CN116041408A - Preparation method and application of flavonol compound dysosma versipellis A-F - Google Patents
Preparation method and application of flavonol compound dysosma versipellis A-F Download PDFInfo
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- CN116041408A CN116041408A CN202310045919.1A CN202310045919A CN116041408A CN 116041408 A CN116041408 A CN 116041408A CN 202310045919 A CN202310045919 A CN 202310045919A CN 116041408 A CN116041408 A CN 116041408A
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- 241000332747 Dysosma versipellis Species 0.000 title claims abstract description 74
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- -1 flavonol compound Chemical class 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 228
- 239000012046 mixed solvent Substances 0.000 claims description 109
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 90
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 88
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/44—Preservation of non-alcoholic beverages by adding preservatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3544—Organic compounds containing hetero rings
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- C07—ORGANIC CHEMISTRY
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- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/28—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
- C07D311/30—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/22—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
- C07D311/26—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
- C07D311/40—Separation, e.g. from natural material; Purification
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- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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Abstract
The invention relates to flavonol compounds, a preparation method and application thereof, which can effectively solve the problems of preparation of flavonol compounds and preparation of antioxidants, wherein the flavonol compounds are dysosma versipellis ketone A (dysosmaflavonoid A), dysosma versipellis ketone B (dysosmaflavonoid B), dysosma versipellis ketone C (dysosmaflavonoid C), dysosma versipellis ketone D (dysosmaflavonoid D), dysosma versipellis ketone E (dysosmaflavonoid E) and dysosma versipellis ketone F (dysosmaflavonoid F) extracted from dysosma versipellis.
Description
Technical Field
The invention relates to the field of medicines, in particular to a preparation method and application of flavonol compound dysosma versipellis A-F with antioxidant activity.
Background
Oxidative stress means that active oxygen free radicals generated in tissues or cells of a body exceed the original oxygen free radical utilization capacity or scavenging capacity, and redox balance is destroyed, so that excessive accumulated active oxygen free radicals and related metabolites thereof are generated in the body to cause certain damage to the body, and certain pathological conditions are presented. Oxidative stress is thought to be responsible for many degenerative diseases such as cancer, chronic inflammation, cardiovascular disease, neurological disease, diabetes, alzheimer's disease, parkinson's disease, and the like. Thus, inhibition of oxidative stress-like damage has become one of the important ways to treat diseases associated with oxidative stress. At the same time, in foods, oxidation of nutrients can cause degradation of the food and even disease of the body of the ingester. Thus, the search for safe antioxidants has been a hotspot technology for pharmaceutical and nutritional research.
Dysosma versipellis is the dried rhizome of Dysosma versipellis Dysosma versipellis of berberidaceae, and is mainly distributed in Zhejiang, guangxi, hubei, hunan, sichuan, guizhou, etc. Has effects of clearing heat and detoxicating, eliminating phlegm and resolving masses, and relieving pain and detumescence. Is used for treating traumatic injury, hemiplegia, joint ache, venomous snake bite, sore, carbuncle, toxic swelling, condyloma acuminatum, epidemic hemorrhagic fever, japanese encephalitis, lymphadenitis, parotitis, breast cancer, esophagus cancer and other inflammatory diseases. The dysosma versipellis contains a large amount of aryl naphthalene lactone type lignans and flavonoid compounds, and the flavonoid compounds from natural sources have pharmacological actions of anti-inflammatory, anti-cancer, anti-aging, heart protection, neuroprotection, immunoregulation, anti-diabetes, antibacterial, antiviral, antioxidation, antiparasitic and the like. The invention relates to a preparation method of flavonol compounds dysosma versipellis A-F and application of the flavonol compounds dysosma versipellis A-F in food and beverage in antioxidant biological activity, and has not been reported so far.
Disclosure of Invention
Aiming at the situation, the invention aims to overcome the defects of the prior art and provide a preparation method and application of the flavonol compound dysosma versipellis A-F, which can effectively solve the problems of preparation of flavonol compounds and preparation of antioxidants.
The technical scheme is that the flavonol compound is dysosma versipellis ketone A-F (dysosmaflavonoids A-F) extracted from dysosma versipellis, and the molecular structural formulas are respectively as follows:
the preparation method comprises the following steps:
20-40kg of dysosma versipellis medicinal material is taken as a raw material, 3-5 times of ethanol with the weight volume and the volume concentration of 95% is added each time, the ethanol is heated and refluxed for extraction for 3 times, the weight volume is calculated by kg of solid, the liquid is calculated by L, the extraction temperature is 92-95 ℃, the extraction time is 1-1.5 hours each time, and the ethanol with the volume concentration of 95% is recovered under reduced pressure to obtain an extract of 95% ethanol; adding 3-5 times of the raw materials into the residuesReflux-extracting with 50% ethanol for 1 time at 92-95deg.C for 1-1.5 hr under reduced pressure to obtain extract 50% ethanol extract; mixing 95% ethanol extract and 50% ethanol extract, adding anhydrous ethanol 2 times the weight of the extract for dissolution, adding diatomite 2 times the weight of the extract for adsorption, recovering solvent, sequentially adding dichloromethane, ethyl acetate and methanol 3 times the weight of the extract for elution, and recovering solvent to obtain dichloromethane elution part, ethyl acetate elution part and methanol elution part respectively; separating the methanol elution part by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient uses 30L-60L eluent with the flow rate of 50-70mL/min, each 5L-10L is a fraction, 54 fractions are collected, and each fraction is detected and analyzed by silica gel thin layer chromatography and GF is used 254 The thin layer plate is prepared by respectively taking petroleum ether-acetone with the volume ratio of 4:5 and methylene dichloride-methanol with the volume ratio of 5:2 as developing agents, taking sulfuric acid-ethanol solution with the volume ratio of 10:90 as a developing agent, heating for 3-5min at the temperature of 105 ℃, and respectively combining 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts according to the detection result of thin layer chromatography to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 by sephadex LH-20 gel column chromatography, eluting with 5.0L-10L of methanol at a flow rate of 5-10mL/min, collecting 50 fractions each 100-200 mL as one fraction, and respectively combining 1-25 fractions, 26-44 fractions and 45-50 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, a subfraction Fr.M4-2 and a subfraction Fr.M4-3; the subfraction Fr.M4-1 was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient is 2.5L-5L, each gradient is a subfraction, and subfractions Fr.M4- 1-1, subfraction Fr.M4-1-2, subfraction Fr.M4-1-3, subfraction Fr.M4-1-4, subfraction Fr.M4-1-5, subfraction Fr.M4-1-6; separating the subfractions Fr.M4-1-2 by sephadex LH-20 gel column chromatography, eluting with 500-1000mL of methanol, taking each 5-10 mL as a fraction, collecting 100 fractions, respectively combining the fractions 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 by silica gel thin layer chromatography detection analysis, and collecting the fractions to obtain subfractions Fr.M4-1-2-1, subfractions Fr.M4-1-2-2, subfractions Fr.M4-1-2-3, subfractions Fr.M4-1-2-4, subfractions Fr.M4-1-2-5 and subfractions Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 by preparative high performance liquid chromatography, eluting with se:Sup>A mixed solvent of methanol and water with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively for the chromatographic column YMC-Pack ODS-A, the flow rate of 3 m/min, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min respectively at flow rate of 3 m/min and YMC-Pack ODS-A column to obtain Dysosmse:Sup>A versipellis E (dysosmaflavonoid E);
Purifying the subfraction Fr.M4-1-2-3-4 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with flow rate of 3 m/min and retention time of 30.7min to obtain Dysosmse:Sup>A versipellis F (dysosmaflavonoid F);
separating the component Fr.M5 by sephadex LH-20 column chromatography, eluting with 2.5L-5L methanol at a flow rate of 5-10mL/min, collecting 25 fractions each 100mL-200mL as one fraction, and respectively combining 1-3, 4-5, 6-7, 8-10, 11-13, 14-17, 19-21, 22-25 to obtain subcomponents Fr.M5-1,Subfraction Fr.M5-2, subfraction Fr.M5-3, subfraction Fr.M5-4, subfraction Fr.M5-5, subfraction Fr.M5-6, subfraction Fr.M5-7, subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 are combined, separated by MCI column chromatography and separated by MeOH-H with volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Carrying out gradient elution on an O mixed solvent system, wherein each gradient elution solvent is 1.0L-2.0L, the flow rate is 2.5-5.0mL/min, each 250mL-500mL is a fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-15 fractions, 16-23 fractions and 17-28 fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 were separated by column chromatography on silica gel with CHCl in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 3 Gradient elution of a MeOH mixed solvent system, wherein each gradient elution solvent is 50-100 mL, the flow rate is 2.0-5.0mL/min, each 5mL-10mL is one fraction, 80 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain a subfraction Fr.M5-1-3-1, a subfraction Fr.M5-1-3-2, a subfraction Fr.M5-1-3-3, a subfraction Fr.M5-1-3-5, a subfraction Fr.M5-1-3-7, a subfraction Fr.M5-1-3-8, and a subfraction Fr.M5-1-3-9; purifying the subfraction Fr.M5-1-3-5 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain Dysosmse:Sup>A versipellis A (dysosmaflavonoid A);
separating the component Fr.M6 by sephadex LH-20 column chromatography, eluting with 15L-30L methanol at a flow rate of 5-10mL/min, collecting 30 fractions each 0.5L-1L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1 and Fr.M6-2; separating the subfraction Fr.M6-1 by ODS column chromatography to obtain the final product MeOH-H with product ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out on the O mixed solvent system, wherein each gradient elution solvent is 2.5L-5L, the flow rate is 3.0-6.0mL/min, each 0.5L-1L is one fraction, 30 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are respectively combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 125L-250L, the flow rate is 1.0-2.0mL/min, and each elution gradient is one flow part, so as to obtain subfractions Fr.M6-1-3-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating the subfractions Fr.M6-1-3-4 by sephadex LH-20 gel column chromatography, eluting with 125mL-250mL of methanol at a flow rate of 1-2mL/min, collecting 25 fractions each of which is 5mL-10mL, and respectively combining the fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively for the chromatographic column YMC-Pack ODS-A and flow rate of 3 m/min, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying the subfraction Fr.M6-1-3-4-2-1 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C (dysosmaflavonoid C);
Purifying the subfraction Fr.M6-1-3-4-2-2 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D (dysosmaflavonoid D);
purifying the subfraction Fr.M6-1-3-4-2-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 28.5min, and collecting the compound dysosmse:Sup>A versipellis B (dysosmaflavonoid B).
The novel flavonol compounds dysosma versipellis A-F with antioxidant activity, which are prepared by the invention, can be used for preparing antioxidants for foods or beverages, and has the advantages of abundant raw materials, easy operation of the preparation method and remarkable economic and social benefits.
Drawings
FIG. 1 is a diagram of the molecular structure of the present invention.
FIG. 2 shows dysosma versipellis A of the present invention 1 H NMR spectrum (500 MHz).
FIG. 3 shows dysosma versipellis A of the present invention 13 C NMR spectrum (125 MHz).
FIG. 4 is a 1H NMR spectrum (500 MHz) of dysosma versipellis B of the invention.
FIG. 5 shows dysosma versipellis B of the present invention 13 C NMR spectrum (125 MHz).
FIG. 6 shows dysosma versipellis C of the present invention 1 H NMR spectrum (500 MHz).
FIG. 7 shows dysosma versipellis C of the present invention 13 C NMR spectrum (125 MHz).
FIG. 8 shows dysosma versipellis D of the present invention 1 H NMR spectrum (500 MHz).
FIG. 9 shows dysosma versipellis D of the present invention 13 C NMR spectrum (125 MHz).
FIG. 10 shows dysosma versipellis E of the present invention 1 H NMR spectrum (500 MHz).
FIG. 11 shows dysosma versipellis E of the present invention 13 C NMR spectrum (125 MHz).
FIG. 12 shows dysosma versipellis F of the present invention 1 H NMR spectrum (500 MHz).
FIG. 13 shows dysosma versipellis F of the present invention 13 C NMR spectrum (125 MHz).
Detailed Description
The following describes specific embodiments of the present invention in detail with reference to examples.
The invention may be embodied by the following examples.
Example 1
The invention relates to a preparation method of flavonol compound dysosma versipellis ketone A-F, which takes 40kg of dysosma versipellis medicinal material as raw material, adds 3 times of ethanol with the weight volume and the volume concentration of 95% of the raw material each time, carries out heating reflux extraction for 3 times, the extraction temperature is 95 ℃, the extraction time is 1 hour each time, and decompresses and recovers 95% ethanol to obtain extract 95% ethanol extract; adding 3 times of ethanol with weight volume and volume concentration of 50% into the residue, reflux extracting for 1 time at 95deg.C for 1 hr, and recovering 50% ethanol under reduced pressure to obtain extract of 50% ethanol; combining 95% ethanol extract and 50% ethanol extract (5.4 kg), adding 10.8L absolute ethanol for dissolution, adding 10.8kg diatomite for adsorption, recovering solvent, sequentially adding 16.2L dichloromethane, ethyl acetate and methanol for elution, and recovering solvent to obtain dichloromethane elution part, ethyl acetate elution part and methanol elution part respectively; separating methanol elution part (3.4 kg) by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient is prepared by using 60L eluent with flow rate of 70mL/min, each 10L is a fraction, collecting 54 fractions, detecting and analyzing each fraction by silica gel thin layer chromatography, and using GF 254 The thin layer plate is heated for 4min at 105 ℃ by taking petroleum ether-acetone with the volume ratio of 4:5 and dichloromethane-methanol with the volume ratio of 5:2 as developing agents, respectively, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are combined respectively to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 (90.2 g) by sephadex LH-20 gel column chromatography, eluting with 10L of methanol at a flow rate of 10mL/min, collecting 50 fractions each 200mL as one fraction, and respectively combining fractions 1-25, 26-44 and 45-50 by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, a subfraction Fr.M4-2 and a subfraction Fr.M4-3; the subfraction Fr.M4-1 (23.7 g) was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient is 5L, and each gradient is a subcomponent, so that subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating the subfractions Fr.M4-1-2 (4.8 g) by sephadex LH-20 gel column chromatography, eluting with 1000mL of methanol, wherein the flow rate is 5mL/min, each 10mL is one fraction, collecting 100 fractions, respectively combining the fractions 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 by silica gel thin layer chromatography detection analysis, and collecting the fractions to obtain subfractions Fr.M4-1-2-1, subfractions Fr.M4-1-2-2, subfractions Fr.M4-1-2-3, subfractions Fr.M4-1-2-4, subfractions Fr.M4-1-2-5 and subfractions Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 (1.6 g) by preparative high performance liquid chromatography, eluting with se:Sup>A mixed methanol-water solvent with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying subfraction Fr.M4-1-2-3-3 (20.5 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min at flow rate of 3 m/min with YMC-Pack ODS-A chromatography column to obtain dysosmse:Sup>A versipellis E (dysosmaflavonoid E,3.0 mg);
Purifying subfraction Fr.M4-1-2-3-4 (17.5 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with retention time of 30.7min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain dysosmse:Sup>A versipellis ketone F (dysosmaflavonoid F,4.0 mg);
separating the component Fr.M5 (110.0 g) by sephadex LH-20 column chromatography, eluting with 5L of methanol, wherein the flow rate is 10mL/min, and collecting 25 fractions per 200mL, wherein each fraction is subjected to silica gel thin layer chromatography detection analysis, and the fractions are respectively combined with 1-3, 4-5, 6-7, 8-10, 11-13, 14-17, 19-21 and 22-25 to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 (22.9 g) were combined, separated by MCI column chromatography, and purified by MeOH-H in a volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Carrying out gradient elution on an O mixed solvent system, wherein each gradient elution solvent is 2.0L, the flow rate is 5.0mL/min, each 500mL is a fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5, 6-15, 16-23 and 17-28 of the fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 (2.5 g) were separated by column chromatography on silica gel with a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 CHCl 3 Gradient elution is carried out by a MeOH mixed solvent system, wherein each gradient elution solvent is 100mL, the flow rate is 5.0mL/min, each 10mL is one fraction, 80 fractions are collected, each fraction is subjected to silica gel thin layer chromatography detection analysis, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain subfractions Fr.M5-1-3-1, subfractions Fr.M5-1-3-2, subfractions Fr.M5-1-3-3, subfractions Fr.M5-1-4, subfractions Fr.M5-1-3-5, subfractions Fr.M5-1-3-6, subfractions Fr.M5-1-3-8, subfractions Fr.M5-1-3-9; subcomponent Fr.M5-1-3-5 (0.25 g) is purified by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, and collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain dysosmse:Sup>A versipellis A (dysosmaflavonoid A,5.0 mg);
separating the component Fr.M6 (130.0 g) by sephadex LH-20 column chromatography, eluting with 30L of methanol at a flow rate of 10mL/min, collecting 30 fractions each 1L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 (57.3 g) was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient elution solvent is 5L, the flow rate is 6.0mL/min, each 1L is one fraction, 30 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are respectively combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 (5.9 g) by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 250L, the flow rate is 2.0mL/min, and each elution gradient is one flow, so as to obtain subfractions Fr.M6-1-3-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating subfractions Fr.M6-1-3-4 (3.2 g) by sephadex LH-20 gel column chromatography, eluting with 250mL of methanol, wherein the flow rate is 2mL/min, each 10mL is a fraction, collecting 25 fractions, and respectively combining fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying subfraction Fr.M6-1-3-4-2 (0.99 g) by preparative high performance liquid chromatography, eluting with 50:50 methanol-water mixed solvent, collecting YMC-Pack ODS-A with flow rate of 3 m/min, and collecting Chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min are obtained to obtain a subfraction Fr.M6-1-3-4-2-1, a subfraction Fr.M6-1-3-4-2-2 and a subfraction Fr.M6-1-3-4-2-3; purifying subfraction Fr.M6-1-3-4-2-1 (20.6 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C (dysosmaflavonoid C,7.0 mg);
purifying subfraction Fr.M6-1-3-4-2-2 (7.6 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D (dysosmaflavonoid D,3.9 mg);
the subfraction Fr.M6-1-3-4-2-3 (16.5 mg) is purified by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, and collecting chromatographic peak with retention time of 28.5min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain compound dysosmse:Sup>A versipellis B (dysosmaflavonoid B,7.9 mg).
Example 2
The invention relates to a preparation method of flavonol compound dysosma versipellis ketone A-F, which takes 20kg of dysosma versipellis medicinal material as raw material, adds 5 times of ethanol with the weight volume and the volume concentration of 95% of the raw material each time, carries out heating reflux extraction for 3 times, the extraction temperature is 92 ℃, the extraction time is 1.5 hours each time, and decompresses and recovers 95% of ethanol to obtain extract of 95% of ethanol; adding ethanol with weight volume of 5 times and volume concentration of 50% into the residue, reflux extracting for 1 time at 92 deg.C for 1.5 hr, and recovering 50% ethanol under reduced pressure to obtain extract 50% ethanol extract; combining 95% ethanol extract and 50% ethanol extract (2.7 kg), adding 5.4L absolute ethanol for dissolution, adding 5.4kg diatomite for adsorption, recovering solvent, sequentially adding 8.1L dichloromethane, ethyl acetate and methanol for elution, and recovering solvent to obtain dichloromethane elution part, ethyl acetate elution part and methanol elution part respectively; separating methanol eluting part (1.7 kg) by silica gel column chromatographySeparating, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient is subjected to gradient elution by using 30L eluent with the flow rate of 50mL/min, each 5L is one fraction, collecting 54 fractions, and performing detection analysis on each fraction by using silica gel thin layer chromatography (GF) 254 The thin layer plate is heated for 5min at 105 ℃ by taking petroleum ether-acetone with the volume ratio of 4:5 and dichloromethane-methanol with the volume ratio of 5:2 as developing agents, respectively, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are combined respectively to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 (45.0 g) by sephadex LH-20 gel column chromatography, eluting with 5.0L methanol at a flow rate of 5mL/min, collecting 50 fractions each 100mL as one fraction, and respectively combining fractions 1-25, 26-44 and 45-50 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M4-1, subfractions Fr.M4-2 and subfractions Fr.M4-3; the subfraction Fr.M4-1 (11.8 g) was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient is 2.5L, each gradient is a subcomponent, and subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating the subfractions Fr.M4-1-2 (2.4 g) by sephadex LH-20 gel column chromatography, eluting with 500mL of methanol, wherein the flow rate is 2.5mL/min, each 5mL is a fraction, collecting 100 fractions, respectively combining fractions 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 by silica gel thin layer chromatography detection analysis, and collecting subfractions Fr.M4-1-2-1, subfractions Fr.M4-1-2-2, subfractions Fr.M4-1-2-3, subfractions Fr.M4-1-2-4, subfractions Fr.M4-1-2-5 and subfractions Fr.M4-1-2-6; sub-class Purifying component M4-1-2-3 (0.8 g) by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min, and 25.1min respectively, to obtain subfractions Fr.M4-1-2-3-1, subfractions Fr.M4-1-2-3-2, subfractions Fr.M4-1-2-3-3, subfractions Fr.M4-1-2-3-4, subfractions Fr.M4-1-2-3-5, subfractions Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain dysosmse:Sup>A versipellis E (dysosmaflavonoid E,1.5 mg);
purifying subfraction Fr.M4-1-2-3-4 (8.7 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with retention time of 30.7min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain dysosmse:Sup>A versipellis ketone F (dysosmaflavonoid F,2.0 mg);
separating the component Fr.M5 (55.0 g) by sephadex LH-20 column chromatography, eluting with 2.5L of methanol, wherein the flow rate is 5mL/min, taking 100mL as one fraction, collecting 25 fractions, respectively combining 1-3 fractions, 4-5 fractions, 6-7 fractions, 8-10 fractions, 11-13 fractions, 14-17 fractions, 19-21 fractions and 22-25 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 (11.4 g) were combined, separated by MCI column chromatography, and purified by MeOH-H in a volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 1.0L, the flow rate is 2.5mL/min, each 250mL is a fraction, 28 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions, 6-15 fractions, 16-23 fractions and 17-28 fractions are respectively combined to obtain a subfraction Fr.M5-1-1, a subfraction Fr.M5-1-2 and a subfraction FrM5-1-3, subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 (1.2 g) were separated by column chromatography on silica gel with a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 CHCl 3 Gradient elution is carried out by a MeOH mixed solvent system, each gradient elution solvent is 50mL, the flow rate is 2.0mL/min, each 5mL is one fraction, 80 fractions are collected, each fraction is subjected to silica gel thin layer chromatography detection analysis, and 1-10, 11-20, 21-30, 31-40, 41-46, 47-51, 52-60, 61-70 and 71-80 of the fractions are respectively combined to obtain subfractions Fr.M5-1-3-1, subfractions Fr.M5-1-3-2, subfractions Fr.M5-1-3-3, subfractions Fr.M5-1-3-4, subfractions Fr.M5-1-3-5, subfractions Fr.M5-1-3-6, subfractions Fr.M5-1-3-7, subfractions Fr.M5-1-3-8 and subfractions Fr.M5-1-3-9; purifying subfraction Fr.M5-1-3-5 (0.13 g) by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain dysosmse:Sup>A versipellis A (dysosmaflavonoid A,2.5 mg);
Separating the component Fr.M6 (65.0 g) by sephadex LH-20 column chromatography, eluting with 15L methanol at a flow rate of 5mL/min, collecting 30 fractions each 0.5L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain sub-components Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 (28.6 g) was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 2.5L, the flow rate is 3.0mL/min, each 0.5L is one fraction, 30 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; the subfractions Fr.M6-1-3 (2.9 g) were separated by silica gel column chromatography and eluted in a gradient of a dichloromethane-methanol mixed solvent system in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, each gradient eluting solvent being 125L,the flow rate is 1.0mL/min, and each elution gradient is one flow part, so as to obtain a subfraction Fr.M6-1-3-1, a subfraction Fr.M6-1-3-2, a subfraction Fr.M6-1-3-3, a subfraction Fr.M6-1-3-4, a subfraction Fr.M6-1-3-5, a subfraction Fr.M6-1-3-6 and a subfraction Fr.M6-1-3-7; separating subfractions Fr.M6-1-3-4 (1.6 g) by sephadex LH-20 gel column chromatography, eluting with 125mL of methanol at a flow rate of 1mL/min, collecting 25 fractions each of which is one fraction per 5mL, and respectively combining fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 (0.5 g) by preparative high performance liquid chromatography, eluting with se:Sup>A methanol-water mixed solvent with se:Sup>A volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively at se:Sup>A flow rate of 3 m/min for YMC-Pack ODS-A, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying subfraction Fr.M6-1-3-4-2-1 (10.3 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C (dysosmaflavonoid C,3.5 mg);
Purifying subfraction Fr.M6-1-3-4-2-2 (3.8 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D (dysosmaflavonoid D,2.0 mg);
the subfraction Fr.M6-1-3-4-2-3 (8.2 mg) is purified by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, and collecting chromatographic peak with retention time of 28.5min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain compound dysosmse:Sup>A versipellis B (dysosmaflavonoid B,4.0 mg).
Example 3
The invention relates to flavonol compounds dysosma versipellis A-FThe preparation method comprises heating and reflux-extracting 30kg of Dysosma versipellis medicinal material with 4 times of 95% ethanol by weight and volume for 3 times at 93 deg.C for 1.2 hr, and recovering 95% ethanol under reduced pressure to obtain extract 95% ethanol extract; adding 4 times of ethanol with weight volume and volume concentration of 50% into the residue, reflux extracting for 1 time at 93 deg.C for 1.2 hr, and recovering 50% ethanol under reduced pressure to obtain extract 50% ethanol extract; combining 95% ethanol extract and 50% ethanol extract (4.0 kg), adding 8.0L absolute ethanol for dissolution, adding 8.0kg diatomite for adsorption, recovering solvent, sequentially adding 12L dichloromethane, ethyl acetate and methanol for elution, and recovering solvent to obtain dichloromethane elution part, ethyl acetate elution part and methanol elution part respectively; separating methanol elution part (2.5 kg) by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient is prepared by using 45L eluent with flow rate of 60mL/min and each 7.5L is prepared as a fraction, collecting 54 fractions, detecting and analyzing each fraction by silica gel thin layer chromatography, and performing GF detection analysis on each fraction 254 The thin layer plate is heated for 3min at 105 ℃ by taking petroleum ether-acetone with the volume ratio of 4:5 and dichloromethane-methanol with the volume ratio of 5:2 as developing agents, respectively, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are combined to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 (67.6 g) by sephadex LH-20 gel column chromatography, eluting with 7.5L methanol at a flow rate of 7.5mL/min, collecting 50 fractions each 150mL as one fraction, and respectively combining fractions 1-25, 26-44 and 45-50 by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, a subfraction Fr.M4-2 and a subfraction Fr.M4-3; the subfraction Fr.M4-1 (17.7 g) was separated by ODS column chromatography toMeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out on the O mixed solvent system, each gradient is 3.75L, each gradient is a subcomponent, and subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating the subfractions Fr.M4-1-2 (3.6 g) by sephadex LH-20 gel column chromatography, eluting with 750mL of methanol, wherein the flow rate is 4mL/min, each 7.5mL is a fraction, collecting 100 fractions, respectively combining the fractions 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 by silica gel thin layer chromatography detection analysis, and collecting the fractions to obtain subfractions Fr.M4-1-2-1, subfractions Fr.M4-1-2-2, subfractions Fr.M4-1-2-3, subfractions Fr.M4-1-2-4, subfractions Fr.M4-1-2-5 and subfractions Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 (1.2 g) by preparative high performance liquid chromatography, eluting with se:Sup>A mixed methanol-water solvent with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying subfraction Fr.M4-1-2-3-3 (15.3 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min at flow rate of 3 m/min with YMC-Pack ODS-A chromatography column to obtain dysosmse:Sup>A versipellis E (dysosmaflavonoid E,1.6 mg);
Purifying subfraction Fr.M4-1-2-3-4 (13.2 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with retention time of 30.7min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain dysosmse:Sup>A versipellis ketone F (dysosmaflavonoid F,2.2 mg);
separating the component Fr.M5 (82.5 g) by sephadex LH-20 column chromatography, eluting with 3.75L methanol at a flow rate of 7.5mL/min, collecting 25 fractions per 150mL,the components are detected and analyzed by silica gel thin layer chromatography, and 1-3 parts, 4-5 parts, 6-7 parts, 8-10 parts, 11-13 parts, 14-17 parts, 19-21 parts and 22-25 parts are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 (17.1 g) were combined, separated by MCI column chromatography, and purified by MeOH-H in a volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 1.5L, the flow rate is 3.7mL/min, each 375mL is a fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-15 fractions, 16-23 fractions and 17-28 fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 (1.8 g) were separated by column chromatography on silica gel with a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 CHCl 3 Gradient elution of MeOH mixed solvent systems, wherein each gradient elution solvent is 75mL, the flow rate is 4.0mL/min, each 7.5mL is one fraction, 80 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain subfractions Fr.M5-1-3-1, subfractions Fr.M5-1-3-2, subfractions Fr.M5-1-3-3, subfractions Fr.M5-1-3-4, subfractions Fr.M5-1-3-5, subfractions Fr.M5-1-3-6, subfractions Fr.M5-1-3-7, subfractions Fr.M5-1-3-8 and subfractions Fr.M5-1-3-9; purifying subfraction Fr.M5-1-3-5 (0.18 g) by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain dysosmse:Sup>A versipellis A (dysosmaflavonoid A,3.7 mg);
separating Fr.M6 (97.5 g) with sephadex LH-20 column chromatography, eluting with 22.5L methanol at a flow rate of 7.5mL/min, collecting each 0.75L as a fraction30 fractions, wherein each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-18 fractions and 19-30 fractions are respectively combined to obtain subfractions Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 (42.9 g) was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 3.75L, the flow rate is 5.0mL/min, each 0.75L is one fraction, 30 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 (4.4 g) by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 185L, the flow rate is 1.5mL/min, and each elution gradient is one flow, so as to obtain subfractions Fr.M6-1-3-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating subcomponent Fr.M6-1-3-4 (2.4 g) by sephadex LH-20 gel column chromatography, eluting with 175mL of methanol at a flow rate of 1.5mL/min, collecting 25 fractions each 7mL of which is a fraction, and respectively combining fraction 1-7, fraction 8-16 and fraction 17-25 by silica gel thin layer chromatography detection analysis to obtain subcomponent Fr.M6-1-3-4-1, subcomponent Fr.M6-1-3-4-2 and subcomponent Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 (0.74 g) by preparative high performance liquid chromatography, eluting with se:Sup>A methanol-water mixed solvent with se:Sup>A volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively at se:Sup>A flow rate of 3 m/min for YMC-Pack ODS-A, and collecting subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying subfraction Fr.M6-1-3-4-2-1 (15.4 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic column YMC-Pack ODS-A with flow rate of 3 m/min and collecting retention time of 11.9min Chromatographic peaks to give compound dysosma versipellis C (dysosmaflavonoid C,5.2 mg);
purifying subfraction Fr.M6-1-3-4-2-2 (5.7 mg) by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D (dysosmaflavonoid D,2.9 mg);
the subfraction Fr.M6-1-3-4-2-3 (12.4 mg) is purified by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, and collecting chromatographic peak with retention time of 28.5min at flow rate of 3 m/min with YMC-Pack ODS-A to obtain compound dysosmse:Sup>A versipellis B (dysosmaflavonoid B,5.9 mg).
The compounds prepared by the methods in examples 1-3 of the present invention were subjected to IR, UV and nuclear magnetic resonance spectroscopy 1 H-NMR、 13 C-NMR, HSQC, HMBC), high resolution mass spectrometry (HR-ESI-MS) and the like, are identified as dysosma versipellis A (dysosmaflavonoid A), dysosma versipellis B (dysosmaflavonoid B), dysosma versipellis C (dysosmaflavonoid C), dysosma versipellis D (dysosmaflavonoid D), dysosma versipellis E (dysosmaflavonoid E), dysosma versipellis F (dysosmaflavonoid F), respectively. The related maps are shown in figures 2-13. Experiments show that the method is stable and reliable and is easy to operate, and the obtained product has the capability of scavenging DPPH free radicals. The related data are as follows:
1. Structural identification of compounds
TABLE 1 Compounds 1 1 H NMR 13 C NMR data (DMSO-d) 6 )
TABLE 2 Compounds 2 1 H NMR 13 C NMR data (DMSO-d) 6 )
Compound 3 was a yellow amorphous powder. HR-ESI-MS gives an excimer ion peak M/z: [ M+H ]] + 547.1804 (calculated as 547.1810), molecular formula C was determined 27 H 30 O 12 The unsaturation was 13. Infrared spectra showed the presence of carbonyl groups (1653 cm) -1 ) Hydroxy (3367 cm) -1 ) Benzene ring (1608, 1500 cm) -1 ). The ultraviolet spectrum gives the characteristic maximum absorption of flavonols at 249nm, 320nm. 1 H NMR(500MHz,DMSO-d 6 ) In delta H 6.75 (1 h, d, j=2.1 Hz) and δ H 6.48 (1 h, d, j=2.1 Hz) is meta-coupled, i.e. the characteristic hydrogen signal of the flavonol a ring, suggesting a 5, 7-dioxygen substitution. Delta H 6.73 (1 h, d, j=8.4 Hz) and δ H 6.74 (1 h, d, j=8.4 Hz) is ortho-coupled, i.e. characteristic hydrogen signal of flavonol B ring, suggesting 2',3',4' -trisubstituted of B ring. Methoxy signal delta H 3.52 (3H, s). Isopentenyl signal delta H 1.33 (3 h, s), 1.49 (3 h, s), 5.04 (1 h, t, j=6.9 Hz), 3.24 (2 h, j=6.9 Hz). Delta 4.76 (1 h, D, j=7.5 Hz) is the terminal proton signal of glucose, the relative configuration is determined to be beta-type according to the coupling constant of the terminal protons, and the absolute configuration of glucose is shown to be D-type by acid hydrolysis and HPLC analysis. 13 C NMR(125MHz，DMSO-d 6 ) Gives 27 carbon signals in total, and shows an isopentenyl signal delta by combining HSQC map data C 25.9, 122.9, 130.0, 17.5, 25.4; group of beta-glucose carbon signals delta C 104.1, 73.6, 75.7, 69.7, 77.6, 60.8; aromatic methoxy delta C 59.5; a flavonol skeleton, wherein delta C 173.3 is the characteristic signal of the carbonyl group at the 4-position of flavonol. In HMBC profile, beta-glucose end group hydrogen proton signals δ4.76 (1 h, d, j=7.5 Hz) and δ C 158.8 The presence of a remote correlation of the carbon signal at (C-5) suggests that the hydroxyl group at position 5 is glycosylated. Methoxy proton signals δ3.52 (3H, s) and δ C 140.6 The presence of a remote correlation of the carbon signal at (C-3) indicates that the hydroxyl group at position 3 is methylated. Delta from the isopentenyl signal H 3.24 (2h, d, j=6.9 Hz) and δ C 121.3 The carbon signals at (C-1 '), 127.7 (C-2'), 143.4 (C-3 ') are correlated remotely, indicating that the isopentenyl signal is attached to the 2' position of the flavonol B ring. According to 1 H-NMR 13 C-NMR, data were specifically assigned in combination with HSQC, HMBC (see Table 3). Thus, the structure of compound 3 was identified as 2' - (3-methyl-2-butenyl) -7,3',4' -trihydroxy-3-methoxyflavone 5-O-beta-D-glucoside, a novel compound not reported in the literature, designated as dysosma versipellis C (dysosmaflavonoid C).
TABLE 3 Compounds 3 1 H NMR 13 C NMR data (DMSO-d) 6 )
TABLE 4 Compounds 4 1 H NMR 13 C NMR data (DMSO-d) 6 )
TABLE 5 Compounds 5 1 H NMR 13 C NMR data (DMSO-d) 6 )
TABLE 6 Compounds 6 1 H NMR 13 C NMR data (DMSO-d) 6 )
2. Antioxidant Activity
Fully and uniformly mixing DPPH solution (final concentration is 100 mu M) with the sample solution to be tested (final concentration is 6.25,12.5,25,50,100 mu M) with equal volume and different concentrations, standing in dark at room temperature for 1 hour, and measuring at 515nmThe absorbance was determined. IC for DPPH free radical scavenging ability 50 The value represents. The results are shown in Table 7.
TABLE 7 free radical scavenging ability of Compounds 1-6 against DPPH
The experiments show that the novel flavonol compounds prepared by the invention, including dysosma versipellis A (dysosmaflavonoid A), dysosma versipellis B (dysosmaflavonoid B), dysosma versipellis C (dysosmaflavonoid C), dysosma versipellis E (dysosmaflavonoid E) and dysosma versipellis F (dysosmaflavonoid F), have the capability of scavenging DPPH free radicals, and can be used as an antioxidant for food or beverage.
Claims (6)
1. The flavonol compounds dysosma versipellis A-F are dysosma versipellis A (dysosmaflavonoid A), dysosma versipellis B (dysosmaflavonoid B), dysosma versipellis C (dysosmaflavonoid C), dysosma versipellis D (dysosmaflavonoid D), dysosma versipellis E (dysosmaflavonoid E) and dysosma versipellis F (dysosmaflavonoid F) extracted from dysosma versipellis, and the molecular structural formulas are respectively:
2. The method for preparing the flavonol compound dysosma versipellis A-F as claimed in claim 1, which is characterized by comprising the following steps: 20-40kg of dysosma versipellis medicinal material is taken as a raw material, 3-5 times of ethanol with the weight volume and the volume concentration of 95% is added each time, the ethanol is heated and refluxed for extraction for 3 times, the weight volume is calculated by kg of solid, the liquid is calculated by L, the extraction temperature is 92-95 ℃, the extraction time is 1-1.5 hours each time, and the ethanol with the volume concentration of 95% is recovered under reduced pressure to obtain an extract of 95% ethanol; reflux-extracting the residue with 3-5 times of 50% ethanol under heating at 92-95deg.C for 1-1.5 hr, and recovering 50% ethanol under reduced pressure to obtain extractA 50% ethanol extract; mixing 95% ethanol extract and 50% ethanol extract, adding anhydrous ethanol 2 times the weight of the extract for dissolution, adding diatomite 2 times the weight of the extract for adsorption, recovering solvent, sequentially adding dichloromethane, ethyl acetate and methanol 3 times the weight of the extract for elution, and recovering solvent to obtain dichloromethane elution part, ethyl acetate elution part and methanol elution part respectively; separating the methanol elution part by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient uses 30L-60L eluent with the flow rate of 50-70mL/min, each 5L-10L is a fraction, 54 fractions are collected, and each fraction is detected and analyzed by silica gel thin layer chromatography and GF is used 254 The thin layer plate is prepared by respectively taking petroleum ether-acetone with the volume ratio of 4:5 and methylene dichloride-methanol with the volume ratio of 5:2 as developing agents, taking sulfuric acid-ethanol solution with the volume ratio of 10:90 as a developing agent, heating for 3-5min at the temperature of 105 ℃, and respectively combining 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts according to the detection result of thin layer chromatography to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 by sephadex LH-20 gel column chromatography, eluting with 5.0L-10L of methanol at a flow rate of 5-10mL/min, collecting 50 fractions each 100-200 mL as one fraction, and respectively combining 1-25 fractions, 26-44 fractions and 45-50 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, a subfraction Fr.M4-2 and a subfraction Fr.M4-3; the subfraction Fr.M4-1 was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient is 2.5L-5L, each gradient is a subcomponent, and subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; subfraction Fr.M4-1-2 is subjected to sephade Separating by x LH-20 gel column chromatography, eluting with 500-1000mL of methanol at a flow rate of 2.5-5mL/min, collecting 100 fractions each 5mL-10mL as one fraction, and subjecting each fraction to silica gel thin layer chromatography detection analysis to respectively combine 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 fractions to obtain subfractions Fr.M4-1-2-1, subfractions Fr.M4-1-2-2, subfractions Fr.M4-1-2-3, subfractions Fr.M4-1-2-4, subfractions Fr.M4-1-2-5 and subfractions Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 by preparative high performance liquid chromatography, eluting with se:Sup>A mixed solvent of methanol and water with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively for the chromatographic column YMC-Pack ODS-A, the flow rate of 3 m/min, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min respectively, wherein the chromatographic column is YMC-Pack ODS-A, and the flow rate is 3 m/min, to obtain dysosmse:Sup>A versipellis E;
Purifying the subfraction Fr.M4-1-2-3-4 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with flow rate of 3 m/min and retention time of 30.7min to obtain dysosmse:Sup>A versipellis ketone F;
separating the component Fr.M5 by sephadex LH-20 column chromatography, eluting with 2.5L-5L methanol at a flow rate of 5-10mL/min, collecting 25 fractions each 100mL-200mL as one fraction, and respectively combining 1-3, 4-5, 6-7, 8-10, 11-13, 14-17, 19-21 and 22-25 by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 are combined and separated by MCI column chromatographyMeOH-H in a volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Carrying out gradient elution on an O mixed solvent system, wherein each gradient elution solvent is 1.0L-2.0L, the flow rate is 2.5-5.0mL/min, each 250mL-500mL is a fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-15 fractions, 16-23 fractions and 17-28 fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 were separated by column chromatography on silica gel with CHCl in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 3 Gradient elution of a MeOH mixed solvent system, wherein each gradient elution solvent is 50-100 mL, the flow rate is 2.0-5.0mL/min, each 5mL-10mL is one fraction, 80 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain a subfraction Fr.M5-1-3-1, a subfraction Fr.M5-1-3-2, a subfraction Fr.M5-1-3-3, a subfraction Fr.M5-1-3-5, a subfraction Fr.M5-1-3-7, a subfraction Fr.M5-1-3-8, and a subfraction Fr.M5-1-3-9; purifying the subfraction Fr.M5-1-3-5 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain dysosmse:Sup>A versipellis ketone A;
separating the component Fr.M6 by sephadex LH-20 column chromatography, eluting with 15L-30L methanol at a flow rate of 5-10mL/min, collecting 30 fractions each 0.5L-1L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 is separated by ODS column chromatography with MeOH-H in volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 2.5L-5L, the flow rate is 3.0-6.0mL/min, each 0.5L-1L is a flow, 30 flow portions are collected, and each flow isThe parts are subjected to detection analysis by silica gel thin layer chromatography, and 1-5 parts, 6-10 parts, 11-20 parts and 21-30 parts are respectively combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 125L-250L, the flow rate is 1.0-2.0mL/min, and each elution gradient is one flow part, so as to obtain subfractions Fr.M6-1-3-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating the subfractions Fr.M6-1-3-4 by sephadex LH-20 gel column chromatography, eluting with 125mL-250mL of methanol at a flow rate of 1-2mL/min, collecting 25 fractions each of which is 5mL-10mL, and respectively combining the fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively for the chromatographic column YMC-Pack ODS-A and flow rate of 3 m/min, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying the subfraction Fr.M6-1-3-4-2-1 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C;
Purifying the subfraction Fr.M6-1-3-4-2-2 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D;
purifying the subfraction Fr.M6-1-3-4-2-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 28.5min, and collecting the compound dysosmse:Sup>A versipellis ketone B.
3. The preparation method of the flavonol compounds dysosma versipellis ketone A-F, which is characterized in that 40kg of dysosma versipellis medicinal material is taken as a raw material, 3 times of ethanol with the weight, volume and volume concentration of 95% is added into the raw material each time, the extraction temperature is 95 ℃ and the extraction time is 1 hour, and 95% ethanol is recovered under reduced pressure to obtain an extract 95% ethanol extract; adding 3 times of ethanol with weight volume and volume concentration of 50% into the residue, reflux extracting for 1 time at 95deg.C for 1 hr, and recovering 50% ethanol under reduced pressure to obtain extract of 50% ethanol; mixing 95% ethanol extract and 50% ethanol extract, adding 10.8L of absolute ethanol for dissolution, adding 10.8kg of diatomite for adsorption, recovering solvent, sequentially adding 16.2L of dichloromethane, ethyl acetate and methanol for eluting, and recovering solvent to obtain dichloromethane eluting part, ethyl acetate eluting part and methanol eluting part respectively; separating the methanol elution part by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient uses 60L eluent with the flow rate of 70mL/min and each 10L is one fraction, collecting 54 fractions, detecting and analyzing each fraction by silica gel thin layer chromatography, and using GF 254 The thin layer plate is heated for 4min at 105 ℃ by taking petroleum ether-acetone with the volume ratio of 4:5 and dichloromethane-methanol with the volume ratio of 5:2 as developing agents, respectively, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are combined respectively to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
component Fr.M4 via sephadex LSeparating by H-20 gel column chromatography, eluting with 10L methanol at a flow rate of 10mL/min, collecting 50 fractions each 200mL as one fraction, and subjecting each fraction to silica gel thin layer chromatography detection analysis to obtain 1-25 fractions, 26-44 fractions and 45-50 fractions, respectively, to obtain subfractions Fr.M4-1, subfractions Fr.M4-2 and subfractions Fr.M4-3; the subfraction Fr.M4-1 was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient is 5L, and each gradient is a subcomponent, so that subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating the subfractions Fr.M4-1-2 by sephadex LH-20 gel column chromatography, eluting with 1000mL of methanol, taking the flow rate as 5mL/min, taking 10mL as one fraction, collecting 100 fractions, respectively combining 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 of the fractions by silica gel thin layer chromatography detection analysis, and collecting the subfractions Fr.M4-1-2-1, fr.M4-1-2, fr.M4-2-3, fr.M4-1-2-4, fr.M4-1-2-5 and Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 by preparative high performance liquid chromatography, eluting with se:Sup>A mixed solvent of methanol and water with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively for the chromatographic column YMC-Pack ODS-A, the flow rate of 3 m/min, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min respectively, wherein the chromatographic column is YMC-Pack ODS-A, and the flow rate is 3 m/min, to obtain dysosmse:Sup>A versipellis E;
Purifying the subfraction Fr.M4-1-2-3-4 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with flow rate of 3 m/min and retention time of 30.7min to obtain dysosmse:Sup>A versipellis ketone F;
separating the component Fr.M5 by sephadex LH-20 column chromatography, eluting with 5L of methanol, wherein the flow rate is 10mL/min, taking 200mL as one fraction, collecting 25 fractions, respectively combining 1-3 fractions, 4-5 fractions, 6-7 fractions, 8-10 fractions, 11-13 fractions, 14-17 fractions, 19-21 fractions and 22-25 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 are combined, separated by MCI column chromatography and separated by MeOH-H with volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Carrying out gradient elution on an O mixed solvent system, wherein each gradient elution solvent is 2.0L, the flow rate is 5.0mL/min, each 500mL is a fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5, 6-15, 16-23 and 17-28 of the fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 were separated by column chromatography on silica gel with CHCl in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 3 Gradient elution is carried out by a MeOH mixed solvent system, wherein each gradient elution solvent is 100mL, the flow rate is 5.0mL/min, each 10mL is one fraction, 80 fractions are collected, each fraction is subjected to silica gel thin layer chromatography detection analysis, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain subfractions Fr.M5-1-3-1, subfractions Fr.M5-1-3-2, subfractions Fr.M5-1-3-3, subfractions Fr.M5-1-4, subfractions Fr.M5-1-3-5, subfractions Fr.M5-1-3-6, subfractions Fr.M5-1-3-8, subfractions Fr.M5-1-3-9; purifying the subfraction Fr.M5-1-3-5 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent at se:Sup>A volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min and YMC-Pack ODS-A columnObtaining the dysosma versipellis A;
separating the component Fr.M6 by sephadex LH-20 column chromatography, eluting with 30L of methanol at a flow rate of 10mL/min, collecting 30 fractions each 1L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain sub-components Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 is separated by ODS column chromatography with MeOH-H in volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient elution solvent is 5L, the flow rate is 6.0mL/min, each 1L is one fraction, 30 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are respectively combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 250L, the flow rate is 2.0mL/min, and each elution gradient is a flow part, so as to obtain subfractions Fr.M6-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating the subfractions Fr.M6-1-3-4 by sephadex LH-20 gel column chromatography, eluting with 250mL of methanol, wherein the flow rate is 2mL/min, taking 10mL as one fraction, collecting 25 fractions, respectively combining the fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively for the chromatographic column YMC-Pack ODS-A and flow rate of 3 m/min, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; the subfraction Fr.M6-1-3-4-2-1 is purified by preparative high performance liquid chromatography to obtain the body Eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with retention time of 11.9min at flow rate of 3 m/min with YMC-Pack ODS-A chromatography column to obtain compound dysosmse:Sup>A versipellis ketone C;
purifying the subfraction Fr.M6-1-3-4-2-2 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D;
purifying the subfraction Fr.M6-1-3-4-2-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 28.5min, and collecting the compound dysosmse:Sup>A versipellis ketone B.
4. The preparation method of the flavonol compounds dysosma versipellis ketone A-F, which is characterized in that 20kg of dysosma versipellis medicinal material is taken as a raw material, ethanol with the weight, volume and volume concentration of 95% is added into the raw material each time, the ethanol is heated and refluxed and extracted for 3 times, the extraction temperature is 92 ℃, the extraction time is 1.5 hours each time, and 95% ethanol is recovered under reduced pressure to obtain an extract of 95% ethanol; adding ethanol with weight volume of 5 times and volume concentration of 50% into the residue, reflux extracting for 1 time at 92 deg.C for 1.5 hr, and recovering 50% ethanol under reduced pressure to obtain extract 50% ethanol extract; mixing 95% ethanol extract and 50% ethanol extract, adding 5.4L absolute ethanol for dissolving, adding 5.4kg diatomite for adsorption, recovering solvent, sequentially adding 8.1L dichloromethane, ethyl acetate and methanol for eluting, and recovering solvent to obtain dichloromethane eluting part, ethyl acetate eluting part and methanol eluting part respectively; separating the methanol elution part by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient uses 30L of eluent, the flow rate is 50mL/min, each 5L is one fraction, 54 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and GF is used for detecting and analyzing the components by using GF 254 Thin-layer plates, respectively by bodyPetroleum ether-acetone with the volume ratio of 4:5 and methylene dichloride-methanol with the volume ratio of 5:2 are taken as developing agents, sulfuric acid-ethanol solution with the volume ratio of 10:90 is taken as a developing agent, heating is carried out for 5min at 105 ℃, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are respectively combined to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 by sephadex LH-20 gel column chromatography, eluting with 5.0L of methanol at a flow rate of 5mL/min, collecting 50 fractions per 100mL, and respectively combining fractions 1-25, 26-44 and 45-50 by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, subfraction Fr.M4-2 and subfraction Fr.M4-3; the subfraction Fr.M4-1 was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, wherein each gradient is 2.5L, each gradient is a subcomponent, and subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating the subfractions Fr.M4-1-2 by sephadex LH-20 gel column chromatography, eluting with 500mL of methanol, taking each 5mL as a fraction, collecting 100 fractions, respectively combining 1-17, 18-41, 42-59, 60-77, 78-92 and 93-100 by silica gel thin layer chromatography detection analysis, and collecting the subfractions Fr.M4-1-2-1, fr.M4-1-2-2, fr.M4-1-2-3, fr.M4-1-2-4, fr.M4-1-2-5 and Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 by preparative high performance liquid chromatography, eluting with mixed methanol-water solvent with volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min, and 25.1min respectively, to obtain subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, flow rate of 3 m/min, and flow rate of 3 m/min, Subfractions Fr.M4-1-2-3-4, fr.M4-1-2-3-5 and Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min respectively, wherein the chromatographic column is YMC-Pack ODS-A, and the flow rate is 3 m/min, to obtain dysosmse:Sup>A versipellis E;
purifying the subfraction Fr.M4-1-2-3-4 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with flow rate of 3 m/min and retention time of 30.7min to obtain dysosmse:Sup>A versipellis ketone F;
separating the component Fr.M5 by sephadex LH-20 column chromatography, eluting with 2.5L of methanol, wherein the flow rate is 5mL/min, taking each 100mL as one fraction, collecting 25 fractions, respectively combining 1-3 fractions, 4-5 fractions, 6-7 fractions, 8-10 fractions, 11-13 fractions, 14-17 fractions, 19-21 fractions and 22-25 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 are combined, separated by MCI column chromatography and separated by MeOH-H with volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Carrying out gradient elution on an O mixed solvent system, wherein each gradient elution solvent is 1.0L, the flow rate is 2.5mL/min, each 250mL is one fraction, 28 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-5, 6-15, 16-23 and 17-28 of the fractions are respectively combined to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 were separated by column chromatography on silica gel with CHCl in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 3 Gradient elution of MeOH mixed solvent systems, wherein each gradient elution solvent is 50mL, the flow rate is 2.0mL/min, each 5mL is one fraction, 80 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, and,The method comprises the steps of obtaining a subfraction Fr.M5-1-3-1, a subfraction Fr.M5-1-3-2, a subfraction Fr.M5-1-3-3, a subfraction Fr.M5-1-3-4, a subfraction Fr.M5-1-3-5, a subfraction Fr.M5-1-3-6, a subfraction Fr.M5-1-3-7, a subfraction Fr.M5-1-3-8 and a subfraction Fr.M5-1-3-9 according to the following steps of 47-51, 52-60, 61-70 and 71-80; purifying the subfraction Fr.M5-1-3-5 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain dysosmse:Sup>A versipellis ketone A;
Separating the component Fr.M6 by sephadex LH-20 column chromatography, eluting with 15L methanol at a flow rate of 5mL/min, collecting 30 fractions each 0.5L as one fraction, and respectively combining the fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain sub-components Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 is separated by ODS column chromatography with MeOH-H in volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 2.5L, the flow rate is 3.0mL/min, each 0.5L is one fraction, 30 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; separating the subfractions Fr.M6-1-3 by silica gel column chromatography, and performing gradient elution by using a methylene dichloride-methanol mixed solvent system with the volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30, wherein each gradient elution solvent is 125L, the flow rate is 1.0mL/min, and each elution gradient is a flow part, so as to obtain subfractions Fr.M6-1, subfractions Fr.M6-1-3-2, subfractions Fr.M6-1-3-3, subfractions Fr.M6-1-3-4, subfractions Fr.M6-1-3-5, subfractions Fr.M6-1-3-6 and subfractions Fr.M6-1-3-7; separating subfractions Fr.M6-1-3-4 by sephadex LH-20 gel column chromatography, eluting with 125mL methanol at a flow rate of 1mL/min, collecting 25 fractions each 5mL as one fraction, respectively combining fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis, Obtaining a subfraction Fr.M6-1-3-4-1, a subfraction Fr.M6-1-3-4-2 and a subfraction Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively for the chromatographic column YMC-Pack ODS-A and flow rate of 3 m/min, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying the subfraction Fr.M6-1-3-4-2-1 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C;
purifying the subfraction Fr.M6-1-3-4-2-2 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D;
purifying the subfraction Fr.M6-1-3-4-2-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 28.5min, and collecting the compound dysosmse:Sup>A versipellis ketone B.
5. The preparation method of the flavonol compounds dysosma versipellis ketone A-F, which is characterized in that 30kg of dysosma versipellis medicinal material is taken as a raw material, ethanol with the weight, volume and 95% of volume concentration of 4 times of the raw material is added for each time, and is heated and refluxed for extraction for 3 times, the extraction temperature is 93 ℃, the extraction time is 1.2 hours each time, and 95% of ethanol is recovered under reduced pressure to obtain an extract of 95% ethanol; adding 4 times of ethanol with weight volume and volume concentration of 50% into the residue, reflux extracting for 1 time at 93 deg.C for 1.2 hr, and recovering 50% ethanol under reduced pressure to obtain extract 50% ethanol extract; mixing 95% ethanol extract and 50% ethanol extract, dissolving in 8.0L anhydrous ethanol, adsorbing with 8.0kg diatomite, and recovering solventSequentially adding 12L of dichloromethane, ethyl acetate and methanol for eluting, and recovering solvent to obtain dichloromethane eluting part, ethyl acetate eluting part and methanol eluting part respectively; separating the methanol elution part by silica gel column chromatography, sequentially performing gradient elution by using a dichloromethane-methanol mixed solvent system with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 100:30, 100:50 and 0:100, wherein each gradient uses 45L eluent with the flow rate of 60mL/min and each 7.5L is a flow, collecting 54 flow parts, detecting and analyzing each flow part by silica gel thin layer chromatography, and using GF 254 The thin layer plate is heated for 3min at 105 ℃ by taking petroleum ether-acetone with the volume ratio of 4:5 and dichloromethane-methanol with the volume ratio of 5:2 as developing agents, respectively, and according to the detection result of thin layer chromatography, 1-12 parts, 13-24 parts, 25-30 parts, 31-36 parts, 37-38 parts, 39-42 parts, 43-44 parts, 45-48 parts and 49-54 parts are combined to obtain a component Fr.M1, a component Fr.M2, a component Fr.M3, a component Fr.M4, a component Fr.M5, a component Fr.M6, a component Fr.M7, a component Fr.M8 and a component Fr.M9;
separating the component Fr.M4 by sephadex LH-20 gel column chromatography, eluting with 7.5L of methanol at a flow rate of 7.5mL/min, collecting 50 fractions each 150mL as one fraction, and respectively combining fractions 1-25, 26-44 and 45-50 by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M4-1, a subfraction Fr.M4-2 and a subfraction Fr.M4-3; the subfraction Fr.M4-1 was separated by ODS column chromatography with MeOH-H in a volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out on the O mixed solvent system, each gradient is 3.75L, each gradient is a subcomponent, and subcomponent Fr.M4-1-1, subcomponent Fr.M4-1-2, subcomponent Fr.M4-1-3, subcomponent Fr.M4-1-4, subcomponent Fr.M4-1-5 and subcomponent Fr.M4-1-6 are obtained; separating subfraction Fr.M4-1-2 by sephadex LH-20 gel column chromatography, eluting with 750mL methanol at a flow rate of 4mL/min, collecting 100 fractions each 7.5mL as one fraction, respectively combining fraction 1-17, fraction 18-41, fraction 42-59, fraction 60-77, fraction 78-92, and fraction 93-100 by silica gel thin layer chromatography detection analysis, Collecting the obtained subfractions Fr.M4-1-2-1, fr.M4-1-2-2, fr.M4-1-2-3, fr.M4-1-2-4, fr.M4-1-2-5 and Fr.M4-1-2-6; purifying the subfraction M4-1-2-3 by preparative high performance liquid chromatography, eluting with se:Sup>A mixed solvent of methanol and water with se:Sup>A volume ratio of 52:48, collecting chromatographic peaks with retention time of 7.9min, 8.3min, 10.8min, 12.8min, 16.4min and 25.1min respectively for the chromatographic column YMC-Pack ODS-A, the flow rate of 3 m/min, and obtaining subfraction Fr.M4-1-2-3-1, subfraction Fr.M4-1-2-3-2, subfraction Fr.M4-1-2-3-3, subfraction Fr.M4-1-2-3-4, subfraction Fr.M4-1-2-3-5 and subfraction Fr.M4-1-2-3-6; purifying the subfraction Fr.M4-1-2-3-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peaks with retention time of 17.5min respectively, wherein the chromatographic column is YMC-Pack ODS-A, and the flow rate is 3 m/min, to obtain dysosmse:Sup>A versipellis E;
purifying the subfraction Fr.M4-1-2-3-4 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 27:73, collecting chromatographic peak with flow rate of 3 m/min and retention time of 30.7min to obtain dysosmse:Sup>A versipellis ketone F;
Separating the component Fr.M5 by sephadex LH-20 column chromatography, eluting with 3.75L of methanol, wherein the flow rate is 7.5mL/min, taking 150mL as one fraction, collecting 25 fractions, respectively combining 1-3 fractions, 4-5 fractions, 6-7 fractions, 8-10 fractions, 11-13 fractions, 14-17 fractions, 19-21 fractions and 22-25 fractions by silica gel thin layer chromatography detection analysis to obtain a subfraction Fr.M5-1, a subfraction Fr.M5-2, a subfraction Fr.M5-3, a subfraction Fr.M5-4, a subfraction Fr.M5-5, a subfraction Fr.M5-6, a subfraction Fr.M5-7 and a subfraction Fr.M5-8; the subfractions Fr.M5-1 to Fr.M5-5 are combined, separated by MCI column chromatography and separated by MeOH-H with volume ratio of 0:100, 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 1.5L, the flow rate is 3.7mL/min, each 375mL is one fraction, 28 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions and 6-17-percent of fractions are combined respectively15. 16-23 parts and 17-28 parts to obtain a subfraction Fr.M5-1-1, a subfraction Fr.M5-1-2, a subfraction Fr.M5-1-3 and a subfraction Fr.M5-1-4; the subfractions Fr.M5-1-3 were separated by column chromatography on silica gel with CHCl in a volume ratio of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10, 7:1, 3:1 3 Gradient elution of MeOH mixed solvent systems, wherein each gradient elution solvent is 75mL, the flow rate is 4.0mL/min, each 7.5mL is one fraction, 80 fractions are collected, each fraction is subjected to detection analysis by silica gel thin layer chromatography, and 1-10 fractions, 11-20 fractions, 21-30 fractions, 31-40 fractions, 41-46 fractions, 47-51 fractions, 52-60 fractions, 61-70 fractions and 71-80 fractions are respectively combined to obtain subfractions Fr.M5-1-3-1, subfractions Fr.M5-1-3-2, subfractions Fr.M5-1-3-3, subfractions Fr.M5-1-3-4, subfractions Fr.M5-1-3-5, subfractions Fr.M5-1-3-6, subfractions Fr.M5-1-3-7, subfractions Fr.M5-1-3-8 and subfractions Fr.M5-1-3-9; purifying the subfraction Fr.M5-1-3-5 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 55:45, collecting chromatographic peak with retention time of 34.9min at flow rate of 3 m/min with YMC-Pack ODS-A column to obtain dysosmse:Sup>A versipellis ketone A;
separating the component Fr.M6 by sephadex LH-20 column chromatography, eluting with 22.5L of methanol at a flow rate of 7.5mL/min, collecting 30 fractions each 0.75L as one fraction, and respectively combining fractions 1-18 and 19-30 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1 and Fr.M6-2; the subfraction Fr.M6-1 is separated by ODS column chromatography with MeOH-H in volume ratio of 10:90, 30:70, 50:50, 70:30, 90:10, 100:0 2 Gradient elution is carried out by an O mixed solvent system, each gradient elution solvent is 3.75L, the flow rate is 5.0mL/min, each 0.75L is one fraction, 30 fractions are collected, each fraction is detected and analyzed by silica gel thin layer chromatography, and 1-5 fractions, 6-10 fractions, 11-20 fractions and 21-30 fractions are combined to obtain a subfraction Fr.M6-1-1, a subfraction Fr.M6-1-2, a subfraction Fr.M6-1-3 and a subfraction M6-1-4; the subfractions Fr.M6-1-3 are separated by silica gel column chromatography and are mixed with methylene dichloride-methanol mixed solvent systems with volume ratios of 100:0, 100:1, 100:3, 100:5, 100:7, 100:10 and 100:30Gradient elution, wherein each gradient elution solvent is 185L, the flow rate is 1.5mL/min, and each elution gradient is a flow part, so as to obtain a subfraction Fr.M6-1-3-1, a subfraction Fr.M6-1-3-2, a subfraction Fr.M6-1-3-3, a subfraction Fr.M6-1-3-4, a subfraction Fr.M6-1-3-5, a subfraction Fr.M6-1-3-6 and a subfraction Fr.M6-1-3-7; separating the subfractions Fr.M6-1-3-4 by sephadex LH-20 gel column chromatography, eluting with 175mL of methanol, wherein the flow rate is 1.5mL/min, each 7mL is a fraction, collecting 25 fractions, and respectively combining the fractions 1-7, 8-16 and 17-25 by silica gel thin layer chromatography detection analysis to obtain subfractions Fr.M6-1-3-4-1, subfractions Fr.M6-1-3-4-2 and subfractions Fr.M6-1-3-4-3; purifying the subfraction Fr.M6-1-3-4-2 by preparative high performance liquid chromatography, eluting with methanol-water mixed solvent with volume ratio of 50:50, collecting chromatographic peaks with retention time of 33.1min, 41.5min and 45.0min respectively for the chromatographic column YMC-Pack ODS-A and flow rate of 3 m/min, and obtaining subfraction Fr.M6-1-3-4-2-1, subfraction Fr.M6-1-3-4-2-2 and subfraction Fr.M6-1-3-4-2-3; purifying the subfraction Fr.M6-1-3-4-2-1 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 35:65, collecting chromatographic peak with flow rate of 3 m/min and retention time of 11.9min to obtain compound dysosmse:Sup>A versipellis C;
Purifying the subfraction Fr.M6-1-3-4-2-2 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 15.6min to obtain compound dysosmse:Sup>A versipellis D;
purifying the subfraction Fr.M6-1-3-4-2-3 by preparative high performance liquid chromatography, eluting with acetonitrile-water mixed solvent with volume ratio of 30:70, collecting chromatographic peak with flow rate of 3 m/min and retention time of 28.5min, and collecting the compound dysosmse:Sup>A versipellis ketone B.
6. Use of the flavonols dysosma versipellis a-F of claim 1 in the preparation of a food or beverage antioxidant.
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