CN115970054A - Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof - Google Patents
Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof Download PDFInfo
- Publication number
- CN115970054A CN115970054A CN202310017281.0A CN202310017281A CN115970054A CN 115970054 A CN115970054 A CN 115970054A CN 202310017281 A CN202310017281 A CN 202310017281A CN 115970054 A CN115970054 A CN 115970054A
- Authority
- CN
- China
- Prior art keywords
- printing
- silicon nitride
- preparation
- loaded
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 51
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 229910052581 Si3N4 Inorganic materials 0.000 title claims abstract description 36
- 238000010146 3D printing Methods 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 230000007547 defect Effects 0.000 claims abstract description 22
- 108010010803 Gelatin Proteins 0.000 claims abstract description 16
- 229920000159 gelatin Polymers 0.000 claims abstract description 16
- 239000008273 gelatin Substances 0.000 claims abstract description 16
- 235000019322 gelatine Nutrition 0.000 claims abstract description 16
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 16
- 239000007864 aqueous solution Substances 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 13
- 108010022355 Fibroins Proteins 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000011282 treatment Methods 0.000 claims abstract description 6
- 239000006185 dispersion Substances 0.000 claims abstract description 4
- 238000007639 printing Methods 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims 3
- 239000000243 solution Substances 0.000 abstract description 17
- 239000002131 composite material Substances 0.000 abstract description 7
- 229910052710 silicon Inorganic materials 0.000 abstract description 7
- 239000010703 silicon Substances 0.000 abstract description 7
- -1 silicon ions Chemical class 0.000 abstract description 7
- 210000002901 mesenchymal stem cell Anatomy 0.000 abstract description 4
- 230000035876 healing Effects 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 210000000963 osteoblast Anatomy 0.000 abstract description 2
- 230000000975 bioactive effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000011164 ossification Effects 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000001788 irregular Effects 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000010478 bone regeneration Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000000399 orthopedic effect Effects 0.000 description 2
- 230000009818 osteogenic differentiation Effects 0.000 description 2
- DHRLEVQXOMLTIM-UHFFFAOYSA-N phosphoric acid;trioxomolybdenum Chemical compound O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.O=[Mo](=O)=O.OP(O)(O)=O DHRLEVQXOMLTIM-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002354 inductively-coupled plasma atomic emission spectroscopy Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Landscapes
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Abstract
The invention discloses a silicon nitride-loaded 3D printing porous bone scaffold as well as a preparation method and application thereof, belonging to the technical field of prostheses capable of being transplanted to bones or prosthesis materials. The biological ink dispersion liquid is a composite solution formed by mixing a gelatin aqueous solution with the concentration of 4-10 percent and a silk fibroin aqueous solution with the concentration of 2-5 percent g/mL according to the volume ratio of 1. The invention has controllable porous structure, and can slowly release silicon ions to promote the differentiation of mesenchymal stem cells to osteoblasts, enhance the healing of bone defect and realize personalized bone defect treatment.
Description
Technical Field
The invention belongs to the technical field of prostheses capable of being transplanted to bones or prosthesis materials, and particularly relates to a silicon nitride-loaded 3D printing porous bone scaffold and a preparation method and application thereof.
Background
The failure of bone tissue function is one of the main reasons for the decline of life quality, the repair of large-size bone defects is always a clinical problem, the treatment effect of autologous bone transplantation or allogeneic bone transplantation treatment strategies is limited to a great extent due to the defects of donor diseases, limited donors and the like, and the development of ideal bone scaffold grafts has important significance. Silicon nitride has the advantage of promoting bone regeneration as a non-bioactive ceramic, and is widely used for preparing non-degradable orthopedic medical instruments such as spinal fusion cages, joint prostheses and the like as a processing material, but the fact that the silicon nitride is processed into nanoparticles as bioactive substances capable of releasing silicon ions and natural materials such as silk fibroin and gelatin are compounded to form a degradable bioactive scaffold for treating bone defect regeneration is not found. Although the traditional stent preparation process has certain advantages in the aspect of processing and forming, the traditional stent preparation process is not suitable for irregular wounds due to the lack of adaptability to defect sizes, and cannot meet the clinical customized requirements. In view of the wide application of the current 3D printing technology in the aspect of customized orthopedic medical products, the customized bone scaffold capable of releasing silicon ions is hopefully obtained by creatively preparing the 3D printing biological ink from silicon nitride, silk fibroin and gelatin and exploring the labor proportioning optimization and printing process, so that the repairing effect in the aspect of bone defects is improved.
Disclosure of Invention
The invention provides a silicon nitride-loaded 3D printing porous bone scaffold and a preparation method and application thereof, aiming at solving the problems that the existing 3D printing bone scaffold is difficult to individually prepare in irregular bone defect, insufficient in biological activity and the like.
The invention is realized by the following technical scheme.
A porous bone scaffold for 3D printing of load silicon nitride is prepared from gelatin aqueous solution with concentration of 4-10% g/mL and silk fibroin aqueous solution with concentration of 2-5% g/mL through mixing at volume ratio of 1.
A preparation method of a silicon nitride-loaded 3D printing porous bone scaffold comprises the steps of mixing a gelatin aqueous solution with the concentration of 4-10 percent g/mL and a silk fibroin aqueous solution with the concentration of 2-5 percent g/mL into a composite solution according to the volume ratio of 1.
The preparation method has the precooling treatment temperature of 4 ℃ and the precooling treatment time of 30min.
According to the preparation method, the temperature of a printing nozzle is set to be 21 ℃, the balance is carried out for 5min, and the temperature of a printer receiving platform is set to be 4 ℃.
According to the preparation method, the diameter of the printing nozzle is 0.4-0.8mm, the pressure applied to the 3D printing nozzle is 60-500kPa, and the filament outlet distance is 200-1000 microns.
According to the preparation method, the number of layers is 5-10, and the printing speed is 40-60mm/s.
The preparation method needs the biological ink dispersion liquid to be uniform and bubble-free.
An application of a silicon nitride-loaded 3D printing porous bone scaffold in preparation of a bone defect repairing material.
Compared with the prior art, the invention has the following beneficial effects:
aiming at the problems that the traditional porous scaffold is difficult to prepare in an individualized way and insufficient in biological activity in irregular bone defects, the bioactive porous scaffold is prepared by adopting a 3D printing technology, so that silicon ions can be released, osteogenic differentiation of mesenchymal stem cells is promoted, and healing of the bone defects is enhanced, so that individualized bone defect treatment is realized, and the specific advantages are as follows:
(1) In the selection of materials, the biodegradable natural silk fibroin and the gelatin have better biocompatibility. The gelatin has the characteristic of low-temperature molding, and is beneficial to low-temperature 3D printing of materials. The silk fibroin has good mechanical property and can enhance the mechanical strength of the gelatin.
(2) In the preparation process, the 3D printing technology is utilized, so that the personalized preparation of the bracket can be realized; the physical crosslinking method is adopted for curing the bracket, and the preparation method has the characteristic of mild preparation conditions.
(3) Functionally, the prepared scaffold has a uniform and controllable pore size structure, can release silicon ions, promotes the differentiation of mesenchymal stem cells into osteoblasts, enhances the healing of bone defects, and is beneficial to the repair and regeneration of bone defects.
Drawings
FIG. 1 is an observation of a macro-morphology A and a micro-morphology B of a porous bone scaffold according to the present invention;
FIG. 2 is a graph showing the silicon ion release behavior of the porous bone scaffold of the present invention;
FIG. 3 is a graph showing the osteogenic differentiation promoting effect of rBMSCs in the porous bone scaffold according to the present invention;
FIG. 4 is a graph of the effect of printing porous bone scaffolds to promote bone regeneration using hematoxylin & eosin staining evaluation;
fig. 5 is a graph of the effect of printing a porous bone scaffold on promoting bone regeneration using masson trichrome stain evaluation.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable one skilled in the art to practice the present invention, the present invention is further described below with reference to specific examples and drawings, but the examples are not intended to limit the present invention.
The experimental methods and the detection methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials were all commercially available unless otherwise specified.
The invention conception of the invention is as follows:
in order to solve the problems that the traditional porous scaffold is difficult to individually prepare in irregular bone defects and insufficient in biological activity, the invention provides the porous scaffold which is constructed by taking silicon nitride as a bioactive substance, taking silk fibroin and gelatin as biological ink and organically combining a physical crosslinking method through low-temperature 3D printing equipment.
The present invention will be described in detail with reference to the following examples and data.
Example 1
The preparation method of the silicon nitride-loaded 3D printing porous bone scaffold comprises the following steps:
(1) Preparation of biological ink: preparing a gelatin aqueous solution having a concentration of 5% g/mL and a silk fibroin aqueous solution (SF solution) having a concentration of 2.5% g/mL, and mixing them at a volume ratio of 1; subsequently, silicon nitride was added to the composite solution in a proportion of 8% by mass/mL of the mass-to-volume ratio of silicon nitride to the composite solution to form a bio-ink. And (3) placing the ink into a water bath kettle at 37 ℃ and fully stirring to uniformly disperse the silicon nitride, and transferring the ink into a feeding pipe of a printer for later use.
(2) Preparing a silicon nitride-loaded 3D printing porous bone scaffold: and (3) placing the material cylinder filled with the ink into a printing nozzle, pre-cooling the ink for 30min by setting the temperature of the nozzle to be 4 ℃, then setting the temperature of the nozzle to be 21 ℃, setting the temperature of a printing receiving platform to be 4 ℃ at the same time, and carrying out printing after 5min of balance. In the printing process, printing layer by layer from bottom to top, wherein the number of printing layers is 8, the printing speed is 50mm/s, and the diameter of a printing needle head is 0.6mm; the pressure applied to the 3D printing nozzle is 100kPa, and the filament outlet distance is 500 mu m.
Example 2
The preparation method of the silicon nitride-loaded 3D printing porous bone scaffold comprises the following steps:
(1) Preparing biological ink: preparing a gelatin aqueous solution having a concentration of 6% g/mL and an SF solution having a concentration of 3% g/mL, and mixing the two solutions at a volume ratio of 1; subsequently, silicon nitride was added to the composite solution at 4%. And (3) placing the ink into a water bath kettle at 37 ℃, fully stirring to uniformly disperse silicon nitride, and transferring the ink into a feeding pipe of a printer for later use.
(2) Preparing a silicon nitride-loaded 3D printing porous bone scaffold: and (3) placing the material cylinder filled with the ink into a printing nozzle, pre-cooling the ink for 30min by setting the temperature of the nozzle to be 4 ℃, then setting the temperature of the nozzle to be 21 ℃, setting the temperature of a printing receiving platform to be 4 ℃ at the same time, balancing for 5min, and then printing. In the printing process, printing layer by layer from bottom to top, wherein the number of printing layers is 10, the printing speed is 50mm/s, and the diameter of a printing needle head is 0.6mm; the pressure applied to the 3D printing nozzle is 150kPa, and the filament outlet distance is 400 mu m.
Example 3
The preparation method of the silicon nitride-loaded 3D printing porous bone scaffold comprises the following steps:
(1) Preparing biological ink: preparing an aqueous gelatin solution having a concentration of 8% g/mL and an SF solution having a concentration of 4% g/mL, and mixing the two solutions at a ratio of 1; subsequently, silicon nitride was added to the composite solution at 1% g/mL by mass/volume of the silicon nitride to composite solution to form a bio-ink. And (3) placing the ink into a water bath kettle at 37 ℃, fully stirring to uniformly disperse silicon nitride, and transferring the ink into a feeding pipe of a printer for later use.
(2) Preparing a silicon nitride-loaded 3D printing porous bone scaffold: and (3) placing the material cylinder filled with the ink into a printing nozzle, pre-cooling the ink for 30min by setting the temperature of the nozzle to be 4 ℃, then setting the temperature of the nozzle to be 21 ℃, setting the temperature of a printing receiving platform to be 4 ℃ at the same time, and carrying out printing after 5min of balance. In the printing process, printing layer by layer from bottom to top, wherein the number of printing layers is 5, the printing speed is 60mm/s, and the diameter of a printing needle is 0.8mm; the pressure applied to the 3D printing nozzle is 200kPa, and the filament outlet distance is 800 μm.
The properties of the scaffolds prepared in examples 1-3 above are similar, and the structure and properties of the scaffold will be described below by taking example 3 as an example only.
Specifically, the porous bone scaffold loaded with silicon nitride and printed in 3D prepared in the above example 3 was subjected to a performance test as follows:
(1) The macroscopic morphology of the silicon nitride-loaded 3D printed porous bone scaffold was photographed and the microstructure of the scaffold was observed using a scanning electron microscope, as shown in FIG. 1. As can be seen from FIG. 1, the scaffold has a net-shaped porous structure, and the pore channels penetrate through the scaffold, and the pore diameter structure is 643.96 +/-59.25 μm.
(2) And (3) detecting the release performance of the bracket silicon ions:
350mg of the support is weighed and respectively added into a centrifuge tube containing 20mL of PBS, the centrifuge tube is fixed on a vertical mixer for shaking, the whole device is placed in a condition of 37 ℃ for experiment, 12mL of release solution is taken out at specific time points (days 1, 3, 5, 7, 10, 14, 21 and 28), 12mL of fresh PBS is supplemented, the release solution is diluted and filtered by a filter membrane of 0.45 mu m, and the concentration of Si ions in the release solution is measured by an ICP-OES instrument, and the result is shown in figure 2.
The results in FIG. 2 show that: the stent has the capability of releasing Si ions, the concentrations of the Si ions released by the stent on the 1 st day are respectively 8.90 +/-0.18 ppm, and the concentrations of the Si ions released by the stent on the 28 th day are respectively 62.98 +/-00.13 ppm, which shows that the stent can release Si for up to 28 days, and provides guarantee for the development of bone bioactivity of the stent.
(3) And (3) detecting the in-vitro osteogenesis promoting activity of the scaffold:
adding 1mL of 0.1% gelatin into each hole of a 6-hole plate for cell culture, shaking uniformly to cover the bottom surface of the plate, standing for 30min, removing the gelatin, airing for standby use, planting bone marrow mesenchymal stem cells (rBMSCs) from a large source into the 6-hole plate according to the density of 15 ten thousand cells/hole, culturing for 24 hours in a normal culture medium, replacing with an osteogenesis induction culture medium, adding a sterilized scaffold and co-culturing with the cells, replacing a fresh culture medium every three days, inducing for 7 days, then carrying out alkaline phosphatase staining, carrying out alizarin red staining after 14 days, and evaluating the osteogenesis induction effect of the scaffold on the rBMSCs, wherein the result is shown in figure 3.
The results in FIG. 3 show that: the bracket group containing silicon nitride can have red positive coloration which is remarkably advanced by alkaline phosphatase and alizarin, and the bracket group containing silicon nitride can promote the generation of alkaline phosphatase in the early osteogenesis stage and the deposition of calcium matrix in the later osteogenesis stage.
(4) And (3) detection of bone repair promotion in the stent body:
SPF grade males, SD rats weighing 280-320g, established femoral defects 3mm in diameter and 3mm in depth and were divided into 3 groups, including a blank control group without any stent implanted, a stent group without silicon nitride added, and a stent group containing silicon nitride. After operation, the penicillin sodium intramuscular injection is continuously injected for 3 days to resist infection. The materials were taken at two time points of 4 weeks and 8 weeks, and were fixed with 4% paraformaldehyde for 48 hours, followed by 6 weeks of decalcification. Subsequently, paraffin embedding was performed, the sections were sectioned, and the sections were subjected to hematoxylin & eosin staining and masson trichrome staining to evaluate bone defect repair, as shown in fig. 4 and 5.
The results of fig. 4 and 5 show that: after the defect is repaired for 4 weeks, the defect area of the bracket group containing silicon nitride is reduced, and obvious collagen is generated; with the increase of the repair time, at week 8, the defect region containing the silicon nitride scaffold group was further healed, and the newly-grown bone was more complete and rich in a higher collagen component, indicating that the silicon nitride scaffold group can significantly promote the regeneration of the bone defect.
Claims (8)
1. The utility model provides a porous bone scaffold of 3D printing of load silicon nitride which characterized in that: the bio-ink dispersion in the charging tube of the printer was prepared by mixing 4-10% g/mL of gelatin aqueous solution and 2-5% g/mL of silk fibroin aqueous solution in a volume ratio of 1.
2. A preparation method of a silicon nitride-loaded 3D printing porous bone scaffold is characterized by comprising the following steps: mixing a gelatin aqueous solution with the concentration of 4-10 percent g/mL and a silk fibroin aqueous solution with the concentration of 2-5 percent g/mL according to the volume ratio of 1.
3. The method of claim 2, wherein: the pre-cooling treatment temperature is 4 deg.C, and the time is 30min.
4. The production method according to claim 2, characterized in that: the temperature of the printing nozzle is set to be 21 ℃, the balance time is 5min, and the temperature of the printer receiving platform is set to be 4 ℃.
5. The method of claim 2, wherein: the diameter of the printing nozzle is 0.4-0.8mm, the pressure applied to the 3D printing nozzle is 60-500kPa, and the filament outlet distance is 200-1000 μm.
6. The production method according to claim 3, characterized in that: the number of layers to be printed layer by layer is 5-10 layers, and the printing speed is 40-60mm/s.
7. The production method according to claim 2, characterized in that; the bio-ink dispersion needs to be uniform and bubble free.
8. An application of a silicon nitride-loaded 3D printing porous bone scaffold in preparation of a bone defect repairing material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310017281.0A CN115970054A (en) | 2023-01-06 | 2023-01-06 | Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310017281.0A CN115970054A (en) | 2023-01-06 | 2023-01-06 | Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115970054A true CN115970054A (en) | 2023-04-18 |
Family
ID=85975861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310017281.0A Pending CN115970054A (en) | 2023-01-06 | 2023-01-06 | Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115970054A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107158474A (en) * | 2017-05-26 | 2017-09-15 | 山东工业陶瓷研究设计院有限公司 | Photocuring 3D printing dentistry implant slurry and its preparation method and application |
| CN107353036A (en) * | 2017-08-21 | 2017-11-17 | 广东工业大学 | A kind of porous silicon nitride ceramic based on increases material manufacturing technology, its preparation method and its application |
| US20180296343A1 (en) * | 2017-04-18 | 2018-10-18 | Warsaw Orthopedic, Inc. | 3-d printing of porous implants |
| CN110054862A (en) * | 2019-05-17 | 2019-07-26 | 广西慧思通科技有限公司 | A kind of PEEK composite material suitable for 3D printing |
| US20200268929A1 (en) * | 2019-02-22 | 2020-08-27 | Ctl Medical Corporation | Settable silicon nitride cements |
| CN113181429A (en) * | 2021-04-26 | 2021-07-30 | 右江民族医学院附属医院 | Method for preparing plastic long-section bone repair material and bone tissue engineering scaffold with controllable slow release of bioactive factors |
| CN114191617A (en) * | 2021-11-12 | 2022-03-18 | 华中科技大学 | Polyether-ether-ketone implant with controllable drug slow release and preparation method thereof |
| CN114315338A (en) * | 2021-05-24 | 2022-04-12 | 山东大学 | Si3N4/CPP composite ceramic material and preparation method and application thereof |
| US20220118686A1 (en) * | 2020-10-15 | 2022-04-21 | Korea Institute Of Materials Science | Method for manufacturing freestanding 3d printing structure |
-
2023
- 2023-01-06 CN CN202310017281.0A patent/CN115970054A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180296343A1 (en) * | 2017-04-18 | 2018-10-18 | Warsaw Orthopedic, Inc. | 3-d printing of porous implants |
| CN107158474A (en) * | 2017-05-26 | 2017-09-15 | 山东工业陶瓷研究设计院有限公司 | Photocuring 3D printing dentistry implant slurry and its preparation method and application |
| CN107353036A (en) * | 2017-08-21 | 2017-11-17 | 广东工业大学 | A kind of porous silicon nitride ceramic based on increases material manufacturing technology, its preparation method and its application |
| US20200268929A1 (en) * | 2019-02-22 | 2020-08-27 | Ctl Medical Corporation | Settable silicon nitride cements |
| CN110054862A (en) * | 2019-05-17 | 2019-07-26 | 广西慧思通科技有限公司 | A kind of PEEK composite material suitable for 3D printing |
| US20220118686A1 (en) * | 2020-10-15 | 2022-04-21 | Korea Institute Of Materials Science | Method for manufacturing freestanding 3d printing structure |
| CN113181429A (en) * | 2021-04-26 | 2021-07-30 | 右江民族医学院附属医院 | Method for preparing plastic long-section bone repair material and bone tissue engineering scaffold with controllable slow release of bioactive factors |
| CN114315338A (en) * | 2021-05-24 | 2022-04-12 | 山东大学 | Si3N4/CPP composite ceramic material and preparation method and application thereof |
| CN114191617A (en) * | 2021-11-12 | 2022-03-18 | 华中科技大学 | Polyether-ether-ketone implant with controllable drug slow release and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| DONG YUNSHENG ET AL: "Sustained release silicon from 3D bioprinting scaffold using silk/gelatin inks to promote osteogenesis", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 14 February 2023 (2023-02-14), pages 123659 * |
| SEUNGHUN S. LEE ET AL: "Silicon Nitride, a Bioceramic for Bone Tissue Engineering: A Reinforced Cryogel System With Antibiofilm and Osteogenic Effects", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 15 December 2021 (2021-12-15), pages 1 - 18 * |
| XIAOYU DU ET AL: "3D-Printed PEEK/Silicon Nitride Scaffolds with a Triply Periodic Minimal Surface Structure for Spinal Fusion Implants", AMERICAN CHEMICAL SOCIETY, 10 August 2023 (2023-08-10), pages 3319 * |
| 杨强: "LARS韧带联合3D打印假体在肿瘤切除桡侧半腕关节重建术中的应用研究", 中国修复重建外科杂志, 1 June 2022 (2022-06-01), pages 822 - 827 * |
| 邹蓉芳: "数字光处理成型氮化硅陶瓷牙科种植体的制备及其生物安全性的初步评价", 解放军医学院学报, 8 May 2023 (2023-05-08), pages 365 - 371 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zong et al. | Reconstruction of rat calvarial defects with human mesenchymal stem cells and osteoblast-like cells in poly-lactic-co-glycolic acid scaffolds | |
| Ohgushi et al. | Osteogenic capacity of rat and human marrow cells in porous ceramics: Experiments in athymic (nude) mice | |
| CN110408058B (en) | Halloysite composite hydrogel for promoting bone defect repair and preparation method and application thereof | |
| Agacayak et al. | Effects of mesenchymal stem cells in critical size bone defect. | |
| Wang et al. | Development of demineralized bone matrix-based implantable and biomimetic microcarrier for stem cell expansion and single-step tissue-engineered bone graft construction | |
| CN111518755A (en) | Bionic periosteum, periosteum-bone substitute and preparation method | |
| CN107137763B (en) | Vascularized tissue engineering bone and preparation method thereof | |
| CN1537020A (en) | Tissue regenerating base material, implanting material and method of producing the same | |
| Zhang et al. | 3D printed reduced graphene oxide-GelMA hybrid hydrogel scaffolds for potential neuralized bone regeneration | |
| Kim et al. | Comparison of osteogenesis between adipose-derived mesenchymal stem cells and their sheets on poly--caprolactone/-tricalcium phosphate composite scaffolds in canine bone defects | |
| Link et al. | Osteogenic properties of starch poly (ε‐caprolactone)(SPCL) fiber meshes loaded with osteoblast‐like cells in a rat critical‐sized cranial defect | |
| CN114606189A (en) | Acellular spinal cord-GelMA hydrogel composite material bracket for promoting proliferation and differentiation of neural stem cells | |
| CN114214271A (en) | Hard material and cell integrated three-dimensional biological printing method, bone repair functional module and preparation method and application of bone organoid | |
| KR20200012815A (en) | Scaffold for regenerating periodontal tissue containing horse bone-derived nanoceramics and PCL and its manufacturing method | |
| CN101716382B (en) | Preparation method of trinary composite stent of plasmid DNA / fibrin gel / polymer | |
| CN112402697A (en) | Application of Schwann cell-derived vesicles induced by skin precursor cells in construction of tissue engineering nerve graft | |
| CN110743036B (en) | Gallium-containing polycaprolactone/bioglass porous bone repair 3D printing support and application thereof in infectious bone defect repair | |
| CN114042191A (en) | Cell-printed osteogenic functional scaffold and preparation method and application thereof | |
| CN110882424B (en) | Oral cavity guided bone regeneration barrier membrane | |
| CN110639058B (en) | Acicular HA/PBLG porous composite microcarrier material for bone tissue engineering and preparation method thereof | |
| Lu et al. | Protein–inorganic hybrid porous scaffolds for bone tissue engineering | |
| CN115970054A (en) | Silicon nitride-loaded 3D printing porous bone scaffold and preparation method and application thereof | |
| Wang et al. | Study of a new nano-hydroxyapatite/basic fibroblast growth factor composite promoting periodontal tissue regeneration | |
| CN110951685A (en) | Monocyte-derived exosome preparation applied to osteogenic differentiation of mesenchymal stem cells | |
| CN114848895A (en) | 3D printing titanium alloy porous support loaded double-factor shell-core microsphere slow release system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yang Qiang Inventor after: Dong Yunsheng Inventor after: Wang Shufang Inventor after: Wan Jinpeng Inventor before: Yang Qiang Inventor before: Dong Yunsheng Inventor before: Wang Shufen Inventor before: Wan Jinpeng |