CN115975786A

CN115975786A - Fluorescent PCR hybridization instrument

Info

Publication number: CN115975786A
Application number: CN202310059790.XA
Authority: CN
Inventors: 吕建新
Original assignee: Hangzhou Medical College
Current assignee: Hangzhou Medical College
Priority date: 2023-01-18
Filing date: 2023-01-18
Publication date: 2023-04-18

Abstract

The invention discloses a fluorescent PCR hybridization instrument, which comprises a bottom plate, wherein the top end of the bottom plate is fixedly connected with a left side plate and a right side plate which are arranged in parallel, the left side plate and the right side plate are both provided with U-shaped through holes, an incubation mechanism is arranged between the left side plate and the right side plate, the incubation mechanism is rotationally and slidably arranged in the U-shaped through holes, one side of the incubation mechanism is provided with an observation part, the top end of the bottom plate is fixedly connected with a casing, and the left side plate, the right side plate, the incubation mechanism and the observation part are positioned in the casing. The hybridization instrument can improve the switching speed of temperature rise and temperature fall of the hybridization instrument and reduce the abrasion of a target object.

Description

Fluorescent PCR hybridization instrument

Technical Field

The invention belongs to the technical field of hybridization equipment, and particularly relates to a fluorescent PCR hybridization instrument.

Background

PCR equipment has a general principle requirement: rapid heating up, rapid cooling, current common PCR equipment all can realize intensification and cooling basically, but rate of rise of temperature, rate of cooling all receive the restriction of structural principle, consequently it is limited to go up and down temperature efficiency, and common structure is the single heating piece realization and goes up and down the temperature, consequently to the intensification and the cooling of target object, all need go up and down the temperature heating piece earlier, then transmit for the target object through the conduction, because the PCR target object need go up and down the temperature repeatedly, consequently, the heating piece need switch over repeatedly. Common conceivable switching ways are: 1. one block is drawn out from the lower part of the target object, and then the next block is inserted, so that the method has larger abrasion to the target object; 2. under the condition that the target object is fixed, one of the target objects is descended and then extracted; then, one piece is switched and then the fit is raised, which is disadvantageous in shortening the switching time, and therefore, a fluorescent PCR hybridization instrument needs to be designed to solve the above problems.

Disclosure of Invention

The invention aims to provide a fluorescent PCR hybridization instrument, which aims to solve the problems and achieve the aims of increasing the switching speed of temperature rise and temperature fall of the hybridization instrument and reducing the abrasion of a target object.

In order to achieve the purpose, the invention provides the following scheme: fluorescence PCR hybridization appearance, comprising a base plate, bottom plate top fixedly connected with mutual parallel arrangement's left side board, right side board, U type through-hole has all been seted up to left side board, right side board, be provided with incubation mechanism between left side board, the right side board, incubation mechanism rotates and slides and sets up in the U type through-hole, incubation mechanism one side is provided with the observation part, bottom plate top fixedly connected with casing, left side board, right side board, incubation mechanism, observation position in the casing.

Preferably, incubation mechanism includes the mounting panel, mounting panel top fixedly connected with heat insulating board, two incubation dishes of heat insulating board top fixedly connected with, every incubation dish top fixedly connected with constant temperature dish, the mounting panel is double-phase to the lateral wall middle part rotate respectively to be connected with the guiding axle, the guiding axle rotates and slides and sets up in the U type through-hole, two radiating blocks of mounting panel bottom middle part fixedly connected with, the radiating block with incubation dish one-to-one, mounting panel bottom both ends fixedly connected with linear bearing respectively, linear bearing vertically slides and wears to establish on first drive division top.

Preferably, the first driving portion includes a second motor, the second motor is fixedly disposed on a connecting plate fixedly connected between the left side plate and the right side plate, an output shaft of the second motor is fixedly connected with one end of a second lead screw, the second lead screw is in threaded connection with a second nut, a top end of the second nut is fixedly connected with a bottom support plate, the linear bearing and the heat dissipation block are in sliding contact with the bottom support plate, second sliding blocks are respectively and fixedly connected to middle portions of two opposite side walls of the bottom support plate, third sliding rails are respectively and fixedly connected to side walls, close to each other, of the left side plate and the right side plate, and the second sliding blocks are slidably disposed on the third sliding rails.

Preferably, the observation part includes the third motor, the third motor with bottom plate top fixed connection, the first slider of third motor output shaft fixedly connected with, casing inside wall fixedly connected with second slide rail, first slider slides and sets up on the second slide rail, fixedly connected with carousel connecting block on the first slider, the carousel connecting block rotates and is connected with the constant temperature and surveys the CD, the constant temperature is surveyed CD bottom fixedly connected with fourth motor output shaft, the constant temperature is surveyed the CD top and is provided with and surveys the light seat, survey the light seat with left side board, right side board fixed connection, survey light seat top fixedly connected with light path unit, reading unit.

Preferably, one side of the turntable connecting block, which is far away from the incubation mechanism, is provided with a constant temperature fan, and the constant temperature fan is fixedly connected with the inner side wall of the machine shell.

Preferably, the left side plate is far away from the side wall of the right side plate and is fixedly connected with a first motor, a first motor output shaft is fixedly connected with a first lead screw, the first lead screw is in threaded connection with a first nut, the side walls of the left side plate and the right side plate which are far away from each other are respectively and fixedly connected with first slide rails, the two first slide rails are jointly and slidably connected with a bracket, and the first nut is fixedly connected with the bracket.

Preferably, there is the apron in casing roof middle part sliding connection, apron bottom fixed connection has the chip lid, apron top fixedly connected with handle, a casing lateral wall fixedly connected with printer, another lateral wall of casing is provided with switch, communication mouth, ventilation hole, be provided with display screen, operating button on the casing communications wall.

Preferably, the middle part of the bottom end of the bottom plate is fixedly connected with a switching power supply, and the side wall of the right side plate, which is far away from the left side plate, is fixedly connected with a counter and a control panel.

The invention has the following technical effects:

the hybridization instrument can improve the switching speed of temperature rise and temperature drop, does not wear a target object, and can effectively improve the consistency of hybridization results of the same batch by combining the centrifugation function, the incubation function and the observation part.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is an isometric view of the present invention;

FIG. 2 is an isometric view of the present invention;

FIG. 3 is a first isometric view of the internal structure of the present invention;

FIG. 4 is an isometric view of an internal structure of the present invention;

FIG. 5 is an isometric view of the internal structure of the present invention;

FIG. 6 is a schematic view of the structure of the incubation mechanism of the present invention.

Wherein, 1, a shell; 2. a printer; 3. a cover plate; 4. a handle; 5. a display screen; 6. an operation button; 7. a power switch; 8. a communication port; 9. a vent hole; 10. a base plate; 11. a motor driver; 12. a first motor; 13. a first nut; 14. a first lead screw; 15. a first slide rail; 16. a bracket; 17. a left side plate; 18. a second motor; 19. a right side plate; 20. a reading unit; 21. a light measuring seat; 22. a constant temperature fan; 23. a counter; 24. a control panel; 25. a switching power supply; 26. an optical path unit; 27. a connecting plate; 28. a third motor; 29. a second slide rail; 30. a first slider; 31. constant temperature CD; 32. a turntable connecting block; 33. a fourth motor; 34. a second slider; 35. a second nut; 36. a second lead screw; 37. a third slide rail; 38. a linear bearing; 39. a bottom pallet; 40. mounting a plate; 41. a heat insulation plate; 42. an incubation tray; 43. a chip cover; 44. a guide shaft; 45. a heat dissipating block; 46. a thermostatic disc.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Referring to fig. 1-6, the invention provides a fluorescent PCR hybridization instrument, comprising a bottom plate 10, wherein the top end of the bottom plate 10 is fixedly connected with a left side plate 17 and a right side plate 19 which are arranged in parallel, the left side plate 17 and the right side plate 19 are both provided with U-shaped through holes, an incubation mechanism is arranged between the left side plate 17 and the right side plate 19, the incubation mechanism is rotatably and slidably arranged in the U-shaped through holes, one side of the incubation mechanism is provided with an observation part, the top end of the bottom plate 10 is fixedly connected with a casing 1, and the left side plate 17, the right side plate 19, the incubation mechanism and the observation part are positioned in the casing 1.

Further optimize the scheme, incubation mechanism includes mounting panel 40, mounting panel 40 top fixedly connected with heat insulating board 41, two incubation dish 42 of heat insulating board 41 top fixedly connected with, every incubation dish 42 top fixed connection has a constant temperature dish 46, the double-phase opposite side wall middle part of mounting panel 40 rotates respectively and is connected with guiding axle 44, guiding axle 44 rotates and slides and sets up in the U type through-hole, two radiating block 45 of mounting panel 40 bottom middle part fixedly connected with, radiating block 45 and incubation dish 42 one-to-one, mounting panel 40 bottom both ends are fixedly connected with linear bearing 38 respectively, the vertical slip of linear bearing 38 is worn to establish on first drive division top.

Further optimize the scheme, first drive division includes second motor 18, second motor 18 is fixed to be established at left side board 17, on fixed connection's the connecting plate 27 between right side board 19, second motor 18 output shaft fixedly connected with second lead screw 36 one end, threaded connection has second nut 35 on the second lead screw 36, second nut 35 top fixedly connected with bottom plate 39, linear bearing 38, radiating block 45 and bottom plate 39 sliding contact, bottom plate 39 double-phase opposite side wall middle part fixedly connected with second slider 34 respectively, left side board 17, respectively fixedly connected with third slide rail 37 on the mutual lateral wall that is close to of right side board 19, second slider 34 slides and sets up on third slide rail 37.

Further optimize the scheme, the observation part includes third motor 28, third motor 28 and the 10 top fixed connection of bottom plate, the first slider 30 of third motor 28 output shaft fixedly connected with, 1 inside wall fixedly connected with second slide rail 29 of casing, first slider 30 slides and sets up on second slide rail 29, fixedly connected with carousel connecting block 32 on the first slider 30, carousel connecting block 32 rotates and is connected with constant

temperature photometry dish

31, 31 bottom fixedly connected with fourth motor 33 output shaft of constant temperature photometry dish, 31 top of constant temperature photometry dish is provided with photometry seat 21, photometry seat 21 and left panel 17, right side plate 19 fixed connection, photometry seat 21 top fixedly connected with light path unit 26, reading unit 20.

In a further optimized scheme, a constant temperature fan 22 is arranged on one side, far away from the incubation mechanism, of the turntable connecting block 32, and the constant temperature fan 22 is fixedly connected with the inner side wall of the machine shell 1.

Further optimize the scheme, the lateral wall fixedly connected with first motor 12 of right side board 19 is kept away from to left side board 17, first motor 12 output shaft fixedly connected with first lead screw 14, first lead screw 14 threaded connection has first nut 13, the lateral wall that left side board 17, right side board 19 kept away from each other is first slide rail 15 of fixedly connected with respectively, the common sliding connection of two first slide rails 15 has bracket 16, first nut 13 and bracket 16 fixed connection.

Further, the side wall of the left side plate 17 far away from the right side plate 19 is also fixedly connected with a motor driver 11.

Further optimize the scheme, 1 roof middle part sliding connection of casing has

apron

3, and 3 bottom fixed connection of apron have

chip lid

43, 3 top fixedly connected with handles 4 of apron, 1 side wall fixedly connected with printer 2 of casing, 1 another lateral wall of casing are provided with switch 7, communication port 8, ventilation hole 9, are provided with display screen 5, operating button 6 on the 1 communications port tilt wall of casing.

In a further optimized scheme, the middle part of the bottom end of the bottom plate 10 is fixedly connected with a switching power supply 25, and the side wall of the right side plate 19 far away from the left side plate 17 is fixedly connected with a counter 23 and a control plate 24.

The working process of the invention is as follows:

switching and heating:

the second motor 18 rotates to drive the second lead screw 36 to rotate, so as to drive the second nut 35 to move left and right; the second nut 35 is fastened with the bottom bracket plate 39 through a screw, and the bottom bracket plate 39 moves along with the screw. The bottom bracket plate 39 is tightly connected with the second slide block 34 and the third slide rail 37, so that the bottom bracket plate 39 generates linear motion. The bottom supporting plate 39 is connected with the mounting plate 40 through the linear bearing 38, and the mounting plate 40 moves along with the bottom supporting plate 39; thermostatic plate 46, incubation dish 42, heat insulating board 41, mounting panel 40 are respectively through the range upon range of fixed connection of screw, and whole structure will produce the side-to-side motion along with mounting panel 40, and mounting panel 40 passes through screw fastening connection with guiding axle 44, and guiding axle 44 is spacing in the U type groove of left side board 17, right side board 19, and linear bearing 38 supports upper and lower free activity again to make thermostatic plate 46 produce the movement track of U type, realize the switching between two thermostatic plates 46 then.

High-speed separation:

the second motor 18 rotates to move the thermostatic disc 46 to first disengage the incubation disc 42 from the chip. The first motor 12 rotates to drive the first lead screw 14 to rotate, so as to drive the first nut 13 to move left and right; the first nut 13 is screwed on the bracket 16, the first slide rail 15 is mounted on the left side plate 17, and the bracket 16 generates a linear motion. The chip is placed on the bracket 16, and the chip just moves to constant temperature photometric disk 31 top along with the bracket 16, and the third motor 28 rotates afterwards, drives first slider 30 motion, and under the spacing of second slide rail 29, first slider 30 is upwards, drives carousel connecting block 32, constant temperature photometric disk 31 and catches the chip. The first motor 12 is then rotated to return the carriage 16 to the initial position. The fourth motor 33 rotates to drive the constant temperature optical disk 31 to rotate, and the chip is driven by the limit column on the constant temperature optical disk 31 to run at high speed to realize liquid separation.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. Fluorescence PCR hybridization appearance, its characterized in that, including bottom plate (10), bottom plate (10) top fixedly connected with left side board (17), right side board (19) of mutual parallel arrangement, U type through-hole has all been seted up to left side board (17), right side board (19), be provided with incubation mechanism between left side board (17), right side board (19), incubation mechanism rotates and slides and sets up in the U type through-hole, incubation mechanism one side is provided with observation portion, bottom plate (10) top fixedly connected with casing (1), left side board (17), right side board (19), incubation mechanism, observation portion are located in casing (1).

2. The fluorescence PCR hybridization instrument according to claim 1, wherein the incubation mechanism comprises a mounting plate (40), a heat insulation plate (41) is fixedly connected to the top end of the mounting plate (40), two incubation disks (42) are fixedly connected to the top end of the heat insulation plate (41), a constant temperature disk (46) is fixedly connected to the top end of each incubation disk (42), guide shafts (44) are respectively and rotatably connected to the middle portions of two opposite side walls of the mounting plate (40), the guide shafts (44) are rotatably and slidably arranged in the U-shaped through holes, two heat dissipation blocks (45) are fixedly connected to the middle portion of the bottom end of the mounting plate (40), the heat dissipation blocks (45) are in one-to-one correspondence with the incubation disks (42), linear bearings (38) are respectively and fixedly connected to the two ends of the bottom end of the mounting plate (40), and the linear bearings (38) are vertically and slidably arranged at the top end of the first driving portion.

3. The fluorescence PCR hybridization instrument according to claim 2, wherein the first driving part comprises a second motor (18), the second motor (18) is fixedly arranged on a connecting plate (27) fixedly connected between the left side plate (17) and the right side plate (19), an output shaft of the second motor (18) is fixedly connected with one end of a second lead screw (36), a second nut (35) is connected onto the second lead screw (36) in a threaded manner, a bottom supporting plate (39) is fixedly connected to the top end of the second nut (35), the linear bearing (38) and the heat dissipation block (45) are in sliding contact with the bottom supporting plate (39), second sliding blocks (34) are respectively and fixedly connected to the middle parts of two opposite side walls of the bottom supporting plate (39), third sliding rails (37) are respectively and fixedly connected to the side walls of the left side plate (17) and the right side plate (19) close to each other, and the second sliding blocks (34) are slidably arranged on the third sliding rails (37).

4. The fluorescence PCR hybridization instrument according to claim 1, wherein the observation portion comprises a third motor (28), the third motor (28) is fixedly connected with the top end of the bottom plate (10), an output shaft of the third motor (28) is fixedly connected with a first sliding block (30), a second sliding rail (29) is fixedly connected with the inner side wall of the housing (1), the first sliding block (30) is slidably arranged on the second sliding rail (29), a rotating disc connecting block (32) is fixedly connected with the first sliding block (30), a constant temperature measurement optical disc (31) is rotatably connected with the rotating disc connecting block (32), a bottom end of the constant temperature measurement optical disc (31) is fixedly connected with a fourth motor (33) output shaft, an optical measurement seat (21) is arranged above the constant temperature measurement optical disc (31), the optical measurement seat (21) is fixedly connected with the left side plate (17) and the right side plate (19), and an optical path unit (26) and a reading unit (20) are fixedly connected with the top end of the optical measurement seat (21).

5. The fluorescence PCR hybridization instrument according to claim 4, wherein a thermostatic fan (22) is disposed on a side of the turntable connecting block (32) away from the incubation mechanism, and the thermostatic fan (22) is fixedly connected with the inner side wall of the housing (1).

6. The fluorescence PCR hybridization instrument according to claim 1, wherein a first motor (12) is fixedly connected to a side wall of the left side plate (17) far away from the right side plate (19), a first lead screw (14) is fixedly connected to an output shaft of the first motor (12), a first nut (13) is connected to the first lead screw (14) in a threaded manner, first slide rails (15) are respectively fixedly connected to side walls of the left side plate (17) and the right side plate (19) far away from each other, a bracket (16) is connected to the two first slide rails (15) in a sliding manner, and the first nut (13) is fixedly connected to the bracket (16).

7. The fluorescence PCR hybridization instrument according to claim 6, wherein a cover plate (3) is slidably connected to the middle of the top wall of the housing (1), a chip cover (43) is fixedly connected to the bottom end of the cover plate (3), a handle (4) is fixedly connected to the top end of the cover plate (3), a printer (2) is fixedly connected to one side wall of the housing (1), a power switch (7), a communication port (8) and a ventilation hole (9) are arranged on the other side wall of the housing (1), and a display screen (5) and an operation button (6) are arranged on the inclined wall of the housing (1).

8. The fluorescence PCR hybridization instrument according to claim 1, wherein the bottom plate (10) is fixedly connected with a switch power supply (25) in the middle of the bottom end, and the right side plate (19) is fixedly connected with a counter (23) and a control plate (24) on the side wall far away from the left side plate (17).