CN115992054A - Tremella aurantialba strain ZJJE004 and industrial cultivation method thereof - Google Patents

Abstract

The invention discloses a tremella aurantialba strain ZJJE004 and a factory cultivation method thereof, the tremella aurantialba strain ZJJE004Naematelia aurantialba) The microbial strain is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) with a preservation date of 2022, 5 and 12 days, and a preservation number of CGMCC No.40189, and an ITS sequence of the microbial strain is shown as SEQ NO. 1. The industrial cultivation method of the tremella aurantialba strain ZJJE004 adopts a liquid strain one-time inoculation method, tissue grafting blocks are not needed, and the cultivation method is greatly optimizedThe cultivation method is simple and convenient, the fungus growing is fast, the pollution is less, the hypha is strong, the cultivation period of the tremella aurantialba is obviously shortened, and the tremella aurantialba can be picked in 45-55 days; the fruiting rate is high, the fruiting body is uniform in size, the fruiting effect is good, the first crop conversion rate can be higher than 80%, the yield is high, large-scale cultivation can be realized, the application value is high, and the method is suitable for industrial production.

Description

Tremella aurantialba strain ZJJE004 and industrial cultivation method thereof

Technical Field

The invention belongs to the technical field of edible fungi, and particularly relates to a tremella aurantialba strain ZJJE004 and an industrial cultivation method thereof.

Background

"JinzhenNaematelia aurantialba (Bandoni & M. Zang) Millanes &Wedin) belonging to the kingdom fungi, basidiomycetas, tremellomycetes, tremellales, auricularia Bao Geke Naemateliaceae, earpusNaematelia。

The tremella aurantialba and the ductile bacteria such as the ductile bacteria and the ductile bacteria have parasitic or partial symbiotic relationship, so that tremella aurantialba fruiting bodies can be grown after the two bacteria are combined together. Lasiosphaera Seu CalvatiaStereum hirsutum(Willd.) Pers.) belonging to the genus Phanerochaete of the family Coriolus of the genus Thelephora, belonging to the genus Basidiomyces, the order Polyporales, the family CoriolusStereum。

The fruiting body of mature tremella aurantialba is in a shape of golden tremella aurantialba, and its appearance is similar to that of brain, and is also called tremella aurantialba, etc. As a precious edible and medicinal fungus, contains a large amount of amino acids, proteins and mineral elements required by human bodies, and the special tremella aurantialba polysaccharide is one of main active ingredients of tremella aurantialba as a current research hot spot, and has various effects of reducing blood fat, reducing blood sugar, enhancing immunity and the like. As recorded in the compendium of materia medica, the tremella aurantiaca has the effects of relieving cough, reducing sputum, promoting the production of body fluid and quenching thirst and can be used for treating symptoms such as excessive phlegm, asthma, phthisis, deficiency tuberculosis, cough, night sweat and the like.

However, the wild tremella aurantialba has a small yield, and is far from satisfactory to meet the market demand, so that artificial cultivation is necessary. However, the tremella aurantialba strain is unstable due to the particularity of the biological characteristics of tremella aurantialba and the uniqueness of strain production, so that the yield of artificially cultivated tremella aurantialba is low and the yield is uneven.

The invention aims to provide a novel tremella aurantialba strain and a factory cultivation method thereof.

Disclosure of Invention

The first aim of the invention is to provide a novel tremella aurantialba strain ZJJE004, the second aim of the invention is to provide a method for culturing liquid strains of tremella aurantialba strain ZJJE004, and the third aim of the invention is to provide a factory culture method of tremella aurantialba strain ZJJE004.

The first object of the present invention is achieved byNaematelia aurantialba) ZJJE004 is preserved in China general microbiological culture Collection center (CGMCC) for 5-12 days in 2022, and the preservation address is North Xili No.1, 3 in the Korean region of Beijing; the preservation number is CGMCC NO.40189; ITS ITS sequence is shown in SEQ NO.1 of the sequence table.

The second aim of the invention is realized by the culture method of the liquid strain of the tremella aurantialba strain ZJJE004, which comprises the following steps:

1) Preparing a liquid culture medium according to a formula (4-6 g of wheat flour, 2-4g of corn flour, 2-4g of peptone, 15-20g of glucose, 0.5-1g of magnesium sulfate, 1-2g of dipotassium hydrogen phosphate and 1L of water), sterilizing the liquid culture medium under the conditions of 0.1-0.2 MPa of pressure and 121 ℃ for 20-40 min, and cooling to obtain fermentation liquor;

2) Inoculating Mao Renge strain seed liquid into the fermentation liquor according to the proportion of 1%, and inoculating the tremella aurantialba strain ZJJE004 seed liquid again according to the proportion of 1%, wherein the inoculation proportion of the tremella aurantialba and tremella aurantialba is 1:1;

3) Culturing in shaker or fermenter for 8-12 days after inoculation to obtain liquid strain.

The third object of the invention is realized by the method for industrially cultivating the tremella aurantialba strain ZJJE004, which comprises the following steps:

A. preparing culture medium, adjusting water content to 56-62%, packaging into culture bags, sterilizing with high pressure steam at 121deg.C for 120min, and cooling;

B. punching 4 holes on the surface of a cultivation bag, inoculating 5-10ml of the liquid strain in each hole, sealing by pasting a breathable film after inoculation, and performing dark cultivation for 20-25 days under the conditions of 17-20 ℃ and 50-60% of air humidity;

C. when Jin Eryuan base starts to grow out and the breathable film is spread, tearing off the breathable film, and raising the air humidity to 80% -85%, and continuing to culture for 10-15 days at 16-18 ℃;

D. when Jin Eryuan base grows to the size of an egg, the humidity is increased to 90% -95%, and the culture is continued for 7-10 days under illumination at 16-19 ℃;

E. when the tremella aurantialba is long and the petals are opened quickly, the humidity is increased to 95% -100%, and the tremella aurantialba is continued to be cultivated for 5-7 days to be mature through illumination;

F. when golden, the tremella aurantialba can be picked after the tremella aurantialba is ripe when the tremella aurantialba is opened.

The beneficial effects of the invention are as follows:

1. the invention provides a new tremella aurantialba strain ZJJE004 and an industrial cultivation technology thereof, the invention adopts a liquid one-time inoculation method, tissue grafting is not needed, a cultivation method is greatly optimized, the cultivation method is simple and convenient, the fungus growing is fast, the pollution is less, and the hypha is strong (figure 3); the liquid inoculation method is adopted, so that the cultivation period of the tremella aurantialba is obviously shortened, and the tremella aurantialba can be picked in 45-55 days; the fruiting rate is high, the fruiting body is uniform in size (shown in figure 5), the fruiting effect is good, the first crop conversion rate can be higher than 80%, the yield is high, large-scale cultivation can be realized, the application value is high, and the method is suitable for industrial production.

2. The invention also provides an SSR molecular marker of the novel tremella aurantialba strain ZJJE004, the marker combination is 1+3/1+3/2/1/1+4/1/1, and the novel tremella aurantialba strain ZJJJE 004 can be used for rapidly identifying the tremella aurantialba strain ZJJE004, and has the characteristics of cost saving, efficiency improvement, convenience in operation and accurate results.

Drawings

FIG. 1 is a colony chart of the tremella aurantialba strain ZJJE004 of the present invention;

FIG. 2 is a microstructure of the spore of the auricularia auricula strain ZJJE004 of the present invention;

FIG. 3 is a diagram showing a liquid strain of the auricularia auricula strain ZJJJE 004 of the present invention cultured for 6 days;

FIG. 4 is a germination chart of mycelia of the golden fungus strain ZJJE004 of the invention;

FIG. 5 is a diagram showing fruiting bodies of the golden fungus strain ZJJE004 industrial cultivation of the invention in example 6;

FIG. 6 is a diagram of the fruiting body of Auricularia in example 6 of the present invention;

FIG. 7 is a cluster tree of genetic distances UPGMA of 8 tremella aurantialba strains constructed based on SSR molecular markers;

fig. 8 is a diagram of allele relative molecular weight peaks obtained by sequentially detecting the primers JESSR003, JESSR010, JESSR032, JESSR090, JESSR098 and JESSR100 in the tremella aurantialba strain ZJJE004 respectively.

Detailed Description

The invention is described in further detail below with reference to the drawings and examples, but is not limited in any way to any changes or modifications made based on the teachings of the invention, which fall within the scope of the invention.

The golden fungus strain ZJJE004 is collected from the wild golden fungus fruiting body of high Li Gong and preserved in China general microbiological culture Collection center (CGMCC) of 5 months and 12 days of 2022, the preservation address is 1 # 3 of North Chen West Lu in the Chaoyang area of Beijing city, and the preservation number is 40189.

The invention also provides a liquid strain culture medium of the tremella aurantialba strain ZJJE004, which consists of 4-6g of wheat flour, 2-4g of corn flour, 2-4g of peptone, 15-20g of glucose, 0.5-1g of magnesium sulfate, 1-2g of dipotassium hydrogen phosphate and 1L of water.

The invention further provides a method for culturing the liquid strain of the tremella aurantialba strain ZJJE004, which is realized by the following steps:

1) Preparing a liquid culture medium according to the liquid strain culture medium formula, sterilizing for 20-40 min under the pressure of 0.1-0.2 MPa at 121 ℃, and cooling to obtain fermentation liquor;

2) Inoculating the fermentation liquor with a strain of the Phanerochaete at a ratio of 1%, inoculating with a strain of the golden fungus ZJJE004 again at a ratio of 1%, wherein the inoculating ratio of the Phanerochaete to the golden fungus is 1:1;

3) Culturing in shaker or fermenter for 8-12 days after inoculation to obtain liquid strain.

In the step 3, the vibration speed of the shaking table is 800-1000r/min, and the temperature is 20-23 ℃.

The invention further provides a culture medium for cultivated species of the tremella aurantialba strain ZJJE004, which consists of 65-70 parts of wood dust, 15-18 parts of corncob, 13.9-15.4 parts of wheat bran, 0.5-0.7 part of gypsum, 0.5-0.7 part of lime and 0.1-0.2 part of potassium dihydrogen phosphate.

The invention discloses a factory cultivation method of tremella aurantialba strain ZJJE004, which is realized by the following steps:

A. preparing a culture medium according to the formula of the culture medium, adjusting the water content to 56-62%, subpackaging into culture bags, sterilizing with high-pressure steam at 121 ℃ for 120min, and cooling;

B. punching 4 holes on the surface of a cultivation bag, inoculating 5-10ml of liquid strain in each hole, sealing by pasting a breathable film after inoculation, and performing dark cultivation for 20-25 days under the conditions of 17-20 ℃ and 50-60% of air humidity;

C. when Jin Eryuan base starts to grow out and the breathable film is spread, tearing off the breathable film, and raising the air humidity to 80% -85%, and continuing to culture for 10-15 days at 16-18 ℃;

D. when Jin Eryuan base grows to the size of an egg, the humidity is increased to 90% -95%, and the culture is continued for 7-10 days under illumination at 16-19 ℃;

E. when the tremella aurantialba is long and the petals are opened quickly, the humidity is increased to 95% -100%, and the tremella aurantialba is continued to be cultivated for 5-7 days to be mature through illumination;

F. when golden, the tremella aurantialba can be picked after the tremella aurantialba is ripe when the tremella aurantialba is opened.

In the step A, 5-15-55 mushroom bags are adopted as cultivation bags.

The invention also provides a molecular marker combination of the tremella aurantialba strain ZJJE004, which consists of 7 pairs of SSR molecular markers: JESSR003, JESSR010, JESSR032, JESSR075, JESSR090, JESSR098, and JESSR100;

the strain which accords with the SSR allele fragment number combination of 1+3/1+3/2/1/1+4/1/1 is the tremella aurantialba strain ZJJE004.

Example 1 Classification and identification of Strain ZJJJE 004

1. Morphological identification

Colony morphology: the collected mature sporophore spores of the tremella aurantialba strain ZJJE004 are separated, purified and cultured to obtain hyphae, milk white and colony-like yeast forms, and the hyphae are shown in figure 1.

Mycelium morphology: as shown in FIG. 2, the spores are round, contain oil droplets, and many spores begin to germinate and grow to hyphae.

Fruiting body morphology: as shown in figure 6, the mature fruiting body is in the shape of golden yellow petal, the appearance of the fruiting body is similar to that of brain, and the fruiting body is smaller in volume, white and smoother in surface when in primary use; gradually grow to the early maturity stage, the basal part of the ear is wedge-shaped, the upper part of the ear is rugged, distorted and fattened, and the ear is shaped like a brain or irregular split, and the internal tissue is full. Middle to late maturation stage, the split valve is deep and shallow; middle stage, partial split is filled, and partial tissue is soft; the surface is golden yellow after maturation, the internal meat is marbled; the diameter is 10-20 cm, and the thickness is 5-11 cm.

2. Molecular biological identification

Extracting ZJJJE 004 genome DNA by CTAB method, and detecting after GelRed staining after electrophoresis by 1% agarose gel; the extracted total DNA was used as a template, and the sequence of Internal Transcribed Spacer (ITS) was amplified using Mix reagent (Hunan Optimu Biotechnology Co., ltd.) and ITS4 (5 '-TCCTCCGCTTATTGATATGC-3') and ITS5 (5 '-GGAAGTAAAAGTCGTAACAAGG-3') as primers. The PCR amplification system was (25. Mu.L): 2 XMix 12.5. Mu.l, 10. Mu.M primers each 0.5. Mu.l, ddH2O 11. Mu.l, DNA template 0.5. Mu.l. The reaction procedure is: pre-denaturation at 95 ℃ for 5 min, entering 35 amplification cycles: denaturation at 95℃for 40 sec, annealing at 50℃for 40 sec, extension at 72℃for 1 min, extension at 72℃for 10 min, and preservation at 4 ℃; detecting after electrophoresis by 1% agarose gel, to the Optimus of the family Praeparata the technique completes sequencing. The sequencing result is subjected to bidirectional splicing to obtain a sequence (shown as SEQ No. 1). Submitting the sequence to GeneBank using BLAST @ww.ncbi.nlm.nih.gov/BLAST), and performs similarity analysis with each strain sequence in the database, and with the sequences existing in the databaseNaematelia aurantialbaThe corresponding sequence consistency (identifiers) of each strain reaches 93% -100%.

Therefore, the result of BLAST comparison and fruiting body shape identification is combined to obtain the new golden fungus strain ZJJE004 @Naematelia aurantialba (Bandoni & M. Zang) Millanes & Wedin）。

Example 3 preparation of liquid bacterial strain of auricularia auricula strain ZJJE004

The preparation method of the tremella aurantialba strain ZJJE004 liquid strain culture medium is as follows: adding 5g of wheat flour, 3g of corn flour, 3g of peptone, 20g of glucose, 1g of magnesium sulfate and 2g of dipotassium hydrogen phosphate into 1L of water, subpackaging with a 1L triangular flask, preparing 700ml of liquid shake flask, sterilizing at 121 ℃ for 30 minutes, cooling, and placing in a superbacteria table for ultraviolet sterilization for 30 minutes for later use;

the cooled liquid shake flask is respectively inoculated with a sterling fungus strain and a tremella aurantialba ZJJE004 strain into the shake flask according to the proportion of 1 percent (the inoculation proportion of the sterling fungus and tremella aurantialba is 1:1), and the sterling fungus strain and tremella aurantialba ZJJJE 004 strain are 7ml;

and (3) after inoculation, carrying out shake culture for 7 days at a culture temperature of 23 ℃ and a shake speed of 800 r/min. The obtained liquid strain has obvious hypha and high consistency (figure 4), and has faint scent.

Example 4 preparation of liquid bacterial strain of auricularia auricula strain ZJJE004

The preparation method of the tremella aurantialba strain ZJJE004 liquid strain culture medium is as follows: adding 4g of wheat flour, 2g of corn flour, 4g of peptone, 15g of glucose, 0.7g of magnesium sulfate and 1g of dipotassium hydrogen phosphate into 1L of water, subpackaging by using a 1L triangular flask, preparing a 700ml liquid shake flask, sterilizing at 121 ℃ for 30 minutes, cooling, and placing in a superbacteria table for ultraviolet sterilization for 30 minutes for later use;

the cooled liquid shake flask is respectively inoculated with a sterling fungus strain and a tremella aurantialba ZJJE004 strain into the shake flask according to the proportion of 1 percent (the inoculation proportion of the sterling fungus and tremella aurantialba is 1:1), and the sterling fungus strain and tremella aurantialba ZJJJE 004 strain are 7ml;

and (3) after inoculation, carrying out shake culture at a shake temperature of 20 ℃ and a shake speed of 1000r/min for 10 days.

Example 5 preparation of liquid bacterial strain of auricularia auricula strain ZJJE004

A preparation method of a tremella aurantialba strain ZJJE004 liquid strain culture medium comprises the following steps: adding 6g of wheat flour, 4g of corn flour, 2g of peptone, 18g of glucose, 0.5g of magnesium sulfate and 1.5g of dipotassium hydrogen phosphate into 1L of water, subpackaging by using a 1L triangular flask, preparing a 700ml liquid shake flask, sterilizing at 121 ℃ for 30 minutes, cooling, and placing in a superbacteria table for ultraviolet sterilization for 30 minutes for later use;

inoculating seed liquid of the Latifuge strain and the golden fungus ZJJE004 strain into shake flasks (the inoculation ratio of the Latifuge strain to the golden fungus is 1:1) respectively according to the proportion of 1 percent after cooling;

after inoculation, the mixture is subjected to shaking culture for 9 days at a shaking table speed of 900r/min at a culture temperature of 21 ℃.

EXAMPLE 6 industrialized cultivation of golden fungus Strain ZJJE004

1) Preparing cultivation materials: weighing raw materials according to the proportion of cultivation materials of 70 parts of wood dust, 15 parts of corncob, 13.9 parts of wheat bran, 0.5 part of gypsum, 0.5 part of lime and 0.1 part of monopotassium phosphate, adding water, and uniformly stirring to ensure that the water content is 60%;

2) Using 5 x 15 x 55 mushroom bags as cultivation bags; bagging the cultivation material;

3) Sterilizing by high-pressure steam at 121deg.C for 120min; taking out and cooling after sterilization is completed;

4) Cooling the culture medium to about 25 ℃, inoculating liquid, perforating 4 surfaces of the long bags, inoculating 10ml of liquid strain prepared in the example 3 into the holes, sealing by pasting a breathable film after inoculation, and performing dark culture for 22 days at the temperature of 17-20 ℃ and the air humidity of 50-60%;

5) Fruiting management: when Jin Eryuan base starts to grow out and the breathable film is spread, tearing off the breathable film, and raising the air humidity to 80% -85%, and continuing to culture for 10 days at 16-18 ℃;

6) When Jin Eryuan base grows to the size of an egg, the humidity is increased to 90% -95%, and the culture is continued for 10 days under illumination at 16-19 ℃;

7) When the tremella aurantialba is long and the petals are opened quickly, the humidity is increased to 95% -100%, and the tremella aurantialba is cultured continuously for 5 days to be mature through illumination;

8) The golden fungus is picked up in golden yellow, and the ear yield of the strain ZJJE004 is 97% and the first crop conversion rate is 85% after statistics.

EXAMPLE 7 industrialized cultivation of auricularia auricula strain ZJJE004

1) Preparing cultivation materials: weighing raw materials according to the proportion of 80 parts of wood dust, 10 parts of corncob, 14.5 parts of wheat bran, 0.6 part of gypsum, 0.4 part of lime and 0.12 part of monopotassium phosphate, adding water, and uniformly stirring to ensure that the water content is 65%;

the other steps were the same as in example 6.

The ear rate of the strain ZJJJE 004 is 97.5% respectively, and the biological efficiency of one batch is 90% after statistics.

Example 8 identification of Strain ZJJJE 004 Using SSR molecular markers

S1, respectively transferring 8 tremella aurantialba strains ZJJE001, ZJJE002, ZJJJE 003, J-2, J-5, J-6, J-7 and the strains ZJJJE 004 to be detected onto potato dextrose agar solid culture medium, and culturing at 25 ℃ for 15 days and then collecting hyphae;

s2, extracting genome DNA of the mycelium, and detecting the concentration and quality of the total genome DNA by an ultraviolet spectrophotometry;

s3, carrying out PCR (polymerase chain reaction) amplification of SSR markers on the DNA extracted in the step 2 by adopting 7 pairs of SSR molecular markers shown in the table 1;

s4, performing capillary electrophoresis detection on the amplified product obtained in the S3, and analyzing the number and molecular weight of allelic fragments amplified by the SSR primer to obtain serial number combinations of different SSR allelic fragments, wherein the strain which accords with the serial number combination of the SSR allelic fragments as 1+3/1+3/2/1/1+4/1/1 is the golden fungus strain ZJJE004 (figure 8).

TABLE 1 SSR marker primer information

S5, clustering analysis

Based on 7 pairs of polymorphism fragments amplified by SSR, 8 tremella aurantialba strains are subjected to genetic diversity analysis, and based on the genetic distance of Nei, UPGMA cluster trees of the 8 tremella aurantialba strains are constructed (figure 7). As can be seen from FIG. 8, the 7 pairs of primers provided by the invention group the golden fungus ZJJJE 003 and ZJJJE 002 into one group, and other J-2, J-5, J-6 and J-7 strains into one group, and the strain ZJJJE 001 is a single branch and is completely distinguished from other strains. It was demonstrated that strain ZJJJE 004 was specific, unlike the remaining 7 strains. Therefore, the 7 pairs of SSR primer combinations provided by the invention can distinguish the strain ZJJE004 from other 7 different golden fungus strains.

Claims (8)

1. Golden fungus strain ZJJE 004%Naematelia aurantialba) The microbial strain is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) with a preservation date of 2022, 5 and 12 days, and a preservation number of CGMCC No.40189, and an ITS sequence of the microbial strain is shown as SEQ NO. 1.

2. A liquid strain culture medium of tremella aurantialba strain ZJJE004 is characterized by comprising 4-6g of wheat flour, 2-4g of corn flour, 2-4g of peptone, 15-20g of glucose, 0.5-1g of magnesium sulfate, 1-2g of dipotassium hydrogen phosphate and 1L of water.

3. A method for culturing a liquid strain of tremella aurantialba strain ZJJE004 is characterized by comprising the following steps:

1) Preparing a liquid culture medium according to the formula of claim 2, sterilizing the liquid culture medium at the pressure of 0.1-0.2 MPa and the temperature of 121 ℃ for 20-40 min, and cooling to obtain fermentation liquor;

2) Inoculating the fermentation liquor into the seed liquid of the sterculia hirsuta according to the proportion of 1%, and inoculating the seed liquid of the ZJJE004 strain of the tremella aurantialba again according to the proportion of 1%, wherein the inoculation proportion of the sterculia hirsuta and the tremella aurantialba is 1:1;

3) Culturing in shaker or fermenter for 8-12 days after inoculation to obtain liquid strain.

4. The method for culturing a liquid strain of Tremella aurantialba strain ZJJJE 004 according to claim 3, wherein in the step 3, the vibration speed of the shaking table is 800-1000r/min, and the temperature is 20-23 ℃.

5. The culture medium for the cultivated species of the tremella aurantialba strain ZJJE004 is characterized by comprising 65-70 parts of wood dust, 15-18 parts of corncob, 13.9-15.4 parts of wheat bran, 0.5-0.7 part of gypsum, 0.5-0.7 part of lime and 0.1-0.2 part of potassium dihydrogen phosphate.

6. The industrial cultivation method of the tremella aurantialba strain ZJJE004 is characterized by comprising the following steps of:

A. preparing a cultivar culture medium according to the formula of claim 5, adjusting the water content to 56-62%, subpackaging into cultivation bags, sterilizing with high-pressure steam at 121deg.C for 120min, and cooling;

B. punching 4 holes on the surface of a cultivation bag, inoculating 5-10ml of liquid strain in each hole, sealing by pasting a breathable film after inoculation, and performing dark cultivation for 20-25 days under the conditions of 17-20 ℃ and 50-60% of air humidity;

C. when Jin Eryuan base starts to grow out and the breathable film is spread, tearing off the breathable film, and raising the air humidity to 80% -85%, and continuing to culture for 10-15 days at 16-18 ℃;

D. when Jin Eryuan base grows to the size of an egg, the humidity is increased to 90% -95%, and the culture is continued for 7-10 days under illumination at 16-19 ℃;

E. when the tremella aurantialba is long and the petals are opened quickly, the humidity is increased to 95% -100%, and the tremella aurantialba is continued to be cultivated for 5-7 days to be mature through illumination;

F. when golden, the tremella aurantialba can be picked after the tremella aurantialba is ripe when the tremella aurantialba is opened.

7. The method according to claim 6, wherein in the step a, 5×15×55 lentinula edodes bags are used as cultivation bags.

8. A molecular marker combination of tremella aurantialba strain ZJJE004, which is characterized by consisting of 7 pairs of SSR molecular markers: JESSR003, JESSR010, JESSR032, JESSR075, JESSR090, JESSR098, and JESSR100;

the strain which accords with the SSR allele fragment number combination of 1+3/1+3/2/1/1+4/1/1 is the tremella aurantialba strain ZJJE004.

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