CN115957371A - Gel application for repairing skin injury and preparation method and application thereof - Google Patents
Gel application for repairing skin injury and preparation method and application thereof Download PDFInfo
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- CN115957371A CN115957371A CN202211479579.5A CN202211479579A CN115957371A CN 115957371 A CN115957371 A CN 115957371A CN 202211479579 A CN202211479579 A CN 202211479579A CN 115957371 A CN115957371 A CN 115957371A
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- solution
- hyaluronic acid
- skin
- free radical
- gel
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 43
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- 239000003431 cross linking reagent Substances 0.000 claims abstract description 7
- 239000000835 fiber Substances 0.000 claims abstract description 5
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- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims description 6
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the field of skin repair, and particularly relates to a skin injury repair gel patch as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) Preparing alginate aqueous solution (2), adding free radical scavenging molecules into hyaluronic acid aqueous solution; (3) Adding the hyaluronic acid-coated free radical scavenging molecules into an alginate aqueous solution, and adding a cross-linking agent for cross-linking; pouring into a mould or loading on gauze or other fibre to form a skin injury repairing gel application. The composition of alginate is adjusted by adding anti-inflammatory molecules with amino groups, and the hydrophobic free radical scavenging factors are wrapped in hyaluronic acid solution which can be absorbed by the skin in a penetrating way, so that the hydrophobic free radical scavenging factors are quickly released. Meanwhile, the hydrogel can also block the invasion of external bacteria, so that skin ulceration caused by bacterial infection on the wound surface is avoided.
Description
Technical Field
The invention belongs to the field of skin repair, and particularly relates to a skin injury repair gel application as well as a preparation method and application thereof.
Background
The hydrogel is a porous material with a three-dimensional network structure, has good hydrophilicity, can not be dissolved, integrates the properties of moisture retention, water retention and the like, and is a material commonly used in tissue engineering repair. Alginate is a natural polysaccharide, has good biocompatibility and is widely applied in the field of tissue engineering. Negatively charged alginates, however, cause immunological rejection effects when in contact with the body. Most of free radical scavenging factors are hydrophobic drugs, and the drugs are difficult to be maintained on the wound surface for a long time after being directly smeared, and a large amount of drugs have potential risk of cytotoxicity on the damaged part. Therefore, how to realize the slow release of the hydrophobic drug in the hydrogel is an urgent problem to be solved, and the hydrogel can remove free radicals and promote angiogenesis at the injured part.
With the continuous progress of science and technology, nuclear technology is increasingly used in daily life and production. While nuclear technology brings convenience, exposure to nuclear radiation by accident or improper operation also brings certain damage to people's daily life. Not only nuclear radiation and radiotherapy, but also skin damage caused by solar ultraviolet radiation in daily life requires care and treatment. The ionizing radiation is mainly caused by the fact that after an organism is irradiated, the form and the structure of biomacromolecules, proteins and the like of the organism are changed due to energy transfer. Because the human body contains more than 70 percent of water, active oxygen and free radicals generated after the human body is subjected to ionizing radiation are main causes of radiation damage, and the health of the human body is seriously influenced. Unlike common skin lesions (such as cuts, contusions, full-thickness skin lesions), radiation-damaged skin does not have significant damage, but deep-layer cellular DNA of skin is broken by radiation energy, and the broken DNA forms abnormal cells during self-replication. Meanwhile, a large amount of free radicals are generated in the radiation process, so that cells die due to oxidative stress, and subcutaneous vascular malformation is the main reason that the radiation-damaged skin is difficult to repair. Therefore, rapid scavenging of free radicals while promoting revascularization at the site of injury is important to promote radiation damage to the skin. However, most of the current clinical treatment methods for radiation-induced injuries are surgical debridement, hyperbaric oxygen treatment and the like, and are complex to operate and expensive.
Disclosure of Invention
The invention aims to provide a skin injury repairing gel application and a preparation method and application thereof.
In order to realize the purpose, the invention adopts the technical scheme that:
a preparation method of a gel patch for repairing skin injury comprises the following steps:
(1) Preparing alginate water solution, stirring overnight, and swelling;
(2) Adding the free radical scavenging molecules into a hyaluronic acid aqueous solution, stirring at room temperature, centrifuging and removing redundant hyaluronic acid to obtain free radical scavenging molecules coated with hyaluronic acid;
(3) Adding the hyaluronic acid-coated free radical scavenging molecules obtained in the step (2) into the alginate aqueous solution obtained in the step (1), and fully and uniformly mixing to obtain a mixed solution;
(4) Adding a cross-linking agent into the mixed solution obtained in the step (3) for cross-linking;
(5) And (3) pouring the mixed solution added with the cross-linking agent in the step (4) into a mould or loaded on gauze or other fibers to form a skin injury repairing gel application.
The free radical scavenging molecules are one or more of astaxanthin, resveratrol, curcumin, gallic acid, ferulic acid and dopamine, and the concentration of the free radical scavenging molecules is 0.005-0.02 (g/ml) of the volume of the hyaluronic acid solution.
The alginate has a mass volume concentration of 0.01-0.02 (g/ml) and a molecular weight of 10-500 kDa.
The mass volume concentration of the hyaluronic acid aqueous solution is 0.01-0.02 (g/ml), and the molecular weight is 2kDa-500kDa.
The cross-linking agent is one or a mixture of two of polymer molecules with amino and divalent metal ions.
The polymer molecule with amino is one or a mixture of more of quaternized chitosan, chitosan oligosaccharide and L-arginine.
The divalent metal ion is one of calcium, magnesium, copper and barium.
Preferably, the preparation method of the gel dressing for repairing skin injury comprises the following steps:
(1) Weighing sodium alginate powder, swelling with deionized water overnight to obtain uniform and transparent sodium alginate water solution with concentration of 0.01 g/ml;
(2) Weighing hyaluronic acid powder, and swelling in deionized water overnight to obtain hyaluronic acid solution with concentration of 0.01 g/ml;
(3) Adding the free radical scavenging factor into the hyaluronic acid solution, stirring at high speed until the solution is uniformly formed into (7.5-10)/0.5 (mg/ml) of free radical scavenging factor sodium hyaluronate solution, centrifuging the solution, and adding the solution into a mixing tank at a ratio of 1:1-2, and stirring at high speed to form a uniform sodium alginate-hyaluronic acid solution mixed solution;
(4) Adding a quaternary ammoniated chitosan solution with the mass fraction of 1% and a divalent metal ion solution with the mass fraction of 10% into a mixed solution of a alginic acid solution and a hyaluronic acid solution, and realizing molecular crosslinking through electrostatic interaction; wherein the volume ratio of the quaternary ammoniated chitosan solution to the divalent metal ion solution is 1:5;
(5) And (5) pouring the mixed solution of the quaternary ammoniation chitosan solution and the divalent metal ion solution added in the step (4) into a mould or loaded on gauze or other fibers to form the skin injury repairing gel with the functions of removing free radicals and resisting bacteria.
The invention also comprises a skin injury repairing gel application prepared by the preparation method.
The invention also comprises application of the skin injury repairing gel patch in preparation of a patch for skin injury.
Compared with the prior art, the invention has the beneficial effects that:
the composition of alginate is adjusted by adding anti-inflammatory molecules with amino groups, and the hydrophobic free radical scavenging factors are wrapped in hyaluronic acid solution which can be absorbed by a permeable skin, so that the hydrophobic free radical scavenging factors are quickly released. The hydrogel material based on radiation damage can provide a moist environment for the damaged part, has double functions of eliminating free radicals caused by radiation and promoting angiogenesis by medicaments, and is particularly suitable for delaying the occurrence of radiation damage; meanwhile, the hydrogel can also block the invasion of external bacteria, so that skin ulceration caused by bacterial infection on the wound surface is avoided.
Meanwhile, the matrix materials sodium alginate and hyaluronic acid used in the invention are natural polymers, have good biocompatibility, mild condition in the preparation process, simple operation, no by-product and no toxicity, and do not need to add a catalyst; the gel plaster for repairing skin injury provided by the invention has certain effects of preventing and treating skin injury caused by solar ultraviolet radiation, skin radiation injury caused by nuclear radiation or nuclear contamination and skin injury of radiotherapy patients.
Drawings
FIG. 1 is a topographical view of different sized skin lesion repair gel patches prepared in examples 1-3 of the present invention.
FIG. 2 is a graph showing the water content of skin lesion repair gel patches prepared in examples 1-3 of the present invention.
FIG. 3 is a graph showing the results of biocompatibility characterization of skin lesion repair gel patches prepared in examples 1-3 of the present invention. FIG. 4 is a graph showing the results of characterizing the radical scavenging effect of the skin lesion repairing gel patch prepared in examples 1 to 3 of the present invention.
FIG. 5 is a graph showing the effect of example 3 of the present invention on the local radiation damage repair of mice.
FIG. 6 is a diagram of the angiogenesis after the local radiation damage repair of the mouse in the embodiment 3 of the invention.
FIG. 7 is a graph showing the cumulative release of example 3 of the present invention and comparative example 1.
FIG. 8 shows the cytotoxicity characterization results of example 3 and astaxanthin.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings and preferred embodiments.
Example 1: the preparation method of the skin injury repairing gel comprises the following steps: weighing 0.1g of sodium alginate (molecular weight is 10-500 kDa, the same below) powder, and swelling with 10ml of deionized water overnight to obtain uniform and transparent sodium alginate solution; meanwhile, 0.1g of hyaluronic acid (molecular weight of 2kDa-500kDa, the same applies below) powder was weighed and swollen overnight in 10ml of deionized water to obtain a hyaluronic acid solution with a concentration of 1%. Then, adding astaxanthin (7.5 mg) into 0.5ml of hyaluronic acid solution, stirring at high speed until the solution is uniform, centrifuging, adding the astaxanthin-hyaluronic acid solution into 1ml of sodium alginate solution, stirring at high speed to form uniform solution, and standing at room temperature for defoaming for 2 hours. Finally, a 10% calcium chloride solution (0.5 ml) was slowly added to the mixed solution along the edge of the mold, and after 4 hours of crosslinking, a skin injury repair gel containing a radical removal factor was obtained, and it was shown that the addition of divalent metal ions enabled the obtained skin injury repair gel to have a proper strength, as shown in a of fig. 1.
Example 2: the preparation method of the skin injury repairing gel comprises the following steps: weighing 0.1g of sodium alginate powder, and swelling with 10ml of deionized water overnight to obtain a uniform and transparent sodium alginate solution; meanwhile, 0.1g of hyaluronic acid powder was weighed and swollen overnight in 10ml of deionized water to obtain a hyaluronic acid solution with a concentration of 1%. Adding the chenopodium album alcohol (10 mg) into 0.5ml of hyaluronic acid solution, stirring at high speed until the solution is uniform, adding the chenopodium album alcohol-hyaluronic acid solution into 1ml of sodium alginate solution after centrifugation, stirring at high speed to form uniform mixed solution, finally adding quaternary ammoniated chitosan solution (100 mu l) with the mass fraction of 1% into the mixed solution of the alginic acid solution and the hyaluronic acid solution, and realizing molecular crosslinking of the skin injury repair gel containing the free radical removal factor and the antibacterial function through electrostatic interaction. The results show that although sodium alginate can be crosslinked by adding quaternary ammoniated chitosan solution only, and the product has an antibacterial function due to the chitosan, the crosslinking strength is not enough, so that the product is not suitable for being used as gel or plaster, and is shown as b in figure 1.
Example 3: the preparation method of the skin injury repairing plaster comprises the following steps: weighing 0.1g of sodium alginate powder, and swelling with 10ml of deionized water overnight to obtain a uniform and transparent sodium alginate solution; meanwhile, 0.1g of hyaluronic acid powder was weighed and swollen overnight in 5ml of deionized water to obtain a hyaluronic acid solution with a concentration of 2%. Adding free radical scavenging molecule ferulic acid (10 mg) into 0.5ml hyaluronic acid solution, stirring at high speed to be uniform, then adding ferulic acid-hyaluronic acid solution into 1ml sodium alginate solution, stirring at high speed to form uniform solution, finally adding quaternary ammonified chitosan solution (100 mu l) and 10% calcium chloride solution (0.5 ml) with the mass fraction of 1% into the mixed solution, dripping the solution onto gauze with the proper wound size, and crosslinking to obtain the skin injury repair patch with free radical removal factor and high strength of antibacterial activity.
Comparative example 1: the preparation method of the skin injury repair patch comprises the following steps: weighing 0.1g of sodium alginate powder, and swelling with 10ml of deionized water overnight to obtain a uniform and transparent sodium alginate solution; adding astaxanthin (10 mg) as free radical scavenging molecule into 0.5ml sodium alginate solution, stirring at high speed to uniformity, dripping onto gauze with appropriate wound size, adding 1% quaternary ammonification chitosan solution (100 μ l) and calcium chloride solution (0.5 ml) for crosslinking to obtain skin injury repair patch containing no hyaluronic acid and having free radical removal factor and antibacterial activity
And (3) testing: 1. calculating the water content of the skin injury repair application: first, skin injury repair patches of the above examples 1 to 3 were prepared. The prepared gel was placed in 0.9% physiological saline to remove unreacted small molecules. The surface moisture was then blotted dry and weighed to obtain the wet weight of the gel as M wet, FIG. 1 is a topographical map of skin lesion repair gel patches of different sizes and strengths prepared in examples 1-3 of the present invention. Subsequently, the gel was placed in an oven at 60 ℃ and dried for 3 days, and the dried gel was weighed Mdry. The operation is repeated five times, and the average water content is calculated according to the formula (1). As shown in FIG. 2, the water content of the gel was 98% or more. Formula (1) water content (%) = (M wet-M dry)/M wet × 100%.
2. Skin injury repair patch biocompatibility assessment: the biocompatibility of the skin injury gel dressing was evaluated primarily by toxicity testing of the gel application against cells. The 3- (4,5-dimethylthiazole-2) -2,5-diphenyltetrazolium bromide (MTT) assay is the most commonly used method for cytotoxicity assay evaluation. Firstly, the methodSkin injury gels containing free radical scavenging molecules were prepared and sterilized overnight under uv irradiation. The prepared gel was then soaked in DMEM complete medium containing FBS and penicillin streptomycin and incubated at 37 ℃ for 24h to leach out soluble impurities from the gel. The mouse-derived fibroblast NIH-3T3 cells (1X 105 cells/ml) were seeded in a 96-well plate at 37 ℃ with 5% CO 2 And culturing for 24 hours in an incubator. After the cells are spread adherent to the wall, the culture solution is sucked out, the gel leaching liquor is added, and the fresh culture medium group is used as a control group. After 2 days of incubation in a CO2 incubator, MTT reagent was added, after 4 hours of incubation in the dark, the solution was blotted dry, 200. Mu.l DMSO solution was added, and shaking was performed on a shaker in the dark for 10min to dissolve the precipitate completely. Finally, 100. Mu.l of the solution was pipetted into a new 96-well plate and the absorbance of the solution at 570nm was measured using a microplate reader. The toxicity of the hydrogel sample to the cells is calculated by a formula 2, and 5 compound holes are formed in the sample and the control group so as to ensure that the data have statistical significance. FIG. 3 is a graph showing the results of biocompatibility characterization of a skin lesion repair gel patch. As can be seen from FIG. 3, the patch samples prepared were substantially free of cytotoxicity, with a cell survival rate of greater than 92%. Equation 2: cytotoxicity (%) = (sample absorbance-blank absorbance)/(control absorbance-blank absorbance) × 100%.
3. Evaluation of free radical scavenging effect of skin injury repair patch the evaluation of antioxidant effect of the radiation injury repair patch was mainly detected by 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) method. First, a skin injury repair gel was prepared as described in example 2. The free radical scavenging rate of the gel material was calculated by placing the applied sample (5 mM diameter gel piece) in a DPPH ethanol solution having a concentration of 0.1mM, observing the time for the solution to change from purple to yellow, and measuring the absorbance of the solution for 30min, 1h and 2h. FIG. 4 is a graph showing the results of characterizing the radical scavenging effect of the skin lesion repairing gel patch prepared in examples 1 to 3 of the present invention. As can be seen from a in FIG. 4, the clearance of the gel at 2h is similar to the radical scavenging effect of the simple radical scavenger, and the gel changes from purple to yellow. In FIG. 4, b is the radical clearance of the gel material calculated by the absorbance of 1h, and the result shows that the clearance of the gel reaches more than 80% at 1 h.
3. Local radiation skin injury model repair of mice construction of local radiation injury model, after anesthesia of mice, the hindlimb skin was exposed to 40Gy of X-rays and irradiated at a dose rate of 2Gy/min for 20 minutes (Biological X-ray irradiation, rad Source, RS 2000). After irradiation, the mice at the irradiated sites were observed daily and kept in good environment for 10 days. When the mouse hind limb irradiation site had depilation, red swelling, etc., it was fixed to the injury site with a prepared circular hydrogel patch (diameter 8 mm) and applied to a commercial wound dressing (see below)
) Controls were treated. The hydrogel dressing was changed every two days. Wound recovery was observed and photographed at days 0, 7, and 14 after treatment. As seen from FIGS. 5 and 6, the control group had developed ulcerated bleeding after 7 days, while the gel patch treatment did not produce regression of redness and swelling of the injured part, resulting in regeneration of hair. Tissue section staining of the treated skin revealed no neovascularization in the control skin tissue by CD31 staining, whereas tissue section staining of mice treated with gel application revealed massive neovascularization after 14 days.
5. Cumulative release of free radical scavenger before and after Hyaluronic Acid (HA) coating: skin lesion gels of example 3 and comparative example 1 were prepared. The applied sample (5 mm diameter gel piece) was placed in 5ml PBS, placed on a 37 ℃ water bath shaker, the solution removed at the specified time, and an equal amount of fresh PBS was added. And finally, carrying out quantitative analysis on the free radical scavenging factors in the solution by high performance liquid chromatography. FIG. 7 is a graph showing the cumulative release of example 3 of the present invention and comparative example 1. From FIG. 7, it can be seen that the radical scavenger improves its hydrophobic property after coating HA, and facilitates its release rate in the gel.
6. Cytotoxicity test of free radical scavenger before and after Hyaluronic Acid (HA) coating: the hyaluronic acid-astaxanthin and an equal amount of pure astaxanthin molecules from example 1 were subjected to cytotoxicity assay as assay 2. As can be seen in FIG. 8, the cytotoxicity of the free radical scavenging molecule was significantly reduced after the hyaluronic acid coating.
It can be seen from the above examples 1-3 and comparative examples that the skin injury repair gel has good mechanical strength and biocompatibility after cross-linking, and the introduction of hyaluronic acid not only makes up the release performance of hydrophobic free radical scavenging molecules in the skin injury repair gel, but also enhances the functional defects of alginate gel dressing in terms of free radical scavenging and antibacterial property, and can effectively inhibit the level of free radicals in cells, and promote angiogenesis, thereby promoting skin injury repair.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A preparation method of a gel patch for repairing skin injury is characterized by comprising the following steps:
(1) Preparing alginate water solution, stirring overnight, and swelling;
(2) Adding the free radical scavenging molecules into a hyaluronic acid aqueous solution, stirring at room temperature, centrifuging and removing redundant hyaluronic acid to obtain free radical scavenging molecules coated with hyaluronic acid; ,
(3) Adding the hyaluronic acid-coated free radical scavenging molecules obtained in the step (2) into the alginate aqueous solution obtained in the step (1), and fully and uniformly mixing to obtain a mixed solution;
(4) Adding a cross-linking agent into the mixed solution obtained in the step (3) for cross-linking;
(5) And (5) pouring the mixed solution added with the cross-linking agent in the step (4) into a mould or loaded on gauze or other fibers to form a skin injury repairing gel application.
2. The method of preparing a skin lesion repairing gel patch according to claim 1, wherein said free radical scavenger molecules are one or more of astaxanthin, resveratrol, curcumin, gallic acid, ferulic acid, and dopamine, and the amount of free radical scavenger molecules added is 0.005-0.02 (g/ml) of the volume of hyaluronic acid solution.
3. The method for preparing a gel patch for repairing skin lesions according to claim 1, wherein the alginate has a mass volume concentration of 0.01-0.02 (g/ml) and a molecular weight of 10kDa-500kDa.
4. The method of preparing a skin lesion repairing gel patch according to claim 1, wherein said aqueous solution of hyaluronic acid has a mass volume concentration of 0.01-0.02 (g/ml) and a molecular weight of 2kDa-500kDa.
5. The method for preparing a gel patch for repairing skin damage according to claim 1, wherein said cross-linking agent is one or a mixture of two of polymer molecules with amino groups and divalent metal ions.
6. The method for preparing a gel patch for repairing skin injury according to claim 5, wherein the polymer molecule with amino group is one or more of quaternized chitosan, chitosan oligosaccharide, and L-arginine.
7. The method for preparing a gel patch for repairing skin injury according to claim 5, wherein the divalent metal ion is one of calcium, magnesium, copper and barium.
8. The method for preparing a skin lesion repairing gel patch according to claim 1, wherein the following steps are adopted:
(1) Weighing sodium alginate powder, swelling with deionized water overnight to obtain a uniform and transparent sodium alginate solution water solution with the concentration of 0.01 g/ml;
(2) Weighing hyaluronic acid powder, and swelling in deionized water overnight to obtain hyaluronic acid solution with concentration of 0.01 g/ml;
(3) Adding the free radical scavenging factor into the hyaluronic acid solution, stirring at high speed until the solution is uniformly formed into (7.5-10)/0.5 (mg/ml) of free radical scavenging factor sodium hyaluronate solution, centrifuging the solution, and adding the solution into a mixing tank at a ratio of 1:1-2, and stirring at high speed to form a uniform sodium alginate-hyaluronic acid solution mixed solution;
(4) Adding a quaternary ammoniation chitosan solution with the mass fraction of 1% and a divalent metal ion solution with the mass fraction of 10% into a mixed solution of a sodium alginate solution and a hyaluronic acid solution, and realizing molecular crosslinking through electrostatic interaction; wherein the volume ratio of the quaternary ammoniated chitosan solution to the divalent metal ion solution is 1:5;
(5) And (3) pouring the mixed solution of the quaternary ammoniated chitosan solution and the divalent metal ion solution added in the step (4) into a mould or loaded on gauze or other fibers to form the radiation damage repairing gel containing the antioxidant and the antibacterial functions.
9. A skin injury repair gel patch obtained by the method of manufacture of any one of claims 1 to 8.
10. Use of a skin lesion repair gel dressing according to claim 9 for preparing a dressing for skin lesions.
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