CN115887543B

CN115887543B - Application of Rosa roxburghii polyphenol in preparing medicine for preventing or treating intestinal diseases

Info

Publication number: CN115887543B
Application number: CN202310043795.3A
Authority: CN
Inventors: 廖小军; 赵靓; 杨焕治; 刘澍雨; 王永涛; 饶雷
Original assignee: China Agricultural University
Current assignee: China Agricultural University
Priority date: 2023-01-29
Filing date: 2023-01-29
Publication date: 2023-06-02
Anticipated expiration: 2043-01-29
Also published as: CN115887543A

Abstract

The invention provides application of Rosa roxburghii polyphenol in preparing medicines for preventing or treating intestinal diseases, and has important significance for popularization and application of Rosa roxburghii polyphenol.

Description

Application of Rosa roxburghii polyphenol in preparing medicine for preventing or treating intestinal diseases

Technical Field

The invention relates to the field of medicine. In particular, the invention relates to application of Rosa roxburghii polyphenol in preparing medicines for preventing or treating intestinal diseases.

Background

Fructus Rosae NormalisRosa roxburghii Tratt) Is the fruit of the filature flowers of the perennial fallen leaves shrubs of the rosaceae, is the special fruit in southwest areas of China, is mainly rich in various antioxidant active ingredients such as Vitamin C (Vitamin C, vc), superoxide dismutase (Superoxide dismutase, SOD), polyphenol, polysaccharide and the like, is known as 'Sanwang fruit', and has extremely high medicinal value.

Intestinal diseases are a general term for various inflammatory diseases of intestinal tract, and are clinically manifested by repeated abdominal pain, abdominal distention, hematemesis, hematochezia, diarrhea, constipation, appetite decrease, malnutrition and various systemic complications, and death may occur when serious.

At present, correlation between Rosa roxburghii polyphenol and intestinal diseases is reported, and the research is yet to be carried out.

Disclosure of Invention

The present invention aims to solve at least one of the technical problems existing in the prior art to at least some extent. Therefore, the invention provides the application of the Rosa roxburghii polyphenol extract in preparing medicines for preventing or treating intestinal diseases, and has important significance for popularization and application of the Rosa roxburghii polyphenol.

The invention provides application of a Rosa roxburghii polyphenol extract in preparing medicines. According to an embodiment of the invention, the medicament is for preventing or treating intestinal diseases. The inventor of the present invention has found that the Rosa roxburghii polyphenol can improve the intestinal microenvironment and intestinal barrier function, and is helpful for preventing or treating intestinal diseases.

According to an embodiment of the invention, the intestinal disease is selected from inflammatory bowel diseases.

Inflammatory bowel Disease (Inflammatory bowel Disease, IBD) is a idiopathic intestinal inflammatory Disease that clinically manifests diarrhea, abdominal pain, bloody stool and is developed in the ileum, rectum, colon, including ulcerative colitis (Ulcerative Colitis, UC), crohn's Disease (CD), and the like.

According to an embodiment of the invention, the inflammatory bowel disease is selected from ulcerative colitis. The inventor finds that the Rosa roxburghii polyphenol extract has better preventing or treating effect on ulcerative colitis.

According to an embodiment of the invention, the medicament is for at least one of: relieving diarrhea or hematochezia, improving colon length shortening and weight reduction, improving immune response and attenuation function, inhibiting spleen swelling, protecting colon tissue integrity, promoting intestinal epithelial cell proliferation, promoting intestinal mucus increase, promoting production of antiinflammatory factors in colon tissue, inhibiting production of pro-inflammatory factors in colon tissue, increasing antioxidant enzyme content in colon tissue, improving intestinal barrier and promoting production of intestinal barrier proteins of colon epithelial cells.

According to an embodiment of the invention, the pro-inflammatory factor is selected from TNF- α, IL-6 and/or IL-1β; the anti-inflammatory factor is selected from IL-4 and/or IL-10; the antioxidant enzyme is selected from glutathione, superoxide dismutase, catalase and/or glutathione peroxidase.

According to an embodiment of the present invention, the method for obtaining the Rosa roxburghii polyphenol extract comprises: loading the roxburgh rose juice into macroporous resin for adsorption, eluting the macroporous resin with an eluent, and collecting effluent; performing rotary evaporation and freeze drying treatment on the effluent liquid to obtain freeze-dried powder; and re-dissolving the freeze-dried powder, performing solid phase extraction treatment on the obtained re-solution, collecting eluent, and drying to obtain the Rosa roxburghii polyphenol extract.

The inventor finds that the macroporous adsorption resin has better adsorption effect on the Rosa roxburghii polyphenol through a large number of experiments, and can effectively separate the Rosa roxburghii polyphenol by adsorbing the Rosa roxburghii juice with the macroporous adsorption resin, and remove substances such as polysaccharide. Meanwhile, the product has astringency due to the existence of polyphenol substances, and the astringency removal effect can be effectively achieved through the adsorption of macroporous adsorption resin. The eluent can be removed by rotary evaporation and freeze drying treatment of the effluent liquid, and the obtained freeze-dried powder has higher concentration of the Rosa roxburghii polyphenol after re-dissolution, thereby being beneficial to the subsequent solid phase extraction treatment and improving the yield and purity of the Rosa roxburghii polyphenol. The dried Rosa roxburghii polyphenol extract can be obtained through drying treatment, and is convenient to store, transport and use.

According to an embodiment of the invention, the macroporous resin is selected from AB-8 macroporous resins. Therefore, the Rosa roxburghii polyphenol can be specifically adsorbed, so that components such as polysaccharide and the like can be removed better, the Rosa roxburghii polyphenol is retained to the greatest extent, and meanwhile, the obtained Rosa roxburghii polyphenol extract has higher content of active components for preventing or treating intestinal diseases, and is beneficial to better exerting efficacy.

According to an embodiment of the present invention, the eluent is selected from 50-70% by volume of ethanol solution. Thereby, the active ingredients for preventing or treating intestinal diseases can be effectively eluted from the AB-8 macroporous resin.

According to an embodiment of the present invention, the solid phase extraction treatment comprises: loading the complex solution into a solid phase extraction column which is activated in advance, leaching the solid phase extraction column by using acid liquor, eluting the solid phase extraction column by using methanol, and collecting eluent. Therefore, the Rosa roxburghii polyphenol can be further separated and extracted, and the product yield and purity are improved.

According to an embodiment of the invention, the solid phase extraction column is selected from C ₁₈ A solid phase extraction column. Therefore, the Rosa roxburghii polyphenol can be further separated and extracted, and the product yield and purity are improved.

According to an embodiment of the present invention, the method for obtaining the Rosa roxburghii polyphenol extract comprises: the method comprises the steps of (1) using a constant force source roxburgh rose concentrated juice as a raw material, adsorbing the roxburgh rose concentrated juice by using macroporous resin AB-8, and collecting effluent liquid; eluting the effluent with 60 volume percent ethanol solution, and collecting eluent; subjecting the eluate to rotary evaporationFreeze-drying to obtain freeze-dried powder; re-dissolving the freeze-dried powder to obtain a re-solution; activation of C with 100% ethanol by volume and an acid solution with pH 2 ₁₈ A solid phase extraction column, wherein the complex solution is loaded into the solid phase extraction column, the solid phase extraction column is leached by acid liquor with the pH value of 2, the solid phase extraction column is eluted by 100 volume percent methanol, and eluent is collected; nitrogen blowing the eluent, collecting concentrated solution, and freeze drying to obtain the Rosa roxburghii polyphenol extract. The inventor obtains the method through a large number of experiments, the method can be used for efficiently extracting the Rosa roxburghii polyphenol, the yield is high, and the obtained Rosa roxburghii polyphenol extract has higher content of active ingredients for preventing or treating intestinal diseases, thereby being beneficial to better playing the efficacy.

According to an embodiment of the invention, the dosage form of the medicament is selected from a tablet, a liquid, a powder granule, a chewable tablet or a soft gel.

The term "treatment" is used to refer to obtaining a desired pharmacological and/or physiological effect, such as ameliorating a disease or disorder. The effect may be prophylactic in terms of completely or partially preventing the disease or symptoms thereof, and/or may be therapeutic in terms of partially or completely curing the disease and/or adverse effects caused by the disease. As used herein, "treatment" encompasses treatment of a disease in a mammal, particularly a human, including: (a) Preventing the occurrence of a disease or disorder in an individual susceptible to the disease but not yet diagnosed with the disease; (b) inhibiting disease, e.g., arresting disease progression; or (c) alleviating a disease, e.g., alleviating symptoms associated with a disease. As used herein, "treating" encompasses any administration of a drug to a subject to treat, cure, alleviate, ameliorate, alleviate or inhibit a disease in the subject, including, but not limited to, administration of a drug comprising a extract of a Rosa roxburghii polyphenol as described herein to a subject in need thereof.

The term "administering" as used herein refers to introducing a predetermined amount of a substance into a patient by some suitable means. The drug of the present invention may be administered by any common route as long as it can reach the intended tissue. Various modes of administration are contemplated, including peritoneal, intravenous, intramuscular, subcutaneous, cortical, oral, topical, nasal, pulmonary and rectal, but the invention is not limited to these exemplified modes of administration. However, since the peptide is digested and peptide bonds are broken upon oral administration, the active ingredient of the orally administered drug should be coated or formulated to prevent it from being degraded or destroyed in the stomach. Preferably, the medicament of the present invention may be administered as an injectable formulation. In addition, the medicaments of the present invention may be administered using a specific device that delivers the active ingredient to the target cells.

The frequency and dosage of administration of the medicament of the present invention may be determined by a number of relevant factors including the type of disease to be treated, the route of administration, the age, sex, weight and severity of the disease of the patient, and the type of medicament as an active ingredient. According to some embodiments of the invention, the daily dose may be divided into 1 dose, 2 doses or more in a suitable form to be administered 1, 2 or more times over the whole period of time, as long as a therapeutically effective amount is achieved.

The term "therapeutically effective amount" refers to an amount of a compound sufficient to significantly ameliorate some of the symptoms associated with a disease or disorder, i.e., an amount that provides a therapeutic effect for a given disorder and dosing regimen. For example, in cancer treatment, a drug or compound that reduces, prevents, delays, inhibits or retards any symptom of a disease or disorder should be therapeutically effective. A therapeutically effective amount of the drug is not required to cure the disease or condition, but will provide treatment for the disease or condition such that the onset of the disease or condition is delayed, prevented or prevented, or symptoms of the disease or condition are alleviated, or the period of the disease or condition is altered, or for example the disease or condition becomes less severe, or recovery is accelerated in an individual.

According to the embodiment of the invention, the effective dose of the Rosa roxburghii polyphenol extract in the medicament is 50-100 mg/kg of mouse weight/day, or 330-660 mg/60kg person weight/day.

According to an embodiment of the invention, the medicament further comprises pharmaceutically acceptable excipients, such as carriers, solvents, suspending agents or excipients. Exemplary excipients may be liquid or solid, including but not limited to: pH adjusting agents, surfactants, carbohydrates, adjuvants, antioxidants, chelating agents, ionic strength enhancers, preservatives, carriers, glidants, sweeteners, dyes/colorants, odorants, wetting agents, dispersants, suspending agents, stabilizers, isotonic agents, solvents, emulsifiers, sprays, compressed air or other suitable gases, or other suitable inactive ingredients for use with the pharmaceutically effective compounds. Examples of excipients include various lactose, mannitol, oils such as corn oil, buffers such as PBS, saline, polyethylene glycol, glycerol, polypropylene glycol, dimethylsulfoxide, amides such as dimethylacetamide, proteins such as albumin, mono-and oligosaccharides such as glucose, lactose, cyclodextrin and starch.

Advantageous effects

(1) The Rosa roxburghii polyphenol extract provided by the invention can not only effectively inhibit the DSS-induced colonitis symptoms such as weight loss, diarrhea, hematochezia and the like of mice; it also improves intestinal barrier function by promoting intestinal epithelial cell proliferation and intestinal mucus increase.

(2) The Rosa roxburghii polyphenol extract provided by the invention can open up a new diet or medicine intervention way for improving and treating intestinal diseases including inflammatory bowel disease, diarrhea, hematochezia and the like, improving intestinal barrier function and intestinal microenvironment, and has remarkable propulsion effect on the application of Rosa roxburghii polyphenol in the market.

Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.

Drawings

The foregoing and/or additional aspects and advantages of the invention will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:

FIG. 1 is a graph showing the experimental design of PBS group, DSS group and CL_PP group, weight change of mice, disease activity index, physical image of mice, spleen weight, and colon length of comparative example 2;

FIG. 2 is a graph showing comparison of colon tissue HE staining pathological states of mice in PBS group, DSS group and CL_PP group in example 3;

FIG. 3 is a comparison of the PBS group, DSS group and CL_PP group of mice colon tissue AB stained Goblet Cells (GCS) in example 4;

FIG. 4 is a graph showing comparison of PAS staining mucus in colon tissue of mice in PBS group, DSS group and CL_PP group in example 5;

FIG. 5 is a graph showing comparison of antioxidant substance contents in colon tissues of mice in PBS group, DSS group and CL_PP group in example 6;

FIG. 6 is a graph showing comparison of inflammatory factor content in colon tissue of mice in PBS group, DSS group and CL_PP group in example 7.

Detailed Description

The scheme of the present invention will be explained below with reference to examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the present invention and should not be construed as limiting the scope of the invention. The examples are not to be construed as limiting the specific techniques or conditions described in the literature in this field or as per the specifications of the product. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

Example 1

In this example, a Rosa roxburghii polyphenol extract was obtained according to the following method:

1. the method comprises the steps of (1) using a constant force source roxburgh rose concentrated juice as a raw material, adsorbing the roxburgh rose concentrated juice by using macroporous resin AB-8, and collecting effluent liquid;

2. eluting the effluent liquid with 60 volume percent ethanol solution, and collecting the eluent;

3. performing rotary evaporation and freeze drying treatment on the eluent to obtain freeze-dried powder;

4. re-dissolving the freeze-dried powder to obtain a re-solution;

5. activation of C with 100% ethanol by volume and acidified water with pH 2 ₁₈ Loading the complex solution into a solid phase extraction column, eluting the solid phase extraction column by using acidified water with the pH value of 2, eluting the solid phase extraction column by using 100 volume percent methanol, and collecting eluent;

6. nitrogen is used for flushing and removing liquid, concentrated liquid is collected and subjected to freeze drying treatment, and the Rosa roxburghii polyphenol extract is obtained, wherein the content of the Rosa roxburghii polyphenol reaches 91.62%.

Example 2 Rosa roxburghii polyphenol can improve inflammatory bowel disease in mice

Sodium dextran sulfate (DSS) induced ulcerative colitis in mice was used as a study model, which was set up as follows: healthy male C57BL/6J mice of 6 weeks of age were selected and kept in a standard 12 hour circadian cycle, constant temperature at 22℃and Specific Pathogen Free (SPF) environment. After 1 week of adaptive feeding, mice were randomly divided into normal group (PBS, 8), model group (DSS, 8) and rosa roxburghii polyphenol group (cl_pp+dss, 8). PBS group and DSS were gavaged with 0.01 mol/L PBS (0.1 ml/10g (mouse body weight)/day) after 1 week of adaptive feeding, and CL_PP group was gavaged with the Rosa roxburghii polyphenol extract obtained in example 1 (50 mg/kg (mouse body weight)/day). After 3 weeks of continuous gavage, 2.5% dss was added to the drinking water of 3 groups of mice, which were free to drink water for 7 days continuously to induce colitis. In the induction process, recording the weight and fecal state of the mice daily and hematochezia; after the experiment is finished, the eyeballs are picked up to obtain blood, the blood is placed in an EDTA anticoagulant centrifuge tube, and after standing and centrifugation, the blood plasma is obtained, the mice are dissected, and the colon and the spleen are picked up. Measuring the length of colon, weighing spleen, photographing, collecting colon content, preserving 2 mm colon tissue sample with 4% formalin fixed liquid, collecting other colon tissue and freezing and storing tube, placing into liquid nitrogen for freezing and storing, and after dissection, preserving colon tissue and intestinal tract content and other samples in a refrigerator at-80 ℃.

The inflammatory bowel disease changes in PBS, DSS, CL _pp these 3 groups of differently treated mice are shown in fig. 1, where a of fig. 1 is the experimental design process. FIG. 1 b shows the trend of weight change in mice after IBD molding, and it can be seen that the DSS mice showed a significant weight loss after 3 days of DSS induction compared with PBS mice, and the average body weight of DSS mice after 7 days of induction was 82.21.+ -. 9.78% of the initial body weight, and the average body weight of CL_PP mice was 92.11.+ -. 6.23% of the initial body weight, with significant differencesp<0.01. Thus, it is shown that the Rosa roxburghii polyphenol can significantly relieve the weight loss of mice induced by DSS.

Disease activity index (Disease activity index, DAI) is an important index for evaluating the severity of a colitis model in combination with the parameters of weight loss, fecal sparsity, hematochezia, etc. of mice. The Disease Activity Index (DAI) versus data for group 3 mice is shown in fig. 1 c, with higher DAI scores indicating more severe diarrhea and hematochezia in the mice. Fig. 1 d is a comparison of photographs taken before dissection of mice, and can be seen by combining the two figures c and d: compared with the DSS mice, the CL_PP mice with the lavage effect of the Rosa roxburghii polyphenol are obviously reduced in DAI, namely the colonitis symptoms of the mice are obviously improved, which indicates that the Rosa roxburghii polyphenol can obviously improve the disease activity index of the mice induced by the DSS.

Spleen is the largest immune organ of the mouse, and spleen coefficient (the ratio of weight of the spleen to weight) can effectively reflect the immune response and attenuation functions of the mouse to foreign substances, and is also a commonly used index in toxicology experiments. The spleen coefficient pairs of 3 mice, such as shown in e of fig. 1, can be seen in combination with the spleen photographs and data statistics of the mice: compared with the DSS mice, the CL_PP mice DAI of the intragastric Rosa roxburghii polyphenol, namely the spleen coefficient of the mice, is obviously reduced, which shows that the Rosa roxburghii polyphenol can obviously improve the immune response and the attenuation function of the mice induced by the DSS.

The length of the colon is another important reference index for evaluating the severity of colonic inflammation. As can be seen from f of fig. 1, in combination with the colon length photograph of the mice and the colon data comparison chart: compared with the DSS mice, the CL_PP mice infused with the gastric Rosa roxburghii polyphenol have longer colon length, namely the Rosa roxburghii polyphenol can obviously improve inflammatory symptoms such as shorter colon length, congestion, edema and the like caused by DSS induction.

As can be seen from fig. 1, the rosa roxburghii tratt polyphenol can effectively inhibit DSS-induced weight loss, diarrhea and hematochezia of mice, and relieve symptoms such as colon shortening, spleen swelling and the like.

EXAMPLE 3 Rosa roxburghii polyphenol can promote colon pathology in healthy mice

The effect of Rosa roxburghii polyphenol on the pathological state of colon tissue of mice was investigated using DSS-induced colitis of mice in example 2 as a study model. The colon pathology of mice was detected by Hematoxylin/Eosin (HE) staining. Specifically, as shown in fig. 2, a, b and c of fig. 2 are comparison graphs of intestinal epithelial tissues of 3 groups of mice, and d, e and f of fig. 2 are comparison graphs of colon mucosal layer thickness, colon crypt depth and colon histopathological scores of 3 groups of mice. As can be seen from fig. 2, cl_pp group mice, which were perfused with the rosa roxburghii polyphenol, showed a significant improvement in colon pathology compared to DSS group mice.

Example 4 Rosa roxburghii polyphenol can promote intestinal epithelial goblet cell proliferation in healthy mice

Goblet cells are a special class of intestinal epithelial cells that are capable of producing and secreting mucins into the intestinal lumen to form a mucous layer that maintains intestinal barrier function, preventing hyperimmune activation. The effect of Rosa roxburghii polyphenol on intestinal epithelial goblet cell proliferation of mice was investigated using DSS-induced colitis in example 2 as a study model. Morphological changes of mouse colon epithelial goblet cells were detected by Periodic acid-Schiff (PAS) staining. GCs quantitative analysis was performed on the result of PAS staining using Image J.

Specifically, as shown in fig. 3, a, b and c of fig. 3 are comparison graphs of intestinal epithelial goblet cells of 3 groups of mice, and d of fig. 3 is a comparison graph of data of area ratio of intestinal epithelial goblet cells of 3 groups of mice. As can be seen from fig. 3, cl_pp group mice, which were perfused with the rosa roxburghii polyphenol, showed a significant promotion of intestinal epithelial goblet cell proliferation as compared to DSS group mice.

Example 5 Rosa roxburghii polyphenol can promote intestinal mucus production in healthy mice

The mucus layer has the important function of preventing bacteria and other pathogenic antigens in intestinal tracts from entering the blood circulation of organisms and other tissues and organs. The intestinal mucus production of mice by Rosa roxburghii polyphenol was investigated using DSS-induced colitis in example 2 as a study model. The mice were analyzed for changes in colonic mucus production using an Alcian Blue (AB) staining method.

As shown in particular in fig. 4. Fig. 4 a, b and c are real object comparison diagrams of colon mucus barriers of 3 groups of mice, and fig. 4 d is a comparison diagram of colon mucus layer area ratio data of 3 groups of mice. As can be seen from fig. 4, cl_pp group mice, which were perfused with the rosa roxburghii polyphenol, exhibited significantly increased mucus compared to DSS group mice.

Therefore, the Rosa roxburghii polyphenol has the effects of promoting the proliferation of intestinal epithelial cells and increasing intestinal mucus, has important effects on the anti-infection effect and the immunity effect of the intestinal tract of the organism, and further plays an important role in the occurrence and development of various intestinal diseases such as inflammatory bowel diseases, cystic fibrosis, intestinal tumors and the like.

Example 6 Rosa roxburghii polyphenol can enhance the antioxidant capacity of the colon of mice, alleviate oxidative damage

IBD models constructed using DSS lead to the generation of large amounts of free radicals by diseased tissue of the colon, which in turn leads to oxidative damage to the colon. The effect of Rosa roxburghii polyphenol on alleviating colon oxidative damage in mice was investigated using DSS-induced colitis in example 2 as a study model. Representative oxidoreductases in plasma, such as total antioxidant capacity (T-AOC), propylene glycol (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and Glutathione Peroxidase (GPX), were tested using commercial kits (Solarbio, beijing, china).

As shown in particular in fig. 5. Fig. 5 a-f are graphs for comparing the detection of the contents of T-AOC, MDA, GSH, SOD, CAT and GPX in colon tissues of 3 groups of mice, and as can be seen from fig. 5, the content of antioxidant enzymes (glutathione, superoxide dismutase, catalase and glutathione peroxidase) in the colon of the CL_PP group of the lavage Rosa roxburghii polyphenol is improved and the content of peroxidation products (propylene glycol) is reduced compared with the content of the antioxidant enzymes (glutathione, superoxide dismutase, catalase and glutathione peroxidase) in the colon of the CL_PP group of the mice of the DSS group. Therefore, the Rosa roxburghii polyphenol can strengthen the oxidation resistance of the colon of the mouse and relieve the oxidative damage.

Example 7 Rosa roxburghii polyphenol can strengthen the anti-inflammatory ability of the colon of mice, and alleviate inflammatory injury

Inflammatory response is an important indicator of IBD, and the effect of rosa roxburghii polyphenol on alleviating inflammatory lesions in the colon of mice was explored using DSS-induced mice in example 2 as a model. The concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta), interleukin-4 (IL-4) and interleukin-10 (IL-10) in colon tissue were detected by ELISA (Shanghai ELISA Biotechnology Co., ltd.).

As shown in particular in fig. 6. FIG. 6 a-e are the concentrations of TNF- α, IL-6, IL-1β, IL-4 and IL-10, respectively, in colon tissue of 3 groups of mice. As can be seen from fig. 6, compared with the DSS group mice, the concentration of the promoting inflammatory factors TNF- α, IL-6 and IL-1β in colon tissue of the gavage cl_pp group mice was significantly reduced, and the concentrations of the inhibiting inflammatory factors IL-4 and IL-10 were significantly increased. The Rosa roxburghii polyphenol can strengthen the colon anti-inflammatory capability of mice and alleviate inflammatory injury.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.

Claims

1. Use of a rosa roxburghii polyphenol extract in the manufacture of a medicament, characterized in that the medicament is for preventing or treating ulcerative colitis;

the method for obtaining the Rosa roxburghii polyphenol extract comprises the following steps:

the method comprises the steps of (1) using a constant force source roxburgh rose concentrated juice as a raw material, adsorbing the roxburgh rose concentrated juice by using macroporous resin AB-8, and collecting effluent liquid;

eluting the effluent with 60 volume percent ethanol solution, and collecting eluent;

performing rotary evaporation and freeze drying treatment on the eluent to obtain freeze-dried powder;

re-dissolving the freeze-dried powder to obtain a re-solution;

activation of C with 100% ethanol by volume and an acid solution with pH 2 ₁₈ A solid phase extraction column, wherein the complex solution is loaded into the solid phase extraction column, the solid phase extraction column is leached by acid liquor with the pH value of 2, the solid phase extraction column is eluted by 100 volume percent methanol, and eluent is collected;

nitrogen blowing the eluent, collecting concentrated solution, and freeze drying to obtain the Rosa roxburghii polyphenol extract.

2. The use according to claim 1, wherein the medicament is for at least one of:

relieving diarrhea or hematochezia, improving colon length shortening and weight reduction, improving immune response and attenuation function, inhibiting spleen swelling, promoting intestinal epithelial goblet cell proliferation, promoting intestinal epithelial cell proliferation, promoting intestinal mucus increase, promoting the generation of antiinflammatory factors in colon tissue, inhibiting the generation of pro-inflammatory factors in colon tissue, increasing antioxidant enzyme content in colon tissue, and improving intestinal barrier;

the pro-inflammatory factor is selected from TNF-alpha, IL-6 and/or IL-1 beta;

the anti-inflammatory factor is selected from IL-4 and/or IL-10;

the antioxidant enzyme is selected from glutathione, superoxide dismutase, catalase and/or glutathione peroxidase.

3. The use according to claim 1, wherein the dosage form of the medicament is selected from the group consisting of tablets, liquids, powder granules or soft gels;

the medicine also contains pharmaceutically acceptable auxiliary materials;

the effective dose of the Rosa roxburghii polyphenol extract in the medicament is 50-100 mg/kg of mouse weight/day or 330-660 mg/60kg of human weight/day.

4. Use according to claim 3, characterized in that the tablet is selected from chewing tablets.