CN115931855A - Sample reading device and sample reading method - Google Patents
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- CN115931855A CN115931855A CN202111114017.6A CN202111114017A CN115931855A CN 115931855 A CN115931855 A CN 115931855A CN 202111114017 A CN202111114017 A CN 202111114017A CN 115931855 A CN115931855 A CN 115931855A
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000003384 imaging method Methods 0.000 claims abstract description 292
- 239000000523 sample Substances 0.000 claims description 102
- 230000003287 optical effect Effects 0.000 claims description 12
- 239000012472 biological sample Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000013077 scoring method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 description 7
- 230000004075 alteration Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
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Abstract
The invention discloses a sample slide viewing device and a sample slide viewing method.A multiple imaging device at least comprises a low-power light path consisting of an objective lens group, a first ocular and first imaging acquisition equipment, a high-power light path consisting of the objective lens group, a second ocular and second imaging acquisition equipment, and the objective lens group consisting of an objective lens and a light splitting device; the imaging information of the sample is respectively generated into the same initial imaging information in a first direction and a second direction through a light splitting device; the method comprises the steps that first imaging information of initial imaging information in a first direction after passing through a first eyepiece is obtained by first imaging obtaining equipment; the second imaging obtaining equipment obtains second imaging information of the initial imaging information in the second direction after the initial imaging information passes through a second eyepiece; the multiple of the second imaging information is higher than the multiple of the first imaging information; a sample movement control device; the processing device is used for processing first image information formed by the first imaging acquisition equipment and second image information formed by the second imaging acquisition equipment. The efficiency is improved, and the vibration is avoided.
Description
Technical Field
The invention relates to the technical field of imaging equipment, in particular to a sample reading device and a sample reading method.
Background
In the traditional clinical examination, the sample reading device is widely applied.
Taking the example that the sample is a blood smear, the blood smear is usually analyzed by a human inspection by an optical microscope, and the technical and experience requirements of the inspection physician are extremely high and time-consuming and labor-consuming. The current slide reading device automatically shoots characteristic cells in a blood slide through a microscopic imaging system.
At present, a film reading device is designed with a group of optical amplification light paths, an imaging camera, a set of sample movement control device and a processor. Generally, the region of interest is determined during the photographing process, and then the target cell is photographed. In order to determine the region of interest and the target cell, the amplifying optical path is usually designed with a plurality of objective lenses. The region of interest is determined by the low power objective lens, and then the high power objective lens is switched to take a picture of a single cell (target cell).
Due to the fact that the high-power objective lens and the low-power objective lens need to be switched, the efficiency of the film reading device is reduced, and the efficiency of sample analysis of an instrument is also reduced; moreover, the requirement of high power imaging on vibration suppression of the whole system is extremely high, and vibration is inevitably generated by switching the high power objective lens and the low power objective lens, so that the whole imaging system is negatively influenced.
Therefore, how to improve the efficiency and avoid the vibration caused by switching the objective lens is a problem to be solved urgently by those skilled in the art.
Disclosure of Invention
In view of the above, the present invention provides a specimen reading device to improve efficiency and avoid switching objective lens to generate vibration. The invention also provides a sample reading method.
In order to achieve the purpose, the invention provides the following technical scheme:
a specimen interpreting apparatus, comprising:
the multi-time imaging device at least comprises a low-time light path and a high-time light path, and comprises an objective lens, a light splitting device, a first ocular lens, a second ocular lens, a first imaging acquisition device and a second imaging acquisition device; after the imaging information of the sample passes through the objective lens, the same initial imaging information is generated in the first direction and the second direction respectively under the action of the light splitting device; the objective lens and the light splitting device form an objective lens group; the low power light path is composed of the objective lens group, the first ocular lens and the first imaging acquisition equipment, and the first imaging acquisition equipment acquires first imaging information of the initial imaging information in the first direction after passing through the first ocular lens; the high power light path is composed of the objective lens group, the second ocular lens and the second imaging acquisition equipment, and the second imaging acquisition equipment acquires second imaging information of the initial imaging information in the second direction after passing through the second ocular lens; a multiple of the second imaging information is higher than a multiple of the first imaging information;
sample movement control means for regulating movement of the sample relative to the objective lens;
and the processing device is used for processing first image information formed after the first imaging acquisition equipment acquires the first imaging information and second image information formed after the second imaging acquisition equipment acquires the second imaging information.
The invention also provides a sample reading method, which comprises the following steps:
the imaging information of the sample passes through the objective lens and then generates the same initial imaging information in the first direction and the second direction respectively; acquiring first imaging information of the initial imaging information in the first direction after passing through the first eyepiece, and acquiring second imaging information of the initial imaging information in the second direction after passing through the second eyepiece; a multiple of the second imaging information is higher than a multiple of the first imaging information;
adjusting the specimen to move relative to the objective lens;
and processing first image information formed after the first imaging information is obtained and second image information formed after the second imaging information is obtained.
According to the technical scheme, the objective lens, the light splitting device, the first eyepiece and the first imaging acquisition equipment of the multi-time imaging device form a low-power light path, and the objective lens, the light splitting device, the second eyepiece and the second imaging acquisition equipment form a high-power light path, wherein the multiple of the second imaging information acquired by the second imaging acquisition equipment is higher than the multiple of the first imaging information acquired by the first imaging acquisition equipment, the first image information and the second imaging information with different multiples can be acquired through the adjustment, the processing device processes the first image information formed after the first imaging information is acquired by the first imaging acquisition equipment, and processes the second image information formed after the second imaging information is acquired by the second imaging acquisition equipment, so that the image reading operation is realized. Through the arrangement, the switching operation of the high-power objective lens and the low-power objective lens is effectively avoided, and the efficiency of the specimen reading device is ensured. And moreover, vibration caused in the process of switching the objective lens is avoided, the imaging effect is ensured, and negative influence on the whole imaging process is avoided. The efficiency of the sample slide reading device is improved, vibration caused by switching of the objective lens is avoided, and negative influence on the whole imaging process is avoided.
The sample reading method provided by the invention has the same technical effects as the sample reading device, and the description is not repeated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a first structural schematic diagram of a specimen interpreting device according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a second specimen interpreting device according to an embodiment of the present invention.
Detailed Description
The invention discloses a sample reading device, which is used for improving the efficiency and avoiding the vibration caused by switching an objective lens. The invention also provides a sample reading method.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 and fig. 2, an embodiment of the present invention provides a specimen interpreting device, which includes a multiple imaging device, a specimen movement control device, and a processing device. The multiple imaging device at least comprises a low-power light path and a high-power light path, and comprises an objective lens 1, a light splitting device 2, a first ocular 3, a second ocular 6, a first imaging acquisition device 4 and a second imaging acquisition device 7; after the imaging information of the sample passes through the objective lens 1, the same initial imaging information is generated in the first direction and the second direction respectively under the action of the light splitting device 2; the objective lens 1 and the light splitting device 2 form an objective lens group; the low power optical path is composed of an objective lens group, a first ocular lens 3 and a first imaging acquisition device 4, wherein the first imaging acquisition device 4 acquires first imaging information of initial imaging information in a first direction after the initial imaging information passes through the first ocular lens 3; the high-power optical path consists of an objective lens group, a second ocular 6 and a second imaging acquisition device 7, and the second imaging acquisition device 7 acquires second imaging information of initial imaging information in a second direction after the initial imaging information passes through the second ocular 6; the multiple of the second imaging information is higher than the multiple of the first imaging information; the sample movement control device is used for adjusting the movement of the sample relative to the objective lens 1; the processing device is used for processing first image information formed after the first imaging acquisition equipment 4 acquires the first imaging information and second image information formed after the second imaging acquisition equipment 7 acquires the second imaging information.
According to the sample reading device provided by the embodiment of the invention, a low-power optical path is formed by the objective lens 1, the light splitting device 2, the first eyepiece 3 and the first imaging acquisition equipment 4 of the multiple imaging device, and a high-power optical path is formed by the objective lens 1, the light splitting device 2, the second eyepiece 6 and the second imaging acquisition equipment 7, wherein the multiple of the second imaging information acquired by the second imaging acquisition equipment 7 is higher than the multiple of the first imaging information acquired by the first imaging acquisition equipment 4, through the adjustment, the first image information and the second imaging information with different multiples can be acquired, the processing device processes the first image information formed after the first imaging information is acquired by the first imaging acquisition equipment 4, and processes the second image information formed after the second imaging information is acquired by the second imaging acquisition equipment 7, so that the reading operation is realized. Through the arrangement, the switching operation of the high-power objective lens and the low-power objective lens is effectively avoided, and the efficiency of the specimen slide reading device is ensured. And moreover, vibration caused in the process of switching the objective lens is avoided, the imaging effect is ensured, and negative influence on the whole imaging process is avoided. The efficiency of the specimen slide reading device is improved, vibration caused by switching of the objective lens is avoided, and negative influence on the whole imaging process is avoided.
In the specific operation process, a sample is adjusted to move relative to the object lens 1 by using the sample moving control device, and the imaging information of the sample passes through the object lens 1 and then generates the same initial imaging information in a first direction and a second direction respectively; acquiring first imaging information of the initial imaging information in the first direction after passing through a first ocular 3, and acquiring second imaging information of the initial imaging information in the second direction after passing through a second ocular 6; the second imaging information is higher in multiple than the first imaging information.
Preferably, the movement of the specimen by the specimen movement control means may depend on the first imaging information acquired by the first imaging acquisition apparatus 4. The operator uses the sample movement control device to adjust the sample to move relative to the objective lens 1 according to at least the first imaging information acquired by the first imaging acquisition device 4, and determines the region of interest of the sample. That is, it is determined whether the position of the specimen with respect to the objective lens 1 is the region of interest of the specimen by the first imaging information acquired by the first imaging acquisition apparatus 4; if the position of the sample relative to the objective lens 1 is determined to be the region of interest of the sample, the first imaging information acquired by the first imaging acquisition device 4 can be processed by the processing means to form first image information, and the second imaging information acquired by the second imaging acquisition device 7 at the moment can be processed by the processing means to form second image information; if the position of the sample relative to the objective lens 1 is determined not to be the region of interest of the sample, the sample movement control device is required to adjust the movement of the sample relative to the objective lens 1 until the position of the sample relative to the objective lens 1 is determined to be the region of interest of the sample through the first imaging information acquired by the first imaging acquisition device 4, in this state, the first imaging information acquired by the first imaging acquisition device 4 is processed by the processing device to form first image information, and the second imaging information acquired by the second imaging acquisition device 7 at this time is processed by the processing device to form second image information.
Therefore, the basis of the movement of the sample relative to the objective lens 1 by the sample movement control device is the first imaging information acquired by the first imaging acquisition device 4, so that the sample movement control device can be in communication connection with the first imaging acquisition device 4, and the operator can control the sample movement control device according to the first imaging information acquired by the first imaging acquisition device 4, so that the sample movement control device adjusts the movement of the sample relative to the objective lens 1.
Of course, the sample movement control means may also be moved in accordance with the second imaging information acquired by the second imaging acquisition apparatus 7. However, since the second imaging information acquired by the second imaging acquisition device 7 should fall into the region of interest of the sample, and since the multiple of the second imaging information is higher than the multiple of the first imaging information, the difficulty of determining the region of interest of the sample by the second imaging information acquired by the second imaging acquisition device 7 is greater than the difficulty of determining the region of interest of the sample by the first imaging information acquired by the first imaging acquisition device 4.
The region of interest of the sample is a suitable region for the sample in the operation process of the sample reading device, and the suitable region of the sample is determined according to the type of the sample and the requirement of the sample reading operation, which are not described in detail one by one and are all within the protection range.
In a first embodiment, as shown in fig. 1, the objective lens group in the low power optical path is a low power objective lens group, and the objective lens group in the high power optical path is a high power objective lens group. The high power objective lens group further comprises a magnifying lens group 5, and initial imaging information generated in the second direction under the action of the light splitting device 2 passes through the magnifying lens group 5 and then passes through a second ocular lens 6 to form second imaging information. By adding the magnifying lens group 5, the imaging magnification of the second imaging information is increased. In the present embodiment, the magnifications of the first eyepiece 3 and the second eyepiece 6 may be the same or different. It is necessary to ensure that the multiple of the second imaging information obtained by the second imaging obtaining device 7 after the initial imaging information in the second direction passes through the magnifying lens group 5 and the second ocular lens 6 is greater than the multiple of the first imaging information obtained by the first imaging obtaining device 4 after the initial imaging information in the first direction passes through the first ocular lens 3.
In a second embodiment, as shown in fig. 2, the magnification of the first eyepiece 3 is lower than that of the second eyepiece 6. With the arrangement, the magnification of the second imaging information can be higher than that of the first imaging information only through the difference between the first eyepiece 3 and the second eyepiece 6, without adding a magnifying component (such as a magnifying lens group 5) and the structure of the sample reading device is effectively simplified.
In order to improve the general degree of the specimen slide reading device, at least one of the first ocular 3 and the second ocular 6 is a replaceable ocular. Preferably, only the second eyepiece 6 is a replaceable eyepiece and the first eyepiece 3 is a non-replaceable eyepiece. And according to the type of the sample, the film reading requirement and other conditions, the multiple between the first ocular 3 and the second ocular 6 meets the requirement.
Preferably, the first imaging acquisition device 4 and the second imaging acquisition device 7 are cameras; the first image information and the second image information are pictures formed after the camera shoots. The first imaging acquisition apparatus 4 and the second imaging acquisition apparatus 7 may be other apparatuses, such as a drawing apparatus or an imaging apparatus, and the like, without being limited thereto.
In order to improve the compactness and ensure the light splitting effect, the light splitting device 2 is a light splitter. The spectroscopic device 2 may be a component such as a one-to-many optical fiber bundle.
The beam splitter is preferably an achromatic beam splitter. The achromatic spectroscope corrects the chromatic aberration of light of a plurality of wavelengths (such as blue light, green light, red light, and the like), so that the chromatic aberration can be reduced or eliminated, and the imaging effect of the first imaging acquisition device 4 and the second imaging acquisition device 7 can be improved.
In addition, the first eyepiece 3 and the second eyepiece 6 may also adopt an achromatic lens, and the achromatic lens corrects chromatic aberration of light with multiple wavelengths (such as blue light, green light, red light, and the like), so as to further reduce or eliminate chromatic aberration, thereby further improving the imaging effect of the first imaging acquisition device 4 and the second imaging acquisition device 7.
In the specimen interpreting apparatus provided in this embodiment, the objective lens 1 is an objective lens with a magnification, which further improves the imaging magnification. It is also possible to make the objective lens 1 an objective lens without magnification.
To simplify the structure, the first eyepiece 3 and the first image capturing apparatus 4 are located on the imaging path in the first direction. With the arrangement, initial imaging information generated in the first direction after the imaging information of the sample passes through the objective lens 1 can directly pass through the first eyepiece 3 and be acquired by the first imaging acquisition device 4, and a component (such as a reflector and the like) for adjusting the direction is avoided.
As above, the second eyepiece 6 and the second image capturing apparatus 7 are located on the imaging path in the second direction. With the above arrangement, initial imaging information generated in the second direction after the imaging information of the sample passes through the objective lens 1 can directly pass through the second eyepiece 6 (and the magnifying lens group 5) and be acquired by the second imaging acquisition device 7, and components (such as a reflector and the like) for adjusting the direction are avoided.
In this example, the sample is a smear of biological sample.
The biological sample smear can be a blood smear, a spinal fluid smear or a stool smear, etc.
The invention also provides a sample reading method, which comprises the following steps:
the imaging information of the sample passes through the objective lens 1 and then generates the same initial imaging information in the first direction and the second direction respectively; acquiring first imaging information of the initial imaging information in the first direction after passing through a first ocular 3, and acquiring second imaging information of the initial imaging information in the second direction after passing through a second ocular 6; the multiple of the second imaging information is higher than the multiple of the first imaging information;
adjusting the sample to move relative to the objective lens 1;
and processing first image information formed after the first imaging information is acquired and second image information formed after the second imaging information is acquired.
The invention also provides a sample reading method, wherein the imaging information of the sample passes through the objective lens 1 and then respectively generates the same initial imaging information in the first direction and the second direction, and the multiple of the second imaging information formed after respectively passing through the first ocular lens 3 and the second ocular lens 6 is higher than the multiple of the first imaging information. By adjusting the movement of the specimen relative to the objective lens 1, the region of interest of the specimen can be determined. After the region of interest of the sample can be observed through the first imaging information and/or the second imaging information, and the region of interest of the sample is determined, the first image information formed after the first imaging information is acquired and the second image information formed after the second imaging information is acquired are processed, so that the film reading operation is realized. Through the arrangement, the switching operation of the high-power objective lens and the low-power objective lens is effectively avoided, and the efficiency of the specimen reading device is ensured. And moreover, vibration caused in the process of switching the objective lens is avoided, the imaging effect is ensured, and negative influence on the whole imaging process is avoided.
Further, the movement of the sample relative to the objective lens 1 is adjusted at least according to the first imaging information acquired by the first imaging acquisition device 4, and the region of interest of the sample is determined.
Preferably, the movement of the sample may be dependent on the first imaging information acquired by the first imaging acquisition device 4. The operator determines the region of interest of the specimen by adjusting the movement of the specimen relative to the objective lens 1 at least in accordance with the first imaging information acquired by the first imaging acquisition device 4. That is, it is determined whether the position of the specimen with respect to the objective lens 1 is the region of interest of the specimen by the first imaging information acquired by the first imaging acquisition apparatus 4; if the position of the sample relative to the objective lens 1 is determined to be the region of interest of the sample, the first imaging information acquired by the first imaging acquisition device 4 can be processed by the processing means to form first image information, and the second imaging information acquired by the second imaging acquisition device 7 at the moment can be processed by the processing means to form second image information; if the position of the sample relative to the objective lens 1 is determined not to be the region of interest of the sample, the movement of the sample relative to the objective lens 1 needs to be adjusted until the position of the sample relative to the objective lens 1 is determined to be the region of interest of the sample through the first imaging information acquired by the first imaging acquisition device 4 and then is stopped, in this state, the first imaging information acquired by the first imaging acquisition device 4 is processed by the processing device to form first image information, and the second imaging information acquired by the second imaging acquisition device 7 at this time is processed by the processing device to form second image information.
In the present embodiment, the basis for adjusting the movement of the sample relative to the objective lens 1 is the first imaging information acquired by the first imaging acquisition device 4.
Of course, the movement may also be performed in accordance with the second imaging information pair acquired by the second imaging acquisition apparatus 7. However, since the second imaging information acquired by the second imaging acquisition device 7 should fall into the region of interest of the sample, and since the multiple of the second imaging information is higher than the multiple of the first imaging information, the difficulty of determining the region of interest of the sample by the second imaging information acquired by the second imaging acquisition device 7 is greater than the difficulty of determining the region of interest of the sample by the first imaging information acquired by the first imaging acquisition device 4.
The region of interest of the sample is a suitable region for the sample in the operation process of the sample reading device, and the suitable region of the sample is determined according to the type of the sample and the requirement of the sample reading operation, which are not described in detail one by one and are all within the protection range.
Further, after the region of interest of the sample is determined, second imaging information acquired by the second imaging acquisition device 7 captures the target tissue, and the second imaging acquisition device 7 forms second image information; when the region of interest of the sample is determined, the imaging information acquired by the second imaging acquisition device 7 does not capture the target tissue, and the sample is adjusted to move relative to the objective lens 1 according to the second imaging information acquired by the second imaging acquisition device 7 until the second imaging information acquired by the second imaging acquisition device 7 captures the target tissue, and the second imaging acquisition device 7 forms second image information. By the above adjustment operation, formation of the second image information is further facilitated.
Further, the first image acquisition device 4 forms the first image information when the region of interest of the sample is determined. That is, in this process, the first image acquisition device 4 can be caused to form the first image information regardless of whether the imaging information acquired by the second image acquisition device 7 captures the target tissue. The target tissue may be cells, cell numbers, or the like in a region of interest of the sample.
It is also possible to let the first image information be formed by the first image capturing device 4 after the second image information acquired by the second image capturing device 7 is captured to the target tissue. At this time, the second image information is formed after the second imaging information is acquired by the second imaging acquisition apparatus 7, and at the same time, the first image information is formed after the first imaging information is acquired by the first imaging acquisition apparatus 4.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (14)
1. A specimen slide reading device, comprising:
the multi-time imaging device at least comprises a low-time light path and a high-time light path, and comprises an objective lens (1), a light splitting device (2), a first ocular (3), a second ocular (6), a first imaging acquisition device (4) and a second imaging acquisition device (7); the imaging information of the sample passes through the objective lens (1) and then generates the same initial imaging information in the first direction and the second direction respectively under the action of the light splitting device (2); the object lens (1) and the light splitting device (2) form an objective lens group; the low power optical path is composed of the objective lens group, the first ocular lens (3) and the first imaging acquisition equipment (4), and the first imaging acquisition equipment (4) acquires first imaging information of initial imaging information in the first direction after the initial imaging information passes through the first ocular lens (3); the high power light path is composed of the objective lens group, the second ocular lens (6) and the second imaging acquisition equipment (7), and the second imaging acquisition equipment (7) acquires second imaging information of the initial imaging information in the second direction after passing through the second ocular lens (6); a multiple of the second imaging information is higher than a multiple of the first imaging information;
sample movement control means for regulating movement of the sample relative to the objective lens (1);
and the processing device is used for processing first image information formed after the first imaging acquisition equipment (4) acquires the first imaging information and second image information formed after the second imaging acquisition equipment (7) acquires the second imaging information.
2. The specimen slide reading device according to claim 1, wherein the objective lens group in the low power optical path is a low power objective lens group, and the objective lens group in the high power optical path is a high power objective lens group;
the high-power objective lens group further comprises a magnifying lens group (5), and initial imaging information generated in the second direction under the action of the light splitting device (2) passes through the magnifying lens group (5) and then passes through the second eyepiece (6) to form second imaging information.
3. The specimen slide device according to claim 1, wherein the magnification of the first eyepiece (3) is lower than the magnification of the second eyepiece (6).
4. The specimen slide reading device according to claim 1, wherein at least one of the first eyepiece (3) and the second eyepiece (6) is a replaceable eyepiece.
5. The specimen slide reading apparatus according to claim 1, wherein said first imaging acquisition device (4) and said second imaging acquisition device (7) are cameras;
the first image information and the second image information are pictures formed after the camera shoots.
6. The specimen slide reading device according to claim 1, wherein the spectroscopic device (2) is a spectroscope.
7. The specimen interpreting device as claimed in claim 6, wherein said beam splitter is an achromatic beam splitter.
8. The specimen interpreting device as claimed in claim 1, characterized in that the object lens (1) is an object lens with magnification.
9. The specimen slide reading device of claim 1,
the first ocular (3) and the first imaging acquisition equipment (4) are positioned on an imaging path of the first direction;
the second ocular (6) and the second imaging acquisition device (7) are located on an imaging path in the second direction.
10. The sample streaking device as in one of claims 1 to 9 wherein the sample is a biological sample smear.
11. A sample reading method is characterized by comprising the following steps:
the imaging information of the sample passes through the objective lens (1) and then generates the same initial imaging information in the first direction and the second direction respectively; acquiring first imaging information of the initial imaging information in the first direction after passing through the first ocular (3), and acquiring second imaging information of the initial imaging information in the second direction after passing through the second ocular (6); a multiple of the second imaging information is higher than a multiple of the first imaging information;
-adjusting the sample to move relative to the objective lens (1);
and processing first image information formed after the first imaging information is obtained and second image information formed after the second imaging information is obtained.
12. The specimen slide interpreting method as claimed in claim 11, characterized in that the specimen is adjusted to move relative to the objective lens (1) at least according to the first imaging information acquired by the first imaging acquiring device (4), and the region of interest of the specimen is determined.
13. The sample scoring method as recited in claim 12, wherein, after determining the region of interest of the sample, second imaging information acquired by the second imaging acquisition device (7) captures a target tissue, the second imaging acquisition device (7) forming the second image information;
when the region of interest of the sample is determined, the imaging information acquired by the second imaging acquisition device (7) does not capture the target tissue, the sample is adjusted to move relative to the objective lens (1) according to the second imaging information acquired by the second imaging acquisition device (7) until the second imaging information acquired by the second imaging acquisition device (7) captures the target tissue, and the second imaging acquisition device (7) forms the second image information.
14. The specimen interpretation method according to claim 12 or 13, characterized in that said first imaging acquisition device (4) forms said first image information when determining the region of interest of said specimen;
or, the first imaging acquisition device (4) forms the first image information after the second imaging information acquired by the second imaging acquisition device (7) captures the target tissue.
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