CN115869341A - Application of earthworm extract in preparation of anti-fatigue product, and medicine, health product and food using earthworm extract - Google Patents

Abstract

The invention discloses an application of earthworm extract in preparing an anti-fatigue product. The earthworm extract extracted from Eisenia foetida by adopting an organic solvent reflux method is independently used for playing the anti-fatigue effect. The earthworm extract can obviously prolong the weight swimming time of mice, can obviously reduce the content of blood lactic acid and urea nitrogen, can improve the content of hepatic glycogen, has better anti-fatigue effect, is suitable for preparing anti-fatigue medicaments, health-care products or foods, and researches the new application of the earthworm extract in the field of preparing anti-fatigue medicaments, health-care products or foods.

Description

Application of earthworm extract in preparation of anti-fatigue product, and medicine, health product and food using earthworm extract

Technical Field

The invention belongs to the technical field of application of earthworm extracts, and particularly relates to application of earthworm extracts in preparation of anti-fatigue products, and medicines, health-care products and foods using the earthworm extracts.

Background

With the acceleration of working pressure and life rhythm, more and more people have fatigue symptoms such as dizziness, drowsiness, fatigue and the like. Delaying the occurrence of fatigue and promoting the recovery of fatigue are research hotspots of military medicine and sports medicine. At present, measures and medicines for fatigue cannot achieve ideal effects. Although some chemical drugs can achieve certain anti-fatigue effect, the components of the chemical drugs are similar to those of stimulants, and the long-term administration of the chemical drugs can cause certain damage to the body. The natural medicine can exert special curative effect due to the unique structure, and the screening of anti-fatigue from the natural medicine becomes the hotspot of the current research.

Eiseniafoetida (Eiseniafoetida) belonging to the family Lumbriaceae, also known as red earthworm and earthworm. Because of high reproduction rate and strong adaptability, the earthworm breeding method is the most common earthworm improved variety in the world at present. In recent years, eisenia fetida has increasingly wide application in the pharmaceutical industry. Researches find that Eisenia foetida has the effects of clearing heat, arresting convulsion, relieving asthma, promoting urination, resisting blood coagulation, thrombolysis, resisting radiation, resisting tumors and the like, and is applied to the treatment of diseases such as coronary heart disease, diabetes, hypertension, cerebral hemorrhage, malignant tumors and the like. However, studies on the anti-fatigue effect of Eisenia foetida, i.e., pheretima, have not been found.

Through searching, no patent publication related to the present patent application has been found.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides the application of the earthworm extract in preparing anti-fatigue products and the medicines, health-care products and foods utilizing the extract.

The technical scheme adopted by the invention for solving the technical problems is as follows:

application of Lumbricus extract in preparing antifatigue product is provided.

Further, the earthworm extract is obtained by extracting Eisenia foetida by an organic solvent reflux method.

Further, the anti-fatigue is an ability to resist sports fatigue.

Further, the application is the application of the earthworm extract as an anti-fatigue effective component in anti-fatigue medicines, health products or foods.

Further, the earthworm extract is used independently or in combination with an anti-fatigue component other than the earthworm extract.

Furthermore, the medicine, the health-care product or the food is the medicine, the health-care product or the food for reducing the content of serum urea nitrogen and lactic acid and improving the content of hepatic glycogen.

Furthermore, the dosage form of the medicine is granule, tablet, capsule or decoction;

the health care product is oral liquid, tablets and capsules;

the food is liquid beverage, cookies, rice water.

Further, the preparation steps of the earthworm extract are as follows:

step one, treating fresh earthworms, namely Eisenia foetida:

(1) Washing fresh Lumbricus with clear water to remove impurities on Lumbricus body surface, and washing mucus on Lumbricus body surface with warm water;

(2) Soaking Lumbricus in 20-30% saline water for 2-3 hr, purifying impurities in body, and washing off saline water on body surface;

(3) Cutting cleaned Lumbricus into segments, spreading, sun drying, and making into dried Lumbricus;

step two, preparing an organic solvent:

pouring petroleum ether, chloroform and methanol in a mass ratio of 2;

step three, reflux extraction:

(1) Adding organic solvent into dried Lumbricus until the organic solvent overflows dried Lumbricus by 10-15mm, heating with water bath at 45 deg.C, extracting by reflux extraction for 30min to obtain extractive solution;

(2) Pouring out the extraction mixed solution, adding the organic solvent again, repeating the reflux extraction operation, and performing reflux extraction for 5 times;

step four, recovering the organic solvent:

heating the extracted mixed solution by using a water bath method for distillation, setting the heating temperature to 65 ℃, collecting the evaporated organic solvent until the mixed solution is viscous, and stopping distillation after fire is turned off;

step five, concentration and drying:

(1) Heating and evaporating the mixed solution by using a water bath method, setting the heating temperature to be 58 ℃, and continuously stirring in the evaporation process until the mixed solution is in a thick paste shape;

(2) Vacuum drying the mixed liquid of the thick paste, setting the working temperature of the vacuum drying to be 39-42 ℃, working for 10min, then adjusting the temperature to be 45-50 ℃, and continuously drying for 30min to prepare a solid earthworm extract;

the earthworm extract prepared has a protein content of >50%.

An anti-fatigue product using earthworm extract, which is a medicament, oral liquid or liquid beverage, is prepared by the following steps:

a medicine prepared from earthworm extract is prepared by the following steps:

weighing lactose, sugar and earthworm extract, starch slurry with the mass concentration of 10% and magnesium stearate, weighing the lactose and the sugar according to the prescription amount, adding into a high-speed mixing granulator, dry-mixing for 1-2 minutes, adding the starch slurry, controlling the temperature at 30-50 ℃, and wet-mixing to prepare blank granules; then making the earthworm extract into an ethanol solution with the mass concentration of 10 percent, stirring the ethanol solution into blank granules, sieving, drying, mixing with magnesium stearate uniformly, and tabletting; wherein, the mass ratio of lactose, sugar, earthworm extract, starch slurry with the mass concentration of 10 percent and magnesium stearate is 88.8:38.0:20.0:10:1.0g;

an oral liquid prepared from Lumbricus extract is prepared by the following steps:

weighing earthworm extract, codonopsis pilosula, longan, red date, donkey-hide gelatin and mulberry, decocting for 2 hours, filtering, concentrating the filtrate until the relative density is 1.21, adding ethanol until the alcohol content reaches 65-70%, refrigerating for 48 hours, filtering, recovering the ethanol, adding 0.5% of activated carbon, heating for 30 minutes, filtering, adding water, filtering, encapsulating and sterilizing to obtain the traditional Chinese medicine composition; wherein, the earthworm extract: codonopsis pilosula: longan: red dates: e, donkey-hide gelatin: mulberry fruit: the mass ratio of water is as follows: 20:5:5:5:3:1:1000, parts by weight;

a liquid beverage containing Lumbricus extract is prepared by the following steps:

mixing Lumbricus extract, white sugar, citric acid, sodium citrate, lemon yellow, essence, and potassium sorbate, stirring Lumbricus extract in 5-10 times of boiling water for 20min, filtering, adding white sugar into the filtrate for flavoring, dissolving citric acid in hot water, adding citric acid solution, controlling the temperature of the solution below 40 deg.C, adding lemon yellow, essence, potassium sorbate and water, stirring, homogenizing under 8-20 MPa for 2 times to obtain a uniform mixed solution, bottling, sterilizing, and cooling; wherein, the mass ratio of the earthworm extract, the white granulated sugar, the citric acid, the sodium citrate, the lemon yellow, the essence, the potassium sorbate and the water is 20.0:2:1.5:1:0.5:0.5:0.3:5000.

the invention has the advantages and positive effects that:

1. the earthworm extract extracted from Eisenia foetida by adopting an organic solvent reflux method is independently used for playing the anti-fatigue effect. The earthworm extract contains 50-60% of protein. The earthworm extract can obviously prolong the weight swimming time of mice, can obviously reduce the blood lactic acid content and the urea nitrogen content, can improve the liver glycogen content, has better anti-fatigue effect, is suitable for preparing anti-fatigue medicaments, health-care products or foods, and researches the new application of the earthworm extract in the field of preparing anti-fatigue medicaments, health-care products or foods.

Drawings

FIG. 1 is a graph showing the change in body weight of mice after 30 days of administration in the present invention;

FIG. 2 is a graph showing the effect of Lumbricus extract on the swimming time of mice;

FIG. 3 is a graph showing the effect of Lumbricus extract on urea nitrogen content in mice;

FIG. 4 is a graph showing the effect of Lumbricus extract on liver glycogen changes in mice in accordance with the present invention;

FIG. 5 is a graph showing the effect of the Lumbricus extract on blood lactate content of mice.

Detailed Description

The following detailed description of the embodiments of the present invention is provided for the purpose of illustration and not limitation, and should not be construed as limiting the scope of the invention.

The raw materials used in the invention are conventional commercial products unless otherwise specified; the methods used in the present invention are, unless otherwise specified, conventional in the art.

Application of Lumbricus extract in preparing antifatigue product is provided.

Preferably, the earthworm extract is obtained by extracting Eisenia foetida with an organic solvent reflux method.

Preferably, the anti-fatigue is an anti-exercise fatigue capability.

Preferably, the application is the application of the earthworm extract as an anti-fatigue effective component in the aspects of anti-fatigue medicines, health-care products or foods.

Preferably, the earthworm extract is used independently or in combination with an anti-fatigue component other than the earthworm extract.

Preferably, the medicine, health-care product or food is a medicine, health-care product or food for reducing the content of serum urea nitrogen and lactic acid and increasing the content of hepatic glycogen.

Preferably, the dosage form of the medicine is granule, tablet, capsule or decoction;

the health care product is oral liquid, tablets and capsules;

the food is liquid beverage, cookies, rice water.

Preferably, the preparation method of the earthworm extract comprises the following steps:

step one, treating fresh earthworms, namely Eisenia fetida:

(1) Cleaning fresh Lumbricus with clear water to remove impurities on Lumbricus body surface, and washing mucus on Lumbricus body surface with warm water;

(2) Soaking Lumbricus in 20-30% saline water for 2-3 hr, purifying impurities in body, and washing off saline water on body surface with clear water;

(3) Cutting cleaned Lumbricus into segments, spreading, sun drying, and making into dried Lumbricus;

step two, preparing an organic solvent:

pouring petroleum ether, chloroform and methanol in a mass ratio of 2;

step three, reflux extraction:

(1) Adding organic solvent into dried Lumbricus until the organic solvent is 10-15mm over dried Lumbricus, heating with water bath at 45 deg.C, extracting by reflux extraction for 30min to obtain extractive solution;

(2) Pouring out the extraction mixed solution, adding the organic solvent again, repeating the reflux extraction operation, and performing reflux extraction for 5 times;

step four, recovering the organic solvent:

heating the extraction mixed solution by using a water bath method for distillation, setting the heating temperature to 65 ℃, collecting the evaporated organic solvent until the mixed solution is viscous, and stopping distillation after fire is cut off;

step five, concentration and drying:

(1) Heating and evaporating the mixed solution by using a water bath method, wherein the heating temperature is set to 58 ℃, and continuously stirring in the evaporation process until the mixed solution is in a thick paste shape;

(2) Vacuum drying the mixed liquid of the thick paste, setting the working temperature of the vacuum drying to be 39-42 ℃, working for 10min, then adjusting the temperature to be 45-50 ℃, and continuously drying for 30min to prepare a solid earthworm extract;

the earthworm extract prepared has a protein content of >50%.

An anti-fatigue product using earthworm extract, which is a medicament, oral liquid or liquid beverage, is prepared by the following steps:

a medicine prepared from earthworm extract is prepared by the following steps:

weighing lactose, sugar and earthworm extract, starch slurry with the mass concentration of 10% and magnesium stearate, weighing the lactose and the sugar according to the prescription amount, adding into a high-speed mixing granulator, dry-mixing for 1-2 minutes, adding the starch slurry, controlling the temperature at 30-50 ℃, and wet-mixing to prepare blank granules; then making the earthworm extract into an ethanol solution with the mass concentration of 10 percent, stirring the ethanol solution into blank granules, sieving, drying, mixing with magnesium stearate uniformly, and tabletting; wherein, the mass ratio of lactose, sugar, earthworm extract, starch slurry with the mass concentration of 10 percent and magnesium stearate is 88.8:38.0:20.0:10:1.0g;

an oral liquid prepared from Lumbricus extract is prepared by the following steps:

weighing earthworm extract, codonopsis pilosula, longan, red date, donkey-hide gelatin and mulberry, decocting for 2 hours, filtering, concentrating the filtrate until the relative density is 1.21, adding ethanol until the ethanol content reaches 65-70%, refrigerating for 48 hours, filtering, recovering the ethanol, adding 0.5% of activated carbon, heating for 30 minutes, filtering, adding water, filtering, encapsulating and sterilizing to obtain the traditional Chinese medicine composition; wherein, the earthworm extract: codonopsis pilosula: longan: red dates: e, donkey-hide gelatin: and (3) mulberry fruit: the mass ratio of water is as follows: 20:5:5:5:3:1:1000;

a liquid beverage containing Lumbricus extract is prepared by the following steps:

mixing Lumbricus extract, white sugar, citric acid, sodium citrate, lemon yellow, essence, and potassium sorbate, stirring Lumbricus extract in 5-10 times of boiling water for 20min, filtering, adding white sugar into the filtrate for flavoring, dissolving citric acid in hot water, adding citric acid solution, controlling the temperature of the solution below 40 deg.C, adding lemon yellow, essence, potassium sorbate and water, stirring, homogenizing under 8-20 MPa for 2 times to obtain a uniform mixed solution, bottling, sterilizing, and cooling; wherein, the mass ratio of the earthworm extract, the white granulated sugar, the citric acid, the sodium citrate, the lemon yellow, the essence, the potassium sorbate and the water is 20.0:2:1.5:1:0.5:0.5:0.3:5000.

specifically, the preparation and detection examples are as follows:

example 1:

the invention adopts an organic solvent reflux extraction method to extract and prepare the earthworm extract, and the specific preparation steps are as follows:

step one, treating fresh earthworms, namely Eisenia fetida:

(1) Washing fresh Lumbricus with clear water to remove impurities on Lumbricus body surface, and washing mucus on Lumbricus body surface with warm water;

(2) Soaking Lumbricus in 20-30% saline water for 2-3 hr, purifying impurities in body, and washing off saline water on body surface with clear water;

(3) Cutting cleaned Lumbricus into segments, spreading, sun drying, and making into dried Lumbricus;

step two, preparing an organic solvent:

pouring petroleum ether, chloroform and methanol in a mass ratio of 2;

step three, reflux extraction:

(1) Putting 200g of dried earthworms into a flask, adding an organic solvent until the organic solvent overflows the dried earthworms by 10-15mm, heating the earthworms by using a water bath method at 45 ℃, extracting the earthworms by using a reflux extraction method for 30min to obtain an extraction mixed solution;

(2) Pouring out the extraction mixed solution in the flask, adding the organic solvent again, repeating the reflux extraction operation, and performing reflux extraction for 5 times;

step four, recovering the organic solvent:

pouring the extraction mixed solution into a flask, heating by using a water bath method for distillation, setting the heating temperature to 65 ℃, collecting the evaporated organic solvent until the mixed solution in the flask is viscous, and stopping distillation after fire is cut off;

step five, concentration and drying:

(1) Pouring the mixed solution in the flask into a beaker, heating and evaporating by using a water bath method, wherein the heating temperature is set to be 58 ℃, and continuously stirring in the evaporation process until the mixed solution is in a thick paste shape;

(2) And putting the mixed liquid in the thick paste state into a low-temperature freeze drying chamber for vacuum drying, setting the working temperature of a vacuum drying box to be 39-42 ℃, working for 10min, then adjusting the temperature to be 45-50 ℃, and continuously drying for 30min to prepare the solid earthworm extract.

The characteristics of the earthworm extract obtained are as follows:

determination of protein content: protein content of the earthworm extract was determined using BCA method:

preparation before detection: preparation of protein standard and preparation of BCA working solution according to requirements of kit

Drawing a standard curve:

taking 0, 1, 2, 4, 8, 12, 16 and 20 mu L of standard substance, adding PBS solution to make up to 20 mu L, adding 200 mu LBCA working solution, and standing at room temperature for 2 hours. The blank was distilled water, 3 replicates of each group were measured for absorbance at 562nm and plotted as a standard curve. The regression equation is y =1.2012x +0.0092, r =0.9960.

Sample placement and detection

Taking 5 groups of Lumbricus extracts of different batches, preparing at concentration of 0.5mg/mL, taking 20 μ L of each sample, adding 200 μ LBCA working solution, standing at room temperature for 2 hr, measuring absorbance at 562nm, and fitting the obtained result into standard curve, wherein the Lumbricus extract has protein content of 50-60%.

Example 2

The application of the earthworm extract in resisting fatigue comprises the following steps:

experimental grouping and dosing:

SPF KM male mice 48, weight 18-22 g, randomly divided into 4 groups of 12 mice each. Setting a negative control group (physiological saline), a low dosage group of earthworm extract 0.25g/kg/d, a medium dosage group of earthworm extract 0.5g/kg/d, a high dosage group of earthworm extract 1g/kg/d, and a dosage volume of 0.1mL/10g, and continuously administering for 30 days.

Data from the experiment were processed through SPSS25.0 software, one-way anova data.

Example 3

The application of the earthworm extract in resisting fatigue comprises the following steps:

after 30 days of gastric lavage, the results are shown in table 1 and figure 1, and compared with the control group, the weight of the administration group has no significant difference, which indicates that the earthworm extract has no toxic effect on mice.

Table 1: effect of Pheretima extract on weight change in mice

Example 4

Mouse weight swimming experiment:

in test group one, 30min after the last administration, the mice were loaded with a 5% weight lead skin on their tails and swim in a swimming pool (about 50 cm. Times.50 cm. Times.40 cm in size). The water depth is not less than 30cm, the water temperature is 25 +/-5.0 ℃, and the time from the beginning of swimming to the time when the mouse does not float out of the water surface within 8s, namely the time of swimming under load of the mouse, is recorded. The results are shown in table 2 and fig. 2, and the weight-bearing swimming time of the administration group is prolonged compared with the blank group, wherein the weight-bearing swimming time of the dosage group in the earthworm extract is the longest, which indicates that the earthworm extract can effectively prolong the swimming endurance of the mice.

Table 2: influence of Lumbricus extract on mouse weight bearing swimming time

Note: a, b, c represent the difference between the different groups

Example 5

And (3) measuring the urea nitrogen content:

in the second experiment group, 30min after the last administration, the patient swims in the swimming box for 90min without load, and after 60min of rest, the whole blood is collected by pulling out the eyeball (0.5 mL is added with EDTA anticoagulant). Placing in a refrigerator at 4 deg.C for about 3h, centrifuging at 2000r/min for 15min after blood coagulation, and collecting serum for use. The determination method is according to the kit instruction, and the kits are purchased from Nanjing to build the bioengineering institute. As shown in table 3 and fig. 3, the middle and high dose of the earthworm extract reduced the urea nitrogen content in the blood compared to the blank group, while the medium dose group showed the lowest urea nitrogen concentration, indicating that the medium dose can more effectively slow down the metabolic rate of the protein, thereby reducing or improving fatigue.

Table 3: effect of Pheretima extract on Urea Nitrogen content in mice

Example 6

Liver glycogen content determination:

and in the third experiment group, the liver is immediately killed 30min after the last administration, 100mg of the liver is accurately weighed by using filter paper after being rinsed by physiological saline, 4mL of trichloroacetic acid is added, each tube is homogenized for 50s, the operation is repeated for three times, the homogenate is poured into a centrifuge tube, and the centrifuge tube is centrifuged for 15min at 3000r/min after being kept stand for 10min. And (3) putting 1mL of supernatant into a 10mL centrifuge tube, adding 4mL of 95% ethanol into each tube, and fully and uniformly mixing until no interface is left between the two liquids. The mixture was stoppered with a clean stopper and allowed to stand overnight at room temperature. After the precipitation was complete, the tube was centrifuged at 3000r/min for 15min. Carefully pour off the supernatant and place the tube upside down for 10min. Liver glycogen content was determined by the anthrone method. The results are shown in table 4 and fig. 4, and the liver glycogen content was increased in the administered group compared to the blank group, indicating that the earthworm extract could improve liver glycogen storage.

Table 4: effect of earthworm extract on mouse liver glycogen changes

Note: a, b, c represent the difference between the different groups

Example 7

And (3) blood lactic acid content determination:

in the fourth experimental group, after 30min of the last administration, 0.1mL of orbital venous plexus blood of the mice was taken, 0.6mL of protein precipitator was added, and then the mice were swim in the swimming box for 10min. 0.1mL of blood is taken from the orbital venous plexus of the mouse at 0min and 20min after swimming, and 0.6mL of protein precipitant is added. The measurement method is described in the kit instruction. The area under the blood lactic acid curve is calculated as follows: area under the blood lactic acid curve =5 × (blood lactic acid value before swimming + blood lactic acid value 0min after 3 × blood lactic acid value after swimming + blood lactic acid value after 20min after 2 × resting after swimming) the results are shown in table 5 and fig. 5, and the mice in the earthworm extract group all had a different reduction in the area under the blood lactic acid curve compared to the blank group, and the reduction in lactic acid accumulation could delay the onset of fatigue or accelerate the recovery from fatigue.

Table 5: effect of earthworm extract on blood lactic acid content of mice

/>

Example 8

Organ index detection:

all the four groups of mice adopt cervical dislocation for post-mortem dissection to take out the heart, the liver, the spleen and the lung, the postrenal physiological saline is rinsed, and the filter paper sucks off the surface water and then weighs the mice. Organ index calculation method: organ index (%) = organ mass/body weight × 100%. The organ coefficient is an important index for reflecting the development of animal organs, and can visually reflect the toxic action of a test object on animals. As shown in Table 6, the results of the treatment groups were not significantly different from those of the control group (P > 0.05). This indicates that the earthworm extract does not cause organic lesions in mice.

TABLE 6 Lumbricus extract to mouse organ systemInfluence of the number

Example 9

A medicine prepared from Lumbricus extract is prepared by the following steps:

weighing 88.8g of lactose, 38.0g of sugar, 20.0g of earthworm extract, 10g of starch slurry with the mass concentration of 10% and 1.0g of magnesium stearate, weighing the lactose and the sugar according to the formula amount, adding the lactose and the sugar into a high-speed mixing granulator, dry-mixing for 1-2 minutes, adding the 10% starch slurry, controlling the temperature at 30-50 ℃, wet-mixing to prepare blank granules, preparing the earthworm extract into a 10% ethanol solution, stirring in the blank granules, sieving, drying, mixing with the magnesium stearate uniformly, and tabletting.

Example 10

An oral liquid prepared from Lumbricus extract is prepared by the following steps:

weighing 20.0g of earthworm extract, 5g of codonopsis pilosula, 5g of longan, 5g of red date, 3g of donkey-hide gelatin and 1g of mulberry, decocting for 2 hours, filtering, concentrating the filtrate until the relative density is 1.21, adding ethanol until the ethanol content reaches 65-70%, refrigerating for 48 hours, filtering, recovering the ethanol, adding 0.5% of activated carbon, heating for 30 minutes, filtering, adding water to 1000ml, filtering, encapsulating and sterilizing to obtain the traditional Chinese medicine composition.

Example 11

A liquid beverage containing Lumbricus extract is prepared by the following steps:

earthworm extract 20.0g, white granulated sugar 2g, citric acid 1.5g, sodium citrate 1g, lemon yellow 0.5g, essence 0.5g, potassium sorbate 0.3g, adding earthworm extract into boiling water 5-10 times of the earthworm extract, stirring for 20min, filtering, taking filtrate, adding white granulated sugar for seasoning, adding citric acid after being dissolved in hot water, controlling the temperature of feed liquid to be below 40 ℃, adding lemon yellow, essence, potassium sorbate and 5L of water, stirring uniformly, homogenizing for 2 times at 8-20 MPa to make the whole mixed liquid uniform, filling, sterilizing and cooling.

Although the embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, alterations and modifications are possible without departing from the spirit and scope of this disclosure and appended claims, and accordingly, the scope of this disclosure is not limited to the embodiments disclosed.

Claims (9)

1. Application of Lumbricus extract in preparing antifatigue product is provided.

2. Use according to claim 1, characterized in that: the earthworm extract is obtained by extracting Eisenia foetida with an organic solvent reflux method.

3. Use according to claim 1, characterized in that: the anti-fatigue is the ability to resist sports fatigue.

4. Use according to claim 1, characterized in that: the application is the application of the earthworm extract as an anti-fatigue effective component in the aspects of anti-fatigue medicines, health products or foods.

5. Use according to claim 4, characterized in that: the Lumbricus extract is used independently or in combination with anti-fatigue components other than Lumbricus extract.

6. Use according to claim 4, characterized in that: the medicine, health-care product or food can reduce the content of serum urea nitrogen and lactic acid and improve the content of hepatic glycogen.

7. Use according to claim 4, characterized in that: the dosage form of the medicine is granule, tablet, capsule or decoction;

the health care product is oral liquid, tablets and capsules;

the food is liquid beverage, cookies, rice water.

8. Use according to any one of claims 1 to 7, characterized in that: the preparation steps of the earthworm extract are as follows:

step one, treating fresh earthworms, namely Eisenia foetida:

(1) Washing fresh Lumbricus with clear water to remove impurities on Lumbricus body surface, and washing mucus on Lumbricus body surface with warm water;

(2) Soaking Lumbricus in 20-30% saline water for 2-3 hr, purifying impurities in body, and washing off saline water on body surface;

(3) Cutting cleaned Lumbricus into segments, spreading, sun drying, and making into dried Lumbricus;

step two, preparing an organic solvent:

pouring petroleum ether, chloroform and methanol in a mass ratio of 2;

step three, reflux extraction:

(1) Adding organic solvent into dried Lumbricus until the organic solvent overflows dried Lumbricus by 10-15mm, heating with water bath at 45 deg.C, extracting by reflux extraction for 30min to obtain extractive solution;

(2) Pouring out the extraction mixed solution, adding the organic solvent again, repeating the reflux extraction operation, and performing reflux extraction for 5 times;

step four, recovering the organic solvent:

heating the extracted mixed solution by using a water bath method for distillation, setting the heating temperature to 65 ℃, collecting the evaporated organic solvent until the mixed solution is viscous, and stopping distillation after fire is turned off;

step five, concentration and drying:

(1) Heating and evaporating the mixed solution by using a water bath method, wherein the heating temperature is set to 58 ℃, and continuously stirring in the evaporation process until the mixed solution is in a thick paste shape;

(2) Vacuum drying the mixed liquid of the thick paste, setting the working temperature of the vacuum drying to be 39-42 ℃, working for 10min, then adjusting the temperature to be 45-50 ℃, and continuously drying for 30min to prepare a solid earthworm extract;

the earthworm extract prepared has a protein content of more than 50%.

9. An anti-fatigue product using earthworm extract, which is characterized in that: the anti-fatigue product is a medicament, oral liquid or liquid beverage, and the preparation steps are as follows:

a medicine prepared from Lumbricus extract is prepared by the following steps:

weighing lactose, sugar and earthworm extract, starch slurry with the mass concentration of 10% and magnesium stearate, weighing the lactose and the sugar according to the prescription amount, adding into a high-speed mixing granulator, dry-mixing for 1-2 minutes, adding the starch slurry, controlling the temperature at 30-50 ℃, and wet-mixing to prepare blank granules; then making the earthworm extract into an ethanol solution with the mass concentration of 10 percent, stirring the ethanol solution into blank granules, sieving, drying, mixing with magnesium stearate uniformly, and tabletting; wherein the mass ratio of lactose, sugar, earthworm extract, starch slurry with the mass concentration of 10 percent and magnesium stearate is 88.8:38.0:20.0:10:1.0g;

an oral liquid prepared from Lumbricus extract is prepared by the following steps:

weighing earthworm extract, codonopsis pilosula, longan, red date, donkey-hide gelatin and mulberry, decocting for 2 hours, filtering, concentrating the filtrate until the relative density is 1.21, adding ethanol until the alcohol content reaches 65-70%, refrigerating for 48 hours, filtering, recovering the ethanol, adding 0.5% of activated carbon, heating for 30 minutes, filtering, adding water, filtering, encapsulating and sterilizing to obtain the traditional Chinese medicine composition; wherein, the earthworm extract: codonopsis pilosula: longan: red dates: e, donkey-hide gelatin: mulberry fruit: the mass ratio of water is as follows: 20:5:5:5:3:1:1000;

a liquid beverage containing Lumbricus extract is prepared by the following steps:

mixing Lumbricus extract, white sugar, citric acid, sodium citrate, lemon yellow, essence, and potassium sorbate, stirring Lumbricus extract in 5-10 times of boiling water for 20min, filtering, adding white sugar into the filtrate for flavoring, dissolving citric acid in hot water, adding citric acid solution, controlling the temperature of the solution below 40 deg.C, adding lemon yellow, essence, potassium sorbate and water, stirring, homogenizing under 8-20 MPa for 2 times to obtain a uniform mixed solution, bottling, sterilizing, and cooling; wherein, the mass ratio of the earthworm extract, the white granulated sugar, the citric acid, the sodium citrate, the lemon yellow, the essence, the potassium sorbate and the water is 20.0:2:1.5:1:0.5:0.5:0.3:5000.

Images