CN115960770A - Bacillus licheniformis, microbial inoculum and preparation method thereof - Google Patents
Bacillus licheniformis, microbial inoculum and preparation method thereof Download PDFInfo
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000855 fermentation Methods 0.000 claims abstract description 80
- 230000004151 fermentation Effects 0.000 claims abstract description 80
- 238000004321 preservation Methods 0.000 claims abstract description 6
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- 239000003337 fertilizer Substances 0.000 claims description 27
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- 238000000034 method Methods 0.000 claims description 13
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses bacillus licheniformis, a microbial inoculum and a preparation method thereof. The preservation number of the Bacillus licheniformis (Bacillus licheniformis) BL-1106 is GDMCC No:62323. the bacillus licheniformis has stable performance, is not antagonized by the bacillus licheniformis and fermentation metabolites of the bacillus subtilis and the paecilomyces lilacinus, is resistant to high temperature, acid and alkali, and has good application prospect.
Description
Technical Field
The invention belongs to the field of microbial agents and bacterial fertilizers, and particularly relates to bacillus licheniformis and a microbial agent and a preparation method thereof.
Background
The technology for preparing microbial agents and bacterial fertilizers by utilizing natural microorganisms is a green and environment-friendly biological treatment technology which is rapidly developed internationally in the middle and late stages of the last century. Through the comprehensive application of multiple disciplines, the mixed microbial community is used for decomposing multiphase organic matters in a specific environment, and organic solid wastes are fermented and decomposed into humic acid. The technology is used for fertilizing and improving soil, has the characteristics of economy, practicability and no secondary pollution, and is widely concerned. However, the preparation of microbial agents and bacterial fertilizers seems to be simple, which causes huge mass production of individual farmers or small micro-enterprises in China at present, and microbial strain and raw materials are not screened and conditioned in the production process and are directly piled up for fermentation, so that the production process has the advantages of long fermentation period, low production efficiency, large quantity of mixed bacteria and even a large quantity of harmful pathogenic bacteria, undefined target beneficial bacteria, low use of target beneficial bacteria or low survival rate of a shelf, and serious influence on the quality of products and the safety of users.
Bacillus licheniformis is a common bacterium in soil, and can exist in a spore form under a severe environment; under proper environment, it can exist in growth state. It does not produce toxin, is a safe microorganism without pathogenicity, produces antibacterial active substances in the growth process and has a unique biological oxygen-taking action mechanism. Therefore, the plant pathogenic bacteria can be killed, and the disease resistance of crops can be improved due to better inhibition effect on some plant pathogenic bacteria. Therefore, the development of the microbial agent and the bacterial fertilizer becomes a hot spot of development and research of modern agriculture and green agriculture.
Disclosure of Invention
The invention provides bacillus licheniformis, a microbial inoculum containing the bacillus licheniformis and a preparation method of the bacillus licheniformis, and aims to overcome the defects of long fermentation period, low production efficiency, large quantity of mixed bacteria, low survival rate of a shelf and the like of the microbial inoculum in the prior art.
The invention provides a Bacillus licheniformis (Bacillus licheniformis) BL-1106, wherein the preservation number of the Bacillus licheniformis (Bacillus licheniformis) BL-1106 is GDMCC No:62323.
in a second aspect, the present invention provides a fermentation product obtained by fermentation using Bacillus licheniformis (BL-1106) as described in the first aspect of the present invention.
In a third aspect the present invention provides the use of a Bacillus licheniformis (BL-1106) according to the first aspect of the invention or a fermentation product according to the second aspect of the invention in a microbial fertilizer.
In a fourth aspect, the invention provides a microbial fertilizer, wherein the microbial fertilizer comprises Bacillus licheniformis (BL-1106) according to the first aspect of the invention and/or a fermentation product according to the second aspect of the invention.
Optionally, the microbial fertilizer comprises any one of a microbial inoculant (a microbial inoculum), a composite microbial fertilizer and a bio-organic fertilizer.
Optionally, the viable count content of Bacillus licheniformis (Bacillus licheniformis) BL-1106 in the microbial inoculum is more than or equal to 1200 hundred million CFU/g, for example 1300-2000 hundred million CFU/g.
In a fifth aspect of the present invention, there is provided a method for preparing a microbial fertilizer according to the fourth aspect of the present invention, wherein the method comprises the steps of:
adding seed liquid of the Bacillus licheniformis BL-1106, performing fermentation culture to obtain fermentation liquid, and drying the fermentation liquid to obtain the microbial fertilizer.
Optionally, the culture medium used for preparing the seed solution comprises the following components:
optionally, the seed solution is prepared using a medium having a pH of 7.0-7.4.
Optionally, the culture medium adopted by the fermentation culture comprises the following components:
optionally, the fermentation culture employs a medium having a pH of 7.0-7.4.
Optionally, the conditions of the fermentation culture meet at least one of:
the inoculation amount of the seed liquid is 5-15%;
the number of live spores in the seed liquid is more than or equal to 10 hundred million CFU/ml, such as 20-50 hundred million CFU/ml;
the temperature is 28-36 ℃;
the fermentation period is 60-96h;
the reducing sugar content in the fermentation liquor is 0.8-1.5%;
the rotating speed is 200-600rpm;
the ventilation volume (0.6-1.5) is 1v/vm;
the dissolved oxygen is 45-75%.
Optionally, the pH of the fermentation culture is 7.0-7.6.
Optionally, the drying comprises one or more of the following conditions:
the inlet temperature of the spray tower is 120-180 ℃, the outlet temperature is 35-60 ℃, and the fermentation liquor is preheated for 15-20min at 60-80 ℃ before entering the atomizer of the centrifugal spray tower.
In a sixth aspect, the invention provides the use of a Bacillus licheniformis BL-1106 described in the first aspect of the invention, a fermentation product described in the second aspect of the invention, a microbial fertilizer described in the fourth aspect of the invention or a microbial fertilizer produced by a method according to the fifth aspect of the invention in the preparation of a plant growth promoting product.
In a seventh aspect, the invention provides the use of a Bacillus licheniformis (BL-1106) described in the first aspect of the invention, a fermentation product described in the second aspect of the invention, a microbial fertilizer described in the fourth aspect of the invention or a microbial fertilizer produced by a method according to the fifth aspect of the invention for promoting plant growth.
On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the invention.
The reagents and starting materials used in the present invention are commercially available unless otherwise specified.
The positive progress effects of the invention are as follows:
1. obtaining a high-temperature-resistant, acid-resistant and alkali-resistant Bacillus licheniformis strain (Bacillus licheniformis) GDMCC No:62323.
2. the obtained Bacillus licheniformis (Bacillus licheniformis) GDMCC No:62323 the number of colonies produced by submerged fermentation is increased by more than 30% compared with the number of colonies produced by submerged fermentation of control strain Bacillus licheniformis (Bacillus licheniformis) CICC 10037.
3. The obtained Bacillus licheniformis (Bacillus licheniformis) GDMCC No:62323 the product has high spore recovery rate, and the spore survival rate (recovery rate) can still reach 95% after being preserved for one year under the commodity shelf condition.
4. The obtained Bacillus licheniformis (Bacillus licheniformis) GDMCC No:62323 it is not antagonistic to Bacillus licheniformis, bacillus subtilis and Paecilomyces lilacinus metabolite, and can be mixed with Bacillus subtilis and/or Paecilomyces lilacinus metabolite to form coexisting dominant flora, so as to be beneficial to rapidly and directionally creating dominant micro-ecological environment beneficial to plant growth, inhibiting growth and reproduction of pathogenic microorganism, decomposing and degrading chemical substances in soil, increasing soil granular structure, increasing soil air permeability, generating a large amount of antibacterial and bactericidal substances, preventing and treating soil-borne diseases, reducing plant diseases, promoting plant growth, protecting environment, increasing yield, and reducing carbon.
Biological material preservation information
Bacillus licheniformis (Bacillus licheniformis) BL-1106 has been deposited at the Guangdong province culture Collection (GDMCC) at 25/3 in 2022 at accession number: guangzhou city, first furious Zhonglu No. 100 large yard No. 59 building No. 5, zip code: 510070, the preservation number is: GDMCC No:62323 the culture name is BL-1106 and the taxonomic designation is Bacillus licheniformis (Bacillus licheniformis).
Detailed Description
For the purposes of the present invention, the terms used herein have the following meanings, unless otherwise indicated:
the term "submerged fermentation" refers to a fermentation mode in which a strain is inoculated into a liquid fermentation medium, and sterile air is continuously introduced and stirred.
The term "microbial fertilizer" refers to a product containing living organisms of specific microorganisms, which is applied to agricultural production, and can increase the supply of plant nutrients or promote plant growth, increase yield, and improve quality of agricultural products and agricultural ecological environment through the life activities of the microorganisms contained in the product.
The microbial inoculant is one or several kinds of target microbes, and the microbial inoculant may be produced into live microbial product through industrial production, direct use, concentration, drying or adsorption in carrier, and includes:
a single microbial inoculum: a microbial inoculant made of a microbial strain;
a compound microbial inoculum: a microbial inoculant made of two or more microbial strains which are not antagonistic to each other;
organic material decomposing microbial inoculum: microbial inoculants capable of accelerating decomposition and maturity of various organic materials (including crop straws, livestock and poultry manure, household garbage, municipal sludge and the like);
bioremediation agent: the microbial inoculant can reduce the concentration of harmful substances in the environment, reduce toxicity or be harmless through the growth and metabolic activities of microorganisms.
The compound microbial fertilizer is a viable bacterium product which is formed by compounding a target microorganism with nutrient substances after the target microorganism is industrially produced and proliferated.
The biological organic fertilizer is a living bacterial product which is formed by compounding target microorganisms after industrial production and proliferation with organic materials which are mainly derived from animal and plant residues (such as livestock and poultry manure, crop straws and the like) and are subjected to harmless treatment.
The term "CFU" refers to Colony Forming Units (CFU) and refers to each colony formed after incubation on agar plates for a certain period of time and temperature.
In some embodiments, the viable count content of Bacillus licheniformis (BL-1106) in the microbial inoculum provided by the invention is more than or equal to 1200 hundred million CFU/g, preferably more than or equal to 1300 hundred million CFU/g, such as 1300-2000 hundred million CFU/g, or 1500-2000 million CFU/g, or 1500-1800 million CFU/g.
In some embodiments, the preparation method of the microbial inoculum provided by the invention comprises the following steps:
adding seed liquid of the Bacillus licheniformis BL-1106, performing fermentation culture to obtain fermentation liquid, and drying the fermentation liquid to obtain the microbial inoculum.
Preferably, the culture medium used for preparing the seed solution comprises the following components:
preferably, the culture medium adopted by the fermentation culture comprises the following components:
optionally, the fermentation culture employs a medium having a pH of 7.0 to 7.4.
Optionally, the pH of the fermentation culture is 7.4-7.6.
The following describes the selection and selection under pressure of Bacillus subtilis and Paecilomyces lilacinus fermentation metabolite-antagonistic Bacillus licheniformis strains with high temperature resistance, acid and alkali resistance and industrial production application of the strains by combining with the specific operation method of the invention.
Example 1
Metabolite concentrate mixture preparation:
1. preparing a paecilomyces lilacinus submerged fermentation concentrated solution:
deep fermenting Paecilomyces lilacinus (Paecilomyces lilacinus) CICC 40276 until the number of viable spores in fermentation liquor is 30 hundred million CFU/ml, filtering the fermentation liquor by using a ceramic membrane, concentrating the filtrate by 6 times, and preserving the fermentation concentrated solution in a refrigerator at 4-6 ℃.
2. Preparing a bacillus licheniformis submerged fermentation concentrated solution:
the method comprises the steps of filtering a ceramic membrane of fermentation liquor of viable spores of Bacillus licheniformis (CICC 10037) submerged fermentation liquor of 120 hundred million CFU/ml, concentrating the filtrate by 6 times, and preserving the fermentation concentrated solution in a refrigerator at 4-6 ℃.
3. Preparing a bacillus subtilis submerged fermentation concentrated solution:
carrying out submerged fermentation on Bacillus subtilis CMCC 63501 until the number of viable spores in fermentation liquor is 150 hundred million CFU/ml; filtering the fermentation liquor by a ceramic membrane, concentrating the filtrate by 6 times, and preserving the fermentation concentrated solution in a refrigerator at 4-6 ℃.
4. And uniformly mixing the three fermentation concentrated solutions in equal proportion to obtain a mixed solution of the fermentation concentrated solutions rich in the three bacteria fermentation metabolites, and filtering the mixed solution by a sterilizing filter under the aseptic operation condition to obtain a mixed fermentation concentrated solution.
Example 2
1. And (3) breeding dominant bacillus licheniformis:
bacillus licheniformis (Bacillus licheniformis) CICC10037 is used as an original strain to breed the Bacillus licheniformis which is suitable for normal growth in the environment of high-load pressure enrichment of fermentation metabolites of the Bacillus licheniformis, the Bacillus subtilis and the paecilomyces lilacinus.
1.1, preparation of Bacillus licheniformis (Bacillus licheniformis) CICC10037 slant: bacillus licheniformis (Bacillus licheniformis) CICC10037 was inoculated on slant culture medium and cultured for 36h at 35 deg.C.
1.2, preparing Bacillus licheniformis (Bacillus licheniformis) CICC10037 slant bacterial liquid: and taking 5ml of the metabolite concentrate mixture, adding the mature inclined plane of the bacillus licheniformis, scraping off bacterial lawn colonies, and fully dispersing the bacterial lawn into single bacterial colonies.
1.3, preparing heat-resistant bacterial liquid: adding 9ml of the metabolite concentrate mixture into 1ml of the slant bacteria solution to form 10 -1 The diluted sample is stored for 30min in a constant temperature water bath box at 70 ℃.
1.4, separating and breeding strains by using a plate:
from 70 ℃ constant temperature water bath box 10 -1 The diluted tubes were sampled at 0min, 5 min, 10 min, 15 min, 20min, 25 min and 30min, respectively, and further diluted with the metabolite concentrate mixture to give 10 -2 -10 -9 The sample of (4). Respectively taking the dilution 10 -3 To 10 -9 1ml of the sample (2) was plated on a double plate and incubated at 35 ℃ for 36 hours.
The temperature-resistant and antagonistic-resistant plate colony breeding data are as follows:
1.5, acid-resistant and alkali-resistant plate colony breeding:
selecting the above-mentioned antagonism-resistant and 70 deg.C-resistant for 20min (dilution 10) -5 ) 25 min (dilution 10) -4 ) 30 minutes (dilution 10) -4 ) The three bacterial colonies (marked as T20, T25 and T30 respectively) are further subjected to acid-resistant plate (10 g of peptone, 3g of beef extract, 5g of sodium chloride, 10g of soluble powder, 15-20g of agar and 1000ml of distilled water, pH adjustment 4.5, sterilization at 121 ℃ for 30 minutes, split charging into 90mm double disks to prepare separation double disk plates) and alkali-resistant plate (10 g of peptone, 3g of beef extract, 5g of sodium chloride, 10g of soluble powder, 15-20g of agar, 1000ml of distilled water, pH adjustment 9.5, sterilization at 121 ℃ for 30 minutes, split charging into 90mm double disks to prepare separation double disk plates) for breeding, and 1 strain which is selected and is respectively marked as NST20, NST25 and NST30.
2. Sand and soil conservation strain:
respectively preparing three bacillus licheniformis strains of NST20, NST25 and NST30 into bacillus licheniformis suspension; and adding the mixture into a sand soil pipe, and marking the sand soil pipe with a corresponding bacteria number and time. The sandy soil pipe is dried in vacuum, wrapped by kraft paper, placed in a drier and stored in a refrigerator at 4-6 ℃.
Example 3
And (3) stability test:
1. taking one sandy soil pipe from three strains of Bacillus licheniformis NST20, NST25 and NST30 which are preserved for 60 days by the sandy soil pipe, and carrying out shake flask fermentation culture by taking an original strain of Bacillus licheniformis (CICC 10037) as a control to verify the stability of the strains.
1.1, shake flask seed culture medium (wt%): 3.0 parts of soybean meal, 2.0 parts of soybean peptone, 0.3 part of sodium chloride, 2.0 parts of glucose and 1.0 part of corn starch, and the pH value is adjusted to 7.2.
And (3) shake flask seed culture conditions: in a 250ml triangular flask, 25ml of the liquid is filled, the mixture is dug and inoculated, the mixture is shaken on a shaking table with the rotating speed of 200rpm, the mixture is cultured for 48 hours at the temperature of 35 +/-1 ℃, and the number of the live spores is about 30 hundred million CFU/ml by liquid shaking flask fermentation.
1.2, shake flask fermentation medium (wt%): 2.0 parts of glucose, 1.0 part of corn steep liquor, 0.5 part of yeast extract, 3.0 parts of soybean meal, 0.5 part of beef extract, 2.0 parts of corn starch, 0.3 part of sodium chloride, 0.2 part of monopotassium phosphate, 0.3 part of dipotassium phosphate, 0.05 part of magnesium sulfate, 0.15 part of ammonium sulfate and 0.03 part of manganese sulfate, and the pH value is adjusted to be 7.2.
First set of shake flask fermentation culture conditions: adding 40ml shake flask fermentation medium and 5ml metabolite concentrate into 500ml triangular flask, inoculating 5ml, shaking on shaking table rotating at 200rpm, culturing at 35 + -1 deg.C for 84h, and shaking 3 shake flasks in each group.
The second group of shake flask fermentation culture conditions: adding 45ml of shake flask fermentation medium into a 500ml triangular flask, inoculating 5ml of the shake flask fermentation medium, shaking on a shaker rotating at 200rpm, culturing at 35 +/-1 ℃ for 84h, and shaking 3 shake flasks in each group.
2. The average data for each set of 3 shake flask validations was as follows:
from the results, after the bacillus licheniformis strains are preserved for 60 days by sand tube, the number of shake flask culture colonies of three strains of bacillus licheniformis NST20, NST25 and NST30 is higher than that of the original strain CICC10037 of the control group. It can be seen that NST20, NST25 and NST30 have a high stability and a greatly increased tolerance to fermentation products of bacillus subtilis, bacillus licheniformis and paecilomyces lilacinus, and that the growth vigor is not significantly reduced in the shake flask fermentation medium containing the metabolite concentrate compared to the shake flask fermentation medium without the metabolite concentrate, and that the NST30 has the highest growth concentration.
Example 4
1. Production verification:
production verification is carried out by taking a sandy soil pipe of each of the Bacillus licheniformis NST20, NST25 and NST30 strains which are subjected to stability test and taking an original strain Bacillus licheniformis (Bacillus licheniformis) CICC10037 as a control strain.
1.1, seeding tank culture medium (wt%): 3.0 parts of soybean meal, 2.0 parts of soybean peptone, 0.3 part of sodium chloride, 2.0 parts of glucose, 1.0 part of corn starch and the balance of water, and the pH value is adjusted to 7.2.
Seeding tank culture conditions: the rotating speed is 220rpm, the ventilation rate is 1v/vm, the culture is carried out for 48 hours at the temperature of 34 +/-1 ℃, and the number of live spores in seed liquid is about 30 hundred million CFU/ml.
1.2, fermenter culture medium (wt%): 2 parts of glucose, 1.0 part of corn steep liquor, 0.5 part of yeast extract, 3.0 parts of soybean meal, 0.5 part of beef extract, 2.0 parts of corn starch, 0.3 part of sodium chloride, 0.2 part of monopotassium phosphate, 0.3 part of dipotassium phosphate, 0.05 part of magnesium sulfate, 0.15 part of ammonium sulfate, 0.03 part of manganese sulfate, 0.3 part of light calcium carbonate and the balance of water, and the pH is adjusted to be 7.2.
Fermentation culture conditions of a fermentation tank: the inoculation amount is 10 percent, the temperature is 32 ℃, the period is 84 hours, feeding is carried out in the middle of 15 hours to 74 hours, the reducing sugar is ensured to be 1.1 +/-0.2 percent, the rotating speed is 300rpm, the aeration rate is 1v/vm, the dissolved oxygen in the culture medium is controlled to be 60 +/-5 percent, and the pH is controlled to be 7.5 +/-1.0.
1.3, fermentation production data:
from the above results, the colony counts of NST20, NST25 and NST30 were all higher than the control group CICC 10037. NST20, NST25 and NST30 are seen to have higher production activity. Compared with a control group CICC10037, the number of colonies is improved by more than 20%, and the number of NST30 colonies is the highest and is improved by more than 30%.
2. Spray drying:
2.1, adding 0.2% of manganese sulfate, 0.2% of potassium sulfate, 0.3% of magnesium sulfate and 0.5% of light calcium carbonate into the production fermentation liquor of each strain of the bacillus licheniformis, adjusting the pH value to 7.2, and uniformly stirring.
2.2, adjusting the inlet temperature of the spray tower to be 150 +/-5 ℃, the outlet temperature to be 55 +/-5 ℃, and preheating the bacillus licheniformis fermentation liquor for 20min at the temperature of 70 +/-2 ℃ before entering the spray tower.
2.3, uniformly mixing the bacillus licheniformis spray products (microbial agents) with various bacterial numbers respectively, wherein the measurement data is as follows:
it can be seen that NST30 is the most resistant to high temperatures and that the colony count of the product obtained after spray drying is the highest.
Example 5
And (3) commodity shelf stability test:
1. the packaging of each bacterial product of the bacillus licheniformis adopts aluminum plastic vacuum packaging, each bag is 1000g, the products are stored under the conditions that the temperature is less than or equal to 20 ℃, the relative humidity is less than or equal to 65 percent and the sunlight is not directly radiated, and data detection is carried out once every 3 months for 12 months.
2. Shelf stability data are as follows:
from the above results, the survival rates (i.e. recovery rates) of the bacteria after 12-month detection of NST20, NST25 and NST30 are all higher than that of control group cic 10037, and the survival rates all reach more than 92%. It can be seen that NST20, NST25 and NST30 have high shelf stability, NST30 is the most excellent, and the survival rate reaches 95%.
In conclusion, the method selects and breeds the high-temperature-resistant, acid-resistant and alkali-resistant bacillus licheniformis strains which are not antagonized by fermentation metabolites of bacillus subtilis and paecilomyces lilacinus through pressure, has good stability and activity, long shelf life and high production activity, and is beneficial to industrial production and application. NST30 with the best performance is named as Bacillus licheniformis BL-1106 and sent to the Guangdong province microorganism strain preservation center for preservation.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, so that any changes or modifications made in the claims and the specification of the present invention should fall within the scope of the present invention.
Claims (10)
1. Bacillus licheniformis (Bacillus licheniformis) BL-1106, characterized in that the preservation number of the Bacillus licheniformis (Bacillus licheniformis) BL-1106 is GDMCC No:62323.
2. a fermentation product obtained by fermentation using the Bacillus licheniformis (BL-1106) as described in claim 1.
3. Use of Bacillus licheniformis (BL-1106) according to claim 1 or of a fermentation product according to claim 2 in microbial fertilizers.
4. A microbial fertilizer, characterized in that it comprises Bacillus licheniformis (BL-1106) of claim 1 and/or a fermentation product of claim 2.
5. The microbial fertilizer according to claim 4, wherein the microbial fertilizer is a microbial inoculum, and the viable count content of Bacillus licheniformis (Bacillus licheniformis) BL-1106 in the microbial inoculum is more than or equal to 1200 hundred million CFU/g, such as 1300-2000 hundred million CFU/g.
6. A process for the preparation of a microbial fertilizer according to claim 4 or 5, comprising the steps of:
adding seed liquid of the Bacillus licheniformis (BL-1106) and carrying out fermentation culture to obtain fermentation liquid, and drying the fermentation liquid to obtain the microbial fertilizer.
7. The method of claim 6, wherein the fermentation medium comprises the following components:
the balance being water.
8. The method of claim 6, wherein the conditions of the fermentation culture satisfy at least one of:
the inoculation amount of the seed liquid is 5-15%;
the number of live spores in the seed liquid is more than or equal to 10 hundred million CFU/ml, such as 20-50 hundred million CFU/ml;
the temperature is 28-36 ℃;
the fermentation period is 60-96h;
the content of reducing sugar in the fermentation liquor is 0.8-1.5%;
the rotating speed is 200-600rpm;
the ventilation volume (0.6-1.5) is 1v/vm;
the dissolved oxygen is 45-75%.
9. Use of Bacillus licheniformis (BL-1106) according to claim 1, a fermentation product according to claim 2, a microbial fertilizer according to claim 4 or 5 or a microbial fertilizer produced according to a process according to any of claims 6-8 for the production of a plant growth promoting product.
10. Use of Bacillus licheniformis (BL-1106) according to claim 1, a fermentation product according to claim 2, a microbial fertilizer according to claim 4 or 5 or a microbial fertilizer produced according to a process according to any of claims 6-8 for promoting plant growth.
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| CN109468249A (en) * | 2018-12-26 | 2019-03-15 | 佛山市艳晖生物科技有限公司 | A kind of microbial bacterial agent and its application on livestock and poultry cultivation |
| CN110283752A (en) * | 2019-07-11 | 2019-09-27 | 中国科学院青岛生物能源与过程研究所 | The production method and product of the culture medium and production method of bacillus licheniformis, bacillus licheniformis opportunistic pathogen powder |
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| CN101891548A (en) * | 2010-07-21 | 2010-11-24 | 神州汉邦（北京)生物技术有限公司 | Multiple-effect microbial fertilizer prepared by three strains of bacillus licheniformis |
| CN109468249A (en) * | 2018-12-26 | 2019-03-15 | 佛山市艳晖生物科技有限公司 | A kind of microbial bacterial agent and its application on livestock and poultry cultivation |
| CN110283752A (en) * | 2019-07-11 | 2019-09-27 | 中国科学院青岛生物能源与过程研究所 | The production method and product of the culture medium and production method of bacillus licheniformis, bacillus licheniformis opportunistic pathogen powder |
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