation slider, the bottom of rotation slider and the top fixed connection of rotation motor, the rotation spout is used for installing spacing rotation slider, the rotation slider is used for connecting rotation motor and rotation regulation thread bush, make rotation motor remove along with rotation regulation thread bush, thereby rotation regulation threaded rod can adjust rotation motor's position, the function of conveniently switching rotation motor, rotation slider's top fixedly connected with rotation regulation thread bush, the top rotation of interior installation plectane is connected with rotation regulation threaded rod, rotation regulation threaded rod's surface and rotation regulation threaded bush's internal surface threaded connection, rotation regulation threaded rod's one end runs through the installation shell and extends to the outside of installation shell, through the setting of slewing mechanism, start rotation motor drives the rotation guide arm and rotates, thereby stir the internal appearance of cell wall phenomenon of preventing to appear in the culture solution, increase the effect of cultivateing simultaneously.
Preferably, the fixture includes the centre gripping pull rod, the one end of centre gripping pull rod runs through the installation shell and extends to the inside of installation shell, the one end of centre gripping pull rod rotates and is connected with centre gripping gangbar, the centre gripping draw-bar has been seted up at the top of outer collar, and the centre gripping pull rod is used for installing the centre gripping gangbar, and the centre gripping gangbar is used for driving the centre gripping sliding block and removes, and the centre gripping sliding groove is used for installing spacing centre gripping sliding block, the interior surface sliding connection of centre gripping sliding groove has the centre gripping sliding block, the one end of centre gripping gangbar rotates with the top of centre gripping sliding block to be connected, the bottom fixedly connected with centre gripping extension rod of centre gripping sliding block, the surface fixedly connected with centre gripping arc rubber slab of centre gripping extension rod is used for connecting centre gripping gangbar and centre gripping extension rod, and centre gripping arc rubber slab are used for centre gripping first culture dish, guarantee the stability of first culture dish, the surface of centre gripping pull rod runs through and sliding connection has the centre gripping locating lever, the surface cover of centre gripping locating lever is equipped with centre gripping locating spring, the one end fixedly connected with the centre gripping thread piece with lift threaded rod looks adaptation, through setting up the top rotation of centre gripping extension rod, thereby the first culture dish of a clamp culture dish of a fixed stability is guaranteed to be increased by setting up and is pushed by a clamp culture dish.
Compared with the prior art, the invention has the beneficial effects that:
1. through the arrangement of the semipermeable membrane, the replacement pipe moves downwards, so that the replacement hole enters the culture solution, the culture solution enters the replacement pipe through the replacement hole and falls into the second culture dish through the replacement pipe, so that new culture solution can be added into the first culture dish, the operation is simple and convenient, dumping is not needed, the problems of hand shake and hand pollution are prevented, cancer cells can be prevented from flowing out along with the culture solution, and the accuracy of experimental data is improved;
2. through the arrangement of the rotating mechanism, the rotating motor is started to drive the rotating plate to enable the rotating guide rod to rotate, so that the culture solution is stirred, precipitation in the culture solution is prevented, meanwhile, the phenomenon of cell adhesion is prevented, and the culture effect is improved;
3. through fixture's setting, pulling centre gripping pull rod drives centre gripping arc rubber slab extrusion first culture dish to it is fixed with first culture dish, guarantees first culture dish's stability, thereby increases the culture effect of device.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic diagram of the overall structure of the present invention.
FIG. 2 is a schematic view showing the internal structure of the incubator of the present invention.
FIG. 3 is a schematic diagram of the structure of a first dish according to the present invention.
FIG. 4 is a schematic view of the structure of the outer mounting ring of the present invention.
Fig. 5 is a schematic view of the structure of the mounting circular plate in the present invention.
Fig. 6 is a schematic structural view of the rotary motor of the present invention.
Fig. 7 is a schematic structural view of the rotating plate of the present invention.
Fig. 8 is a schematic structural view of the clamping linkage of the present invention.
Fig. 9 is a schematic structural view of the lifting threaded rod of the present invention.
FIG. 10 is a schematic view of the structure of the replacement hole of the present invention.
FIG. 11 is a schematic view of a structure of the present invention for clamping an arc-shaped rubber sheet.
Fig. 12 is a schematic structural view of the clamping lever of the present invention.
Fig. 13 is a schematic view of the structure of the mounting housing of the present invention.
Fig. 14 is a schematic view of the structure of the installation groove of the present invention.
In the figure: 1. an incubator; 2. a first placing plate; 3. a first culture dish; 4. replacing the tube; 5. replacing the hole; 6. a mounting ring; 7. a semipermeable membrane; 8. a mounting groove; 9. a second placing plate; 10. a second culture dish; 11. mounting a shell; 12. an outer mounting ring; 13. a circular plate is arranged in the inner part; 14. lifting the threaded sleeve; 15. lifting the threaded rod; 16. a lifting rotating rod; 17. a first magnet; 18. a second magnet; 19. sealing the door; 120. a rotating mechanism; 121. a rotating motor; 122. rotating the sleeve; 123. a rotating plate; 124. rotating the annular chute; 125. rotating the threaded sleeve; 126. rotating the threaded rod; 127. rotating the guide rod; 128. rotating the connecting sheet; 129. rotating the output gear; 1210. rotating the first gear; 1211. rotating the second gear; 1212. rotating the chute; 1213. rotating the sliding block; 1214. rotating the adjusting thread sleeve; 1215. rotating an adjusting threaded rod; 130. a clamping mechanism; 131. clamping the pull rod; 132. clamping the linkage rod; 133. clamping the sliding groove; 134. clamping the sliding block; 135. clamping the extension rod; 136. clamping the arc-shaped rubber plate; 137. clamping the positioning rod; 138. clamping and positioning springs; 139. clamping and positioning the threaded block.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-14, the invention provides a culture device for colorectal cancer cell culture, which comprises a culture box 1 and a first placing plate 2 arranged on the inner surface of the culture box 1, wherein the top of the first placing plate 2 is provided with a first culture dish 3, the bottom of the first culture dish 3 is penetrated and connected with a replacement tube 4 in a sliding manner, the top of the outer surface of the replacement tube 4 is provided with a replacement hole 5, the number of the replacement holes 5 is provided with a plurality of, the top of the outer surface of the replacement tube 4 is fixedly connected with a mounting ring 6, the top of the mounting ring 6 is fixedly connected with a semipermeable membrane 7 positioned on the periphery of the replacement hole 5, the top of the first placing plate 2 is provided with a mounting groove 8 matched with the replacement tube 4, the inner surface of the culture box 1 is fixedly connected with a second placing plate 9 positioned below the first placing plate 2, the top of the second placing plate 9 is provided with a second culture dish 10, the top of the first placing plate 2 is fixedly connected with a mounting shell 11 through a support rod, the inner surface of the mounting shell 11 is fixedly connected with an outer mounting ring 12, the inner surface of the outer mounting ring 12 is fixedly connected with a connecting rod, the inner surface of the outer mounting ring 12 is fixedly connected with a mounting shell 13, the inner surface of the mounting ring 13 is rotatably provided with a new culture medium is arranged through the second round plate 13, and the second round plate 4 is conveniently placed into the second culture medium 4 through the second mounting plate 4, and the second mounting plate 4 is convenient to be provided with a new culture medium, and the second culture medium is required to be filled into the second culture medium through the second culture medium, and has a new culture medium is provided with a new culture medium, and has a new fluid to be filled into the culture medium, and has a new culture medium has been filled into the fresh medium, and has been filled into the fresh medium has been filled in the culture medium has and has.
The top fixedly connected with lift thread bush 14 of installation shell 11, the internal surface threaded connection of lift thread bush 14 has lift threaded rod 15, the one end of lift threaded rod 15 runs through installation shell 11 and interior installation plectane 13 in proper order and extends to the outside of interior installation plectane 13, the one end rotation of lift threaded rod 15 is connected with lift dwang 16, the one end fixedly connected with first magnet 17 of lift dwang 16, the top fixedly connected with of change pipe 4 and the second magnet 18 of first magnet 17 looks adaptation, the front of incubator 1 is provided with sealing door 19.
Through adopting above-mentioned technical scheme, lift thread bush 14 is used for installing lift threaded rod 15, and lift threaded rod 15 is used for adjusting the height of changing pipe 4, makes change hole 5 on the change pipe 4 can submergence in the culture solution, and first magnet 17 adsorbs second magnet 18 to it is fixed with changing pipe 4, when not needing to change the culture solution, prevent that the culture solution from getting into in the change pipe 4, guarantee the normal operating of device.
The rotating mechanism 120 comprises a rotating motor 121, the bottom of the inner mounting circular plate 13 is positioned at the outer turnover of the lifting rotating rod 16 and is connected with a rotating sleeve 122 in a rotating manner, one end of the rotating sleeve 122 is fixedly connected with a rotating plate 123, and the top of the rotating plate 123 is provided with a rotating annular chute 124.
By adopting the above technical scheme, the rotary motor 121 is used for driving the rotary sleeve 122 to rotate, the rotary sleeve 122 is used for driving the rotary plate 123 to rotate, and the rotary annular chute 124 is used for installing the rotary threaded sleeve 125.
The inner surface of the rotary annular chute 124 is slidably connected with a rotary threaded sleeve 125 through a slider, the inner surface of the rotary threaded sleeve 125 is threadedly connected with a rotary threaded rod 126, and the top of the rotary plate 123 is penetrated and slidably connected with a rotary guide rod 127.
Through adopting above-mentioned technical scheme, rotate thread bush 125 and be used for installing and rotate threaded rod 126, rotate the thread bush 125 and rotate threaded rod 126 lift that can drive to can control to rotate threaded rod 126 and get into in the first culture dish 3, thereby can stir the culture solution, rotate threaded rod 126 simultaneously and keep away from first culture dish 3, make things convenient for taking out of first culture dish 3, rotate the effect that guide arm 127 increased the stirring, it is spacing simultaneously to rotate threaded rod 126, prevent to rotate threaded rod 126 along with rotating threaded bush 125 rotation, guarantee the normal operating of device.
A rotary connecting piece 128 is fixedly connected between the rotary threaded rod 126 and the rotary guide rod 127, one end of the output shaft of the rotary motor 121 is fixedly connected with a rotary output gear 129, and the outer surface of the rotary sleeve 122 is sleeved with and fixedly connected with a rotary first gear 1210 which is matched with the rotary output gear 129.
Through adopting above-mentioned technical scheme, rotate the connection piece 128 and be used for connecting rotation threaded rod 126 and rotation guide arm 127, guarantee the normal operating of device, rotate output gear 129 and be used for driving rotation first gear 1210 or rotate second gear 1211 and rotate, make rotation motor 121 can realize two kinds of functions, reduce rotation motor 121's use, reduction device's manufacturing cost.
The outer surface of the rotary thread sleeve 125 is sleeved with and fixedly connected with a rotary second gear 1211 matched with the rotary output gear 129, the top of the inner mounting circular plate 13 is provided with a rotary sliding chute 1212, the inner surface of the rotary sliding chute 1212 is slidably connected with a rotary sliding block 1213, and the bottom of the rotary sliding block 1213 is fixedly connected with the top of the rotary motor 121.
Through the adoption of the technical scheme, the rotating chute 1212 is used for installing the limiting rotating slide block 1213, the rotating slide block 1213 is used for connecting the rotating motor 121 and the rotating adjusting threaded sleeve 1214, so that the rotating motor 121 moves along with the rotating adjusting threaded sleeve 1214, and the rotating adjusting threaded rod 1215 can adjust the position of the rotating motor 121, so that the function of the rotating motor 121 can be conveniently switched.
The top of the rotating slider 1213 is fixedly connected with a rotating adjusting screw sleeve 1214, the top of the inner mounting circular plate 13 is rotatably connected with a rotating adjusting threaded rod 1215, the outer surface of the rotating adjusting threaded rod 1215 is in threaded connection with the inner surface of the rotating adjusting screw sleeve 1214, and one end of the rotating adjusting threaded rod 1215 penetrates through the mounting housing 11 and extends to the outside of the mounting housing 11.
Through adopting above-mentioned technical scheme, through the setting of slewing mechanism 120, start rotation motor 121 drives rotation board 123 and makes rotation guide arm 127 rotate to stir the culture solution, prevent to appear in the culture solution and deposit, prevent simultaneously that the phenomenon of cell adherence from appearing, increase the effect of cultivateing.
The clamping mechanism 130 comprises a clamping pull rod 131, one end of the clamping pull rod 131 penetrates through the installation shell 11 and extends to the inside of the installation shell 11, one end of the clamping pull rod 131 is rotatably connected with a clamping linkage rod 132, and a clamping sliding groove 133 is formed in the top of the outer installation ring 12.
Through adopting above-mentioned technical scheme, clamping pull rod 131 is used for installing clamping linkage rod 132, and clamping linkage rod 132 is used for driving clamping slider 134 to remove, and clamping sliding groove 133 is used for installing spacing clamping slider 134.
The inner surface sliding connection of centre gripping sliding tray 133 has centre gripping sliding block 134, and the one end and the top rotation of centre gripping sliding block 134 of centre gripping gangbar 132 are connected, and the bottom fixedly connected with centre gripping extension rod 135 of centre gripping sliding block 134, the surface fixedly connected with centre gripping arc rubber slab 136 of centre gripping extension rod 135.
Through adopting above-mentioned technical scheme, the centre gripping sliding block 134 is used for connecting centre gripping gangbar 132 and centre gripping extension rod 135, and centre gripping arc rubber slab 136 is used for centre gripping first culture dish 3, guarantees the stability of first culture dish 3.
The outer surface of the clamping pull rod 131 penetrates through and is connected with a clamping and positioning rod 137 in a sliding manner, a clamping and positioning spring 138 is sleeved on the outer surface of the clamping and positioning rod 137, and one end of the clamping and positioning rod 137 is fixedly connected with a clamping and positioning threaded block 139 matched with the lifting threaded rod 15.
Through adopting above-mentioned technical scheme, through fixture 130's setting, pulling centre gripping pull rod 131 drives centre gripping arc rubber slab 136 extrusion first culture dish 3 to it is fixed with first culture dish 3, guarantees the stability of first culture dish 3, thereby increases the culture effect of device.
Working principle: placing the first culture dish 3 on the top of the first placing plate 2, driving the replacement tube 4 to enter the first placing plate 2 through the mounting groove 8 by the first culture dish 3, and pulling the replacement tube 4 to move upwards after the placement is finished, wherein the replacement tube 4 drives the second magnet 18 to be adsorbed by the first magnet 17, so that the height of the replacement tube 4 is limited;
pulling the clamping and positioning rod 137 to enable the clamping and positioning spring 138 to stretch, enabling the clamping and positioning rod 137 to drive the clamping and positioning threaded block 139 to be far away from the lifting threaded rod 15, so that limiting of the clamping and positioning rod 131 is eliminated, pulling the clamping and positioning rod 131 to drive the clamping and linkage rod 132 to rotate, enabling the clamping and linkage rod 132 to drive the clamping and sliding block 134 to move along the clamping and sliding groove 133, enabling the clamping and extending rod 135 to enable the clamping and arc-shaped rubber plate 136 to move, enabling the clamping and arc-shaped rubber plate 136 to be in tight contact with the outer surface of the first culture dish 3, limiting and fixing the first culture dish 3, loosening the clamping and positioning rod 137, enabling the clamping and positioning spring 138 to shrink to drive the clamping and positioning rod 137 to enable the clamping and positioning threaded block 139 to be meshed with the lifting threaded rod 15, limiting of the clamping and positioning rod 131, and preventing the clamping and pulling rod 131 from moving randomly;
starting the rotation motor 121 to drive the rotation output gear 129 to rotate the rotation first gear 1210, driving the rotation sleeve 122 to rotate the rotation plate 123 by the rotation first gear 1210, driving the rotation threaded sleeve 125 to rotate the rotation threaded rod 126 by the rotation plate 123, driving the rotation connecting piece 128 to rotate the rotation guide rod 127 by the rotation threaded rod 126, and slowly stirring the culture solution;
when the culture solution needs to be replaced, the lifting threaded rod 15 is rotated to enable the lifting rotating rod 16 to descend, the lifting rotating rod 16 drives the first magnet 17 to enable the second magnet 18 to descend, the second magnet 18 drives the replacement tube 4 to descend, the replacement tube 4 moves downwards, the replacement hole 5 enters the culture solution, cancer cells are filtered by the semipermeable membrane 7, the culture solution enters the replacement tube 4 through the replacement hole 5, and the culture solution falls into the second culture dish 10 through the replacement tube 4, so that new culture solution can be added into the first culture dish 3;
when the first culture dish 3 needs to be taken out, the moving exchange tube 4 drives the second magnet 18 to be far away from the first magnet 17, the rotating adjusting threaded rod 1215 rotates to enable the rotating adjusting threaded sleeve 1214 to drive the rotating sliding block 1213 to move along the rotating sliding groove 1212, the rotating sliding block 1213 drives the rotating motor 121 to enable the rotating output gear 129 to move, when the rotating output gear 129 is meshed with the rotating second gear 1211, the rotating motor 121 is started to drive the rotating output gear 129 to enable the rotating second gear 1211 to rotate, the rotating threaded rod 126 is driven to rise by the rotating threaded rod 126, the rotating threaded rod 126 drives the rotating guide rod 127 to rise, and accordingly the rotating guide rod 127 is located in a gap between the outer mounting ring 12 and the inner mounting circular plate 13, the rotating guide rod 127 is enabled to move out of the first culture dish 3, the arc-shaped rubber plate 136 is clamped loose in the same way, limiting of the first culture dish 3 is relieved, and the first culture dish 3 is taken out.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (10)
1. The utility model provides a colorectal cancer cell culture is with culture apparatus, includes incubator (1) and sets up at first board (2) of placing of incubator (1) internal surface, its characterized in that: the top of first place board (2) is provided with first culture dish (3), the bottom of first culture dish (3) runs through and sliding connection has change pipe (4), change hole (5) have been seted up at the top of change pipe (4) surface, the quantity of change hole (5) is provided with a plurality of, the top fixedly connected with collar (6) of change pipe (4) surface, the top of collar (6) just is located the periphery fixedly connected with semipermeable membrane (7) of change hole (5), mounting groove (8) with change pipe (4) looks adaptation are seted up at the top of first place board (2), the internal surface of incubator (1) just is located the below fixedly connected with second place board (9) of first place board (2), the top of second place board (9) is provided with second culture dish (10), the top of first place board (2) is through bracing piece fixedly connected with mounting shell (11), the inner surface connection of mounting shell (11) has collar (12) and the inner surface connection of mounting shell (12), the inner disc (13) have the connecting rod (13) through the inner surface connection, clamping mechanisms (130) are arranged on two sides of the top of the installation shell (11).
2. The culture device for colorectal cancer cell culture according to claim 1, characterized in that the top of the installation shell (11) is fixedly connected with a lifting thread sleeve (14), the inner surface of the lifting thread sleeve (14) is in threaded connection with a lifting threaded rod (15), one end of the lifting threaded rod (15) sequentially penetrates through the installation shell (11) and the inner installation circular plate (13) and extends to the outside of the inner installation circular plate (13), one end of the lifting threaded rod (15) is rotationally connected with a lifting rotating rod (16), one end of the lifting rotating rod (16) is fixedly connected with a first magnet (17), the top of the replacement tube (4) is fixedly connected with a second magnet (18) matched with the first magnet (17), and the front surface of the incubator (1) is provided with a sealing door (19).
3. The culture device for colorectal cancer cell culture according to claim 2, wherein the rotating mechanism (120) comprises a rotating motor (121), a rotating sleeve (122) is connected to the bottom of the inner mounting circular plate (13) and is positioned on the outer turnover of the lifting rotating rod (16), one end of the rotating sleeve (122) is fixedly connected with a rotating plate (123), and a rotating annular chute (124) is formed in the top of the rotating plate (123).
4. A culture apparatus for colorectal cancer cell culture according to claim 3, wherein the inner surface of the rotary annular chute (124) is slidably connected with a rotary screw sleeve (125) through a slide block, the inner surface of the rotary screw sleeve (125) is screwed with a rotary threaded rod (126), and the top of the rotary plate (123) is penetrated and slidably connected with a rotary guide rod (127).
5. The culture device for colorectal cancer cell culture according to claim 4, wherein a rotation connection piece (128) is fixedly connected between the rotation threaded rod (126) and the rotation guide rod (127), one end of an output shaft of the rotation motor (121) is fixedly connected with a rotation output gear (129), and an outer surface of the rotation sleeve (122) is sleeved and fixedly connected with a rotation first gear (1210) matched with the rotation output gear (129).
6. The culture device for colorectal cancer cell culture according to claim 5, wherein the outer surface of the rotating threaded sleeve (125) is sleeved and fixedly connected with a rotating second gear (1211) which is matched with the rotating output gear (129), the top of the inner mounting circular plate (13) is provided with a rotating sliding groove (1212), the inner surface of the rotating sliding groove (1212) is slidably connected with a rotating sliding block (1213), and the bottom of the rotating sliding block (1213) is fixedly connected with the top of the rotating motor (121).
7. The culture device for colorectal cancer cell culture according to claim 6, wherein a rotation adjusting screw sleeve (1214) is fixedly connected to the top of the rotation slider (1213), a rotation adjusting threaded rod (1215) is rotatably connected to the top of the inner mounting circular plate (13), the outer surface of the rotation adjusting threaded rod (1215) is in threaded connection with the inner surface of the rotation adjusting screw sleeve (1214), and one end of the rotation adjusting threaded rod (1215) penetrates through the mounting housing (11) and extends to the outside of the mounting housing (11).
8. The culture device for colorectal cancer cell culture according to claim 1, wherein the clamping mechanism (130) comprises a clamping pull rod (131), one end of the clamping pull rod (131) penetrates through the installation shell (11) and extends into the installation shell (11), one end of the clamping pull rod (131) is rotatably connected with a clamping linkage rod (132), and a clamping sliding groove (133) is formed in the top of the outer installation ring (12).
9. The culture device for colorectal cancer cell culture according to claim 8, wherein the inner surface of the clamping sliding groove (133) is slidably connected with a clamping sliding block (134), one end of the clamping linkage rod (132) is rotatably connected with the top of the clamping sliding block (134), the bottom of the clamping sliding block (134) is fixedly connected with a clamping extension rod (135), and the outer surface of the clamping extension rod (135) is fixedly connected with a clamping arc-shaped rubber plate (136).
10. The culture device for colorectal cancer cell culture according to claim 9, wherein the outer surface of the clamping pull rod (131) penetrates through and is connected with a clamping and positioning rod (137) in a sliding manner, the outer surface of the clamping and positioning rod (137) is sleeved with a clamping and positioning spring (138), and one end of the clamping and positioning rod (137) is fixedly connected with a clamping and positioning threaded block (139) matched with the lifting threaded rod (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310249141.6A CN116083237A (en) | 2023-03-15 | 2023-03-15 | Culture device for colorectal cancer cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310249141.6A CN116083237A (en) | 2023-03-15 | 2023-03-15 | Culture device for colorectal cancer cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116083237A true CN116083237A (en) | 2023-05-09 |
Family
ID=86202750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310249141.6A Withdrawn CN116083237A (en) | 2023-03-15 | 2023-03-15 | Culture device for colorectal cancer cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116083237A (en) |
-
2023
- 2023-03-15 CN CN202310249141.6A patent/CN116083237A/en not_active Withdrawn
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