CN115845587A - Indole odor inhibitor - Google Patents

Abstract

The invention discloses an indole peculiar smell inhibitor, which comprises ester spice and a solvent, wherein the mass fraction of the ester spice is 0.01-5%; wherein the ester perfume is one or more of ethyl acetate, linalyl acetate, benzyl acetate, geranyl acetate, vanillyl acetate, ethyl acetoacetate, methyl dihydrojasmonate, propyl decalactone, butyl butyryl butyl lactate and o-tert-butylcyclohexyl acetate. The indole odor inhibitor with the structure can effectively reduce the perception degree of indole and/or 3-methylindole by people. Thereby achieving the purpose of odor inhibition.

Description

Indole odor inhibitor

Technical Field

The invention relates to the technical field of deodorant, aromatic and peculiar smell inhibition, in particular to an indole peculiar smell inhibitor.

Background

Malodorous gases produced in toilets, sewage treatment plants, landfills and some fermentation plants. Malodour has become one of seven public hazards in the world as an environmental pollution. The national standard GB14554-93 defines malodour as: all the gas substances which can stimulate olfactory organs to cause unpleasantness and damage living environment. The main components are as follows: nitrogen-containing compounds such as ammonia, sulfur-containing compounds such as hydrogen sulfide and mercaptans, and aldehydes, ketones, fatty acids, unsaturated hydrocarbons, and the like. The indole and 3-methylindole in the nitrogen-containing compounds are widely present in human and other mammal excreta and are particularly not easy to remove due to their chemical stability. Indole and 3-methylindole have very low odor thresholds and still have strong odor at lower concentrations. The odor represented by indole and 3-methylindole has a large influence on daily life.

In the prior art, a Chinese patent application CN201810175972.2A discloses an oxidation reaction tower for centralized treatment of indole and 3-methylindole. However, since the adsorption oxidation method is used to treat high-concentration offensive odor, the equipment is complicated, and the cost is high, so that the adsorption oxidation method cannot be applied to small-scale scenes such as households and public toilets. The Chinese patent application CN201711024991.7A discloses a toilet purification device, which utilizes an exhaust fan and a nozzle, wherein the exhaust fan is used for sucking odor of a toilet stool, the nozzle is arranged in a water tank and is used for spraying water, the exhaust fan is used for extracting the odor and mixing the odor with the water sprayed by the nozzle, and indole and skatole in the odor are transferred in the water to form clean air. This method is feasible, but since indole is soluble only in hot water and 3-methylindole is insoluble in water, its deodorizing effect is questionable.

In addition, many daily chemical manufacturers have developed toilet deodorizers, fragrances and the like containing perfume and essence aiming at the household application scene, and products for covering the peculiar smells of indole and 3-methylindole by using the strong fragrance of the perfume or the essence. However, these products have high requirements on the fragrance concentration, and the odor of indole and 3-methylindole is perceived while the final user feels the fragrance, so that the actual use effect is not obvious.

Disclosure of Invention

The invention aims to provide an indole peculiar smell inhibitor which can effectively reduce the perception degree of indole and/or 3-methylindole of people. Thereby achieving the purpose of odor inhibition.

In order to achieve the purpose, the invention provides an indole odor inhibitor, which comprises ester perfume and a solvent, wherein the mass fraction of the ester perfume is 0.01-5%;

wherein the ester perfume is one or more of ethyl acetate, linalyl acetate, benzyl acetate, geranyl acetate, vanillyl acetate, ethyl acetoacetate, methyl dihydrojasmonate, propyl decalactone, butyl butyryl butyl lactate and o-tert-butylcyclohexyl acetate.

Preferably, the solvent is a mixture of ethanol and water.

Preferably, the mass fraction of the ester perfume is 0.01-1%.

More preferably, the mass fraction of the ester-based perfume is 0.5%.

A preparation method of an indole odor inhibitor comprises dissolving the above ester perfume in the solvent, mixing well, and making into spray.

Preferably, the odor inhibitor is used for removing indole odor.

Preferably, the odor inhibitor is used to deodorize 3-methylindole.

The invention has the following beneficial effects:

therefore, indole and 3-methylindole are mainly harmful because of the unpleasant feeling caused by the influence on the human olfactory sensation. Aiming at the characteristic, the inventor models the olfactory receptors of indole and 3-methylindole based on the olfactory theory of two neurologists richardeale and lyndabeck in the United states, screens the perfumes according to the olfactory theory, and finds that the olfactory receptors of ester perfumes and indole and 3-methylindole have repeatability. Based on this finding, a novel method for suppressing the perception of indole and 3-methylindole by olfactory receptors by making use of an ester-based fragrance to create an olfactory illusion has been discovered. The method is simple and easy to implement, and in daily life and in scenes with indole andor 3-methylindole, the perception degree of indole andor 3-methylindole of people can be effectively reduced by spraying air fresheners, deodorants, aromatizers and the like containing ester spices. Thereby achieving the purpose of odor inhibition.

The technical solution of the present invention is further described in detail by the following examples.

Detailed Description

The present invention will be further described with reference to examples, in which various chemicals and reagents are commercially available unless otherwise specified.

Example 1

Ester fragrances were tested separately, wherein the ester fragrances were ethyl acetate, linalyl acetate, benzyl acetate, geranyl acetate, vanillyl acetate, ethyl acetoacetate, methyl dihydrojasmonate, gamma decalactone, delta decalactone, butyl butyrolactate, o-tert-butylcyclohexyl acetate.

Testing the inhibitory potential of the inhibitors produced

Human Embryonic Kidney Cells 293 (Human embryo Kidney Cells 293) are used as basic Cells, and OR2W1, OR1A1 and OR4S2 plasmids are introduced into the Human Embryonic Kidney Cells 293 by using a gene recombination technology to establish an olfactory receptor cell model. The above cells were seeded in a 96-well plate, cultured for 24 hours, then the medium was removed, washed and then medium containing 0.5mM indole CD293 was added. After 6 hours of incubation, the amount of cAMP increase in cells was quantitatively analyzed by enzyme immunoassay (ELISA) as a base value A (luminescence intensity). The same test procedure gave the base value B (luminescence intensity) of 3-methylindole.

The amount of cAMP increase after addition of ester flavor X (luminescence intensity) was measured by the same method. When X is greater than 1/2 (A orB), it is considered to have inhibitory potential and the results are shown in Table 1. The value of X is 1/2 (A orB) or more, and is represented by "+", and the value of X is less than 1/2 (A orB), and is represented by "-".

TABLE 1 inhibitory potential of various ester fragrances

Ester-type perfume	A	B
			Ethyl acetate	+	-
Linalyl acetate	+	+
			Acetic acid benzyl ester	+	+
Geraniyl acetate	+	-
			Vanillyl acetate	+	-
Acetoacetic acid ethyl ester	+	-
			Dihydrojasmonic acid methyl ester	+	-
Gamma decalactone	+	+
			Delta decalactone	+	+
Butyrylactic acid butyl ester	+	-
			Acetic acid tert-butyl cyclohexyl ester	+	+

Subjecting the obtained ester perfume to sensory test

Sensory test, 3 experimenters were selected, 3L of fresh air was filled in a polyethylene bag, an ethanol solution of indole and 3-methylindole was added to the bag, the indole concentration in the bag was 100ppm, and the 3-methylindole concentration in the bag was 25ppm, and a basic odor bag was prepared. After the volatile matter was sufficiently volatilized, three experimenters actually confirmed the smell of the air in the bag as a reference smell. Thereafter, the same standard odor bag was prepared and 200ppm of each ester fragrance was added to the bag. After volatilization, the odor is directly judged by an experimenter, wherein compared with the standard odor, the basic odor can not be completely sensed to be 6 points; a basic odor was perceived but very slightly a score of 3; the basic odor was perceived and was 0 points unchanged or worsened from baseline.

Sensory tests were conducted according to the above test standards, and the test results were 3-person average and are shown in table 2.

TABLE 2 sensory test results for different compounds

According to the results, most ester perfumes have a certain effect on inhibiting indole peculiar smell, wherein geranyl acetate, vanillyl acetate and ethyl acetoacetate have a certain effect, ethyl acetate, benzyl acetate, methyl dihydrojasmonate and butyl butyryl lactate have a good effect, and linalyl acetate, propyl decalactone, butyl decalactone and o-tert-butylcyclohexyl acetate have a best effect.

Example 2

Linalyl acetate, gamma-decalactone and o-tert-butylcyclohexyl acetate are respectively dissolved in an ethanol water solution, and the preparation concentration is 0.001 percent respectively; 0.01 percent; 0.1 percent; 1% of inhibitor.

Concentration dependency test of odor suppressing Effect

The base sachet was the sachet used in the above-described functionalization experiments. Spraying amount of 0.1mL, spraying the inhibitor into the odor bag through an injection port of the odor bag, standing at room temperature for 1 hr, and judging by direct olfaction of 3 experimenters, wherein the basic odor is 6 minutes which is completely not sensed compared with the standard odor; a basic odor was perceived but very slightly a score of 3; the basic odor was perceived and was 0 points unchanged or worsened from baseline. The average number of points obtained after the three experimenters respectively test, and the test results are shown in table 3.

TABLE 3 sensory test results for different concentrations of the compounds

	0.001％	0.01％	0.10％	0.05％	1.00％
						Linalyl acetate	1	5	6	6	6
Gamma decalactone	0	4	6	6	6
						Delta decalactone	1	5	5	6	6
Acetic acid tert-butyl cyclohexyl ester	0	5	5	6	6

In the above experiment, when the use concentration of the lipid flavor is more than 0.01%, the inhibition effect is exerted; when the concentration of the lipid flavor is more than 0.5%, the inhibition effect is good.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.

Claims (7)

1. An inhibitor of indole off-flavors, characterized in that: the perfume comprises ester perfume and solvent, wherein the mass fraction of the ester perfume is 0.01-5%;

wherein the ester perfume is one or more of ethyl acetate, linalyl acetate, benzyl acetate, geranyl acetate, vanillyl acetate, ethyl acetoacetate, methyl dihydrojasmonate, propyl decalactone, butyl butyryl butyl lactate and o-tert-butylcyclohexyl acetate.

2. The inhibitor of indole off-flavors according to claim 1, wherein: the solvent is a mixture of ethanol and water.

3. The inhibitor of indole off-taste according to claim 1, characterized in that: the mass fraction of the ester spice is 0.01-1%.

4. The inhibitor of indole off-flavors according to claim 1, wherein: the mass fraction of the ester spice is 0.5%.

5. A process for the preparation of an inhibitor of indole off-notes according to any one of claims 1 to 4, wherein: and (3) dissolving the ester spices with the mass fraction in the solvent, and fully and uniformly mixing to prepare spray.

6. The inhibitor of indole off-flavors according to claim 1, wherein: the odor inhibitor is used for removing indole odor.

7. The inhibitor of indole off-flavors according to claim 1, wherein: the odor inhibitor is used for removing odor of 3-methylindole.