CN116046645A - Blood smear scanning method, film reader and storage medium - Google Patents
Blood smear scanning method, film reader and storage medium Download PDFInfo
- Publication number
- CN116046645A CN116046645A CN202211618371.7A CN202211618371A CN116046645A CN 116046645 A CN116046645 A CN 116046645A CN 202211618371 A CN202211618371 A CN 202211618371A CN 116046645 A CN116046645 A CN 116046645A
- Authority
- CN
- China
- Prior art keywords
- image
- blood smear
- region
- magnification
- microscope device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 203
- 239000008280 blood Substances 0.000 title claims abstract description 203
- 238000000034 method Methods 0.000 title claims abstract description 70
- 210000004027 cell Anatomy 0.000 claims description 138
- 210000003743 erythrocyte Anatomy 0.000 claims description 45
- 238000003384 imaging method Methods 0.000 claims description 40
- 238000004590 computer program Methods 0.000 claims description 8
- 238000004891 communication Methods 0.000 claims description 5
- 210000000265 leukocyte Anatomy 0.000 claims description 5
- 230000000007 visual effect Effects 0.000 claims description 3
- 238000010586 diagram Methods 0.000 description 7
- 238000013528 artificial neural network Methods 0.000 description 6
- 230000002159 abnormal effect Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 238000003062 neural network model Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 238000013527 convolutional neural network Methods 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 210000000948 non-nucleated cell Anatomy 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000001537 neural effect Effects 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 210000003924 normoblast Anatomy 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The embodiment of the invention provides a blood smear scanning method, a film reader and a storage medium, and belongs to the field of film readers. The method comprises the following steps: controlling a microscope device to scan the blood smear based on a first magnification, and stitching a plurality of first images obtained by scanning to obtain a first stitched image; determining a first image area and a second image area in the first spliced image; identifying and marking cells of a first type in a first image area and identifying and marking cells of a second type in a second image area to obtain a target image; and according to the target image, controlling the microscope device to scan the blood smear based on the second magnification to obtain a plurality of cell images. The technical scheme of the embodiment of the invention improves the accuracy of blood smear scanning.
Description
Technical Field
The invention relates to the field of film readers, in particular to a blood smear scanning method, a film reader and a storage medium.
Background
At present, in order to assist a doctor in diagnosing a patient, a series of examinations are required to be performed on blood of the patient, mainly, a blood smear is made by using the blood of the patient, and a scanning machine scans the boundary of the body tail in the blood smear, so that a scanning image of the boundary of the body tail is obtained, and cell identification and marking are performed on the scanning image, so that an inspector can read the blood smear through the image marked with cells. However, the presenting modes of cells at different positions on the blood smear are different, but the existing blood smear scanning method mainly scans the tail junction of the blood smear fixedly, but some cells overlap or aggregate at the tail junction, and the positions of abnormal cells on different blood smears are also different, so that abnormal cells are easy to miss, and the scanning accuracy of the blood smear cannot be ensured.
Disclosure of Invention
The embodiment of the invention provides a blood smear scanning method, a film reader and a storage medium, aiming at improving the scanning accuracy of blood smears.
In a first aspect, an embodiment of the present invention provides a blood smear scanning method, including: controlling a microscope device to scan the blood smear based on a first magnification, and stitching a plurality of first images obtained by scanning to obtain a first stitched image; determining a first image area and a second image area in the first spliced image, wherein the first image area is an imaging area corresponding to a body tail juncture area of the blood smear, and the second image area comprises an imaging area corresponding to a tail area of the blood smear and/or an imaging area corresponding to a coastline area; identifying and marking cells of a first type in the first image area and identifying and marking cells of a second type in the second image area to obtain a target image; and controlling the microscope device to scan the blood smear based on the second magnification according to the target image to obtain a plurality of cell images.
In a second aspect, an embodiment of the present invention further provides a film reader, the film reader including a microscope device, a processor, a memory, a computer program stored on the memory and executable by the processor, and a data bus for implementing a connection communication between the processor and the memory, wherein the computer program, when executed by the processor, implements the blood smear scanning method according to the first aspect.
In a third aspect, embodiments of the present invention further provide a storage medium for computer-readable storage, the storage medium storing one or more programs executable by one or more processors to implement the blood smear scanning method according to the first aspect.
The embodiment of the invention provides a blood smear scanning method, a smear reader and a storage medium, wherein a microscope device is controlled to scan a blood smear by using a small magnification, a plurality of images obtained by scanning are spliced to obtain an image containing more cells, the smear reader recognizes and marks different types of cells aiming at an imaging area corresponding to a tail boundary area and at least one imaging area corresponding to a tail area and a coastline area in the image to obtain a target image, so that omission of abnormal cells can be avoided, the microscope device is controlled to scan the blood smear by using a large magnification according to the target image, different types of cells on the blood smear can be accurately scanned, and the comprehensiveness and accuracy of blood smear scanning are greatly improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic flow chart of a blood smear scanning method according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an overlapping area between two adjacent first images according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of stitching two adjacent first images according to an embodiment of the present invention;
FIG. 4 is a flow chart illustrating a sub-step of the blood smear scanning method of FIG. 1;
FIG. 5 is a schematic view of the head, body and tail of a blood smear in an embodiment of the invention;
FIG. 6 is a schematic illustration of a middle region of a blood smear in an embodiment of the invention;
FIG. 7 is a flow chart illustrating another sub-step of the blood smear scanning method of FIG. 1;
FIG. 8 is a schematic diagram of a target image in an embodiment of the invention;
FIG. 9 is a schematic representation of a plurality of cell images in an embodiment of the invention;
fig. 10 is a schematic block diagram of a film reader according to an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The flow diagrams depicted in the figures are merely illustrative and not necessarily all of the elements and operations/steps are included or performed in the order described. For example, some operations/steps may be further divided, combined, or partially combined, so that the order of actual execution may be changed according to actual situations.
It is to be understood that the terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
The embodiment of the invention provides a blood smear scanning method, a film reader and a storage medium. The blood smear scanning method can be applied to a film reader to improve the scanning accuracy of blood smears and ensure the accuracy of inspection staff in film reading of scanned images.
Some embodiments of the invention are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.
Referring to fig. 1, fig. 1 is a flow chart of a blood smear scanning method according to an embodiment of the invention.
As shown in fig. 1, the blood smear scanning method includes steps S101 to S104.
And step S101, controlling a microscope device to scan the blood smear based on the first magnification, and stitching a plurality of first images obtained by scanning to obtain a first stitched image.
In the embodiment of the invention, the microscope device and the blood smear can relatively move, so that the microscope device can scan the blood smear. For example, the slide reader includes a microscope device, a loading platform for placing a blood smear, and a motion mechanism for driving the loading platform or the microscope device to move so that the microscope device and the blood smear can move relative to each other.
In an embodiment, in the process of scanning the blood smear based on the first magnification, the control microscope device controls the image overlapping rate between any two adjacent first images to be in a first overlapping rate range, wherein the image overlapping rate is an area proportion of a first overlapping region between two adjacent first images to any one of two adjacent second images. Wherein the first overlap ratio is in the range of 10% to 40% or 15% to 45%. Because the microscope device scans the blood smear based on the first magnification, the image overlapping rate between adjacent images is in the first overlapping rate range, so that cell omission caused by gaps in blood smear scanning can be avoided, and the accuracy of blood smear scanning is improved.
For example, as shown in fig. 2, the first image 21 is adjacent to the first image 22 in the first direction, the image overlapping ratio between the first image 21 and the first image 22 is in the first overlapping ratio range, that is, the ratio of the first overlapping area 24 between the first image 21 and the first image 22 to the area of the first image 21 or the second image 22 is in the first overlapping ratio range, the first image 21 is adjacent to the first image 23 in the second direction, the image overlapping ratio between the first image 21 and the first image 23 is in the first overlapping ratio range, that is, the ratio of the area of the first overlapping area 25 between the first image 21 and the first image 23 to the area of the first image 21 or the second image 23 is in the first overlapping ratio range.
In an embodiment, the method for controlling the microscope device to scan the blood smear based on the first magnification and stitch the plurality of first images obtained by scanning may include: controlling the microscope device to scan the blood smear row by row or column by column based on the first magnification; in the scanning process, splicing a plurality of first images obtained by scanning until the microscope device scans the blood smear to obtain a first spliced image; or after the microscope device scans the blood smear, stitching all the first images obtained by scanning to obtain a first stitched image. By splicing scanned images in the process of scanning the blood smear row by row or column by column, the time required by the whole blood smear scanning flow can be reduced, and the scanning efficiency of the blood smear is improved without waiting for outputting all the images and then splicing.
In an embodiment, in the scanning process, the stitching the plurality of first images obtained by scanning until the microscope device scans the blood smear may include: in the scanning process, a plurality of first images of the same row or the same column which are obtained through scanning are spliced to obtain a plurality of spliced sub-images, and the spliced sub-images are spliced until the blood smear is scanned by the microscope device to obtain a first spliced image. The image overlapping rate between adjacent spliced sub-images in the plurality of spliced sub-images is in a first overlapping rate range.
For example, let the blood smear include M lines, M is an integer greater than or equal to 10, the microscope device scans the blood smear line by line in the order of 1 st line to M-th line, and in the scanning process, after scanning the 1 st line, the first images obtained by scanning the 1 st line are stitched to obtain a 1 st line stitched sub-image, for example, as shown in fig. 3, the 1 st line is scanned to obtain the first image 31 and the first image 32, and the first image 31 and the first image 32 are stitched by having the first overlapping region 33 between the first image 31 and the first image 32, so that the 1 st line stitched sub-image 34 can be obtained.
After the 2 nd row is scanned, a plurality of first images obtained by scanning the 2 nd row are spliced to obtain a 2 nd row spliced sub-image, and the 1 st row spliced sub-image and the 2 nd row spliced sub-image are spliced to obtain 1 st to 2 nd row spliced sub-images; after the 3 rd line is scanned, a plurality of first images obtained by scanning the 3 rd line are spliced to obtain a 3 rd line spliced sub-image, the 3 rd line spliced sub-image and the 1 st line to 2 nd line spliced sub-image are spliced to obtain a 1 st line to 3 rd line spliced sub-image, the plurality of first images obtained by scanning each line and the spliced sub-image are spliced continuously according to the mode, and finally the plurality of first images obtained by scanning the M th line are spliced to obtain an M th line spliced sub-image, and the M th line spliced sub-image and the 1 st line to M-1 st line spliced sub-image are spliced to obtain a first spliced image. Therefore, the spliced image can be output in a short time after the blood smear is scanned, the time required by the whole blood smear scanning flow is reduced, and the scanning efficiency of the blood smear is improved.
In one embodiment, as shown in fig. 4, step S101 includes: substep S1011 to substep S1012.
Substep S1011, acquiring first position information of a body tail boundary region of the blood smear and second position information of a tail region and/or a coastline region of the blood smear.
In an embodiment of the invention, the blood smear comprises a body tail interface region, a tail region and a coastline region (edge region). For example, as shown in fig. 2, the blood smear 10 includes a head portion a, a body portion B, and a tail portion C, and the blood smear 10 includes a body-tail interface region 11, a tail portion region 12, and a coastline region 13. It will be appreciated that the head a, body B and tail C and the body tail interface region 11, tail region 12 and coastline region 13 of the blood smear in fig. 2 are merely exemplary and do not constitute a specific limitation of the head a, body B and tail C and the body tail interface region 11, tail region 12 and coastline region 13 of the blood smear.
In an embodiment, the method for obtaining the first position information of the body tail boundary region of the blood smear and the second position information of the tail region and/or the coastline region of the blood smear may be: controlling the microscope device to scan the blood smear based on the third magnification to obtain a plurality of second images, and splicing the plurality of second images to obtain a second spliced image, wherein the first magnification is larger than or equal to the third magnification; performing red blood cell overlapping rate identification on the second spliced image by running a preset identification model to obtain second red blood cell overlapping rate information of the second spliced image; according to the second red blood cell overlapping rate information, determining a region with the red blood cell overlapping rate in a first range in the second spliced image as a first imaging region corresponding to the tail boundary region; according to the second red blood cell overlapping rate information, determining a region with the red blood cell overlapping rate in a second range in the second spliced image as a second imaging region corresponding to the tail region and/or the coastline region; determining first position information of a tail boundary area according to the position of the first imaging area in the second spliced image; and determining second position information of the tail area and/or the coastline area according to the position of the second imaging area in the second spliced image. Through scanning and splicing the blood smear, based on the overlapping rate of red blood cells in each region in the spliced image, the imaging regions of the body tail junction region, the tail region and the coastline region of the blood smear can be accurately identified, and the position information of the body tail junction region, the tail region and the coastline region can be accurately calculated through the imaging regions, so that the blood smear can be accurately scanned according to the position information, and the identification accuracy of the target region is greatly improved.
The preset recognition model is obtained by training the neural network model based on a plurality of training sample images marked with the overlapping ratio of the red blood cells and a plurality of test sample images marked with the overlapping ratio of the red blood cells. The minimum value in the first range is greater than the maximum value in the second range, and in some embodiments, the first range and the second range may be the same, and the first range and the second range may be set based on actual situations, which is not specifically limited in the embodiment of the present invention. For example, the first range is 40% to 50%, and the second range is 1% to 10%.
In an embodiment, according to the position of the first imaging region in the second stitched image, the method for determining the first position information of the tail boundary region may be: and acquiring an image coordinate range of the first imaging region, and determining a mechanical coordinate range corresponding to the body tail boundary region according to a conversion relation between a pre-stored image coordinate system and a mechanical coordinate system and the image coordinate range of the first imaging region so as to obtain first position information of the body tail boundary region. The mechanical coordinate system is a rectangular coordinate system established by taking a reference point of the object carrying platform as an origin, the reference point of the object carrying platform is a position point of an optical coupler of the motion mechanism, the position point of the optical coupler is a return-to-zero position of a motor for driving the motion mechanism to move, and the conversion relationship between the image coordinate system and the mechanical coordinate system is calibrated in advance.
In an embodiment, the determining the second position information of the tail region and/or the coastline region according to the position of the second imaging region in the second stitched image may include: and acquiring an image coordinate range of the second imaging region, and determining a mechanical coordinate range corresponding to the tail region and/or the coastline region according to a conversion relation between a pre-stored image coordinate system and a mechanical coordinate system and the image coordinate range of the second imaging region so as to obtain second position information of the tail region and/or the coastline region.
In one embodiment, the manner in which the microscope device is controlled to scan the blood smear based on the third magnification may be: the control microscope device scans the blood smear in a smear direction or a direction opposite to the smear direction based on the third magnification. The direction of the pushing sheet of the blood smear is the direction that the head of the blood smear points to the tail, and the direction opposite to the direction of the pushing sheet is the direction that the tail of the blood smear points to the head. For example, as shown in fig. 5, the direction of the slide of the blood smear 10 is the direction in which the head portion a is directed toward the body tail portion C, and the direction opposite to the direction of the slide is the direction in which the tail portion C of the blood smear 10 is directed toward the head portion a. By scanning the blood smear in the direction of the push plate of the blood smear or in the direction opposite to the push plate direction, the scanning efficiency of the blood smear can be improved.
In an embodiment, the manner of controlling the microscope device to scan the blood smear in the direction of the smear or the direction opposite to the direction of the smear based on the third magnification may be: the control microscope device scans the middle region of the blood smear in the direction of the smear or in the direction opposite to the direction of the smear based on the third magnification. For example, as shown in fig. 6, the middle region of the blood smear is the region framed by the rectangular frame 14 in the blood smear 10. Because the middle area of the blood smear can comparatively complete characterization blood smear on the condition of body tail juncture area, afterbody region and coastline area, through carrying out the scanning to the middle area of blood smear along the shallow direction of blood smear or with the opposite direction of shallow direction like this, can further improve the scanning efficiency of blood smear under the circumstances of guaranteeing the scanning accuracy.
In an embodiment, the method for controlling the microscope device to scan the blood smear based on the third magnification to obtain a plurality of second images, and stitching the plurality of second images to obtain a second stitched image may be: controlling the microscope device to scan the blood smear row by row or column by column based on the third magnification; in the scanning process, splicing a plurality of second images obtained by scanning until the microscope device scans the blood smear to obtain a second spliced image; or after the microscope device scans the blood smear, stitching all second images obtained by scanning to obtain second stitched images.
For example, setting the whole region of the blood smear to include N columns, where N is an integer greater than or equal to 10, scanning the whole region of the blood smear column by the microscope device in the order of 1 st column to M-th column, and in the scanning process, stitching the plurality of second images obtained by scanning the 1 st column after scanning the 1 st column to obtain a 1 st column stitched sub-image, stitching the plurality of second images obtained by scanning the 2 nd column after scanning the 2 nd column to obtain a 2 nd column stitched sub-image, and stitching the 1 st column stitched sub-image and the 2 nd column stitched sub-image to obtain 1 st column to 2 nd column stitched sub-images; after the 3 rd column is scanned, a plurality of second images obtained by scanning the 3 rd column are spliced to obtain a 3 rd column spliced sub-image, the 3 rd column spliced sub-image and the 1 st column to 2 nd column spliced sub-image are spliced to obtain a 1 st column to 3 rd column spliced sub-image, the plurality of second images obtained by scanning each column and the spliced sub-image are spliced continuously according to the mode, and finally a plurality of second images obtained by scanning the M th column are spliced to obtain an M th column spliced sub-image, and the M th column spliced sub-image and the 1 st column to M-1 st column spliced sub-image are spliced to obtain a first spliced image. This allows the stitched image to be output in a short time that the entire region of the blood smear is scanned.
In an embodiment, in the process of scanning the blood smear based on the third magnification, the microscope device is controlled such that the image overlapping rate between any two adjacent second images is in a second overlapping rate range, and a maximum value in the first overlapping rate range is at least greater than a maximum value in the second overlapping rate range. The first overlap rate range and the second overlap rate range may be set based on actual situations, which is not specifically limited in the embodiment of the present invention. For example, the first overlap ratio ranges from 10% to 40% or 15% to 45%, and the second overlap ratio ranges from 5% to 10%, 10% to 20% or 15% to 30%. Because the microscope device scans the blood smear based on the third magnification, the image overlapping rate between adjacent images is in the second overlapping rate range, so that cell omission caused by gaps in the blood smear scanning process can be avoided, and the spliced images can describe the blood smear more comprehensively.
And step S1012, controlling the microscope device to scan the blood smear based on the first magnification according to the first position information and the second position information, and stitching a plurality of first images obtained by scanning to obtain a first stitched image.
In the embodiment of the invention, the first position information of the body tail junction area of the blood smear and the second position information of the tail area and/or the coastline area of the blood smear are firstly obtained, and then the microscope device is controlled to scan and splice the blood smear based on the first position information and the second position information, namely the body tail junction area, the tail area and the coastline area in the blood smear are scanned and spliced, so that the scanning efficiency of the blood smear can be further improved.
In an embodiment, according to the first position information and the second position information, the manner of controlling the microscope device to scan the blood smear based on the first magnification may be: determining a first motion path of the microscope device or the carrying platform according to the first position information and the second position information; according to the first motion path, the motion mechanism is controlled to move so as to drive the carrying platform or the microscope device to move along the first motion path, so that the microscope device can scan the tail boundary area, the tail area and the coastline area in the blood smear on the carrying platform.
Step S102, determining a first image area and a second image area in the first spliced image.
In the embodiment of the invention, the first image area is an imaging area corresponding to a body tail boundary area of the blood smear, and the second image area comprises an imaging area corresponding to a tail area of the blood smear and/or an imaging area corresponding to a coastline area. For example, as shown in fig. 5, the first image area is an imaging area corresponding to the body-tail boundary area 11, and the second image area includes an imaging area corresponding to the tail area 12 and/or an imaging area corresponding to the coastline area 13.
In an embodiment, the manner of determining the first image area and the second image area in the first stitched image may be: determining an image area corresponding to the first position information in the first spliced image as a first image area; and determining an image area corresponding to the second position information in the first spliced image as a second image area. The first position information is the position information of a body tail boundary area of the blood smear, and the second position information is the position information of a tail area and/or a coastline area of the blood smear.
In an embodiment, the manner of determining the first image area and the second image area in the first stitched image may be: operating a preset recognition model to recognize the overlapping rate of the red blood cells of the first spliced image, so as to obtain first overlapping rate information of the red blood cells of the first spliced image; according to the first red blood cell overlapping rate information, determining the areas with the red blood cell overlapping rate in the first spliced image in a preset first range as first image areas; and determining the area with the red blood cell overlapping rate in the first spliced image in a preset second range as a second image area according to the first red blood cell overlapping rate information. Wherein the minimum value in the first range is greater than the maximum value in the second range. The first red blood cell overlapping rate information comprises red blood cell overlapping rates of all areas in the first spliced image. Because the red blood cell overlapping rate in the head, the body and the tail in the blood smear is different, the imaging region corresponding to the body tail junction region, the imaging region corresponding to the tail region and the imaging region corresponding to the coastline region in the first spliced image can be accurately determined through the red blood cell overlapping rate of each region in the first spliced image.
Step S103, identifying and marking the cells of the first type in the first image area and identifying and marking the cells of the second type in the second image area to obtain a target image.
In an embodiment of the present invention, the cells of the first type may be the same or different from the cells of the second type, the cells of the first type may be nucleated cells, the cells of the second type may be non-nucleated cells, or the cells of the first type may comprise white blood cells, and the cells of the second type may comprise platelets. For example, neutrophils, eosinophils, basophils, monocytes, lymphocytes, nucleated erythrocytes, and smear cells in the first image region are identified and labeled, while platelets in the second image region are identified and labeled, resulting in a target image. The method can better identify nucleated cells such as white blood cells in the region with higher erythrocyte overlapping rate, and can better identify non-nucleated cells such as platelets in the region with lower erythrocyte overlapping rate, so that the identification accuracy of the cells can be improved.
In one embodiment, a first target recognition model is used to recognize and label cells of a first type in a first image region, and a second target recognition model is used to recognize and label cells of a second type in a second image region, so as to obtain a target image. The first target recognition model is obtained by training a neural network model based on a plurality of labeled first type of cell images, the second target recognition model is obtained by training a neural network model based on a plurality of labeled second type of cell images, and the neural network model can comprise a convolutional neural network (Convolutional Neural Networks, CNN) model, a deep neural network (Deep Neural Networks, DNN) model, a cyclic neural network (Recurrent Neural Network, RNN) model, a neural network (Factorisation Machine supported Neural Network, FNN) model supporting a decomposition machine, a neural factor decomposition machine (Neural Factorization Machine, NFM) model, and an attention factor decomposition machine (Attentional Factorization Machines, AFM) model.
Step S104, controlling the microscope device to scan the blood smear based on the second magnification according to the target image, so as to obtain a plurality of cell images.
In the embodiment of the invention, the second magnification is larger than the first magnification, and the first magnification is larger than or equal to the third magnification. The first magnification, the second magnification, and the third magnification may be set based on actual situations, which is not specifically limited in the embodiment of the present invention. For example, the first magnification and the third magnification are each 200 times (10-time eyepiece and 20-time objective lens), and the second magnification is 1000 times (10-time eyepiece and 100-time objective lens). For another example, the first magnification is 400 times, the third magnification is 200 times, and the second magnification is 1000 times.
In one embodiment, the microscope device comprises a first objective lens and a second objective lens, the first objective lens being a 20-fold objective lens or a 40-fold objective lens, the second objective lens being a 100-fold objective lens. Wherein, control the microscope device to scan the blood smear based on the first magnification, and splice a plurality of first images obtained by scanning, the obtaining a first stitched image may include: and controlling the microscope device to scan the blood smear by using the first objective lens, and splicing a plurality of first images obtained by scanning to obtain a first spliced image. Controlling the microscope device to scan the blood smear based on the second magnification according to the target image may include: and according to the target image, controlling the microscope device to scan the blood smear by using the second objective lens to obtain a plurality of cell images. Controlling the microscope device to scan the blood smear based on the third magnification to obtain a plurality of second images, and stitching the plurality of second images, the obtaining a second stitched image may include: and controlling the microscope device to scan the blood smear by using the first objective lens to obtain a plurality of second images, and stitching the plurality of second images to obtain a second stitched image.
In one embodiment, the microscope device includes a first objective lens, a second objective lens, and a third objective lens, the first objective lens is a 40-fold objective lens, the second objective lens is a 100-fold objective lens, and the third objective lens is a 20-fold objective lens. Wherein, control the microscope device to scan the blood smear based on the first magnification, and splice a plurality of first images obtained by scanning, the obtaining a first stitched image may include: and controlling the microscope device to scan the blood smear by using the first objective lens, and splicing a plurality of first images obtained by scanning to obtain a first spliced image. Controlling the microscope device to scan the blood smear based on the second magnification according to the target image may include: and according to the target image, controlling the microscope device to scan the blood smear by using the second objective lens to obtain a plurality of cell images. Controlling the microscope device to scan the blood smear based on the third magnification to obtain a plurality of second images, and stitching the plurality of second images, the obtaining a second stitched image may include: and controlling the microscope device to scan the blood smear by using a third object lens to obtain a plurality of second images, and stitching the plurality of second images to obtain a second stitched image.
In one embodiment, as shown in FIG. 7, steps S1041 through S1042 are performed in step S104.
Substep S1041, acquiring position coordinates of the first type of cells and the second type of cells according to the target image.
In an embodiment of the present invention, the cells of the first type may be the same or different from the cells of the second type, the cells of the first type may be nucleated cells, the cells of the second type may be non-nucleated cells, or the cells of the first type may comprise white blood cells, and the cells of the second type may comprise platelets.
In one embodiment, mechanical coordinates corresponding to a reference point in a target image are acquired; determining a pixel distance of each target cell in the target image relative to a reference point, the target cells including cells of a first type and cells of a second type; determining the actual distance of each target cell on the blood smear relative to the reference point according to the conversion relation between the pre-stored image coordinate system and the mechanical coordinate system and the pixel distance of each target cell relative to the reference point; and determining the respective mechanical coordinates of each target cell on the blood smear according to the actual distance of each target cell on the blood smear relative to the reference point and the mechanical coordinates corresponding to the reference point, thereby obtaining the position coordinates of each cell of the first type and each cell of the second type on the blood smear. The conversion relationship between the image coordinate system and the mechanical coordinate system can be calibrated in advance, which is not particularly limited in the embodiment of the invention.
In an embodiment, acquiring the mechanical coordinates corresponding to the reference point in the target image may include: and determining the center point of the target image as a reference point, and acquiring mechanical coordinates corresponding to the reference point. Or determining a position point of the central point of the first image in the target image, determining the position point as a reference point, and acquiring mechanical coordinates corresponding to the reference point.
Step S1042, according to each position coordinate, sequentially controlling the microscope device to shoot the corresponding cells based on the second magnification, so as to obtain a plurality of cell images.
In the embodiment of the invention, when the microscope device shoots any cell based on the second magnification, the relative movement of the microscope device and the object carrying platform is controlled according to the position coordinate corresponding to the cell, so that the cell at the position coordinate is positioned in the visual field of the microscope device; controlling a microscope device to shoot cells at different depth-of-field distances within a preset depth-of-field distance range at a preset focal point position to obtain a plurality of candidate cell images; the definition of each candidate cell image in the plurality of candidate cell images is determined, and the candidate cell image corresponding to the highest definition is determined as the final cell image. The preset focal position and the preset depth-of-field distance range may be set based on practical situations, which is not specifically limited in the embodiment of the present invention.
In an embodiment, according to each position coordinate, the manner of sequentially controlling the microscope device to capture the corresponding cells based on the second magnification to obtain the plurality of cell images may be: planning a second motion path of the microscope device or the object carrying platform according to the position coordinates of the first type of cells and the second type of cells, wherein the second motion path comprises shooting points corresponding to each position coordinate; controlling the movement mechanism to move according to the second movement path so as to drive the microscope device or the carrying platform to move along the second movement path; when the microscope device or the object carrying platform is determined to be located at any shooting point in the second motion path, the microscope device is controlled to shoot the cells at the position of the shooting point based on the second magnification, and a cell image is obtained.
In one embodiment, after step S104, the method further includes: controlling the display device to display the target image; based on the position coordinates of the cells selected by the user in the target image, the display device is controlled to display the cell image corresponding to the position coordinates. For example, as shown in fig. 8, 28 cells are marked on the displayed target image in total, and each cell is marked with a number. When the user selects the cell number 10 in the target image, the position coordinates of the cell number 10 are acquired, and the display device is controlled to display the cell image corresponding to the position coordinates (the cell image corresponding to the cell number 10). After the target image is displayed, the user can select cells to be observed carefully from the target image, so that the film reader displays the cell image corresponding to the position coordinates of the cells selected by the user, and the user can observe conveniently.
In one embodiment, after step S104, the method further includes: the control display device displays the target image and the plurality of cell images. The marks on the cell image are the same as the marks of the corresponding cells in the target image, and the display device can be a display screen on the film reader or a display screen on a terminal device in communication connection with the film reader. For example, as shown in fig. 8, 28 cells are marked on the target image in total, and each cell is marked with a number. As shown in fig. 9, the display device displays 28 cell images, and the number of each cell image displayed is the same as the number of the corresponding cell within the target image in fig. 8. Through controlling the display device to display the target image and the plurality of cell images, the user can check the whole cell distribution condition of the blood smear through the target image, and can check single cells through the cell images, so that the convenience of film reading is improved, for example, when the recheck is triggered, a doctor can obtain the film reading result in morphology and the result of single cells of film reading, and the abnormal cause of missed detection can be obtained rapidly.
In one embodiment, after step S104, the method further includes: controlling the display device to display the target image and the plurality of cell images; acquiring the position coordinates of cells selected by a user in a target image; based on the position coordinates, the relative movement of the microscope device and the blood smear is controlled so that the cells corresponding to the position coordinates are within the field of view of the microscope device. When the problems of unclear and abnormal cell images are found, a user can select cells needing to be observed again in the target image, so that the film reader can acquire the position coordinates of the cells which the user wants to observe, and control the relative movement of the microscope device and the blood smear according to the position coordinates, so that the cells at the position coordinates are positioned in the field of view of the microscope device, and the user can conveniently conduct review observation.
Referring to fig. 10, fig. 10 is a schematic block diagram of a film reader according to an embodiment of the present invention.
As shown in fig. 10, the reader 200 includes a microscope device 201, a processor 202, and a memory 203, and the microscope device 201, the processor 202, and the memory 203 are connected by a bus 204, such as an I2C (Inter-integrated Circuit) bus.
In particular, the processor 202 is configured to provide computing and control capabilities to support the operation of the entire reader. The processor 202 may be a central processing unit (Central Processing Unit, CPU), and the processor 202 may also be other general purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), field-programmable gate arrays (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. Wherein the general purpose processor may be a microprocessor or the processor may be any conventional processor or the like.
Specifically, the Memory 203 may be a Flash chip, a Read-Only Memory (ROM) disk, an optical disk, a U-disk, a removable hard disk, or the like.
It will be appreciated by those skilled in the art that the structure shown in fig. 10 is merely a block diagram of a portion of the structure associated with an embodiment of the present invention and is not intended to limit the reader to which an embodiment of the present invention may be applied, and that a particular reader may include more or fewer components than shown, or may combine certain components, or may have a different arrangement of components.
The processor is used for running a computer program stored in the memory, and implementing any one of the blood smear scanning methods provided by the embodiment of the invention when the computer program is executed.
In an embodiment, the processor is configured to run a computer program stored in a memory and to implement the following steps when the computer program is executed:
controlling a microscope device to scan the blood smear based on a first magnification, and stitching a plurality of first images obtained by scanning to obtain a first stitched image;
determining a first image area and a second image area in the first spliced image, wherein the first image area is an imaging area corresponding to a body tail juncture area of the blood smear, and the second image area comprises an imaging area corresponding to a tail area of the blood smear and/or an imaging area corresponding to a coastline area;
Identifying and marking cells of a first type in the first image area and identifying and marking cells of a second type in the second image area to obtain a target image;
and controlling the microscope device to scan the blood smear based on the second magnification according to the target image to obtain a plurality of cell images.
In an embodiment, the processor, when implementing determining the first image region and the second image region in the first stitched image, is configured to implement:
operating a preset recognition model to recognize the overlapping rate of the red blood cells of the first spliced image, so as to obtain first overlapping rate information of the red blood cells of the first spliced image;
determining the region with the overlapping ratio of the red blood cells in the first spliced image in a preset first range as the first image region according to the first red blood cell overlapping ratio information;
determining the region with the overlapping ratio of the red blood cells in the first spliced image in a preset second range as the second image region according to the first red blood cell overlapping ratio information;
wherein the minimum value in the first range is greater than the maximum value in the second range.
In an embodiment, the processor is configured to, when implementing control of the microscope device to scan the blood smear based on the first magnification, and stitch a plurality of first images obtained by the scanning to obtain a first stitched image, implement:
controlling a microscope device to scan the blood smear row by row or column by column based on a first magnification;
in the scanning process, splicing the plurality of first images obtained by scanning until the microscope device finishes scanning the blood smear so as to obtain a first spliced image;
or after the microscope device scans the blood smear, stitching all the first images obtained by scanning to obtain a first stitched image.
In an embodiment, the processor is configured to, when implementing control of the microscope device to scan the blood smear based on the first magnification, and stitch a plurality of first images obtained by the scanning to obtain a first stitched image, implement:
acquiring first position information of a body tail boundary region of the blood smear and second position information of a tail region and/or a coastline region of the blood smear;
and controlling a microscope device to scan the blood smear based on the first magnification according to the first position information and the second position information, and splicing a plurality of first images obtained by scanning to obtain the first spliced image.
In an embodiment, the processor is configured to, when implementing obtaining the first position information of the body tail boundary region of the blood smear and the second position information of the tail region and/or coastline region of the blood smear, implement:
controlling a microscope device to scan the blood smear based on a third magnification to obtain a plurality of second images, and splicing the plurality of second images to obtain a second spliced image, wherein the first magnification is larger than or equal to the third magnification;
operating a preset recognition model to recognize the overlapping rate of the red blood cells of the second spliced image, so as to obtain second overlapping rate information of the red blood cells of the second spliced image;
determining a region with the red blood cell overlapping rate in a first range in the second spliced image as a first imaging region corresponding to the tail boundary region according to the second red blood cell overlapping rate information;
determining a region with the red blood cell overlapping rate in a second range in the second spliced image as a second imaging region corresponding to the tail region and/or the coastline region according to the second red blood cell overlapping rate information;
determining first position information of the body tail junction area according to the position of the first imaging area in the second spliced image;
And determining second position information of the tail area and/or the coastline area according to the position of the second imaging area in the second spliced image.
In an embodiment, the processor, when implementing determining the first image region and the second image region in the first stitched image, is configured to implement:
determining an image area corresponding to the first position information in the first spliced image as the first image area;
and determining an image area corresponding to the second position information in the first spliced image as the second image area.
In an embodiment, the processor, when implementing controlling the microscope device to scan the blood smear based on the third magnification, is configured to implement:
the microscope device is controlled to scan the blood smear in a direction of the smear or a direction opposite to the direction of the smear based on the third magnification.
In an embodiment, the processor is configured to, when implementing controlling the microscope device to scan the blood smear in a smear direction or a direction opposite to the smear direction based on the third magnification, implement:
the microscope device is controlled to scan the middle region of the blood smear in a direction of the smear or a direction opposite to the direction of the smear based on the third magnification.
In an embodiment, in the process of scanning the blood smear by the control microscope device based on the first magnification, the image overlapping rate between any two adjacent first images is in a first overlapping rate range; in the process of scanning the blood smear based on the third magnification, the control microscope device controls the image overlapping rate between any two adjacent second images to be in a second overlapping rate range; the maximum value in the first overlap ratio range is at least greater than the maximum value in the second overlap ratio range.
In one embodiment, the processor is configured to, when implementing scanning the blood smear based on the second magnification according to the target image, control the microscope device to implement:
acquiring the position coordinates of the first type of cells and the second type of cells according to the target image;
and according to each position coordinate, sequentially controlling the microscope device to shoot the corresponding cells based on the second magnification, and obtaining a plurality of cell images.
In one embodiment, the cells of the first type comprise white blood cells and the cells of the second type comprise platelets.
In one embodiment, the first type of cell is the same as or different from the second type of cell.
In an embodiment, the processor is further configured to, after implementing scanning the blood smear based on the second magnification according to the target image, implement:
controlling a display device to display the target image;
and controlling a display device to display the cell image corresponding to the position coordinates based on the position coordinates of the cell selected by the user in the target image.
In an embodiment, the processor is further configured to, after implementing scanning the blood smear based on the second magnification according to the target image, implement:
controlling a display device to display the target image and a plurality of the cell images;
acquiring the position coordinates of cells selected by a user in the target image;
and controlling the relative movement of the microscope device and the blood smear according to the position coordinates so that cells corresponding to the position coordinates are in the visual field of the microscope device.
It should be noted that, for convenience and brevity of description, the specific working process of the film reader described above may refer to the corresponding process in the foregoing blood smear scanning method embodiment, and will not be described herein.
The embodiment of the invention also provides a storage medium for computer readable storage, wherein the storage medium stores one or more programs, and the one or more programs can be executed by one or more processors to realize any blood smear scanning method provided by the embodiment of the invention.
The storage medium may be an internal storage unit of the film reader according to the foregoing embodiment, for example, a hard disk or a memory of the film reader. The storage medium may also be an external storage device of the reader, such as a plug-in hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card) or the like, which are provided on the reader.
Those of ordinary skill in the art will appreciate that all or some of the steps, systems, functional modules/units in the apparatus, and methods disclosed above may be implemented as software, firmware, hardware, and suitable combinations thereof. In a hardware embodiment, the division between the functional modules/units mentioned in the above description does not necessarily correspond to the division of physical components; for example, one physical component may have multiple functions, or one function or step may be performed cooperatively by several physical components. Some or all of the physical components may be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application specific integrated circuit. Such software may be distributed on computer readable media, which may include computer storage media (or non-transitory media) and communication media (or transitory media). The term computer storage media includes both volatile and nonvolatile, removable and non-removable media implemented in any method or technology for storage of information such as computer readable instructions, data structures, program modules or other data, as known to those skilled in the art. Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technology, CD-ROM, digital Versatile Disks (DVD) or other optical disk storage, magnetic cassettes, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other medium which can be used to store the desired information and which can be accessed by a computer. Furthermore, as is well known to those of ordinary skill in the art, communication media typically embodies computer readable instructions, data structures, program modules or other data in a modulated data signal such as a carrier wave or other transport mechanism and includes any information delivery media.
It should be understood that the term "and/or" as used in the present specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The foregoing embodiment numbers of the present invention are merely for the purpose of description, and do not represent the advantages or disadvantages of the embodiments. While the invention has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Claims (16)
1. A blood smear scanning method comprising:
controlling a microscope device to scan the blood smear based on a first magnification, and stitching a plurality of first images obtained by scanning to obtain a first stitched image;
determining a first image area and a second image area in the first spliced image, wherein the first image area is an imaging area corresponding to a body tail juncture area of the blood smear, and the second image area comprises an imaging area corresponding to a tail area of the blood smear and/or an imaging area corresponding to a coastline area;
identifying and marking cells of a first type in the first image area and identifying and marking cells of a second type in the second image area to obtain a target image;
and controlling the microscope device to scan the blood smear based on the second magnification according to the target image to obtain a plurality of cell images.
2. The blood smear scan method of claim 1, wherein the determining a first image region and a second image region in the first stitched image comprises:
operating a preset recognition model to recognize the overlapping rate of the red blood cells of the first spliced image, so as to obtain first overlapping rate information of the red blood cells of the first spliced image;
Determining the region with the overlapping ratio of the red blood cells in the first spliced image in a preset first range as the first image region according to the first red blood cell overlapping ratio information;
determining the region with the overlapping ratio of the red blood cells in the first spliced image in a preset second range as the second image region according to the first red blood cell overlapping ratio information;
wherein the minimum value in the first range is greater than the maximum value in the second range.
3. The method of claim 1, wherein the controlling the microscope device to scan the blood smear based on the first magnification and stitch the plurality of first images obtained by the scanning to obtain a first stitched image comprises:
controlling a microscope device to scan the blood smear row by row or column by column based on a first magnification;
in the scanning process, splicing the plurality of first images obtained by scanning until the microscope device finishes scanning the blood smear so as to obtain a first spliced image;
or after the microscope device scans the blood smear, stitching all the first images obtained by scanning to obtain a first stitched image.
4. The method of claim 1, wherein the controlling the microscope device to scan the blood smear based on the first magnification and stitch the plurality of first images obtained by the scanning to obtain a first stitched image comprises:
acquiring first position information of a body tail boundary region of the blood smear and second position information of a tail region and/or a coastline region of the blood smear;
and controlling a microscope device to scan the blood smear based on the first magnification according to the first position information and the second position information, and splicing a plurality of first images obtained by scanning to obtain the first spliced image.
5. The method of claim 4, wherein the acquiring the first position information of the body-tail junction region of the blood smear and the second position information of the tail region and/or coastline region of the blood smear comprises:
controlling a microscope device to scan the blood smear based on a third magnification to obtain a plurality of second images, and splicing the plurality of second images to obtain a second spliced image, wherein the first magnification is larger than or equal to the third magnification;
Operating a preset recognition model to recognize the overlapping rate of the red blood cells of the second spliced image, so as to obtain second overlapping rate information of the red blood cells of the second spliced image;
determining a region with the red blood cell overlapping rate in a first range in the second spliced image as a first imaging region corresponding to the tail boundary region according to the second red blood cell overlapping rate information;
determining a region with the red blood cell overlapping rate in a second range in the second spliced image as a second imaging region corresponding to the tail region and/or the coastline region according to the second red blood cell overlapping rate information;
determining first position information of the body tail junction area according to the position of the first imaging area in the second spliced image;
and determining second position information of the tail area and/or the coastline area according to the position of the second imaging area in the second spliced image.
6. The blood smear scan method of claim 5, wherein said determining a first image region and a second image region in the first stitched image comprises:
determining an image area corresponding to the first position information in the first spliced image as the first image area;
And determining an image area corresponding to the second position information in the first spliced image as the second image area.
7. The blood smear scanning method according to claim 5, wherein the controlling the microscope device to scan the blood smear based on the third magnification comprises:
the microscope device is controlled to scan the blood smear in a direction of the smear or a direction opposite to the direction of the smear based on the third magnification.
8. The blood smear scanning method according to claim 5, wherein the controlling the microscope device to scan the blood smear in a smear direction of the blood smear or a direction opposite to the smear direction based on the third magnification includes:
the microscope device is controlled to scan the middle region of the blood smear in a direction of the smear or a direction opposite to the direction of the smear based on the third magnification.
9. The method according to claim 5, wherein the control microscope device scans the blood smear based on a first magnification, and wherein an image overlapping ratio between any adjacent two of the first images is in a first overlapping ratio range;
In the process of scanning the blood smear based on the third magnification, the control microscope device controls the image overlapping rate between any two adjacent second images to be in a second overlapping rate range;
the maximum value in the first overlap ratio range is at least greater than the maximum value in the second overlap ratio range.
10. The method of any one of claims 1-8, wherein controlling the microscope device to scan the blood smear based on the second magnification to obtain a plurality of cell images according to the target image comprises:
acquiring the position coordinates of the first type of cells and the second type of cells according to the target image;
and according to each position coordinate, sequentially controlling the microscope device to shoot the corresponding cells based on the second magnification, and obtaining a plurality of cell images.
11. The blood smear scan method of claim 10, wherein the first type of cells comprises white blood cells and the second type of cells comprises platelets.
12. The blood smear scan method of claim 10, wherein the cells of the first type are the same or different from the cells of the second type.
13. The method according to any one of claims 1 to 8, wherein the controlling the microscope device to scan the blood smear based on the second magnification according to the target image, after obtaining a plurality of cell images, further comprises:
controlling a display device to display the target image;
and controlling a display device to display the cell image corresponding to the position coordinates based on the position coordinates of the cell selected by the user in the target image.
14. The method according to any one of claims 1 to 8, wherein the controlling the microscope device to scan the blood smear based on the second magnification according to the target image, after obtaining a plurality of cell images, further comprises:
controlling a display device to display the target image and a plurality of the cell images;
acquiring the position coordinates of cells selected by a user in the target image;
and controlling the relative movement of the microscope device and the blood smear according to the position coordinates so that cells corresponding to the position coordinates are in the visual field of the microscope device.
15. A film reader comprising a microscope device, a processor, a memory, a computer program stored on the memory and executable by the processor, and a data bus for enabling a connection communication between the processor and the memory, wherein the computer program, when executed by the processor, implements a blood smear scanning method according to any of claims 1 to 14.
16. A storage medium for computer readable storage, wherein the storage medium stores one or more programs executable by one or more processors to implement the blood smear scanning method of any one of claims 1 to 14.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211618371.7A CN116046645A (en) | 2022-12-15 | 2022-12-15 | Blood smear scanning method, film reader and storage medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211618371.7A CN116046645A (en) | 2022-12-15 | 2022-12-15 | Blood smear scanning method, film reader and storage medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116046645A true CN116046645A (en) | 2023-05-02 |
Family
ID=86132189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211618371.7A Pending CN116046645A (en) | 2022-12-15 | 2022-12-15 | Blood smear scanning method, film reader and storage medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116046645A (en) |
-
2022
- 2022-12-15 CN CN202211618371.7A patent/CN116046645A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11721018B2 (en) | System and method for calculating focus variation for a digital microscope | |
| US8098956B2 (en) | Digital microscope slide scanning system and methods | |
| US7873193B2 (en) | Serial section analysis for computer-controlled microscopic imaging | |
| CN109886928B (en) | Target cell marking method, device, storage medium and terminal equipment | |
| US11434082B2 (en) | Stuck slide determination system | |
| US9338408B2 (en) | Image obtaining apparatus, image obtaining method, and image obtaining program | |
| CN112714887B (en) | Microscope system, projection unit, and image projection method | |
| JP2009528580A (en) | Method for digitally photographing slides and automatic digital image recording system therefor | |
| CN112464802B (en) | Automatic identification method and device for slide sample information and computer equipment | |
| US20240205546A1 (en) | Impulse rescan system | |
| JP2009122115A (en) | Cell image analyzer | |
| US20110115896A1 (en) | High-speed and large-scale microscope imaging | |
| CN105122037A (en) | Image processing system, image processing device, program, storage medium, and image processing method | |
| JP2005227097A (en) | Cell image analyzer | |
| CN116046645A (en) | Blood smear scanning method, film reader and storage medium | |
| US20200074628A1 (en) | Image processing apparatus, imaging system, image processing method and computer readable recoding medium | |
| CN112322713B (en) | Imaging method, device and system and storage medium | |
| US8482820B2 (en) | Image-capturing system | |
| CN108647549B (en) | Bar code image processing method, device and system | |
| CN112132139A (en) | Character recognition method and device | |
| US20200349676A1 (en) | Image processing device, system, and method for improving signal-to-noise of microscopy images | |
| CN118158524A (en) | Imaging method, device and system | |
| CN118276298A (en) | Slide scanning method, slide reader and storage medium | |
| KR102085755B1 (en) | Apparatus for photographing synapse image and operating mehtod thereof | |
| Wang et al. | Design, Integration, and Verification of a Low-Cost Nailfold Capillaroscopy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |