CN116067933B - Biological fluorescence development reagent, and development method and application of biological evidence trace - Google Patents
Biological fluorescence development reagent, and development method and application of biological evidence trace Download PDFInfo
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- CN116067933B CN116067933B CN202310287508.3A CN202310287508A CN116067933B CN 116067933 B CN116067933 B CN 116067933B CN 202310287508 A CN202310287508 A CN 202310287508A CN 116067933 B CN116067933 B CN 116067933B
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Abstract
The invention relates to a biological fluorescence development reagent, a development method and application of a biological evidence trace, which comprises the following steps in percentage by weight: 0.01% -0.7% of indendione and ZnCl 2 0.005-0.02%, 1-6% ethyl acetate, 1-6% methanolThe mixed solution of ethyl acetate and methanol is selected as the dissolution solution of indenedione, and a proper amount of zinc chloride and CFC are added, so that the prepared biological fluorescence development reagent can shorten the required reaction time, and simultaneously, a permeable object or a semi-permeable object can be reacted under the condition of room temperature to develop the biological evidence trace. In addition, the biological fluorescence developing reagent can relieve discomfort caused by pungent smell in the use process of operators, and has higher safety and stability.
Description
Technical Field
The invention relates to a biological fluorescence development reagent, a development method of a biological evidence trace and application thereof.
Background
In the criminal evidence investigation field, criminal evidence left by a suspected person needs to be found and obtained, thereby constructing a criminal fact evidence chain, laying legal evidence foundation for punishing crimes, wherein the criminal evidence comprises fingerprint evidence, palm print evidence and trace evidence capable of reflecting DNA characteristics.
In the prior art, the inventor proposes a method for performing fingerprint development by using indene diketone, the main current bio-development reagent needs to react for 15-30 min, the specific time length needs to be determined according to the site environment, and in addition, the reagent volatilizes rapidly, so that operators feel uncomfortable due to pungent smell. In chinese patent CN104605860B, indene diketone, glacial acetic acid, ethyl acetate and petroleum ether are used as raw materials to prepare a fluorescence development reagent, the permeable object is sprayed, then dried in an environment with the relative humidity of less than 40% and the temperature of 50-120 ℃, the dried permeable object is irradiated by laser with the wavelength of 532nm and the broad-spectrum half-height width of less than 1nm, the illuminance of more than 30 kaleidoscope is controlled to be formed on the surface of the permeable object, and the biological evidence trace on the permeable object can be developed under a light interception filter at 540nm, so that the fingerprints on bricks, sticks, fabrics and leather can be extracted well. However, in the using process, the material evidence treated by the method needs to be heated to a certain temperature and kept stand for more than 15 minutes, and the used fluorescent developing agent has high acidic odor pungency and is easy to generate uncomfortable feeling for operators. In chinese patent CN106802292B, the raw material formulation of the bioluminescence visualizer is: the indene diketone, ethyl acetate, glycerol, pure alcohol and petroleum ether are reacted by indene diketone and sweat ammonia base acid, and the biological fluorescence developing reagent used in the method has no obvious acidic smell and does not generate sour discomfort to operators. However, in the use process of operators, the influence of the ambient temperature and the ambient humidity on the reagent development effect is large, the reagent development effect still needs to be dried for 10-15 min under a certain temperature, and if the operating ambient temperature is low and the humidity is large, the longer the reaction time required for reagent development is, the reaction time cannot be effectively shortened and the pungent smell of the bioluminescence development reagent cannot be reduced while the fluorescence effect is enhanced.
Disclosure of Invention
Based on the problems existing in the prior art, the invention aims to provide a biological fluorescence development reagent, a development method and application of a biological evidence trace, wherein the biological fluorescence development reagent has short reaction time and high safety.
In order to achieve the above purpose, the invention adopts the following technical scheme: the first aspect of the present invention provides a bioluminescence visualization reagent comprising, in weight percent: 0.01% -0.7% of indendione and ZnCl 2 0.005-0.02%, ethyl acetate 1-6%, methanol 1-6%, ethanol 5-20% and CFC 80-92%.
Further, the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.01% -0.4% of indendione and ZnCl 2 0.006-0.009%, ethyl acetate 1-4%, methanol 1-3%, ethanol 5-15% and CFC 83-91%.
Preferably, the volume ratio of ethanol to CFC is 1: 2.5-9.
Further, the volume ratio of ethanol to CFC is 1: 3-8.5.
Still further, the volume ratio of ethanol to CFC is 1:3.5 to 8.5, for example 3.5, 1:4. 1:4.5, 1:5. 1:5.5, 1:6. 1:6.5, 1:7. 1:7.5, 1:8. 1:8.5.
still further, the volume ratio of ethanol to CFC is 1:3.5 to 4.0.
Preferably, the CFC (chlorofluorocarbon) is 1, 2-trichlorotrifluoroethane.
Preferably, the volume ratio of the ethyl acetate to the methanol is 1:0.3 to 3.
Further, the volume ratio of ethyl acetate to methanol is 1:0.4 to 2.5, for example: 1:0.4, 1:0.7, 1:1. 1:1.3, 1:1.5, 1:1.8, 1:2.1, 1:2.4, 1:2.5.
still further, the volume ratio of ethyl acetate to methanol is 1: 2-2.5.
Preferably, the preparation method of the biological fluorescence visualization reagent comprises the following steps: the indendione and ZnCl are mixed 2 Directly mixing ethyl acetate, methanol, ethanol and CFC to obtain the biological fluorescence developing reagent.
In a second aspect, the present invention provides a method for developing a trace of a biological evidence, wherein the object is treated with the biological fluorescent developing reagent, allowed to react at room temperature, and then the trace of the biological evidence is developed under the irradiation of an instrument.
Preferably, the room temperature condition is that the temperature is 5-25 ℃ and the relative humidity is less than 80%.
Further, the room temperature condition is that the temperature is 5-20 ℃ and the relative humidity is less than 78%.
Further, the room temperature condition is that the temperature is 8-15 ℃ and the relative humidity is less than 76%.
Preferably, the reaction time is controlled to be 3-9 min.
Specifically, the reaction time may be adjusted according to the specific object. For example, when the object is a brick, controlling the reaction time to be 5-9 min; when the object is a textile cloth section, controlling the reaction time to be 3-6 min; when the object is white paper, controlling the reaction time to be 1-2 min.
Preferably, the object is soaked, sprayed or dripped with the biological fluorescence visualization reagent.
Preferably, the biophysical trace comprises a fingerprint, which may be a fingerprint or a palmprint.
According to a specific and preferred embodiment, the visualization method is specifically: the object is soaked, sprayed or dripped by the biological fluorescence development reagent, the object reacts for 3-9 min under the conditions that the temperature is 5-25 ℃ and the relative humidity is less than 80%, then green light with the wavelength of 532nm is used for irradiating the detection material for investigation, the surface is controlled to form illumination of 30 kaleidoscope, and the evidence trace is developed under a 540nm filter lens in a photographing mode.
The third aspect of the invention provides an application of a biological fluorescence development reagent in developing a biological evidence trace on a object.
Preferably, the object is one of a rough-surface permeable object and a rough-surface semi-permeable object.
Further, the object comprises one of bricks, textile cloth sections, raw wood, white walls, white paper, kraft paper, toilet paper, invoice documents, leather and screw steel loaded with a coating; further preferably one of brick, textile cloth section and white paper; still more preferably a brick, a woven cloth segment.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
according to the invention, indene diketone is used as a main material, a mixed solution of ethyl acetate and methanol is used as a solution of indene diketone, and a proper amount of zinc chloride and CFC are added, so that the prepared biological fluorescence development reagent can shorten the required reaction time, and meanwhile, a permeable object or a semi-permeable object can be reacted under the condition of room temperature to develop a biological evidence trace. In addition, the biological fluorescence developing reagent can relieve discomfort caused by pungent smell in the use process of operators, and has higher safety and stability.
Drawings
Fig. 1: fingerprint photographs of the bioluminescence visualization reagents on bricks in example 1 and comparative example 1;
fig. 2: fingerprint photographs of the bioluminescence visualization reagents of example 1 and comparative example 1 on the textile fabric sections;
fig. 3: fingerprint photographs of the bioluminescence visualization reagents on bricks in example 1 and comparative example 2;
fig. 4: fingerprint photographs of the bioluminescence development reagents of example 1 and comparative example 2 on the textile fabric sections;
fig. 5: fingerprint photographs of the bioluminescence visualization reagents on bricks in example 1 and comparative example 3;
fig. 6: fingerprint photographs of the bioluminescence development reagents of example 1 and comparative example 3 on the textile fabric sections;
fig. 7: fingerprint photographs of the bioluminescence visualization reagents on the brick in example 1 and comparative example 4;
fig. 8: fingerprint photographs of the bioluminescence development reagents of example 1 and comparative example 4 on the textile fabric sections;
fig. 9: fingerprint photographs of the bioluminescence visualization reagents on the brick in example 2 and comparative example 5;
fig. 10: fingerprint photographs of the bioluminescence visualization reagents on the textile fabric sections in example 2 and comparative example 5;
fig. 11: fingerprint photographs of the bioluminescence visualization reagents on the brick in example 2 and comparative example 6;
fig. 12: fingerprint photographs of the bioluminescence development reagents of example 2 and comparative example 6 on the textile fabric sections;
fig. 13: fingerprint photographs of the bioluminescence visualization reagents on the brick in example 2 and comparative example 7;
fig. 14: fingerprint photographs of the bioluminescence visualization reagents on the textile fabric sections in example 2 and comparative example 7;
fig. 15: fingerprint photographs of the bioluminescence visualization reagents on bricks in example 2 and comparative example 8;
fig. 16: fingerprint photographs of the bioluminescence visualization reagents on the textile fabric sections in example 2 and comparative example 8.
Description of the drawings: in FIGS. 1 to 16, the left graph shows the effect of the bioluminescence in the examples, and the right graph shows the effect of the bioluminescence in the comparative examples.
Detailed Description
All of the features disclosed in this specification, or all of the steps in a method or process disclosed, may be combined in any combination, except for mutually exclusive features or steps.
In view of the shortcomings in the prior art, the inventor of the present invention has long studied and practiced in a large number of ways to propose the technical scheme of the present invention. The technical scheme, the implementation process, the principle and the like are further explained as follows.
In the invention, indene diketone is taken as main material to carry out permeable object or semi-permeable object organismThe display of evidence trace utilizes the reaction of indene diketone and amino acid in sweat, and the produced intermediate product can produce fluorescence display under specific condition (reaction principle is shown as below), at the same time a small quantity of ZnCl is added into the system 2 And the new biological fluorescence development reagent is formed by the synergistic action of methanol, ethyl acetate, ethanol and CFC. The biological fluorescence development reagent has short reaction time with the permeable object or the semi-permeable object, is little influenced by environmental factors such as temperature, humidity and the like, has high safety and stability, and reduces discomfort caused by pungent odor in the use process of operators.
Further, znCl in the bioluminescence visualization reagent of the present invention 2 The content of zinc ions is 0.005-0.02%, preferably 0.006-0.009%, and zinc ions can generate fluorescence under the irradiation of laser to generate synergistic effect with indene diketone, so that the effect of enhancing the appearance can be achieved.
Furthermore, methanol is added in the biological fluorescence developing reagent, and the biological fluorescence developing reagent is mixed with ethyl acetate to act as a dissolver of indene diketone and zinc chloride, and the volume ratio of the ethyl acetate to the methanol is strictly controlled to be 1: in the range of 0.3-3, the solubility of the indene diketone and the zinc chloride in the system is good, the volatility of methanol is higher than that of pure alcohol, the whole volatility of the reagent solution can be improved, meanwhile, a small amount of methanol replaces part of ethyl acetate, the consumption of the ethyl acetate is reduced, the pungent odor of the reagent in the volatilization process is further reduced, and the uncomfortable feeling of staff is further reduced.
Further, CFC is selected as a diluent in the invention, 1, 2-trichlorotrifluoroethane is specifically selected, the use of flammable petroleum ether is avoided, the volatility and the stability of the 1, 2-trichlorotrifluoroethane are stronger than those of petroleum ether and the smell is weaker than those of petroleum ether, the volatility of the reagent is improved, and the volume ratio of ethanol to CFC is strictly controlled to be 1:2.5 to 9, preferably 1: 3.5-8.5; further preferably 1: 3.5-4.0 in ethyl acetate, methanol, znCl 2 Under the cooperation of the components, the reaction time of the reagent is shortened better, the display speed of the biological evidence trace on the surface of the permeable object or the semi-permeable object is accelerated, the safety of the biological display reagent is improved, the content of the pungent odor material is further reduced, and the pungency to staff during the volatilization of the reagent is reduced. In addition, CFCs are more stable than petroleum ether, improving the stability of bioluminescence development reagents.
The invention is further described below with reference to examples. The present invention is not limited to the following examples. The implementation conditions adopted in the embodiments can be further adjusted according to different requirements of specific use, and the implementation conditions which are not noted are conventional conditions in the industry. The technical features of the various embodiments of the present invention may be combined with each other as long as they do not collide with each other.
The experimental methods in the following examples are all conventional methods unless otherwise specified; the experimental materials used, unless specified, are all purchased from conventional biochemical reagent manufacturers.
Example 1
In the embodiment, a quantitative dripping method is adopted, 1mL of biological fluorescence developing reagent is dripped to treat a detection material (specifically a brick with a fingerprint and a textile cloth section), the detection material is stood at room temperature (8 ℃ and humidity of 76%) to enable the detection material to react naturally, green light with the wavelength of 532nm is used for irradiating the detection material to perform investigation, the illuminance of 30 Wanleher Ke is controlled on the surface, and the fingerprint is obtained through a photographing mode under a 540nm filter.
The biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.02g of indendione and 0.01g of ZnCl 2 2mL of ethyl acetate, 5mL of methanol, 10mL of ethanol, and an appropriate amount of 1, 2-trichlorotrifluoroethane (about 83 mL) were fixed to a volume of 100mL.
Comparative example 1
This comparative example is substantially the same as example 1 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.02g of indendione, 2mL of ethyl acetate, 10mL of ethanol, and an appropriate amount of petroleum ether (about 88 mL) were fixed to a volume of 100mL.
The samples were treated with the reagents of example 1 and comparative example 1, respectively, and the fingerprint was shown in FIGS. 1 and 2 (samples: brick, woven cloth sections) after the fingerprint was completely displayed.
For comparison of the development effect, the experiment was performed by using a controlled variable method in which two fingerprints were pressed by the same person under the same conditions on the same kind of objects, one part was treated with an experimental reagent (the reagent in example 1 and example 2), the other part was treated with a comparative reagent (the reagent in comparative examples 1 to 8), the test material was irradiated with green light having a wavelength of 532nm every 30 seconds for investigation, the development starting time and the full development time of the two reagents were recorded by observation, and the improvement rate of the development starting time was calculated. When the finger print is pressed, the hands do not carry out any treatment, and the contact time and the strength are ensured to be consistent as much as possible. Common detection materials are selected in a unified way in the experiment: and (5) bricks and textile cloth sections.
Improvement rate/% = (contrast agent start development time-experimental agent start development time)/contrast agent start development time x 100%
The test results are shown in Table 1.
The difference in development effect between the reagent in comparative example 1 and the reagent in comparative example 1 is a major factor in this set of experiments, and it can be seen from the experimental data that the initial development speed and the complete development speed of the fingerprint in example 1 are faster than those of comparative example 1, and the fingerprint in example 1 does not begin to develop when the tile and the textile fabric section begin to develop.
Comparative example 2
This comparative example is substantially the same as example 1 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.02g of indendione and 0.01g of ZnCl 2 2mL ethyl acetate, 5mL methanol, 10mL ethanol, and an appropriate amount of petroleum ether (about 83 mL) were sized to 100mL.
The samples were treated with the reagents of example 1 and comparative example 2, respectively, and the fingerprint was shown in FIGS. 3 and 4 (samples: brick, woven fabric section) after the fingerprint was completely displayed, and the test results were shown in Table 2.
The effect of CFC and petroleum ether on the development effect was mainly studied in this set of experiments, and it can be seen from the experimental data that the fingerprint in example 1 was developed at a faster starting speed and at a faster full development speed than in comparative example 2. On the tile and textile segment, the fingerprint in example 1 did not begin to appear at the beginning of the tile and textile segment, nor did the fingerprint in comparative example 2.
Comparative example 3
This comparative example is substantially the same as example 1 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.02g of indendione, 2mL of ethyl acetate, 5mL of methanol, 10mL of ethanol, and an appropriate amount of 1, 2-trichlorotrifluoroethane (about 83 mL) were fixed to a volume of 100mL.
The samples were treated with the reagents of example 1 and comparative example 3, respectively, and the fingerprints were shown in FIGS. 5 and 6 (samples: brick, woven fabric section) after the fingerprints were completely displayed, and the test results were shown in Table 3.
The experiments mainly research ZnCl 2 The effect on the development effect, it can be derived from experimental data that the fingerprint in example 1 starts to develop faster than comparative example 3. On the tile, textile fabric section, the fingerprint in example 1 did not yet start to appear at the time the tile and textile fabric section began to appear.
Comparative example 4
This comparative example is substantially the same as example 1 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.02g of indendione and 0.01g of ZnCl 2 2mL of ethyl acetate, 5mL of methanol, 45mL of ethanol, and an appropriate amount of 1, 2-trichlorotrifluoroethane (about 48 mL) were fixed to a volume of 100mL.
The samples were treated with the reagents of example 1 and comparative example 4, respectively, and the fingerprint was shown in FIGS. 7 and 8 (samples: brick, woven fabric section) after the fingerprint was completely displayed, and the test results were shown in Table 4.
The effect of ethanol to CFC ratio on the development effect was mainly studied in this set of experiments, i.e. compared to example 1, the other components of the experimental reagent were not changed in comparative example 4, the ethanol content was increased to 45mL, and the CFC content was reduced to about 48mL. From the experimental data, it can be seen that the fingerprint in example 1 starts to develop faster than comparative example 4. The reagent in comparative example 4 was significantly less volatile than the reagent in example 1, i.e., with the reagent in example 1, when the fingerprint began to appear, the reagent in comparative example 4 had not evaporated completely and the fingerprint did not begin to appear.
Example 2
This embodiment is substantially the same as embodiment 1 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.5g of indendione and 0.01g of ZnCl 2 5mL ethyl acetate, 2mL methanol, 20mL ethanol, and an appropriate amount of 1,2 trichlorotrifluoroethane (about 73 mL) were fixed to a volume of 100mL.
Comparative example 5
This comparative example is substantially the same as example 2 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.5g of indendione, 5mL of ethyl acetate, 10mL of ethanol, and an appropriate amount of petroleum ether (about 85 mL) were fixed to a volume of 100mL.
The samples were treated with the reagents of example 2 and comparative example 5, respectively, and the fingerprint was shown in FIGS. 9 and 10 (samples: brick, woven fabric section) after the fingerprint was completely displayed, and the test results were shown in Table 5.
This set of experiments was performed primarily in comparative example 2 and comparative example 5The agent differs in appearance effect, i.e. ZnCl is added in example 2 2 The influence on the appearance of fingerprints on the detection material after methanol and ethanol consumption are increased and petroleum ether is replaced by CFC. From the experimental data, it can be seen that the fingerprint in example 2 starts to develop faster than comparative example 5. On the tile and textile fabric, the fingerprint in example 2 began to appear while the fingerprint in comparative example 5 did not begin to appear.
Comparative example 6
This comparative example is substantially the same as example 2 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.5g of indendione and 0.01g of ZnCl 2 5mL ethyl acetate, 2mL methanol, 20mL ethanol, and an appropriate amount of petroleum ether (about 73 mL) were sized to 100mL.
The test materials were treated with the reagent of example 2 and the reagent of comparative example 6, respectively, and the photographs of the fingerprints obtained after the fingerprints were completely shown are shown in FIGS. 11 and 12 (test materials: brick, woven cloth), and the test results are shown in Table 6.
This set of experiments studied mainly the effect of CFCs and petroleum ether on the development, i.e. comparative example 6 did not change the other components of the experimental reagent relative to example 2, only CFCs were replaced with petroleum ether. From the experimental data, it can be seen that the fingerprint in example 2 starts to develop faster than comparative example 6. On the tile and textile fabric, the fingerprint in example 2 began to appear while the fingerprint in comparative example 6 did not begin to appear.
Comparative example 7
This comparative example is substantially the same as example 2 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.5g of indendione, 5mL of ethyl acetate, 2mL of methanol, 20mL of ethanol, and an appropriate amount of 1, 2-trichlorotrifluoroethane (about 73 mL) were fixed to a volume of 100mL.
The samples were treated with the reagents of example 2 and comparative example 7, respectively, and the fingerprints were shown in FIGS. 13 and 14 (samples: brick, woven fabric section) after the fingerprints were completely displayed, and the test results were shown in Table 7.
The experiments mainly research ZnCl 2 The effect on the development of the effect, i.e.in comparative example 7, with respect to example 2, the other components of the experimental reagent were not changed, znCl alone 2 And (5) removing. From the experimental data, it can be seen that the fingerprint in example 2 started to develop faster than comparative example 7, and the fingerprint in comparative example 7 did not yet start to develop when the fingerprint in example 2 started to develop on the tile and the textile fabric.
Comparative example 8
This comparative example is substantially the same as example 2 except that: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.5g of indendione and 0.01g of ZnCl 2 5mL ethyl acetate, 2mL methanol, 45mL ethanol, and an appropriate amount of 1, 2-trichlorotrifluoroethane (about 48 mL) were sized to 100mL.
The samples were treated with the reagents of example 2 and comparative example 8, respectively, and the fingerprints were shown in FIGS. 15 and 16 (samples: brick, woven fabric section) after the fingerprints were completely displayed, and the test results were shown in Table 8.
The effect of ethanol to CFC (1, 2-trichlorotrifluoroethane) ratio on the development effect was mainly studied in this set of experiments, i.e. compared with example 2, the ethanol content was increased to 45mL and the CFC content was reduced to about 48mL without changing the other components of the experimental reagent in comparative example 8. From the experimental data, it can be seen that the fingerprint in example 2 started to develop faster than comparative example 8, the reagent in comparative example 8 was significantly less volatile than the reagent in example 2 on the tile, textile fabric, and the reagent in comparative example 8 had not yet evaporated completely when the fingerprint in example 2 started to develop, and the fingerprint had not yet started to develop.
Comparing and observing the experimental reagent 1 with the contrast reagent 1-4, the experimental reagent 2 and the contrast reagent 5-8, and the result shows that the solvent in the indene diketone revealing reagent selects CFC to be superior to petroleum ether; adding trace ZnCl into the reagent 2 The reagent display speed can be increased, and the display effect can be improved; the ratio of ethanol to CFC is controlled, so that the overall volatility of the reagent can be increased, and the reagent display speed, especially on bricks and woven fabrics, is improved. The fingerprint development speed of the experimental reagents 1 and 2 is faster than that of the contrast reagent in comprehensive analysis. And, the fingerprint development effect of the experimental reagents 1 and 2 was equivalent to or even better than that of each comparative example.
The above embodiments are provided to illustrate the technical concept and features of the present invention and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, and are not intended to limit the scope of the present invention. All equivalent changes according to the spirit of the invention should be covered by the protection scope of the invention.
Claims (11)
1. A biological fluorescence visualization reagent, characterized in that: comprises the following components in percentage by weight: 0.01% -0.7% of indendione and ZnCl 2 0.005-0.02%, 1-6% ethyl acetate, 1-6% methanol, 5-20% ethanol and 80-92% CFC; the CFC is 1, 2-trichlorotrifluoroethane.
2. The bioluminescent visualization reagent according to claim 1, wherein: the biological fluorescence visualization reagent comprises the following components in percentage by weight: 0.01% -0.4% of indendione and ZnCl 2 0.006-0.009%, ethyl acetate 1-4%, methanol 1-3%, ethanol 5-15% and CFC 83-91%.
3. The bioluminescent visualization reagent according to claim 1, wherein: the volume ratio of the ethanol to the CFC is 1: 2.5-9.
4. A bioluminescent visualization reagent according to claim 3, wherein: the volume ratio of the ethanol to the CFC is 1: 3-8.5.
5. The bioluminescent visualization reagent according to claim 4, wherein: the volume ratio of the ethanol to the CFC is 1:3.5 to 8.5.
6. The bioluminescent visualization reagent according to claim 1, wherein: the volume ratio of the ethyl acetate to the methanol is 1:0.3 to 3.
7. The bioluminescent visualization reagent according to claim 1, wherein: the volume ratio of the ethyl acetate to the methanol is 1:0.4 to 2.5.
8. The bioluminescent visualization reagent according to claim 1, wherein: the preparation method of the biological fluorescence visualization reagent comprises the following steps: the indendione and ZnCl are mixed 2 Directly mixing ethyl acetate, methanol, ethanol and CFC to obtain the biological fluorescence developing reagent.
9. Use of a bioluminescent visualization reagent according to any one of claims 1 to 8 for visualizing a trace of a biological material evidence on a subject.
10. The use of a bioluminescent visualization reagent according to claim 9 for visualizing a trace of a biological evidence on a subject, wherein: the object is one of a rough-surface permeable object and a rough-surface semi-permeable object.
11. The use of a bioluminescent visualization reagent according to claim 10 for visualizing a trace of a biological evidence on a subject, wherein: the object comprises one of bricks, textile cloth sections, raw wood, white walls, white paper, kraft paper, toilet paper, invoice documents, leather and screw-thread steel loaded with a coating.
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| CN202310772543.4A CN116840204A (en) | 2023-03-23 | 2023-03-23 | Method for displaying trace of biological evidence |
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