CN116064684A - Light bio-based buffer material and preparation method thereof - Google Patents

Light bio-based buffer material and preparation method thereof Download PDF

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CN116064684A
CN116064684A CN202310053129.8A CN202310053129A CN116064684A CN 116064684 A CN116064684 A CN 116064684A CN 202310053129 A CN202310053129 A CN 202310053129A CN 116064684 A CN116064684 A CN 116064684A
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fermentation
bacterial cellulose
buffer material
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应汉杰
陈勇
单军强
刘庆国
温庆仕
余斌
刘桂文
魏荷芬
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Nanjing Institute Of White Biotech Co ltd
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Abstract

The invention discloses a light bio-based buffer material and a preparation method thereof, wherein the light bio-based buffer material is prepared by mixing seed liquid of edible fungi and seed liquid of bacterial cellulose and filling and fermenting; the apparent density of the light bio-based buffer material is 0.1-0.25 g/cm 3 The compression strength is 0.15-0.45 MPa. According to the invention, after mixed bacteria fermentation, the solid matrix and the edible fungus mycelium can be more densely wound and wrapped due to the gel-like state of bacterial cellulose, so that a three-dimensional reticular structure is formed, and the binding force inside the material is effectively improved.

Description

Light bio-based buffer material and preparation method thereof
Technical Field
The invention relates to the field of biological base materials, in particular to a light biological base buffer material and a preparation method thereof.
Background
Currently, various microplastic materials, which are generated due to excessive use and erroneous disposal of non-degradable synthetic plastics, pose a serious threat to human health and natural ecosystems. Microplastic can migrate and accumulate into human tissue, causing inflammation and obstruction of organs. At the same time, they can exist in nature for over 100 years and even hundreds of years, causing profound effects on fresh water and land environments. Taking Polystyrene (PS) and other foaming materials as an example, the plastic is widely used, the annual output can reach 152.7 ten thousand tons, the market scale is 140.9 hundred million yuan, and the plastic is an important plastic pollution source. Meanwhile, the consumption of plastic wastes including express packaging bags or cutlery boxes is nearly ten millions of tons per year. The annual production of plastic products, once a year, is up to 1.2 hundred million tons worldwide, with over 70% being discarded into the soil, air and ocean.
The development of degradable plastics has therefore become a necessary requirement for social development. Compared with the traditional plastic, the bio-based degradable material has remarkable advantages. The straw mycelium material has various advantages as a novel degradable bio-based material, such as integrated formation of microorganism culture, wide sources of straw matrixes, no need of adding toxic chemical adhesives and the like. The current technology for mycelium materials mainly comprises a report of preparation molding technology, and problems caused by some practical applications of the materials are not solved, such as lower mechanical properties, difficult reduction of density and the like of the materials.
Bacterial cellulose is a natural nanocellulose synthesized by microorganisms, and is a biological type material with high rigidity, tensile strength, low density, high purity, biodegradability and easy operation. But there is still less development in the direction of its use. Therefore, the invention provides a light bio-based buffer material and a preparation method thereof.
Disclosure of Invention
The invention aims to: the invention aims to solve the technical problem of providing a light bio-based buffer material aiming at the defects of the prior art.
The invention also solves the technical problem of providing a preparation method of the light bio-based buffer material.
In order to solve the first technical problem, the invention discloses a light bio-based buffer material, which is prepared by mixing seed liquid of edible fungi and seed liquid of bacterial cellulose and filling and fermenting.
In some embodiments, the lightweight biobased buffer material has an apparent density of 0.1 to 0.25g/cm 3 Preferably 0.1 to 0.2g/cm 3 Preferably 0.1 to 0.15g/cm 3 。
In some embodiments, the compressive strength of the lightweight biobased buffer material is 0.15 to 0.45MPa, preferably 0.3 to 0.45MPa, preferably 0.4 to 0.45MPa.
In order to solve the second technical problem, the invention discloses a preparation method of the light bio-based buffer material, which comprises the steps of mixing seed solution of edible fungi with seed solution of bacterial cellulose, filling into a mould, and fermenting; in some embodiments, the preparation method of the light bio-based buffer material comprises mixing seed solution of edible fungi and seed solution of bacterial cellulose, inoculating to solid matrix, filling into mould, solid fermentation, demoulding, drying and dewatering to obtain the light bio-based buffer material.
In some embodiments, the edible fungus is any one or a combination of a plurality of ganoderma lucidum, oyster mushroom, flammulina velutipes, pleurotus eryngii and straw mushroom, preferably ganoderma lucidum and/or oyster mushroom, preferably ganoderma lucidum; the ganoderma lucidum, oyster mushroom, flammulina velutipes, pleurotus eryngii and straw mushroom in the prior art are suitable for the invention and are not limited to specific strains.
In some embodiments, the method for preparing the seed solution of the edible fungi comprises inoculating the edible fungi into an edible fungi seed solution culture medium for fermentation; in some embodiments, the edible fungus seed liquid medium comprises the following composition: nitrogen source 0.5-1.5 g/L, carbon source 10-30 g/L, mgSO 4 0.1～0.9g/L，FeSO 4 ·7H 2 O 0.06～0.3g/L，KH 2 PO 4 0.08-0.8 g/L, VB10.01g/L; in some embodiments, the nitrogen source is an amino acid fermentation waste stream; in some embodiments, the nitrogen source is waste liquid produced after threonine fermentation; in some embodiments, the nitrogen source is threonine fermentation, the fermentation broth obtained after fermentation is concentrated and crystallized by a membrane, and the residual liquid is the nitrogen source; in some embodiments, the temperature of the fermentation is 25 to 30 ℃; in some embodiments, the rotational speed of the fermentation is 120 to 170rpm; in some embodiments, the fermentation time is from 5 to 7 days.
In some embodiments, the bacterial cellulose is acetobacter xylinum, preferably acetobacter xylinum (Acetobacter xylinum) ATCC 700178.
In some embodiments, the bacterial cellulose seed solution is prepared by inoculating bacterial cellulose into a bacterial cellulose seed solution culture medium for fermentation; in some embodiments, the bacterial cellulose seed medium has the following composition: glucose 20-30 g/L, yeast extract 5-15 g/L, peptone 5-15 g/L, caCO 3 0.5-1.5 g/L; preferably glucose 25g/L, yeast extract 10g/L, peptone 10g/L, caCO 3 1g/L; in some embodiments, the temperature of the fermentation is 25 to 35 ℃, preferably 30 ℃; in some embodiments, the rotational speed of the fermentation is 120 to 170rpm, preferably 150rpm; in some embodiments, the fermentation time is 1 to 3 days.
In some embodiments, the volume ratio of the seed solution of the edible fungus to the seed solution of the bacterial cellulose is 2-4:1-3, preferably 3:1-3, preferably 3:2.
In some embodiments, the solid substrate comprises straw, wood chips, and bran; in some embodiments, the solid matrix is composed of the following components in mass fraction: 30-50% of corn straw, 30-50% of wood dust and 10-30% of bran, preferably 40% of corn straw, 40% of wood dust and 20% of bran.
In some embodiments, the seed solution of the edible fungus is mixed with the seed solution of the bacterial cellulose and then inoculated on the solid substrate according to the volume ratio of 10-30%, preferably 15-25%, preferably 20%.
In some embodiments, the temperature of the solid state fermentation is 20 to 30 ℃, preferably 25 ℃; in some embodiments, the solid state fermentation is for a period of 5 to 9 days, preferably 7 days.
In some embodiments, the time for dehydration is 15 to 25 hours, preferably 18 to 22 hours.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
the invention develops a preparation process of the bacterial cellulose culture-mediated mycelium material, so as to assist in improving the mechanical property of the mycelium material, effectively reduce the material density, meet the requirement of light weight, and develop a new direction for the application of the bacterial cellulose.
The invention adopts a mixed fermentation process for mycelium materials for the first time, and the two strains can effectively and cooperatively grow without rejection.
According to the invention, after mixed bacteria fermentation, the solid matrix and the edible fungus mycelium can be more densely wound and wrapped due to the gel-like state of bacterial cellulose, so that a three-dimensional reticular structure is formed, and the binding force inside the material is effectively improved.
Drawings
The foregoing and/or other advantages of the invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings and detailed description.
FIG. 1 is a flow chart of material preparation.
FIG. 2 is a graph of a plate antagonism experiment.
Detailed Description
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials, unless otherwise specified, are commercially available.
Edible fungi (Ganoderma lucidum (Taishan Ganoderma lucidum), oyster mushroom (Sufeng No. 2), pleurotus eryngii (Pleurotus eryngii No. 1), flammulina velutipes (New American No. 15 Flammulina velutipes) and straw mushroom (V901)) were purchased from Tianda edible fungi institute for 7 months in 2022 in the following examples.
The amino acid fermentation waste liquid in the following examples is provided by Ningxia Yipin biotechnology Co., ltd (waste liquid produced after threonine fermentation, see Chinese patent No. 102191290B-threonine for a specific preparation method, in which supernatant liquid in example 3 is subjected to film-passing concentration crystallization, and then the residual liquid is fermentation tail liquid, namely the amino acid fermentation waste liquid in the application).
Compression Performance detection national Standard GB/T8813-2020, apparent Density detection national Standard GB/T6343-2009.
Example 1: preparation of light biobased buffer materials
Two seed solutions are fermented and cultured:
ganoderma lucidum seed liquid: inoculating the outsourced ganoderma lucidum strain into a seed liquid culture medium for fermentation culture, wherein the formula of the seed liquid culture medium is as follows: amino acid fermentation waste liquid 1.1g/L, corn starch 25g/L and MgSO 4 0.4g/L，FeSO 4 ·7H 2 O 0.1g/L，KH 2 PO 4 0.1g/L, VB 1.01 g/L,25 ℃ and 170rpm for about 7 days to obtain seed liquid rich in hypha.
Bacterial cellulose seed solution: acetobacter xylinum (Acetobacter xylinum) ATCC 700178 was inoculated into a seed medium, the formulation of which is as follows: glucose 20g/L, yeast extract 2g/L, peptone 2g/L, caCO 3 0.5g/L,28 ℃ and 100r/min for 2 days to obtain bacterial cellulose seed liquid.
And (3) material inoculation preparation: the obtained ganoderma lucidum seed liquid and bacterial cellulose seed liquid are inoculated to a solid matrix (sterilization treatment is carried out at 121 ℃ for 15min before inoculation) according to 20% of the total volume of the solid matrix (comprising the following components by weight percent of corn straw 40%, wood dust 40% and wheat bran 20%) in an ultra clean bench according to the volume ratio of 3:2, the mixture is filled into a mould after uniform stirring, the mould is sealed and placed in a shaking table at 25 ℃ for static culture for 7 days, and solid fermentation is carried out to prepare the mycelium material. After the solid fermentation is finished, demolding, taking out the straw mycelium material which is shaped and has white mycelium grown on the surface, and placing the material in a 70 ℃ oven for drying and dewatering treatment for 20 hours, so that the aim of inactivating bacteria in a matrix is fulfilled, and the growth of the material is stopped.
The material performance is as follows: detecting the performance of the processed material, wherein the detection result is as follows: apparent density: 0.12g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Compressive strength: 0.43MPa.
Example 2: preparation of light biobased buffer materials
Ganoderma lucidum seed liquid: inoculating the outsourced ganoderma lucidum strain into a seed liquid culture medium for fermentation culture, wherein the formula of the seed liquid culture medium is as follows: amino acid fermentation waste liquid 1.1g/L, corn starch 25g/L and MgSO 4 0.4g/L，FeSO 4 ·7H 2 O 0.1g/L，KH 2 PO 4 0.1g/L, VB 1.01 g/L,25 ℃ and 170rpm for about 7 days to obtain seed liquid rich in hypha.
And (3) material inoculation preparation: inoculating 20% of the total volume of the seed liquid rich in ganoderma lucidum mycelia (comprising the following components in percentage by weight of corn straw 40%, wood dust 40% and bran 20%) to the solid matrix in an ultra-clean bench (sterilization treatment is carried out at 121 ℃ for 15min before inoculation), filling the seed liquid into a mould after uniform stirring, sealing the mould, placing the mould in a shaking table at 25 ℃ for standing culture for 7 days, and carrying out solid fermentation to prepare the mycelium material. After the solid fermentation is finished, demolding, taking out the straw mycelium material which is shaped and has white mycelium grown on the surface, and placing the material in a 70 ℃ oven for drying and dewatering treatment for 20 hours, so that the aim of inactivating bacteria in a matrix is fulfilled, and the growth of the material is stopped.
The material performance is as follows: apparent density: 0.2g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Compressive strength: 0.30MPa.
Example 3: preparation of light biobased buffer materials
Two seed solutions are fermented and cultured:
ganoderma lucidum seed liquid: inoculating outsourced Ganoderma strain into seed liquid culture medium for fermentation culture, wherein the culture medium is prepared byThe formula of the seed liquid culture medium is as follows: amino acid fermentation waste liquid 1.1g/L, corn starch 25g/L, mgSO4 0.4g/L, feSO 4 ·7H 2 O0.1g/L，KH 2 PO4 0.1g/L, VB10.01g/L, 25 ℃ and 170rpm for about 7 days to obtain seed liquid rich in hypha.
Bacterial cellulose seed solution: acetobacter xylinum (Acetobacter xylinum) ATCC 700178 was inoculated into a seed medium, the formulation of which is as follows: glucose 20g/L, yeast extract 2g/L, peptone 2g/L, caCO 3 0.5g/L,28 ℃ and 100r/min for 2 days.
And (3) material inoculation preparation: the obtained ganoderma lucidum seed liquid and bacterial cellulose seed liquid are inoculated to a solid matrix (sterilization treatment is carried out at 121 ℃ for 15min before inoculation) according to 20% of the total volume of the solid matrix (comprising the following components by weight percent of corn straw 40%, wood dust 40% and wheat bran 20%) in an ultra clean bench according to the volume ratio of 4:1, the mixture is filled into a mould after uniform stirring, the mould is sealed and placed in a shaking table at 25 ℃ for static culture for 7 days, and solid fermentation is carried out to prepare the mycelium material. After the solid fermentation is finished, demolding, taking out the straw mycelium material which is shaped and has white mycelium grown on the surface, and placing the material in a 70 ℃ oven for drying and dewatering treatment for 20 hours, so that the aim of inactivating bacteria in a matrix is fulfilled, and the growth of the material is stopped.
The material performance is as follows: detecting the performance of the processed material, wherein the detection result is as follows: apparent density: 0.14g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Compressive strength: 0.4MPa.
Example 4: preparation of light biobased buffer materials
Ganoderma lucidum seed liquid: inoculating the outsourced ganoderma lucidum strain into a seed liquid culture medium for fermentation culture, wherein the formula of the seed liquid culture medium is as follows: amino acid fermentation waste liquid 1.1g/L, corn starch 25g/L and MgSO 4 0.4g/L，FeSO 4 ·7H2O0.1g/L，KH 2 PO 4 0.1g/L, VB 1.01 g/L,25 ℃ and 170rpm for about 7 days to obtain seed liquid rich in hypha.
Bacterial cellulose seed solution: acetobacter xylinum (Acetobacter xylinum) ATCC 700178 was inoculated into a seed medium, the formulation of which is as follows: grapeGlucose 20g/L, yeast extract 2g/L, peptone 2g/L, caCO 3 0.5g/L,28 ℃ and 100r/min for 2 days.
And (3) material inoculation preparation: the obtained ganoderma lucidum seed liquid and bacterial cellulose seed liquid are inoculated to a solid matrix (sterilization treatment is carried out at 121 ℃ for 15min before inoculation) according to the total volume of 20% of the total volume of the solid matrix (comprising the following components by weight percent of corn straw 40%, wood dust 40% and wheat bran 20%) in an ultra clean bench in a volume ratio of 2:3, the mixture is filled into a mould after uniform stirring, the mould is sealed and placed in a shaking table at 25 ℃ for static culture for 7 days, and solid fermentation is carried out to prepare the mycelium material. After the solid fermentation is finished, demolding, taking out the straw mycelium material which is shaped and has white mycelium grown on the surface, and placing the material in a 70 ℃ oven for drying and dewatering treatment for 20 hours, so that the aim of inactivating bacteria in a matrix is fulfilled, and the growth of the material is stopped.
The material performance is as follows: detecting the performance of the processed material, wherein the detection result is as follows: apparent density: 0.13g/cm 3 The method comprises the steps of carrying out a first treatment on the surface of the Compressive strength: 0.35MPa.
Example 5: preparation of light biobased buffer materials
As in example 1, only Ganoderma lucidum was replaced with Pleurotus ostreatus, pleurotus eryngii, flammulina velutipes and straw mushroom respectively, and the properties of the obtained materials are shown in Table 1, and the results show that the compatibility of Ganoderma lucidum is optimal.
TABLE 1
Figure BDA0004059148830000061
Example 6: antagonism experiment
1. Test strain:
ganoderma lucidum, acetobacter xylinum ATCC 700178
2. Reagent consumable
500mLPDA solid culture medium, 12 sterile oxford cups, mark pen, ruler and 26 ℃ constant temperature incubator
3. Seed formulation
Solid medium: PDA, pH is natural
4. Culture conditions
Sterilization conditions: 121 ℃,15 minutes;
culture conditions: culturing at 26 deg.C for 2-7 days.
5. Test method
Ganoderma lucidum and ATCC 700178 were cut out on PDA medium with oxford cup (diameter 5 mm), and the cells were transferred to both ends of PDA medium plate (diameter 9 cm) with the cell surface facing downwards, and cultured at 26℃for 2-7d with a distance of 5cm between them, and the colony radius was measured between every two cells. The test was repeated 3 times with only the single bacteria as a control.
6. Experimental evaluation
As shown in FIG. 2, the colonies did not significantly inhibit the growth of the indicator bacteria, and the phenomenon of mutual inhibition of growth like a transverse septum/inhibition zone (disjoint), a bulge, an overlap (intersecting) and the like was not formed.
The invention provides a light bio-based buffer material, a method for preparing the same, and a method for realizing the technical scheme, wherein the method and the method are a plurality of methods, the method is only a preferred embodiment of the invention, and it should be noted that a plurality of improvements and modifications can be made by one of ordinary skill in the art without departing from the principle of the invention, and the improvements and modifications are also considered as the protection scope of the invention. The components not explicitly described in this embodiment can be implemented by using the prior art.

Claims (12)

1. A light bio-based buffer material is characterized in that the buffer material is prepared by mixing seed liquid of edible fungi and seed liquid of bacterial cellulose and filling and fermenting;
the apparent density of the light bio-based buffer material is 0.1-0.25 g/cm 3 The compression strength is 0.15-0.45 MPa.
2. The lightweight biobased buffer material of claim 1, wherein the lightweight biobased buffer material has an apparent density of 0.1 to 0.2g/cm 3 Preferably 0.1 to 0.15g/cm 3 。
3. The lightweight biobased buffer material of claim 1, wherein the compressive strength of the lightweight biobased buffer material is 0.3-0.45 MPa, preferably 0.4-0.45 MPa.
4. A method for producing a lightweight bio-based buffer material according to any one of claims 1 to 3, wherein the seed solution of edible fungi and the seed solution of bacterial cellulose are mixed and then subjected to fermentation in a filling mold.
5. The preparation method of claim 4, wherein the light bio-based buffer material is obtained by mixing the seed solution of the edible fungi with the seed solution of the bacterial cellulose, inoculating to a solid matrix, filling a mould, performing solid fermentation, demolding, and drying and dehydrating.
6. The preparation method according to claim 5, wherein the edible fungus is any one or a combination of a plurality of ganoderma lucidum, oyster mushroom, flammulina velutipes, pleurotus eryngii and straw mushroom, preferably ganoderma lucidum and/or oyster mushroom, preferably ganoderma lucidum;
preferably, the preparation method of the edible fungi seed liquid comprises inoculating edible fungi into edible fungi seed liquid culture medium for fermentation;
preferably, the edible fungus seed liquid culture medium comprises the following components: nitrogen source 0.5-1.5 g/L, carbon source 10-30 g/L, mgSO 4 0.1～0.9g/L，FeSO 4 ·7H 2 O 0.06～0.3g/L，KH 2 PO 4 0.08～0.8g/L，VB1 0.01g/L；
Preferably, the temperature of the fermentation is 25-30 ℃; preferably, the rotation speed of the fermentation is 120-170 rpm; preferably, the fermentation time is 5 to 7 days.
7. The preparation method according to claim 5, wherein the bacterial cellulose is acetobacter xylinum, preferably acetobacter xylinum (Acetobacter xylinum) ATCC 700178;
preferably, the preparation method of the bacterial cellulose seed solution comprises the steps of inoculating bacterial cellulose into a bacterial cellulose seed solution culture medium for fermentation;
preferably, the bacterial cellulose seed medium has the following composition: glucose 20-30 g/L, yeast extract 5-15 g/L, peptone 5-15 g/L, caCO 3 0.5-1.5 g/L; preferably glucose 25g/L, yeast extract 10g/L, peptone 10g/L, caCO 3 1g/L；
Preferably, the temperature of the fermentation is 25-35 ℃, preferably 30 ℃; preferably, the rotational speed of the fermentation is 120-170 rpm, preferably 150rpm; preferably, the fermentation time is 1 to 3 days.
8. The method according to claim 5, wherein the volume ratio of the seed solution of the edible fungi to the seed solution of the bacterial cellulose is 2-4:1-3, preferably 3:1-3, preferably 3:2.
9. The method of claim 5, wherein the solid substrate comprises straw, wood chips, and bran; preferably, the solid matrix consists of the following components in mass fraction: 30-50% of corn straw, 30-50% of wood dust and 10-30% of bran, preferably 40% of corn straw, 40% of wood dust and 20% of bran.
10. The method according to claim 5, wherein the seed solution of the edible fungi is mixed with the seed solution of the bacterial cellulose and inoculated to the solid substrate in a volume ratio of 10 to 30%, preferably 15 to 25%, preferably 20%.
11. The method according to claim 5, wherein the solid state fermentation temperature is 20-30 ℃, preferably 25 ℃; preferably, the solid state fermentation is carried out for a period of 5 to 9 days, preferably 7 days.
12. The process according to claim 5, wherein the dehydration time is 15 to 25 hours, preferably 18 to 22 hours.
CN202310053129.8A 2023-02-03 2023-02-03 Light bio-based buffer material and preparation method thereof Pending CN116064684A (en)

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