CN116059440B - Bionic muscle material with anisotropy and preparation method thereof - Google Patents
Bionic muscle material with anisotropy and preparation method thereof Download PDFInfo
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- CN116059440B CN116059440B CN202310112874.5A CN202310112874A CN116059440B CN 116059440 B CN116059440 B CN 116059440B CN 202310112874 A CN202310112874 A CN 202310112874A CN 116059440 B CN116059440 B CN 116059440B
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- 210000003205 muscle Anatomy 0.000 title claims abstract description 38
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 38
- 239000000463 material Substances 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 114
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 114
- 239000000243 solution Substances 0.000 claims abstract description 84
- 239000000017 hydrogel Substances 0.000 claims abstract description 65
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 44
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 44
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 44
- 239000000835 fiber Substances 0.000 claims abstract description 43
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000001112 coagulating effect Effects 0.000 claims abstract description 19
- 230000015271 coagulation Effects 0.000 claims abstract description 19
- 238000005345 coagulation Methods 0.000 claims abstract description 19
- 238000002347 injection Methods 0.000 claims abstract description 19
- 239000007924 injection Substances 0.000 claims abstract description 19
- 238000002791 soaking Methods 0.000 claims abstract description 15
- 238000011068 loading method Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000008367 deionised water Substances 0.000 claims description 23
- 229910021641 deionized water Inorganic materials 0.000 claims description 23
- 238000002156 mixing Methods 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 238000001125 extrusion Methods 0.000 claims description 7
- 230000003592 biomimetic effect Effects 0.000 claims description 2
- 238000011010 flushing procedure Methods 0.000 abstract description 6
- 238000010257 thawing Methods 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
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- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000012407 engineering method Methods 0.000 description 3
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- 238000002425 crystallisation Methods 0.000 description 1
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- 230000005684 electric field Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/30—Materials or treatment for tissue regeneration for muscle reconstruction
Abstract
The invention provides a bionic muscle material with anisotropy and a preparation method thereof, wherein the method comprises the following steps: preparing a polyvinyl alcohol solution; preparing an ammonium sulfate coagulation bath solution; loading a polyvinyl alcohol solution into a syringe, and then mounting the syringe on a syringe pump; placing a syringe pump at one end of the coagulation tank, and immersing the needle point of the syringe into the ammonium sulfate solution; the collecting shaft is arranged at the other end of the coagulating basin, and the height is adjusted to facilitate the collection of the polyvinyl alcohol fibers; starting a collecting shaft and an injection pump in sequence to collect fibers; and after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking the anisotropic hydrogel in an ammonium sulfate solution, and finally flushing to obtain the bionic muscle hydrogel. The preparation method is simple, the time for the polyvinyl alcohol solution to enter the coagulating bath is short, and the polyvinyl alcohol fiber can be bonded into the bionic muscle hydrogel with anisotropy through simple assembly, so that the mechanical property is good.
Description
Technical Field
The invention relates to a bionic muscle material with anisotropy and a preparation method thereof, belonging to the field of bionic materials.
Background
Biological tissues generally exhibit good anisotropic mechanical properties due to their well-developed microstructure. The current structural engineering methods for creating anisotropic structures in hydrogels are mainly freeze casting, mechanical stretching and compounding, where directional freeze thawing is widely used due to its versatility for various polymers. However, oriented freeze-thaw hydrogels with micro-orientations generally exhibit weaker mechanical properties. The strength and fracture toughness of mechanically trained hydrogels and composite hydrogels incorporating micro/nano-sized fiber reinforcement are significantly improved over homogeneous hydrogels, but also have limited extensibility. These structural engineering methods focus on optimizing the micro/nano structure of existing hydrogels, however, creating strong, stretchable and fatigue resistant hydrogels with finer hierarchical structures while using general simple methods remains a challenge.
Bionic muscle materials, on the other hand, often require the ability to convert a stimulus response into a mechanical response, i.e. driving performance. These stimuli include heat, light, magnetic fields, electric fields, and the like. Numerous reports detail the method of converting stimulus input into a mechanical response, including immobilization of micro/nanostructures on stimulus-responsive materials, the micro/nanostructures being made of stimulus-responsive materials, and the driving induced by shape changes of the underlying micro/nanostructure material, but most have problems of complex synthesis and low driving stress.
Summarizing, the drawbacks of the prior art are mainly: 1) The current structural engineering methods for preparing anisotropic hydrogels are relatively complex to operate. 2) Most of the bionic muscle fibers studied at present have low mechanical strength. 3) At present, the main stream bionic muscle fiber has no good driving mechanism.
Disclosure of Invention
The invention aims at solving the problems in the prior art and provides a bionic muscle material with anisotropy and a preparation method thereof, wherein the bionic muscle material is prepared by using the Hofmeister effect to change the polymerization state of a polyvinyl alcohol solution by simply adding specific ions, so that the polyvinyl alcohol fiber is obtained by polymerization and crystallization. Because of the short time for the polyvinyl alcohol solution to enter the coagulation bath, the polyvinyl alcohol fibers can be bonded together to form an anisotropic hydrogel with orientation.
The Hofmeister effect shall also be called ion-specific effect, which refers to the phenomenon that polymers are precipitated from water in the presence of salts.
The technical scheme of the invention is as follows:
a bionic muscle material with anisotropy and a preparation method thereof comprise the following steps:
preparing a polyvinyl alcohol solution;
preparing a coagulating bath ammonium sulfate solution;
loading the polyvinyl alcohol solution into a syringe, and mounting the syringe on a syringe pump;
placing the injection pump at one end of a coagulation tank, immersing the needle point of the injector into an ammonium sulfate solution, and extruding the polyvinyl alcohol solution through the injection pump to obtain polyvinyl alcohol fibers;
placing a collecting shaft at the other end of the coagulating basin, and collecting the polyvinyl alcohol fibers by rotating the collecting shaft, so that the polyvinyl alcohol fibers are wound on the collecting shaft and form hydrogel;
and after the polyvinyl alcohol solution in the injector is extruded, taking down the hydrogel formed on the collecting shaft, and soaking the hydrogel in an ammonium sulfate solution to obtain the bionic muscle hydrogel material with anisotropy.
Further, the preparing of the polyvinyl alcohol solution includes:
polyvinyl alcohol and deionized water are used as raw materials to prepare a polyvinyl alcohol solution, which comprises the following concrete steps: 8-10 parts by weight of polyvinyl alcohol and 90-92 parts by weight of deionized water are taken, mixed, heated and stirred for 0.5-1h at the temperature of 80-100 ℃ to completely dissolve polyvinyl alcohol solid particles, and a uniform polyvinyl alcohol solution is obtained.
Further, the preparing of the coagulation bath ammonium sulfate solution includes:
the method for preparing the coagulating bath ammonium sulfate solution by taking ammonium sulfate and deionized water as raw materials comprises the following specific steps: mixing 30-40 parts by weight of ammonium sulfate and 60-70 parts by weight of deionized water to obtain a coagulating bath ammonium sulfate solution.
Further, the polyvinyl alcohol solution is filled into a syringe using a 5-20mL syringe.
Further, the extrusion speed of the injection pump is 0.2-0.8mL/min.
Further, the speed of the collecting shaft is 10-40rpm.
Further, collecting the polyvinyl alcohol fibers by rotating the collecting shaft includes:
the collecting shaft and the injection pump are started in sequence, and the polyvinyl alcohol fiber is pulled onto the collecting shaft by forceps at first, so that the fiber is stably wound on the collecting shaft.
Further, the hydrogel removed from the collecting shaft is soaked in a 20-40wt% ammonium sulfate solution for 1-24 hours.
A bionic muscle material with anisotropy is prepared by the preparation method of the bionic muscle material with anisotropy.
Accordingly, the present invention provides the following effects and/or advantages:
firstly, the preparation method is simple, and the polymerization state of the polyvinyl alcohol prepolymer solution is changed by simply adding specific ions, so that the polyvinyl alcohol prepolymer solution is precipitated, and thus the polyvinyl alcohol fiber is obtained; secondly, the time for the polyvinyl alcohol solution to enter the coagulating bath is short, and the polyvinyl alcohol fiber can be bonded into bionic muscle hydrogel through simple assembly; thirdly, the mechanical property is good, and the tensile stress can reach 7.75MPa.
It is to be understood that both the foregoing general description and the following detailed description of the present invention are exemplary and explanatory and are intended to provide further explanation of the invention as claimed.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of the preparation process of the present invention.
FIG. 2 is a schematic representation of the preparation of anisotropic biomimetic muscle hydrogels.
FIG. 3 is a graphical representation of tensile property test data for different orientations of polyvinyl alcohol hydrogels.
FIG. 4 is a graph showing the comparison of mechanical properties of polyvinyl alcohol hydrogels obtained by different preparation methods.
FIG. 5 is a graph showing the crystallinity contrast data of polyvinyl alcohol hydrogels obtained by different preparation methods.
Detailed Description
For the convenience of understanding of those skilled in the art, the technical scheme of the present invention will be described in further detail with reference to the accompanying drawings: it should be understood that, in this embodiment, the steps mentioned in this embodiment may be performed sequentially or sequentially, or may be performed simultaneously or partially, unless specifically stated otherwise. It will be apparent that the described embodiments are some, but not all, of the embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
In this embodiment, the terms "first", "second", and the like, as used herein, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
The specific embodiments of the present invention are as follows:
a method for preparing a bionic muscle material with anisotropy, comprising the following steps:
(1) Mixing 8-10 parts by weight of polyvinyl alcohol and 90-92 parts by weight of deionized water, and heating and stirring at 80-100 ℃ for 0.5-1h to completely dissolve polyvinyl alcohol solid particles, thereby obtaining a uniform polyvinyl alcohol solution;
(2) Mixing 30-40 parts by weight of ammonium sulfate and 60-70 parts by weight of deionized water to obtain an ammonium sulfate coagulation bath solution;
(3) Filling a polyvinyl alcohol solution into a 5-20mL syringe, and then mounting the syringe on a syringe pump;
(4) Placing a syringe pump at one end of a coagulation tank, immersing a needle point of the syringe in an ammonium sulfate solution, and setting the extrusion speed of the syringe pump to be 0.2-0.8mL/min;
(5) Placing a collecting shaft at the other end of the coagulating basin, adjusting the height to conveniently collect the polyvinyl alcohol fibers, and setting the speed of the collecting shaft to be 10-40rpm;
(6) Starting a collecting shaft and an injection pump in sequence, and pulling the polyvinyl alcohol fiber onto the collecting shaft by using tweezers at first to enable the fiber to be stably wound on the collecting shaft;
(7) And after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking and flushing the anisotropic hydrogel in deionized water, and then soaking the anisotropic hydrogel in 20-40wt% ammonium sulfate solution for 1-24 hours to obtain the bionic muscle hydrogel with anisotropy.
A bionic muscle material with anisotropy is prepared by the preparation method of the bionic muscle material with anisotropy.
Example 1
(1) Mixing 10 parts by weight of polyvinyl alcohol and 90 parts by weight of deionized water, and heating and stirring at 90 ℃ for 1h to completely dissolve polyvinyl alcohol solid particles to obtain 10wt% polyvinyl alcohol solution;
(2) Mixing 40 parts by weight of ammonium sulfate and 60 parts by weight of deionized water to obtain a 40wt% coagulating bath ammonium sulfate solution;
(3) Filling a 10mL syringe with a polyvinyl alcohol solution, and mounting the syringe on a syringe pump;
(4) Placing a syringe pump at one end of a coagulation tank, immersing a needle point of the syringe pump into an ammonium sulfate solution, and setting the extrusion speed of the syringe pump to be 0.6mL/min;
(5) Placing a collecting shaft at the other end of the coagulating basin, adjusting the height to conveniently collect the polyvinyl alcohol fibers, and setting the rotating speed of the collecting shaft to be 25rpm;
(6) Starting a collecting shaft and an injection pump in sequence, and pulling the polyvinyl alcohol fiber onto the collecting shaft by using tweezers at first to enable the fiber to be stably wound on the collecting shaft;
(7) And after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking and flushing the anisotropic hydrogel in deionized water, and then soaking the anisotropic hydrogel in 40wt% ammonium sulfate solution for 24 hours to obtain the bionic muscle hydrogel.
Referring to the preparation process flow chart of fig. 1, a polyvinyl alcohol solution and an ammonium sulfate solution of a coagulation bath are prepared respectively, the polyvinyl alcohol solution is extruded into a coagulation tank containing the ammonium sulfate solution through a syringe, the polymerization state of the polyvinyl alcohol solution is changed by simply adding specific ions, so that the polyvinyl alcohol fibers are obtained by precipitation, the obtained polyvinyl alcohol fibers are collected by a collecting shaft, the time for the polyvinyl alcohol solution to enter the coagulation bath is short, the polyvinyl alcohol fibers can be bonded into hydrogel through simple assembly, and the hydrogel is further soaked in the ammonium sulfate solution, so that the bionic muscle hydrogel material with anisotropy is prepared.
FIG. 2 is a schematic diagram showing the preparation of anisotropic bionic muscle hydrogel, in which a syringe pump is disposed at one end of a square coagulation tank, a collecting shaft is disposed at the other end, polyvinyl alcohol is extruded from the syringe and forms polyvinyl alcohol fibers under the action of a coagulating bath ammonium sulfate solution, the polyvinyl alcohol fibers are pulled onto the collecting shaft by forceps, the speeds of the syringe pump and the collecting shaft are controlled, the polyvinyl alcohol fibers obtained through coagulating bath treatment are continuously wound on the collecting shaft, and the polyvinyl alcohol fibers on the collecting shaft are adhered by simple assembly to form the anisotropic hydrogel, and the hydrogel has a structure similar to muscles and is the bionic muscle hydrogel.
Fig. 3 is a schematic diagram of tensile property test data of different orientations of the polyvinyl alcohol hydrogel, and it can be seen that the polyvinyl alcohol hydrogel has anisotropy, good mechanical properties, and a tensile stress parallel to the fiber orientation of 7.75MPa.
Example 2
(1) Mixing 8 parts by weight of polyvinyl alcohol and 92 parts by weight of deionized water, and heating and stirring for 1h at 90 ℃ to completely dissolve polyvinyl alcohol solid particles to obtain 8wt% polyvinyl alcohol solution;
(2) Mixing 30 parts by weight of ammonium sulfate and 70 parts by weight of deionized water to obtain a 30wt% ammonium sulfate coagulation bath solution;
(3) Filling a 10mL syringe with a polyvinyl alcohol solution, and mounting the syringe on a syringe pump;
(4) Placing a syringe pump at one end of a coagulation tank, immersing a needle point of the syringe in the ammonium sulfate solution, and setting the extrusion speed of the syringe pump to be 0.6mL/min;
(5) Placing a collecting shaft at the other end of the coagulating basin, adjusting the height to conveniently collect the polyvinyl alcohol fibers, and setting the speed of the collecting shaft to be 25rpm;
(6) Starting a collecting shaft and an injection pump in sequence, and pulling the polyvinyl alcohol fiber onto the collecting shaft by using tweezers at first to enable the fiber to be stably wound on the collecting shaft;
(7) And after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking and flushing the anisotropic hydrogel in deionized water, and then soaking the anisotropic hydrogel in a 20wt% ammonium sulfate solution for 10 hours to obtain the bionic muscle hydrogel.
Example 3
(1) 9 parts by weight of polyvinyl alcohol and 91 parts by weight of deionized water are taken, mixed, heated and stirred at 90 ℃ for 0.5h to completely dissolve polyvinyl alcohol solid particles, and 9wt% polyvinyl alcohol solution is obtained;
(2) Mixing 35 parts by weight of ammonium sulfate and 65 parts by weight of deionized water to obtain 35wt% ammonium sulfate coagulation bath solution;
(3) Filling a 10mL syringe with a polyvinyl alcohol solution, and mounting the syringe on a syringe pump;
(4) Placing a syringe pump at one end of a coagulation tank, immersing a needle point of the syringe in an ammonium sulfate solution, and setting the extrusion speed of the syringe pump to be 0.8mL/min;
(5) Placing a collecting shaft at the other end of the coagulating basin, adjusting the height to conveniently collect the polyvinyl alcohol fibers, and setting the speed of the collecting shaft to be 40rpm;
(6) Starting a collecting shaft and an injection pump in sequence, and pulling the polyvinyl alcohol fiber onto the collecting shaft by using tweezers at first to enable the fiber to be stably wound on the collecting shaft;
(7) And after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking and flushing the anisotropic hydrogel in deionized water, and then soaking the anisotropic hydrogel in 30wt% ammonium sulfate solution for 15 hours to obtain the bionic muscle hydrogel.
Example 4
(1) Mixing 10 parts by weight of polyvinyl alcohol and 90 parts by weight of deionized water, and heating and stirring at 90 ℃ for 1h to completely dissolve polyvinyl alcohol solid particles to obtain 10wt% polyvinyl alcohol solution;
(2) Mixing 40 parts by weight of ammonium sulfate and 60 parts by weight of deionized water to obtain a 40wt% coagulating bath ammonium sulfate solution;
(3) Filling a 10mL syringe with a polyvinyl alcohol solution, and mounting the syringe on a syringe pump;
(4) Placing a syringe pump at one end of a coagulation tank, immersing a needle point of the syringe pump into an ammonium sulfate solution, and setting the extrusion speed of the syringe pump to be 0.6mL/min;
(5) Placing a collecting shaft at the other end of the coagulating basin, adjusting the height to conveniently collect the polyvinyl alcohol fibers, and setting the rotating speed of the collecting shaft to be 25rpm;
(6) Starting a collecting shaft and an injection pump in sequence, and pulling the polyvinyl alcohol fiber onto the collecting shaft by using tweezers at first to enable the fiber to be stably wound on the collecting shaft;
(7) And after the injection of the polyvinyl alcohol solution in the injector is finished, taking down the anisotropic hydrogel formed on the collecting shaft, soaking and flushing the anisotropic hydrogel in deionized water, and then soaking the anisotropic hydrogel in 40wt% ammonium sulfate solution for 2 hours to obtain the bionic muscle hydrogel.
Comparative example 1
The polyvinyl alcohol hydrogel is prepared by adopting a freeze thawing method:
(1) Mixing 10 parts by weight of polyvinyl alcohol and 90 parts by weight of deionized water, and heating and stirring for 1h at 90 ℃ to completely dissolve polyvinyl alcohol solid particles to obtain a uniform polyvinyl alcohol solution;
(2) Injecting the polyvinyl alcohol solution into a polytetrafluoroethylene mould, and then placing the mould into a refrigerator at the temperature of minus 20 ℃ for storage for 12 hours;
(3) Taking out the polytetrafluoroethylene mould containing the polyvinyl alcohol, and thawing for 8 hours at room temperature to obtain the polyvinyl alcohol hydrogel formed by freeze thawing.
Comparative example 2
The polyvinyl alcohol hydrogel is prepared by adopting a method of freezing and thawing and soaking salt:
(1) Mixing 10 parts by weight of polyvinyl alcohol and 90 parts by weight of deionized water, and heating and stirring for 1h at 90 ℃ to completely dissolve polyvinyl alcohol solid particles to obtain a uniform polyvinyl alcohol solution;
(2) Injecting the polyvinyl alcohol solution into a polytetrafluoroethylene mould, and then placing the mould into a refrigerator at the temperature of minus 20 ℃ for storage for 12 hours;
taking out the polytetrafluoroethylene mould containing the polyvinyl alcohol, taking out the frozen polyvinyl alcohol, and putting the frozen polyvinyl alcohol into a 40wt% ammonium sulfate solution for soaking for 24 hours to obtain the polyvinyl alcohol hydrogel with freeze thawing and foaming salt.
Fig. 4-5 are respectively comparative graphs of mechanical properties and crystallinity of polyvinyl alcohol hydrogels obtained by different preparation methods: it can be seen that the anisotropic hydrogel prepared in example 4 has significantly superior mechanical properties and crystallinity compared to the hydrogel prepared in comparative example 1 by freeze thawing and the hydrogel prepared in comparative example 2 by freeze thawing plus bubble salt.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A method for preparing a bionic muscle material with anisotropy, comprising the following steps:
preparing a polyvinyl alcohol solution;
preparing a coagulating bath ammonium sulfate solution;
loading the polyvinyl alcohol solution into a syringe, and mounting the syringe on a syringe pump;
placing the injection pump at one end of a coagulation tank, immersing the needle point of the injector into an ammonium sulfate solution, and extruding the polyvinyl alcohol solution through the injection pump to obtain polyvinyl alcohol fibers;
placing a collecting shaft at the other end of the coagulating basin, and collecting the polyvinyl alcohol fibers by rotating the collecting shaft, so that the polyvinyl alcohol fibers are wound on the collecting shaft and form hydrogel;
and after the polyvinyl alcohol solution in the injector is extruded, taking down the hydrogel formed on the collecting shaft, and soaking the hydrogel in an ammonium sulfate solution to obtain the bionic muscle hydrogel material with anisotropy.
2. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the preparation of the polyvinyl alcohol solution comprises the following steps:
8-10 parts by weight of polyvinyl alcohol and 90-92 parts by weight of deionized water are taken, mixed, heated and stirred for 0.5-1h at the temperature of 80-100 ℃ to completely dissolve polyvinyl alcohol solid particles, and a uniform polyvinyl alcohol solution is obtained.
3. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the preparing of the coagulation bath ammonium sulfate solution comprises the following steps:
mixing 30-40 parts by weight of ammonium sulfate and 60-70 parts by weight of deionized water to obtain a coagulating bath ammonium sulfate solution.
4. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the polyvinyl alcohol solution was filled into a 5-20mL syringe.
5. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the extrusion speed of the injection pump is 0.2-0.8mL/min.
6. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the rotational speed of the collection shaft is 10-40rpm.
7. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: collecting the polyvinyl alcohol fibers by rotating the collection shaft includes:
the polyvinyl alcohol fibers are drawn onto a collection shaft, the fibers are stably wound on the collection shaft, and the collection shaft is rotated.
8. The method for preparing the bionic muscle material with anisotropy according to claim 1, wherein the method comprises the following steps: the hydrogel taken off the collecting shaft is soaked in an ammonium sulfate solution with the weight percent of 20-40 percent for 1-24 hours.
9. A biomimetic muscle material with anisotropy, characterized in that: is prepared by a method for preparing the bionic muscle material having anisotropy according to any one of claims 1 to 8.
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