CN115786119B - Isolated culture control method and system for ginseng stem cells - Google Patents
Isolated culture control method and system for ginseng stem cells Download PDFInfo
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Abstract
The invention provides a control method and a control system for isolated culture of ginseng stem cells, and relates to the technical field of ginseng stem cell culture, wherein the control system comprises an induction culture module, an adventitious root culture detection module, a culture analysis module and an isolated control module; the induction culture module comprises a plurality of induction culture boxes; the adventitious root culture detection module comprises an effective substance detection unit and a branch image acquisition unit; the culture analysis module comprises an effective substance analysis unit, an adventitious root image analysis unit and a culture effectiveness analysis unit; the separation control module is used for calculating and comparing effective culture values, and the invention can improve the control accuracy and effectiveness of the separation culture time, thereby improving the quality of artificial culture of ginseng, and solving the problem that the prior art lacks of accurate detection of the culture process of ginseng stem cells, so that the accuracy and effectiveness of separation culture control are insufficient.
Description
Technical Field
The invention relates to the technical field of ginseng stem cell culture, in particular to a method and a system for controlling isolated culture of ginseng stem cells.
Background
Ginseng is perennial herb of Panax of Araliaceae. Up to 60 cm; short rhizome, spindle shape of main root; typically under deciduous broadleaf forests or needle She Kuoshe blends at altitudes of hundreds of meters. At present, wild ginseng has fewer resources, the artificial ginseng tissue culture is usually carried out by adopting the culture technology of ginseng stem cells in the prior art, the ginseng tissue culture is not limited by seasons, and the large-scale industrial production is easy to carry out. Plant meristems can be divided into two main categories, namely apical meristems and lateral meristems, depending on their location. Wherein the apical meristem comprises a Shoot Apical Meristem (SAM) and a Root Apical Meristem (RAM). Plant stem cells exist within a special construct called meristem, with a very striking regenerative capacity.
In the prior art, since the culture of adventitious roots of ginseng stem cells is usually carried out for about one month, a certain difficulty exists in monitoring whether the culture process is effective or not in the process of inducing the culture of adventitious roots, and the existing technology generally separates and recultures the adventitious roots after the culture period is reached, and the operation is insufficient in monitoring the adventitious roots which are generated in advance or have insufficient content of effective substances after the generation, so that the problem that the separation process of some adventitious roots is delayed or the reculture effect is influenced by the insufficient content of the effective substances of the adventitious roots is caused.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a method and a system for controlling the separation culture of ginseng stem cells, which can improve the control accuracy and the effectiveness of the separation culture time by detecting and analyzing the adventitious root culture process of ginseng tissues, further improve the quality of artificial culture of ginseng, and solve the problems of the prior art that the lack of accurate detection of the culture process of the ginseng stem cells leads to the defects of the accuracy and the effectiveness of the separation culture control.
In order to achieve the above object, the present invention is realized by the following technical scheme: the control system comprises an induction culture module, an adventitious root culture detection module, a culture analysis module and a separation control module;
the induction culture module comprises a plurality of induction culture boxes, wherein the induction culture boxes are used for carrying out adventitious root culture on ginseng tissues; the induction culture module is configured with an induction culture setting strategy comprising: setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions in different proportions;
the adventitious root culture detection module comprises an effective substance detection unit and a branch image acquisition unit; the effective substance detection unit is used for detecting the content of the effective substance of adventitious roots; the branch image acquisition unit is used for acquiring a culture image of ginseng tissue;
the culture analysis module comprises an effective substance analysis unit, an adventitious root image analysis unit and a culture effectiveness analysis unit; the effective substance analysis unit is used for analyzing the content of the effective substance of the detected adventitious roots; the adventitious root image analysis unit is used for analyzing the acquired culture image of the ginseng tissue; the culture effectiveness analysis unit is used for comprehensively calculating the content of effective substances of adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator;
the separation control module is used for carrying out calculation and comparison on the effective culture values and outputting a separation culture signal of the adventitious roots based on a calculation and comparison result.
Further, the induction culture setting strategy comprises: uniformly setting the auxin and the nutrient solution as growth solution, setting the total proportion of the growth solution as 1, and setting the sum of the ratio of the auxin and the nutrient solution in the growth solution as 1;
obtaining the number of a plurality of induction incubators, setting the number of the induction incubators as n, setting the numbers of the induction incubators as i, i being a positive integer, i representing 1 to n respectively, and setting the ratio of auxin of the induction incubators to growth solution as
And the proportion of the nutrient solution to the growth solution is set to be +.>
；
The culture temperature of the induction incubators is set to be a first temperature interval, and the illumination intensity of the induction incubators is set to be a first illumination intensity interval.
Further, the active material detection unit is configured with an active material detection strategy comprising: extracting adventitious root tissue of the first volume reagent, measuring saponin content of the adventitious root tissue by colorimetry or high performance liquid chromatography, and setting as effective substance content.
Further, the branch image acquisition unit includes an image acquisition camera, and the branch image acquisition unit is configured with a branch image acquisition policy, the branch image acquisition policy including: and acquiring culture images of ginseng tissues through an image acquisition camera every first culture period.
Further, the culture effectiveness analysis unit is configured with a culture effectiveness analysis strategy including: analyzing the culture image of ginseng tissue by an adventitious root image analysis unit, and obtaining an adventitious root culture value;
when the culture value of the adventitious roots is larger than or equal to a first culture threshold value, obtaining the content of the effective substances through an effective substance detection unit;
converting the content of the effective substances into effective culture influence coefficients through an effective substance analysis unit;
calculating an effective culture influence coefficient and an adventitious root culture value through an effective culture comprehensive calculation formula to obtain an effective culture value; the effective culture comprehensive calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pyp is an effective culture value, xyp is an effective culture influence coefficient, pbp is an adventitious root culture value, k1 is a first duty ratio coefficient, k2 is a second duty ratio coefficient, both k1 and k2 are greater than zero, and k1+k2=1.
Further, the effective substance analysis unit is configured with an effective substance analysis strategy including: calculating the content of the effective substances through an effective substance analysis formula to obtain an effective culture influence coefficient;
the effective substance analysis formula is configured to:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Xyp is effective culture influence coefficient, hyx is effective substance content, and H1 isThe reference content of the effective substances, a1 is a conversion coefficient of the content of the effective substances, and the value of a1 is larger than zero.
Further, the adventitious root image analysis unit is configured with an adventitious root image analysis policy including: acquiring a culture image of ginseng tissue at the beginning of culture, and setting the culture image as an initial reference image;
performing gray processing on the initial reference image, obtaining a contour map of ginseng tissue after gray processing, and setting the contour map as an initial contour;
dividing pixel points according to a first pixel proportion, obtaining an average value of gray values of a plurality of pixel points in an initial contour, setting the average value as an initial gray reference value, setting a first gray interval value, adding the initial gray reference value to a first gray interval value, and subtracting the first gray interval value from the initial gray reference value to obtain a gray interval lower value;
carrying out gray scale processing on the culture image of the ginseng tissue obtained in the culture process, dividing pixel points according to a first pixel proportion, obtaining a region of gray values of the pixel points between a gray scale interval lower limit value and a gray scale interval upper limit value, and setting the region as a culture development region;
correspondingly placing the initial outline into a culture development area, acquiring the area of the area outside the initial outline in the culture development area, and setting the area as a development area;
calculating the development area and the area of the initial contour through an adventitious root culture calculation formula to obtain an adventitious root culture value; the adventitious root culture calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pbp is an adventitious root culture value, sfz is a development area, scl is an initial contour area, a2 is a conversion coefficient of adventitious root culture, and a2 is greater than zero.
Further, the separation control module is further configured with an adventitious root separation policy, the adventitious root separation policy including: outputting an adventitious root high-grade culture signal when the obtained effective culture value is greater than or equal to a first effective culture threshold value and the culture duration is less than a standard culture period, and setting the adventitious root high-grade culture signal as a first adventitious root isolated culture signal;
outputting medium-grade culture signals of adventitious roots when the obtained effective culture value is larger than or equal to a first effective culture threshold value and the culture time is larger than or equal to a standard culture period, and setting the medium-grade culture signals of adventitious roots as second adventitious root isolation culture signals;
outputting an adventitious root low-level culture signal when the culture time period is greater than or equal to a standard culture period and the obtained effective culture value is smaller than a first effective culture threshold value, and setting the adventitious root low-level culture signal as a third adventitious root isolated culture signal.
Further, the isolation control module is further configured with an incubator isolation selection strategy comprising: obtaining an effective culture value every second culture period;
when the effective culture value is greater than or equal to the first effective culture threshold value, setting the corresponding induction incubator as a reference incubator;
acquiring the culture time length at the moment, and setting a reference incubator as a first-stage preferred incubator when the culture time length is smaller than a standard culture period; when the culture time period is equal to or longer than the standard culture period, the reference incubator is set as the second-stage preferred incubator.
A control method for isolated culture of ginseng stem cells, the control method comprising the steps of:
step S10, setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions with different proportions;
step S20, detecting the content of effective substances of adventitious roots and acquiring a culture image of ginseng tissues;
s30, analyzing the content of the effective substances of the detected adventitious roots, and analyzing the obtained culture image of the ginseng tissue; then, comprehensively calculating the content of the effective substances of the adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator;
and S40, calculating and comparing the effective culture values, and outputting an isolated culture signal of the adventitious roots based on the result of the calculation and comparison.
The invention has the beneficial effects that: according to the invention, by setting a plurality of proportions of auxin and nutrient solution, a plurality of induction incubators are set according to the auxin and nutrient solution with different proportions, and the design can obtain the set proportion of the auxin and nutrient solution with better induction incubator after the subsequent analysis of the effectiveness of the culture of adventitious roots, thereby being beneficial to improving the culture quality of ginseng stem cells;
the invention acquires the culture image of ginseng tissue by detecting the content of the effective substances of adventitious roots; analyzing the content of the effective substances of the detected adventitious roots, and analyzing the obtained culture image of the ginseng tissue; then, comprehensively calculating the content of the effective substances of the adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator; finally, calculating and comparing the effective culture values, and outputting an adventitious root separation culture signal based on a calculation and comparison result; the design can obtain the information of the qualified adventitious roots in the culture period, so that the timeliness and accuracy of the adventitious root separation culture are improved; meanwhile, the content of the effective substances of the adventitious roots is detected, so that the nutrition content of the adventitious roots can be obtained, and the adventitious roots with higher quality can be screened out for subsequent separation culture.
Additional aspects of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
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Other features, objects and advantages of the present invention will become more apparent upon reading of the detailed description of non-limiting embodiments, given with reference to the accompanying drawings in which:
FIG. 1 is a schematic block diagram of a control system of the present invention;
FIG. 2 is a flow chart of a control method of the present invention;
FIG. 3 is a schematic view showing the division of the culture development area according to the present invention.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present invention.
Embodiments of the invention and features of the embodiments may be combined with each other without conflict.
Example 1
Referring to fig. 1, the present invention provides a system for controlling isolated culture of ginseng stem cells, which can improve the control accuracy and effectiveness of isolated culture time by detecting and analyzing the adventitious root culture process of ginseng tissue, thereby improving the quality of artificial culture of ginseng, and solving the problem of insufficient accuracy and effectiveness of isolated culture control caused by lack of accurate detection of the culture process of ginseng stem cells in the prior art.
Specifically, the control system comprises an induction culture module, an adventitious root culture detection module, a culture analysis module and a separation control module;
the induction culture module comprises a plurality of induction culture boxes, wherein the induction culture boxes are used for carrying out adventitious root culture on ginseng tissues; the induction culture module is configured with an induction culture setting strategy comprising: setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions in different proportions; several proportions of auxin and nutrient solution are provided in the medium of the induction incubator, which may be a solid medium or a liquid medium.
The induction culture setting strategy includes: uniformly setting growth hormone and nutrient solution into growth solution, setting total proportion of growth solution into 1, and setting growth hormone and nutrient solutionThe sum of the ratio of the nutrient solution in the growth solution is equal to 1; obtaining the number of a plurality of induction incubators, setting the number of the induction incubators as n, setting the numbers of the induction incubators as i, i being a positive integer, i representing 1 to n respectively, and setting the ratio of auxin of the induction incubators to growth solution as
And the proportion of the nutrient solution to the growth solution is set to be +.>
The method comprises the steps of carrying out a first treatment on the surface of the The auxin can be one or two of indoleacetic acid and ascorbic acid, and the nutrient solution can be sucrose, for example, when the auxin accounts for 0.1 in a growth solution, the nutrient solution accounts for 0.9;
setting the culture temperature of a plurality of induction incubators as a first temperature interval, wherein the first temperature interval is usually set to 22-25 ℃; setting the illumination intensity of a plurality of induction incubators as a first illumination intensity interval, wherein the illumination intensity is a physical term and refers to the luminous flux of visible light received in a unit area; units Lux (Lux or lx); the induction incubator is usually set under the dark condition, and the first illumination intensity interval is 0-0.5lx;
the adventitious root culture detection module comprises an effective substance detection unit and a branch image acquisition unit; the effective substance detection unit is used for detecting the content of the effective substance of the adventitious roots; the active material detection unit is configured with an active material detection strategy that includes: extracting adventitious root tissue of a first volume reagent, determining the content of saponin of the adventitious root tissue by a colorimetry or a high performance liquid chromatography, setting the content as an effective substance content, wherein the determination of the saponin can be obtained by a Thin Layer Chromatography (TLC), a High Performance Liquid Chromatography (HPLC) and a colorimetry in the prior art, and the colorimetry or the high performance liquid chromatography is preferably adopted in the application; the branch image acquisition unit is used for acquiring a culture image of ginseng tissue; the branch image acquisition unit comprises an image acquisition camera, and is configured with a branch image acquisition strategy, wherein the branch image acquisition strategy comprises: and acquiring culture images of ginseng tissues through an image acquisition camera every first culture period. In specific implementation, the first culture period is set to 1 day;
the culture analysis module comprises an effective substance analysis unit, an adventitious root image analysis unit and a culture effectiveness analysis unit; the effective substance analysis unit is used for analyzing the content of the effective substance of the detected adventitious roots; the effective substance analysis unit is configured with an effective substance analysis strategy including: calculating the content of the effective substances through an effective substance analysis formula to obtain an effective culture influence coefficient; the active material analysis formula is configured to:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Xyp is effective culture influence coefficient, hyx is effective substance content, H1 is effective substance reference content, a1 is conversion coefficient of effective substance content, a1 has value greater than zero, ginsenoside content in Ginseng radix is about 4%, and fibrous root content is higher than main root; in specific implementation, H1 is set to 2%, and a1 has a value of 100.
Referring to fig. 3, the adventitious root image analysis unit is configured to analyze an acquired culture image of a ginseng tissue; the culture effectiveness analysis unit is used for comprehensively calculating the content of effective substances of adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator; the adventitious root image analysis unit is configured with an adventitious root image analysis policy, which includes: acquiring a culture image of ginseng tissue at the beginning of culture, and setting the culture image as an initial reference image;
performing gray processing on the initial reference image, obtaining a contour map of ginseng tissue after gray processing, and setting the contour map as an initial contour;
dividing pixel points according to a first pixel proportion, obtaining an average value of gray values of a plurality of pixel points in an initial contour, setting the average value as an initial gray reference value, setting a first gray interval value, adding the initial gray reference value to a first gray interval value, and subtracting the first gray interval value from the initial gray reference value to obtain a gray interval lower value; according to the gray level change in the existing ginseng tissue culture process, a first gray level interval value is set, in the specific culture process, a color panel which is obviously different is set in a background area of the ginseng tissue, the first gray level interval value is usually set between 10 and 50, for example, when a pure black background area is adopted, the obtained initial gray level reference value is 80, the first gray level interval value is set to 20, the lower gray level interval limit value is 60, and the upper gray level interval limit value is 100.
Carrying out gray scale processing on the culture image of the ginseng tissue obtained in the culture process, dividing pixel points according to a first pixel proportion, obtaining a region of gray values of the pixel points between a gray scale interval lower limit value and a gray scale interval upper limit value, and setting the region as a culture development region;
correspondingly placing the initial outline into a culture development area, acquiring the area of the area outside the initial outline in the culture development area, and setting the area as a development area; the area outside the initial outline in the culture development area is set as an expansion area;
calculating the development area and the area of the initial contour through an adventitious root culture calculation formula to obtain an adventitious root culture value; the adventitious root culture calculation formula is configured as:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pbp is an adventitious root culture value, sfz is a development area, scl is an initial contour area, a2 is a conversion coefficient of adventitious root culture, and a2 is greater than zero. In the specific implementation, a2 was set to 50, and the larger the development area, the more adventitious roots were cultured.
The culture effectiveness analysis unit is configured with a culture effectiveness analysis strategy including: analyzing the culture image of ginseng tissue by an adventitious root image analysis unit, and obtaining an adventitious root culture value;
when the culture value of the adventitious roots is larger than or equal to a first culture threshold value, obtaining the content of the effective substances through an effective substance detection unit;
converting the content of the effective substances into effective culture influence coefficients through an effective substance analysis unit;
calculating an effective culture influence coefficient and an adventitious root culture value through an effective culture comprehensive calculation formula to obtain an effective culture value; the effective culture comprehensive calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pyp is an effective culture value, xyp is an effective culture influence coefficient, pbp is an adventitious root culture value, k1 is a first duty ratio coefficient, k2 is a second duty ratio coefficient, both k1 and k2 are greater than zero, and k1+k2=1; in general, if adventitious roots can be normally cultured, a certain proportion of the content of the effective substance is provided, but the content of the effective substance is high or low, so that after the culture condition of the adventitious roots is analyzed in advance, whether the cultured adventitious roots are necessary for re-culturing or not can be obtained by analyzing the content of the effective substance, if the content of the effective substance is too low, the necessity is not great, and when specifically set, k1 is set to 0.35, and k2 is set to 0.65.
The separation control module is used for calculating and comparing the effective culture values and outputting a separation culture signal of the adventitious roots based on a result of calculation and comparison. The separation control module is further configured with an adventitious root separation policy, the adventitious root separation policy including: outputting an adventitious root high-grade culture signal when the obtained effective culture value is greater than or equal to a first effective culture threshold value and the culture duration is less than a standard culture period, and setting the adventitious root high-grade culture signal as a first adventitious root isolated culture signal;
outputting medium-grade culture signals of adventitious roots when the obtained effective culture value is larger than or equal to a first effective culture threshold value and the culture time is larger than or equal to a standard culture period, and setting the medium-grade culture signals of adventitious roots as second adventitious root isolation culture signals;
outputting an adventitious root low-level culture signal when the culture time period is greater than or equal to a standard culture period and the obtained effective culture value is smaller than a first effective culture threshold value, and setting the adventitious root low-level culture signal as a third adventitious root isolated culture signal. Specifically, in the implementation process, when the first adventitious root isolation and culture signal and the second adventitious root isolation and culture signal are output, the adventitious roots cultured in the induction incubator can be isolated and cultured, and when the third adventitious root isolation and culture signal is output, the isolation and culture is not recommended or not performed.
The isolation control module is further configured with an incubator isolation selection strategy comprising: obtaining an effective culture value every second culture period; in specific implementation, the second culture period is set to 3-5 days;
when the effective culture value is greater than or equal to the first effective culture threshold value, setting the corresponding induction incubator as a reference incubator;
acquiring the culture time length at the moment, and setting a reference incubator as a first-stage preferred incubator when the culture time length is smaller than a standard culture period; when the culture time period is equal to or longer than the standard culture period, the reference incubator is set as the second-stage preferred incubator. The incubation period was timed from the start of incubation and typically a standard incubation period was 30 days.
Example two
Referring to fig. 2, the invention further provides a control method for isolated culture of ginseng stem cells, specifically, the control method comprises the following steps:
step S10, setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions with different proportions; step S10 further comprises the following sub-steps:
step S101, uniformly setting the growth factors and the nutrient solution into a growth solution, setting the total proportion of the growth solution into 1, and setting the sum of the ratio of the growth factors and the nutrient solution in the growth solution to be equal to 1;
step S102, obtaining the number of a plurality of induction incubators, setting the number of the induction incubators as n, setting the numbers of the induction incubators as i, i being a positive integer, i representing 1 to n respectively, and setting the ratio of auxin of the induction incubators to growth solution as
And the proportion of the nutrient solution to the growth solution is set to be +.>
；
Step S103, setting the culture temperatures of the induction incubators as a first temperature interval, and setting the illumination intensities of the induction incubators as a first illumination intensity interval.
Step S20, detecting the content of effective substances of adventitious roots and acquiring a culture image of ginseng tissues; step S20 further includes the steps of:
step S2011, extracting adventitious root tissue of a first volume reagent, measuring the content of saponin of the adventitious root tissue by a colorimetry or a high performance liquid chromatography, and setting the content as the content of effective substances;
in step S2021, a culture image of ginseng tissue is acquired by the image capturing camera once every first culture period.
S30, analyzing the content of the effective substances of the detected adventitious roots, and analyzing the obtained culture image of the ginseng tissue; then, comprehensively calculating the content of the effective substances of the adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator;
step S30 further comprises the sub-steps of:
step S3011, analyzing the culture image of ginseng tissue by an adventitious root image analysis unit, and obtaining an adventitious root culture value;
step S3012, when the culture value of the adventitious roots is greater than or equal to a first culture threshold value, obtaining the content of effective substances;
step S3013, converting the content of the effective substances into effective culture influence coefficients;
step S3014, calculating an effective culture influence coefficient and an adventitious root culture value through an effective culture comprehensive calculation formula to obtain an effective culture value; the effective culture comprehensive calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pyp is an effective culture value, xyp is an effective culture influence coefficient, pbp is an adventitious root culture value, k1 is a first duty ratio coefficient, and k2 is a secondThe duty cycle, k1 and k2 are both greater than zero, and k1+k2=1.
Step S30 further comprises the sub-steps of:
step S3021, calculating the content of the effective substances through an effective substance analysis formula to obtain an effective culture influence coefficient;
in step S3022, the effective substance analysis formula is configured to:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Xyp is an effective culture influence coefficient, hyx is an effective substance content, H1 is an effective substance reference content, a1 is a conversion coefficient of the effective substance content, and a1 has a value greater than zero.
Step S30 further comprises the sub-steps of:
step S3031, obtaining a culture image of ginseng tissue at the beginning of culture and setting the culture image as an initial reference image;
step S3032, gray processing is carried out on the initial reference image, and a contour map of ginseng tissue after gray processing is obtained and set as an initial contour;
step S3033, dividing pixel points according to a first pixel proportion, obtaining an average value of gray values of a plurality of pixel points in an initial contour, setting the average value as an initial gray reference value, setting a first gray interval value, adding the initial gray reference value to a first gray interval value, subtracting a first gray interval value from the initial gray reference value, and subtracting a gray interval lower limit from the first gray interval value;
step S3034, gray scale processing is carried out on the culture image of the ginseng tissue obtained in the culture process, pixel point division is carried out according to a first pixel proportion, and a region, between a gray scale interval lower limit value and a gray scale interval upper limit value, of the gray scale value of the pixel point is obtained and set as a culture development region;
step S3035, correspondingly placing the initial outline into a culture development area, obtaining the area of the area outside the initial outline in the culture development area, and setting the area as a development area;
step S3036, calculating the development area and the area of the initial contour by using an adventitious root culture calculation formula to obtain an adventitious rootA culture value; the adventitious root culture calculation formula is configured as:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pbp is an adventitious root culture value, sfz is a development area, scl is an initial contour area, a2 is a conversion coefficient of adventitious root culture, and a2 is greater than zero.
And S40, calculating and comparing the effective culture values, and outputting an isolated culture signal of the adventitious roots based on the result of the calculation and comparison.
Step S40 further comprises the sub-steps of:
step S4011, outputting an adventitious root high-level culture signal when the obtained effective culture value is greater than or equal to a first effective culture threshold value and the culture duration is less than a standard culture period, and setting the adventitious root high-level culture signal as a first adventitious root separation culture signal;
step S4012, when the obtained effective culture value is larger than or equal to a first effective culture threshold value and the culture time period is larger than or equal to a standard culture period, outputting an adventitious root intermediate culture signal, and setting the adventitious root intermediate culture signal as a second adventitious root isolated culture signal;
step S4013, when the culture time period is equal to or longer than the standard culture period, and the obtained effective culture value is smaller than the first effective culture threshold, outputting an adventitious root low-level culture signal, and setting the adventitious root low-level culture signal as a third adventitious root isolation culture signal.
Step S40 further comprises the sub-steps of:
step S4021, obtaining an effective culture value once every second culture period;
step S4022, setting a corresponding induction incubator as a reference incubator when the effective culture value is greater than or equal to a first effective culture threshold value;
step S4023, obtaining the culture time length at the moment, and setting the reference incubator as a first-stage preferred incubator when the culture time length is smaller than the standard culture period; when the culture time period is equal to or longer than the standard culture period, the reference incubator is set as the second-stage preferred incubator.
It will be appreciated by those skilled in the art that embodiments of the present invention may be provided as a method, system, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media having computer-usable program code embodied therein. The storage medium may be implemented by any type or combination of volatile or nonvolatile Memory devices, such as static random access Memory (Static Random Access Memory, SRAM), electrically erasable Programmable Read-Only Memory (Electrically Erasable Programmable Read-Only Memory, EEPROM), erasable Programmable Read-Only Memory (ErasableProgrammable Read Only Memory, EPROM), programmable Read-Only Memory (PROM), read-Only Memory (ROM), magnetic Memory, flash Memory, magnetic disk, or optical disk. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
The above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the foregoing examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention, and are intended to be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (4)
1. The control system is characterized by comprising an induction culture module, an adventitious root culture detection module, a culture analysis module and a separation control module;
the induction culture module comprises a plurality of induction culture boxes, wherein the induction culture boxes are used for carrying out adventitious root culture on ginseng tissues; the induction culture module is configured with an induction culture setting strategy comprising: setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions in different proportions;
the adventitious root culture detection module comprises an effective substance detection unit and a branch image acquisition unit; the effective substance detection unit is used for detecting the content of the effective substance of adventitious roots; the branch image acquisition unit is used for acquiring a culture image of ginseng tissue;
the culture analysis module comprises an effective substance analysis unit, an adventitious root image analysis unit and a culture effectiveness analysis unit; the effective substance analysis unit is used for analyzing the content of the effective substance of the detected adventitious roots; the adventitious root image analysis unit is used for analyzing the acquired culture image of the ginseng tissue; the culture effectiveness analysis unit is used for comprehensively calculating the content of effective substances of adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator;
the separation control module is used for calculating and comparing the effective culture values and outputting a separation culture signal of the adventitious roots based on a calculation and comparison result;
the induction culture setting strategy comprises: uniformly setting the auxin and the nutrient solution as growth solution, setting the total proportion of the growth solution as 1, and setting the sum of the ratio of the auxin and the nutrient solution in the growth solution as 1;
obtaining a plurality ofThe number of the induction incubators is set as n, the numbers of the induction incubators are set as i, i is a positive integer, i represents 1 to n respectively, and the ratio of the auxin of the induction incubators to the growth solution is set as
And the proportion of the nutrient solution to the growth solution is set to be +.>
；
Setting the culture temperatures of a plurality of induction incubators as a first temperature interval, and setting the illumination intensities of a plurality of induction incubators as a first illumination intensity interval;
the active material detection unit is configured with an active material detection strategy that includes: extracting adventitious root tissue of the first volume reagent, determining the content of saponin of the adventitious root tissue by colorimetry or high performance liquid chromatography, and setting the content as the content of effective substances;
the branch image acquisition unit comprises an image acquisition camera, and is configured with a branch image acquisition strategy comprising: obtaining a culture image of ginseng tissue through an image acquisition camera at each first culture period;
the culture effectiveness analysis unit is configured with a culture effectiveness analysis strategy comprising: analyzing the culture image of ginseng tissue by an adventitious root image analysis unit, and obtaining an adventitious root culture value;
when the culture value of the adventitious roots is larger than or equal to a first culture threshold value, obtaining the content of the effective substances through an effective substance detection unit;
converting the content of the effective substances into effective culture influence coefficients through an effective substance analysis unit;
calculating an effective culture influence coefficient and an adventitious root culture value through an effective culture comprehensive calculation formula to obtain an effective culture value; the effective culture comprehensive calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pyp is an effective culture value, xyp is an effective culture influence coefficient, pbp is an adventitious root culture value, k1 is a first duty ratio coefficient, k2 is a second duty ratio coefficient, both k1 and k2 are greater than zero, and k1+k2=1; />
The effective substance analysis unit is configured with an effective substance analysis strategy that includes: calculating the content of the effective substances through an effective substance analysis formula to obtain an effective culture influence coefficient;
the effective substance analysis formula is configured to:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Xyp is an effective culture influence coefficient, hyx is an effective substance content, H1 is an effective substance reference content, a1 is a conversion coefficient of the effective substance content, and the value of a1 is greater than zero;
the adventitious root image analysis unit is configured with an adventitious root image analysis policy, the adventitious root image analysis policy including: acquiring a culture image of ginseng tissue at the beginning of culture, and setting the culture image as an initial reference image;
performing gray processing on the initial reference image, obtaining a contour map of ginseng tissue after gray processing, and setting the contour map as an initial contour;
dividing pixel points according to a first pixel proportion, obtaining an average value of gray values of a plurality of pixel points in an initial contour, setting the average value as an initial gray reference value, setting a first gray interval value, adding the initial gray reference value to a first gray interval value, and subtracting the first gray interval value from the initial gray reference value to obtain a gray interval lower value;
carrying out gray scale processing on the culture image of the ginseng tissue obtained in the culture process, dividing pixel points according to a first pixel proportion, obtaining a region of gray values of the pixel points between a gray scale interval lower limit value and a gray scale interval upper limit value, and setting the region as a culture development region;
correspondingly placing the initial outline into a culture development area, acquiring the area of the area outside the initial outline in the culture development area, and setting the area as a development area;
calculating the development area and the area of the initial contour through an adventitious root culture calculation formula to obtain an adventitious root culture value; the adventitious root culture calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pbp is an adventitious root culture value, sfz is a development area, scl is an initial contour area, a2 is a conversion coefficient of adventitious root culture, and a2 is greater than zero.
2. The isolated culture control system of ginseng stem cells of claim 1, wherein the isolation control module is further configured with an adventitious root isolation strategy comprising: outputting an adventitious root high-grade culture signal when the obtained effective culture value is greater than or equal to a first effective culture threshold value and the culture duration is less than a standard culture period, and setting the adventitious root high-grade culture signal as a first adventitious root isolated culture signal;
outputting medium-grade culture signals of adventitious roots when the obtained effective culture value is larger than or equal to a first effective culture threshold value and the culture time is larger than or equal to a standard culture period, and setting the medium-grade culture signals of adventitious roots as second adventitious root isolation culture signals;
outputting an adventitious root low-level culture signal when the culture time period is greater than or equal to a standard culture period and the obtained effective culture value is smaller than a first effective culture threshold value, and setting the adventitious root low-level culture signal as a third adventitious root isolated culture signal.
3. The isolated culture control system of ginseng stem cells of claim 1, wherein the isolated control module is further configured with an incubator isolation selection strategy comprising: obtaining an effective culture value every second culture period;
when the effective culture value is greater than or equal to the first effective culture threshold value, setting the corresponding induction incubator as a reference incubator;
acquiring the culture time length at the moment, and setting a reference incubator as a first-stage preferred incubator when the culture time length is smaller than a standard culture period; when the culture time period is equal to or longer than the standard culture period, the reference incubator is set as the second-stage preferred incubator.
4. A control method of a system for controlling isolated culture of ginseng stem cells, characterized by comprising the steps of:
step S10, setting a plurality of proportions of growth factors and nutrient solutions, and setting a plurality of induction incubators according to the growth factors and nutrient solutions with different proportions;
step S20, detecting the content of effective substances of adventitious roots and acquiring a culture image of ginseng tissues;
s30, analyzing the content of the effective substances of the detected adventitious roots, and analyzing the obtained culture image of the ginseng tissue; then, comprehensively calculating the content of the effective substances of the adventitious roots and the analysis result of the culture image of the ginseng tissue to obtain the effective culture value of the ginseng tissue of the induction incubator;
step S40, calculating and comparing the effective culture values, and outputting an adventitious root isolated culture signal based on the result of calculation and comparison;
step S10 comprises the following sub-steps:
step S101, uniformly setting the growth factors and the nutrient solution into a growth solution, setting the total proportion of the growth solution into 1, and setting the sum of the ratio of the growth factors and the nutrient solution in the growth solution to be equal to 1;
step S102, obtaining the number of a plurality of induction incubators, setting the number of the induction incubators as n, setting the numbers of the induction incubators as i, i being a positive integer, i representing 1 to n respectively, and setting the ratio of auxin of the induction incubators to growth solution as
And the proportion of the nutrient solution to the growth solution is set to be +.>
；
Step S103, setting the culture temperatures of a plurality of induction incubators as a first temperature interval, and setting the illumination intensities of a plurality of induction incubators as a first illumination intensity interval;
step S20 comprises the following sub-steps:
step S2011, extracting adventitious root tissue of a first volume reagent, measuring the content of saponin of the adventitious root tissue by a colorimetry or a high performance liquid chromatography, and setting the content as the content of effective substances;
step S2021, acquiring a culture image of ginseng tissue through an image acquisition camera every first culture period;
step S30 comprises the following sub-steps:
step S3011, analyzing the culture image of ginseng tissue by an adventitious root image analysis unit, and obtaining an adventitious root culture value;
step S3012, when the culture value of the adventitious roots is greater than or equal to a first culture threshold value, obtaining the content of effective substances;
step S3013, converting the content of the effective substances into effective culture influence coefficients;
step S3014, calculating an effective culture influence coefficient and an adventitious root culture value through an effective culture comprehensive calculation formula to obtain an effective culture value; the effective culture comprehensive calculation formula is configured as follows:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pyp is an effective culture value, xyp is an effective culture influence coefficient, pbp is an adventitious root culture value, k1 is a first duty ratio coefficient, k2 is a second duty ratio coefficient, both k1 and k2 are greater than zero, and k1+k2=1;
step S30 further comprises the sub-steps of:
step S3021, calculating the content of the effective substances through an effective substance analysis formula to obtain an effective culture influence coefficient;
in step S3022, the effective substance analysis formula is configured to:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Xyp is an effective culture influence coefficient, hyx is an effective substance content, H1 is an effective substance reference content, a1 is a conversion coefficient of the effective substance content, and the value of a1 is greater than zero; />
Step S30 further comprises the sub-steps of:
step S3031, obtaining a culture image of ginseng tissue at the beginning of culture and setting the culture image as an initial reference image;
step S3032, gray processing is carried out on the initial reference image, and a contour map of ginseng tissue after gray processing is obtained and set as an initial contour;
step S3033, dividing pixel points according to a first pixel proportion, obtaining an average value of gray values of a plurality of pixel points in an initial contour, setting the average value as an initial gray reference value, setting a first gray interval value, adding the initial gray reference value to a first gray interval value, subtracting a first gray interval value from the initial gray reference value, and subtracting a gray interval lower limit from the first gray interval value;
step S3034, gray scale processing is carried out on the culture image of the ginseng tissue obtained in the culture process, pixel point division is carried out according to a first pixel proportion, and a region, between a gray scale interval lower limit value and a gray scale interval upper limit value, of the gray scale value of the pixel point is obtained and set as a culture development region;
step S3035, correspondingly placing the initial outline into a culture development area, obtaining the area of the area outside the initial outline in the culture development area, and setting the area as a development area;
step S3036, calculating the development area and the area of the initial contour through an adventitious root culture calculation formula to obtain an adventitious root culture value; the adventitious root culture calculation formula is configured as:
the method comprises the steps of carrying out a first treatment on the surface of the Wherein Pbp is an adventitious root culture value, sfz is a development area, scl is an initial contour area, and a2 isThe transformation coefficient of adventitious root culture, and the value of a2 is larger than zero. />
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