CN115895865A - Sequencer control system - Google Patents
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- CN115895865A CN115895865A CN202211668430.1A CN202211668430A CN115895865A CN 115895865 A CN115895865 A CN 115895865A CN 202211668430 A CN202211668430 A CN 202211668430A CN 115895865 A CN115895865 A CN 115895865A
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Abstract
The present disclosure provides a sequencer control system, comprising: the upper computer is used for issuing a first control instruction aiming at the monitoring subsystem to the central control subsystem through the first communication interface and/or issuing a second control instruction aiming at the sequencing subsystem to the central control subsystem through the second communication interface; the central control subsystem is used for acquiring monitoring data acquired by the monitoring subsystem based on the first control instruction when the first control instruction is received; sending the monitoring data to an upper computer through a first communication interface; when receiving the second control command, sending the second control command to the sequencing subsystem, receiving communication control data returned by the sequencing subsystem, and sending the communication control data to the upper computer through the second communication interface; the monitoring subsystem is used for monitoring at least one sequencing index in the sequencing process to obtain monitoring data; and the sequencing subsystem is used for performing sequencing operation based on the second control instruction to generate communication control data.
Description
Technical Field
The disclosure relates to the technical field of gene detection, in particular to a sequencer control system.
Background
In the field of genetic testing technology, a sequencer control system is generally used to detect a genomic sequence. The sequencer control system is a complex system comprising a plurality of components such as machinery, electronics, a liquid path, optics, image processing and the like; generally, a sequencer control system monitors the working state of each component in the working process while controlling each component to work, so as to ensure that each component can work stably. Therefore, a need exists for a reliable and stable sequencer control system.
Disclosure of Invention
In view of this, the present disclosure at least provides a sequencer control system to improve the stability of the sequencer control system through the orderly control of the upper computer to the lower computer.
In a first aspect, the present disclosure provides a sequencer control system, comprising: the system comprises an upper computer, a central control subsystem, a monitoring subsystem and a sequencing subsystem; the central control subsystem is respectively connected with the upper computer, the monitoring subsystem and the sequencing subsystem;
the upper computer is used for issuing a first control instruction aiming at the monitoring subsystem to the central control subsystem through a first communication interface and/or issuing a second control instruction aiming at the sequencing subsystem to the central control subsystem through a second communication interface;
the central control subsystem is used for acquiring monitoring data acquired by the monitoring subsystem based on the first control instruction when the first control instruction is received; sending the monitoring data to the upper computer through the first communication interface; when the second control instruction is received, the second control instruction is issued to the sequencing subsystem, communication control data which are generated after sequencing operation is carried out based on the second control instruction and returned by the sequencing subsystem are received, and the communication control data are sent to the upper computer through the second communication interface;
the monitoring subsystem is used for monitoring at least one sequencing index in the sequencing process to obtain monitoring data;
and the sequencing subsystem is used for carrying out sequencing operation based on the second control instruction and generating communication control data.
In consideration of the fact that the amount of data generated in the control process is large, in order to ensure that the monitoring subsystem and the sequencing subsystem do not interfere with each other and the stability of an instrument is guaranteed, the upper computer provided by the embodiment of the disclosure can issue a first control instruction for the monitoring subsystem to the central control subsystem through the first communication interface, and/or issue a second control instruction for the sequencing subsystem to the central control subsystem through the second communication interface; meanwhile, the monitoring subsystem and the sequencing subsystem are only connected with the central control subsystem, so that the central control subsystem can uniformly control the subsystems after receiving a control instruction sent by an upper computer, so that the subsystems can work orderly, and the stability of the sequencer control system is high.
In one possible embodiment, the monitoring subsystem comprises at least one of the following sensors: the system comprises a sensor for monitoring the state of a reagent box door, a sensor for monitoring the state of a waste liquid buffer box door, a sensor for monitoring whether a chip is placed, a sensor for monitoring whether a chip clamp is clamped, a sensor for monitoring whether a waste liquid buffer box is placed in place, a sensor for monitoring whether a kit is placed in place, a sensor for monitoring whether the kit enters a sequencing position, a bubble sensor, a flow velocity sensor, a sensor for monitoring the height of liquid in the waste liquid buffer box, a pressure sensor and a sensor for monitoring the state information of the chip, the kit and the waste liquid buffer box;
each sensor in the monitoring subsystem is respectively connected with the central control subsystem through an interface.
Here, in the case that the monitoring subsystem includes at least one sensor, the sequencing index in the sequencing process can be monitored in real time through the at least one sensor, so as to ensure the stable operation of the sequencer control system; meanwhile, each sensor is connected with the central control subsystem through the interfaces respectively, so that the sensors connected with other interfaces can work normally when any interface breaks down, and the stability of a sequencer control system is improved.
In a possible embodiment, when acquiring the monitoring data acquired by the monitoring subsystem based on the first control 5 instruction, the central control subsystem is configured to:
analyzing the first control instruction to obtain first instruction information; the first instruction information is used for indicating target monitoring data of a required type and a target sensor for collecting the target monitoring data;
coding the first instruction information to generate a first target instruction;
0 controlling a target interface connected with the target sensor based on the first target instruction, and connecting
And receiving target monitoring data sent by the target sensor.
In one possible embodiment, the sequencing subsystem comprises at least one of the following detection modules:
the device comprises a temperature control module, an injection pump, a distribution valve, a horizontal direction control module, a vertical direction control module, a Light Emitting Diode (LED) module and a Laser Diode (LD) module;
5 each detection module in the sequencing subsystem is respectively connected with the central control subsystem through an interface
And (4) connecting the systems.
In the case where the sequencing subsystem includes at least one detection module, each detection module is connected to the central control subsystem via a respective interface, it is possible to ensure that, in the event of a failure of any interface,
the detection module connected with other interfaces can work normally, and the stability of the sequencer control system 0 is improved.
In a possible embodiment, when the central control subsystem issues the second control instruction to the sequencing subsystem, the central control subsystem is configured to:
analyzing the second control instruction to obtain second instruction information; the second instruction information is used for indicating a target detection module to be controlled;
5, encoding the second instruction information to generate a second target instruction;
and issuing the second target instruction to the target detection module through a target interface connected with the target detection module.
In a possible embodiment, when the target detection module includes a distribution valve, the distribution valve is configured to, in response to receiving the second target instruction, control a valve of the distribution valve to rotate according to a target rotation direction indicated by the second target instruction until reaching a target position corresponding to target position information indicated by the second target instruction;
when the target detection module comprises a syringe pump, the syringe pump is used for sucking the target reagent or pushing the target reagent based on at least one of the reagent type, the speed information and the volume information indicated by the second target instruction in response to receiving the second target instruction.
In a possible embodiment, when the object detection module comprises a horizontal direction control module, the horizontal direction control module comprises a horizontal platform and a control unit;
and the control unit is used for responding to the received second target instruction and controlling the horizontal platform to move according to the moving direction and/or the moving distance indicated by the second target instruction.
In one possible embodiment, the sequencing subsystem further comprises: the kit moving module and the waste liquid box motor are arranged on the kit; the reagent box moving module and the waste liquid box motor are respectively connected with the horizontal direction control module;
when the target detection module comprises a horizontal direction control module and a reagent box moving module, the horizontal direction control module is further used for: in response to receiving the second target instruction, controlling the reagent box moving module to move according to the moving direction and/or the moving distance indicated by the second target instruction;
when the target detection module includes horizontal direction control module and waste liquid box motor, horizontal direction control module still is used for: and controlling the waste liquid box motor to rotate according to the moving direction and/or the moving distance indicated by the second target instruction in response to the received second target instruction so as to drive the waste liquid box to move.
In a possible implementation manner, when the target detection module includes an LD module, the LD module is configured to control an LD light source in the LD module to emit laser light in response to receiving the second target instruction.
In a possible embodiment, the sequencing subsystem further comprises a sequencer camera, and the sequencer camera is connected with the vertical direction control module;
when the target detection module comprises a vertical direction control module, the vertical direction control module is configured to, in response to receiving the second target instruction, perform at least one of:
controlling a motor of the sequencer camera to move so as to drive a lens of the sequencer camera to move to a target position corresponding to the second target instruction indication target position information; wherein the target location comprises: the target location includes: a position where the accuracy of the sequencer camera matches a preset accuracy, and/or a position where the sequencer camera focuses on;
and controlling the power supply of the vertical direction control module to be disconnected.
In a possible embodiment, when the target detection module includes an LED module, the LED module is configured to control an LED light source in the LED module to emit a light beam based on illumination intensity information and/or color information indicated by the second target instruction in response to receiving the second target instruction.
In a possible implementation, when the target detection module includes a temperature control module, the temperature control module is configured to adjust the ambient temperature to a target temperature indicated by the second target instruction in response to receiving the second target instruction.
In order to make the aforementioned objects, features and advantages of the present disclosure more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings required for use in the embodiments will be briefly described below, and the drawings herein incorporated in and forming a part of the specification illustrate embodiments consistent with the present disclosure and, together with the description, serve to explain the technical solutions of the present disclosure. It is appreciated that the following drawings depict only certain embodiments of the disclosure and are therefore not to be considered limiting of its scope, for those skilled in the art will be able to derive additional related drawings therefrom without the benefit of the inventive faculty.
FIG. 1 illustrates an architectural diagram of a sequencer control system provided by an embodiment of the present disclosure;
FIG. 2 is a schematic diagram illustrating an architecture of a monitoring subsystem in a sequencer control system according to an embodiment of the disclosure;
FIG. 3 is a schematic diagram illustrating an architecture of a sequencing subsystem in a sequencer control system according to an embodiment of the disclosure;
fig. 4 is a schematic diagram illustrating an architecture of another sequencing subsystem in a sequencer control system according to an embodiment of the disclosure.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are only a part of the embodiments of the present disclosure, not all of the embodiments. The components of the embodiments of the present disclosure, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present disclosure, presented in the figures, is not intended to limit the scope of the claimed disclosure, but is merely representative of selected embodiments of the disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the disclosure without making creative efforts, shall fall within the protection scope of the disclosure.
In the field of genetic testing technology, a sequencer control system is generally used to detect a genomic sequence. The sequencer control system is a complex system comprising a plurality of components such as machinery, electronics, a liquid path, optics, image processing and the like; usually, the host computer in the sequencer control system can monitor the operating condition of each subsystem in the course of the work when controlling each subsystem to carry out work to ensure that sequencer control system can stable work.
Generally, in a sequencer control system, an upper computer generally issues an instruction for controlling each subsystem to work and an instruction for acquiring the working state of each subsystem through only one communication interface, and the communication mode can cause the coordination of each subsystem to be disordered and reduce the stability of the sequencer control system. To alleviate the above problems, embodiments of the present disclosure provide a sequencer control system.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures.
Referring to fig. 1, a schematic diagram of a sequencer control system according to an embodiment of the present disclosure is shown, where the sequencer control system includes: the system comprises an upper computer 11, a central control subsystem 12, a monitoring subsystem 13 and a sequencing subsystem 14; the central control subsystem 12 is connected with the upper computer 11, the monitoring subsystem 13 and the sequencing subsystem 14 respectively.
And the upper computer 11 is used for issuing a first control instruction aiming at the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface and/or issuing a second control instruction aiming at the sequencing subsystem 14 to the central control subsystem 12 through the second communication interface.
The central control subsystem 12 is configured to, when receiving the first control instruction, obtain monitoring data acquired by the monitoring subsystem 13 based on the first control instruction; sending the monitoring data to the upper computer 11 through a first communication interface; and when receiving the second control instruction, sending the second control instruction to the sequencing subsystem 14, receiving communication control data, which is returned by the sequencing subsystem 14 and generated after sequencing operation is performed based on the second control instruction, and sending the communication control data to the upper computer 11 through the second communication interface.
And the monitoring subsystem 13 is used for monitoring at least one sequencing index in the sequencing process to obtain monitoring data.
And the sequencing subsystem 14 is used for performing sequencing operation based on the second control instruction and generating communication control data.
When in implementation, the sequencer control system may include an upper computer 11, a central control subsystem 12, a monitoring subsystem 13 and a sequencing subsystem 14; the upper computer 11 is connected with the central control subsystem 12, and the central control subsystem 12 is respectively connected with the monitoring subsystem 13 and the sequencing subsystem 14. Here, the upper Computer 11 may be a Personal Computer (PC), a main Computer, or the like that can directly issue a manipulation command.
In specific implementation, the upper computer 11 may issue a first control instruction for the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface; or, the upper computer 11 may also issue a second control instruction for the sequencing subsystem 14 to the central control subsystem 12 through the second communication interface; or, the upper computer 11 may also issue a first control instruction for the monitoring subsystem 13 to the central control subsystem 12 through the first communication interface, and issue a second control instruction for the sequencing subsystem 14 to the central control subsystem 12 through the second communication interface. And the first communication interface is not communicated with the second communication interface.
The central control subsystem 12 may issue the second control instruction to the sequencing subsystem 14 when receiving the second control instruction; the sequencing subsystem 14 may perform a sequencing operation based on the second control instruction, generate and return communication control data; furthermore, the central control subsystem 12 may receive communication control data, which is returned by the sequencing subsystem 14 and generated after the sequencing operation is performed based on the second control instruction, and send the communication control data to the upper computer 11 through the second communication interface.
The monitoring subsystem 13 can monitor at least one sequencing index in the sequencing process to obtain monitoring data; for example, the sequencing index may include the presence or absence of a sample chip to be detected, the level of waste liquid, the flow rate of liquid, and the like. The central control subsystem 12 may also obtain monitoring data acquired by the monitoring subsystem 13 based on the first control instruction when receiving the first control instruction; and sends the monitoring data to the upper computer 11 through the first communication interface.
In particular, the central control subsystem 12 may further include an indicator light for indicating the operating status of each subsystem; for example, red and yellow indicator lamps can be included, and the red indicator lamp can be controlled to be turned on or be controlled to flash when the subsystem fails; when the subsystem works normally, the green indicator light can be controlled to be on, or the green indicator light can be controlled to flash.
In consideration of the fact that the data volume generated in the gene sequencing process is large, in order to ensure that the monitoring subsystem and the sequencing subsystem do not interfere with each other and the stability of an instrument is guaranteed, the upper computer provided by the embodiment of the disclosure issues a first control instruction for the monitoring subsystem to the central control subsystem through the first communication interface, and/or issues a second control instruction for the sequencing subsystem to the central control subsystem through the second communication interface; meanwhile, the monitoring subsystem and the sequencing subsystem are only connected with the central control subsystem, so that the central control subsystem can uniformly control the subsystems after receiving a control instruction sent by an upper computer, so that the subsystems can work orderly, and the stability of the sequencer control system is high.
In one possible embodiment, the monitoring subsystem 13 includes at least one of the following sensors: the system comprises a sensor for monitoring the state of a reagent box door, a sensor for monitoring the state of a waste liquid buffer box door, a sensor for monitoring whether a chip is placed in, a sensor for monitoring whether a chip clamp is clamped, a sensor for monitoring whether a waste liquid buffer box is placed in place, a sensor for monitoring whether a reagent box enters a sequencing position, a bubble sensor, a flow velocity sensor, a sensor for monitoring the liquid height in the waste liquid buffer box, a pressure sensor and a sensor for monitoring the state information of the chip, the reagent box and the waste liquid buffer box; each sensor in the monitoring subsystem 13 is separately interfaced to the central control subsystem 12.
In practice, the monitoring subsystem 13 may include at least one sensor, for example, as shown in fig. 2, a first sensor 131 for monitoring the status of the cassette door, the first sensor 131 may be interfaced with the central control subsystem 12; specifically, it is possible to monitor whether the cartridge door is in an open state.
A second sensor 132 for monitoring the status of the waste buffer cassette door may also be included, the second sensor 132 being interfaced with the central control subsystem 12; specifically, whether the waste buffer box door is in an open state or not can be monitored; if in the open state, the buffer can be accessed or the waste can be discarded into a waste cartridge.
A third sensor 133 for monitoring whether a chip is inserted may also be included, and the third sensor 133 may be interfaced with the central control subsystem 12.
A fourth sensor 134 may also be included for monitoring whether the chip holder is clamped, and the fourth sensor 134 may be interfaced with the central control subsystem 12.
A fifth sensor 135 may also be included for monitoring whether the waste buffer cartridge is in place, and the fifth sensor 135 may be interfaced with the central control subsystem 12.
A sixth sensor 136 may also be included for monitoring the placement of the reagent cartridge, and the sixth sensor 136 may be interfaced with the central control subsystem 12.
A seventh sensor 137 may also be included for monitoring the entry of the cartridge into the sequencing site, and the seventh sensor 137 may be interfaced with the central control subsystem 12.
A bubble sensor 138 may also be included, the bubble sensor 138 may be interfaced with the central control subsystem 12; specifically, the volume and number of bubbles in the fluid path may be monitored.
A flow rate sensor 139 may also be included, and the flow rate sensor 139 may be interfaced with the central control subsystem 12; in particular, the flow rate of the liquid in the liquid path may be monitored.
An eighth sensor 1310 for monitoring the level of liquid in the waste buffer cartridge may also be included, and the eighth sensor 1310 may be interfaced with the central control subsystem 12.
A pressure sensor 1311 may also be included, the pressure sensor 1311 may be interfaced with the central control subsystem 12; specifically, the pressure state of the liquid path can be monitored, and then whether the liquid path has liquid leakage, blockage and the like can be judged.
A ninth sensor 1312 used for monitoring the state information of the chip, the reagent kit and the waste liquid buffer box can be further included, the ninth sensor 1312 can be a radio frequency card reader and the like, and can be connected with the central control subsystem 12 through an interface; specifically, the state information may include whether the chip is damaged, that is, whether the chip information can be normally collected, chip model information, chip flux information, and the like; the kit can also comprise type information, model information, whether the kit is used, production date, expiration date and the like; and the model information of the waste liquid buffer box, whether the waste liquid buffer box is used, the production date, the effective period and the like can also be included.
In specific implementation, the system can further comprise a temperature sensor, a humidity sensor and the like, can be used for monitoring the temperature and the humidity of the sequencing environment, and can be connected with the central control subsystem 12 through the reserved interface 1.
Here, in the case that the monitoring subsystem includes at least one sensor, the sequencing index in the sequencing process can be monitored in real time through the at least one sensor, so as to ensure the stable operation of the sequencer control system; meanwhile, each sensor is connected with the central control subsystem 12 through an interface respectively, so that when any interface breaks down, the sensors connected with other interfaces can work normally, and the stability of the sequencer control system is improved.
In a possible embodiment, the central control subsystem 12, when acquiring the monitoring data acquired by the monitoring subsystem 13 based on the first control instruction, is configured to:
analyzing the first control instruction to obtain first instruction information; the first instruction information is used for indicating target monitoring data of a required type and a target sensor for collecting the target monitoring data.
And coding the first instruction information to generate a first target instruction.
And based on the first target instruction, controlling a target interface connected with the target sensor and receiving target monitoring data sent by the target sensor.
In implementation, after receiving the first control instruction, the central control subsystem 12 may analyze the first control instruction to obtain first instruction information; specifically, the instruction comprises an information bit and a check bit, and can be used for decoding the first control instruction, and checking whether the instruction information has errors and is modified through the check bit to obtain first instruction information; the first instruction information can be used for indicating target monitoring data of a required type and a target sensor for collecting the target monitoring data; for example, if the upper computer 11 needs to monitor the flow rate of the liquid in the liquid path, the first instruction information may indicate monitoring data of the flow rate of the liquid and a flow rate sensor for acquiring the flow rate of the liquid. Further, the central control subsystem 12 may encode the first instruction information to generate a first target instruction, and may control a target interface connected to the target sensor based on the first target instruction to receive target monitoring data sent by the target sensor; for example, if the interface is in a high state, such as 1, data may be received; if the interface is in a low level state, if the interface is 0, the data is not received; the central control subsystem 12 may control a target interface connected to the target sensor to be in a high level state based on the first target instruction, and then receive target monitoring data sent by the target sensor.
In one possible embodiment, referring to FIG. 3, the sequencing subsystem 14 includes at least one of the following detection modules: a temperature control module 141, a syringe pump 142, a distribution valve 143, a horizontal direction control module 144, a vertical direction control module 145, a light-emitting diode (LED) module 146, and a Laser Diode (LD) module 147; each detection module in the sequencing subsystem 14 is connected to the central control subsystem 12 through an interface.
In practice, the sequencing subsystem 14 may include at least one monitoring module, for example, as shown in fig. 3, including a temperature control module 141, a syringe pump 142, a dispensing valve 143, a horizontal direction control module 144, a vertical direction control module 145, an LED module 146, and an LD module 147; the temperature control module 141 can be connected to the central control subsystem 12 through an interface; the syringe pump 142 may be interfaced with the central control subsystem 12; the distribution valve 143 may be interfaced with the central control subsystem 12; the horizontal direction control module 144 may interface with the central control subsystem 12; the vertical direction control module 145 may be connected to the central control subsystem 12 via an interface; the LED module 146 may be interfaced with the central control subsystem 12; the LD module 147 may interface with the central control subsystem 12.
When the system is implemented, other required detection modules can be further included, and the other required detection modules can be connected with the central control subsystem 12 through the reserved interface 2.
Here, under the condition that the sequencing subsystem comprises at least one detection module, each detection module is respectively connected with the central control subsystem through an interface, so that when any interface breaks down, the detection modules connected with other interfaces can work normally, and the stability of the sequencer control system is improved.
In one possible embodiment, the central control subsystem 12, when issuing the second control command to the sequencing subsystem 14, is configured to:
analyzing the second control instruction to obtain second instruction information; and the second instruction information is used for indicating the target detection module to be controlled.
And coding the second instruction information to generate a second target instruction.
And issuing the second target instruction to the target detection module through a target interface connected with the target detection module.
In implementation, after receiving the second control instruction, the central control subsystem 12 may analyze the second control instruction to obtain second instruction information; specifically, the instruction includes an information bit and a check bit, and the second control instruction can be decoded, and whether the instruction information has errors or not is checked and modified through the check bit, so that second instruction information is obtained; the second instruction information may be used to indicate a target detection module to be controlled. Further, the central control subsystem 12 may provide second command information
And line coding is carried out, a second target instruction is generated, and the second target instruction can be issued to the target detection module through a target interface connected with the target detection module 5.
In a possible embodiment, when the target detection module includes the distribution valve 143, the distribution valve 143 is configured to control, in response to receiving the second target instruction, a valve of the distribution valve 143 to rotate according to a target rotation direction indicated by the second target instruction until reaching a target position corresponding to the target position information indicated by the second target instruction.
0 when the object detection module includes syringe pump 142, for responding to receiving the second signal
And a second target instruction for drawing the target reagent or pushing out the target reagent based on at least one of the reagent type, the speed information, and the volume information indicated by the second target instruction.
In practice, where the object detection module includes the dispensing valve 143, the dispensing valve 143 may be used to respond
In response to receiving the second target command, controlling the valve of the distribution valve 143 to rotate according to the target rotation direction indicated by the second target command 5, for example, to rotate clockwise or to rotate counterclockwise;
until the target position corresponding to the target position information indicated by the second target instruction is reached. For example, if the second target command indicates that the target rotation direction is clockwise and the target position information is the position of the valve port 2; the valve controlling the distribution valve 143 rotates clockwise until reaching the position of the valve port 2. In particular to
In implementation, if the second target command does not indicate the target rotation direction, 0 rotation is performed according to the preset rotation direction.
Where the target detection module includes a syringe pump 142, the syringe pump 142 may be configured to aspirate or expel the target reagent based on at least one of the reagent type, the speed information, and the volume information indicated by the second target instruction in response to receiving the second target instruction.
In specific implementation, if the second target instruction does not indicate speed information or volume information, the target reagent is sucked or pushed out at a preset speed or a preset volume of 5. If the second target instruction does not indicate a test
And the reagent type is used for sucking or pushing out a target reagent with a preset reagent type, or sucking or pushing out a target reagent matched with the last reagent type. Wherein, the preset reagent type, the preset speed and the preset volume can be set according to the actual situation.
Illustratively, when the second target instruction indicates to aspirate reagent of reagent type a, syringe pump 142 may aspirate a preset volume of reagent a at a preset speed; alternatively, when the second target command indicates that reagent type B reagent is to be expelled at a rate of 0.2 milliliters per second, syringe pump 142 may expel a preset volume of reagent B at a rate of 0.2 milliliters per second.
In one possible embodiment, referring to fig. 4, when the object detection module includes the horizontal direction control module 144, the horizontal direction control module 144 includes a horizontal stage 1441 and a control unit 1442.
A control unit 1442, configured to, in response to receiving the second target instruction, control the horizontal platform 1441 to move according to the moving direction and/or the moving distance indicated by the second target instruction.
In practice, when the object detection module includes the horizontal direction control module 144, the horizontal direction control module 144 may include a horizontal platform 1441 and a control unit 1442; horizontal platform 1441 may be used to carry a sample chip; the control unit 1442 may be configured to, in response to receiving the second target instruction, control the horizontal platform 1441 to move according to the moving direction and/or the moving distance indicated by the second target instruction, so as to adjust the position of the sample chip photographed by the sequencer camera in the sequencing subsystem 14; wherein the moving direction may include forward, backward, leftward, and rightward.
In specific implementation, if the second target instruction does not indicate the moving direction or the moving distance, the second target instruction moves according to the preset moving direction or the preset distance. For example, if the moving direction indicated by the second target instruction is moving to the left, the horizontal platform 1441 is controlled to move to the left by a preset distance; if the moving distance indicated by the second target instruction is 5 cm, the horizontal stage 1441 is controlled to move 5 cm in the preset direction. The preset direction and the preset distance can be set according to actual conditions.
In one possible embodiment, as shown in fig. 4, the sequencing subsystem 14 further comprises: a reagent cartridge moving module 148, a waste liquid cartridge motor 149; the reagent cartridge moving module 148 and the waste cartridge motor 149 are connected to the horizontal direction control module 144, respectively.
When the target detection module comprises the horizontal direction control module 144 and the reagent cartridge moving module 148, the horizontal direction control module 144 is further configured to: in response to receiving the second target instruction, the reagent cartridge moving module 148 is controlled to move according to the moving direction and/or the moving distance indicated by the second target instruction.
When the target detection module includes the horizontal direction control module 144 and the waste bin motor 149, the horizontal direction control module 144 is further configured to: in response to receiving the second target instruction, the waste liquid box motor 149 is controlled to rotate according to the moving direction and/or the moving distance indicated by the second target instruction, so as to drive the waste liquid box to move.
In practice, the sequencing subsystem 14 may further include a reagent cartridge moving module 148 and a waste cartridge motor 149; the reagent cartridge moving module 148 and the waste liquid cartridge motor 149 are connected to the horizontal direction control module 144, respectively.
When the target detection module includes the horizontal direction control module 144 and the reagent cartridge moving module 148, the horizontal direction control module 144 may be further configured to control the reagent cartridge moving module 148 to move according to the moving direction and/or the moving distance indicated by the second target instruction in response to receiving the second target instruction. Wherein, the moving direction may include forward, backward, leftward, rightward, upward, downward; movement in the horizontal direction may change the position of the reagent cartridge so that the syringe pump 142 may aspirate reagents of different reagent types; movement in the vertical direction may puncture the reagent sealing cap by the syringe pump 142.
In specific implementation, if the second target instruction does not indicate the moving direction or the moving distance, the second target instruction moves according to the preset moving direction or the preset distance. For example, if the moving direction indicated by the second target instruction is moving upward, the reagent cartridge moving module 148 is controlled to move upward by a preset distance; if the moving distance indicated by the second target instruction is 5 cm, the reagent cartridge moving module 148 is controlled to move 5 cm in the preset direction. The preset direction and the preset distance can be set according to actual conditions.
When the target detection module includes the horizontal direction control module 144 and the waste liquid box motor 149, the horizontal direction control module 144 may be further configured to control the waste liquid box motor 149 to rotate according to the moving direction and/or the moving distance indicated by the second target instruction in response to receiving the second target instruction, so as to drive the waste liquid box to move. Wherein the moving direction may include up and down. In specific implementation, if the second target instruction does not indicate the moving direction or the moving distance, the second target instruction moves according to the preset moving direction or the preset distance.
In one possible embodiment, when the target detection module includes the LD module 147, the LD module 147 is configured to control the LD light source in the LD module 147 to emit laser light in response to receiving the second target instruction.
In practice, when the target detection module includes the LD module 147, the LD module 147 may control the LD light source in the LD module 147 to emit laser light to adjust the focal length of the sequencer camera in the sequencing subsystem 14 to increase the reference point in response to receiving the second target instruction, so as to increase the speed of the sequencer camera focusing.
In one possible embodiment, shown in FIG. 4, the sequencing subsystem 14 further includes a sequencer camera 1410, and the sequencer camera 1410 is coupled to the vertical control module 145.
When the target detection module comprises the vertical direction control module 145, the vertical direction control module 145 is configured to, in response to receiving the second target instruction, perform at least one of:
the first operation controls a motor of the sequencer camera 1410 to move so as to drive a lens of the sequencer camera 1410 to move to a target position corresponding to the second target instruction indication target position information; wherein the target location comprises: a location where the sequencer camera's precision matches the preset precision, and/or a location where the sequencer camera focuses on.
The second operation controls the power of the vertical direction control module 145 to be turned off.
In practice, the sequencing subsystem 14 may further include a sequencer camera 1410, the sequencer camera 1410 being coupled to the vertical orientation control module 145. When the target detection module includes the vertical direction control module 145, the vertical direction control module 145 may be configured to control the motor of the sequencer camera 1410 to rotate in response to receiving the second target instruction, so as to drive the lens of the sequencer camera 1410 to move to the target position corresponding to the second target instruction indication target position information.
For example, in order to ensure that the accuracy of the sequencer camera 1410 is high, after the sequencer camera 1410 is powered on, the lens of the sequencer camera 1410 needs to be moved to a target position; at this time, the target position is a zero point position of the lens of the sequencer camera 1410, that is, when the lens of the sequencer camera 1410 is moved to the target position, the accuracy of the sequencer camera 1410 matches the preset accuracy; alternatively, when the sequencer camera 1410 is focused, the lens of the sequencer camera 1410 needs to be moved to the target position; at this time, the target position is a position at which the sequencer camera 1410 is focused, that is, when the lens of the sequencer camera 1410 is moved to the target position, the image taken by the sequencer camera 1410 is clearer.
In specific implementation, the vertical direction control module 145 may be further configured to disconnect the power supply of the vertical direction control module 145 in response to receiving the second target instruction, so as to reduce the loss of the vertical direction control module 145, and meanwhile, if a lens of the sequencer camera 1410 has a movement error, the operation stability of the instrument may be improved by disconnecting the power supply of the vertical direction control module 145.
In a possible embodiment, when the target detection module includes the LED module 146, the LED module 146 is configured to control the LED light source in the LED module 146 to emit the light beam based on the illumination intensity information and/or the color information indicated by the second target instruction in response to receiving the second target instruction.
In practice, when the target detection module includes the LED module 146, the LED module 146 may control the LED light source in the LED module 146 to emit a light beam in response to receiving the second target instruction, and may excite the fluorescence of different markers in the reacted reagent, so that the sequencer camera 1410 in the sequencing subsystem 14 may obtain image information more easily.
In one possible embodiment, when the target detection module includes the temperature control module 141, the temperature control module 141 is configured to adjust the ambient temperature to the target temperature indicated by the second target instruction in response to receiving the second target instruction.
In practice, where the target detection module includes the temperature control module 141, the temperature control module 141 may be configured to adjust the ambient temperature to the target temperature indicated by the second target command in response to receiving the second target command, so as to provide the appropriate temperature for the reagent reaction.
In the several embodiments provided in the present disclosure, it should be understood that the disclosed system may be implemented in other ways. The above-described system embodiments are merely illustrative, and for example, the division of the units is only one logical functional division, and there may be other divisions in actual implementation, and for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed coupling or direct coupling or communication connection between each other may be through some communication interfaces, indirect coupling or communication connection between devices or units, and may be in an electrical, mechanical or other form.
The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.
In addition, functional units in the embodiments of the present disclosure may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit.
The above are only specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of changes or substitutions within the technical scope of the present disclosure, and shall be covered by the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (10)
1. A sequencer control system, comprising: the system comprises an upper computer, a central control subsystem, a monitoring subsystem and a sequencing subsystem; the central control subsystem is respectively connected with the upper computer, the monitoring subsystem and the sequencing subsystem;
the upper computer is used for issuing a first control instruction aiming at the monitoring subsystem to the central control subsystem through a first communication interface and/or issuing a second control instruction aiming at the sequencing subsystem to the central control subsystem through a second communication interface;
the central control subsystem is used for acquiring monitoring data acquired by the monitoring subsystem based on the first control instruction when the first control instruction is received; sending the monitoring data to the upper computer through the first communication interface; when the second control instruction is received, the second control instruction is issued to the sequencing subsystem, communication control data which are returned by the sequencing subsystem and generated after sequencing operation is carried out based on the second control instruction are received, and the communication control data are sent to the upper computer through the second communication interface;
the monitoring subsystem is used for monitoring at least one sequencing index in the sequencing process to obtain monitoring data;
and the sequencing subsystem is used for carrying out sequencing operation based on the second control instruction and generating communication control data.
2. The sequencer control system of claim 1, wherein the monitoring subsystem includes at least one of the following sensors: the system comprises a sensor for monitoring the state of a reagent box door, a sensor for monitoring the state of a waste liquid buffer box door, a sensor for monitoring whether a chip is placed, a sensor for monitoring whether a chip clamp is clamped, a sensor for monitoring whether a waste liquid buffer box is placed in place, a sensor for monitoring whether a kit is placed in place, a sensor for monitoring whether the kit enters a sequencing position, a bubble sensor, a flow velocity sensor, a sensor for monitoring the height of liquid in the waste liquid buffer box, a pressure sensor and a sensor for monitoring the state information of the chip, the kit and the waste liquid buffer box;
each sensor in the monitoring subsystem is respectively connected with the central control subsystem through an interface.
3. The sequencer control system of claim 2, wherein the central control subsystem, when acquiring the monitoring data collected by the monitoring subsystem based on the first control command, is configured to:
analyzing the first control instruction to obtain first instruction information; the first instruction information is used for indicating target monitoring data of a required type and a target sensor for collecting the target monitoring data;
coding the first instruction information to generate a first target instruction;
and controlling a target interface connected with the target sensor based on the first target instruction, and receiving target monitoring data sent by the target sensor.
4. The sequencer control system of claim 1, wherein the sequencing subsystem comprises at least one of the following detection modules: the device comprises a temperature control module, an injection pump, a distribution valve, a horizontal direction control module, a vertical direction control module, a Light Emitting Diode (LED) module and a Laser Diode (LD) module;
and each detection module in the sequencing subsystem is respectively connected with the central control subsystem through an interface.
5. The sequencer control system of claim 4, wherein the central control subsystem, upon issuing the second control instructions to the sequencing subsystem, is configured to:
analyzing the second control instruction to obtain second instruction information; the second instruction information is used for indicating a target detection module to be controlled;
coding the second instruction information to generate a second target instruction;
and issuing the second target instruction to the target detection module through a target interface connected with the target detection module.
6. The sequencer control system according to claim 4, wherein when the target detection module comprises a distribution valve, the distribution valve is configured to, in response to receiving the second target command, control a valve of the distribution valve to rotate in a target rotation direction indicated by the second target command until reaching a target position corresponding to the target position information indicated by the second target command;
when the target detection module comprises a syringe pump, the syringe pump is used for sucking the target reagent or pushing the target reagent based on at least one of the reagent type, the speed information and the volume information indicated by the second target instruction in response to receiving the second target instruction.
7. The sequencer control system of claim 4, wherein when the target detection module comprises a horizontal orientation control module, the horizontal orientation control module comprises a horizontal platform and a control unit;
and the control unit is used for responding to the received second target instruction and controlling the horizontal platform to move according to the moving direction and/or the moving distance indicated by the second target instruction.
8. The sequencer control system of claim 4, wherein the sequencing subsystem further comprises: the kit moving module and the waste liquid box motor are arranged on the kit; the reagent box moving module and the waste liquid box motor are respectively connected with the horizontal direction control module;
when the target detection module comprises a horizontal direction control module and a reagent box moving module, the horizontal direction control module is further used for: in response to receiving the second target instruction, controlling the reagent box moving module to move according to the moving direction and/or the moving distance indicated by the second target instruction;
when the target detection module includes horizontal direction control module and waste liquid box motor, horizontal direction control module still is used for: and controlling the waste liquid box motor to rotate according to the moving direction and/or the moving distance indicated by the second target instruction in response to the received second target instruction so as to drive the waste liquid box to move.
9. The sequencer control system of claim 4, wherein when the target detection module comprises an LD module, the LD module is configured to control an LD light source in the LD module to emit laser light in response to receiving the second target instruction.
10. The sequencer control system of claim 4, wherein the sequencing subsystem further comprises a sequencer camera, the sequencer camera coupled to the vertical control module;
when the target detection module comprises a vertical direction control module, the vertical direction control module is configured to, in response to receiving the second target instruction, perform at least one of:
controlling a motor of the sequencer camera to move so as to drive a lens of the sequencer camera to move to a target position corresponding to the second target instruction indication target position information; wherein the target location comprises: the target location includes: a position where the accuracy of the sequencer camera matches a preset accuracy, and/or a position where the sequencer camera focuses on;
and controlling the power supply of the vertical direction control module to be disconnected.
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