CN115873682A - Prefabricating device and method for sample to be detected - Google Patents

Abstract

The invention relates to the technical field of automation, in particular to a prefabricating device and a prefabricating method for a sample to be detected, wherein the prefabricating device for the sample to be detected comprises a rack, and a tube moving device and a liquid moving device which are arranged on the rack: move a tub device and include the test tube feed bin, get the liquid platform and set up the mechanical hand of moving a tub above it, move the liquid device and include dropping liquid platform, consumptive material feed bin and set up the mechanical hand of moving a tub above that. The prefabricating device and the prefabricating method for the sample to be detected integrate a series of functions of tube moving, cover opening, liquid taking, liquid dropping, cover taking, cover pressing and the like of the cracked sample, fully-automatic mechanical operation can greatly reduce the requirements of related professionals, save medical resources and reduce epidemic prevention cost.

Description

Prefabricating device and method for sample to be detected

Technical Field

The invention relates to the technical field of automation, in particular to a prefabricating device and method for a sample to be detected.

Background

In recent years, new and emergent infectious diseases have the characteristics of multiple sources, fast transmission, wide spread and the like, and bring great challenges to infectious disease prevention and control and social stability. Since 2019, frequent outbreaks of new coronary pneumonia epidemic situations and global pandemics are more likely to promote monitoring and early warning of major infectious diseases to a new height. With the implementation of foreign 'natural' group immunization strategies, domestic prevention and control face unprecedented challenges. However, with the continuous variation and the enhancement of infectivity of new coronavirus, the difficulty of epidemic prevention and control is continuously increased, and especially for the places such as customs and primary medical institutions, the establishment of a healthy major infectious disease pathogen nucleic acid detection system becomes especially critical.

However, the existing nucleic acid detection system still has a large space, the nucleic acid detection mainly depends on professional institutions, large-scale, high-throughput and automatic equipment is mostly adopted, and the sample pretreatment steps are complicated, time-consuming and difficult to be suitable for on-site rapid diagnosis.

In recent years, a series of pathogenic nucleic acid detection platforms with automatic sample in-and-result out characteristics have appeared, but the detection platform is high in cost (enzymatic amplification is needed), limited in flux (mostly single target), or insufficient in key detection performance (such as sensitivity which cannot reach single copy/test), and cannot completely meet the requirements of on-site rapid real-time diagnosis.

The novel coronavirus (2019-nCoV) is exploded in a large scale worldwide, so that the nucleic acid detection enters a public field of view. Nucleic acid detection is a well-established "gold standard" in the world of new coronary diagnostic methods. At present, the conventional nucleic acid detection comprises 4 processes of sampling and pretreatment, nucleic acid extraction, PCR amplification and data analysis, and depends on professional laboratories, precise PCR equipment and professional operators, but the problems of infection of medical personnel, virus/reagent cross contamination, a plurality of independent spaces of reagents in the detection process, unsatisfactory consistency of detection results and the like cannot be avoided, so that the research and development of an integrated machine for rapidly detecting nucleic acid are urgent.

The existing integrated nucleic acid detection machine is mainly divided into the following three types: in-line automation systems, cassette-type all-in-one machines, and other types of all-in-one machines (amplification-free, nucleic acid-free, etc.). The production line automation system is the most mature integrated nucleic acid detector, the development is mainly based on the idea of replacing manpower with mechanical automation, the part of manual operation is reduced to only need to place reagent consumables and sample tubes, and the detection system consists of 4 modules of sample introduction, sample transfer, sample separation, purification, amplification and amplification detection. The automation of full flow of sample pipetting, sample preparation, amplification detection, result calculation and uploading can be realized, the protection force of operators is effectively improved, exogenous pollution is avoided, and the potential of POCT application is limited by the volume of the equipment.

A foreign representative product Roche cobas 8800 has a 24-hour detection flux as high as 2880; the 'green tillage No. one' of the Yangtze river of the sea above China is taken as a representative, only 4 times of manual consumable material transfer are needed after 24-hour continuous work, professional detection manpower can be greatly saved, and the single-tube detection flux reaches 4500 every day. The cassette type integrated machine integrates all functions of a production line system into one cassette, so that the volume of the equipment is greatly reduced, the detection flow is accelerated, and the portability is improved.

A foreign representative product cepheid definition 80-80 defines a molecular POCT product concept of 'sample in and result out', and is based on the composition of a special microfluidic reagent card box and a building block type accumulated reaction module, wherein the sample treatment, nucleic acid extraction and purification, system configuration and amplification detection are all completed in a special kit, the 24-hour detection flux can reach 768, and the follow-up detection can be realized;

domestic Automolec 3000 is the peak of the current domestic molecular diagnosis all-in-one machine, not only integrates nucleic acid extraction, purification, amplification and detection into one machine, but also realizes 'sample random detection'. The limitation that the traditional high-flux all-in-one machine can only detect according to batches is broken through.

Along with the continuous improvement of the detection speed and the portable requirement, amplification-free and hands-free nucleic acid integrated detection equipment is continuously emerged, and an integrated micro-fluidic card box free of nucleic acid extraction is developed by Qinghua university through optimizing a cracking process and a rear-end amplification reagent, so that 'sample input and result output' can be realized within 30 minutes; based on the characteristics of target specificity recognition and non-specific cleavage activation of a CRISPR-Cas13a system, a novel coronavirus automatic detection device within 9 minutes is developed in Japan.

In conclusion, related products at home and abroad are in a variety, and the products at home and abroad have little advantages in the aspects of mechanical precision control and new technology development and application, but most of the products are in the research and development level, and the market lacks related data and practices for large-scale application, so that a great improvement space is provided. Domestic products have made more thinking in PCR amplification modules and flux expansion and protection modules, and especially, more research needs to be invested in an automatic all-in-one machine which can be practically popularized and applied in a basic level.

Aiming at the trend of frequent outbreaks of new and sudden infectious diseases, the development trend of the integrated machine for quickly detecting nucleic acid tends to be changed from a large-volume production line type automation to a miniature automatic cassette type detection device, and finally, a new detection method and a new technology are fused to promote the development of the integrated, full-automatic and miniature molecular diagnosis equipment to be quick, accurate and high-flux.

Disclosure of Invention

In view of the above, the present invention provides a device and a method for preparing a specimen to be tested, which at least partially solve the problems in the prior art.

The utility model provides a ready-to-examine sample preparation device, includes frame and sets up move tub device and the liquid-transfering device on it:

the tube moving device comprises a test tube bin, a liquid taking platform and a tube moving manipulator arranged above the liquid taking platform,

the liquid-transfering device comprises a liquid-transfering platform, a consumable material bin and a liquid-transfering manipulator arranged on the liquid-transfering platform,

the tube moving manipulator is used for moving the nucleic acid extraction tube filled with the cracking sample in the test tube bin to the liquid taking table and opening the cover;

move liquid mechanical hand be used for with eight connecting tubes in the consumptive material feed bin are transferred to the dropping liquid platform, and follow it drips into to get liquid in the nucleic acid extraction tube eight connecting tubes obtain waiting to examine the sample.

Preferably, the liquid extraction platform is of a rack structure, and the lower part of the liquid extraction platform is provided with a clamping jaw six for clamping the nucleic acid extraction tube.

Preferably, the pipe moving manipulator includes:

an X-axis driver V for driving the rack to move along the left and right directions;

a fifth Y-axis driver for driving the rack to move back and forth;

a Z-axis driver V for driving the rack to move up and down;

a third rotating claw for clamping the nucleic acid extracting tube and rotating the cover.

Preferably, the consumable bin comprises at least one of the following consumables:

the eight-connecting pipe is used for containing a sample to be detected taken out from the nucleic acid extraction pipe;

the eight-connection cover is used for sealing the eight-connection pipe;

and the liquid-transferring sleeve is used for taking liquid from the nucleic acid extracting tube and dripping the liquid into the eight-connected tube.

Preferably, the pipette robot comprises:

the X-axis driver VI is used for driving the X-axis driver VI to move along the left and right directions of the rack;

a sixth Y-axis driver for driving the rack to move in the front-back direction;

a Z-axis driver VI for driving the rack to move up and down;

a clamping jaw seven for moving the eight-connection pipe to the liquid dropping table;

and the pipettor is used for operating the pipette sleeve to take liquid from the nucleic acid extraction tube on the liquid taking platform and dripping the liquid into the eight-connected tubes on the liquid dripping platform.

Preferably, the pipetting manipulator is further provided with an eight clamping jaw for transferring the eight-connecting cover to the eight-connecting pipe.

Preferably, the capping device further comprises:

the gland table is used for placing an eight-connection pipe with an eight-connection cap, and a linear driving mechanism for driving the gland table to move to the lower part of the gland machine is arranged at the lower part of the gland table;

and the capping machine is used for capping the eight-connection cap into the eight-connection pipe.

Preferably, a cannula recovery device is included, said cannula recovery device containing a disinfectant for storing used pipette cannulae.

Preferably, the device also comprises a killing device for killing the sample before and after detection so as to prevent the sample to be detected from being polluted or leaked.

Preferably, the consumable stock bin is of a drawer-shaped structure and can be pulled away from the rack in the left and right directions of the rack so as to replace the consumable.

Preferably, the fifth X-axis driver, the fifth Y-axis driver, the fifth Z-axis driver, the sixth X-axis driver, the sixth Y-axis driver, the fifth Z-axis driver and the sixth Z-axis driver are all linear driving mechanisms, and the driving modes are electric, pneumatic or hydraulic driving modes.

The utility model provides a carry out the prefabricated method of examining the sample of examining through above-mentioned prefabricated device of sample of examining, includes and moves a tub link, move liquid link and gland link, wherein:

the tube moving ring section mainly comprises the following actions that a nucleic acid extraction tube filled with a cracking sample in the test tube bin is moved to a liquid taking table through a tube moving manipulator and the cover of the liquid taking table is opened;

the pipetting link mainly comprises the following actions of transferring eight connected tubes in the consumable material bin to a liquid dripping table through a pipetting manipulator, and taking liquid from the nucleic acid extraction tube and dripping the liquid into the eight connected tubes;

the capping step mainly comprises the following actions of capping eight-connected caps into the eight-connected tubes through a capping machine.

Preferably, the pipe moving ring section comprises the following steps:

firstly, a tube moving manipulator moves a nucleic acid extraction tube filled with a cracking sample in a test tube bin to a liquid taking table;

step two, clamping the nucleic acid extracting tube by a clamping jaw six of the liquid taking table;

and thirdly, unscrewing an enrichment cover of the nucleic acid extraction tube by a rotating claw III of the tube moving manipulator.

Preferably, the pipe moving ring section further comprises the following steps:

step four, after liquid taking is finished, covering the tube cover of the nucleic acid extraction tube by the rotating claw III of the tube moving manipulator;

fifthly, clamping the nucleic acid extraction tube by a clamping jaw six of the liquid taking table, and driving the enrichment cover to rotate by a rotating jaw three so as to tighten the enrichment cover;

and sixthly, loosening the nucleic acid extraction tube by using the clamping jaw six of the liquid taking platform, and sending the nucleic acid extraction tube back to the test tube bin by using the tube moving manipulator.

Preferably, the pipetting step comprises the following steps:

moving eight connecting pipes in the consumable material bin to the liquid dropping platform by using a clamping jaw seven of the liquid transferring manipulator;

step two, a liquid transfer sleeve is arranged in the test tube bin by a liquid transfer machine of the liquid transfer manipulator;

and step three, operating the liquid-transferring sleeve to take liquid from the nucleic acid extracting tube on the liquid-taking platform and dripping the liquid into the eight-connected tubes on the liquid-dropping platform by the liquid-transferring manipulator of the pipe-transferring manipulator.

Preferably, the pipetting step further comprises the following steps:

and fourthly, throwing the used liquid-transferring sleeve into a sleeve recovery device by the liquid-transferring machine of the pipe-transferring manipulator.

Preferably, the capping ring comprises the following steps:

moving an eight-connection cover in the test tube bin to an eight-connection tube positioned on the liquid dropping platform by using a clamping jaw eight of the liquid transferring manipulator;

step two, the clamping jaw six of the liquid transfer manipulator transfers the eight-connected pipes covered with the eight-connected covers to a capping table from the liquid dropping table;

step three, the capping table conveys the eight-connected pipes covered with eight-connected caps to the lower part of the capping machine through a linear driving mechanism;

and step four, pressing down by the capping machine, and capping the eight-connection cap into the eight-connection pipe.

Preferably, the method further comprises a sterilization step, namely, before and after the sample to be detected is prefabricated, the device for the sample to be detected is sterilized through at least one of ultraviolet rays, high-temperature steam and a disinfectant.

Compared with the prior art, the prefabricating device and the prefabricating method for the sample to be detected have the following advantages that:

(1) Full-automatic mechanical operation, medical resources are saved, and requirements of professionals are reduced. The prefabricating device for the sample to be detected integrates a series of functions of tube moving, cover opening, liquid taking, liquid dropping, cover taking, cover pressing and the like of cracking the sample, is operated in a full-automatic and mechanical mode, can greatly reduce requirements of related professionals, saves medical resources, and reduces epidemic prevention cost.

(2) The operation of the closed space and the complete disinfection and killing measures avoid cross infection. The prefabricating device for the sample to be detected is completed in a relatively closed space in a rack, has a series of sterilization and disinfection measures such as high-temperature sterilization, ultraviolet lamp sterilization and the like which meet the national standard, can effectively prevent cross contamination, greatly improves epidemic situation prevention and control efficiency, and reduces the infection risk of people.

(3) The structure is compact, and multi-node, multi-level and rapid deployment can be realized. The prefabricating device for the sample to be detected has the advantages of compact structure, small floor area, light weight and flexible arrangement, can form a full-automatic unmanned nucleic acid sampling and detecting system, has high system modularization degree, is extremely easy to deploy aiming at the existing complex, multi-level, distributed and emergent epidemic situation prevention and control requirements of domestic epidemic situations, can be rapidly popularized to urban communities, schools, gardens, hotels, construction sites, traffic entrances, large business supermans, urban and rural junctions and vast rural areas, and can build a strong barrier for basic epidemic situation prevention and control.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a prefabricated device for a sample to be detected, which is provided by the embodiment of the invention and installed on a whole machine;

FIG. 2 is a schematic view of a sealing plate on the hidden rack of FIG. 1;

FIG. 3 is a first schematic view of the bottom structure of the hidden rack of FIG. 2;

FIG. 4 is a second schematic diagram of the bottom structure of the hidden rack shown in FIG. 2;

FIG. 5 is a first schematic view of a configuration of a device for preparing a sample to be tested in a nucleic acid detecting device according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a device for preparing a sample to be examined in the nucleic acid detecting device according to the embodiment of the present invention;

FIG. 7 is a first schematic structural diagram of a tube moving device in a device for preparing a sample to be examined according to an embodiment of the present invention;

FIG. 8 is a second schematic structural diagram of a tube moving device in the device for preparing a sample to be examined according to the embodiment of the present invention;

FIG. 9 is a first schematic view of a liquid-transferring device in the apparatus for preparing a specimen to be examined according to the embodiment of the present invention;

FIG. 10 is a second schematic structural diagram of a pipetting device in the apparatus for preparing samples to be examined according to the embodiment of the present invention.

Description of the reference numerals:

1-a frame;

11-a waste collection device;

12-a killing device;

13-identification verification means;

14-an isolation gate arrangement;

2-a sample pre-treatment device;

21-a sample enrichment device;

22-a sample lysis device;

3-prefabricating a sample to be detected;

31-a tube moving device;

311-liquid taking table;

3111-jaw six;

312-tube moving manipulator;

3121-X axis drive five;

3122-Y-axis drive five;

3123-Z-axis drive five;

3124-rotating jaw three;

32-a pipetting device;

321-a drip station;

322-consumable stock bin;

3221-eight connecting tubes;

3222-eight connecting covers;

3223-a pipetting cannula;

3224-cannula retrieval device;

323-pipetting robot;

3231-X axis drive six;

3232-Y axis drive six;

3233-Z axis drive six;

3234-grip jaw seven;

3235-pipette;

3236-grip jaw eight;

33-a capping device;

331-a capping station;

3311-Linear drive;

332-a capping machine;

4-PCR instrument;

5-test tube stock bin;

51-enrichment tube;

511-enrichment lid;

52-a lysis tube;

53-nucleic acid extraction tube;

6-lifting device.

Detailed Description

Embodiments of the present invention are described in detail below with reference to the accompanying drawings.

It should be noted that, in the case of no conflict, the features in the following embodiments and examples may be combined with each other; moreover, based on the embodiments in the present disclosure, all other embodiments obtained by a person of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present disclosure.

It is noted that various aspects of the embodiments are described below within the scope of the appended claims. It should be apparent that the aspects described herein may be embodied in a wide variety of forms and that any specific structure and/or function described herein is merely illustrative. Based on the disclosure, one skilled in the art should appreciate that one aspect described herein may be implemented independently of any other aspects and that two or more of these aspects may be combined in various ways. For example, an apparatus may be implemented and/or a method practiced using any number of the aspects set forth herein. Additionally, such an apparatus may be implemented and/or such a method may be practiced using other structure and/or functionality in addition to one or more of the aspects set forth herein.

It should be noted that the steps described below, the suffixed numbers of which are merely for distinguishing between different steps, and do not represent the order of the steps.

Fig. 1 is a schematic structural diagram of a complete machine to which the apparatus for prefabricating a sample to be examined according to this embodiment is applied, fig. 2 is a schematic structural diagram of fig. 1 with a mask removed from a rack 1, fig. 3 and fig. 4 are two schematic structural diagrams of fig. 2 with a bottom structure of the rack 1 removed, and fig. 5 and fig. 6 are two schematic structural diagrams of the apparatus for prefabricating a sample to be examined according to this embodiment, respectively.

As shown in the figure, the core of the apparatus 3 for preparing a specimen to be tested is a specimen pipetting and capping apparatus, which mainly comprises a tube-moving apparatus 31, a capping apparatus 33 and a pipetting apparatus 32 disposed on the rack 1.

The rear part of the frame 1 is also provided with a lifting device 6 in a closed way for conveying the test tube bin 5, and the test tube bin 5 is used for providing a cracking tube 52 with cracking liquid and a nucleic acid extracting tube 53 with a detected sample.

As shown in fig. 5 and 6, the apparatus 3 for preparing a specimen to be tested includes a tube moving device 31, a capping device 33 and a liquid transferring device 32, which are arranged in sequence from back to front, wherein:

the tube moving device 31 comprises a liquid taking table 311 and a tube moving manipulator 312 arranged above the liquid taking table 311, wherein the tube moving manipulator 312 is used for moving the nucleic acid extracting tube 53 filled with the lysis sample in the test tube bin 5 to the liquid taking table 311 and opening the cover; the capping device 33 is used for capping the eight-connected cap 3222 into the eight-connected tube 3221, the pipetting device 32 comprises a dripping table 321, a consumable material bin 322 and a pipetting manipulator 323 arranged thereon, and the pipetting manipulator 323 is used for transferring the eight-connected tube 3221 in the consumable material bin 322 to the dripping table 321, and taking liquid from the nucleic acid extraction tube 53 and dripping the liquid into the eight-connected tube 3221 to obtain a sample to be tested.

FIGS. 7 and 8 are two schematic views of the tube transfer device 31, and as shown in FIGS. 7 and 8, the liquid taking stand 311 of the tube transfer device 31 has a rack structure, and a gripper six 3111 for gripping the nucleic acid isolation tube 53 is provided at the lower part thereof. This tube moving manipulator 312 includes: an X-axis driver five 3121, a Y-axis driver five 3122, a Z-axis driver five 3123 and a rotating jaw three 3124, the X-axis driver five 3121 is used for driving it to move in the left-right direction of the rack 1, the Y-axis driver five 3122 is used for driving it to move in the front-rear direction of the rack 1, the Z-axis driver five 3123 is used for driving it to move in the up-down direction of the rack 1, and the rotating jaw three 3124 is used for holding the nucleic acid extraction tube 53 and rotating the lid.

In operation, the tube moving manipulator 312 moves the nucleic acid extraction tube 53 loaded with a lysed sample in the test tube bin 5 to the liquid taking table 311, the clamping jaw six 3111 of the liquid taking table 311 clamps the nucleic acid extraction tube 53, the rotating jaw three 3124 unscrews the enrichment cover 511 of the nucleic acid extraction tube 53 for the liquid moving device 3235 of the liquid moving manipulator 323 to take liquid, after the liquid taking is completed, the rotating jaw three 3124 covers and screws the tube cover of the nucleic acid extraction tube 53, the clamping jaw six 3111 loosens the nucleic acid extraction tube 53, and the tube moving manipulator 312 returns the nucleic acid extraction tube 53 to the test tube bin 5.

Fig. 9 and 10 are two schematic structural views of the pipetting device 32, and in fig. 10, the X-axis driver six 3231 and the Y-axis driver six 3232 of the pipetting device 32 are hidden, and a schematic structural view of the capping device 33 is shown. Referring to fig. 7, 8, 9 and 10, the consumable material bin 322 of the pipetting device 32 has a drawer-like structure, which can be pulled out of the rack 1 along the left-right direction of the rack 1 to replace the consumable material. The consumable comprises: an eight-connected tube 3221 for containing a sample to be tested taken out from the nucleic acid extraction tube 53; an eight-connecting cover 3222 for covering the eight-connecting pipe 3221; a pipette tip 3223 for pipetting a liquid from the nucleic acid isolation tube 53 into the eight-linked tube 3221. On the consumable stock bin 322, a cannula recovery device 3224 is also provided, the cannula recovery device 3224 is filled with a disinfectant for storing used pipette cannulas 3223.

The pipetting robot 323 of the pipetting device 32 includes an X-axis driver six 3231, a Y-axis driver six 3232, a Z-axis driver six 3233, a gripper seven 3234, a gripper eight 3236, and a pipettor 3235, wherein:

an X-axis driver six 3231 is used for driving the rack to move along the left-right direction of the rack 1, a Y-axis driver six 3232 is used for driving the rack to move along the front-back direction of the rack 1, a Z-axis driver six 3233 is used for driving the rack 1 to move along the up-down direction, a clamping jaw seven 3234 is used for moving the eight-connected tube 3221 to the dropping platform 321, a clamping jaw eight 3236 is used for moving the eight-connected cover 3222 to the eight-connected tube 3221, and a liquid transferring device 3235 is used for operating the liquid transferring sleeve 3223 to take liquid from the nucleic acid extracting tube 53 on the liquid taking platform 311 and drop the liquid into the eight-connected tube 3221 on the dropping platform 321.

The capping device comprises a capping table 331 and a capping machine 332, wherein the capping table 331 is used for placing an eight-connected pipe 3221 with eight-connected caps 3222, and a linear driver 3311 for driving the capping table 331 to move below the capping machine 332 is arranged at the lower part of the capping table 331; the capper 332 is used to press the eight-connected cap 3222 into the eight-connected tube 3221.

Specifically, in operation, the seven gripper 3234 of the pipetting robot 323 moves the eight-connected tube 3221 in the consumable material silo 322 to the dropping table 321, the pipettor 3235 picks up the liquid from the liquid-removing cannula 3223 in the test tube silo 5, the nucleic acid extraction tube 53 on the liquid-removing table 311 and drops the liquid into the eight-connected tube 3221 on the dropping table 321, after the liquid is completely removed, the eight gripper 3236 moves the eight-connected cap 3222 in the test tube silo 5 to the eight-connected tube 3221 on the dropping table 321, the six gripper 3111 moves the eight-connected tube 3221 covered with the eight-connected cap 3222 from the dropping table 321 to the capping table 331, the capping table 331 sends the eight-connected tube 3221 covered with the eight-connected cap 3222 to the lower side of the capping machine 332 through the linear driver 3311, the capping machine 332 presses down the eight-connected cap 3222, and the eight-connected cap 3222 is tightly pressed into the eight-connected tube 3221.

The pipette 3235 also discards the used pipette tip 3223 in the tip collection device 3224 after the completion of the liquid extraction.

As shown in fig. 1 and fig. 2, the apparatus for preparing a sample to be examined according to the present embodiment further includes an identification and verification device 13 and an isolation door device 14, wherein the identification and verification device 13 includes a code scanning device for identifying the barcode information on the enrichment tube 51, and/or an identity authentication device for identifying the identity information of the examinee, and/or an image recognition device 2114 for identifying whether the examinee is in normal operation. In this embodiment, all of the above-described identification/verification devices 13 are included, and in other embodiments, only one or two of them may be provided.

The isolation door device 14 is disposed in front of the sample enrichment device 21 for closing the sample enrichment device 21 to prevent the sample from being contaminated.

The device for preparing a sample to be detected in this embodiment further comprises a sterilizing device 12 for sterilizing before and after detection to prevent the sample to be detected from being polluted or leaked. In this embodiment, the killing device 12 is mainly a novel ultraviolet excimer intelligent 222 nm killing lamp, and is not only disposed on the lower layer of the rack 1, but also disposed on the upper layer of the rack 1 at a plurality of locations (not shown). In other embodiments, the killing device 12 may also include a high temperature steam killing apparatus, a disinfectant spraying apparatus, or the like.

In the nucleic acid detecting apparatus of this embodiment, the X-axis driver five 3121, the X-axis driver six 3231, the Y-axis driver five 3122, the Y-axis driver six 3232, the Z-axis driver five 3123, and the Z-axis driver six 3233 are all linear driving mechanisms, and the driving manner is an electric, pneumatic, or hydraulic driving manner, which is not described again in detail.

The embodiment also discloses a method for prefabricating a sample to be detected according to the device for prefabricating the sample to be detected, which mainly comprises a tube moving link, a liquid moving link and a gland link, wherein:

a tube moving step, in which the nucleic acid extraction tube 53 filled with the lysed sample in the test tube bin 5 is moved to the liquid extraction platform 311 and the cover is opened mainly by a tube moving manipulator 312;

a pipetting step of transferring the eight-linked tube 3221 in the consumable material bin 322 to the dripping table 321 mainly by the pipetting manipulator 323, and dripping the solution taken from the nucleic acid extracting tube 53 into the eight-linked tube 3221;

a capping link, namely, an eight-connecting cap 3222 is tightly capped into the eight-connecting pipe 3221 by a capping machine 332;

in the on-machine detection step, the pipette manipulator 323 sends the eight-connected tube 3221 covered with the eight-connected cover 3222 to the PCR instrument 4 for detection.

The pipe moving ring joint comprises the following steps:

firstly, the tube transferring manipulator 312 transfers the nucleic acid extracting tube 53 filled with the lysis sample in the test tube bin 5 to the liquid extracting platform 311;

step two, the clamping jaw six 3111 of the liquid-extracting table 311 clamps the nucleic acid extracting tube 53;

thirdly, the rotating claw 3124 of the tube moving manipulator 312 unscrews the enrichment cover 511 of the nucleic acid extraction tube 53;

step four, after the liquid extraction is completed, the third rotating claw 3124 of the tube moving manipulator 312 covers the tube cover of the nucleic acid extraction tube 53;

step five, the clamping jaw six 3111 of the liquid taking table 311 clamps the nucleic acid extracting tube 53, and the rotating jaw three 3124 drives the enrichment cover 511 to rotate so as to screw down the enrichment cover 511;

step six, the clamping jaw six 3111 of the liquid taking table 311 releases the nucleic acid extracting tube 53, and the tube transferring manipulator 312 returns the nucleic acid extracting tube 53 to the test tube bin 5.

The pipetting link comprises the following steps:

step one, the gripping claw seven 3234 of the pipetting manipulator 323 transfers the eight-connected pipe 3221 in the consumable material bin 322 to the dripping table 321;

a second step of loading a pipette 3223 from the test tube magazine 5 into the pipette 3235 of the pipette robot 323;

step three, the pipette 3235 of the pipette robot 312 operates the pipette 3223 to pipette the nucleic acid extracting tube 53 on the pipette table 311 and drop the nucleic acid extracting tube into the eight-linked tube 3221 on the dripping table 321;

in step four, the pipette 3235 of the pipette robot 312 drops the used pipette pot 3223 into the pot collection device 3224.

The capping link comprises the following steps:

firstly, the clamping jaw eight 3236 of the pipetting manipulator 323 moves an eight-connecting cover 3222 in the test tube bin 5 to an eight-connecting pipe 3221 positioned on the dropping platform 321;

step two, the gripper six 3111 of the pipetting robot 323 transfers the eight-connected tube 3221 covered with the eight-connected cover 3222 from the dropping table 321 to the capping table 331;

step three, the capping table 331 sends the eight-connected pipe 3221 capped with the eight-connected cap 3222 to the lower part of the capping machine 332 through the linear driver 3311;

step four, the capping machine 332 presses down to tightly cap the eight connecting caps 3222 into the eight connecting tubes 3221.

The prefabrication method of the sample to be detected further comprises an identification and verification link, and comprises the following steps:

step one, identifying the identity information of a detected person through an identity authentication device, opening an isolation door after the identity information passes the authentication, and prompting the detected person to put the enrichment pipe 51 into a placing table 211;

and step two, identifying the bar code information of the enrichment pipe 51 placed on the object placing table 211 through a code scanning device, closing the isolation door and giving a prompt after successful identification.

The method for prefabricating the sample to be detected further comprises a sterilization link, namely, before and after sample pretreatment, 3-way sterilization is carried out on the sample prefabricating device to be detected through at least one of ultraviolet rays, high-temperature steam and disinfectants.

Compared with the prior art, the prefabricating device and the prefabricating method for the sample to be detected have the following advantages that:

(1) Full-automatic mechanical operation saves medical resources, reduces professional's demand. The prefabricating device for the sample to be detected integrates a series of functions of tube moving, cover opening, liquid taking, liquid dropping, cover taking, cover pressing and the like of cracking the sample, is operated in a full-automatic and mechanical mode, can greatly reduce requirements of related professionals, saves medical resources, and reduces epidemic prevention cost.

(2) The operation of the closed space and complete sterilization measures avoid cross infection. The prefabricating device for the sample to be detected is completed in a relatively closed space in the frame, has a series of sterilization and disinfection measures such as high-temperature sterilization, ultraviolet lamp sterilization and the like which meet the national standard, can effectively prevent cross contamination, greatly improves epidemic situation prevention and control efficiency, and reduces the infection risk of people.

(3) The structure is compact, and multi-node, multi-level and quick deployment can be realized. The prefabricating device for the sample to be detected has the advantages of compact structure, small floor area, light weight and flexible arrangement, can form a full-automatic unmanned nucleic acid sampling and detecting system, has high system modularization degree, is extremely easy to deploy aiming at the existing complex, multi-level, distributed and emergent epidemic situation prevention and control requirements of domestic epidemic situations, can be rapidly popularized to urban communities, schools, gardens, hotels, construction sites, traffic entrances, large business supermans, urban and rural junctions and vast rural areas, and can build a strong barrier for basic epidemic situation prevention and control.

The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (17)

1. The utility model provides a ready-made device of sample of examining which characterized in that, includes the frame and sets up move the pipe device and move the liquid device on it:

the tube moving device comprises a test tube bin, a liquid taking platform and a tube moving manipulator arranged above the liquid taking platform,

the liquid-transfering device comprises a liquid-transfering platform, a consumable material bin and a liquid-transfering manipulator arranged on the liquid-transfering platform,

the tube moving manipulator is used for moving the nucleic acid extraction tube filled with the cracking sample in the test tube bin to the liquid taking table and opening the cover;

move liquid mechanical hand be used for with eight connecting tubes in the consumptive material feed bin are transferred to the dropping liquid platform, and follow it drips into to get liquid in the nucleic acid extraction tube eight connecting tubes obtain waiting to examine the sample.

2. The apparatus for preparing a sample to be tested according to claim 1, wherein the liquid-extracting stage is a rack structure, and a clamping jaw six for clamping the nucleic acid-extracting tube is provided at a lower portion thereof.

3. The apparatus for prefabricating a specimen to be examined according to claim 1, wherein the tube moving robot includes:

the X-axis driver V is used for driving the rack to move in the left-right direction;

a fifth Y-axis driver for driving the rack to move back and forth;

a Z-axis driver V for driving the rack to move up and down;

a third rotating claw for clamping the nucleic acid extracting tube and rotating the cover.

4. The apparatus for preparing a sample to be tested according to claim 1, wherein the consumable stock bin comprises at least one of the following consumables:

the eight-connecting pipe is used for containing a sample to be detected taken out from the nucleic acid extraction pipe;

the eight-connection cover is used for sealing the eight-connection pipe;

and the liquid-transferring sleeve is used for taking liquid from the nucleic acid extracting tube and dripping the liquid into the eight-connected tube.

5. The apparatus for preparing a sample to be examined according to claim 4, wherein the pipetting robot comprises:

the X-axis driver VI is used for driving the X-axis driver VI to move along the left and right directions of the rack;

a sixth Y-axis driver for driving the rack to move in the front-back direction;

a Z-axis driver VI for driving the rack to move up and down;

a clamping jaw seven for moving the eight-connected pipe to the dropping platform;

and the pipettor is used for operating the pipette sleeve to take liquid from the nucleic acid extraction tube on the liquid taking platform and dripping the liquid into the eight-connected tubes on the liquid dripping platform.

6. The apparatus for preparing a sample to be examined as claimed in claim 5, wherein the pipetting robot is further provided with a gripper eight for transferring the eight-link cap to an eight-link tube.

7. The apparatus for preparing a sample to be examined according to claim 6, further comprising a capping apparatus comprising:

the gland table is used for placing eight-connected pipes with eight-connected caps, and a linear driving mechanism for driving the gland table to move to the lower part of the capping machine is arranged at the lower part of the gland table;

and the capping machine is used for capping the eight-connection cap into the eight-connection pipe.

8. The apparatus for preparing a sample to be examined as claimed in claim 1 or 4, further comprising a cannula recovering device containing a disinfectant for storing used pipette cannulae.

9. The apparatus for preparing a sample to be tested according to claim 1, further comprising a sterilizing device for sterilizing the sample before and after the test to prevent the sample from being contaminated or leaked.

10. The apparatus of claim 4, wherein the consumable storage bin is a drawer-like structure that can be pulled out of the rack in a left-right direction of the rack to replace the consumable.

11. The prefabricating method for the sample to be detected is characterized by comprising a tube moving link, a liquid moving link and a gland link, wherein:

the tube moving ring mainly comprises the following actions that a nucleic acid extraction tube filled with a cracking sample in the test tube bin is moved to a liquid taking table through a tube moving manipulator, and the cover of the liquid taking table is opened;

the pipetting link mainly comprises the following actions of transferring eight connected tubes in the consumable material bin to a liquid dripping table through a pipetting manipulator, and taking liquid from the nucleic acid extraction tube and dripping the liquid into the eight connected tubes;

the capping step mainly comprises the following actions of capping eight-connected caps into the eight-connected tubes through a capping machine.

12. The method for prefabricating a specimen to be examined according to claim 11, wherein said tube moving collar comprises the steps of:

firstly, a tube moving manipulator moves a nucleic acid extraction tube filled with a lysis sample in a test tube bin to a liquid taking platform;

step two, clamping the nucleic acid extracting tube by a clamping jaw six of the liquid taking table;

and thirdly, unscrewing an enrichment cover of the nucleic acid extraction tube by a rotating claw III of the tube moving manipulator.

13. The method for prefabricating a specimen to be examined according to claim 12, wherein the tube moving ring section further comprises the steps of:

step four, after liquid taking is finished, covering the tube cover of the nucleic acid extraction tube by the rotating claw III of the tube moving manipulator;

fifthly, clamping the nucleic acid extraction tube by a clamping jaw six of the liquid taking table, and driving the enrichment cover to rotate by a rotating jaw three so as to screw down the enrichment cover;

and sixthly, loosening the nucleic acid extraction tube by using a clamping jaw six of the liquid taking platform, and sending the nucleic acid extraction tube back to the test tube bin by using the tube moving manipulator.

14. The method for preparing a sample to be examined as claimed in claim 11, characterized in that the pipetting step comprises the following steps:

moving eight connecting pipes in the consumable material bin to the liquid dropping platform by using a clamping jaw seven of the liquid transferring manipulator;

step two, a liquid transfer sleeve is arranged in the test tube bin by a liquid transfer machine of the liquid transfer manipulator;

and step three, operating the liquid-transferring sleeve to take liquid from the nucleic acid extracting tube on the liquid-taking platform and dripping the liquid into the eight-connected tubes on the liquid-dropping platform by the liquid-transferring manipulator of the pipe-transferring manipulator.

15. The method for preparing a specimen to be examined, according to claim 14, characterized in that the pipetting step further comprises the steps of:

and fourthly, throwing the used pipetting sleeve into the sleeve recycling device by a pipettor of the pipette manipulator.

16. The method for prefabricating a specimen to be inspected according to claim 11, wherein the capping link comprises the steps of:

moving an eight-connection cover in the test tube bin to an eight-connection tube positioned on the liquid dropping platform by using a clamping jaw eight of the liquid transferring manipulator;

step two, the clamping jaw six of the liquid-transfering manipulator transfers the eight-connected pipes covered with the eight-connected covers to a pressing platform from the liquid-dropping platform;

step three, the capping table conveys the eight-connected pipes covered with eight-connected caps to the lower part of the capping machine through a linear driving mechanism;

and step four, pressing down by the capping machine, and capping the eight-connection cap into the eight-connection pipe.

17. The method for preparing a specimen to be examined according to claim 11, further comprising a sterilizing step of sterilizing the specimen preparation means to be examined by at least one of ultraviolet rays, high-temperature steam and a disinfectant before and after the preparation of the specimen to be examined.

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