CN115845154B - Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof - Google Patents
Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof Download PDFInfo
- Publication number
- CN115845154B CN115845154B CN202211499046.3A CN202211499046A CN115845154B CN 115845154 B CN115845154 B CN 115845154B CN 202211499046 A CN202211499046 A CN 202211499046A CN 115845154 B CN115845154 B CN 115845154B
- Authority
- CN
- China
- Prior art keywords
- composition
- plasma
- phosphorylcholine
- anticoagulant
- antimicrobial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 71
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 239000000463 material Substances 0.000 title claims abstract description 25
- 230000010100 anticoagulation Effects 0.000 title abstract description 16
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 30
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 30
- 238000000576 coating method Methods 0.000 claims abstract description 29
- 239000011248 coating agent Substances 0.000 claims abstract description 28
- -1 silicon quaternary ammonium salt Chemical class 0.000 claims abstract description 22
- 229950004354 phosphorylcholine Drugs 0.000 claims abstract description 17
- 150000003839 salts Chemical class 0.000 claims abstract description 15
- 229910052751 metal Inorganic materials 0.000 claims abstract description 13
- 239000002184 metal Substances 0.000 claims abstract description 13
- YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000000178 monomer Substances 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims abstract description 8
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 5
- 239000010703 silicon Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 19
- 230000000845 anti-microbial effect Effects 0.000 claims description 15
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 10
- 239000004599 antimicrobial Substances 0.000 claims description 9
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 9
- 229910052709 silver Inorganic materials 0.000 claims description 9
- 239000004332 silver Substances 0.000 claims description 9
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000001723 curing Methods 0.000 claims description 7
- 239000010936 titanium Chemical class 0.000 claims description 7
- 229910052719 titanium Inorganic materials 0.000 claims description 7
- 230000002792 vascular Effects 0.000 claims description 7
- 229910052725 zinc Chemical class 0.000 claims description 7
- 239000011701 zinc Chemical class 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- OXJGJKIURHREKH-UHFFFAOYSA-O CC(=C)C(=O)OCCP(=O)=C(O)C[N+](C)(C)C Chemical compound CC(=C)C(=O)OCCP(=O)=C(O)C[N+](C)(C)C OXJGJKIURHREKH-UHFFFAOYSA-O 0.000 claims description 6
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical class [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 6
- 150000001298 alcohols Chemical class 0.000 claims description 6
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 6
- 239000007789 gas Substances 0.000 claims description 5
- 238000001631 haemodialysis Methods 0.000 claims description 5
- 230000000322 hemodialysis Effects 0.000 claims description 5
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 5
- LWLHCZLCSDUDEL-UHFFFAOYSA-O C[N+](C)(C)CC(O)=P(=O)CCOC(=O)C=C Chemical compound C[N+](C)(C)CC(O)=P(=O)CCOC(=O)C=C LWLHCZLCSDUDEL-UHFFFAOYSA-O 0.000 claims description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 4
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical class [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical class [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- 229910052786 argon Inorganic materials 0.000 claims description 4
- 230000000747 cardiac effect Effects 0.000 claims description 4
- WSFMFXQNYPNYGG-UHFFFAOYSA-M dimethyl-octadecyl-(3-trimethoxysilylpropyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC WSFMFXQNYPNYGG-UHFFFAOYSA-M 0.000 claims description 4
- 210000003709 heart valve Anatomy 0.000 claims description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 4
- 230000002685 pulmonary effect Effects 0.000 claims description 4
- QDZRBIRIPNZRSG-UHFFFAOYSA-N titanium nitrate Chemical compound [O-][N+](=O)O[Ti](O[N+]([O-])=O)(O[N+]([O-])=O)O[N+]([O-])=O QDZRBIRIPNZRSG-UHFFFAOYSA-N 0.000 claims description 4
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 4
- ZYXMCKUKNPZFNB-UHFFFAOYSA-O C(C)P(=O)=C(O)C[N+](C)(C)C.C(C=C)(=O)N Chemical compound C(C)P(=O)=C(O)C[N+](C)(C)C.C(C=C)(=O)N ZYXMCKUKNPZFNB-UHFFFAOYSA-O 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 238000001994 activation Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 claims description 3
- 239000001307 helium Substances 0.000 claims description 3
- 229910052734 helium Inorganic materials 0.000 claims description 3
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- DCKVFVYPWDKYDN-UHFFFAOYSA-L oxygen(2-);titanium(4+);sulfate Chemical compound [O-2].[Ti+4].[O-]S([O-])(=O)=O DCKVFVYPWDKYDN-UHFFFAOYSA-L 0.000 claims description 3
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 3
- 229910000348 titanium sulfate Inorganic materials 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- NYKQZAJFUMIFKQ-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCCP(=O)(CCCCCCCCCCCCCCCCCC)OCC[N+](C)(C)C Chemical compound CCCCCCCCCCCCCCCCCCP(=O)(CCCCCCCCCCCCCCCCCC)OCC[N+](C)(C)C NYKQZAJFUMIFKQ-UHFFFAOYSA-N 0.000 claims description 2
- 239000003570 air Substances 0.000 claims description 2
- YGFACYFRHZWFHE-UHFFFAOYSA-M dimethyl-octadecyl-(3-trimethoxysilylpropyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC YGFACYFRHZWFHE-UHFFFAOYSA-M 0.000 claims description 2
- IVGAOTSELIZCDC-UHFFFAOYSA-M dodecyl-dimethyl-(3-trimethoxysilylpropyl)azanium bromide Chemical compound [Br-].CO[Si](CCC[N+](CCCCCCCCCCCC)(C)C)(OC)OC IVGAOTSELIZCDC-UHFFFAOYSA-M 0.000 claims description 2
- UBMGRWRKCJIKMQ-UHFFFAOYSA-M dodecyl-dimethyl-(3-trimethoxysilylpropyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC UBMGRWRKCJIKMQ-UHFFFAOYSA-M 0.000 claims description 2
- UVMKWFGXKGMZFM-UHFFFAOYSA-M hexadecyl-dimethyl-(3-trimethoxysilylpropyl)azanium bromide Chemical compound [Br-].CO[Si](CCC[N+](CCCCCCCCCCCCCCCC)(C)C)(OC)OC UVMKWFGXKGMZFM-UHFFFAOYSA-M 0.000 claims description 2
- FVOCUSGXQAQFAK-UHFFFAOYSA-M hexadecyl-dimethyl-(3-trimethoxysilylpropyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+](C)(C)CCC[Si](OC)(OC)OC FVOCUSGXQAQFAK-UHFFFAOYSA-M 0.000 claims description 2
- 238000000678 plasma activation Methods 0.000 claims description 2
- 238000009832 plasma treatment Methods 0.000 claims description 2
- YPNVIBVEFVRZPJ-UHFFFAOYSA-L silver sulfate Chemical compound [Ag+].[Ag+].[O-]S([O-])(=O)=O YPNVIBVEFVRZPJ-UHFFFAOYSA-L 0.000 claims description 2
- 229910000367 silver sulfate Inorganic materials 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- GBYNUONAYTWIHJ-UHFFFAOYSA-O CC(C(NCCP(=C(C[N+](C)(C)C)O)=O)=O)=C Chemical compound CC(C(NCCP(=C(C[N+](C)(C)C)O)=O)=O)=C GBYNUONAYTWIHJ-UHFFFAOYSA-O 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 210000004204 blood vessel Anatomy 0.000 claims 1
- 239000002086 nanomaterial Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 2
- 229920000642 polymer Polymers 0.000 description 11
- 230000000694 effects Effects 0.000 description 7
- 230000009471 action Effects 0.000 description 6
- 230000002429 anti-coagulating effect Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 239000002082 metal nanoparticle Substances 0.000 description 6
- 239000006185 dispersion Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 238000000879 optical micrograph Methods 0.000 description 5
- 238000002604 ultrasonography Methods 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical group [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 3
- 229920000669 heparin Polymers 0.000 description 3
- 229960002897 heparin Drugs 0.000 description 3
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- PZJJKWKADRNWSW-UHFFFAOYSA-N trimethoxysilicon Chemical group CO[Si](OC)OC PZJJKWKADRNWSW-UHFFFAOYSA-N 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 2
- 229910021607 Silver chloride Inorganic materials 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 239000004699 Ultra-high molecular weight polyethylene Substances 0.000 description 2
- SWLVFNYSXGMGBS-UHFFFAOYSA-N ammonium bromide Chemical compound [NH4+].[Br-] SWLVFNYSXGMGBS-UHFFFAOYSA-N 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 238000000635 electron micrograph Methods 0.000 description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 2
- 239000012510 hollow fiber Substances 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 239000002861 polymer material Substances 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical group [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 229920000785 ultra high molecular weight polyethylene Polymers 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- VNDYJBBGRKZCSX-UHFFFAOYSA-L zinc bromide Chemical compound Br[Zn]Br VNDYJBBGRKZCSX-UHFFFAOYSA-L 0.000 description 2
- 235000005074 zinc chloride Nutrition 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- UAYWVJHJZHQCIE-UHFFFAOYSA-L zinc iodide Chemical compound I[Zn]I UAYWVJHJZHQCIE-UHFFFAOYSA-L 0.000 description 2
- JKFYKCYQEWQPTM-UHFFFAOYSA-N 2-azaniumyl-2-(4-fluorophenyl)acetate Chemical compound OC(=O)C(N)C1=CC=C(F)C=C1 JKFYKCYQEWQPTM-UHFFFAOYSA-N 0.000 description 1
- NJNWCIAPVGRBHO-UHFFFAOYSA-N 2-hydroxyethyl-dimethyl-[(oxo-$l^{5}-phosphanylidyne)methyl]azanium Chemical group OCC[N+](C)(C)C#P=O NJNWCIAPVGRBHO-UHFFFAOYSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- LRMHMFXLXQTSBX-UHFFFAOYSA-O C(C)P(=O)=C(O)C[N+](C)(C)C.C(C(=C)C)(=O)N Chemical compound C(C)P(=O)=C(O)C[N+](C)(C)C.C(C(=C)C)(=O)N LRMHMFXLXQTSBX-UHFFFAOYSA-O 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 229910021612 Silver iodide Inorganic materials 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000002965 anti-thrombogenic effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000002840 nitric oxide donor Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- XTUSEBKMEQERQV-UHFFFAOYSA-N propan-2-ol;hydrate Chemical compound O.CC(C)O XTUSEBKMEQERQV-UHFFFAOYSA-N 0.000 description 1
- 125000001453 quaternary ammonium group Chemical group 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- ADZWSOLPGZMUMY-UHFFFAOYSA-M silver bromide Chemical compound [Ag]Br ADZWSOLPGZMUMY-UHFFFAOYSA-M 0.000 description 1
- 229940045105 silver iodide Drugs 0.000 description 1
- 229940117986 sulfobetaine Drugs 0.000 description 1
- UBZYKBZMAMTNKW-UHFFFAOYSA-J titanium tetrabromide Chemical compound Br[Ti](Br)(Br)Br UBZYKBZMAMTNKW-UHFFFAOYSA-J 0.000 description 1
- XJDNKRIXUMDJCW-UHFFFAOYSA-J titanium tetrachloride Chemical group Cl[Ti](Cl)(Cl)Cl XJDNKRIXUMDJCW-UHFFFAOYSA-J 0.000 description 1
- NLLZTRMHNHVXJJ-UHFFFAOYSA-J titanium tetraiodide Chemical compound I[Ti](I)(I)I NLLZTRMHNHVXJJ-UHFFFAOYSA-J 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 229940102001 zinc bromide Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/30—Inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
- A61L29/10—Inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
- A61L29/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/10—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
- A61L33/06—Use of macromolecular materials
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Surgery (AREA)
- Engineering & Computer Science (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Inorganic Chemistry (AREA)
- Materials Engineering (AREA)
- Materials For Medical Uses (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a composition of a nano antibacterial material and an anticoagulant component and a plasma preparation method thereof, in particular to a preparation method which comprises the steps of placing metal salt, phosphorylcholine monomer and organic silicon quaternary ammonium salt in a solvent, and uniformly dispersing and mixing; and placing the obtained mixture under an atmospheric pressure plasma region for treatment, and centrifuging to obtain supernatant, namely the prepared composition. The application of the composition of the nano inorganic antibacterial material and the anticoagulation component in preparing medical equipment with antibacterial and anticoagulation surfaces is also disclosed. The invention utilizes atmospheric pressure plasma to realize the preparation of the antibacterial anticoagulant composition at normal temperature, reduces the use of other chemicals, and simultaneously forms the composition which can form an effective coating network for the nano antibacterial material, reduces the possibility of the nano material entering the human body and improves the safety performance of the whole composition.
Description
Technical Field
The invention belongs to the field of medical materials, and particularly relates to a composition of a nano antibacterial material and an anticoagulant component and a plasma preparation method thereof.
Background
For medical devices requiring long-term contact with blood, both reduction of thrombosis and prevention of bacterial infection, it is extremely important to prepare a composition having antibacterial and anticoagulant functions. Inorganic antibacterial material is combined with classical anticoagulation component heparin as one of the most common strategies, such as Chinese patent 201410103079.0 antibacterial anticoagulation polymer material and its preparation process and application, and one kind of polymer material comprising polymer matrix material, nitric oxide donor material, hydrophilic material and antibacterial agent is disclosed. On the basis, a quaternary ammonium salt antibacterial agent can be added to improve the antibacterial performance. Japanese patent JPH10152579A ANTITHROMBOGENIC COMPOSITION TO WHICH ANTIMICROBIAL PROPERTY IS IMPARTED discloses a composition containing (a) a fat-soluble glycosaminoglycan and (B) silver and zeolite, which can easily impart antithrombotic property and antibacterial property to a polymer as a base material, useful for hemodialysis membranes, plasma separation membranes, membrane materials for artificial lungs, sheet materials for sheet lungs, and which comprises (A) (A1) an ionic complex of a glycosaminoglycan such as heparin (metal salt) and (A2) a quaternary ammonium ion such as (R1-R3 each of 1-12C alkyl, 6-12C aryl, 7-20C aralkyl; R4 is an ionic complex of 1-25C alkyl) (for example, A1: A2 in a weight ratio of 40:1:1:4) and (B) an inorganic antibacterial agent such as a silver antibacterial agent or an antibacterial glass as essential components. The composition is usually added to a substrate such as a plasma separation membrane by a mixing method, a coating method, or the like. Considering the biological activity of heparin, how to combine inorganic antibacterial materials with them to form an antibacterial anticoagulant composition is a technical problem to be solved if phosphorylcholine is used as the anticoagulant component.
Disclosure of Invention
The prior art does not relate to an antibacterial anticoagulant composition constructed by combining nano inorganic antibacterial materials with phosphorylcholine and a preparation method thereof. Aiming at the problems existing in the prior art, the invention aims to provide a composition of a nano inorganic antibacterial material and a phosphorylcholine component and a plasma preparation method thereof.
The invention uses atmospheric pressure plasma to process the mixture containing metal salt, phosphorylcholine and organosilicon quaternary ammonium salt, so that the organosilicon and phosphorylcholine form polymer, and when the monomer is polymerized, the electron in the plasma is used to reduce the metal salt into metal nano particles, and the inorganic antibacterial nano particles are wrapped in the network of the polymer, thus not only realizing the antibacterial property of the nano material, but also reducing the possibility of the nano material entering human body. Furthermore, the composition can impart anticoagulant properties and antibacterial and bacteriostatic properties as medical materials.
The invention provides a plasma preparation method of a composition of a nano inorganic antibacterial material and an anticoagulant component, which comprises the following steps:
s1) placing metal salt, phosphorylcholine monomer and organic silicon quaternary ammonium salt in a solvent, and uniformly dispersing and mixing;
s2) placing the mixture obtained in the step S1) under an atmospheric pressure plasma area for treatment, and centrifuging to obtain supernatant, namely the prepared composition.
Further, the metal salt is selected from the group consisting of an inorganic salt of silver, an inorganic salt of titanium, and an inorganic salt of zinc.
Further, the inorganic salt of silver is selected from silver halide, silver nitrate and silver sulfate; the inorganic salt of titanium is selected from titanium halide, titanium sulfate and titanium nitrate; the inorganic salt of zinc is selected from zinc halide, zinc nitrate and zinc sulfate. Still further, the silver halide is selected from silver chloride, silver bromide, or silver iodide; the titanium halide is selected from titanium chloride, titanium bromide or titanium iodide; the zinc halide is selected from zinc chloride, zinc bromide or zinc iodide.
Further, the phosphorylcholine monomer is at least one selected from methacryloxyethyl phosphorylcholine, methacryloxyethyl sulfobetaine, 2-methacryloxyethyl phosphorylcholine, acryloxyethyl phosphorylcholine, acrylamide ethyl phosphorylcholine, methacrylamide ethyl phosphorylcholine, dipalmitoyl phosphorylcholine, dimyristoyl phosphorylcholine or distearyl phosphorylcholine.
Further, the organic silicon quaternary ammonium salt is at least one selected from the group consisting of dimethyl dodecyl [3- (trimethoxysilyl) propyl ] ammonium chloride, dimethyl dodecyl [3- (trimethoxysilyl) propyl ] ammonium bromide, dimethyl octadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride, dimethyl octadecyl [3- (trimethoxysilyl) propyl ] ammonium bromide, dimethyl hexadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride and dimethyl hexadecyl [3- (trimethoxysilyl) propyl ] ammonium bromide.
Further, the solvent is an aqueous solution containing alcohols;
further, the alcohols include ethanol, isopropanol, and ethylene glycol.
Further, the mass fraction of the alcohols is 10% -100%;
further, the weight ratio of the metal salt to the phosphorylcholine monomer to the organosilicon quaternary ammonium salt is 0.1-0.5: 0.5 to 1.5:1 to 4.
Further, the atmospheric pressure plasma is obtained by a plasma generating device selected from the group consisting of a dielectric barrier discharge device, a plasma jet device, a creeping discharge device.
Further, the plasma treatment time is 10 to 60 minutes.
Further, the plasma processing gas is selected from one or more sources of air, argon, helium, nitrogen.
Further, the speed of centrifugation is 200rpm to 2000rpm.
In another aspect, the invention provides a composition of a nano inorganic antibacterial material and an anticoagulant component, wherein the composition is obtained by the plasma preparation method.
In yet another aspect, the invention provides a method of making an antimicrobial anticoagulant coating comprising immersing a substrate in the composition to form a coating on the surface of the substrate.
Further, the preparation method for forming the antibacterial anticoagulant coating further comprises a step of further curing.
Further, the curing method is to cure the substrate soaked by the composition at 20-80 ℃ for 1-24 hours.
In yet another aspect, the invention provides a method of preparing an antimicrobial anticoagulant coating comprising spraying the composition onto the surface of an activated substrate to form a coating.
Further, the preparation method for forming the antibacterial anticoagulant coating further comprises a step of further curing.
Further, the curing method is to cure the substrate soaked by the composition at 20-80 ℃ for 1-24 hours.
Further, the substrate is selected from a polymeric material or a metallic material.
Further, the activation is to treat the surface of the substrate with one of corona activation and plasma activation.
In a further aspect the invention provides an antimicrobial anticoagulant coating obtainable by the above-described preparation method.
In yet another aspect, the present invention provides a medical device selected from the group consisting of hemodialysis medical devices, extracorporeal circulation medical devices, prosthetic heart valves, cardiac pacemakers, vascular prostheses, vascular stents, epicardial pulmonary oxygenation devices, surgical wires and catheters, having the above-described antimicrobial anticoagulant coating.
In a further aspect, the invention provides the use of a composition comprising a nano-inorganic antimicrobial material as described above and an anticoagulant component in the preparation of a medical device having an antimicrobial and anticoagulant surface.
Further, the medical device is selected from the group consisting of hemodialysis medical devices, extracorporeal circulation medical devices, prosthetic heart valves, cardiac pacemakers, vascular prostheses, vascular stents, epicardial pulmonary oxygenation devices, surgical wires, and catheter devices.
Compared with the prior art, the preparation of the antibacterial and anticoagulant composition is realized at normal temperature by utilizing the atmospheric pressure plasma, the use of other chemicals is reduced, meanwhile, the organosilicon can form an effective network for the nano antibacterial material, the possibility that the nano material enters a human body is reduced, and the overall safety performance of the composition is improved.
The preparation method of the composition is simple and has high preparation speed. Compared with the mixing of inorganic nano materials and polymers, the mixing of metal salt, phosphorylcholine and organosilicon quaternary ammonium salt in a solvent is easier to realize uniformity of the three, and under the action of plasma, the metal salt is reduced into the metal material which is easier to be distributed in the network structure of the polymers.
The composition obtained by the invention can be easily adhered to the surface of a substrate, and can provide antibacterial and anticoagulant effects for the surface of the substrate. And the composition is easy to store, providing a quick and efficient method for obtaining an anticoagulant and antibacterial surface.
Drawings
FIG. 1 is an optical micrograph of a mixture of a metal salt, phosphorylcholine, a quaternary organosilicon salt in a solvent;
FIG. 2 is an optical micrograph of the antibacterial anticoagulant composition prepared in example 1;
FIG. 3 is an electron micrograph of platelet adhesion of the untreated material surface;
FIG. 4 is an electron micrograph of platelet adhesion of the composition prepared in example 1;
FIG. 5 is a graph showing the antibacterial effect of the composition prepared in example 1 according to the zone of inhibition test;
FIG. 6 is an optical micrograph of the antibacterial anticoagulant composition prepared in example 2.
Detailed Description
The following detailed description of the present invention will be made in detail to make the above objects, features and advantages of the present invention more apparent, but should not be construed to limit the scope of the present invention.
In the technical scheme of the invention, the discharge power of the plasma is not limited, and because the discharge powers generated by different discharge devices are different, the discharge power can be adjusted according to the conditions of the generation device, the gas and the flow and the sample to be processed by a person skilled in the art, and only the power needs to be reduced as much as possible while the effect is generated, so that the energy sources and the energy consumption are saved. Meanwhile, on the basis of the present invention, conditions such as a plasma generation mode, a gas pressure generation, etc. may be adjusted, and oxygen-containing functional groups may be added to the discharge gas.
Example 1
Silver nitrate, 2-methacryloxyethyl phosphorylcholine and dimethyl dodecyl [3- (trimethoxy silicon group) propyl ] ammonium chloride are mixed according to the weight ratio of 0.1:0.5:1, and 10% ethanol water solution, and performing dispersion mixing under the action of ultrasound, and observing with an optical microscope to find that the three components are uniformly mixed, and the result is shown in figure 1. And (3) placing the uniform mixture under an atmospheric pressure argon plasma jet region for treatment for 15min, and centrifuging at a speed of 500rpm to obtain a supernatant, namely the prepared composition. Fig. 2 is an optical micrograph of the prepared composition showing the polymer network structure and the metal nanoparticles formed.
The anticoagulation effect of the composition was analyzed according to the platelet adhesion test, using an untreated polyvinyl chloride substrate as a control, using a vinyl chloride substrate impregnated with the composition prepared as described above and heat-cured as a test sample, immersing both samples in platelet-containing plasma, incubating at 37℃for 3 hours, fixing with glutaraldehyde, dehydrating with an ethanol/water gradient, and observing the adhesion of platelets to the surface of a tube using a scanning electron microscope, and the results are shown in FIGS. 3 and 4, respectively. It can be seen that a large amount of platelets adhere to the surface of the untreated material, while the sample surface obtained by the method has little platelet adhesion, which indicates that the technical scheme can prepare the composition with anticoagulation effect.
The antibacterial and bacteriostatic effects of the compositions of the present invention were tested using the classical zone of inhibition method, the results of which are shown in figure 5. It can be seen that the composition obtained by the preparation method can obviously inhibit the growth of staphylococcus aureus, and forms a bacteriostasis zone. Therefore, the technical scheme can prepare the composition with antibacterial effect.
Example 2
Zinc chloride, 2-methacryloxyethyl phosphorylcholine and dimethyl octadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride are mixed according to the weight ratio of 0.2:1:2, and in 30% ethanol water solution, and performing dispersion mixing under the action of ultrasound, and observing with an optical microscope to find that the three components are uniformly mixed. And (3) placing the uniform mixture under an atmospheric air dielectric barrier discharge plasma region for treatment for 20min, and centrifuging at a speed of 300rpm to obtain supernatant, namely the prepared composition. Fig. 6 is an optical micrograph of the resulting composition showing the polymer network structure and the metal nanoparticles formed.
The anticoagulation effect of the coating was analyzed according to the platelet adhesion test, and anticoagulation comparison was performed using an untreated stainless steel plate as a control sample and an activated stainless steel plate impregnated with the above composition as a control sample. The results show that the composition obtained by the invention has an anticoagulant effect. Meanwhile, the antibacterial effect of the coating is tested by using a classical inhibition zone method, and the result shows that the coating prepared by the preparation method can obviously inhibit the growth of escherichia coli.
Example 3
Titanium sulfate, acryloyloxyethyl phosphorylcholine and dimethyl hexadecyl [3- (trimethoxy silicon base) propyl ] ammonium bromide are mixed according to the weight ratio of 0.2:0.6:1.2, and in 40% glycol aqueous solution, and carrying out dispersion mixing under the action of ultrasound, and observing through an optical microscope, the three components are found to be uniformly mixed. And (3) placing the uniform mixture under an atmospheric pressure nitrogen surface discharge plasma area for 30min, and centrifuging at a speed of 1000rpm to obtain a supernatant, namely the prepared composition. The polymer network structure formed and the metal nanoparticles can be seen. The anticoagulation effect of the coating was analyzed according to the platelet adhesion test, and anticoagulation comparison was performed using an untreated hollow fiber membrane as a control sample and an activated hollow fiber membrane impregnated with the above composition as a control sample. The results show that the composition obtained by the invention has an anticoagulant effect. Meanwhile, the antibacterial effect of the coating is tested by using a classical inhibition zone method, and the result shows that the coating prepared by the preparation method can obviously inhibit the growth of escherichia coli.
Example 4
Silver chloride, acrylamide ethyl phosphorylcholine and dimethyl octadecyl [3- (trimethoxy silicon base) propyl ] ammonium bromide are mixed according to the weight ratio of 0.3:0.8:2, and 50% glycol water solution, and carrying out dispersion mixing under the action of ultrasound, and observing through an optical microscope, and finding that the three components are uniformly mixed. And (3) placing the uniform mixture under an atmospheric argon surface discharge plasma region for 25min, and centrifuging at 800rpm to obtain supernatant, namely the prepared composition. The polymer network structure formed and the metal nanoparticles can be seen. The anticoagulation effect of the coating was analyzed according to the platelet adhesion test, with untreated polyurethane catheters as control samples and with activated polyurethane catheters impregnated with the above composition as control samples, for anticoagulation comparison. The results show that the composition obtained by the invention has an anticoagulant effect. Meanwhile, the antibacterial effect of the coating is tested by using a classical inhibition zone method, and the result shows that the coating prepared by the preparation method can obviously inhibit the growth of escherichia coli.
Example 5
Zinc sulfate, acryloyloxyethyl phosphorylcholine and dimethyl octadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride are mixed according to the weight ratio of 0.5:0.8:3, and in 40% isopropanol water solution, and carrying out dispersion mixing under the action of ultrasound, and observing through an optical microscope, the three components are uniformly mixed. And (3) placing the uniform mixture under an atmospheric helium surface discharge plasma region for 40min, and centrifuging at a speed of 1500rpm to obtain a supernatant, namely the prepared composition. The polymer network structure formed and the metal nanoparticles can be seen. The anticoagulation effect of the coating was analyzed according to the platelet adhesion test, with untreated ultra-high molecular weight polyethylene suture as a control, and with activated ultra-high molecular weight polyethylene suture impregnated with the above composition as a control, anticoagulation comparison was performed. The results show that the composition obtained by the invention has an anticoagulant effect. Meanwhile, the antibacterial effect of the coating is tested by using a classical inhibition zone method, and the result shows that the coating prepared by the preparation method can obviously inhibit the growth of staphylococcus aureus.
Claims (20)
1. A method for preparing plasma of a composition of a nano inorganic antibacterial material and an anticoagulant component, which is characterized by comprising the following steps:
s1) placing metal salt, phosphorylcholine monomer and organic silicon quaternary ammonium salt in a solvent, and uniformly dispersing and mixing;
s2) placing the mixture obtained in the step S1) under an atmospheric pressure plasma area for treatment, and centrifuging to obtain supernatant, namely the prepared composition;
the metal salt is selected from inorganic salts of silver, titanium or zinc; the inorganic salt of silver is selected from silver halide, silver nitrate and silver sulfate; the inorganic salt of titanium is selected from titanium halide, titanium sulfate and titanium nitrate; the inorganic salt of zinc is selected from zinc halide, zinc nitrate and zinc sulfate.
2. The method for preparing the plasma according to claim 1, wherein the weight ratio of the metal salt, phosphorylcholine monomer and organosilicon quaternary ammonium salt is (0.1-0.5): 0.5 to 1.5:1 to 4.
3. The method of claim 1, wherein the phosphorylcholine monomer is at least one selected from the group consisting of methacryloxyethyl phosphorylcholine, 2-methacryloxyethyl phosphorylcholine, acryloxyethyl phosphorylcholine, acrylamide ethyl phosphorylcholine, methacrylamidoethyl phosphorylcholine, dipalmitoyl phosphorylcholine, dimyristoyl phosphorylcholine, and distearyl phosphorylcholine.
4. The method for preparing plasma according to claim 1, wherein the organic silicon quaternary ammonium salt is at least one selected from the group consisting of dimethyldodecyl [3- (trimethoxysilyl) propyl ] ammonium chloride, dimethyldodecyl [3- (trimethoxysilyl) propyl ] ammonium bromide, dimethyloctadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride, dimethyloctadecyl [3- (trimethoxysilyl) propyl ] ammonium bromide, dimethylhexadecyl [3- (trimethoxysilyl) propyl ] ammonium chloride, and dimethylhexadecyl [3- (trimethoxysilyl) propyl ] ammonium bromide.
5. The method of claim 1, wherein the solvent is an aqueous solution containing alcohols.
6. The method of claim 5, wherein the alcohols include ethanol, isopropanol, and ethylene glycol.
7. The method of preparing plasma according to claim 5, wherein the mass fraction of the alcohols is 10% to 100%.
8. The method of claim 1, wherein the atmospheric pressure plasma is obtained by a plasma generating device selected from the group consisting of a dielectric barrier discharge device, a plasma jet device, and a creeping discharge device.
9. The method of claim 8, wherein the plasma treatment time is 10 to 60 minutes.
10. The method of claim 8, wherein the plasma processing gas is selected from the group consisting of one or more sources of air, argon, helium, and nitrogen.
11. A combination of a nano-inorganic antimicrobial material and an anticoagulant component, characterized in that said combination is obtained by a plasma preparation method according to any one of claims 1-10.
12. A method of producing an antimicrobial anticoagulant coating comprising spraying the composition of claim 11 onto the surface of an activated substrate to form a coating; or immersing the substrate in the composition of claim 11 to form a coating on the surface of the substrate.
13. The method of claim 12, wherein the method of forming an antimicrobial anticoagulant coating further comprises the step of further curing.
14. The method of claim 13, wherein the curing is performed by curing the substrate soaked in the composition at 20 to 80 ℃ for 1 to 24 hours.
15. The method of claim 12, wherein the substrate is selected from the group consisting of polymeric and metallic substrates.
16. The method of claim 12, wherein the activating is treating the surface of the substrate with one of corona activation and plasma activation.
17. An antimicrobial anticoagulant coating, characterized in that it is obtained by the preparation method according to any one of claims 12 to 16.
18. A medical device of an antimicrobial anticoagulant coating, wherein the antimicrobial anticoagulant coating is according to claim 17, the medical device being selected from the group consisting of hemodialysis medical devices, extracorporeal circulation medical devices, prosthetic heart valves, cardiac pacemakers, vascular prostheses, vascular stents, epicardial pulmonary oxygenation devices, surgical wires and catheters.
19. Use of a combination of a nano-inorganic antimicrobial material according to claim 11 and an anticoagulant component for the preparation of a medical device having an antimicrobial and anticoagulant surface.
20. The use according to claim 19, said medical device being selected from the group consisting of hemodialysis medical devices, extracorporeal circulation medical devices, prosthetic heart valves, cardiac pacemakers, prosthetic blood vessels, vascular stents, epicardial pulmonary oxygenation devices, surgical wires and catheter devices.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211499046.3A CN115845154B (en) | 2022-11-28 | 2022-11-28 | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof |
| PCT/CN2023/133429 WO2024114479A1 (en) | 2022-11-28 | 2023-11-22 | Nano antibacterial material and anticoagulant component composition and plasma preparation method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211499046.3A CN115845154B (en) | 2022-11-28 | 2022-11-28 | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115845154A CN115845154A (en) | 2023-03-28 |
| CN115845154B true CN115845154B (en) | 2024-03-19 |
Family
ID=85667036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211499046.3A Active CN115845154B (en) | 2022-11-28 | 2022-11-28 | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN115845154B (en) |
| WO (1) | WO2024114479A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115845154B (en) * | 2022-11-28 | 2024-03-19 | 中国科学院深圳先进技术研究院 | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10114660A (en) * | 1996-10-08 | 1998-05-06 | Toyobo Co Ltd | Antithrombogenic composition having antimicrobial property |
| WO2008144247A1 (en) * | 2007-05-14 | 2008-11-27 | Tyco Healthcare Group Lp | Antimicrobial materials and coatings |
| KR20120061241A (en) * | 2010-12-03 | 2012-06-13 | (주)엔피텍 | Polyester Master Batch with High Antibiotic Property and Method for Preparing the Same |
| CN103008684A (en) * | 2013-01-21 | 2013-04-03 | 北京大学 | Method for preparing metal nanoparticles by means of atmospheric pressure cold plasmas |
| CN109517121A (en) * | 2018-11-01 | 2019-03-26 | 陕西科技大学 | A kind of organosilicon quaternary ammonium salt/(Ag/ZnO) nano antibacterial agent and its preparation method and application |
| CN110366454A (en) * | 2017-01-31 | 2019-10-22 | 弗劳恩霍夫应用研究促进协会 | For manufacturing the atmospheric pressure plasma method of plasma polymer coating |
| CN110665374A (en) * | 2019-09-19 | 2020-01-10 | 深圳安吉尔饮水产业集团有限公司 | Preparation method of quaternary ammonium salt antibacterial material and antibacterial material prepared by preparation method |
| CN110665071A (en) * | 2019-10-14 | 2020-01-10 | 中国科学院长春应用化学研究所 | Antibacterial and anticoagulant type coating, functional material with antibacterial and anticoagulant type coating and preparation method of functional material |
| CN111298202A (en) * | 2020-03-04 | 2020-06-19 | 四川大学 | Long-acting anticoagulant antibacterial coating for extracorporeal membrane oxygenation device (ECMO) and preparation method thereof |
| CN111424432A (en) * | 2020-03-05 | 2020-07-17 | 华南理工大学 | Non-dissolution type long-acting antibacterial protective non-woven fabric and preparation method and application thereof |
| CN113040135A (en) * | 2021-02-05 | 2021-06-29 | 李海亮 | Nano-silver composite hydrosol disinfectant and preparation method thereof |
| CN113667173A (en) * | 2021-08-20 | 2021-11-19 | 宁波健世科技股份有限公司 | Method for manufacturing high polymer valve leaflet with phosphorylcholine anticoagulation surface |
| CN113813450A (en) * | 2021-09-18 | 2021-12-21 | 康健仿生(清远)科技有限公司 | Medical catheter surface modification method considering hydrophilicity, low friction and bacterial adhesion resistance and application |
| CN115198525A (en) * | 2022-09-02 | 2022-10-18 | 绍兴阿米科新材料科技有限公司 | Method for preparing betaine and nano metal particle dual antibacterial cotton fabric based on plasma technology and application |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10152579A (en) * | 1996-11-25 | 1998-06-09 | Toyobo Co Ltd | Antithrombogenic composition to which antimicrobial property is imparted |
| CA3117019A1 (en) * | 2013-04-26 | 2014-10-30 | Biointeractions Limited | Bioactive coatings |
| CN107775014B (en) * | 2017-09-06 | 2020-05-22 | 深圳先进技术研究院 | Method for preparing noble metal/graphene composite nano material by using atmospheric pressure cold plasma |
| JP7158993B2 (en) * | 2018-10-15 | 2022-10-24 | 千秋 東 | Phosphorylcholine group-containing copolymer and biomedical substrate |
| KR20220111798A (en) * | 2021-02-02 | 2022-08-10 | 주식회사 인성메디칼 | Method for preparing medical device formed antibacterial and antithrombotic coating layer |
| CN115845154B (en) * | 2022-11-28 | 2024-03-19 | 中国科学院深圳先进技术研究院 | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof |
-
2022
- 2022-11-28 CN CN202211499046.3A patent/CN115845154B/en active Active
-
2023
- 2023-11-22 WO PCT/CN2023/133429 patent/WO2024114479A1/en unknown
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10114660A (en) * | 1996-10-08 | 1998-05-06 | Toyobo Co Ltd | Antithrombogenic composition having antimicrobial property |
| WO2008144247A1 (en) * | 2007-05-14 | 2008-11-27 | Tyco Healthcare Group Lp | Antimicrobial materials and coatings |
| KR20120061241A (en) * | 2010-12-03 | 2012-06-13 | (주)엔피텍 | Polyester Master Batch with High Antibiotic Property and Method for Preparing the Same |
| CN103008684A (en) * | 2013-01-21 | 2013-04-03 | 北京大学 | Method for preparing metal nanoparticles by means of atmospheric pressure cold plasmas |
| CN110366454A (en) * | 2017-01-31 | 2019-10-22 | 弗劳恩霍夫应用研究促进协会 | For manufacturing the atmospheric pressure plasma method of plasma polymer coating |
| CN109517121A (en) * | 2018-11-01 | 2019-03-26 | 陕西科技大学 | A kind of organosilicon quaternary ammonium salt/(Ag/ZnO) nano antibacterial agent and its preparation method and application |
| CN110665374A (en) * | 2019-09-19 | 2020-01-10 | 深圳安吉尔饮水产业集团有限公司 | Preparation method of quaternary ammonium salt antibacterial material and antibacterial material prepared by preparation method |
| CN110665071A (en) * | 2019-10-14 | 2020-01-10 | 中国科学院长春应用化学研究所 | Antibacterial and anticoagulant type coating, functional material with antibacterial and anticoagulant type coating and preparation method of functional material |
| CN111298202A (en) * | 2020-03-04 | 2020-06-19 | 四川大学 | Long-acting anticoagulant antibacterial coating for extracorporeal membrane oxygenation device (ECMO) and preparation method thereof |
| CN111424432A (en) * | 2020-03-05 | 2020-07-17 | 华南理工大学 | Non-dissolution type long-acting antibacterial protective non-woven fabric and preparation method and application thereof |
| CN113040135A (en) * | 2021-02-05 | 2021-06-29 | 李海亮 | Nano-silver composite hydrosol disinfectant and preparation method thereof |
| CN113667173A (en) * | 2021-08-20 | 2021-11-19 | 宁波健世科技股份有限公司 | Method for manufacturing high polymer valve leaflet with phosphorylcholine anticoagulation surface |
| CN113813450A (en) * | 2021-09-18 | 2021-12-21 | 康健仿生(清远)科技有限公司 | Medical catheter surface modification method considering hydrophilicity, low friction and bacterial adhesion resistance and application |
| CN115198525A (en) * | 2022-09-02 | 2022-10-18 | 绍兴阿米科新材料科技有限公司 | Method for preparing betaine and nano metal particle dual antibacterial cotton fabric based on plasma technology and application |
Non-Patent Citations (3)
| Title |
|---|
| Highly hydrophilic and antibiofouling surface of zwitterionic polymer immobilized on polydimethylsiloxane by initiator-free atmospheric plasma-induced polymerization;Chen Jung-San;SURFACE AND COATING TECHNOLOGY;第344卷;621-625 * |
| 基于气液等离子体的柔性材料表界面设计与功能化构筑;高明;中国博士学位论文全文数据库;B016-186 * |
| 等离子体引发聚合制备季铵盐类阳离子型电解质;何彦刚;中国优秀硕士学位论文全文数据库;B016-51 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115845154A (en) | 2023-03-28 |
| WO2024114479A1 (en) | 2024-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3050920B2 (en) | Irradiation polymerization method using plasma and γ-ray together for surface modification | |
| DE60214513T2 (en) | PROCESS FOR SURFACE MODIFICATION | |
| US5378146A (en) | Polyurethane biomedical devices & method of making same | |
| CA2052793C (en) | Combined plasma and gamma radiation polymerization method for modifying surfaces | |
| DE60201889T2 (en) | METHOD FOR PRODUCING ANTIMICROBIAL POLYMER SURFACES | |
| US4613517A (en) | Heparinization of plasma treated surfaces | |
| US3453194A (en) | Anticoagulant surfaces produced by radiation grafting heparin to a silicone substrate | |
| CN115845154B (en) | Composition of nano antibacterial material and anticoagulation component and plasma preparation method thereof | |
| US5094876A (en) | Surface modified surgical instruments, devices, implants, contact lenses and the like | |
| CN112717207A (en) | Long-acting antibacterial multifunctional coating based on bionic dopamine and preparation method and application thereof | |
| DE2326863A1 (en) | PROCESS FOR MANUFACTURING BIOCOMPATIBLE AND BIOFUNCTIONAL MATERIALS | |
| JP2001500408A (en) | EPTFE small diameter vascular grafts with significant patency enhancement via a surface coating containing covalent heparin | |
| EP1028819A1 (en) | Method for modifying the surface of an object | |
| EP1220694A2 (en) | Nitric oxide-modified linear poly (ethylenimine) fibers | |
| WO1989004682A1 (en) | Antimicrobial device and method | |
| WO1989009246A1 (en) | Low coefficient of friction surface | |
| PT2012839E (en) | Novel antimicrobial substrates and uses thereof | |
| CN113713183A (en) | Biomedical coating with excellent long-acting anticoagulation, antibiosis and anti-fouling performances and preparation method thereof | |
| JP4273965B2 (en) | Antithrombotic composition and medical device having the same | |
| Duta et al. | Surface modification of poly (vinylchloride) for manufacturing advanced catheters | |
| CN110772662A (en) | Antibacterial expanded polytetrafluoroethylene facial implant material and preparation process thereof | |
| CN1748804A (en) | Anti-blood coagulation surface modifying method of artificial implant | |
| Verma et al. | Antimicrobial finishing of polypropylene fabric using bioactive nanogels | |
| CN104672483B (en) | A kind of preparation of polymer liquid crystal membrane material of imitative membrane structure and application thereof | |
| CN116265034A (en) | Multifunctional anticoagulation coating and plasma preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |