CN115873984A - Gene qPH and qIL related to plant height of tobacco, linked SSR marker and application thereof - Google Patents
Gene qPH and qIL related to plant height of tobacco, linked SSR marker and application thereof Download PDFInfo
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Abstract
The invention discloses genes qPH and qIL related to tobacco plant height, a linked SSR marker and application thereof, wherein the characteristics related to the tobacco plant height comprise natural height characteristics of tobacco plants and pitch characteristics of the tobacco plants, and the genes for controlling the two characteristics are qPH and qIL respectively. The codominant SSR markers closely linked with the tobacco plant height related trait genes qPH and qIL are numbered as TM37949 and TM29165, and the nucleotide sequences of PCR amplification products are respectively shown as SEQ ID No.1 and SEQ ID No.2, SEQ ID No.3 and SEQ ID No. 4. The application is the application of the codominant SSR marker closely linked with the tobacco plant height related trait genes qPH and qIL in detecting whether the plant height related trait genes qPH and qIL exist in the tobacco genome DNA. The codominant SSR marker has the characteristics of rapidness, stability, accuracy and low cost, so that the molecular marker can be used as an application of qPH and qIL gene molecular marker assisted selection in breeding of related characters of tobacco plant height.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a codominant SSR marker closely linked with tobacco plant height related trait genes qPH and qIL and application thereof.
Background
The tobacco plant height character comprises the natural height and pitch of the plant, and belongs to the tobacco agronomic character. The plant height trait is also a quantitative trait susceptible to the environment, like other agronomic traits of tobacco. Previous QTL analysis on tobacco agronomic traits (including natural height and pitch of tobacco plants) is mainly carried out by focusing on a local tobacco genetic map constructed by using non-specific markers (such as RAPD, ISSR, AFLP, SCAR and the like), so that further application of QTL positioning results in tobacco breeding is limited to a great extent. Tong et al used a tobacco Doubled Haploid (DH) population and an SSR marker genetic map constructed based on this population to perform quantitative trait locus (TONG Z J, JIAO F C, WU X F, et al.mapping of quantitative trait loci in tobacco-cured tobaccos (Nicotiana tabacum L.) [ J ]. ACTA AGRONOMICA SINICA,2012,38 (8): 1407-1415.) mapping of 6 agronomic traits related to tobacco yield. QTL analysis is carried out on 7 yield-related agronomic traits, namely plant height, pitch, leaf number, stem circumference, stem leaf angle, waist leaf length and waist leaf width, of a recombinant inbred line group constructed based on K326 and Y3, such as Tongzhuang and the like, aiming at the plant height trait, 1 QTLs with larger effect values (R2 & gt 20%) is detected in two consecutive years, namely qPH (R2 =23.18%,2016 21.37%, 2017) and qIL (25.75%, 20.09%), and plant height-related trait genes (qPH and qIL) are detected on the No. 17 linkage group. Genes qPH and qIL, which are detected two years in succession and are related to the plant height of tobacco, are located in the region of about 1.62cM (34.522-32.906) between SSR markers PT53015 and PT53756 (Tongzhing, jiao Fangchan, chen Xuejun, wu Xingfu, fandonhuang, shouzhen. 7 QTL mapping analysis of the traits related to tobacco yield. Northwest plant journal, 2018,37 (7): 1235-1243.). Although the effect values of the genes qPH and qIL related to the plant height of the tobacco are high, the genes cannot be used as ideal molecular markers for genetic improvement of the plant height of the tobacco due to the relatively large genetic distance (> 1 cM) between SSR markers linked to the two sides of the genes.
Disclosure of Invention
The first purpose of the invention is to provide a codominant SSR marker closely linked with tobacco plant height related trait genes qPH and qIL; the second purpose is to provide the application of the codominant SSR marker closely linked with the tobacco plant height related trait genes qPH and qIL.
The invention is realized by adopting the following technical scheme.
The molecular markers TM37949 and TM29165 for detecting tobacco plant height related trait genes qPH and qIL are provided by the invention, the molecular markers adopt quantitative trait linkage analysis (QTL) method, co-dominant SSR markers linked with the tobacco plant height related trait genes qPH and qIL are obtained by screening in the whole genome range of tobacco, and the molecular marker can be used for auxiliary selection of the plant height related trait genes qPH and qIL to improve the molecular marker auxiliary selection efficiency and the high tobacco plant variety breeding efficiency.
The plant height related traits of the invention comprise natural height traits of tobacco plants and pitch traits of the tobacco plants, and the genes for controlling the plant height related traits of the tobacco plants are qPH and qIL and belong to quantitative trait gene loci.
The codominant SSR markers are closely linked with tobacco plant height related trait genes qPH and qIL, and the codominant SSR markers which are closely linked with the tobacco plant height related trait genes qPH and qIL are numbered as TM37949 and TM29165; the nucleotide sequences of PCR amplification products are respectively shown as SEQ ID No.1 and SEQ ID No.2, and SEQ ID No.3 and SEQ ID No. 4.
The primer sequences of 2 sites corresponding to the molecular marker are respectively as follows:
the TM37949 sequence is:
TM37949F：5’-CAAACAGAGGCGAACTCAGA-3’(SEQ ID NO.5)，
TM37949R：5’-CAGAACATCCCAGGGAAAAA-3’(SEQ ID NO.6)；
TM29165 sequence is:
TM29165F：5’-TTGCCTCGTAGCGATAGAAAA-3’(SEQ ID NO.7)，TM29165R：5’-GTCAAAACAACTGGAAATGCAA-3’(SEQ ID NO.8)。
the invention discloses application of the codominant SSR marker tightly linked with the tobacco plant height related trait genes qPH and qIL, and application of the codominant SSR marker tightly linked with the tobacco plant height related trait genes qPH and qIL in detecting whether the plant height related trait genes qPH and qIL exist in tobacco genome DNA.
The application steps of the invention comprise: respectively amplifying the tobacco genome DNA to be detected by using a primer with a TM37949 sequence and a primer with a TM29165 sequence,
the TM37949 sequence is:
TM37949F：5’-CAAACAGAGGCGAACTCAGA-3’(SEQ ID NO.5)，
TM37949R：5’-CAGAACATCCCAGGGAAAAA-3’(SEQ ID NO.6)；
TM29165 sequence is:
TM29165F：5’-TTGCCTCGTAGCGATAGAAAA-3’(SEQ ID NO.7)，TM29165R：5’-GTCAAAACAACTGGAAATGCAA-3’(SEQ ID NO.8)。
detecting the PCR amplification product; analyzing the PCR amplification product.
The PCR amplification product of the invention is: if the PCR amplification product simultaneously contains the sequences shown as SEQ ID No.1 and SEQ ID No.3, the tobacco plant to be detected contains dominant homozygous alleles qPHqPH and qILqIL with plant height related characters, and the genotype is marked as HH;
the PCR amplification product of the invention is: if the PCR amplification product simultaneously contains the sequences shown as SEQ ID No.2 and SEQ ID No.4, the tobacco plant to be detected contains recessive homozygous alleles qphqph and qilqil with plant height related characters, and the genotype is recorded as hh;
the PCR amplification product of the invention is: if the PCR amplification product contains the sequences shown as SEQ ID No.1 and SEQ ID No.2, or contains the sequences shown as SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.1, SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.3, SEQ ID No.1 and SEQ ID No.2, the genotype is marked as Hh, namely the heterozygous allele qPHqph and qILqqIll, qPHqPH and qILqIll, qPHqph and qILqIL containing the plant height related traits.
Compared with the prior art, the invention has the beneficial effects that: compared with the gene markers for the related characters of the tobacco plant height reported in the literature, the molecular markers provided by the invention have the remarkable advantages that: (1) The plant height character concepts are different, and reported documents define the plant height character of tobacco as the natural height of the tobacco plant; the plant height trait concept involved in the present invention includes both the natural height of tobacco plants and the pitch closely related to the natural height. (2) Compared with the QTL positioning result of the related characters of the tobacco plant height reported in the literature, the QTL positioning result of the related characters of the tobacco plant height provided by the invention has the advantages that the genetic distance between the tightly linked markers at two sides of the genes qPH and qIL related to the tobacco plant height is smaller, the positioning precision is higher, and the repeatability and the accuracy of the result are higher, so that the result of the auxiliary selection of the molecular marker used for the breeding of the tobacco plant height characters is more scientific and accurate.
Drawings
FIG. 1 shows a tobacco-based recombinant inbred line population (RILs _ F) 7:8 (ii) a QTL analysis curve chart of the plant height related characters of Honghuadajinyuan multiplied by Beiinhart 1000-1).
Wherein, QTL positioning software is as follows: winQTLCart v2.5; setting parameters: the positioning method comprises the following steps: composite Interval MappingQTL with iteration number of 1000 (persistence times = 1000), significance of 0.01 (Significice = 0.01) and step size of 0.5cM (Walk speed =0.5 cM). The abscissa in the figure is the genetic distance (in centimorgan cM); the ordinate is LOD value. The horizontal dashed line in the figure is the LOD value =7.2 at the 0.01 significance threshold; the highest point of the LOD curve is a major gene/QTL (qPH and qIL) of the related traits of the plant height of the tobacco.
Detailed Description
The present invention is further illustrated by the following examples and the accompanying drawings, but the present invention is not limited thereto in any way, and any modifications or alterations based on the teaching of the present invention are within the scope of the present invention. The examples do not indicate any particular technique or condition, and are performed according to the techniques or conditions described in the literature in the field or according to the relevant product specifications. The reagents or instruments used are conventional products which can be obtained by purchase, and are not indicated by manufacturers.
The invention takes a high-plant cigar variety Beinhart1000-1 and a short-plant flue-cured tobacco variety Hongda as parents, constructs a tobacco recombinant inbred line (RILs _ F) containing 341 strains through hybridization and continuous bagging inbreeding 7:8 ) In order to map the population, a Quantitative Trait Locus (QTL) method is utilized to screen and obtain a codominant SSR marker which is closely linked with tobacco plant height related trait genes qPH and qIL in the whole genome range of tobacco so as to accelerate the accurate and efficient utilization of molecular Marker Assisted Selection (MAS) in the breeding of new tobacco plant height trait strains.
The co-dominant SSR marker has the characteristics of accuracy, high efficiency, stability, convenience and low cost, so that the molecular marker can be used as an application of qPH and iIL gene molecular marker assisted selection in tobacco plant height trait breeding.
The codominant SSR markers tightly linked with the tobacco plant height related trait genes qPH and qIL are numbered TM37949 and TM29165, and the nucleotide sequences of PCR amplification products are respectively shown as SEQ ID No.1 and SEQ ID No.2, SEQ ID No.3 and SEQ ID No. 4.
The primer sequences of 2 sites corresponding to the molecular markers are respectively as follows:
the TM37949 sequence is:
TM37949F：5’-CAAACAGAGGCGAACTCAGA-3’，
TM37949R：5’-CAGAACATCCCAGGGAAAAA-3’；
TM29165 sequence is:
TM29165F：5’-TTGCCTCGTAGCGATAGAAAA-3’，
TM29165R：5’-GTCAAAACAACTGGAAATGCAA-3’。
the application of the co-dominant SSR markers tightly linked with the tobacco plant height related trait genes qPH and qIL is the application of the co-dominant SSR markers tightly linked with the tobacco plant height related trait genes qPH and qIL in detecting whether plant height related trait genes qPH and qIL exist in tobacco genomic DNA.
The application of the co-dominant SSR markers tightly linked with the tobacco plant height related trait genes qPH and qIL is to amplify the genomic DNA of the tobacco to be detected by using a primer of a TM37949 sequence and a primer of a TM29165 sequence respectively, detect a PCR amplification product, and if the PCR amplification product simultaneously contains sequences shown as SEQ ID No.1 and SEQ ID No.3, the co-dominant SSR markers are the dominant homozygous alleles qPHqPH and qILqIL of the tobacco plant containing the plant height related trait; if the PCR amplification product simultaneously contains the sequences shown as SEQ ID No.2 and SEQ ID No.4, the recessive homozygous allele qphq and qilqil with plant height related characters are contained in the tobacco plant to be detected; if the PCR amplification product contains the sequences shown as SEQ ID No.1 and SEQ ID No.2, or contains the sequences shown as SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.1, SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.3, SEQ ID No.1 and SEQ ID No.2, the heterozygous alleles qPHqph and qILqil, qPHqPH and qILqqil, qPHqph and qILqIL containing plant height related traits in the tobacco plant to be detected are the tobacco plant to be detected.
The invention is further illustrated by the following specific examples:
example 1
Screening codominant SSR markers linked with tobacco plant height related trait genes qPH and qIL in the whole genome range of tobacco by Quantitative Trait Locus (QTL) method
1. The experimental material is obtained by hybridizing and continuously selfing 341 recombinant selfing lines (RILs _ F) with excellent comprehensive properties and short plant flue-cured tobacco variety safflower Hongda as female parent and high plant cigar variety Beinhart1000-1 as male parent 7:8 ) As a genetically mapped population. 2. Parent and RILs _ F 7:8 Obtaining group plant height related trait phenotype data
The plant height related character survey is carried out according to tobacco industry standard YC/T369-2-10, wherein the plant height related character survey comprises the natural plant height and the plant pitch, and the specific measurement method comprises the following steps:
the height of the natural plant is as follows: and measuring the length from the stem base of the ground surface to the base of the first Chinese olive stem by using a rod ruler.
Pitch: the waist portion was measured at a position which occupies 1/3 of the height of the entire plant from bottom to top along the main stem of the tobacco plant, and the distance between the bases of two adjacent leaves was measured at the top and bottom of the waist portion of the tobacco plant, respectively, and then the average value was taken.
3. SSR marker analysis
Extracting tobacco genome DNA: the conventional CTAB method or plant tissue DNA extraction kit can be adopted, and the method can refer to the existing literature or the instruction in the kit.
PCR amplification and electrophoresis detection: the PCR amplification system is a conventional system and can refer to published documents, wherein the annealing temperature of the marker provided by the invention is 60 ℃; the information of PCR amplification program can be referred to relevant literature; the electrophoresis detection is also carried out by conventional methods, and reference is made to published relevant documents.
341 RILs _ F were tested against SSR markers developed in this laboratory 7:8 And (3) carrying out genotype analysis and linkage analysis on the sample, and drawing a high-quality tobacco SSR genetic linkage map which contains 24 linkage groups, is uniformly distributed with 1974 SSR markers, and covers the tobacco genome length of 3213.138 cM. Using the map and the genotype value of SSR marker as RILs _ F 7:8 Genotype values of the population were used for the next QTL linkage analysis.
4. Whole genome QTL analysis of tobacco plant height related trait genes (qPH and qIL)
RILs _ F pair by using QTL positioning analysis software WinQTLCart v2.5 7:8 Genotype data and phenotype data of the population, and whole genome QTL scanning is carried out on the tobacco plant height related trait genes qPH and qIL. Wherein, the related parameters are set as follows: the positioning method comprises the following steps of: composite Interval Mapping QTL with a number of iterations of 1000 (persistence times = 1000), significance of 0.01 (Significice = 0.01) and step size of 0.5cM (Walk speed =0.5 cM). Finally, localization at 111.40cM of linkage group No. 17 resulted in 3 years (2020, 2021 and 2022) consecutive smoke under genome-wide LOD =7.2 conditions1 major QTL (named qPH and qIL) of grass height related traits. This major QTL can account for the rate of phenotypic variation of about 26.52% (qIL, 2022) to 50.05% (qPH, 2022) with LOD values of about 27.11 (qIL, 2022) to 61.21 (qPH, 2022), as detailed in fig. 1 and table 1.
TABLE 1 statistics of information about QTL (qPH and qIL) related to plant height of tobacco
Example 2
Co-dominant linked markers in RILs _ F 8:9 Validation in population Individual plants
Firstly, the obtained codominant SSR markers TM37949 and TM29165 closely linked with the two sides of the tobacco plant height related trait genes qPH and qIL are used for aligning RILs _ F at the seedling stage 8:9 Genotype analysis is carried out on a single plant of a population (Honghuadajinyuan multiplied by Beiinhart 1000-1) to obtain RILs _ F 8:9 Genotype data for individual plants of the population. And secondly, measuring the plant height related traits of each strain according to the tobacco industry standard YC/T369-2-10. Finally, 341 RILs _ F were analyzed 8:9 The genotype data of the population and the phenotypic values of the plant height related traits are found that the genotype values of the two co-dominant SSR markers TM37949 and TM29165 disclosed by the invention are completely consistent with the phenotypic values, namely, the consistency rate reaches 100%. The specific analysis method comprises the following steps: when the natural plant height and the pitch phenotype value of each strain are obtained by measurement and are higher than or equal to the high-value parent Beiinhart 1000-1, the genotype of the strain also simultaneously presents sequences shown as SEQ ID NO.1 (208 bp) and SEQ ID NO.3 (169 bp), namely dominant homozygous alleles qPHqPH and qILqIL containing plant height related traits of the strain; when the natural plant height and the pitch phenotype value of each obtained strain are identified to be equal to or lower than the low-value parent safflower large gold, the genotype of the strain also simultaneously presents sequences shown as ID NO.2 (240 bp) and SEQ ID NO.4 (177 bp), namely the recessive homozygous allele qphqph and qilqil containing plant height related characters of the strain; and when the natural plant height and pitch phenotype value of each line obtained by identification are between the low-value parent safflower large golden dollar and the high-value parent Beinhart1000-1, i.e. with the next generation (F) 1 ) When the strains are similar, the genotypes of the strains simultaneously present sequences shown as SEQ ID NO.1 and SEQ ID NO.2, or contain sequences shown as SEQ ID NO.3 and SEQ ID NO.4, or contain sequences shown as SEQ ID NO.1, SEQ ID NO.3 and SEQ ID NO.4, or contain sequences shown as SEQ ID NO.3, SEQ ID NO.1 and SEQ ID NO.2, namely the hybrid alleles qPHqph and qqqqqqqil, QPHQHPl and qILqil, qPHqph and qILqIL containing plant height related traits of the strains.
The above results show that the co-dominant markers TM37949 and TM29165 are closely linked with the tobacco plant height related trait genes qPH and qIL respectively, the two markers are positioned at two sides of the target gene/QTL (qPH and qIL), and the genetic distance between the two markers is about 0.50cM. By utilizing the two co-dominant closely-linked SSR markers, the identification of the plant height related traits of the tobacco at the seedling stage can be accurately, efficiently, conveniently and low-cost realized, and the genotype state of the plant height related traits in the plant to be detected can also be clearly identified, so that the scientificity and the predictability of the breeding of new varieties of tobacco with the plant height related traits are improved, and the breeding process is accelerated.
SEQ ID NO.1：
CAAACAGAGGCGAACTCAGAATTTTTACGTGACGGGATATATTTAAATTAACCATTTTTA
AGGTATATATATATATATATATAAGATTTCAGCCGAACATATGGGTCCGCCTCTGTGTACAA
ACATGCTTTATTGATGTTGAACAACCTATAGCTAGCCTCTGCCCTCTCTAAAAGTTTTCTT
CGCTTTTTTTCCCTGGGATGTTCTG
SEQ ID NO.2：
CAAACAGAGGCGAACTCAGAATTTTTACGTGACGGGATATATTTAAATTAACCATTTTTA
AGGTATATATATATATATATATATAAGATTTCAGCCGAAATTTACGGGGTCCGTGACCCCTC
ACCCATACATATGGGTCCGCCTCTGTGTACAAACATGCTTTATTGATGTTGAACAACCTAT
AGCTAGCCTCTGCCCTCTCTAAAAGTTTTCTTCGCTTTTTTTCCCTGGGATGTTCTGSEQ ID NO.3：
TTGCCTCGTAGCGATAGAAAAGATATGGTCTCTAACGAAAATGACTGATTGCCCATTTTT
GTTGGTCTTTGACTGGTTGGACAAAGATCTGTTGATAGCTATATATATATATATATATATATG
CTCACATTCTGATATTCAAGAAAGTTGCATTTCCAGTTGTTTTGACSEQ ID NO.4：
TTGCCTCGTAGCGATAGAAAAGATATGGTCTCTAACGAAAATGACTGATTGCCCATTTTT
GTTGGTCTTTGACTGGTTGGACAAAGATCTGTTGATAGCTATATATATATATATATATATATA
TATATATGCTCACATTCTGATATTCAAGAAAGTTGCATTTCCAGTTGTTTTGACSEQ ID NO.5：
CAAACAGAGGCGAACTCAGA
SEQ ID NO.6：
CAGAACATCCCAGGGAAAAA
SEQ ID NO.7：
TTGCCTCGTAGCGATAGAAAA
SEQ ID NO.8：
GTCAAAACAACTGGAAATGCAA
The foregoing is only a part of the specific embodiments of the present invention, and the specific contents or common general knowledge in the schemes are not described herein too much (including but not limited to the shorthand, abbreviation, units commonly used in the art). It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and it is obvious for those skilled in the art that all the technical solutions obtained by using the equivalent substitution or the equivalent change fall within the protection scope of the present invention. The scope of the claims of the present application shall be defined by the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (8)
1. The tobacco plant height related trait genes qPH and qIL are characterized in that the plant height related trait comprises a natural height trait of a tobacco plant and a pitch trait of the tobacco plant, genes controlling the tobacco plant height related trait are qPH and qIL, and belong to quantitative trait gene loci.
2. A codominant SSR marker closely linked with tobacco plant height related trait genes qPH and qIL is characterized in that the codominant SSR marker closely linked with tobacco plant height related trait genes qPH and qIL is numbered as TM37949 and TM29165; the nucleotide sequences of PCR amplification products are respectively shown as SEQ ID No.1 and SEQ ID No.2, SEQ ID No.3 and SEQ ID No. 4.
3. The codominant SSR marker tightly linked with the tobacco plant height related trait genes qPH and qIL as claimed in claim 2, wherein the primer sequences of 2 sites corresponding to the molecular marker are respectively:
the TM37949 sequence is:
TM37949F：5’-CAAACAGAGGCGAACTCAGA-3’(SEQ ID NO.5)，
TM37949R：5’-CAGAACATCCCAGGGAAAAA-3’(SEQ ID NO.6)；
TM29165 sequence is:
TM29165F：5’-TTGCCTCGTAGCGATAGAAAA-3’(SEQ ID NO.7)，
TM29165R：5’-GTCAAAACAACTGGAAATGCAA-3’(SEQ ID NO.8)。
4. the use of a codominant SSR marker tightly linked to tobacco plant height-related trait genes qPH and qIL as claimed in claim 2 or 3, wherein said codominant SSR marker tightly linked to tobacco plant height-related trait genes qPH and qIL is used for detecting whether plant height-related trait genes qPH and qIL exist in tobacco genomic DNA.
5. The use of a co-dominant SSR marker tightly linked to tobacco plant height-related trait genes qPH and qIL as claimed in claim 4, wherein the steps of said use comprise: respectively amplifying the genomic DNA of the tobacco to be detected by using a primer of a TM37949 sequence and a primer of a TM29165 sequence,
the TM37949 sequence is:
TM37949F：5’-CAAACAGAGGCGAACTCAGA-3’；
TM37949R：5’-CAGAACATCCCAGGGAAAAA-3’；
TM29165 sequence is:
TM29165F：5’-TTGCCTCGTAGCGATAGAAAA-3’；
TM29165R：5’-GTCAAAACAACTGGAAATGCAA-3’；
detecting the PCR amplification product; analyzing the PCR amplification product.
6. The use of claim 5, wherein the PCR amplification product is: if the PCR amplification product simultaneously contains the sequences shown as SEQ ID No.1 and SEQ ID No.3, the tobacco plant to be detected contains dominant homozygous alleles qPHqPH and qILqIL with plant height related characters, and the genotype is marked as HH.
7. The use of claim 5, wherein the PCR amplification product is: if the PCR amplification product simultaneously contains the sequences shown as SEQ ID No.2 and SEQ ID No.4, the recessive homozygous alleles qphPh and qilqil with plant height related characters are contained in the tobacco plant to be detected, and the genotype is recorded as hh.
8. The use of claim 5, wherein the PCR amplification product is: if the PCR amplification product contains the sequences shown as SEQ ID No.1 and SEQ ID No.2, or contains the sequences shown as SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.1, SEQ ID No.3 and SEQ ID No.4, or contains the sequences shown as SEQ ID No.3, SEQ ID No.1 and SEQ ID No.2, the genotype is recorded as Hh, namely the heterozygous alleles qPHqph and qILqil, qPHqPH and qILqIL, qPHqph and qILqIL containing the plant height related traits.
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