CN116083207A

CN116083207A - Microorganism enriched liquid flow control system and method

Info

Publication number: CN116083207A
Application number: CN202310083162.5A
Authority: CN
Inventors: 严心涛; 王策; 何帅; 裴智果; 宋飞飞; 马玉婷; 陈忠祥; 钟金凤; 王耀
Original assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Current assignee: Suzhou Institute of Biomedical Engineering and Technology of CAS
Priority date: 2023-02-08
Filing date: 2023-02-08
Publication date: 2023-05-09

Abstract

The invention discloses a microorganism enrichment liquid flow control system, which belongs to the field of water area microorganism analysis, wherein an exhaust branch can exhaust air in a first liquid storage bag, a sampling branch can extract a sample to the first liquid storage bag, a dyeing branch can extract a dyeing liquid to dye the sample in the first liquid storage bag, a liquid supply branch is connected with an inlet of a microfluidic chip and pumps the dyed sample in the first liquid storage bag to the microfluidic chip, a waste liquid exhaust branch is communicated with a waste liquid port of the microfluidic chip, and waste liquid of the microfluidic chip is exhausted; the collector is communicated with a sorting outlet of the microfluidic chip and collects microorganisms sorted by the microfluidic chip, and the cleaning liquid component, the fifth pump and the collector form a cleaning branch to wash the microorganisms collected on the collector; the cleaning solution assembly, the fifth pump and the collector can also form a transfer branch for transferring microorganisms from the collector to the storage outlet, and the application also relates to a microorganism-enriched liquid flow control method implemented by the control system.

Description

Microorganism enriched liquid flow control system and method

Technical Field

The invention relates to the field of water area microorganism analysis, in particular to a microorganism enrichment liquid flow control system and a method.

Background

A number of documents indicate that one of the most common research methods for studying microbial ecology in lakes or oceans is based on sampling, analyzing the microbial population at the time of sampling, or bringing the sample back to the laboratory for enrichment culture and analysis by simulation techniques. For example, patent CN202022765498 or CN202021014863 discloses a microorganism collector in water, i.e. directly collecting and storing a water sample in water. The water sample is generally directly subjected to anaerobic refrigeration after being collected, but the in-situ pressure cannot be ensured in the high-pressure culture pre-process from early in-situ fidelity sampling to sample preservation and transfer, laboratory sample segmentation and the like. Particularly, the period from sampling to culturing of the scientific research ship is long, and many barophilic microorganisms die during the period and cannot be separated, so that the analysis of an in-situ ecological system is seriously influenced.

The microfluidic chip technology can integrate basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes onto a micron-scale chip, and can complete the whole analysis process. The acoustic tweezers technology based on the principle that particles move towards nodes in a standing wave sound field has the function of fixing the positions of the particles, and can realize the functions of focusing and sorting the particles in fluid. For example, the micro-fluidic chips in the published patent CN201911348149 and CN201910493156 have the functions of analyzing and enriching microorganisms, but an external liquid flow system matched with the micro-fluidic chip generally adopts manual control or semi-automatic control, so that long-time in-situ analysis and enrichment of microorganisms are difficult to complete in lakes or oceans.

Disclosure of Invention

In order to overcome the defects of the prior art, one of the purposes of the invention is to provide an automatic liquid flow control system based on a microfluidic chip, which comprises full-flow control of bubble removal, quantitative sampling, biological reagent dyeing, analysis and sorting, sample cleaning, sample storage and the like of a water sample of a lake or ocean.

In order to overcome the defects of the prior art, the second aim of the invention is to provide an automatic liquid flow control method based on a microfluidic chip, which comprises the whole flow control of bubble removal, quantitative sampling, biological reagent dyeing, analysis and sorting, sample cleaning, sample storage and the like of a water sample of a lake or ocean.

One of the purposes of the invention is realized by adopting the following technical scheme:

the front-end liquid flow structure comprises a first liquid storage bag, a sampling branch, a dyeing branch, a liquid supply branch and an exhaust branch, wherein the sampling branch, the dyeing branch, the liquid supply branch and the exhaust branch are respectively communicated with the first liquid storage bag, the exhaust branch can exhaust air in the first liquid storage bag, the sampling branch can extract samples to the first liquid storage bag, the dyeing branch can extract dyeing liquid to dye the samples in the first liquid storage bag, the liquid supply branch is connected with an inlet of the microfluidic chip and pumps the dyed samples in the first liquid storage bag to the microfluidic chip, and the rear-end liquid flow structure further comprises a waste liquid exhaust branch, a collector, a fifth pump and a cleaning liquid component, wherein the waste liquid exhaust branch is communicated with a waste liquid outlet of the microfluidic chip and is used for exhausting waste liquid of the microfluidic chip; the collector is communicated with the sorting outlet of the microfluidic chip and collects microorganisms sorted by the microfluidic chip, and the cleaning liquid component, the fifth pump and the collector form a cleaning branch to wash the microorganisms collected on the collector; the cleaning liquid assembly, the fifth pump and the collector can also form a transfer branch, the flow direction of the transfer branch in the collector is opposite to the flow direction of the cleaning branch in the collector, and the transfer branch transfers microorganisms from the collector to a storage end outlet.

Further, the sampling branch comprises a pre-filter, a first pump and a first two-way valve which are communicated through a pipeline in sequence, the front end of the pre-filter is a sample inlet, and the tail end of the first two-way valve is communicated with the first liquid storage bag.

Further, the sampling branch further comprises a multi-stage filter, which is located between the pre-filter and the first pump.

Further, the dyeing branch comprises a second two-way valve, a second pump and a second liquid storage bag which are sequentially communicated through a pipeline, wherein the second liquid storage bag stores the dyeing agent, and the second two-way valve is communicated with the first liquid storage bag.

Further, the liquid supply branch comprises a first steering valve and a third pump, the first steering valve is respectively communicated with the first liquid storage bag, the third pump and the microfluidic chip, the third pump is an injection pump, and the third pump can extract samples in the first liquid storage bag and send the samples to the microfluidic chip.

Further, the waste liquid discharge branch comprises a first one-way valve and a three-way joint communicated with the first one-way valve, and the first one-way valve is communicated with a waste liquid port of the microfluidic chip.

Further, the back-end liquid flow structure further comprises a second steering valve, a third steering valve, a fourth steering valve, a fifth steering valve and a third one-way valve, wherein the second steering valve is respectively communicated with the sorting outlet, the collector inlet and the fourth steering valve of the microfluidic chip, the third steering valve is communicated with the collector outlet, the three-way joint, the fifth steering valve and the cleaning liquid component, the fourth steering valve is communicated with the second steering valve, the third one-way valve and the fifth pump, and the fifth steering valve is communicated with the collector inlet, the fifth pump and the cleaning liquid component.

Further, the microorganism enrichment liquid flow control system further comprises a shell, wherein the shell is of a cage chamber structure, the shell comprises a top cover and a plurality of connectors arranged on the top cover, and the connectors are respectively communicated with a sample inlet of the sampling branch, an exhaust end of the exhaust branch, a waste liquid end outlet of the waste liquid exhaust branch and a storage end outlet of the transfer branch.

The second purpose of the invention is realized by adopting the following technical scheme:

a method of controlling a microorganism-rich liquid stream using the microorganism-rich liquid stream control system described above, comprising the steps of:

and (3) discharging bubbles: removing air from the microorganism-enriched liquid stream control system;

quantitative sampling: the sampling branch is opened, and a quantitative sample is extracted from the research water domain to the first liquid storage bag;

staining with biological reagent: the dyeing branch is opened, a dyeing reagent is extracted from the second liquid storage bag to the first liquid storage bag, and the microorganisms in the sample are subjected to dyeing incubation;

analysis and sorting: the liquid supply branch is opened, the dyed and incubated samples in the first liquid storage bag are conveyed to the micro-fluidic chip, the micro-fluidic chip sorts the samples, the sorted samples are conveyed to the collector, and the waste liquid is discharged through the waste liquid discharge branch;

sample cleaning: the cleaning branch is opened, a fifth pump pumps cleaning liquid to enter the collector from the inlet of the collector, and samples on the collector are cleaned;

sample storage: the transfer branch is opened, the fifth pump pumps the cleaning liquid from the collector outlet to the collector, and the fifth pump exits from the collector outlet to transfer the microorganism sample on the collector to the storage end outlet.

Further, the exhaust bubble includes the steps of:

the sampling branch is used for discharging bubbles;

the dyeing branch is used for discharging bubbles;

the waste liquid discharge branch is used for discharging bubbles;

cleaning the branch line to remove bubbles;

the transferring branch is used for discharging bubbles;

the first reservoir bag is vented.

Compared with the prior art, the microorganism enrichment liquid flow control system can realize full-flow automatic control such as bubble removal, quantitative sampling, biological reagent dyeing, analysis and separation, sample cleaning, sample storage and the like in the process of separating the water sample of the lake or the ocean by the microfluidic chip, and can complete long-time in-situ analysis and enrichment of microorganisms in the lake or the ocean.

Drawings

FIG. 1 is a schematic diagram of a first embodiment of a microbial enriched liquid stream control system according to the present invention;

FIG. 2 is a schematic illustration of a front-end liquid flow configuration of the microbial enriched liquid flow control system of FIG. 1;

FIG. 3 is a schematic illustration of the back-end liquid flow configuration of the microbial enriched liquid flow control system of FIG. 1;

FIG. 4 is a perspective view of a microbial enriched liquid stream control system of the present invention;

FIG. 5 is a schematic diagram of a second embodiment of a microbial enriched liquid stream control system according to the present invention.

In the figure: 10. a front end flow structure; 11. a first reservoir bag; 12. sampling branch circuits; 120. a pre-filter; 121. a multi-stage filter; 122. a first pump; 123. a first two-way valve; 13. a dyeing branch; 130. a second two-way valve; 131. a second pump; 132. a second reservoir bag; 14. a liquid supply branch; 140. a first steering valve; 141. a third pump; 15. an exhaust branch; 150. a third two-way valve; 151. a fourth pump; 20. a microfluidic chip; 30. a back-end flow structure; 31. a waste liquid discharge branch; 310. a first one-way valve; 311. a three-way joint; 32. a collector; 33. a cleaning liquid assembly; 330. a cleaning liquid storage bag; 331. a second one-way valve; 34. a fifth pump; 35. a third one-way valve; 36. a second steering valve; 37. a third steering valve; 38. a fourth steering valve; 39. a fifth steering valve; 40. a housing; 41. a top cover; 42. and (3) a joint.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1 to 4, a first embodiment of the microorganism enriched liquid flow control system of the present invention comprises a front-end liquid flow structure 10, a microfluidic chip 20, and a back-end liquid flow structure 30.

The front-end flow structure 10 comprises a first reservoir 11, a sampling branch 12, a dyeing branch 13, a liquid supply branch 14 and a gas discharge branch 15.

The sampling branch 12 is used to draw a cleaning liquid (in this embodiment clear water) or a sample of the area of investigation (in this embodiment water in a lake or sea). The sampling branch 12 includes a prefilter 120, a multistage filter 121, a first pump 122, and a first two-way valve 123, which are sequentially communicated through a pipe. The inlet of the pre-filter 120 is the sample inlet. The outlet of the first two-way valve 123 communicates with the first reservoir 11. The prefilter 120 is a stainless steel or nylon or Polytetrafluoroethylene (PTFE) filter screen with a pore size of 0.5-2 mm.

The staining branch 13 is used to add a stain to the first reservoir 11 to stain the sample. The dyeing branch 13 comprises a second two-way valve 130, a second pump 131 and a second liquid storage bag 132 which are sequentially communicated through pipelines. The second reservoir 132 stores a stain which may be used by SYBRGreen I for the determination of heterotrophic bacteria, for example, as described in patent CN201410171582.X. The outlet of the second two-way valve 130 communicates with the first reservoir 11.

The liquid supply branch 14 is used for extracting the sample in the first liquid storage bag 11 and delivering the sample to the microfluidic chip 20. The liquid supply branch 14 comprises a first diverter valve 140 and a third pump 141. The first diverter valve 140 is respectively communicated with the first liquid storage bag 11, the third pump 141 and the microfluidic chip 20, the third pump 141 is an injection pump, and the third pump 141 can pump the sample in the first liquid storage bag 11 and temporarily store the sample, and then send the sample to the microfluidic chip 20.

The exhaust branch 15 is used for exhausting air in the first liquid storage bag 11. The exhaust branch 15 includes a third two-way valve 150 and a fourth pump 151. The third two-way valve 150 is communicated with the fourth pump 151 through a pipeline, the other end of the third two-way valve 150 is communicated with the first liquid storage bag 11, and an exhaust end is arranged at the outlet of the fourth pump 151.

The structure of the microfluidic chip 20 is as shown in published patents CN201911348149 and CN201910493156, and the acoustic tweezer technology based on the principle that particles move toward nodes in a standing wave sound field has the function of fixing the positions of the particles, and can realize the functions of focusing and sorting the particles in the fluid.

The back-end flow structure 30 includes a waste drain branch 31, a collector 32, a cleaning liquid assembly 33, a fifth pump 34, a third one-way valve 35, a second diverter valve 36, a third diverter valve 37, a fourth diverter valve 38, and a fifth diverter valve 39.

The waste discharge branch 31 includes a first check valve 310 and a three-way joint 311 communicating with the first check valve 310, and the first check valve 310 communicates with the waste port of the microfluidic chip 20. The tail end of the three-way joint 311 is a waste liquid end outlet.

The collector 32 is a stainless steel or nylon or Polytetrafluoroethylene (PTFE) filter membrane with a pore size of 0.2-5 μm.

The cleaning solution assembly 33 is used to provide a water source for cleaning microorganisms and a medium for transferring stored microorganisms. The cleaning liquid assembly 33 includes a cleaning liquid storage bag 330 and a second check valve 331 in communication with the cleaning liquid storage bag 330. The cleaning solution storage bag 330 may be used to store a buffer solution, such as Phosphate Buffered Saline (PBS), as a cleaning solution according to practical application requirements. In the sample washing process of the first embodiment, the respective pipes or pipe joints or diverter valves before and after the collector 32 are washed so that the target microorganisms sorted in the collector 32 are suspended in the buffer. The second check valve 331 is also in communication with the third steering valve 37 and the fourth steering valve 38.

The fifth pump 34 is used to provide motive force for cleaning microorganisms and transferring stored microorganisms. Both ends of the fifth pump 34 are connected to a fourth steering valve 38 and a fifth steering valve 39, respectively.

The third check valve 35 is connected to the fourth steering valve 38, and the end of the third check valve 35 is a storage end outlet.

The second diverter valve 36 is respectively communicated with a sorting outlet of the microfluidic chip 20, an inlet of the collector 32 and a fourth diverter valve 38, the third diverter valve 37 is communicated with an outlet of the collector 32, a three-way joint 42311 and a fifth diverter valve 39, and the cleaning liquid assembly 33, the fourth diverter valve 38 is communicated with the second diverter valve 36, the third one-way valve 35 and the fifth pump 34, and the fifth diverter valve 39 is communicated with the inlet of the collector 32, the fifth pump 34 and the cleaning liquid assembly 33.

The pipeline for communicating in the microorganism enrichment liquid flow control system is made of polyether ether ketone (PEEK) or Polytetrafluoroethylene (PTFE). The outer diameter of the pipeline is 1.6-3.2 mm, and the inner diameter is 0.2-1.0 mm.

The first liquid storage bag 11, the second liquid storage bag 132 and the cleaning liquid storage bag 330 are made of polyvinyl chloride (PVC) or polypropylene (PP) with good stretching performance and biocompatibility.

The first pump 122, the second pump 131, the third pump 141, the fourth pump 151, and the fifth pump 34 are constant flow pumps such as syringe pumps, peristaltic pumps, and diaphragm pumps.

The front-end liquid flow structure 10, the microfluidic chip 20 and the back-end liquid flow structure 30 are mounted inside a housing 40, the housing 40 is a cage structure, the housing 40 includes a top cover 41 and a plurality of connectors 42 disposed on the top cover 41, and the connectors 42 are respectively communicated with a sample inlet of the sampling branch 12, an exhaust end of the exhaust branch 15, a waste liquid end outlet of the waste liquid discharge branch 31 and a storage end outlet of the transfer branch.

In using the microbial enriched liquid stream control system of the present invention, first venting is performed.

The exhaust step specifically comprises the following steps:

(1) The first pump 122, the first two-way valve 123 are open, the remaining valves and pumps are closed, and the operation is performed for a period of time according to specific needs; a volume of water sample is drawn from the sample inlet, the air in each pump or valve or conduit on the left side of the first reservoir 11 (sampling branch 12) is removed, and provision is made for filling the pump or valve or conduit afterwards for removing air therefrom.

(2) The first pump 122, the first two-way valve 123, the second two-way valve 130 and the second pump 131 are opened, the rest of the valves and the pumps are closed, water sample is pumped into the first liquid storage bag 11 from the sample inlet and the second liquid storage bag 132 respectively, and air in each pump or valve or pipeline on the lower side (the dyeing branch 13) of the first liquid storage bag 11 is discharged.

(3) The third pump 141 starts sampling and the other two-way valve or the steering valve or the pump is closed; the third pump 141 draws a certain amount of water from the first reservoir 11 in preparation for the subsequent evacuation of air.

(4) The first steering valve 140 and the third pump 141 are started for proofing, and the other two-way valves or steering valves or pumps are closed; the third pump 141 is used to make the water sample flow through the microfluidic chip 20, the first check valve 310, the three-way joint 311, the second diverter valve 36, the collector 32, the third diverter valve 37 and the air in the pipeline between these devices, and is discharged in the outlet of the waste liquid end.

(5) The second steering valve 36 and the fifth pump 34 are open, and the other two-way valve or steering valve or pump is closed; the water sample is drawn from the cleaning solution reservoir 330, air in the purge line is vented, and vented in the waste side outlet.

(6) The fifth pump 34, the second steering valve 36, the third steering valve 37, the fourth steering valve 38, the fifth steering valve 39 are open, and the other two-way valves or steering valves or pumps are closed; the water sample is drawn from the cleaning fluid reservoir 330, and air from other devices and tubing in the back-end flow structure 30 (transfer branch) is removed and vented to the reservoir outlet.

(7) The third two-way valve 150 and the fourth pump 151 are open, and the other two-way valve or the steering valve or the pump are closed; mainly for exhausting air from the first reservoir 11.

(8) All two-way valves or steering valves or pumps are closed.

In the step of exhausting, whether the bubble exhausting is finished is judged, and whether a sample flows out of the exhaust port is mainly observed, and meanwhile, no bubble flows out any more as a judging standard. For automatic judgment, a bubble detector can be connected to the rear end of the exhaust port, and if no bubbles exist for a certain time, the completion of the bubble exhaust flow can be judged.

And (3) quantitatively sampling:

the first pump 122 and the first two-way valve 123 are opened, the other valves and pumps are closed, and samples are quantitatively extracted according to practical application requirements, and water samples to be detected are extracted from lakes or oceans into the first liquid storage bag 11.

A biological reagent staining step:

(1) The second two-way valve 130 and the second pump 131 are opened, the other valves and pumps are closed, and the dye is quantitatively extracted according to the actual application requirements; the pre-stored dye is drawn from the second reservoir 132 into the first reservoir 11.

(2) All the two-way valves or the steering valves or the pumps are closed, and the microorganisms in the samples are stained and incubated according to the actual application requirements.

Analyzing and sorting:

(1) The third pump 141 starts sampling and the other two-way valve or the steering valve or the pump is closed; the third pump 141 draws a certain amount of sample from the first reservoir 11;

(2) The first steering valve 140 and the third pump 141 are started for proofing, and the other two-way valves or steering valves or pumps are closed; the sample pumped by the third pump 141 is pumped into the microfluidic chip 20 for microbial analysis or sorting, and the detected waste liquid is discharged from the waste liquid outlet.

(3) And (3) circulating the processes (1) and (2) according to the actual application requirements.

Sample cleaning:

the fifth pump 34 and the second steering valve 36 are on and the other two-way valve or steering valve or pump is off. The pre-stored specific buffer solution is extracted from the cleaning solution storage bag 330, the sorted sample in the collector 32 is washed (mainly for marine samples), the sorted specific microorganism is trapped at the front end of the filter screen in the collector 32, and the waste solution is discharged from the waste solution end outlet.

Sample storage:

(1) The fifth pump 34, the second steering valve 36, the third steering valve 37, the fourth steering valve 38, the fifth steering valve 39 are open, and the other two-way valves or steering valves or pumps are closed; the pre-stored specific buffer solution is extracted from the cleaning solution storage bag 330, and microorganisms at the front end of the filter screen in the collector 32 are transferred to the storage-end outlet.

(2) All two-way valves or steering valves or pumps are closed.

With continued reference to fig. 5, in this embodiment, the structure of the microbial enriched liquid stream control system according to the present invention is the same as that of the first embodiment, except that: in this embodiment, the cleaning solution assembly 33 does not include a cleaning solution reservoir 330, and the second one-way valve 331 communicates with the outlet of the multi-stage filter 121, enabling the sampling branch 12 to provide buffer solution to the wash branch as well as the transfer branch.

With continued reference to fig. 5, the present invention also relates to a method for controlling a microorganism-rich liquid stream using the microorganism-rich liquid stream control system, comprising the steps of:

quantitative sampling: the sampling branch 12 is opened, and a quantitative sample is extracted from the research water area to the first liquid storage bag 11;

staining with biological reagent: the staining branch 13 is opened, and a staining reagent is extracted from the second liquid storage bag 132 to the first liquid storage bag 11 to perform staining incubation on microorganisms in the sample;

analysis and sorting: the liquid supply branch 14 is opened, the dyed and incubated sample in the first liquid storage bag 11 is conveyed to the micro-fluidic chip 20, the micro-fluidic chip 20 sorts the sample, the sorted sample is conveyed to the collector 32, and the waste liquid is discharged through the waste liquid discharge branch 31;

sample cleaning: the cleaning branch is opened, the fifth pump 34 pumps cleaning liquid from the inlet of the collector 32 to enter the collector 32, and samples on the collector 32 are cleaned;

sample storage: the transfer branch is opened and the fifth pump 34 pumps the cleaning fluid from the outlet of the collector 32 into the collector 32 and out of the collector 32 from the outlet of the collector 32 to transfer the microbial sample on the collector 32 to the outlet of the storage end.

Further, the exhaust bubble includes the steps of:

the sampling branch 12 is used for discharging bubbles;

the dyeing branch 13 discharges bubbles;

the waste liquid discharge branch 31 discharges bubbles;

cleaning the branch line to remove bubbles;

the transferring branch is used for discharging bubbles;

the first reservoir 11 is vented.

The invention can overcome the problem that the prior art cannot perform in-situ microorganism analysis and enrichment, and provides an automatic liquid flow control system based on a microfluidic chip, which comprises full-flow control of bubble removal, quantitative sampling, biological reagent dyeing, analysis and separation, sample cleaning, sample storage and the like of a water sample of a lake or ocean. The liquid flow control system can realize in-situ microorganism analysis and enrichment of the existing microfluidic chip in a lake or ocean environment. In addition, the liquid flow system of the invention can be free from carrying additional sheath liquid or buffer liquid, thereby being beneficial to long-time running work of the system under water.

The foregoing examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present invention, which are equivalent to the above embodiments according to the essential technology of the present invention, and these are all included in the protection scope of the present invention.

Claims

1. The utility model provides a microorganism enrichment liquid flow control system, includes micro-fluidic chip, its characterized in that: the device comprises a microfluidic chip, a front-end liquid flow structure, a rear-end liquid flow structure, a waste liquid discharge branch, a collector, a fifth pump and a cleaning liquid component, wherein the front-end liquid flow structure comprises a first liquid storage bag, a sampling branch, a dyeing branch, a liquid supply branch and an exhaust branch, the sampling branch, the dyeing branch, the liquid supply branch and the exhaust branch are respectively communicated with the first liquid storage bag, the exhaust branch can discharge air in the first liquid storage bag, the sampling branch can extract samples to the first liquid storage bag, the dyeing branch can extract dyeing liquid to dye the samples in the first liquid storage bag, the liquid supply branch is connected with an inlet of the microfluidic chip and pumps the dyed samples in the first liquid storage bag to the microfluidic chip, and the rear-end liquid flow structure further comprises a waste liquid discharge branch, the collector, the fifth pump and the cleaning liquid component, wherein the waste liquid discharge branch is communicated with a waste liquid outlet of the microfluidic chip and discharges waste liquid of the microfluidic chip; the collector is communicated with the sorting outlet of the microfluidic chip and collects microorganisms sorted by the microfluidic chip, and the cleaning liquid component, the fifth pump and the collector form a cleaning branch to wash the microorganisms collected on the collector; the cleaning liquid assembly, the fifth pump and the collector can also form a transfer branch, the flow direction of the transfer branch in the collector is opposite to the flow direction of the cleaning branch in the collector, and the transfer branch transfers microorganisms from the collector to a storage end outlet.

2. The microbial enriched liquid stream control system of claim 1, wherein: the sampling branch comprises a prefilter, a first pump and a first two-way valve which are communicated through a pipeline in sequence, the front end of the prefilter is a sample inlet, and the tail end of the first two-way valve is communicated with the first liquid storage bag.

3. The microbial enriched liquid stream control system of claim 2, wherein: the sampling branch further includes a multi-stage filter positioned between the pre-filter and the first pump.

4. The microbial enriched liquid stream control system of claim 1, wherein: the dyeing branch comprises a second two-way valve, a second pump and a second liquid storage bag which are sequentially communicated through a pipeline, wherein the second liquid storage bag stores a dyeing agent, and the second two-way valve is communicated with the first liquid storage bag.

5. The microbial enriched liquid stream control system of claim 1, wherein: the liquid supply branch comprises a first steering valve and a third pump, the first steering valve is respectively communicated with the first liquid storage bag, the third pump and the microfluidic chip, the third pump is an injection pump, and the third pump can pump samples in the first liquid storage bag and send the samples to the microfluidic chip.

6. The microbial enriched liquid stream control system of claim 1, wherein: the waste liquid discharge branch comprises a first one-way valve and a three-way joint communicated with the first one-way valve, and the first one-way valve is communicated with a waste liquid port of the microfluidic chip.

7. The microbial enriched liquid stream control system of claim 6, wherein: the back-end liquid flow structure further comprises a second steering valve, a third steering valve, a fourth steering valve, a fifth steering valve and a third one-way valve, wherein the second steering valve is respectively communicated with a sorting outlet, a collector inlet and the fourth steering valve of the microfluidic chip, the third steering valve is communicated with the collector outlet, the three-way joint and the fifth steering valve and the cleaning liquid component, the fourth steering valve is communicated with the second steering valve, the third one-way valve and the fifth pump, and the fifth steering valve is communicated with the collector inlet, the fifth pump and the cleaning liquid component.

8. The microbial enriched liquid stream control system of claim 1, wherein: the microorganism enrichment liquid flow control system further comprises a shell, wherein the shell is of a cage chamber structure, the shell comprises a top cover and a plurality of connectors arranged on the top cover, and the connectors are respectively communicated with a sample inlet of the sampling branch, an exhaust end of the exhaust branch, a waste liquid end outlet of the waste liquid exhaust branch and a storage end outlet of the transfer branch.

9. A method of controlling a microbial enriched liquid stream using the microbial enriched liquid stream control system of any of claims 1-8, comprising the steps of:

10. The method of controlling a microbial enriched liquid stream according to claim 9, wherein: the exhaust bubble comprises the following steps:

the sampling branch is used for discharging bubbles;

the dyeing branch is used for discharging bubbles;

the waste liquid discharge branch is used for discharging bubbles;

cleaning the branch line to remove bubbles;

the transferring branch is used for discharging bubbles;

the first reservoir bag is vented.