CN115843797A - New application of 2-methylamino ethanol, drug for preventing and treating root-knot nematodes and prevention and treatment method - Google Patents
New application of 2-methylamino ethanol, drug for preventing and treating root-knot nematodes and prevention and treatment method Download PDFInfo
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- CN115843797A CN115843797A CN202211596946.XA CN202211596946A CN115843797A CN 115843797 A CN115843797 A CN 115843797A CN 202211596946 A CN202211596946 A CN 202211596946A CN 115843797 A CN115843797 A CN 115843797A
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- OPKOKAMJFNKNAS-UHFFFAOYSA-N N-methylethanolamine Chemical compound CNCCO OPKOKAMJFNKNAS-UHFFFAOYSA-N 0.000 title claims abstract description 90
- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 30
- 239000003814 drug Substances 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims description 14
- 229940079593 drug Drugs 0.000 title abstract description 7
- 230000002265 prevention Effects 0.000 title description 5
- 239000005660 Abamectin Substances 0.000 claims abstract description 5
- UOORRWUZONOOLO-OWOJBTEDSA-N (E)-1,3-dichloropropene Chemical compound ClC\C=C\Cl UOORRWUZONOOLO-OWOJBTEDSA-N 0.000 claims abstract description 3
- 239000005644 Dazomet Substances 0.000 claims abstract description 3
- 239000005959 Fosthiazate Substances 0.000 claims abstract description 3
- 239000002169 Metam Substances 0.000 claims abstract description 3
- LFHISGNCFUNFFM-UHFFFAOYSA-N chloropicrin Chemical compound [O-][N+](=O)C(Cl)(Cl)Cl LFHISGNCFUNFFM-UHFFFAOYSA-N 0.000 claims abstract description 3
- QAYICIQNSGETAS-UHFFFAOYSA-N dazomet Chemical compound CN1CSC(=S)N(C)C1 QAYICIQNSGETAS-UHFFFAOYSA-N 0.000 claims abstract description 3
- DUFVKSUJRWYZQP-UHFFFAOYSA-N fosthiazate Chemical compound CCC(C)SP(=O)(OCC)N1CCSC1=O DUFVKSUJRWYZQP-UHFFFAOYSA-N 0.000 claims abstract description 3
- HYVVJDQGXFXBRZ-UHFFFAOYSA-N metam Chemical compound CNC(S)=S HYVVJDQGXFXBRZ-UHFFFAOYSA-N 0.000 claims abstract description 3
- VYHVQEYOFIYNJP-UHFFFAOYSA-N methyl thiocyanate Chemical compound CSC#N VYHVQEYOFIYNJP-UHFFFAOYSA-N 0.000 claims abstract description 3
- UOORRWUZONOOLO-UHFFFAOYSA-N telone II Natural products ClCC=CCl UOORRWUZONOOLO-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- RRZXIRBKKLTSOM-XPNPUAGNSA-N avermectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 RRZXIRBKKLTSOM-XPNPUAGNSA-N 0.000 claims 1
- 238000009313 farming Methods 0.000 claims 1
- 241000244206 Nematoda Species 0.000 abstract description 22
- 230000002147 killing effect Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 8
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 abstract description 4
- 229950008167 abamectin Drugs 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 4
- 239000005645 nematicide Substances 0.000 abstract description 4
- 244000144972 livestock Species 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 206010059866 Drug resistance Diseases 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract description 2
- 241000282414 Homo sapiens Species 0.000 abstract description 2
- 230000000443 biocontrol Effects 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 241000243786 Meloidogyne incognita Species 0.000 description 14
- 240000008067 Cucumis sativus Species 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 241001143352 Meloidogyne Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000003958 fumigation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 239000005961 Ethoprophos Substances 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 235000001231 Streptopus amplexifolius Nutrition 0.000 description 1
- 244000305550 Streptopus amplexifolius Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- QGLZXHRNAYXIBU-WEVVVXLNSA-N aldicarb Chemical compound CNC(=O)O\N=C\C(C)(C)SC QGLZXHRNAYXIBU-WEVVVXLNSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001728 capsicum frutescens Substances 0.000 description 1
- DUEPRVBVGDRKAG-UHFFFAOYSA-N carbofuran Chemical compound CNC(=O)OC1=CC=CC2=C1OC(C)(C)C2 DUEPRVBVGDRKAG-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- VJYFKVYYMZPMAB-UHFFFAOYSA-N ethoprophos Chemical compound CCCSP(=O)(OCC)SCCC VJYFKVYYMZPMAB-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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Abstract
The invention relates to a novel drug for preventing and treating root-knot nematodes, which specifically comprises the following components: 2-methylaminoethanol. When the compound preparation is used for preventing and treating root-knot nematodes, the 2-methylamino ethanol can be used alone, or can be used together with one or more of fosthiazate, abamectin, 1, 3-dichloropropene, trichloronitromethane, methyl thiocyanate, dazomet, metam or the like or be used together with biocontrol bacteria and fungi. Increase the effect of killing nematodes and avoid the generation of drug resistance of nematodes after a single medicament is used for a long time. The 2-methylamino ethanol is used as a material for preventing and controlling the nematodes, is an excellent nematicide substitute, has good nematicide effect, is low in toxicity, and is friendly to the environment, human beings and livestock.
Description
Technical Field
The invention belongs to the technical field of crop pest control, and particularly relates to a drug for preventing and treating root-knot nematodes by using 2-methylaminoethanol and a prevention and treatment method.
Background
Root-knot nematodes are one of the plant parasitic nematodes which threaten agricultural production most seriously in current production, are distributed in many countries and regions around the world and can infect 5500 various plants, including food crops, vegetables, fruit trees, landscape plants and the like. In recent years, with the development of facility agriculture in China, the planting area of greenhouse vegetables is rapidly increased, the generation area of root-knot nematodes is continuously enlarged, the yield of the vegetables can be reduced by about 70% when the root-knot nematodes are seriously generated, and very serious economic loss is caused.
Chemical control in the prior production is an important measure for preventing and treating root-knot nematodes, some nematicides which are widely used in production, such as aldicarb, methyl isophos, ethoprophos, carbofuran and the like, are highly toxic, so that the quality of vegetables is seriously reduced in the using process, certain threats are caused to the health of people and livestock, soil and water resources are polluted, and ecological balance is damaged, so that the hot point of attention of people is paid to how to reduce the use of chemical pesticides or develop safe and efficient substitutes.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a novel drug for preventing and treating root-knot nematodes and a method for preventing and treating the root-knot nematodes. Specifically, the method comprises the following steps:
a medicament for the control of root-knot nematodes comprising 2-methylaminoethanol.
When the compound preparation is used for preventing and treating root-knot nematodes, the 2-methylamino ethanol can be used alone, or can be used together with one or more of fosthiazate, abamectin, 1, 3-dichloropropene, trichloronitromethane, methyl thiocyanate, dazomet, metam or the like or be used together with biocontrol bacteria and fungi. Increase the effect of killing nematodes and avoid the generation of drug resistance of nematodes after a single medicament is used for a long time.
Specifically, the concentration of the 2-methylamino ethanol is 0.14-3.75mM when the root-knot nematode is controlled.
A method for preventing and treating root-knot nematode uses the above-mentioned medicines.
Specifically, when the root-knot nematode is controlled in the slack season, 2-methylaminoethanol is applied to the surface layer of soil, and a high-temperature greenhouse is combined for controlling the root-knot nematode.
When the root-knot nematode is controlled in the crop cultivation period, the 2-methylamino ethanol is applied to the soil 15-30cm away to control the root-knot nematode.
2-methylaminoethanol (2- (methylamino) -Ethanol, CAS: 109-83-1), structural formula:
the 2-methylamino ethanol is widely used as a medicine raw material, is an intermediate for manufacturing dyes, fiber treating agents, food nutrition additives and the like, and has the advantages of rich material sources and simple synthesis steps. In addition, the 2-methylamino ethanol has solubilization effect on the raw medicines and is helpful for emulsification. Therefore, the 2-methylamino ethanol serving as a material for preventing and controlling the nematodes is an excellent nematicide substitute, has a good nematode killing effect, is low in toxicity, and is friendly to the environment, human and livestock. />
Drawings
The advantages and benefits of the present invention will become apparent to those of ordinary skill in the art upon reading the following detailed description of the specific embodiments. The drawings are illustrative and not to be considered as limiting the invention. In the drawings:
FIG. 1 shows the fumigating effect of 2-methylaminoethanol with different concentration gradients on Meloidogyne incognita;
FIG. 2 is a microscopic photograph of nematodes after methylaminoethanol fumigation;
FIG. 3 shows the direct contact killing effect of 2-methylaminoethanol with different concentration gradients on Meloidogyne incognita.
Detailed Description
To facilitate an understanding of the present application, the present application is described more fully below.
The 2-methylaminoethanol (CAS: 109-83-1) used in the following examples was obtained from sigma.
The root-knot nematodes used were: and (3) second-instar larvae of Meloidogyne incognita.
Mature nematode egg masses are picked from capsicum frutescens roots used for culturing meloidogyne incognita in laboratories, sterilized for 1min by 0.5% sodium hypochlorite, and washed clean by sterile water. Collecting the egg mass into a sterilized culture dish, adding a small amount of sterile water, incubating at a constant temperature of 28 ℃, and collecting the incubated nematodes for later use after 48 hours.
Determination of fumigating and killing effect of 2-methylamino ethanol with different concentrations on meloidogyne incognita
And (3) adopting a sterile two-grid culture dish to carry out fumigation and killing effect determination on the meloidogyne incognita by using 2-methylamino ethanol.
In the first of the two dishes, 1ml of 2-methylaminoethanol diluted with sterile water was added, and in the second, 1ml of nematode suspension containing 100 larvae of the second instar was added. The petri dish was sealed with a sealing film and placed in an incubator at 26 ℃ for 12h. Each treatment was performed on 5 dishes and repeated 3 times.
The concentration of 2-methylaminoethanol was 0.75mM, 1mM, 1.5mM, 1.8mM, 2.5mM, 3.75mM,7.5mM, and 75mM, respectively, and 1ml of sterile water was added to the first compartment of the control group, wherein mM means mmol/L.
And recording the death number of the nematodes after 12h, and calculating the mortality of the nematodes. As shown in FIG. 1, the fumigating and killing effect on Meloidogyne was 100% when the concentration of 2-methylaminoethanol was 75mM,7.5mM, and 3.75mM, and the lethal middle concentration (LC) of 2-methylaminoethanol on Meloidogyne 50 ) It was 1.5mM.
As shown in FIG. 2, observation of the 2-methylaminoethanol treated nematodes under a microscope (20 x objective) showed severe disruption of the nematode gut.
Determination of direct contact killing effect of 2-methylamino ethanol with different concentrations on meloidogyne incognita
And (3) adopting a 24-pore plate to measure the contact killing effect of the 2-methylamino ethanol on the meloidogyne incognita. 950. Mu.l of 2-methylaminoethanol diluted to different concentrations were added to 24-well plates, respectively, and 50. Mu.l of a nematode suspension containing 100 larvae per well was added. And placed in an incubator at 26 ℃ for 12h. Each treatment of 5 dishes and 3 repetitions.
The concentration of 2-methylaminoethanol was 0.075mM, 0.14mM, 0.2mM, 0.35mM, 0.75mM, 3.75mM,7.5mM, and 75mM, respectively. As a control group, 1ml of sterile water was added to a 24-well plate, and mM means mmol/L.
And recording the death number of the nematodes after 12h, and calculating the mortality of the nematodes. As shown in FIG. 3, the contact killing effect on Meloidogyne incognita was 100% when the concentration of 2-methylaminoethanol was 75mM,7.5mM, and 3.75mM, and the lethal medium concentration (LC) of 2-methylaminoethanol on Meloidogyne incognita was observed under the condition of direct contact with nematodes 50 ) It was 0.14mM.
Greenhouse pot experiment for determining prevention and treatment effect of 2-methylamino ethanol on root-knot nematodes
Test materials and methods:
cucumber: variety is Zhongnong No. 6
Nematode: meloidogyne incognita (Meloidogyne incognita)
The cucumber is cultivated in a seedling cultivating plate, and after 2 true leaves grow out, the cucumber is transplanted into a small flowerpot. After 4-5 true leaves are grown in a field planting way for about one week, the collected meloidogyne incognita is diluted into 500 pieces/ml suspension, and 1ml of the suspension is evenly inoculated around the rhizosphere of each pot of cucumber. At the same time, the mixture was treated with 2-methylaminoethanol 50-fold diluted solution (1.5 mM), and 1ml of the solution was poured into each pot. In the same way, 1ml of clear water is poured at the root of the cucumber to serve as a negative control, and 1ml of 1.8% abamectin (1. Each treated 15 cucumbers. The experimental set-up was 3 biological replicates. And (5) cleaning the roots of the cucumbers 35 days after inoculation, investigating, counting the root knots, and calculating the prevention and treatment effect. The root knot inhibition rate calculation formula is as follows: root knot inhibition (%) = (number of negative control root knots-number of treated group root knots) × 100/number of negative control root knots. The results are shown in Table 1.
The result shows that after the treatment of the 2-methylamino ethanol, compared with the control, the number of the root knots of the cucumber root is obviously reduced, the control effect is 66.11 percent, and the result is not obviously different from the abamectin treatment result. The 2-methylamino ethanol can effectively prevent and treat the root-knot nematode.
TABLE 1 Meloidogyne incognita pot test
Application method of 2-methylaminoethanol in field for fumigating and killing nematodes
The 2-methylamino ethanol can be used in the idle period and the crop cultivation period. In the idle period, the plant growth regulator can be applied to the surface layer of soil, and the number of root-knot nematodes in the soil is reduced by combining a high-temperature greenhouse closing method. The plant can be applied to the soil 15-30cm in the cultivation period, and the root-knot nematode can be controlled by utilizing the upward volatilization characteristic of the plant.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (8)
1.2-methylamino ethanol for preventing and treating root-knot nematode.
2. A medicament for the control of root-knot nematodes comprising 2-methylaminoethanol.
3. The agent according to claim 2, wherein the concentration of 2-methylaminoethanol is 0.14 to 3.75mM when controlling root-knot nematodes.
4. A medicament as claimed in claim 2 or claim 3, further comprising one or more of fosthiazate, avermectin, 1, 3-dichloropropene, trichloronitromethane, methyl thiocyanate, dazomet, metam.
5. A method for the control of root-knot nematodes using a medicament according to any one of claims 2 to 4.
6. The control method according to claim 5, wherein the concentration of 2-methylaminoethanol is 0.14 to 3.75mM.
7. The method according to claim 5 or 6, wherein 2-methylaminoethanol is applied to the surface of soil during the idle period of the farming season, and the high-temperature greenhouse closing is combined for the control of the root-knot nematode.
8. The method according to claim 5 or 6, wherein the control of root-knot nematodes is carried out by applying 2-methylaminoethanol to soil 15-30cm during the period of crop cultivation.
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| CN2022102142406 | 2022-03-07 | ||
| CN202210214240 | 2022-03-07 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040254150A1 (en) * | 2003-05-12 | 2004-12-16 | Hresko Michelle Coutu | Nematicidal compositions and methods |
| WO2006060654A2 (en) * | 2004-12-01 | 2006-06-08 | Divergence, Inc. | Pesticidal compositions and methods |
| CN113100250A (en) * | 2021-04-17 | 2021-07-13 | 青岛滕润翔检测评价有限公司 | Acaricidal composition containing mitochondrial oxidative phosphorylation inhibitor and application thereof |
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- 2022-12-12 CN CN202211596946.XA patent/CN115843797A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040254150A1 (en) * | 2003-05-12 | 2004-12-16 | Hresko Michelle Coutu | Nematicidal compositions and methods |
| WO2006060654A2 (en) * | 2004-12-01 | 2006-06-08 | Divergence, Inc. | Pesticidal compositions and methods |
| CN113100250A (en) * | 2021-04-17 | 2021-07-13 | 青岛滕润翔检测评价有限公司 | Acaricidal composition containing mitochondrial oxidative phosphorylation inhibitor and application thereof |
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