CN116077563A - Traditional Chinese medicine composition for treating liver and kidney deficiency type depression and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating liver and kidney deficiency type depression and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating liver and kidney deficiency type depression, which is prepared from the following components in parts by weight: 2-5 parts of uncaria, and 3-6 parts of clerodendranthus spicatus; extracting the white uncaria powder with 40% -75% ethanol to obtain white uncaria concentrated solution and dregs, combining the white uncaria dregs with the clerodendranthus spicatus powder, extracting with acid water with the pH value of 5-6, regulating the pH value of the extracting solution to 7.8-8.5, standing, filtering, collecting precipitate, combining with the white uncaria concentrated solution, and drying to obtain powder; the traditional Chinese medicine composition disclosed by the invention has the effects of soothing liver, regulating qi, nourishing heart, strengthening spleen, warming yang, tonifying kidney, regulating balance of qi, blood, yin and yang of viscera, and treating mild and moderate depression, especially depression caused by deficiency of liver and kidney; can obviously enhance the sugar preference value of mice with chronic stress depression models, increase the number of autonomous activities of forced swimming, reduce the swimming immobility time and shorten the ingestion hiding period.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for treating liver and kidney deficiency type depression and a preparation method thereof.
Background
Depression refers to a disorder of human brain structure and function, which causes different degree of disorder of mental activities such as cognition, emotion, will, etc. under the action of biological, psychological and social factors. Currently, the number of people suffering from depression worldwide has an increasing trend year by year without falling back, and depression is the most popular affective disease in the world today. According to the latest research and study, the prevalence rate of people suffering from depression in China is 3.7% -4.6%, and the situation of rising year by year appears, and in recent years, depression has become a disease which brings serious burden and injury to society and families, and the method for seeking effective and safe treatment of depression has become a difficulty and problem which need to be solved urgently. Therefore, the research of the medicine for enhancing the treatment of depression has important social and economic significance.
Chinese patent document CN103316019B discloses a pharmaceutical combination comprising a selective 5-hydroxytryptamine reuptake inhibitor and a glucocorticoid receptor antagonist for the treatment of depression, in particular a combination comprising an amount of SSRI or a pharmaceutically acceptable salt or solvate thereof and an amount of GR antagonist or a pharmaceutically acceptable salt or solvate thereof, optionally in admixture with one or more pharmaceutically acceptable carriers, for use as a therapy for depression and related disorders.
However, most of the antidepressants synthesized by western medicine have the defects of narrow antidepressant spectrum, large toxic and side effects, high drug price, easy recurrence and the like, so that the research and development of the antidepressants at home and abroad are more and more focused on traditional medicines, in particular to Chinese herbal medicines with the history of more than two thousands of years.
Although there is no clear description of the disease name of "depression" in medicine, depression belongs to common diseases in ancient times, and the syndrome differentiation belongs to "depression", "lily disease", "dysphoria" and the like, and all the diseases belong to the category of depression. The first symptoms of depression are recorded in the book of interior meridian and Ling Shu Su Wen. The Ming Dynasty of medical science adopts the disease name of "Yu Zheng". Traditional Chinese medicine considers that the cause of the depression is due to internal injury of emotion, exogenous six-barrenness and malnutrition; the disease is characterized by excess, the excess is mainly qi stagnation, blood fatigue, fire heat, phlegm stagnation and food stagnation, wherein the qi stagnation is the root cause; the deficiency is mainly due to spleen qi deficiency and heart qi and heart blood deficiency, and the symptoms are complex and changeable, which are caused by the stimulation of emotion.
In the "Chinese medicine disease diagnosis and treatment Standard" issued by the State administration of Chinese traditional medicine, the "melancholy disease" is classified into five types, namely yin deficiency and fire hyperactivity, liver qi stagnation. Modern traditional Chinese medicine considers that the pathogenesis key of depression is depression of qi movement, liver is the main disease location, most of clinically developed pathogenesis is deficiency of heart, spleen and liver and kidney on the basis of liver depression, and the pathogenesis key is based on the thought of soothing liver, regulating qi, nourishing heart, strengthening spleen, warming yang and tonifying kidney, and regulates the balance of qi, blood, yin and yang of viscera. Part of scholars propose the importance of kidney yang in the pathogenesis of depression, so the inventor starts with the aspects of nourishing yin, tonifying kidney and soothing liver and regulating qi by combining with the related theory of traditional Chinese medicine, researches national medicines, and obtains a composition which can effectively relieve liver qi stagnation and nourish yin and tonify kidney through compatibility analysis and research, and has the effect of treating mild and moderate depression, especially depression caused by deficiency of liver and kidney.
Disclosure of Invention
In order to solve the defects existing in the prior art, the invention aims to provide the traditional Chinese medicine composition for treating liver and kidney deficiency type depression and the preparation method thereof, which can sooth liver and regulate qi, nourish heart and strengthen spleen, warm yang and tonify kidney, and condition balance of qi, blood and yin and yang of viscera, has the effect of treating mild and moderate depression, has low cost and small side effect, and is suitable for long-term administration.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a traditional Chinese medicine composition for treating liver and kidney deficiency type depression is prepared from the following components in parts by weight:
2-5 parts of uncaria;
3-6 parts of clerodendranthus spicatus.
Preferably, the traditional Chinese medicine composition for treating liver and kidney deficiency type depression is prepared from the following components in parts by weight: 3 parts of uncaria and 4 parts of clerodendranthus spicatus.
Preferably, the extracts of the raw materials and pharmaceutically acceptable carriers are prepared into granules, powder, tablets, capsules, pills, oral liquid or mixture.
The invention also claims a preparation method of the traditional Chinese medicine composition for treating liver and kidney deficiency type depression, which comprises the following steps:
(1) Crushing: weighing the uncaria alba and the clerodendranthus spicatus according to the weight parts, respectively crushing, and sieving with a 30-60-mesh sieve to obtain uncaria alba powder and clerodendranthus spicatus powder;
(2) Alcohol extraction: extracting the white uncaria powder obtained in the step (1) with ethanol for 2 times, combining the 2 times of extracting solutions, concentrating until no alcohol smell exists, and obtaining white uncaria concentrated solution and dregs for later use;
(3) Acid extraction: mixing the white uncaria dreg obtained in the step (2) with the clerodendranthus spicatus powder obtained in the step (1) to obtain mixed powder, extracting for 2 times by using acid water, mixing the 2 times of extracting solutions, adding alkali to adjust the pH value, standing, filtering, and collecting precipitate;
(4) Combining: and (3) respectively drying the white hooked uncaria concentrated solution obtained in the step (2) and the precipitate obtained in the step (3), grinding into powder, and uniformly mixing to obtain the traditional Chinese medicine composition for treating liver and kidney deficiency type depression.
Preferably, in the step (2), the concentration of the ethanol is 55% -75%.
Preferably, in the step (2), the weight ratio of the white hooked uncaria powder to the ethanol is 1: 10-12, and the single extraction time is 2-4 h.
Preferably, in the step (3), the acid water is hydrochloric acid solution with a pH value of 5-6.
Preferably, in the step (3), the alkali is an ammonia water solution, and the pH of the solution is adjusted to 7.8-8.5 by adding the alkali.
Preferably, in the step (3), the weight ratio of the mixed powder to the acid water is 1: 12-16, and the single extraction time is 1.5-3.5 h.
The invention also claims an application of the traditional Chinese medicine composition in preparing a medicine for treating liver and kidney deficiency type depression.
Compared with the prior art, the invention has the following beneficial effects:
1) The invention discovers that the composition of the uncaria and the clerodendranthus spicatus has the treatment effect on depression for the first time, the composition provided by the invention has the effects of nourishing yin, tonifying kidney and soothing liver and regulating qi, a model of a depressed mouse is duplicated by adopting a chronic mild unpredictable stimulation (CUMS) method, and the activity of each extract and composition prepared by the invention is verified, so that the composition has good anti-depression activity, good anti-depression curative effect, low cost, small side effect, suitability for long-term administration, safety and reliability, and is mainly used for light and medium depression.
2) The uncaria in the traditional Chinese medicine composition provided by the invention has the effects of calming endogenous wind and arresting convulsion, and clearing heat and calming liver; the clerodendranthus spicatus has the effects of clearing heat and eliminating dampness and expelling Dan Lishui, and has good effect on kidney diseases; the traditional Chinese medicine composition provided by the invention has reasonable compatibility, integrates the functions of regulating liver, warming kidney and strengthening spleen, has the effects of soothing the nerves, resolving depression, nourishing heart and relieving restlessness, and can effectively improve sadness emotion of patients with depression.
3) The traditional Chinese medicine composition provided by the invention can increase the expression of the hypothalamic dorsal nucleus beta-endorphin, can obviously enhance the sugar water preference value of a model mouse with chronic stress depression, can increase the number of autonomous activities of forced swimming of the model mouse, reduce the swimming immobility time, shorten the ingestion hiding period, and has obvious effect on treating depression.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following examples. Of course, the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It should be noted that, the preparation of the traditional Chinese medicine composition for treating liver and kidney deficiency type depression in examples 1-3 can be properly selected according to the common auxiliary materials in the pharmaceutical field and according to the dosage form and the actual situation, for example, common auxiliary materials include starch, low-substituted hydroxypropyl cellulose, micro powder silica gel, magnesium stearate, starch slurry, sucrose, dextrin, sodium carboxymethyl starch, talcum powder, polysorbate, polyethylene glycol and the like; in preparing the various dosage forms of the desired drug, the compound may be prepared according to conventional methods of manufacture in the pharmaceutical arts, such as by mixing the compound with one or more carriers and then formulating into the corresponding dosage form; preferably, the dosage forms of the pharmaceutical preparation comprise tablets, pills, tablets, granules, capsules, mixture and the like.
Example 1
A preparation method of a traditional Chinese medicine composition for treating liver and kidney deficiency type depression comprises the following steps:
(1) Crushing: respectively pulverizing 100g of uncaria and 150g of clerodendranthus spicatus, and sieving with a 60-mesh sieve to obtain uncaria powder and clerodendranthus spicatus powder;
(2) Alcohol extraction: extracting ramulus Uncariae cum Uncis powder with 55% ethanol 10 times of weight of ramulus Uncariae cum Uncis powder for 2 times each for 2 hr, mixing extractive solutions, concentrating until no alcohol smell exists, and collecting ramulus Uncariae cum Uncis concentrated solution and residue;
(3) Acid extraction: mixing the white uncaria dregs obtained in the step (2) with the clerodendranthus spicatus powder obtained in the step (1) to obtain mixed powder, extracting the mixed powder for 2 times by using 12 times of hydrochloric acid solution with the pH value of 5.1 and each time for 1.5 hours, mixing 2 times of extracting solutions, adding ammonia water solution to adjust the pH value to 7.8, standing, filtering, and collecting precipitate;
(4) Combining: and (3) respectively drying the white hooked uncaria concentrated solution obtained in the step (2) and the precipitate obtained in the step (3), grinding into powder, and uniformly mixing to obtain the traditional Chinese medicine composition for treating liver and kidney deficiency type depression.
A method for preparing a tablet by using the traditional Chinese medicine combination obtained in example 1, comprising the following steps:
taking 21.33g of the composition obtained in the example 1, grinding into fine powder, adding 21g of 10% starch paste, 7g of medicinal starch and 1g of micro powder silica gel, mixing, sieving with a 14-mesh sieve, granulating, drying at 80 ℃, finishing, adding 0.05g of magnesium stearate, uniformly mixing, and pressing into 100 tablets, wherein the weight of each tablet is 0.5g, and the content of crude drugs in each tablet is 2.5g.
A method for preparing a hard capsule by using the traditional Chinese medicine combination obtained in the embodiment 1, comprising the following steps:
taking 21.51g of the composition obtained in the example 1, grinding into fine powder, adding 29.5g of medicinal starch and 0.01g of magnesium stearate, uniformly mixing, sieving, encapsulating, and making into 100 capsules, wherein the content of the hard capsules is 0.5g, and the content of each capsule contains 2.5g of crude drugs.
A method for preparing a soft capsule by using the traditional Chinese medicine combination obtained in the embodiment 1, comprising the following steps:
20.98g of the composition obtained in example 1 is taken, 0.4g of propylene glycol, 1.6g of lecithin and 28g of soybean oil are added, and the mixture is subjected to vibration grinding for ultra-micro for 20 minutes to uniformly mix, so that 100 capsules are prepared, the content of the soft capsules is 0.5g, and the content of crude drugs in each capsule is 2.5g.
Example 2
A preparation method of a traditional Chinese medicine composition for treating liver and kidney deficiency type depression comprises the following steps:
(1) Crushing: respectively pulverizing 250g of uncaria and 300g of clerodendranthus spicatus, and sieving with a 30-mesh sieve to obtain uncaria powder and clerodendranthus spicatus powder;
(2) Alcohol extraction: extracting ramulus Uncariae cum Uncis powder with 75% ethanol 12 times of weight of ramulus Uncariae cum Uncis powder for 2 times each for 2 hr, mixing extractive solutions, concentrating until no ethanol smell exists, and collecting ramulus Uncariae cum Uncis concentrated solution and residue;
(3) Acid extraction: mixing the white uncaria dregs obtained in the step (2) with the clerodendranthus spicatus powder obtained in the step (1) to obtain mixed powder, extracting the mixed powder for 2 times by using 16 times of hydrochloric acid solution with the pH value of 6 and each time for 1.5 hours, mixing 2 times of extracting solutions, adding ammonia water solution to adjust the pH value to 8.4, standing, filtering, and collecting precipitate;
(4) Combining: and (3) respectively drying the white hooked uncaria concentrated solution obtained in the step (2) and the precipitate obtained in the step (3), grinding into powder, and uniformly mixing to obtain the traditional Chinese medicine composition for treating liver and kidney deficiency type depression.
A method for preparing sustained release tablets by using the traditional Chinese medicine combination obtained in the embodiment 2 comprises the following steps:
taking 31.34g of the composition obtained in the example 2, grinding into fine powder, sieving with a 100-mesh sieve, adding 11.1g of HPMC, 12g of lactose and 1g of sodium dodecyl sulfate, sieving with a 100-mesh sieve, uniformly mixing, adding 2% of povidone adhesive to prepare soft materials, sieving with a 20-mesh sieve, granulating, drying at 50 ℃, sieving with a 18-mesh sieve, granulating, adding 2% of magnesium stearate into the dry granules by an external method, uniformly mixing, tabletting, and preparing 100 tablets, wherein the tablet weight is 0.5g, and the content of crude drugs in each granule is 5.5g.
Example 3
A preparation method of a traditional Chinese medicine composition for treating liver and kidney deficiency type depression comprises the following steps:
(1) Crushing: respectively pulverizing 150g of uncaria alba and 200g of clerodendranthus spicatus, and sieving with a 30-mesh sieve to obtain uncaria alba powder and clerodendranthus spicatus powder;
(2) Alcohol extraction: extracting ramulus Uncariae cum Uncis powder with 75% ethanol 12 times of weight of ramulus Uncariae cum Uncis powder for 2 times each for 2 hr, mixing extractive solutions, concentrating until no ethanol smell exists, and collecting ramulus Uncariae cum Uncis concentrated solution and residue;
(3) Acid extraction: mixing the white uncaria dregs obtained in the step (2) with the clerodendranthus spicatus powder obtained in the step (1) to obtain mixed powder, extracting the mixed powder for 2 times by using 16 times of hydrochloric acid solution with the pH value of 6 and each time for 1.5 hours, mixing 2 times of extracting solutions, adding 0.5mol/L ammonia water solution to adjust the pH value to 8.4, standing, filtering, and collecting precipitate;
(4) Combining: and (3) respectively drying the white hooked uncaria concentrated solution obtained in the step (2) and the precipitate obtained in the step (3), grinding into powder, and uniformly mixing to obtain the traditional Chinese medicine composition for treating liver and kidney deficiency type depression.
A method for preparing granules by using the traditional Chinese medicine combination obtained in the embodiment 3 comprises the following steps:
taking 20.46g of the composition obtained in the example 3, grinding into fine powder, adding 25g of dextrin and 460g of lactose, uniformly mixing, granulating, drying at 80 ℃, finishing, subpackaging, and preparing 100 bags to obtain granules, wherein the content is 5g, and the crude drug content of each granule is 3.5g.
The following are toxicity tests:
selecting 60 healthy mice, wherein the weight of the mice is 28-32 g, randomly dividing the mice into 3 groups, carrying out gastric lavage administration on each group of male and female halves by using the compositions prepared in the examples 1-3, wherein the administration amount is 5mg/g, and each time in the morning and at night; continuously observing 14d, wherein the mice subjected to gastric lavage administration in examples 1-3 have no death or abnormal toxic reaction, have good diet and activity, and have no death; after 90 days of administration, the mice in each group have no abnormal manifestation, active behavior, mao Guanghua backs, normal diet, average weight of 45g, and no significant difference in hemogram, biochemical index, bone marrow and urine conventional index, and organ coefficient.
In addition, the preparation prepared by the prescription of the composition obtained in the example 2 is selected, and through general pharmacological tests such as blood pressure, heart rate, electrocardiogram, respiration and the like of rabbits and central nervous function influence tests respectively under awake and anesthetized states, the blood pressure, heart rate, electrocardiogram, respiration and the pre-administration ratio of animals after administration are found to have no obvious change, and animals are not found to have excitation or inhibition phenomena, so that the administration is safe within the prescription range of the invention.
The following are pharmacological experiments:
chronic unpredictable Wen Heying stimulus (chronic unpredictable mild stress, CUMS) is a rodent stress model commonly used in depression research , and model animal pathophysiological changes caused by modeling are similar to human depression, so that the invention adopts a CUMS method to verify the antidepressant activity of the extract obtained in the example.
The experimental mice were female C57BL/6J mice weighing 30+ -2 g, supplied by Hangzhou son laboratory animal technologies Co., ltd., eligibility number: 20220606Abzz0105000964, adaptive feeding for one week, free feed; feeding conditions: clean-level open animal room, proper temperature and humidity (22-24 ℃, 50-60%), natural light source and 12-hour light and shade alternation (12L: 12D).
1. Grouping animals
SPF-class female C57BL/6J mice were randomly divided into 6 groups (n=10), namely a blank group, a model group, a positive drug (fluoxetine) group, an example 1 group, an example 2 group and an example 3 group, wherein 10 mice in each group are subjected to cage feeding of the blank group and the other groups are subjected to single cage feeding of the other groups.
2. Moulding and administration
After grouping, mice other than the placebo group were modeled, and each group received 21 days of various stimuli, including: inverting 24 hours, fasted 24 hours, water-forbidden 24 hours, inclined 24 hours, wet 24 hours, constraint 2 hours, ice water swimming 3 minutes, hot water swimming 3 minutes, tail clamping 1 minute, electric shock, noise 2 hours, shaking 5 minutes, peculiar smell 24 hours (different peculiar smell every week), foreign matter 24 hours (different foreign matters every week); 2 kinds of stimulation are randomly selected every day, 1-2 times of stimulation are used every week, the same stimulation cannot continuously appear, the stimulation has unpredictability, the gastric lavage administration is started after the stimulation is ended at the 2 nd week, after the administration is ended for 28 days, the emotional behavior detection is carried out after the gastric lavage treatment is ended, and the emotional behavior detection comprises a sugar water preference experiment, a forced swimming experiment and an overhead cross maze experiment. The methods and amounts of administration for each group of mice are shown in Table 1.
Table 1 methods and amounts of administration
3. Evaluation index
(1) General state observation: the mice in the model group gradually appear in a slack and lazy state, loose fur, roughness and no luster, yellow color, arch backs, loose stool, gradually emaciated bodies, positive medicine groups, the mice in the examples 1-3 and blank groups react agilely, white fur and luster, and act sensitively, wherein the mice in the example 2 group die due to stomach filling errors. The specific status of each group of mice is shown in Table 2.
TABLE 2 general State
(2) Sugar water preference experiment: the adaptive training is started on the 25 th day of gastric lavage, the syrup preference test is carried out on the 28 th day, all animals are fed and fasted in a single cage, the time is 1 hour, and the syrup preference ratio of each group of mice is calculated by comparing the consumption of pure water and sucrose water before and after the test:
sugar preference = sugar water consumption/total liquid consumption x 100%
(3) Forced swimming: swimming pre-experiment was performed 24h before the formal experiment, and the mice were put into 4000mL containers with water depth of 25cm and water temperature of 25 ℃ and were swimed for 10min. Carrying out formal experiments on the following day 9:00, putting the mice into the same environment as the pre-swimming, and swimming for 6min; the mice showed passive floating, only the small movements of the limbs remained floating, and struggling behavior disappeared for 3s to be regarded as a stationary state, and the accumulated stationary time of the forced swimming of the mice within 5min after calculation.
(4) Novel inhibition of ingestion: before the start of the experiment, each group of mice was fasted for 24 hours, and the open field experiment box was prepared: a wood box without top is 60cm multiplied by 80cm, the inner four walls of the wood box are coated with black, the box bottom is divided into 8 multiplied by 8 equal parts of small squares by using paint, food is placed at the most center of the box bottom, and the time when the mice are placed in the wood box until the food is found, namely the feeding latency period is measured; note that at the beginning of the experiment, the mouse head was placed towards the wall of the box and the time for the mouse to hold up the food with both forelimbs was observed and timed with a stopwatch.
(5) Detecting index data:
TABLE 3 detection index data
Note that: # p compared to blank group<0.01， ** P compared to model group<0.01。
From the above data, compared with the blank group, the sugar water preference value of the model group before modeling has no significant difference, but the sugar water preference value after treatment is significantly reduced, and the total immobility time and ingestion latency time of forced swimming are significantly increased, thus indicating that modeling is successful; the ratio of sugar water preference values to model groups of the positive drug group and the examples 1-3 is larger, wherein the treatment effect of the example 2 is equivalent to that of the positive drug; the immobility time and the ingestion latency time of forced swimming are obviously shortened compared with those of a model group, wherein the shortening effect of the ingestion latency time is equivalent to that of a positive medicine, the shortening effect of the immobility time of the forced swimming is slightly lower than that of the positive medicine, so that each group of the examples has the treatment effect on depression, the group of the examples 2 has the best effect, and the improvement effect on partial indexes is equivalent to that of the positive medicine.
The traditional Chinese medicine composition disclosed by the invention is applied to treatment of depression, has positive effects on improvement of clinical symptoms and rehabilitation process of diseases, has higher safety and has clinical popularization value.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (10)
1. The traditional Chinese medicine composition for treating liver and kidney deficiency type depression is characterized by comprising the following components in parts by weight:
2-5 parts of uncaria;
3-6 parts of clerodendranthus spicatus.
2. The traditional Chinese medicine composition for treating liver and kidney deficiency type depression according to claim 1 is characterized by being prepared from the following components in parts by weight: 3 parts of uncaria and 4 parts of clerodendranthus spicatus.
3. The traditional Chinese medicine composition for treating liver and kidney deficiency type depression according to claim 1, wherein the extracts of the raw materials and a pharmaceutically acceptable carrier are prepared into granules, powder, tablets, capsules, pills, oral liquid or mixture.
4. A method for preparing a traditional Chinese medicine composition for treating liver and kidney deficiency type depression according to any one of claims 1 to 3, which is characterized by comprising the following steps:
(1) Crushing: weighing the uncaria alba and the clerodendranthus spicatus according to the weight parts, respectively crushing, and sieving with a 30-60-mesh sieve to obtain uncaria alba powder and clerodendranthus spicatus powder;
(2) Alcohol extraction: extracting the white uncaria powder obtained in the step (1) with ethanol for 2 times, combining the 2 times of extracting solutions, concentrating until no alcohol smell exists, and obtaining white uncaria concentrated solution and dregs for later use;
(3) Acid extraction: mixing the white uncaria dreg obtained in the step (2) with the clerodendranthus spicatus powder obtained in the step (1) to obtain mixed powder, extracting for 2 times by using acid water, mixing the 2 times of extracting solutions, adding alkali to adjust the pH value, standing, filtering, and collecting precipitate;
(4) Combining: and (3) respectively drying the white hooked uncaria concentrated solution obtained in the step (2) and the precipitate obtained in the step (3), grinding into powder, and uniformly mixing to obtain the traditional Chinese medicine composition for treating liver and kidney deficiency type depression.
5. The method according to claim 4, wherein in the step (2), the concentration of ethanol is 55% to 75%.
6. The preparation method according to claim 4, wherein in the step (2), the weight ratio of the white uncaria powder to the ethanol is 1: 10-12, and the single extraction time is 2-4 h.
7. The process according to claim 4, wherein in the step (3), the acid water is a hydrochloric acid solution having a pH of 5 to 6.
8. The process according to claim 4, wherein in the step (3), the alkali is an aqueous ammonia solution, and the pH of the solution is adjusted to 7.8 to 8.5 by adding alkali.
9. The method according to claim 4, wherein in the step (3), the weight ratio of the mixed powder to the acid water is 1: 12-16, and the single extraction time is 1.5-3.5 h.
10. Use of a Chinese medicinal composition according to any one of claims 1 to 3 for the preparation of a medicament for the treatment of depression due to deficiency of the liver and kidney.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101890135A (en) * | 2010-07-12 | 2010-11-24 | 浙江工业大学 | Antidepressant traditional Chinese medicine composition and application thereof |
| CN102958529A (en) * | 2009-12-21 | 2013-03-06 | 马来西亚热带生物有限公司 | Orthosiphon stamineus extracts with beneficial use as cognition enhancer |
| CN103446140A (en) * | 2013-09-16 | 2013-12-18 | 中国人民解放军第三军医大学第二附属医院 | Application of rhynchophylline in preparation of drug with anxiolytic effect and antidepressant effect |
| CN104286260A (en) * | 2014-09-29 | 2015-01-21 | 安徽协和成药业饮片有限公司 | Corn stigma tea capable of lowering blood pressure |
| CN104490972A (en) * | 2015-01-06 | 2015-04-08 | 山西大学 | Pharmaceutical composition for treating and preventing depression and preparation method of pharmaceutical composition |
| CN108186637A (en) * | 2018-01-10 | 2018-06-22 | 南方科技大学 | A kind of application of isorhynchophylline in the drug with neuroprotection is prepared |
| CN108245512A (en) * | 2018-01-10 | 2018-07-06 | 南方科技大学 | A kind of application of isocorynoxeine in the drug with neuroprotection is prepared |
| CN114404412A (en) * | 2022-03-03 | 2022-04-29 | 中国人民解放军军事科学院军事医学研究院 | Application of uncaria active compound in preparation of medicine for preventing and/or treating mental disorder diseases |
-
2022
- 2022-11-25 CN CN202211488508.1A patent/CN116077563B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102958529A (en) * | 2009-12-21 | 2013-03-06 | 马来西亚热带生物有限公司 | Orthosiphon stamineus extracts with beneficial use as cognition enhancer |
| CN101890135A (en) * | 2010-07-12 | 2010-11-24 | 浙江工业大学 | Antidepressant traditional Chinese medicine composition and application thereof |
| CN103446140A (en) * | 2013-09-16 | 2013-12-18 | 中国人民解放军第三军医大学第二附属医院 | Application of rhynchophylline in preparation of drug with anxiolytic effect and antidepressant effect |
| CN104286260A (en) * | 2014-09-29 | 2015-01-21 | 安徽协和成药业饮片有限公司 | Corn stigma tea capable of lowering blood pressure |
| CN104490972A (en) * | 2015-01-06 | 2015-04-08 | 山西大学 | Pharmaceutical composition for treating and preventing depression and preparation method of pharmaceutical composition |
| CN108186637A (en) * | 2018-01-10 | 2018-06-22 | 南方科技大学 | A kind of application of isorhynchophylline in the drug with neuroprotection is prepared |
| CN108245512A (en) * | 2018-01-10 | 2018-07-06 | 南方科技大学 | A kind of application of isocorynoxeine in the drug with neuroprotection is prepared |
| CN114404412A (en) * | 2022-03-03 | 2022-04-29 | 中国人民解放军军事科学院军事医学研究院 | Application of uncaria active compound in preparation of medicine for preventing and/or treating mental disorder diseases |
Non-Patent Citations (4)
| Title |
|---|
| 刘松林;徐玉秀;黄燕俊;曾常青;: "钩藤总生物碱对慢性束缚应激小鼠的抗抑郁作用", 广东药科大学学报, vol. 33, no. 01, pages 72 - 76 * |
| 彭朝忠;祁建军;李先恩;: "沧源县佤族医治风湿病验方录", 中国民族民间医药, no. 09, pages 4 * |
| 柳威等: "钩藤提取物及钩藤碱的药理研究进展", 中国新药与临床药理, vol. 32, no. 6, pages 899 - 904 * |
| 王剑等: "肾茶袋泡茶质量标准的提升研究", 中国民族民间医药, vol. 31, no. 10, pages 31 - 36 * |
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