CN115885996B - Application of hinokitiol in preventing and controlling agricultural plant diseases - Google Patents
Application of hinokitiol in preventing and controlling agricultural plant diseases Download PDFInfo
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- CN115885996B CN115885996B CN202211405521.6A CN202211405521A CN115885996B CN 115885996 B CN115885996 B CN 115885996B CN 202211405521 A CN202211405521 A CN 202211405521A CN 115885996 B CN115885996 B CN 115885996B
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- leaf blight
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- 240000008574 Capsicum frutescens Species 0.000 abstract description 2
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- 244000061176 Nicotiana tabacum Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 15
- 239000002609 medium Substances 0.000 description 9
- 239000007788 liquid Substances 0.000 description 7
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- 244000000005 bacterial plant pathogen Species 0.000 description 6
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- AMNAZJFEONUVTD-KEWDHRJRSA-N (2s,3s,4s,5r,6r)-6-(4-amino-2-oxopyrimidin-1-yl)-4,5-dihydroxy-3-[[(2s)-3-hydroxy-2-[[2-(methylamino)acetyl]amino]propanoyl]amino]oxane-2-carboxamide Chemical compound O1[C@H](C(N)=O)[C@@H](NC(=O)[C@H](CO)NC(=O)CNC)[C@H](O)[C@@H](O)[C@@H]1N1C(=O)N=C(N)C=C1 AMNAZJFEONUVTD-KEWDHRJRSA-N 0.000 description 1
- 241000219068 Actinidia Species 0.000 description 1
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- 241000758794 Asarum Species 0.000 description 1
- 244000301850 Cupressus sempervirens Species 0.000 description 1
- FEACDOXQOYCHKU-UHFFFAOYSA-N Gougerotin Natural products CNCC(=O)NC1=NC(=O)N(C=C1)C2OC(C(O)C(NC(=O)C(N)CO)C2O)C(=O)N FEACDOXQOYCHKU-UHFFFAOYSA-N 0.000 description 1
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- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- GJJYQFPADNKBDY-UHFFFAOYSA-N Sabinol Natural products C=C1CCC2(C(C)C)C1(O)C2 GJJYQFPADNKBDY-UHFFFAOYSA-N 0.000 description 1
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- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
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- MDFQXBNVOAKNAY-HWOCKDDLSA-N sabinol Chemical compound C([C@@H](O)C1=C)C2(C(C)C)C1C2 MDFQXBNVOAKNAY-HWOCKDDLSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pharmaceutical chemistry, and discloses a natural product hinokitiol which can effectively prevent and treat agricultural plant diseases, wherein the natural product hinokitiol has certain treatment effects on rice bacterial leaf blight, cucumber bacterial leaf blight, konjak bacterial leaf blight, tobacco bacterial wilt, citrus canker, grape canker, tomato canker, kiwi fruit canker, apple canker, rice fine strip germ, tobacco mosaic virus, cucumber mosaic virus, capsicum virus, rice virus, tomato virus, sweet potato virus, melon virus, maize dwarf mosaic virus and the like, and provides an important scientific basis for developing and creating new pesticides.
Description
Technical Field
The invention relates to the technical field of medicinal chemistry, in particular to application of hinokitiol in preventing and treating agricultural plant diseases.
Background
As the population continues to grow, food safety is again becoming a rigid requirement, and plant microbial disease is one of the most serious food health problems at present. Yield losses of agricultural products caused by plant microbial diseases are statistically as high as 20% to 40% each year. The plant pathogenic bacteria are one of the most destructive plant parasitic organisms in plant microbial diseases, and mainly show symptoms such as necrosis, wilt, rot and the like, for example, rice bacterial leaf blight bacteria, citrus canker bacteria, kiwi fruit canker bacteria and the like, and directly influence the yield and quality of agricultural products; and plant viruses such as tobacco mosaic virus and cucumber mosaic virus, which are widely harmful. At present, chemical pesticides remain the main adjuvant for controlling plant microbial diseases, but the gradual decrease of their effectiveness, in particular the emergence and growth of multi-drug resistant microbial strains, makes the situation more complex. Therefore, there is an urgent need to develop novel pesticides with unique modes of action that are efficient, low toxicity, low residue to control plant microbial diseases.
Natural products are an important source for the creation of new pesticides. The natural active compounds have the advantages of rich sources, low price, less adverse reaction and the like, and the natural products become research hotspots for developing novel pesticides. Investigation has shown that Hinokitiol (Hinokitiol), a natural active compound having various pharmacological properties, is a natural derivative of tolhenone, and is first found in heartwood of cypress. Hinokitiol has various pharmacological actions including antibacterial, antifungal, anti-inflammatory, antioxidant, neuroprotective, antidiabetic and hepatoprotective effects. Hinokitiol has been used as an effective antibacterial agent in various industrial fields including cosmetics, foods and some health products such as hair water, toothpaste, eyelid cleanser, hair restoration agent and body lotion, importantly it is harmless to human health, and has been approved by the japanese government as a food additive. However, the effects of hinokitiol on plant bacteria and plant viruses have not been studied.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides application of hinokitiol in preventing and controlling agricultural plant diseases.
In order to achieve the above purpose, the invention is realized by the following technical scheme:
the invention discloses application of hinokitiol in preventing and controlling agricultural plant diseases.
Preferably, the agricultural plant diseases include plant bacterial and plant viral diseases.
Preferably, the agricultural plant disease is one or any combination of rice bacterial leaf blight, cucumber bacterial leaf blight, konjak bacterial leaf blight, tobacco bacterial wilt, citrus canker, grape canker, tomato canker, kiwi canker, apple canker, rice leptosphaeria, tobacco mosaic virus, cucumber mosaic virus, pepper virus, rice virus, tomato virus, sweet potato virus, melon virus and maize dwarf mosaic virus.
Preferably, the hinokitiol is a natural product.
Preferably, the structural formula of the hinokitiol is as follows:
the invention has the following beneficial effects:
the invention discloses a natural product hinokitiol which can effectively prevent and treat agricultural plant diseases, wherein the natural product hinokitiol has a certain treatment effect on rice bacterial leaf blight, cucumber bacterial leaf blight, konjak bacterial leaf blight, tobacco bacterial wilt, citrus canker, grape canker, tomato canker, kiwifruit canker, apple canker, rice fine strip bacteria, tobacco mosaic virus, cucumber mosaic virus, capsicum virus, rice virus, tomato virus, sweet potato virus, melon virus, maize dwarf mosaic virus and the like, and provides an important scientific basis for the research and development and creation of new pesticides.
Detailed Description
The following description will clearly and fully describe the technical solutions of the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated.
Example 1
The natural product hinokitiol shows excellent antibacterial activity against plant bacteria, and the activities against rice bacterial blight bacteria (Xoo), citrus canker bacteria (Xac) and kiwi fruit canker bacteria (Psa) are taken as examples below, but the application of the natural product hinokitiol against other bacteria is not limited.
Wherein, the structural formula of hinokitiol is:
EC 50 (median effective concentration) is an important index for evaluating the sensitivity of plant pathogenic bacteria to a compound, and is also an important parameter for setting the concentration of the compound when researching the action mechanism of the target compound. In the concentration gradient experiment, proper 6 concentrations are set by adopting a double dilution method, and finally the inhibition rate of the medicament to plant pathogenic bacteria and the medicament concentration are converted into logarithmic values, and the virulence curve is obtained by SPSS software regression analysis, so that EC is calculated 50 。
Testing effective medium concentration EC of hinokitiol on plant pathogenic bacteria by turbidimetry 50 The test subjects were rice bacterial leaf blight bacteria (Xoo), citrus canker bacteria (Xac) and kiwi fruit canker bacteria (Psa). DMSO was dissolved in the medium as a blank.
The specific operation is as follows: 1) Placing the monoclonals of Xoo, xac and Psa (originally cultured in NA solid culture medium) into NB culture medium, and shake-culturing at 28deg.C in 180rpm constant-temperature shaker until logarithmic phase is reserved; 2) The agents/compounds are formulated at different concentrations (e.g.: 10. 5, 2.5, 1.25, 0.625 and 0.3125. Mu.g/mL) of the toxic NB broth is added to the test tube and the OD of the corresponding concentration of the toxic sterile NB broth is determined 595 Values (noted as sterile medium OD values); 3) Adding 40 μl of bacterial liquid cultured to logarithmic phase into all treated tubes, respectively, shake culturing at 28deg.C in a shaking table at 180rpm to CK group OD 595 After the value is 0.6 to 0.8, the bacterial liquid with each concentration is measured on a spectrophotometer to determine the OD 595 Values (noted as OD values of the bacteria-containing medium); 4) The inhibition (%) was calculated using the following formula.
Corrected OD = sterile medium OD-sterile medium OD;
inhibition ratio = [ (corrected control medium bacterial liquid OD value-corrected toxic medium OD value)/corrected control medium bacterial liquid OD value ] ×100%.
The activity results are shown in Table 1.
TABLE 1 EC of the natural product hinokitiol against phytopathogenic bacteria 50
As can be seen from table 1, the natural product hinokitiol exhibits excellent inhibitory activity against plant pathogenic bacteria such as bacterial leaf blight of rice, canker of citrus and canker of kiwi fruit in an in vitro test. Its EC against three pathogenic bacteria (Rhizoctonia solani, uvularia tangutica and Uyghur actinidia) 50 0.940, 1.343 and 2.274. Mu.g/mL, respectively.
Example 2
The natural product hinokitiol shows a certain antiviral activity in terms of plant virus resistance, and the activity against tobacco mosaic virus (Tobacco Mosaic Virus, TMV) and cucumber mosaic virus (CucumberMosaic Virus, CMV) is taken as an example, but the application of the natural product hinokitiol against other viruses is not limited.
1. Determination of anti-tobacco mosaic Virus Activity
1. Virus purification and concentration determination:
the virus purification and concentration determination are performed according to tobacco mosaic virus SOP specifications compiled by a measuring room of the university of south opening element. After 2 times of polyethylene glycol centrifugation treatment, the concentration of the virus crude extract is measured, and the virus crude extract is refrigerated at 4 ℃ for standby.
2. Preparing hinokitiol solution:
weighing, adding DMSO to dissolve the raw materials to obtain 5×10 4 mg/L mother liquor, and then diluting the mother liquor to the required concentration by using an aqueous solution containing 1% of Tween 80; ningnanmycin preparation was also diluted in tween.
3. Living body protecting action:
and selecting the leaf tobacco with consistent growth vigor in 5-6 leaf period, and using the writing brush to lightly apply the medicament in the left half She Tushi and the solvent in the right half She Tushi with corresponding dosage as a control. And (3) performing wet culture in an illumination incubator, controlling the temperature to be 23+/-1%, diluting TMV virus liquid to a proper concentration by using a phosphoric acid buffer solution after illumination is performed for 10000Lux and 20 hours, manually rubbing and inoculating the TMV virus liquid on the leaves scattered with silicon carbide by using a gang pen, inoculating viruses on the whole leaves, naturally airing, and washing the leaves with clear water. Then, the culture was performed in an illumination incubator at a controlled temperature of 23.+ -. 1 ℃ under illumination of 10000Lux. 3 plants, 3-4 leaves per plant, were set per drug treatment. Repeating the method for 3 times for each medicament, recording the number of the lesions after 2 days, and calculating the control effect.
4. In vivo therapeutic action:
and (3) selecting 5-6 leaf stage heart leaf cigarettes with consistent growth vigor, diluting TMV virus liquid to a proper concentration by using phosphoric acid buffer solution, scattering silicon carbide on whole leaves, inoculating virus by using whole leaves of a gang pen, naturally airing, and washing by using clear water. After the leaves were dried, the left half of the leaves were coated with a brush pen Shi Yaoji and the right half She Tushi of the corresponding dose of solvent was used as a control. Then, the culture was kept in an incubator under illumination at a temperature of 23.+ -. 1 ℃ for 10000Lux under illumination for 2 days, and the number of generated dried spots was observed and recorded. 3 plants were set per treatment with 3-4 leaves per plant, and 3 replicates per agent were performed as described above.
5. Test results
The test results of the Tobacco Mosaic Virus (TMV) resistance of the natural product hinokitiol are shown in Table 2.
Wherein, the inhibition ratio (%) = [ (control number of dried spots-treated number of dried spots)/control number of dried spots ] ×100%.
TABLE 2 Activity of the natural product hinokitiol against tobacco mosaic Virus
As can be seen from table 2, sabinol also showed a certain inhibitory activity against tobacco mosaic virus. Therefore, the natural skeleton has extremely high research value in the discovery of novel pesticides for resisting plant pathogenic bacteria and plant viruses.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (1)
1. The application of hinokitiol in preventing and controlling agricultural plant diseases is characterized in that: the agricultural plant disease is one or any combination of rice bacterial leaf blight, citrus canker, kiwi fruit canker and tobacco mosaic virus.
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| CN202211405521.6A CN115885996B (en) | 2022-11-10 | 2022-11-10 | Application of hinokitiol in preventing and controlling agricultural plant diseases |
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| CN115885996B true CN115885996B (en) | 2024-04-09 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018981A (en) * | 2009-09-22 | 2011-04-20 | 上海汇库生物科技有限公司 | Spray disinfectant-deodorant agent comprising hinokitiol |
| CN103168777A (en) * | 2011-12-26 | 2013-06-26 | 东北林业大学 | Waterborne hinokitiol wood preservative and preparation method thereof |
| CN112868653A (en) * | 2021-01-19 | 2021-06-01 | 兰州大学 | Application of bactericidal composition containing hinokitiol and plant essential oil and main components thereof in preventing and treating plant diseases and mold |
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2022
- 2022-11-10 CN CN202211405521.6A patent/CN115885996B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102018981A (en) * | 2009-09-22 | 2011-04-20 | 上海汇库生物科技有限公司 | Spray disinfectant-deodorant agent comprising hinokitiol |
| CN103168777A (en) * | 2011-12-26 | 2013-06-26 | 东北林业大学 | Waterborne hinokitiol wood preservative and preparation method thereof |
| CN112868653A (en) * | 2021-01-19 | 2021-06-01 | 兰州大学 | Application of bactericidal composition containing hinokitiol and plant essential oil and main components thereof in preventing and treating plant diseases and mold |
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