CN115785952A - Photosensitive peptide element for fluorescent sensing material and preparation method thereof, fluorescent sensing material and preparation method and application thereof - Google Patents
Photosensitive peptide element for fluorescent sensing material and preparation method thereof, fluorescent sensing material and preparation method and application thereof Download PDFInfo
- Publication number
- CN115785952A CN115785952A CN202211493662.8A CN202211493662A CN115785952A CN 115785952 A CN115785952 A CN 115785952A CN 202211493662 A CN202211493662 A CN 202211493662A CN 115785952 A CN115785952 A CN 115785952A
- Authority
- CN
- China
- Prior art keywords
- sensing material
- fluorescent sensing
- photosensitive
- peptide element
- peptidyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to the technical field of food safety and pesticide detection nanometer, and discloses a photosensitive peptide element for a fluorescent sensing material, a preparation method of the photosensitive peptide element, the fluorescent sensing material, a preparation method of the fluorescent sensing material and application of the fluorescent sensing material. The photosensitive peptide element comprises peptidyl materials and metal particles bridged on the peptidyl materials; the peptidyl material is provided by at least one of diphenylalanine, carbon-terminally amidated diphenylalanine, a dipeptide, and phenylalanine-histidine; the metal particles are provided by a metal ion source. The fluorescent sensing material prepared by applying the photosensitive peptide element has the advantages of good selective identification, high detection sensitivity, quick response and strong adsorption capacity on cartap, and has good practical value.
Description
Technical Field
The invention relates to the technical field of food safety and pesticide detection nanometer, in particular to a photosensitive peptide element for a fluorescent sensing material, a method for preparing the photosensitive peptide element for the fluorescent sensing material, the photosensitive peptide element for the fluorescent sensing material prepared by the method, the fluorescent sensing material, a method for preparing the fluorescent sensing material, the fluorescent sensing material prepared by the method and application of the fluorescent sensing material in detection of cartap.
Background
Nereistoxin is a natural toxin isolated from a marine annelid helminth, heteropod worm (Lumbricoereis hepododa). As an acetylcholine antagonist, the compound can inhibit Na and K from being conducted through a terminal plate membrane, thereby causing central nervous system disorder and achieving the insecticidal effect.
Cartap is a bionic pesticide derived from cartap, has high-efficiency insecticidal capacity, and is widely applied to the modern agriculture for yield protection and production increase. However, excessive use and improper application can cause residue in plant food, and after the residue is converted into nereistoxin or dihydronereistoxin, the nereistoxin or dihydronereistoxin is transferred through a food chain and accumulated in a human body, thereby causing safety risk to human health. And the total mortality rate caused by the residual non-productive nereistoxin pesticides in the environment is up to 11.6-14.0%. Therefore, the quantitative determination method has practical significance for the quantitative determination of the residue of the nereistoxin pesticide in the plant food derived from the environment.
At present, the quantitative detection of cartap residue is difficult to meet the limit of each standard and the actual detection requirement due to the higher detection limit of the cartap residue in high performance liquid chromatography, so that the method mainly depends on a large-scale precision instrument of liquid chromatography tandem mass spectrometry. The large-scale precision instrument provides high sensitivity and accuracy, and simultaneously brings a series of defects which are difficult to ignore, such as expensive instrument cost, higher detection cost, required professional personnel and incapability of carrying out on-site timely detection.
Disclosure of Invention
The invention aims to provide a photosensitive peptide element for a fluorescent sensing material and a preparation method thereof, and the fluorescent sensing material containing the photosensitive peptide element can specifically detect cartap.
The invention also aims to provide a fluorescent sensing material and a preparation method and application thereof, and the fluorescent sensing material provided by the invention has the advantages of good selective identification, high detection sensitivity, quick response and strong adsorption capacity on cartap.
In order to achieve the above objects, a first aspect of the present invention provides a photoactive peptide element for a fluorescent sensor material, the photoactive peptide element comprising a peptidyl material and metal particles bridged on the peptidyl material; the peptidyl material is provided by at least one of diphenylalanine, carbon-terminally amidated diphenylalanine, a dipeptide, and phenylalanine-histidine; the metal particles are provided by a metal ion source.
In a second aspect, the present invention provides a method for preparing a photoactive peptide element for a fluorescent sensing material, the method comprising: in the presence of a solvent I, carrying out self-assembly on a metal ion source and a peptidyl material raw material; the peptidyl material raw material is selected from at least one of diphenylalanine, C-terminal amidated diphenylalanine, histidine and phenylalanine-histidine.
In a third aspect of the present invention, there is provided a photosensitive peptide element for a fluorescent sensing material prepared by the method described in the second aspect.
In a fourth aspect, the present invention provides a method for preparing a fluorescent sensing material, the method comprising: covalently assembling the rigid assembly with the photosensitive peptide element in the presence of a solvent II and a catalyst; the photosensitive peptide element is the photosensitive peptide element for the fluorescent sensing material of the first aspect or the third aspect; the rigid component is selected from at least one of 1,4-benzenedicarboxaldehyde and 4,4' -biphenyldicarboxaldehyde.
In a fifth aspect, the present invention provides a fluorescence sensing material prepared by the method of the fourth aspect.
The sixth aspect of the invention provides the application of the fluorescent sensing material in the fifth aspect in the cartap detection.
The fluorescent sensing material provided by the invention has the advantages of good selective identification, quick response, accurate quantification, safety and environmental protection for cartap residue detection.
More specifically, the fluorescent sensing material has the advantages of direct contact sites, cascade response effect, rapid diffusion capacity and higher safety and environmental protection, and realizes rapid response and accurate quantification of cartap residue in a multi-component food matrix.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
FIG. 1 is a transmission electron micrograph of a photoactive peptide element T1 prepared in example 1.
FIG. 2 is a scanning electron micrograph of a fluorescence sensing material L1 prepared in example 1.
FIGS. 3 and 4 are graphs showing the fluorescence responses of the photosensitive peptide element T1 and the fluorescent sensing material L1 in test example 1 to the target at different adsorption times, respectively.
Fig. 5 shows the adsorption capacity of the fluorescent sensor material L1 for the target substance in test example 2.
FIG. 6 shows fluorescence intensities of the fluorescence sensing materials L1, L2, L3, and L4.
FIG. 7 shows the adsorption specificity of the fluorescence sensing material L1 to the target.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
The average particle size in the present invention means an average diameter.
As described above, the present invention provides in a first aspect a photoactive peptide element for a fluorescent sensing material, the photoactive peptide element comprising a peptidyl material and metal particles bridged on the peptidyl material; the peptidyl material is provided by at least one of diphenylalanine, carbon-terminally amidated diphenylalanine, a dipeptide, and phenylalanine-histidine; the metal particles are provided by a metal ion source.
Preferably, the metal ion in the metal ion source is selected from at least one of zinc ion, nickel ion, copper ion and cobalt ion.
More preferably, the metal ion source is selected from at least one of zinc chloride, nickel chloride, copper chloride, and cobalt nitrate.
As described above, the second aspect of the present invention provides a method for preparing a photosensitive peptide element for a fluorescent sensing material, the method comprising: in the presence of a solvent I, carrying out self-assembly on a metal ion source and a peptidyl material raw material; the peptidyl material raw material is selected from at least one of diphenylalanine, C-terminal amidated diphenylalanine, histidine and phenylalanine-histidine.
Particularly preferably, the peptidyl material starting material is diphenylalanine. The inventor of the invention finds that under the preferable conditions, the fluorescent sensing material has better selective identification and detection sensitivity on cartap, faster response and stronger adsorption capacity.
Preferably, the dosage weight ratio of the peptidyl material raw material to the metal ion source is 1: (0.01-2).
More preferably, the weight ratio of the used amount of the peptidyl material raw material to the metal ion source is 1: (0.2-1.0). The inventor of the invention finds that under the preferable condition, the fluorescent sensing material has better selective identification and detection sensitivity on cartap, and has faster response and stronger adsorption capacity.
Preferably, in the method for preparing a photoactive peptide element for a fluorescent sensing material, the metal ion in the metal ion source is selected from at least one of zinc ion, nickel ion, copper ion and cobalt ion.
Preferably, the dosage of the solvent I is 30-60 mL relative to 100mg of the peptidyl material raw material;
preferably, the solvent I contains a mixture of (1 to 100): 1 and water.
More preferably, the solvent I contains (5 to 20): 1 of anhydrous methanol and water.
Preferably, the conditions for self-assembly include: the temperature is 85-95 ℃, and the pH value is 4-12.
More preferably, the conditions for self-assembly include: the temperature is 85-95 ℃, and the pH value is 7-11.
According to a preferred embodiment, the method of "self-assembling a source of metal ions with a peptidyl material starting material in the presence of a solvent I" comprises:
s1: carrying out first mixing on part of the solvent I and the metal ion source to obtain a mixture I; secondly, mixing the rest part of the solvent I and the peptidyl material raw material to obtain a mixture II;
s2: thirdly mixing the mixture I and the mixture II to obtain a mixture III;
s3: and (3) carrying out self-assembly on the mixture III under the heating condition of a water bath.
The time for the self-assembly is not particularly limited in the present invention until the anhydrous methanol in the mixture III is evaporated to dryness.
The specific manner of the first mixing, the second mixing and the third mixing is not particularly limited, and those skilled in the art can select the mixing according to the technical means known in the art, for example, the mixing is performed for 10 to 40min at an ultrasonic frequency of 30 to 60HZ, and the present invention is not described herein again, and those skilled in the art cannot understand the limitation of the present invention.
The method for preparing the photosensitive peptide element for the fluorescent sensing material further comprises separation, drying and other post-treatment means which are conventional in the art, the invention exemplarily provides a preferred embodiment, and exemplarily, the mixture obtained after the anhydrous methanol in the mixture III is evaporated to dryness is subjected to centrifugal separation at 8000-11000 rpm, and then the separated precipitate is dried at 50-70 ℃ to obtain the photosensitive peptide element. The present invention is not described in detail herein, and those skilled in the art will not understand the present invention as limited.
As described above, the third aspect of the present invention provides a photosensitive peptide element for a fluorescent sensing material prepared by the method described in the second aspect.
Preferably, the average particle size of the photoactive peptide elements is from 2 to 20nm.
As previously mentioned, a fourth aspect of the present invention provides a method for preparing a fluorescent sensing material, the method comprising: covalently assembling the rigid assembly with the photosensitive peptide element in the presence of a solvent II and a catalyst; the photosensitive peptide element is the photosensitive peptide element for the fluorescent sensing material described in the first aspect or the third aspect; the rigid component is selected from at least one of 1,4-benzenedicarboxaldehyde and 4,4' -biphenyldicarboxaldehyde.
Preferably, the weight ratio of the photosensitive peptide element, the rigid component and the catalyst is 1: (0.75-3.75): (1-10).
More preferably, the weight ratio of the photosensitive peptide element, the rigid component and the catalyst is 1: (1.25-2.75): (0.8-5). The inventor of the invention finds that under the preferable conditions, the fluorescent sensing material has better selective identification and detection sensitivity on cartap, faster response and stronger adsorption capacity.
Preferably, the catalyst is acetic acid.
Preferably, the solvent II is selected from at least one of anhydrous methanol, ethanol and water.
Preferably, the solvent II is used in an amount of 0.50 to 1.25mL relative to 1mg of the photoactive peptide element.
Preferably, the conditions for covalent assembly include: the time is 2-48 h and the temperature is 24-28 ℃.
More preferably, the conditions of the covalent assembly comprise: the time is 2-10 h and the temperature is 24-28 ℃.
More preferably, the covalent assembly is carried out under stirring conditions, the rotation speed of the stirring being from 100 to 400rpm.
According to a preferred embodiment, the method further comprises: and sequentially purifying and drying a mixture obtained after the covalent assembly of the photosensitive peptide element and the rigid assembly to obtain the fluorescent sensing material.
Preferably, the step of purifying comprises: the mixture obtained by covalently assembling the photoactive peptide element and the rigid component is mixed with ethanol.
The method for preparing the fluorescent sensing material further comprises separation, drying and other conventional post-processing means in the field, and the invention exemplarily provides a preferred embodiment, and exemplarily comprises the steps of mixing the mixture obtained after the covalent assembly with ethanol, performing centrifugal separation at 8000-11000 rpm, and then drying the separated precipitate at 50-70 ℃ to obtain the fluorescent sensing material. The present invention is not described in detail herein, and those skilled in the art will not understand the present invention as limited.
As described above, the fifth aspect of the present invention provides the fluorescence sensing material prepared by the method described in the fourth aspect.
Preferably, the average particle size of the fluorescent sensing material is 50 to 100nm.
As mentioned above, the sixth aspect of the present invention provides the use of the fluorescence sensing material according to the fifth aspect in the detection of cartap.
The fluorescent sensing material provided by the invention can be used for detecting the residual amount of cartap in food or other materials.
The fluorescence sensing material provided by the invention also has the following specific advantages:
(1) According to the invention, at least one metal ion of zinc ions, nickel ions, copper ions and cobalt ions and at least one peptidyl material selected from diphenylalanine, C-terminal amidated diphenylalanine, two-histidine and phenylalanine-histidine, especially diphenylalanine, are subjected to self-assembly to obtain peptidyl nanodots, and the peptidyl nanodots serving as the light-sensitive peptide element not only have a safe and environment-friendly synthesis strategy, but also have strong optical properties.
(2) According to the invention, the peptidyl nanodot serving as the photosensitive peptide element is covalently connected with at least one rigid component selected from 1,4-benzenedicarboxaldehyde and 4,4' -biphenyldicarboxaldehyde, and the formed peptidyl nanodot covalent assembly with a net-shaped framework structure has the advantages of direct contact site, cascade response effect, rapid diffusion capability and higher safety and environmental protection.
(3) The fluorescent sensing material created by the invention is safe and environment-friendly to synthesize, saves the preparation cost, has excellent detection performance, can quickly and sensitively detect cartap residue in food, and provides a basis for later popularization and application.
The present invention will be described in detail below by way of examples.
In the following examples, the room temperature was 26. + -. 2 ℃ unless otherwise specified.
In the following examples, various raw materials used are commercially available without specific description.
Diphenylalanine: purchased from Shanghai leaf Biotech, inc. and having a purity of 98%.
Carbon-terminal amidated diphenylalanine: purchased from Shanghai Chu peptide Biotech, inc. at > 98% purity.
A dibasic acid: purchased from Shanghai Chu peptide Biotech, inc. at > 98% purity.
Phenylalanine-histidine: purchased from Shanghai Chu peptide Biotech, inc. at > 98% purity.
1,4-benzenedicarboxaldehyde: purchased from scientific research and development technologies, ltd, jilin, and having a purity of 97%.
4,4' -biphenyldicarboxaldehyde: purchased from scientific research and development technologies, ltd, jilin, and having a purity of 97%.
The scanning electron microscope used below was model number SU8020, available from Hitachi corporation.
The following transmission electron microscope was used as a model Tecnai G2F 30, available from FEI.
Preparation example 1
To a 50mL centrifuge tube containing 75mg of diphenylalanine and 35.5mg of zinc chloride, 15mL of solvent I (anhydrous methanol/water, 9. And then placing the obtained mixed solution with the pH value of 10.8 in a water bath environment at 85 ℃ until all the anhydrous methanol in the system is volatilized. And finally, separating the precipitate by a centrifugal machine under the condition of 10000rpm, and drying in a 60 ℃ blast oven to obtain the photosensitive peptide element T1.
The photosensitive peptide element T1 was analyzed by transmission electron microscopy, and the results are shown in FIG. 1. As can be seen from FIG. 1, the average particle size of the photosensitive peptide element T1 is 2-20nm, and the photosensitive peptide element T1 is in a single-particle dispersion and has a spherical appearance.
Preparation example 2
15mL of a mixed solvent (anhydrous methanol/water, 9, 1,v/v) was added to 50mL of a centrifuge tube containing 75mg of a carbon-terminal amidated diphenylalaninyl group and 35.5mg of zinc chloride, and the mixture was dissolved by sonication (40HZ, 30min) at room temperature, and then the above 15mL of zinc chloride solution was added to the carbon-terminal amidated diphenylalaninyl solution under sonication, and sonication was continued (40HZ, 15min). And then placing the obtained mixed solution with the pH value of 10.8 in a water bath environment at 85 ℃ until all the anhydrous methanol in the system is volatilized. Finally separating the precipitate by a centrifuge, and drying in a blast oven at 60 ℃ to obtain the photosensitive peptide element T2;
the photosensitive peptide element T2 was analyzed by transmission electron microscopy, and the results were similar to those in FIG. 1.
Preparation example 3
To a 50mL centrifuge tube containing 75mg of dibasic acid and 15mg of cobalt nitrate were added 12mL of solvent I (anhydrous methanol/water, 5,v/v), dissolved by sonication at room temperature (40hz, 30min), and then the above 12mL of cobalt nitrate solution was added to the dibasic acid solution under sonication, followed by further sonication (40hz, 15min). And then placing the obtained mixed solution with the pH value of 10.8 in a water bath environment at 90 ℃ until all the anhydrous methanol in the system is volatilized completely. And finally, separating the precipitate by a centrifugal machine, and drying in a blast oven at 60 ℃ to obtain the photosensitive peptide element T3.
The photosensitive peptide element T3 was analyzed by transmission electron microscopy, and the results were similar to those in FIG. 1.
Preparation example 4
To a 50mL centrifuge tube containing 75mg of phenylalanine-histidine and 35.5mg of nickel chloride were added 20mL of each of solvent I (anhydrous methanol/water, 20, 1,v/v), sonicated at room temperature (40hz, 30min) to dissolve them, and then the above 20mL of nickel chloride solution was added to the phenylalanine-histidine solution under sonication, and sonication was continued (40hz, 15min). And then placing the obtained mixed solution with the pH value of 10.8 in a water bath environment at the temperature of 95 ℃ until all the anhydrous methanol in the system is volatilized. And finally, separating the precipitate by a centrifugal machine, and drying in a blast oven at 60 ℃ to obtain the photosensitive peptide element T4.
The photosensitive peptide element T4 was analyzed by transmission electron microscopy, and the results were similar to those in FIG. 1.
Example 1
4mg of the photoactive peptide element T1,6mg of 1,4-benzenedicarboxaldehyde, 2.5mL of absolute methanol, 10. Mu.L of acetic acid (6M) were added to a 25mL round-bottom flask, and stirred at 26 ℃ and 200rpm for 5h. And after the covalent assembly is finished, purifying the product with 5mL of ethanol for three times, and finally drying the obtained white product in a blast oven at 60 ℃ to obtain the fluorescent sensing material L1.
The fluorescence sensing material L1 was analyzed by scanning electron microscopy, and the results are shown in fig. 2. As can be seen from FIG. 2, the fluorescent sensing material is further covalently assembled into a spherical structure having an average particle diameter of 50 to 100nm.
Example 2
4mg of the photoactive peptide element T2,6mg of 4,4' -biphenyldicarboxaldehyde, 2.5mL of absolute methanol, 10. Mu.L of acetic acid (6M) were added to a 25mL round-bottom flask, and stirred at 200rpm for 5h at 24 ℃. And after the covalent assembly is finished, purifying the product with 5mL of ethanol for three times, and finally drying the obtained white product in a blast oven at 60 ℃ to obtain the fluorescent sensing material L2.
The fluorescence sensing material L2 is analyzed by a scanning electron microscope, and the result is similar to that of FIG. 2.
Example 3
4mg of the photoactive peptide element T3,8mg of 1,4-benzenedicarboxaldehyde, 2.5mL ethanol, 10. Mu.L acetic acid (6M) was added to a 25mL round-bottomed flask and placed under magnetic stirring at 28 ℃ and 400rpm for 2h. And after the covalent assembly is finished, purifying the product with 5mL of ethanol for three times, and finally drying the obtained white product in a blast oven at 60 ℃ to obtain the fluorescent sensing material L3.
The fluorescence sensing material L3 is analyzed by a scanning electron microscope, and the result is similar to that of FIG. 2.
Example 4
4mg of the photoactive peptide element T4,5mg of 4,4' -biphenyldicarboxaldehyde, 2.5mL of water, 10. Mu.L of acetic acid (6M) were added to a 25mL round-bottom flask, and the mixture was stirred at 100rpm and 26 ℃ for 10h. And after the covalent assembly is finished, purifying the product with 5mL of ethanol for three times, and finally drying the obtained white product in a blast oven at 60 ℃ to obtain the fluorescent sensing material L4.
The fluorescent sensing material L4 is analyzed by a scanning electron microscope, and the result is similar to that in FIG. 2.
Test example 1
The light sensing peptide element T1 and the fluorescent sensing material L1 prepared in the example 1 are respectively subjected to a dynamic response experiment, so that the cascade response effect and the rapid diffusion capability of the fluorescent sensing material with the net-shaped framework structure are verified.
Specifically, absolute methanol is used as a solvent, and a cartap standard solution is prepared for standby. Adding 100 muL of light-sensitive peptide element T1 (1 mg/mL) or 100 muL of fluorescent sensing material L1 (1 mg/mL) and 100 muL of cartap standard solution with the concentration of 360 mug/L or blank into a black pore plate with the volume of a single pore of 400 muL respectively, uniformly mixing, oscillating for 45min, recording the fluorescence intensity of the sample at fixed time intervals in the process, wherein the fluorescence intensity when the cartap standard solution is added is expressed as F, and the fluorescence intensity when a blank solvent is added is expressed as F 0 . Then plotting time sum F 0 The relationship curve between/F, the response equilibrium time is determined. Wherein time is the abscissa, F 0 and/F is the ordinate. The instrument parameters were set as: gain 115, excitation wavelength 360nm, emission wavelength 570nm.
Fig. 3 and 4 show the fluorescence response law of the photosensitive peptide element T1 and the fluorescent sensing material L1 to the added cartap under different oscillation time.
As can be seen from FIG. 3, F is the light sensitive peptide element T1 which is exposed to cartap for 0-9min 0 of/F over timeThe prolongation shows an upward trend, in particular, the opposite trend at 2min, due to the transient decrease of fluorescence of the photoactive peptide element T1 caused by the large unbalanced concentration of the photoactive peptide element T1 upon initial contact with cartap. And the fluorescence intensity tends to level off with further increase in time.
In contrast, as shown in FIG. 4, F of the fluorescence sensing material L1 0 the/F rises steadily and tends to be steady after 6min, the time required by reaction is greatly shortened, the reason is that the light-sensitive peptide element T1 has uniform and periodic spatial distribution due to a net-shaped framework structure obtained by covalent assembly with a rigid component, and after cartap molecules are in first contact with the light-sensitive peptide element T1, under the continuous driving of the net-shaped framework structure, more and more cartap molecules enter a framework cavity, so that cascade response of second contact, third contact and the like is initiated, and the cartap is identified rapidly and efficiently.
Test example 2
The adsorption capacity of the fluorescent sensing material L1 prepared in example 1 is measured, so that the adsorption capacity of the fluorescent sensing material with a net-shaped framework structure on cartap is verified.
Specifically, 7 groups of 5mg of fluorescent sensing materials L1 are weighed and placed in a 10mL centrifuge tube, 5mL of standard solution of cartap is added into the centrifuge tube, the concentration is 10, 15, 20, 25, 30, 35 and 40mg/L respectively, the centrifuge tube is shaken for 1h at room temperature, and the supernatant obtained after centrifugation is used for ultraviolet visible absorption spectrometry. Plotting concentration and adsorption Capacity (Q) e Mg/g) to determine the maximum adsorption capacity. Wherein the concentration is the abscissa and the adsorption capacity (Q) e Mg/g) is the ordinate. Detecting and setting parameters by an instrument: the sample volume was 900. Mu.L, and the detection wavelength was 204nm.
Fig. 5 shows the law of the adsorption amount of the fluorescent sensing material L1 to cartap with different concentrations. As can be seen from FIG. 5, in the range of 0-40mg/L, with the increase of cartap concentration, cartap is continuously diffused into the mesh-frame cavity space of the fluorescent sensing material L1 under the driving of the mesh-frame structure. When the cartap concentration is further increased, the adsorption value of the fluorescent sensing material L1 tends to be stable and is not increased, and the maximum adsorption capacity reaches 217.3mg/L.
The results of testing the photoactive peptide elements T2, T3, T4 and the fluorescent sensing materials L2, L3, L4 using methods similar to those of test examples 1 and 2 were similar to those of the photoactive peptide element T1 and the fluorescent sensing material L1, respectively, described above. The prepared fluorescent sensing material can quickly detect cartap residue, and provides a basis for rear-end popularization and application.
Test example 3
The fluorescence intensity of the fluorescence sensing materials L1, L2, L3 and L4 prepared in the above examples was measured.
Specifically, 3mg of each of the fluorescence sensing materials L1, L2, L3, and L4 was accurately weighed, placed in a 5mL centrifuge tube, 1mL of methanol was added thereto, and after uniformly dispersing the mixture by vortexing, 200 μ L of the above mixture was each pipetted and added to a black well plate having a single well volume of 400 μ L, and then the fluorescence intensity thereof was recorded (a.u.). The instrument parameters were set as: gain 115, excitation wavelength set to 390nm, emission wavelength set to 570nm.
Fig. 6 shows the fluorescence intensity of the fluorescence sensing materials L1, L2, L3, L4. As can be seen from fig. 6, the fluorescence sensing materials L1, L2, L3, and L4 all have stronger fluorescence intensity, and especially the fluorescence sensing material L1 has stronger fluorescence emission behavior.
Test example 4
Taking three pesticides, which are respectively as follows: cartap, fenvalerate and chlorantraniliprole are used as competitive analogs to verify the adsorption specificity of the fluorescent sensing material L1 to the cartap, so that the high selectivity of the fluorescent sensing material provided by the invention to the cartap is tested.
Specifically, methanol is used as a solvent to prepare a standard solution of cartap, fenvalerate and chlorantraniliprole with the concentration of 360 mu g/L. Then respectively adding 3mg of fluorescent sensing material L1 into 1mL of cartap, fenvalerate, chlorantraniliprole standard solution and methanol, uniformly mixing, oscillating for 45min, and recording the fluorescence intensity of the sample, wherein the fluorescence intensity when the pesticide standard solution is added is represented as F, and the fluorescence intensity when the blank solvent anhydrous methanol is added is represented as F 0 . Thereafter pesticide and F 0 Relationship between/FCurve line. Wherein the pesticide is abscissa, F 0 and/F is the ordinate. The instrument parameters were set as: gain 115, excitation wavelength 360nm, emission wavelength 570nm.
Fig. 7 shows the adsorption specificity of the fluorescent sensing material L1 for cartap. As can be seen from FIG. 7, compared with other pesticides, F of the fluorescence sensing material L1 to cartap 0 The value of/F is 2.20, which is much higher than F for other pesticides 0 and/F shows that the fluorescent sensing material L1 has better adsorption specificity to cartap. The fluorescence sensing material L1 and the cartap are combined through hydrogen bonds to generate effective electron transfer, and other pesticides have benzene rings or multi-benzene ring structures, so that the fluorescence sensing material L1 has stronger electron giving capacity compared with the cartap, and the fluorescence of the fluorescence sensing material L1 is difficult to effectively quench.
From the analysis, the fluorescent sensing material has the advantages of good selective identification, high detection sensitivity, quick response and strong adsorption capacity on the cartap, and has huge practical value and wide application prospect.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. A photosensitive peptide element for a fluorescent sensing material, which comprises a peptidyl material and metal particles bridged on the peptidyl material; the peptidyl material is provided by at least one of diphenylalanine, carbon-terminally amidated diphenylalanine, a dipeptide, and phenylalanine-histidine; the metal particles are provided by a metal ion source;
preferably, the metal ion in the metal ion source is selected from at least one of zinc ion, nickel ion, copper ion and cobalt ion.
2. A method for preparing a photoactive peptide element for a fluorescent sensing material, the method comprising: in the presence of a solvent I, carrying out self-assembly on a metal ion source and a peptidyl material raw material; the peptidyl material raw material is selected from at least one of diphenylalanine, C-terminal amidated diphenylalanine, histidine and phenylalanine-histidine;
and/or the dosage weight ratio of the peptidyl material raw material to the metal ion source is 1: (0.01-2);
and/or, the metal ions in the metal ion source are selected from at least one of zinc ions, nickel ions, copper ions and cobalt ions.
3. The method of claim 2, wherein the conditions for self-assembly comprise: the temperature is 50-100 ℃, and the pH value is 4-12.
4. A photosensitive peptide element for a fluorescent sensing material prepared by the method of claim 2 or 3;
preferably, the average particle size of the photoactive peptide elements is from 2 to 20nm.
5. A method of making a fluorescent sensing material, comprising: covalently assembling the rigid component and the photosensitive peptide element in the presence of a solvent II and a catalyst; the photosensitive peptide element is the photosensitive peptide element for the fluorescent sensing material as described in claim 1 or 4; the rigid member is selected from at least one of 1,4-benzenedicarboxaldehyde and 4,4' -biphenyldicarboxaldehyde.
6. The method of claim 5, wherein the photoactive peptide element, the rigid component, and the catalyst are used in a weight ratio of 1: (0.75-3.75): (1-10);
and/or, the catalyst is acetic acid.
7. The method of claim 5 or 6, wherein the conditions of the covalent assembly comprise: the time is 2-48 h and the temperature is 24-28 ℃.
8. The method of any of claims 5-7, wherein the method further comprises: and sequentially purifying and drying a mixture obtained after the covalent assembly of the photosensitive peptide element and the rigid assembly to obtain the fluorescent sensing material.
9. A fluorescence sensing material prepared by the method of any one of claims 5-8.
10. The use of the fluorescent sensing material of claim 9 for the detection of cartap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211493662.8A CN115785952B (en) | 2022-11-25 | 2022-11-25 | Light-sensitive peptide element for fluorescent sensing material, preparation method of light-sensitive peptide element, fluorescent sensing material, preparation method of fluorescent sensing material and application of fluorescent sensing material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211493662.8A CN115785952B (en) | 2022-11-25 | 2022-11-25 | Light-sensitive peptide element for fluorescent sensing material, preparation method of light-sensitive peptide element, fluorescent sensing material, preparation method of fluorescent sensing material and application of fluorescent sensing material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115785952A true CN115785952A (en) | 2023-03-14 |
| CN115785952B CN115785952B (en) | 2023-08-18 |
Family
ID=85441719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211493662.8A Active CN115785952B (en) | 2022-11-25 | 2022-11-25 | Light-sensitive peptide element for fluorescent sensing material, preparation method of light-sensitive peptide element, fluorescent sensing material, preparation method of fluorescent sensing material and application of fluorescent sensing material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115785952B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007085899A2 (en) * | 2005-07-06 | 2007-08-02 | Foamix Ltd. | Foamable arthropocidal composition for tropical application |
| CN101887057A (en) * | 2010-06-30 | 2010-11-17 | 北京联合大学生物化学工程学院 | Nano sensitive material of carbamic acid ester pesticides |
| CN104678029A (en) * | 2015-02-15 | 2015-06-03 | 电子科技大学 | Extraction and detection methods for mercury compounds in sediments |
| CN108083256A (en) * | 2017-12-28 | 2018-05-29 | 大连工业大学 | The preparation method of high fluorescence property fluorescent carbon quantum dot and its in Cr（VI）Application in detection |
| JP2020019797A (en) * | 2019-10-03 | 2020-02-06 | 住友化学株式会社 | Pest control composition and application thereof |
| WO2020199501A1 (en) * | 2019-03-29 | 2020-10-08 | 中国海洋大学 | Quick detection method for food allergens based on quantum dot fluorescence |
| AU2020102557A4 (en) * | 2020-10-01 | 2020-11-19 | South central university for nationalities | Method for detecting an organophosphorus pesticide based on a CdTe-ZnCdSe double quantum dot paper chip substrate |
| CN113480999A (en) * | 2021-07-02 | 2021-10-08 | 澳门大学 | Fluorescent metal nanocluster and preparation method and application thereof |
| CN115308178A (en) * | 2022-07-30 | 2022-11-08 | 中国农业大学 | Method for detecting dithiocarbamate pesticides |
-
2022
- 2022-11-25 CN CN202211493662.8A patent/CN115785952B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007085899A2 (en) * | 2005-07-06 | 2007-08-02 | Foamix Ltd. | Foamable arthropocidal composition for tropical application |
| CN101887057A (en) * | 2010-06-30 | 2010-11-17 | 北京联合大学生物化学工程学院 | Nano sensitive material of carbamic acid ester pesticides |
| CN104678029A (en) * | 2015-02-15 | 2015-06-03 | 电子科技大学 | Extraction and detection methods for mercury compounds in sediments |
| CN108083256A (en) * | 2017-12-28 | 2018-05-29 | 大连工业大学 | The preparation method of high fluorescence property fluorescent carbon quantum dot and its in Cr（VI）Application in detection |
| WO2020199501A1 (en) * | 2019-03-29 | 2020-10-08 | 中国海洋大学 | Quick detection method for food allergens based on quantum dot fluorescence |
| JP2020019797A (en) * | 2019-10-03 | 2020-02-06 | 住友化学株式会社 | Pest control composition and application thereof |
| AU2020102557A4 (en) * | 2020-10-01 | 2020-11-19 | South central university for nationalities | Method for detecting an organophosphorus pesticide based on a CdTe-ZnCdSe double quantum dot paper chip substrate |
| CN113480999A (en) * | 2021-07-02 | 2021-10-08 | 澳门大学 | Fluorescent metal nanocluster and preparation method and application thereof |
| CN115308178A (en) * | 2022-07-30 | 2022-11-08 | 中国农业大学 | Method for detecting dithiocarbamate pesticides |
Non-Patent Citations (1)
| Title |
|---|
| 张瑛等: "太赫兹光谱技术快速识别与检测大米中沙蚕毒素类农药残留", 《中国食品学报》, vol. 22, no. 9, pages 217 - 225 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115785952B (en) | 2023-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chullasat et al. | A facile optosensing protocol based on molecularly imprinted polymer coated on CdTe quantum dots for highly sensitive and selective amoxicillin detection | |
| Yang et al. | Controllable in situ fabrication of portable AuNP/mussel-inspired polydopamine molecularly imprinted SERS substrate for selective enrichment and recognition of phthalate plasticizers | |
| Zhang et al. | Homochiral fluorescence responsive molecularly imprinted polymer: Highly chiral enantiomer resolution and quantitative detection of L-penicillamine | |
| CN110387137B (en) | Water-soluble dye with indole hemicyanine structure and synthetic method thereof | |
| Chang et al. | Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline | |
| CN108517208A (en) | The preparation method and its Cu of rare earth ratio fluorescent probe2+Detection application | |
| CN109705111B (en) | Mercury ion detection probe and preparation method and application thereof | |
| CN104785222A (en) | Preparation method and application of chitosan composite material | |
| CN106383110B (en) | OTA chemical luminescence detection method based on nano gold mark aptamer sensor | |
| CN114636746B (en) | Pb detection2+Carboxyl ligand-induced annihilation ratio electrochemiluminescence aptamer sensing method | |
| CN105969338B (en) | A kind of SiO2- DNA nano materials and its preparation method and application | |
| CN110684014A (en) | Water-soluble fluorescent probe and nanoparticle with aggregation-induced emission effect for ovarian cancer and preparation method and application thereof | |
| CN113655046B (en) | Method for fishing active small molecules from mixed combined chemical molecule library | |
| CN111220672A (en) | Preparation method of self-enhanced electrochemiluminescence aptamer sensor for detecting Hg2+ based on energy resonance transfer | |
| Zhao et al. | Chiroptical-responsive nanoprobe for the optosensing of chiral amino acids | |
| CN115785952A (en) | Photosensitive peptide element for fluorescent sensing material and preparation method thereof, fluorescent sensing material and preparation method and application thereof | |
| CN110627737B (en) | Water-soluble benzoxazole fluorescent probe for detecting zinc ions as well as preparation method and application thereof | |
| CN117347336A (en) | Fluorescent aptamer biosensor for detecting kanamycin and preparation method and application thereof | |
| CN114636743B (en) | SiO-based 2 Encapsulated MAPB QDs molecular imprinting electrochemical luminescence sensor, and preparation method and application thereof | |
| CN112611739B (en) | Preparation method and detection application of visual detection of chlorphenamine maleate fluorescent test paper | |
| Xu et al. | Ultrasensitive fluorescence detection of gonyautoxins in seawater using a novel molecularly imprinted nanoprobe | |
| Li et al. | Integration of capillary electrophoresis with gold nanoparticle-based colorimetry | |
| CN107228851B (en) | Preparation and application of fluorescein isothiocyanate modified polydopamine composite material | |
| Gao et al. | Detection of nonfluorescent cyhalothrin in honey by a spheral SiO 2-based particle coating with thin fluorescent molecularly imprinted polymers film | |
| CN106008971A (en) | Method for preparing florescent probe polyimide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |