CN115908211A

CN115908211A - Sample reading device and method

Info

Publication number: CN115908211A
Application number: CN202111114746.1A
Authority: CN
Inventors: 姜斌
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2021-09-23
Filing date: 2021-09-23
Publication date: 2023-04-04

Abstract

The embodiment of the invention discloses a sample reading device and a method, wherein the sample reading device comprises: the device comprises a light source component, a sample component, an imaging device and a processor, wherein the light source component generates light of at least four spectral bands, the light of the at least four spectral bands is sequentially irradiated on a sample carried by the sample component, the imaging device acquires imaging information of the sample respectively under the irradiation of the light of the at least four spectral bands to generate a sample image, and the processor fuses the sample images obtained under the action of the at least four spectral bands. Therefore, the colors of the image obtained by the sample interpreting device are obtained under the adjustment of the colors of at least four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced. Moreover, the structure for acquiring the sample image by the sample reading device is simple and easy to realize.

Description

Sample reading device and method

Technical Field

The invention relates to the field of image processing, in particular to a sample reading device and a sample reading method.

Background

The cell morphology analyzer, also called a sheet reader, is an instrument device for analyzing cells on smears of peripheral blood, bone marrow, body fluid and the like, and the sheet reader mainly has the working principle of automatically identifying a single-layer cell area based on a microscopic optical and digital shooting module and applying an intelligent image processing algorithm, searching and shooting blood cells (the blood cells comprise white blood cells, red blood cells, platelets and the like) in the single-layer cell area, and then scratching out a single-cell image. After the single cell image is subjected to necessary image processing, the type and the characteristics of the cell are identified through an intelligent identification algorithm. And classifying the cells according to the characteristics of the cells, and displaying the cell images on a display. The operation user can adjust the instrument classification result according to clinical experience and patient related information and give corresponding clinical conclusion.

When the cell image is shot, the cell needs to be moved to the vicinity of the focusing surface of the objective lens, one or more images are shot, and subsequent image enhancement processing is carried out to finally obtain a clear cell microscopic image. The finally obtained image not only reflects the internal texture details clearly, but also reflects the color characteristics of cells accurately, however, the currently obtained image generally has the problem of color distortion.

Disclosure of Invention

In view of this, the embodiment of the present invention discloses a sample reading device and method, which fuse images obtained by a sample under the irradiation of light of at least four spectral bands, so as to obtain an image with higher color accuracy.

The embodiment of the invention discloses a sample reading device, which comprises:

a light source component for generating at least four bands of light encompassing at least the visible light spectral range;

a sample part for carrying a sample;

an imaging device that sequentially irradiates the sample carried by the sample member with light of at least four spectral bands generated by the light source member, captures imaging information of the sample under the irradiation of the light of each spectral band, and generates an image of the sample based on the imaging information of the sample under the irradiation of the light of each spectral band;

and the processor is used for fusing the sample images obtained under the action of at least four spectral bands.

Optionally, the processor is further configured to:

fusing sample images obtained under the action of the at least four spectral bands based on the weight of each spectral band in the at least four spectral bands;

the weight of each of the at least four spectral bins is determined by:

adjusting weights of at least four spectral bands of a first image of a reference sample such that colors of the first image match colors of a second image of the reference sample, determining a weight for each of the at least four spectral bands; the second image is an image with a color display effect meeting a preset condition, and the first image is an image with a color display effect not meeting the preset condition

Optionally, the light source component includes: a first light source generator and a light source switching part;

the first light source generator is used for generating light in different spectral bands;

the light source switching component is used for controlling the spectrum section of the light generated by the first light source generator so as to switch the light emitted by the first light source generator.

Optionally, the light source component includes: a second light source generator and a filter member;

the second light source generator is used for generating light of a continuous spectrum;

the filtering component is used for filtering the light passing through the filtering component to obtain light with different spectral bands.

Optionally, the filtering component includes: an optical filter and an optical filter switching member;

the optical filter comprises at least four optical filters, and the spectral bands filtered by each optical filter in the at least four optical filters are different;

the optical filter switching component is used for controlling the optical filter in the optical filter to switch so that the switched optical filter is positioned on the light path from the light source component to the sample.

Optionally, the sample part comprises:

a sample movement control module to adjust a position of the sample relative to an imaging device to enable the imaging device to acquire a region of interest of the sample on the sample member.

a light source section for generating light to be irradiated on a sample;

a sample part for carrying a sample;

the first imaging device captures imaging information of a sample on a first optical path to obtain a first image; the imaging information on the first optical path is generated by the sample under irradiation of continuous spectrum light generated by a light source part;

a second imaging device capturing imaging information of the sample on a second optical path, resulting in a second image, the imaging information on the second optical path being generated by the sample under the action of light of a first target spectrum band, the light of the first target spectrum band being light of a specific spectrum band;

and the processor is used for fusing the first image and the second image.

Optionally, the processor is further configured to:

fusing the first image and the second image based on the weight of the first target spectrum section and the weight of the second target spectrum section; the second target spectrum is a plurality of spectrum bands for determining the color of the image shot by the first imaging device, and the first target spectrum is a specific spectrum band;

the method for obtaining the weight of the first target spectrum segment and the weight of the second target spectrum segment comprises the following steps:

adjusting weights of a second target spectrum segment and a first target spectrum segment of a third image of a reference sample taken by the first imaging device so that colors of the third image match colors of a fourth image of the reference sample, and determining the weights of the first target spectrum segment and the second target spectrum segment; the fourth image is an image with a color display effect meeting a preset condition, and the third image is an image with a color display effect not meeting the preset condition.

Optionally, the light source part includes:

a third light source generator for generating light of a continuous spectrum and light of the first target spectral band;

and the light source switching device is used for controlling the spectrum section of the light generated by the third light source generator so as to switch the light emitted by the third light source generator.

Optionally, the method further includes:

a light splitting part and a light filtering part;

the light splitting part is arranged on a third light path and disperses the light with continuous spectrum generated by the light source part to the first light path and the second light path;

the filtering component is arranged on the second light path and is used for filtering the light on the second light path so as to obtain the light with a specific spectrum band under the action of the filtering component.

Optionally, the first imaging device is a color camera, and the second imaging device is a grayscale camera.

The embodiment of the invention discloses a sample reading method, which comprises the following steps:

acquiring imaging information of a sample under the light irradiation of at least four spectral bands in sequence to obtain a plurality of sample images; each sample image is generated based on imaging information of the sample under illumination by light of one spectral band, the light of the at least four spectral bands covering at least a visible light spectral range;

and fusing a plurality of sample images obtained under the action of at least four spectral bands.

Optionally, the method includes:

fusing sample images obtained under the action of at least four spectral bands based on the weight of each spectral band in the four spectral bands;

the weight of each of the at least four spectral bins is determined by:

acquiring imaging information of a biological sample on a first optical path through first imaging equipment to obtain a first image;

acquiring imaging information of the biological sample on a second optical path through second imaging equipment to obtain a second image;

the imaging information on the first optical path is obtained by the sample under the irradiation of continuous spectrum light, the imaging information on the second optical path is obtained by the sample under the action of first target spectrum light, and the first target spectrum light is light in a specific spectrum band;

and fusing the first image and the second image.

Optionally, the method further includes:

fusing the first image and the second image based on the weight of the first target spectrum segment and the weight of the second target spectrum segment; the second target spectrum is a plurality of spectrums which determine the colors of the image shot by the first imaging device, and the first target spectrum is any specific spectrum;

the method for acquiring the weight of the first target spectrum section and the weight of the second target spectrum section comprises the following steps:

adjusting weights of a second target spectrum and a first target spectrum of a third image of a reference sample taken by the first imaging device so that colors of the third image match colors of a fourth image of the reference sample, determining the weights of the first target spectrum and the second target spectrum; the fourth image is an image with a color display effect meeting a preset condition, and the third image is an image with a color display effect not meeting the preset condition.

The embodiment of the invention discloses a sample reading device and a method, wherein the sample reading device comprises: the device comprises a light source component, a sample component, an imaging device and a processor, wherein the light source component generates light of at least four spectral bands, the light of the at least four spectral bands is sequentially irradiated on a sample carried by the sample component, the imaging device acquires imaging information of the sample respectively under the irradiation of the light of the at least four spectral bands to generate a sample image, and the processor fuses the sample images obtained under the action of the at least four spectral bands. Therefore, the colors of the image obtained by the sample reading device are obtained under the adjustment of the colors of at least four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced. Moreover, the structure for acquiring the sample image by the sample reading device is simple and easy to realize.

Furthermore, the sample images obtained under the action of the at least four spectral bands are fused through the weight of each spectral band in the at least four spectral bands, so that the color accuracy of the fused sample images is further improved, the fused sample images are closer to real colors, or the fused sample images meet the requirements of users better.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a specimen reading device provided in embodiment 1 of the present invention;

fig. 2 shows a schematic structural view of the light source section 100;

FIG. 3 is a schematic view showing the structure of an example 1 of a specimen interpreting device;

fig. 4 shows another structural schematic diagram of the light source section 100;

fig. 5 shows a schematic configuration of the filter member 104;

FIG. 6 is a schematic view showing a structure of an example 2 of a specimen interpreting apparatus;

FIG. 7 is a schematic structural diagram showing a specimen interpreting apparatus provided in embodiment 2 of the present invention;

FIG. 8 is a schematic structural view showing example 3 of a specimen interpreting device;

fig. 9 shows a schematic configuration diagram of the light source part 500;

FIG. 10 is a flow chart of a specimen interpretation method provided in embodiment 3 of the present invention;

fig. 11 is a flowchart illustrating a sample reading method provided in embodiment 4 of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1, a schematic structural diagram of a specimen interpreting device provided in embodiment 1 of the present invention is shown, and in this embodiment, the specimen interpreting device includes:

a light source part 100, a sample part 200, an imaging apparatus 300, and a processor 400;

a light source part 100 for generating light of at least four spectral bands;

wherein the light of at least four spectral bands generated by the light source component covers at least the visible light spectral range, but is not limited to only the visible light spectral range.

In the present embodiment, the light of each spectrum band represents narrow spectrum light or wide spectrum light of a different wavelength range. For example, if the light of four spectral bands generated by the light source section is represented as: the spectrum analysis device comprises a first spectrum section, a second spectrum section, a third spectrum section and a fourth spectrum section, wherein the first spectrum section, the second spectrum section, the third spectrum section and the fourth spectrum section are respectively narrow spectrum light or wide spectrum light with different wavelength ranges. The first, second, third and fourth spectral bands may be any four different wavelength ranges, and the different wavelength ranges may have partial spectral bands overlapping or may not overlap at all. However, the light source generated by the light source section is not limited to the light of the above four spectral bands.

For example, the following steps are carried out: the light generated by the light source section includes, for example: red, green-blue, magenta, cyan, yellow 6 bands of light, but is not limited to these 6 bands.

In this embodiment, the light source component generates light of at least four bands in multiple ways, and in different ways, the light source component has different structures, and optionally, this embodiment discloses the following two structures of the light source component:

the first embodiment,

Referring to fig. 2, the light source part 100 includes: a first light source generator 101 and a light source switching section 102;

wherein, the first light source generator 101 is used for generating light of different spectral bands;

the light source switching component 102 is configured to control a spectrum band of light generated by the first light source generator to switch light emitted by the first spectrum generator.

In this embodiment, the first light source generator 101 may be a generator that generates light in a plurality of spectral bands, wherein the generator generates light in at least four spectral bands. Alternatively, the first light source generator 101 may be formed by a plurality of laser generators, each capable of generating light in one spectral band.

Where the first light source generator 101 is a generator capable of generating light of a plurality of spectral bands, the light source switching section 102 may program the first light source generator 101 to generate a spectrum of light. In the case where the first light source generator 101 is configured by a plurality of laser generators, the light source switching part 102 may switch the laser generators generating light sources by a program or by mechanical means.

For example, the following steps are carried out: referring to fig. 3, a schematic structural diagram of the specimen interpreting apparatus based on the first embodiment is shown, wherein the light source unit 100 is capable of generating light of at least four spectral bands, the light generated by the light source unit is irradiated onto the specimen carried by the specimen unit 200, wherein the specimen is arranged on the slide, the imaging device 300 captures imaging information of the specimen under the light irradiation of each spectral band, and generates an image of the specimen based on the imaging information of the specimen under the light irradiation of each spectral band.

The second embodiment:

referring to fig. 4, the light source part 100 includes: a second light source generator 103 and a filter member 104;

the second light source generator 103 is used for generating light with continuous spectrum;

the filtering component 104 is configured to perform filtering processing on the light passing through the filtering component to obtain light in different spectral bands.

In this embodiment, where the continuous spectrum is a complete natural spectrum without the missing visible spectrum, the second light source generator 103 may be a visible light generator capable of generating a complete natural spectrum.

In this embodiment, the filtering component 104 may be any filtering device, for example, a filter, or may be another device capable of filtering a continuous spectrum. The filter member 104 may be disposed above the second light source generator, and light generated from the second light source generator 103 is projected onto the filter member, and light of different spectral bands is obtained through the filter member 104.

In this embodiment, in order to obtain light in at least four spectral bands, after the light generated by the second light source generator is projected onto the filter component, the filter component 104 may include:

a filter 1041 and a filter switching member 1042;

the optical filter 1041 includes at least four optical filters, and a filtered spectrum band of each optical filter in the at least four optical filters is different;

the filter switching component 1042 is configured to control a filter in the filter to switch, so that the switched filter is located on a light path from the light source component to the sample.

In this embodiment, the filter 1041 is composed of at least four filters, and each filter can filter the light projected on the filter, so that the light transmitted through the filter is light of a specific spectrum band.

In this embodiment, the filter switching member 1042 may switch the filters of the optical filter in a program control manner, or may switch the filters of the optical filter in a mechanical switching manner.

For example, the following steps are carried out: referring to fig. 6, another structural schematic diagram of the sample reading device on the basis of the first embodiment is shown, wherein the light source part 100 can generate light of continuous spectrum, the light generated by the second light source generator 103 passes through the filter part, and the light generated by the second light source generator 103 is irradiated onto the sample carried by the sample part 200 through the filter part 104, wherein the light irradiated onto the sample through the filter part is light of specific spectrum, the sample is arranged on the glass slide, the imaging device 300 captures imaging information of the sample under the light irradiation of each spectrum band, and generates a sample image based on the imaging information of the sample under the light irradiation of each spectrum band.

In this embodiment, the second embodiment provides a method of obtaining light of different spectral bands by using a filter member, in which each member is simple and low in cost.

The sample part 200 is used for carrying a sample; in this embodiment, the sample member can hold a sample, or further, the sample member can fix a sample, for example, the sample member may be a stage.

For example, the following steps are carried out: if the sample is blood, the blood sample is placed on a slide and the slide is placed or secured on the sample block.

In this embodiment, the imaging device needs to capture the sample on the sample part, that is, the sample carried on the sample part needs to be able to be placed under the objective lens of the imaging device, and the imaging device can shoot the sample through the objective lens.

However, after the sample is placed or fixed on the sample part, the position where the sample is placed for the first time may not be captured by the imaging device, and in order to conveniently allow the imaging device to capture the sample on the sample part, the sample part needs to be able to adjust the position of the sample, for example, the sample part includes: a sample movement control module for adjusting a position of the sample relative to an imaging device to enable the imaging device to acquire a region of interest of the sample on the sample member.

In this embodiment, the spectral band of light irradiated on the sample is changed, and then the imaging device photographs the sample, so as to obtain sample images of different spectral bands.

An imaging device 300 to sequentially irradiate the sample carried by the sample member with the light of at least four spectral bands generated by the light source member, capture imaging information of the sample under the irradiation of the light of each spectral band, and generate an image of the sample based on the imaging information of the sample under the irradiation of the light of each spectral band;

in this embodiment, light of different bands generated by the light source unit is irradiated on the sample, and the imaging device captures imaging information of the sample under the light of different bands and generates an image of the sample, wherein the imaging device 300 collects the imaging information of the sample under the light of at least four bands, respectively, to obtain images of the sample of different bands.

In this embodiment, the imaging device 300 may be, for example, a wide-spectrum grayscale camera.

And the processor 400 is used for fusing the sample images obtained under the action of at least four spectral bands.

In this embodiment, the sample image is a sample image of a sample taken under at least four spectral bands, and is represented by at least four spectral bands, wherein the color of the sample image of each spectral band mainly highlights the color of the spectral band.

For example, the following steps are carried out: taking a sample image taken at the first spectral band, the sample image predominantly highlighting the color of light of the first spectral band; taking a sample image taken in a second spectral band, the sample image predominantly highlighting the color of light in the second spectral band; taking an image of the sample taken at the third spectral band, the sample image predominantly highlighting the color of light in the third spectral band; an image of the resulting sample is taken at the fourth spectral band, the sample image emphasizing mainly the color of light of the fourth spectral band.

In this embodiment, the sample images obtained under the action of the at least four spectral bands are fused, and the colors of the obtained images are obtained under the adjustment of the colors of the four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced.

In this embodiment, in order to further reduce the color deviation of the sample image, the processor is further configured to: fusing sample images obtained under the action of the at least four spectral bands based on the weight of each spectral band in the at least four spectral bands; for example, the pixels of each sample image are fused according to the weight, so as to obtain a fused sample image.

The weight of each of the at least four spectral bins is determined by:

adjusting weights of at least four spectral bands of a first image of a reference sample such that colors of the first image match colors of a second image of the reference sample, determining a weight for each of the at least four spectral bands; the second image is an image with a color display effect meeting a preset condition, and the first image is an image with a color display effect not meeting the preset condition.

For example, the following steps are carried out: the method comprises the steps of obtaining light of 6 spectra of red, green, blue, magenta, cyan and yellow through a light source part, respectively irradiating the light of the 6 spectra onto a sample, acquiring imaging information of the sample under the irradiation of the light of the 6 spectra through an imaging device to obtain sample images of different spectra, fusing the sample images of the spectra according to preset weight of each spectrum, and specifically fusing pixel values of the sample images of the spectra according to the weight of each spectrum to obtain a fused sample image.

In this embodiment, the preset condition may be set by the user based on the requirement, for example, the preset condition may be related to the degree of color deviation, or the preset condition may be related to the color of the color, for example, the preset condition is that the color deviation of the image is smaller than a preset threshold, or the color of the color meets the requirement of the user.

By way of example: the color deviation degree of the second image is smaller than a preset threshold value, or the color presenting effect of the second image meets the requirements of a user; the color deviation degree of the first image is larger than a preset threshold value, or the color presenting effect of the first image does not meet the requirement of a user.

In this embodiment, when determining the weight of each spectrum, the second image meeting the preset condition is used as the reference object, and in one implementation, the second image is an image capable of presenting the true color of the reference sample, so that when adjusting the color of the image based on the weight of each spectrum determined by using the second image as the reference object, the color of the image is closer to the true color.

In this embodiment, the first image may be an image obtained by fusing a plurality of spectral bands, or the first image is a color image, weights of at least four spectral bands of the first image are continuously adjusted until the color of the first image matches the color of the second image, and the weights of at least four spectral bands that match the color of the first image with the color of the second image are used as an output result. When detecting whether the color of the first image is matched with the color of the second image, if the first image and the second image are not completely consistent, if the color of the first image approaches to the color of the second image, it can be determined that the color of the first image is matched with the color of the second image.

In this embodiment, with the above-mentioned sample reading device, the light source component generates light of at least four spectral bands, and the light of at least four spectral bands sequentially irradiates on the sample carried by the sample component, so that the imaging device can acquire imaging information of the sample respectively irradiated by at least four spectral bands, thereby generating a sample image, and the sample images obtained under the action of at least four spectral bands are fused. Therefore, the colors of the obtained image are obtained under the adjustment of the colors of at least four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced. Moreover, the structure for acquiring the sample image by the sample reading device is simple and easy to realize.

Furthermore, the sample images obtained under the action of at least four spectral bands are fused through the predetermined weight of each spectral band, so that the color accuracy of the fused sample images is further improved, the fused sample images are closer to real colors, or the fused sample images meet the requirements of users better.

Example 2

Referring to fig. 7, a schematic structural diagram of an interpretation device provided in embodiment 2 of the present invention is shown, and in this embodiment, the device includes:

a light source unit 500, a sample unit 600, a first imaging device 700, a second imaging device 800, a processor;

the light source part 500 for generating light irradiated on the sample;

the sample part 600 is used for carrying a sample;

the first imaging device 700 is configured to capture imaging information of the sample on a first optical path, and obtain a first image; the imaging information on the first optical path is generated by the sample under irradiation of continuous spectrum light generated by a light source part;

the second imaging device 800 is configured to capture imaging information of the sample on a second optical path, so as to obtain a second image, where the imaging information on the second optical path is generated by the sample under the action of light of a first target spectrum band, and the light of the first target spectrum band is light of a specific spectrum band;

a processor 900 for fusing the first image and the second image.

As can be seen from the above description, in this embodiment, there are two optical paths, one of which is continuous spectrum light, and the other is specific spectrum light, and in an embodiment, the two optical paths are obtained by the action of the light splitting component, and referring to fig. 8, further includes:

a spectroscopic part 1000 and a filter part 2000;

the light splitting part 1000 is disposed on a third light path, and disperses light having a continuous spectrum generated by the light source part onto the first light path and the second light path;

the filtering component 2000 is disposed on the second light path, and performs filtering processing on the light on the second light path to obtain light of a specific spectrum band under the action of the filtering component.

In this embodiment, the light generated by the light source unit 500 is a continuous spectrum of light. Wherein the continuous spectrum is a complete natural spectrum without the loss of visible spectrum.

The light generated by the light source unit 500 is irradiated onto the sample carried by the sample unit 600 to form a third light path, and the third light path passes through the light splitting unit 1000 to obtain a first light path and a second light path. The second light path is provided with a filtering component 2000, and after the light on the third light path passes through the filtering component 2000, the filtering component filters the passing light, so that the light with a specific spectrum band is obtained. A second imaging device 800 is further disposed on the second optical path, and after the light on the second optical path passes through the optical filter, the second imaging device 800 captures imaging information of the sample on the second optical path, specifically, the second imaging device 800 captures imaging information of the sample under the action of the light of the first target spectrum. Only the first imaging device 700 is disposed on the first optical path, the first imaging device 700 capturing light generated by the sample under illumination by a continuous spectrum of light.

The light of the first target spectrum may be a spectrum meeting the user requirement, for example, if the color spectrum of the first imaging device is the second target spectrum, then the first target spectrum is any spectrum except the second target spectrum. Alternatively, the first target spectrum may be related to the actual color of the sample, and assuming that the color of the sample is purple, the first target spectrum is a spectrum capable of emitting purple light.

In this embodiment, the light splitting member 2000 may be a light splitter or a light splitting prism having a light splitting function, and in addition, the light splitting member may be any device having a light splitting function, which is not limited in this embodiment.

The filtering component 2000 may be any filtering device, such as a filter, or may be another device capable of filtering a continuous spectrum.

However, after the sample is placed or fixed on the sample part, the position where the sample is placed for the first time may not be captured by the imaging device, and in order to conveniently allow the imaging device to capture the sample on the sample part, the sample part needs to be able to adjust the position of the sample, for example, the sample part includes: a sample movement control module to adjust a position of the sample relative to an imaging device to enable the imaging device to acquire a region of interest of the sample on the sample member.

In the present embodiment, the first imaging device is disposed on the first optical path, and captures imaging information of the specimen on the first optical path, and obtains the first image, wherein the first image is obtained by the specimen under the influence of light of a continuous spectrum generated by the light source section. The first imaging device may be, for example, a color camera, and specifically, the first imaging device may be an RGB color camera.

In this embodiment, the second imaging device 800 is disposed on the second light path, the light source component 500 generates light of a continuous spectrum, the generated light of the continuous spectrum is irradiated onto the sample carried by the sample component 600 to form a third light path, the light of the third light path is subjected to a light splitting prism to obtain the first light path and the second light path, the filtering component 2000 is disposed on the second light path, the light of the second light path obtains light of a specific spectrum band under the action of the filtering component 2000, that is, light of a first target spectrum band is obtained, so that the second imaging device can capture imaging information of the sample under the action of the light of the first target spectrum band to obtain a second image. For example, the second imaging device may be a wide spectrum grayscale camera.

It can be known from the above description that the first image is obtained through imaging information of a sample acquired by the first imaging device under irradiation of continuous spectrum light, the obtained first image is a color image, the second image is obtained through imaging information of a sample acquired by the second imaging device under the action of the light of the first target spectrum band, and the first image and the second image are fused through the processor, so that color deviation of the color image is made up, color accuracy is improved, and requirements of users are met better.

In this embodiment, in order to further reduce the color deviation of the sample image, the processor is further configured to: fusing the first image and the second image based on the weight of the first target spectrum section and the weight of the second target spectrum section; the second target spectrum is a plurality of spectrum bands for determining the color of the image shot by the first imaging device, and the first target spectrum is a specific spectrum band;

the method for acquiring the weights of the first target spectrum band and the second target spectrum band comprises the following steps:

In this embodiment, the second target spectrum includes a plurality of spectra that determine the color of the image captured by the first imaging device, the plurality of spectra being any of a plurality of spectra, for example: if the first imaging device is an RGB color camera, the second target spectrum segment includes: red, blue and green, the first target spectrum is any one of the three spectrums except the red, blue and green, for example, the first target spectrum is purple.

For example, the following steps are carried out: the color deviation degree of the fourth image is smaller than a preset threshold, or the color of the fourth image meets the user requirement, and the color deviation degree of the third image is larger than the preset threshold, or the color of the third image does not meet the user requirement.

In this embodiment, the second target spectrum of the third image of the reference sample and the weight of the first target spectrum are continuously adjusted, and the adjustment is stopped if the color of the adjusted third image matches the color of the fourth image of the reference sample, and the weight of the second target spectrum and the weight of the first target spectrum at this time are used as the output result.

In this embodiment, two optical paths are provided, and a first imaging device and a second imaging device are respectively provided on the two optical paths, light on the first optical path is light of a continuous spectrum, light on the second optical path is light of a specific spectrum band, the first imaging device captures imaging information of a sample on the first optical path to obtain a first image, the second imaging device captures imaging information of the sample on the second optical path to obtain a second image, and the first image and the second image are fused by a processor. Therefore, the colors of the color image are adjusted through other spectrums, the color deficiency of the color image is made up, and the color accuracy is improved. In addition, the film reader can simultaneously acquire the sample image under the irradiation of continuous spectrum light and the sample image under the action of specific spectrum light by arranging two light paths, so that the data processing efficiency is improved.

Furthermore, the first image obtained by the first imaging device and the second image obtained by the second imaging device are fused by the predetermined weight of the first target spectrum and the second spectrum, so that the color accuracy of the fused sample image is further improved, the fused sample image is closer to the real color, or the fused sample image better meets the requirements of users.

On the basis of fig. 7, as can be seen from the above description, in the present embodiment, two optical paths are included, the first imaging device captures imaging information of the sample on the first optical path, and the second imaging device captures imaging information of the sample on the second optical path, where the imaging information on the first optical path is generated by the sample under the irradiation of the continuous spectrum of light generated by the light source unit, the imaging information on the second optical path is generated by the sample under the action of the first target spectrum band of light, and the first target spectrum band of light is a specific spectrum band of light.

In another embodiment, the light of the two light paths may be generated by a light source, and in particular, referring to fig. 9, the light source component 500 includes:

a third light source generator 501 for generating light of a continuous spectrum and light of the first target spectrum band;

a light source switching device 502 for controlling the spectrum of the light generated by the third light source generator to switch the light emitted by the third light source generator.

In this embodiment, the third light source generator 501 has the capability of generating continuous spectrum light and specific spectrum light, for example, the third light source generator 501 may be composed of a visible light source generator for generating continuous spectrum light and a laser generator for generating specific spectrum light.

A light source switching means 502 for controlling the spectrum of light generated by the third light source generator, wherein the light source switching means may be a device that controls the spectrum of light generated by the third light source generator by a program or switches the third light source generator to generate a light source by mechanical means.

In this embodiment, the continuous spectrum light and the specific spectrum light generated by the light source unit are respectively irradiated onto the sample carried by the sample unit 600, the continuous spectrum light is irradiated onto the sample to form a first optical path, the first target spectrum light (specific spectrum) is irradiated onto the sample to form a second optical path, the first optical path is provided with the first imaging device, and the second optical path is provided with the second imaging device. The first imaging device 700 captures imaging information of the sample on a first optical path resulting in a first image, and the second imaging device 800 captures imaging information of the sample on a second optical path resulting in a second image. And the processor is used for fusing the first image and the second image.

Further, the processor is further configured to: fusing the first image and the second image based on the weight of the first target spectrum segment and the weight of the second target spectrum segment; the second target spectrum is a plurality of spectrum bands for determining the colors of the image shot by the first imaging device, and the first target spectrum is any one specific spectrum band;

In this embodiment, two optical paths are provided, and a first imaging device and a second imaging device are respectively provided on the two optical paths, light on the first optical path is light of a continuous spectrum, light on the second optical path is light of a specific spectral band, the first imaging device captures imaging information of a sample on the first optical path to obtain a first image, the second imaging device captures imaging information of the sample on the second optical path to obtain a second image, and the first image and the second image are fused by a processor. Therefore, the colors of the color image are adjusted through other spectrums, the color deficiency of the color image is made up, and the color accuracy is improved. And moreover, two light paths are obtained by emitting different lights through the light source, and the structure is simple and easy to realize.

Example 3

Referring to fig. 10, a schematic flow chart of an interpretation method provided in embodiment 3 of the present invention is shown, and in this embodiment, the method includes:

s1001: acquiring imaging information of a sample under the light irradiation of at least four spectral bands in sequence to obtain a plurality of sample images; each sample image is generated based on imaging information of the sample under illumination by light of one spectral band;

in the present embodiment, the light of each spectrum band represents monochromatic light of a different wavelength range. For example, if the sample section produces four spectral bands of light, it is expressed as: the spectrum analyzer comprises a first spectrum band, a second spectrum band, a third spectrum band and a fourth spectrum band, wherein the first spectrum band, the second spectrum band, the third spectrum band and the fourth spectrum band are respectively narrow spectrum light or wide spectrum light with different wavelength ranges. The first, second, third and fourth spectral ranges may be any four different wavelength ranges, and the different wavelength ranges may have partial spectral ranges overlapping or may not overlap at all. However, the light source generated by the light source section is not limited to the light of the above four spectral bands.

In this embodiment, each sample image is obtained by irradiating the sample with light of at least four spectral bands, and specifically, each sample image is obtained based on the sample interpreting device provided in embodiment 1.

S1002: and fusing a plurality of sample images obtained under the action of at least four spectral bands.

In this embodiment, after the sample images obtained under the action of the at least four spectral bands are fused, the colors of the obtained images are obtained under the adjustment of the colors of the four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced.

In this embodiment, in order to further reduce the color deviation of the sample image, the method is further configured to:

the weight of each of the at least four spectral bins is determined by:

adjusting weights of at least four spectral bands of a first image of the reference sample such that colors of the first image match colors of a second image of the reference sample, determining a weight for each of at least four spectral bands; the second image is an image with a color display effect meeting a preset condition, and the first image is an image with a color display effect not meeting the preset condition.

For example, the following steps are carried out: the color deviation degree of the second image is smaller than a preset threshold value, or the color presenting effect of the second image meets the requirements of a user; the color deviation degree of the first image is larger than a preset threshold value, or the color presenting effect of the first image does not meet the requirement of a user.

In this embodiment, based on the weight of each of the at least four spectral bands, the sample images obtained under the action of the at least four spectral bands are fused, and the pixels of each sample image are fused according to the weight, so as to obtain a fused sample image.

In this embodiment, the first image may be an image obtained by fusing a plurality of spectral bands, or the first image is a color image, weights of at least four spectral bands of the first image are continuously adjusted until the color of the first image matches the color of the second image, and the weights of at least four spectral bands that match the color of the first image with the color of the second image are used as an output result. When detecting whether the color of the first image matches with the color of the second image, if it is difficult to achieve that the first image and the second image are completely consistent, if the color of the first image approaches to the color of the second image, it also indicates that the color of the first image matches with the color of the second image.

In this embodiment, the light source component generates light of at least four spectral bands, and the light of at least four spectral bands sequentially irradiates on the sample carried by the sample component, so that the imaging device can acquire imaging information of the sample respectively irradiated by the at least four spectral bands, thereby generating a sample image, and the sample images obtained under the action of the at least four spectral bands are fused. Therefore, the colors of the obtained image are obtained under the adjustment of the colors of at least four spectral bands, so that the color accuracy is improved, and the color deviation is greatly reduced.

Example 4

Referring to fig. 11, a schematic flow chart of a sample interpretation method provided in embodiment 4 of the present invention is shown, and in this embodiment, the method includes:

s1101: acquiring imaging information of a biological sample on a first optical path through first imaging equipment to obtain a first image;

s102: acquiring imaging information of the biological sample on a second optical path through second imaging equipment to obtain a second image;

s103: and fusing the first image and the second image.

In this embodiment, the light of the first target spectrum may be a spectrum meeting the user requirement, and more specifically, if the color spectrum of the first imaging device is determined to be the second target spectrum, the first target spectrum is any spectrum except the second target spectrum. Alternatively, the first target spectrum may be related to the actual color of the sample, and assuming that the color of the sample is purple, the first target spectrum is a spectrum capable of emitting purple light.

The first imaging device may be, for example, a color camera, and specifically, the first imaging device may be an RGB color camera.

Wherein the second imaging device may be a grayscale camera.

In this embodiment, the first image and the second image can be obtained by the specimen reading device disclosed in embodiment 2, and specifically, refer to the disclosure of embodiment 2.

It can be known from the above description that the first image is obtained through imaging information of a sample acquired by the first imaging device under the irradiation of continuous spectrum light, the obtained first image is a color image, the second image is obtained through imaging information of the sample acquired by the second imaging device under the action of the light of the first target spectrum, and the first image and the second image are fused by the processor, so that the color deviation of the color image is compensated, the color accuracy is improved, and the requirements of users are met.

In this embodiment, in order to further reduce the color deviation of the sample image, the method further includes:

fusing the first image and the second image based on the weight of the first target spectrum segment and the weight of the second target spectrum segment; the second target spectrum is a plurality of spectrums which determine the colors of the image shot by the first imaging device, and the first target spectrum is a specific spectrum;

In this embodiment, the second target spectrum includes a plurality of spectrum bands for determining colors of the image captured by the first imaging device, and the plurality of spectrum bands are arbitrary spectrum bands, for example, if the first imaging device is an RGB color camera, the second target spectrum includes: red, blue and green, the first target spectrum is any one of the three spectrums except the red, blue and green, for example, the first target spectrum is purple.

For example, the following steps are carried out: the color deviation degree of the fourth image is smaller than a preset threshold value, or the color of the fourth image meets the user requirement, and the color deviation degree of the third image is larger than the preset threshold value, or the color of the third image does not meet the user requirement.

In this embodiment, the second target spectrum of the third image of the reference sample and the weight of the first target spectrum are continuously adjusted, and the adjustment is stopped when the color of the adjusted third image matches the color of the fourth image of the reference sample, and the weight of the second target spectrum and the weight of the first target spectrum at this time are used as output results.

In this embodiment, two optical paths are provided, and a first imaging device and a second imaging device are respectively provided on the two optical paths, light on the first optical path is light of a continuous spectrum, light on the second optical path is light of a specific spectral band, the first imaging device captures imaging information of a sample on the first optical path to obtain a first image, the second imaging device captures imaging information of the sample on the second optical path to obtain a second image, and the first image and the second image are fused by a processor. Therefore, the colors of the color image are adjusted through other spectrums, the color deficiency of the color image is made up, and the color accuracy is improved.

It should be noted that, in this specification, each embodiment is described in a progressive manner, and each embodiment focuses on differences from other embodiments, and portions that are the same as and similar to each other in each embodiment may be referred to.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A specimen slide reading device is characterized by comprising:

a light source component for generating at least four bands of light encompassing at least the visible light spectral range; a sample part for carrying a sample;

2. The specimen interpreting device as claimed in claim 1, wherein said processor is further configured to:

the weight of each of the at least four spectral bins is determined by:

3. The specimen interpreting device according to claim 1, wherein said light source means comprises: a first light source generator and a light source switching section;

4. The specimen slide reading device according to claim 1, wherein the light source unit comprises: a second light source generator and a filter member;

5. The sample reader device of claim 4, wherein the filter member comprises: an optical filter and an optical filter switching member;

the optical filter switching component is used for controlling the optical filter in the optical filter to switch so as to enable the switched optical filter to be positioned on the light path from the light source component to the sample.

6. The specimen slide interpreting device as claimed in claim 1, wherein said specimen means comprises:

7. A specimen slide reading device, comprising:

a light source part for generating light irradiated on the sample;

a sample part for carrying a sample;

a second imaging device, capturing imaging information of the sample on a second optical path to obtain a second image, wherein the imaging information on the second optical path is generated by the sample under the action of light of a first target spectrum band, and the light of the first target spectrum band is light of a specific spectrum band;

and the processor is used for fusing the first image and the second image.

8. The specimen interpreting device as claimed in claim 7, wherein said processor is further configured to:

9. The specimen slide reading device according to claim 7, wherein the light source means comprises:

10. The specimen slide interpreting device according to claim 7, further comprising:

a light splitting part and a light filtering part;

11. The specimen slide reading apparatus according to claim 7, wherein the first imaging device is a color camera and the second imaging device is a grayscale camera.

12. A sample reading method is characterized by comprising the following steps:

acquiring imaging information of a sample under the light irradiation of at least four spectral bands in sequence to obtain a plurality of sample images; each sample image is generated based on imaging information of the sample under illumination of light of a spectral band, the light of the at least four spectral bands covering at least a visible light spectral range;

13. The method of claim 12, comprising:

the weight of each of the at least four spectral bins is determined by:

14. A sample reading method is characterized by comprising the following steps:

and fusing the first image and the second image.

15. The method of claim 14, further comprising:

fusing the first image and the second image based on the weight of the first target spectrum segment and the weight of the second target spectrum segment; the second target spectrum is a plurality of spectrum bands for determining the color of the image shot by the first imaging device, and the first target spectrum is a specific spectrum band;