CN115918643B - Tissue embryo low-temperature fresh-keeping storage device - Google Patents
Tissue embryo low-temperature fresh-keeping storage device Download PDFInfo
- Publication number
- CN115918643B CN115918643B CN202211593551.4A CN202211593551A CN115918643B CN 115918643 B CN115918643 B CN 115918643B CN 202211593551 A CN202211593551 A CN 202211593551A CN 115918643 B CN115918643 B CN 115918643B
- Authority
- CN
- China
- Prior art keywords
- temperature
- inner cavity
- box body
- main box
- culture dish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 20
- 230000007246 mechanism Effects 0.000 claims abstract description 41
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 21
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 11
- 230000001960 triggered effect Effects 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 230000009471 action Effects 0.000 description 11
- 230000007774 longterm Effects 0.000 description 6
- 239000003570 air Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a low-temperature fresh-keeping storage device for tissue embryos, and belongs to the technical field of tissue embryo storage. The low-temperature fresh-keeping storage device for the tissue embryo comprises a main box body, a transmission shaft and a rotary table, and further comprises: a plurality of clamping grooves; the storing and taking mechanism comprises a rotating shaft, a bracket, a bearing table and a sliding rod bearing table, wherein the bearing table is used for bearing the culture dish, a sliding groove is formed in the bearing table, a hydraulic cylinder is arranged on the bearing table, the output end of the hydraulic cylinder is connected with the sliding rod, the sliding rod is in sliding connection with the sliding groove, a stop block is arranged at one end, close to the rotating shaft, of the sliding rod, and an electric lifting mechanism is arranged at one end, far away from the rotating shaft, of the sliding rod; the pre-storing box is provided with a through groove; and the two output ends of the refrigerating device are respectively communicated with the inner cavity of the main box body and the inner cavity of the pre-storing box. The low-temperature fresh-keeping storage device for tissue embryos can prevent the temperature fluctuation of the inner cavity of the main box body caused by the pre-stored culture dishes with higher temperature, thereby preventing the temperature fluctuation from influencing the tissue embryos in the stored culture dishes.
Description
Technical Field
The invention relates to the technical field of tissue embryo storage, in particular to a tissue embryo low-temperature fresh-keeping storage device.
Background
Histology is the science of the study of the microstructure of the body and its related functions at the tissue, cellular, subcellular and molecular levels, developing from macroscopic to microscopic on an anatomical basis. Embryology is a biological branch discipline that studies morphological changes during the development of animals in individuals, and describes how an embryo develops from a fertilized egg, thereby understanding the characteristics and regularity of various animal development. Histoembryology is a study of tissue cells or embryos that requires the use of large numbers of tissue cells or embryos for the study of tissue embryos. While tissue cells and embryos require cryogenic storage for long term storage, thereby preserving the viability of the tissue cells or embryos. Therefore, long-term safe storage of tissue cells or embryos by means of a low temperature fresh-keeping storage device is required.
The constant low-temperature environment plays an important role in preserving tissue cells or embryos, and the constant-temperature preservation can stably inhibit the activities of the cells and enzymes, so that the cells are in a dormant state for a long time, and the preservation time of the tissue cells or embryos can be greatly prolonged.
The existing tissue embryo low-temperature fresh-keeping storage device generally comprises a storage box body and a refrigerating device, wherein the refrigerating device is communicated with the inner cavity of the box body so as to refrigerate the inner cavity of the main box body. When new culture dishes are stored, the door of the storage box body is required to be opened, and then the culture dishes at normal temperature are placed in the storage box body to finish the storage of the culture dishes. In the process, after the normal-temperature pre-stored culture dish is placed in the box body, a temperature difference exists between the culture dish and the inner cavity of the box body, so that heat is radiated to the inner cavity of the box body, and the temperature of the inner cavity of the box body is affected. Moreover, when the box door of the box body is opened, ambient air enters the inner cavity of the box body, the temperature in the box body can be changed, and the temperature fluctuation of the inner cavity of the box body can be caused by the combined influence of the ambient air and the temperature fluctuation, so that a constant low-temperature environment cannot be created for tissue embryos, the activities of cells and enzymes are influenced, and the long-term preservation of the tissue embryos is not facilitated.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a tissue embryo low-temperature fresh-keeping storage device which can pre-cool a pre-stored culture dish and prevent the temperature fluctuation of the inner cavity of a main box body caused by the pre-stored culture dish in a normal temperature state. In addition, the device stores the pre-culture dish in the main box body from the pre-storage box, and after the pre-storage box pre-cools the pre-stored culture dish, the temperature in the pre-storage box is consistent with the temperature in the main box body, so that the problem that normal-temperature air enters the main box body in the process of storing the culture dish is avoided, and the fluctuation of the temperature in the inner cavity of the main box body is avoided. In conclusion, the device can still ensure that the temperature of the inner cavity of the main box body is relatively constant when the culture dish is stored, and a constant low-temperature environment is created for tissue embryos, so that the long-term storage of the tissue embryos is facilitated.
The invention provides a tissue embryo low-temperature fresh-keeping storage device, which comprises a main box body, a rotary table and a refrigerating device, and further comprises:
the clamping grooves are arranged on the rotary table and are used for clamping the culture dishes;
The storage mechanism is arranged in the inner cavity of the main box body and comprises a rotating shaft, a support, a bearing table and a sliding rod, wherein the rotating shaft is connected with a main box body bearing, the support is connected with the rotating shaft, the bearing table is in sliding connection with the support, the bearing table is used for bearing a culture dish, a sliding groove is arranged on the bearing table, the sliding rod is in sliding connection with the sliding groove, a hydraulic cylinder is arranged on the bearing table, the hydraulic cylinder is communicated with a hydraulic control loop, the output end of the hydraulic cylinder is connected with the sliding rod, a stop block is arranged at one end of the sliding rod close to the rotating shaft, and an electric lifting mechanism is arranged at one end of the sliding rod far away from the rotating shaft;
The pre-storing box is arranged on one side of the main box body, a through groove is formed in the main box body, an inner cavity of the pre-storing box is communicated with the inner cavity of the main box body through the through groove, the bearing table can penetrate through the through groove, and the inner cavity of the pre-storing box is communicated with the refrigerating device;
Placing the prestored culture dish on the slide bar of the access mechanism, closing the door of the prestored box, controlling the refrigerating device to refrigerate into the prestored box until the temperatures of the prestored box, the culture dish and the main box are the same, and controlling the electric lifting mechanism, the rotating shaft, the hydraulic cylinder and the bearing table of the access mechanism to act, thereby utilizing the access mechanism to store the prestored culture dish in the prestored box in the clamping groove on the turntable.
Preferably, the main tank body cavity is provided with a first temperature sensor, the first temperature sensor is electrically connected with a controller, the pre-storing tank cavity is provided with a second temperature sensor, the second temperature sensor is electrically connected with the controller, the controller is electrically connected with the refrigerating device, the first temperature sensor is used for detecting the real-time temperature of the main tank cavity, the second temperature sensor is used for detecting the real-time temperature of the pre-storing tank cavity, a preset temperature threshold value is preset in the controller, when the real-time temperature of the main tank cavity is lower than the preset temperature threshold value, the controller controls the refrigerating device to stop refrigerating for the main tank cavity, and when the real-time temperature of the pre-storing tank cavity is lower than the preset temperature threshold value, the controller controls the refrigerating device to stop refrigerating for the pre-storing tank cavity.
Preferably, the through groove is provided with an electric door, the electric door is electrically connected with the controller, and the controller controls the electric door to be opened when the real-time temperature of the inner cavity of the pre-storage box is the same as the real-time temperature of the inner cavity of the main box in the process of storing the culture dish.
Preferably, one end, far away from the rotating shaft, of the sliding rod is provided with a travel switch, the travel switch is electrically connected with the electric door and used for controlling the electric door to be closed, the bottom end of the rotary table is provided with a contact block, and when the travel switch is triggered by the contact block, the electric door is closed.
Preferably, the travel switch is electrically connected with the electric lifting mechanism, and the electric lifting mechanism descends when the travel switch is triggered by the touch block.
Preferably, the inner wall of the main box body and the inner wall of the pre-storing box are provided with heat insulation layers
Preferably, the transmission shaft is vertically arranged, a plurality of rotary tables are arranged on the transmission shaft, the support is connected with the rotating shaft in a sliding mode, the support is provided with a threaded hole, a connecting piece is arranged on the rotating shaft, a screw rod is connected to the connecting piece in a bearing mode, the screw rod is connected with a first power device, and the threaded hole is in threaded connection with the screw rod.
Preferably, a screw rod is arranged on the support, a nut sleeve is arranged at the bottom end of the bearing table, the screw rod is in threaded connection with the nut sleeve, and a second power device is connected to the screw rod.
Preferably, the side walls of the clamping grooves are respectively provided with a spring thimble, the needle heads of the spring thimbles are abutted with the outer wall of the culture dish, and the needle heads of the spring thimbles are used for limiting the radial movement of the culture dish along the turntable.
Compared with the prior art, the invention has the beneficial effects that: the low-temperature fresh-keeping storage device for tissue embryos can pre-cool the pre-stored culture dishes and prevent the pre-stored culture dishes in a normal temperature state from causing temperature fluctuation of the inner cavity of the main box body. In addition, the device stores the pre-culture dish in the main box body from the pre-storage box, and after the pre-storage box pre-cools the pre-stored culture dish, the temperature in the pre-storage box is consistent with the temperature in the main box body, so that the problem that normal-temperature air enters the main box body in the process of storing the culture dish is avoided, and the fluctuation of the temperature in the inner cavity of the main box body is avoided. In conclusion, the device can still ensure that the temperature of the inner cavity of the main box body is relatively constant when the culture dish is stored, and a constant low-temperature environment is created for tissue embryos, so that the long-term storage of the tissue embryos is facilitated.
By setting the first temperature sensor and the second temperature sensor, the real-time temperature of the main body cavity and the pre-storage cavity is controlled, so that the real-time temperature of the main body cavity and the pre-storage cavity is always maintained at a proper preset temperature threshold value, and the tissue embryo is always in a proper low-temperature fresh-keeping environment. Through setting up the electrically operated gate, when the real-time temperature of pre-storing case inner chamber is the same with the real-time temperature of main tank body inner chamber, the controller control electrically operated gate opens, can guarantee to put into the in-process in the main tank with the pre-culture dish of pre-storing the incasement, and the temperature in the main tank body is unanimous with the temperature in the pre-storing case to further prevent the temperature fluctuation in the main tank body, guarantee that the temperature is invariable in the main tank body. Through setting up travel switch, when the culture dish is deposited to the draw-in groove in, travel switch is triggered for the electrically operated gate is in time closed, thereby makes pre-storing case inner chamber and main tank inner chamber separation, prevents that the temperature of pre-storing case inner chamber from causing the influence to the temperature of main tank inner chamber, further guarantees that main tank inner chamber temperature is invariable. The travel switch is utilized to control the action of the electric lifting mechanism simultaneously, when the culture dish is extracted from the pre-storing box, the output end of the electric lifting mechanism is controlled to rise, the culture dish is taken out, and under the action of the output end of the electric lifting mechanism, the sliding rod and the culture dish can be prevented from sliding relatively, so that the normal operation of the device is ensured. Through setting up the heat preservation, utilize the heat preservation to hinder the heat to main tank body, can guarantee that main tank body keeps lower temperature. Through setting up a plurality of revolving stages, lead screw and first power device, can promote the ability of depositing of this device to the culture dish to promote the storage performance of whole device. Through setting up the lead screw, utilize second power device drive lead screw action to drive screw cover action utilizes screw cover drive plummer action, can promote the degree of automation of whole device, thereby promotes the use convenience of this device. Through setting up the spring thimble, after the culture dish is advanced the draw-in groove, the spring thimble pops out to with the firm joint of culture dish in the draw-in groove, thereby can prevent that the transmission shaft from throwing away the draw-in groove with the culture dish when rotating.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic view of the structure of the A-A surface of the present invention;
FIG. 3 is a schematic view of the structure of the B-B surface of the present invention;
FIG. 4 is a schematic view of the structure of the present invention when a culture dish is present;
Fig. 5 is a schematic view of the structure of the travel switch of the present invention.
Reference numerals illustrate:
101. Main box 102, drive shaft 103, turntable 104, clamping groove 105, culture dish 106, rotary shaft 107, bracket 108, bearing platform 109, slide bar 110, slide groove 111, hydraulic cylinder 112, pre-storage box 113, through groove 114, electric door 115, stop 116, electric door 201, connecting piece 202, screw rod 203, first power device 301, first temperature sensor 302, second temperature sensor 401, travel switch 402, contact block 5, screw rod 6, spring thimble 7 and heat preservation.
Detailed Description
Specific embodiments of the present invention will be described in detail below with reference to fig. 1-5, but it should be understood that the scope of the present invention is not limited by the specific embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1:
As shown in fig. 1-4, the low-temperature fresh-keeping storage device for tissue embryos provided by the invention comprises a main box body 101, a transmission shaft 102, a turntable 103 and a refrigerating device, and further comprises: the plurality of clamping grooves 104, the access mechanism and the pre-storage box 112, wherein the plurality of clamping grooves 104 are arranged on the turntable 103, and the plurality of clamping grooves 104 are used for clamping the culture dish 105; the access mechanism is arranged in the inner cavity of the main box body 101 and comprises a rotating shaft 106, a support 107, a bearing table 108 and a sliding rod 109, wherein the rotating shaft 106 is connected with the main box body 101 through a bearing, the support 107 is connected with the rotating shaft 106, the bearing table 108 is slidably connected with the support 107, the bearing table 108 is used for receiving the culture dish 105, a chute 110 is arranged on the bearing table 108, the sliding rod 109 is slidably connected with the chute 110, a hydraulic cylinder 111 is arranged on the bearing table 108, the hydraulic cylinder 111 is communicated with a hydraulic control loop, the output end of the hydraulic cylinder 111 is connected with the sliding rod 109, a stop block 115 is arranged at one end of the sliding rod 109 close to the rotating shaft 106, and an electric lifting mechanism 114 is arranged at one end of the sliding rod 109 far away from the rotating shaft 106; the pre-storing box 112 is arranged on one side of the main box body 101, a through groove 113 is formed in the main box body 101, an inner cavity of the pre-storing box 112 is communicated with the inner cavity of the main box body 101 through the through groove 113, the bearing table 108 can penetrate through the through groove 113, the inner cavity of the pre-storing box 112 is communicated with the refrigerating device, the pre-stored culture dish 105 is placed on the sliding rod 109 of the access mechanism, a box door of the pre-storing box 112 is closed, the refrigerating device is controlled to refrigerate into the pre-storing box 112 until the temperatures of the pre-storing box 112, the culture dish 105 and the main box body 101 are the same, and the electric lifting mechanism 114, the rotating shaft 106, the hydraulic cylinder 111 and the bearing table 108 of the access mechanism are controlled to act, so that the pre-stored culture dish 105 in the pre-storing box 112 is stored in the clamping groove 104 on the rotary table 103 by the access mechanism.
The working principle of example 1 will now be briefly described:
The transmission shaft 102 of this device is located in the main tank 101, and the revolving stage 103 is located on the transmission shaft 102, and refrigerating plant has two output, refrigerating plant's two output respectively with main tank 101 inner chamber and prestoring case 112 inner chamber intercommunication, this device is when using, rotates pivot 106 to drive plummer 108 rotation, until plummer 108 rotates to one side towards logical groove 113, slides plummer 108, until plummer 108 passes logical groove 113. The output end of the control hydraulic cylinder 111 stretches out to drive the sliding rod 109 to slide out of the bearing table 108 until an electric lifting mechanism 114 on the sliding rod 109 is abutted with the inner side wall of the pre-storing box 112. At this time, the door of the pre-tank 112 is opened, the inner cavity of the pre-tank 112 is communicated with the atmosphere, and the air temperature in the pre-tank 112 is raised. Placing the pre-stored culture dish 105 with the tissue embryo on the slide bar 109, closing the door of the pre-stored box 112, controlling the refrigerating device to refrigerate into the pre-stored box 112 until the temperature in the pre-stored box 112 is the same as the temperature in the main box 101, and at the moment, the temperature of the pre-stored culture dish 105 is the same as the temperature in the main box 101. At this time, the output end of the electric lifting mechanism 114 is controlled to rise, and the output end of the hydraulic cylinder 111 is controlled to shrink, so that the slide rod 109 is driven to shrink onto the bearing table 108, the prestored culture dish 105 is driven to move onto the bearing table 108 under the action of the output end of the electric lifting mechanism 114, and the bearing table 108 is slid, so that the bearing table 108 is drawn back from the through groove 113, and the bearing table 108 approaches the rotating shaft 106. The rotating shaft 106 is rotated to drive the bearing table 108 to rotate until the bearing table 108 rotates to the side facing the clamping groove 104 of the turntable 103. And then sliding the bearing table 108 until the bearing table 108 is close to the turntable 103, controlling the output end of the hydraulic cylinder 111 to extend, and driving the culture dish 105 on the slide rod 109 to slide into the clamping groove 104 in the same way, so as to finish the storage of the culture dish 105. When the stored culture dish 105 is used, the reverse operation is performed.
The low-temperature fresh-keeping storage device for tissue embryos can pre-cool the pre-stored culture dishes and prevent the pre-stored culture dishes in a normal temperature state from causing temperature fluctuation of the inner cavity of the main box body. In addition, the device stores the pre-culture dish 105 into the main box body 101 from the pre-storage box 112, and after pre-cooling the pre-stored culture dish 105, the temperature in the pre-storage box 112 is consistent with the temperature in the main box body 101, so that the problem that normal-temperature air enters the main box body 101 in the process of storing the culture dish 105 is avoided, and the fluctuation of the temperature in the inner cavity of the main box body 101 is avoided. In summary, the device can still ensure that the temperature in the inner cavity of the main box 101 is relatively constant when the culture dish 105 is stored, and a constant low-temperature environment is created for the tissue embryo, thereby being beneficial to long-term storage of the tissue embryo.
Example 2:
On the basis of example 1, in order to maintain the real-time temperature of the inner cavity of the main casing 101 and the inner cavity of the pre-storing casing 112 at the appropriate predetermined temperature threshold value all the time, the activity of the tissue embryo in the stored culture dish 105 is ensured.
As shown in fig. 2 to 4, the inner cavity of the main box 101 is provided with a first temperature sensor 301, the first temperature sensor 301 is electrically connected with a controller, the inner cavity of the pre-storage box 112 is provided with a second temperature sensor 302, the second temperature sensor 302 is electrically connected with the controller, the controller is electrically connected with the refrigerating device, the first temperature sensor 301 is used for detecting the real-time temperature of the inner cavity of the main box 101, the second temperature sensor 302 is used for detecting the real-time temperature of the inner cavity of the pre-storage box 112, a preset temperature threshold value is preset in the controller, when the real-time temperature of the inner cavity of the main box 101 is lower than the preset temperature threshold value, the controller controls the refrigerating device to stop refrigerating to the inner cavity of the main box 101, and when the real-time temperature of the inner cavity of the pre-storage box 112 is lower than the preset temperature threshold value, the controller controls the refrigerating device to stop refrigerating to the inner cavity of the storage box.
As the refrigerating device works, the real-time temperature of the inner cavity of the main box body 101 and the inner cavity of the pre-storing box 112 is reduced, the first temperature sensor 301 detects the real-time temperature of the inner cavity of the main box body 101, and when the real-time temperature of the inner cavity of the main box body 101 is lower than a preset temperature threshold value, the controller controls the refrigerating device to stop refrigerating to the inner cavity of the main box body 101; the second temperature sensor 302 detects the real-time temperature of the inner cavity of the pre-storage box 112, and when the real-time temperature of the inner cavity of the pre-storage box 112 is lower than the preset temperature threshold, the controller controls the refrigerating device to stop refrigerating the inner cavity of the pre-storage box 112, so that the real-time temperature of the inner cavity of the main box 101 and the inner cavity of the pre-storage box 112 is always maintained at the proper preset temperature threshold, and the activity of the tissue embryo in the stored culture dish 105 is further ensured.
Example 3:
On the basis of embodiment 2, in order to prevent the real-time temperature of the inner cavity of the pre-storage tank 112 from affecting the temperature in the main tank 101, the temperature in the main tank 101 is further ensured to be constant.
As a preferred solution, as shown in fig. 1, the through slot 113 is provided with an electric door 116, the electric door 116 is electrically connected with the controller, and the controller controls the electric door 116 to open when the real-time temperature of the cavity of the pre-storage box 112 is the same as the real-time temperature of the cavity of the main box 101 during the process of storing the culture dish 105.
Through setting up electrically operated gate 116, when the real-time temperature of pre-storing case 112 inner chamber is the same with the real-time temperature of main tank body 101 inner chamber, controller control electrically operated gate 116 opens, can guarantee to put the in-process of main tank body 101 into the pre-culture dish of pre-storing case 112 in, prevents that the real-time temperature of pre-storing case 112 inner chamber from causing the influence to the temperature in the main tank body 101 to further guarantee that the temperature is invariable in the main tank body 101.
As a preferred solution, as shown in fig. 1 to 5, one end of the sliding rod 109 far away from the rotating shaft is provided with a travel switch 401, the travel switch 401 is electrically connected with the electric door 116, the travel switch 401 is used for controlling the electric door 116 to be closed, the bottom end of the turntable 103 is provided with a contact block 402, and when the travel switch 401 is triggered by the contact block 402, the electric door 116 is closed. When the culture dish 105 is stored, the electric door 116 is controlled to be opened, so that the carrying table 108 can pass through the through groove 113. When the bearing table 108 takes the culture dish 105 out of the pre-storing box 112 and stores the culture dish 105 on the rotary table 103, the slide bar 109 pushes the culture dish 105 to move towards the clamping groove 104, when the culture dish 105 is stored in the clamping groove 104, the travel switch 401 on the slide bar 109 is abutted with the contact block 402 at the bottom end of the rotary table 103, so that the travel switch 401 is triggered, the electric door 116 is closed in time, the inner cavity of the pre-storing box 112 is blocked from the inner cavity of the main box 101, the influence of the temperature of the inner cavity of the pre-storing box 112 on the temperature of the inner cavity of the main box 101 is further prevented, and the constant temperature of the inner cavity of the main box 101 is further ensured.
As a preferred embodiment, as shown in fig. 1-5, the travel switch 401 is electrically connected to the electric lifting mechanism 114, and the electric lifting mechanism 114 descends when the travel switch 401 is triggered by the contact block 402. In the process of storing the culture dish 105, when the slide bar 109 is used for pushing the culture dish 105 to be stored in the clamping groove 104, when the slide bar 109 stretches to the point that a travel switch 401 on the slide bar 109 is contacted with a contact block 402 at the bottom end of the turntable 103, the culture dish 105 is stored in the clamping groove 104, at the moment, the travel switch 401 is triggered, and the travel switch 401 controls the electric lifting mechanism 114 to descend, so that the electric lifting mechanism 114 is prevented from obstructing the reset of the slide bar 109 when the slide bar 109 is reset. In the process of taking the culture dish 105, the hydraulic cylinder 111 is controlled to move until the travel switch 401 on the slide rod 109 is contacted with the contact block 402 at the bottom end of the turntable 103, the travel switch 401 is triggered, and the travel switch 401 controls the electric lifting mechanism 114 to ascend, so that the culture dish 105 in the clamping groove 104 is taken out by the output end of the electric lifting mechanism 114. The travel switch 401 is utilized to control the electric lifting mechanism 114 to act simultaneously, when the culture dish 105 is extracted from the pre-storing box 112, the output end of the electric lifting mechanism 114 is controlled to rise, the culture dish 105 is taken out, and under the action of the output end of the electric lifting mechanism 114, the slide rod 109 and the culture dish 105 can be prevented from sliding relatively, so that the normal operation of the device is ensured.
As a preferred solution, as shown in fig. 1-4, the inner wall of the main box 101 and the inner wall of the pre-storage box 112 are provided with heat insulation layers 7. By arranging the heat preservation layer 7, the heat preservation layer 7 is utilized to block heat of the main box body 101, and the main box body 101 can be ensured to maintain a lower temperature.
As a preferable scheme, as shown in fig. 1 and 3, the transmission shaft 102 is vertically arranged, a plurality of turntables 103 are arranged on the transmission shaft 102, the rotating shaft 106 is slidably connected with the bearing table 108, the bearing table 108 is provided with a threaded hole, a connecting piece 201 is arranged on the rotating shaft 106, a vertically arranged screw rod 202 is connected to the connecting piece 201 through a bearing, a first power device 203 is connected to the screw rod 202, and the threaded hole is in threaded connection with the screw rod 202. By providing a plurality of turntables 103, a plurality of clamping grooves 104 are formed in each turntable 103, and culture dishes 105 can be stored in each clamping groove 104. By arranging the screw rod 202 and the first power device 203, the screw rod 202 is controlled to rotate by controlling the action of the first power device 203, so that the bracket 107 and the bearing table 108 are driven to lift, and the culture dishes 105 on different turntables 103 on the transmission shaft 102 are accessed. Therefore, by providing the plurality of turntables 103, the screw 202 and the first power device 203, the storage amount of the whole device to the culture dish 105 can be greatly improved, thereby improving the storage performance of the whole device.
As a preferable solution, as shown in fig. 1 and 3, a screw rod 5 is provided on the support 107, a nut sleeve is provided at the bottom end of the bearing table 108, the screw rod 5 is in threaded connection with the nut sleeve, and a second power device is connected to the screw rod 5. Through setting up lead screw 5, utilize second power device drive lead screw 5 action to drive screw cover action, utilize screw cover drive plummer 108 action, can promote the degree of automation of whole device, thereby promote the use convenience of this device.
As a preferred solution, as shown in fig. 2 and 4, the side walls of the clamping grooves 104 are provided with spring pins 6, the needles of the spring pins 6 are abutted against the outer wall of the culture dish 105, and the needles of the spring pins 6 are used for limiting the radial movement of the culture dish 105 along the turntable 103. Through setting up spring thimble 6, after culture dish 105 is advanced draw-in groove 104, spring thimble 6 pops out to with the firm joint of culture dish 105 in draw-in groove 104, thereby can prevent that transmission shaft 102 from throwing away culture dish 105 from draw-in groove 104 when rotating.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The utility model provides a tissue embryo low temperature strorage device, includes main box (101), revolving stage (103) and refrigerating plant, its characterized in that still includes:
a plurality of clamping grooves (104) arranged on the turntable (103), wherein the clamping grooves (104) are used for clamping the culture dish (105);
The access mechanism is arranged in the inner cavity of the main box body (101), the access mechanism comprises a rotating shaft (106), a support (107), a bearing table (108) and a sliding rod (109), the rotating shaft (106) is connected with the main box body (101) through a bearing, the support (107) is connected with the rotating shaft (106), the bearing table (108) is slidably connected with the support (107), the bearing table (108) is used for bearing a culture dish (105), a sliding groove (110) is formed in the bearing table (108), the sliding rod (109) is slidably connected with the sliding groove (110), a hydraulic cylinder (111) is arranged on the bearing table (108), the hydraulic cylinder (111) is communicated with a hydraulic control loop, the output end of the hydraulic cylinder (111) is connected with the sliding rod (109), one end, close to the rotating shaft (106), of the sliding rod (109) is provided with a stop block (115), and one end, far away from the rotating shaft (106), of the sliding rod (109) is provided with an electric lifting mechanism (114).
The pre-storing box (112) is arranged on one side of the main box body (101), a through groove (113) is formed in the main box body (101), the inner cavity of the pre-storing box (112) is communicated with the inner cavity of the main box body (101) through the through groove (113), the bearing table (108) can penetrate through the through groove (113), and the inner cavity of the pre-storing box (112) is communicated with the refrigerating device;
Placing the prestored culture dish (105) on a slide bar (109) of the access mechanism, closing a door of a prestored box (112), controlling a refrigerating device to refrigerate into the prestored box (112), and controlling an electric lifting mechanism (114), a rotating shaft (106), a hydraulic cylinder (111) and a bearing table (108) of the access mechanism to act when the temperature of the prestored box (112) and the temperature of the culture dish (105) are the same as the temperature of a main box body (101), so that the prestored culture dish (105) in the prestored box (112) is stored in a clamping groove (104) on a turntable (103) by using the access mechanism;
An electric door (116) is arranged on the through groove (113), the electric door (116) is electrically connected with the controller, and when the real-time temperature of the inner cavity of the pre-storage box (112) is the same as the real-time temperature of the inner cavity of the main box body (101) in the process of storing the culture dish (105), the controller controls the electric door (116) to be opened;
A travel switch (401) is arranged at one end, far away from the rotating shaft, of the sliding rod (109), the travel switch (401) is electrically connected with the electric door (116), the travel switch (401) is used for controlling the electric door (116) to be closed, a contact block (402) is arranged at the bottom end of the rotary table (103), and when the travel switch (401) is triggered by the contact block (402), the electric door (116) is closed;
the travel switch (401) is electrically connected with the electric lifting mechanism (114), and when the travel switch (401) is triggered by the contact block (402), the electric lifting mechanism (114) descends;
the screw rod (5) is arranged on the support (107), a screw sleeve is arranged at the bottom end of the bearing table (108), the screw rod (5) is in threaded connection with the screw sleeve, and a second power device is connected to the screw rod (5).
2. The tissue embryo low-temperature fresh-keeping storage device according to claim 1, wherein a first temperature sensor (301) is arranged in the inner cavity of the main box body (101), the first temperature sensor (301) is electrically connected with a controller, a second temperature sensor (302) is arranged in the inner cavity of the pre-storage box (112), the second temperature sensor (302) is electrically connected with the controller, the controller is electrically connected with the refrigerating device, the first temperature sensor (301) is used for detecting the real-time temperature of the inner cavity of the main box body (101), the second temperature sensor (302) is used for detecting the real-time temperature of the inner cavity of the pre-storage box (112), a preset temperature threshold value is preset in the controller, the controller controls the refrigerating device to stop refrigerating to the inner cavity of the main box body (101) when the real-time temperature of the inner cavity of the main box body (101) is lower than the preset temperature threshold value, and controls the refrigerating device to stop refrigerating to the inner cavity of the pre-storage box (112) when the real-time temperature of the inner cavity of the pre-storage box (112) is lower than the preset temperature threshold value.
3. The low-temperature fresh-keeping storage device for tissue embryos according to claim 1, wherein the inner wall of the main box body (101) and the inner wall of the pre-storage box (112) are provided with heat preservation layers (7).
4. The tissue embryo low-temperature fresh-keeping storage device according to claim 1, wherein a transmission shaft (102) is vertically arranged, a plurality of rotary tables (103) are arranged on the transmission shaft (102), a support (107) is connected with a rotating shaft (106) in a sliding mode, the support (107) is provided with a threaded hole, a connecting piece (201) is arranged on the rotating shaft (106), a screw rod (202) which is vertically arranged is connected to an upper bearing of the connecting piece (201), a first power device (203) is connected to the screw rod (202), and the threaded hole is in threaded connection with the screw rod (202).
5. The tissue embryo low-temperature fresh-keeping storage device according to claim 1, wherein the side walls of the clamping grooves (104) are respectively provided with a spring thimble (6), the needle heads of the spring thimbles (6) are abutted with the outer wall of the culture dish (105), and the needle heads of the spring thimbles (6) are used for limiting the radial movement of the culture dish (105) along the turntable (103).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211593551.4A CN115918643B (en) | 2022-12-13 | 2022-12-13 | Tissue embryo low-temperature fresh-keeping storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211593551.4A CN115918643B (en) | 2022-12-13 | 2022-12-13 | Tissue embryo low-temperature fresh-keeping storage device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115918643A CN115918643A (en) | 2023-04-07 |
| CN115918643B true CN115918643B (en) | 2024-04-19 |
Family
ID=86650519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211593551.4A Active CN115918643B (en) | 2022-12-13 | 2022-12-13 | Tissue embryo low-temperature fresh-keeping storage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115918643B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379706A (en) * | 2015-10-22 | 2016-03-09 | 上海原能细胞医学技术有限公司 | Automatic preservation/extraction control device for frozen cells |
| CN209291248U (en) * | 2018-11-27 | 2019-08-23 | 上海原能细胞生物低温设备有限公司 | A kind of biological sample intellectual access system |
| CN111908119A (en) * | 2019-05-08 | 2020-11-10 | 青岛海特生物医疗有限公司 | Low temperature transport method |
| CN212610546U (en) * | 2020-05-27 | 2021-02-26 | 南京铭声通电子科技有限公司 | Storage device of cell culture dish |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1609850A1 (en) * | 2004-06-24 | 2005-12-28 | Biovir v/Jacob Mollenbach | Culture dish for culturing biological cells |
| GB2525884A (en) * | 2014-05-07 | 2015-11-11 | Unisense Fertilitech As | Culture dish |
| US20180112164A1 (en) * | 2016-10-26 | 2018-04-26 | Michael D. Cecchi | Device for Observation, Imaging and Uninterrupted Culturing of Embryos and Cells |
-
2022
- 2022-12-13 CN CN202211593551.4A patent/CN115918643B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379706A (en) * | 2015-10-22 | 2016-03-09 | 上海原能细胞医学技术有限公司 | Automatic preservation/extraction control device for frozen cells |
| CN209291248U (en) * | 2018-11-27 | 2019-08-23 | 上海原能细胞生物低温设备有限公司 | A kind of biological sample intellectual access system |
| CN111908119A (en) * | 2019-05-08 | 2020-11-10 | 青岛海特生物医疗有限公司 | Low temperature transport method |
| CN212610546U (en) * | 2020-05-27 | 2021-02-26 | 南京铭声通电子科技有限公司 | Storage device of cell culture dish |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115918643A (en) | 2023-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8083080B2 (en) | Storage apparatus for storing products | |
| AU660190B2 (en) | Storage apparatus, particularly with automatic insertion and retrieval | |
| CN106976674B (en) | Low-temperature storage transfer device and operation method thereof | |
| US20030037556A1 (en) | Automated storage and retrieval apparatus for freezers and related method thereof | |
| CN110436027B (en) | System for storing biological samples | |
| CN107972999A (en) | A kind of freezing storing box storage tank | |
| CN107975997A (en) | A kind of refrigerator of automatic access food materials | |
| CN115918643B (en) | Tissue embryo low-temperature fresh-keeping storage device | |
| CN205431772U (en) | Automatic storing and taking device for preventing freezing injury of placenta stem cells | |
| CN205124851U (en) | Cell freezing storage device | |
| CN204438667U (en) | The rotatable constant temperature cabinet of a kind of matter storage lattice | |
| CN111217070B (en) | Can automatic access sample's breeding device | |
| CN107972967B (en) | Low-temperature storage tank | |
| CN212100185U (en) | Storage device | |
| CN210569466U (en) | Full-automatic sample storehouse refrigerator of-80 degrees centigrade | |
| CN208987621U (en) | A kind of shaft lift low-temperature storage device | |
| CN208987622U (en) | A kind of spiral lift low-temperature storage device | |
| CN220701935U (en) | Preservation device of freeze-dried powder | |
| CN205386814U (en) | Automatic grabbing device of stem cell cryopreserving box | |
| CN219674536U (en) | Medicine refrigerator | |
| CN220507353U (en) | Material storage device | |
| CN111374121A (en) | Separated liquid nitrogen storage system and control method thereof | |
| KR20190130352A (en) | A Meat Stock Slush Manufacturing Method | |
| CN219146581U (en) | Storage device for stem cell sampling | |
| CN214567076U (en) | Biological sample ware cache storehouse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |