CN1158335A - Method for prepn. of chitose - Google Patents
Method for prepn. of chitose Download PDFInfo
- Publication number
- CN1158335A CN1158335A CN 96101077 CN96101077A CN1158335A CN 1158335 A CN1158335 A CN 1158335A CN 96101077 CN96101077 CN 96101077 CN 96101077 A CN96101077 A CN 96101077A CN 1158335 A CN1158335 A CN 1158335A
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- shrimp
- clear water
- rinsing
- neutral
- crab
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Abstract
A process for preparing a chitosan, esp. amidoglucosan, uses the shell of shrimp and crab as raw material and includes removing meat, rinsing, removing protein and inorganic salt, removing acetyl/polymerization, drying and grinding. Its advantages are simple method and high quality. Said product can be used as raw material of health-care product to delay sanility, promote metabolism, regulate micro circulation and improve immunity.
Description
What the present invention relates to is a kind of chitosan, concretely, relate to be a kind of principal constituent be the poly-grape amine sugar (preparation method of the macromolecular compound of α-N-Glucosamine).
Chitosan of the present invention has been widely used in body-care and medical aspect, becomes the high-new technology products in the rotten world of current wind, and is described as the 6th kind of living matter of human survival.
This be because chitosan have multiple function to the human body beneficial: it can promote human body metabolism, delay senility; It also can prevent and the treatment effect of malignant tumors and play by adjusting and the improvement to the microcirculation in human body function.
Existing result of study is verified chitosan of the present invention has good anticancer, control the cancer effect, and be employed and be actually used in medical treatment.
Chitosan of the present invention can improve and improve constantly the autoimmune function of human body, and the effect that plays diseases prevention, cures the disease, it is owing to itself be a kind of macromolecular compound of positively charged, and plays a role in health care in the many hazardous and noxious substances of human body internal adsorption.
Therefore the attention object that multi-purpose formulation, health-care product just become many logists on the our times is further developed and developed to chitosan.
The main raw material of chitosan is the shell of the Crustacean in the ocean, as crab, shrimp etc.
Main production country is at present: Japan, Korea S, the U.S., Canada and some countries of Europe.The raw material that they utilized mainly is the red pawl crab of Hokkaido, Japan and Alaska snow crab.This is because of this kind animal higher cause of chitosan content on one's body, and general extraction yield can reach 30-40%.
China is a maritime nation, and a large amount of sea-food resources is also arranged, but does not have the red pawl crab in Hokkaido and Alaska snow crab.
But China is coastal a large amount of prawns, shrimp crab (being commonly called as shrimp basketry, skin skin shrimp) and swimming crab are arranged.Utilize local shrimp shell and shrimp and crab shells to extract not only aboundresources of chitosan, and to the protection environment, it is very favourable that minimizing is polluted.
But, utilize prior art, this country is produced abundant shrimp shell and shrimp and crab shells handle to extract poly-grape amine sugar, good effect and high extraction yield can not be arranged all the time.
The objective of the invention is to utilize national resource abundant shrimp shell and shrimp and crab shells, a preparation method who is particularly suitable for extracting chitosan from national shrimp shell and shrimp and crab shells is provided.
Purpose of the present invention can be achieved through the following technical solutions:
Chitosan of the present invention (chemical name: preparation method poly-grape amine sugar):
1) shrimp, shrimp crab are decaptitated, takes off meat, continue to employ its shell;
2) with the clear water rinsing of shrimp, shrimp and crab shells;
3) with the shrimp after the clear water rinsing, shrimp and crab shells, carry out deproteinated and handle, in this operation, working concentration is about 2-6%NaOH (caustic soda) will handle back shrimp, shrimp and crab shells immersion, and immersion is carried out under 85-102 ℃ of condition, and the treatment time is about 8～12 hours;
4) material after will soaking with the clear water rinsing to neutral;
5) take off inorganic salt then, in this operation:
Working concentration is about 10～20% HCl (hydrochloric acid), under 45～80 ℃ of conditions, handled 8～12 hours, and be the inorganic salts of main component with calcium to the greatest extent until taking off fully.
6) will take off material rinsing behind the inorganic salt to neutral, working concentration is about 2% Glacial acetic acid and soaked 2～4 hours then, and the material decolouring is handled to pure white;
7) material after will decolouring takes off acetyl group and handles, and carries out polymerization when handling, in this operation:
Working concentration is about 25～35% caustic soda, handles 4～6 hours under 80～100 ℃ of conditions;
8) will take off material after acetyl group/polymerization with the clear water rinsing to neutral;
9) rinsing to neutral material is carried out drying;
10) then with exsiccant material pulverize or the aqueous finished product of sour melt into;
The present invention is described in further detail below by embodiment:
| Operation | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Raw material | The shrimp shell | Shrimp and crab shells | Shrimp/shrimp and crab shells compound |
| Rinsing | Clear water | Clear water | Clear water |
| Deproteinated treatment agent title consumption time hr temperature ℃ | 2% caustic soda excessive 12 102 | 6% caustic soda excessive 8 85 | 4% caustic soda excessive 10 95 |
| Rinsing | Clear water | Clear water | Clear water |
| Take off inorganic salt treatment agent title consumption time hr temperature ℃ | 10% salt excessive acid 12 65 | 20% salt excessive acid 8 45 | 15% salt excessive acid 0 80 |
| Rinsing | Clear water | Clear water | Clear water |
| Decolorizing treatment agent title consumption time hr temperature ℃ | Excessive 2 normal temperature of 2% Glacial acetic acid | Excessive 4 normal temperature of 2% Glacial acetic acid | Excessive 3 normal temperature of 2% Glacial acetic acid |
| Rinsing | Clear water | Clear water | Clear water |
| Take off acetyl group/aggregation processing agent title consumption time hr temperature ℃ | 25% caustic soda excessive 5～7 100 | 30% caustic soda excessive 6～8 90 | 15% caustic soda excessive 4～6 80 |
| Rinsing | Clear water | Clear water | Clear water |
| Dry | Prior art | Prior art | Prior art |
| Finished product | Acid is molten | Abrasive dust | Abrasive dust |
Processing condition of the present invention and operation can be adjusted in above-mentioned scope and different orderings, for example, can decolour after going acetyl group/polymerization if desired; When adopting treatment agent to handle, also can adopt increases concentration method gradually, for example, initial concentration is fixed on low line, in the part of treatment time scope, makes it to be increased to a higher value and keeps the regular hour.
According to technology of the present invention, starting material-shrimp shell, the shrimp/shrimp and crab shells-processing of China can be purified, after polymerization, can reach following index:
Insolubles＜1%
Ash content＜3%
Molten back viscosity 1000/500c.p.s
Exploitation of the present invention is to carry out utilizing on the national resource basis, can not handle the raw material that originates in the Chinese place of production owing to directly adopt existing processes, or the requirement that does not reach use maybe can not reach above-mentioned index, therefore, technology of the present invention has solved this problem, simultaneously, also solve the raw material problem and be not subjected to the control of foreign country raw material.Chitosan price in the international market is 7800～10000 dollars one ton, of the present inventionly successfully earns foreign exchange for country can save foreign exchange.Because chitosan has function to the human body beneficial: anti-ageing, as to strengthen self immunizing power etc. thereby can be widely used in health care and medical aspect.
Claims (3)
1, a kind of preparation method of chitosan is characterized in that selecting for use shrimp shell, shrimp and crab shells:
1) shrimp, shrimp crab are decaptitated, takes off meat, continue to employ its shell;
2) with the clear water rinsing of shrimp, shrimp and crab shells;
3) with the shrimp after the clear water rinsing, shrimp and crab shells, carry out deproteinated and handle, in this operation, working concentration is about 2-6%NaOH (caustic soda) will handle back shrimp, shrimp and crab shells immersion, and immersion is carried out under 85-102 ℃ of condition, and the treatment time is about 8～12 hours;
4) material after will soaking with the clear water rinsing to neutral;
5) take off inorganic salt then, in this operation:
Working concentration is about 10～20% HCl (hydrochloric acid), under 45～80 ℃ of conditions, handled 8～12 hours, and be the inorganic salts of main component with calcium to the greatest extent until taking off fully.
6) will take off material rinsing behind the inorganic salt to neutral, working concentration is about 2% Glacial acetic acid and soaked 2～4 hours then, and the material decolouring is handled to pure white;
7) material after will decolouring takes off acetyl group and handles, and carries out polymerization when handling, in this operation:
Working concentration is about 25～35% caustic soda, handles 4～6 hours under 801～100 ℃ of conditions;
8) will take off material after acetyl group/polymerization with the clear water rinsing to neutral;
9) rinsing to neutral material is carried out drying;
10) then with exsiccant material pulverize or the aqueous finished product of sour melt into;
2, the preparation method of chitosan according to claim 1 is characterized in that described concentration increases XLO gradually from low line.
3, the preparation method of chitosan according to claim 1 and 2 is characterized in that treatment condition being retained to the designated value of time range when described concentration reaches specified high line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96101077A CN1056853C (en) | 1996-02-26 | 1996-02-26 | Method for prepn. of chitose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96101077A CN1056853C (en) | 1996-02-26 | 1996-02-26 | Method for prepn. of chitose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1158335A true CN1158335A (en) | 1997-09-03 |
| CN1056853C CN1056853C (en) | 2000-09-27 |
Family
ID=5116913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96101077A Expired - Fee Related CN1056853C (en) | 1996-02-26 | 1996-02-26 | Method for prepn. of chitose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1056853C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113896C (en) * | 1998-01-04 | 2003-07-09 | 刘荣秀 | Preparation of epidermal growth factor with marine products and relevant discard |
| CN101200505B (en) * | 2007-11-29 | 2011-01-19 | 湖北东方天琪生物工程股份有限公司 | Method for preparing high deacetylate degree and high viscosity chitosan by using shrimp shell |
| CN101249275B (en) * | 2008-01-10 | 2011-06-29 | 中国人民解放军第二军医大学 | Wound dressing capable of insulating sea water and method of preparing the same |
| US8318913B2 (en) | 2008-03-19 | 2012-11-27 | Agratech International, Inc. | Chitosan manufacturing process |
| CN109021145A (en) * | 2018-06-19 | 2018-12-18 | 南京理工大学泰州科技学院 | The method for preparing chitosan as raw material using thick chitin |
| CN112294907A (en) * | 2020-10-27 | 2021-02-02 | 青岛翼科新程生物科技有限公司 | Chlorine dioxide nano-zinc disinfection and bacteriostasis wash-free gel |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6142547A (en) * | 1984-08-03 | 1986-03-01 | Unitika Ltd | Chitin molding |
| US5010181A (en) * | 1988-03-28 | 1991-04-23 | Coughlin Robert W | Partially treated shellfish waste for removal of heavy metals from aqueous solution |
| JP3075493B2 (en) * | 1992-03-31 | 2000-08-14 | 日本化薬株式会社 | Method for producing powdered chitosan |
-
1996
- 1996-02-26 CN CN96101077A patent/CN1056853C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113896C (en) * | 1998-01-04 | 2003-07-09 | 刘荣秀 | Preparation of epidermal growth factor with marine products and relevant discard |
| CN101200505B (en) * | 2007-11-29 | 2011-01-19 | 湖北东方天琪生物工程股份有限公司 | Method for preparing high deacetylate degree and high viscosity chitosan by using shrimp shell |
| CN101249275B (en) * | 2008-01-10 | 2011-06-29 | 中国人民解放军第二军医大学 | Wound dressing capable of insulating sea water and method of preparing the same |
| US8318913B2 (en) | 2008-03-19 | 2012-11-27 | Agratech International, Inc. | Chitosan manufacturing process |
| CN109021145A (en) * | 2018-06-19 | 2018-12-18 | 南京理工大学泰州科技学院 | The method for preparing chitosan as raw material using thick chitin |
| CN112294907A (en) * | 2020-10-27 | 2021-02-02 | 青岛翼科新程生物科技有限公司 | Chlorine dioxide nano-zinc disinfection and bacteriostasis wash-free gel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1056853C (en) | 2000-09-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Applicant after: Beijing Shengmingzhiguang Science & Technology Development Co., Ltd. Applicant before: Li Zhiqiu |
|
| CB03 | Change of inventor or designer information |
Inventor after: Li Zhiqiu Inventor after: Zhang Jiandong Inventor after: Zhang Zhendong Inventor before: Zhang Jiandong Inventor before: Li Zhiqiu Inventor before: Zhang Zhendong |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: ZHANG JIANDONG; LI ZHIQIU; ZHANG ZHENDONG TO: LI ZHIQIU; ZHANG JIANDONG; ZHANG ZHENDONG |
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| SE01 | Entry into force of request for substantive examination | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Beijing Shengmingzhiguang Science & Technology Development Co., Ltd. Document name: Notice of first review |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |