CN115777717A - Medicament composition and application thereof - Google Patents
Medicament composition and application thereof Download PDFInfo
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Images
Abstract
The invention discloses a medicament composition I, which comprises paclobutrazol and benzothiadiazole; the medicament composition II comprises paclobutrazol and chitosan; the application of the medicament composition I and/or the medicament composition II in improving the anti-fusarium wilt capacity of the first-generation field of the anti-fusarium wilt banana variety. According to the invention, the paclobutrazol and the resistance inducer benzothiadiazole and the like are jointly used for participating in the response and signal transmission of plants to pathogenic bacteria, the defense reaction of the plants is excited, the defense system is started, and the activity of the defense enzyme is induced to be improved, so that the capacity of preventing and controlling the blight disease of the first generation banana is effectively improved, the incidence rate and the severity of the disease are reduced, the use amount of chemical pesticides and chemical fertilizers is reduced, and the residue of agricultural products is reduced.
Description
Technical Field
The invention relates to the technical field of cultivation of blight-resistant banana variety tissue culture seedlings, and particularly relates to a medicament composition and application thereof.
Background
Banana wilt (Fusarium wilt of banana) caused by Fusarium oxysporum cubeba specialization (Fusarium oxysporum f.sp.cubense, foc) is a major limiting factor in the healthy development of the banana industry. The planting of disease-resistant varieties is a fundamental way for controlling the spread of the wilt, and the new variety of the Banana has the advantages of banana wilt resistance, high and stable yield, excellent quality, good storability and the like, is a banana variety mainly pushed in the wilt epidemic area in the rural area in China, and gradually replaces the original conventional variety susceptible to the wilt to become a new main planting variety in the current domestic banana production. Under the influence of field environment complexity, cultivation management and the like, the blight-resistant banana varieties often have the phenomena of unsatisfactory resistance performance of the first-generation field of tissue culture seedlings and failure in well controlling plant death rate, selection and acceptance of disease-resistant varieties by planters are seriously influenced, and the sustainable development process of the varieties is limited to a large extent. How to improve the capability of the first generation of the Banana Baozao tissue culture seedling to resist the adversities such as blight and the like is an important problem to be solved urgently in the healthy development of the banana industry. The prevention and control method applied in the current production still mainly adopts chemical prevention and control, so that the problems of environmental pollution and food safety are increasingly serious, the method for promoting the improvement of the blight resistance of the first-generation field of the Banana Bao tissue culture seedling is particularly urgent to find, the pollution of pesticides to the environment and agricultural products is reduced from the source, the requirements of the current green prevention and control development concept are met, and the method has important economic value and ecological significance.
The Chinese application CN112544358A cuts the stem of the first generation tissue culture seedling of the anti-fusarium wilt banana plant, puts in compound fertilizer in the core part of the cut stem to promote the generation of bud absorption, and reserves 1-2 strong buds for absorption treatment, so that the disease incidence rate of the banana fusarium wilt is lower than 8 percent, and the disease incidence rate of the first generation tissue culture seedling of the anti-fusarium wilt banana variety is greatly reduced; although the method can reduce the field disease incidence rate of banana wilt, obviously, the method is not a first generation tissue culture seedling, but a bud absorption offspring seedling (a second generation or a plurality of generations of seedlings), and the method of 'killing chicken and taking eggs' causes the waste of soil nutrients and management time and brings a large amount of economic loss. Therefore, a method for promoting the first-generation field wilt-resistant capability of the banana plantlet is urgently needed, so that the pollution of pesticides to the environment and agricultural products can be reduced, the waste of soil nutrients and management time can be reduced, and the production benefit can be improved.
Disclosure of Invention
Aiming at the technical problems, the invention provides a disease control cultivation technical method for promoting the first-generation field wilt resistance of the tissue culture seedling of the banana musae, and aims to obtain a method for remarkably inhibiting the occurrence of the wilt of the banana musae and improving the yield of the banana musae.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
a pharmaceutical composition I comprises paclobutrazol (PP 333) and Benzothiadiazole (BTH).
A pharmaceutical composition II comprises paclobutrazol (PP 333) and Chitosan (CTS).
The application of the medicament composition I and/or the medicament composition II in improving the anti-fusarium wilt capacity of the first-generation field of the anti-fusarium wilt banana variety is disclosed.
Furthermore, the banana variety resisting the blight is 'Baodao banana'.
Further, the using concentration of the paclobutrazol in the medicament composition I is 30-100mg/L, and the using concentration of the benzothiadiazole is 50-200mg/L; the using concentration is used after corresponding components are adjusted to corresponding concentrations.
Further, the using concentration of the paclobutrazol in the medicament composition II is 30-100mg/L, and the using concentration of the benzene chitosan is 50-800mg/L; the using concentration is used after the corresponding components are adjusted to the corresponding concentration.
The application method for improving the anti-fusarium wilt capacity of the first-generation field of the anti-fusarium wilt banana variety by adopting the medicament composition I and/or the medicament composition II comprises the following operation steps:
(1) Cup seedling cultivation: cultivating cup seedlings according to the banana seedling breeding technical specification, spraying pesticide on healthy banana tissue culture cup seedlings with 4 leaves and 1 heart stage growth, spraying paclobutrazol (PP 333) in the pesticide composition I or the pesticide composition II, spraying Benzothiadiazole (BTH) in the pesticide composition I or Chitosan (CTS) in the pesticide composition II every other day, uniformly spraying the saturated liquid drops on the front and back surfaces of banana leaves by using a sprayer, and spraying 95-105mL of diluent liquid once, so that the activity improvement of resistance related enzymes (superoxide dismutase SOD, peroxidase POD, catalase CAT, polyphenol oxidase PPO and phenylalanine ammonia lyase PAL) in banana seedlings can be induced, and the withering resistance of the banana seedlings can be enhanced;
(2) Cup seedling inoculation; when the banana tissue culture cup seedlings grow to 6-7 leaves, the banana tissue culture cup seedlings are dipped by using sterile scissors and have the concentration of 1 multiplied by 10 5 The conidium suspension liquid of Foc (No. 4 physiological race of banana wilt germ) with spores/mL is used for carrying out root cutting inoculation on 1-2 complete fibrous root systems of banana tissue culture cup seedlings, and after the germ invades the root systems, the defense reaction of the banana seedlings can be stimulated as soon as possible, the autoimmune system of the plants is activated, the defense mechanism is started, and the disease resistance and the stress resistance are further improved;
(3) Planting;
(4) And (3) field management: seedling stage (10-14 leaves): after planting, 1 new leaf is extracted and sprayed with the pesticide composition I; spraying the pesticide composition II in the seedling stage (15-20 leaves); spraying the medicament composition I in a vigorous growth period (21-25 leaves); spraying the medicament composition II at the early stage of flower bud differentiation (26-30 leaves), and continuously inducing by different inducing agents for multiple times and alternately to prolong the induced resistance, enhance the induced disease-resistant effect and realize the stable expression of the disease resistance;
(5) And (5) performing conventional management until the harvest time is reached, thus obtaining the fertilizer.
Preferably, in the step (1), the medicament composition I is 30mg/L of PP333 (paclobutrazol) +50mg/L of BTH (benzothiadiazole); the pharmaceutical composition II is 30mg/L PP333+50mg/LCTS.
Preferably, the field planting in the step (3) is that when the banana tissue culture cup seedlings grow to 9-10 leaf ages, robust, disease and pest free and mutation free banana seedlings are selected to be transplanted to a field, and 5-10g of bacillus Shi Kucao and 5-10g of fosthiazate are planted in each field planting hole.
Preferably, the pharmaceutical composition I in the young seedling stage in the step (4) is 50mg/L PP333+100mg/LBTH; the medicine composition I in the vigorous growth phase is 100mg/L PP333+200mg/L BTH; the medicament composition II in the seedling stage is 50mg/L PP333+400mg/L CTS; the medicine composition II in the early stage of flower bud differentiation is 100mg/L PP333+800mg/L CTS.
Preferably, the general management in step (5) includes fertilization management and moisture management.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the paclobutrazol and the resistance inducer benzothiadiazole and the like are jointly used for participating in the response and signal transmission of plants to pathogenic bacteria, the defense reaction of the plants is excited, the defense system is started, and the activity of the defense enzyme is induced to be improved, so that the capacity of preventing and controlling the blight disease of the first generation banana is effectively improved, the incidence rate and the severity of the disease are reduced, the use amount of chemical pesticides and chemical fertilizers is reduced, and the residue of agricultural products is reduced.
Drawings
FIG. 1 shows that the cooperation of paclobutrazol, diazosulfide and chitosan can raise the anti-blight capability of the first generation field of anti-blight banana variety.
FIG. 2 is the change in PAL enzyme activity for each treatment.
FIG. 3 is the change in PPO enzyme activity for each treatment.
FIG. 4 shows the change in POD enzyme activity in each treatment.
FIG. 5 shows the change in SOD enzyme activity in each treatment.
FIG. 6 is the change in CAT enzyme activity for each treatment.
FIG. 7 shows the case of planting 'Bao island Banana' in an exemplary base of the Wuming district; wherein, the left side is a conventional method, and the death rate of the plant in the harvesting period is up to 23.5 percent; the right side is the method of the invention, and the death rate of the plant in the harvesting period is 1.3 percent.
FIG. 8 is a case of planting 'Banana' in an exemplary base of Longan county; wherein, the left side is the method of the invention, the death rate of the plant in the harvest period is 2.4 percent; the right side is a conventional method, and the death rate of the plant in the harvesting period is up to 18.7 percent.
Detailed Description
The following detailed description is to be read in connection with the accompanying drawings, but it is to be understood that the scope of the invention is not limited to the specific embodiments. The raw materials and reagents used in the examples were all commercially available unless otherwise specified. The compound fermentation bacteria adopted in the embodiment are commercial biological organic fertilizer, microbial fertilizer or microbial agent. The tested pathogenic bacteria are fusarium oxysporum f.sp.cubense race4, foc, which are provided by the institute of environmental and plant protection of the tropical agricultural academy of sciences in china. Paclobutrazol, benzothiadiazole, and chitosan were purchased from Shanghai Michelin Biotechnology, inc.
A pharmaceutical composition I is 30mg/L PP333 (paclobutrazol) +50mg/L BTH (benzothiadiazole), 50mg/L PP333+100mg/L BTH or 100mg/L PP333+200mg/L BTH.
A pharmaceutical composition II is 30mg/L PP333+50mg/L CTS, 50mg/L PP333+400mg/L CTS or 100mg/L PP333+800mg/L CTS.
Example 1
A method for improving the anti-blight capability of the first generation field of anti-blight banana varieties by adopting a medicament composition comprises the following operation steps:
(1) Cup seedling cultivation: cultivating cup seedlings according to the banana seedling breeding technical specification, spraying and applying a medicament composition I, namely 30mg/L PP333 (paclobutrazol) +50mg/L BTH (benzothiadiazole), to healthy banana tissue culture cup seedlings with consistent 4-leaf 1-heart stage growth, spraying the PP333 first, spraying the BTH every other day, uniformly spraying the medicament composition I to the front side and the back side of a banana leaf, wherein the front side and the back side of the banana leaf are saturated with liquid drops, and spraying 95-105mL of diluent once can induce the activity improvement of resistance related enzymes (superoxide dismutase SOD, peroxidase POD, catalase CAT, polyphenol oxidase PPO and phenylalanine ammonia lyase PAL) in a banana seedling body, and enhance the withered disease resistance of the banana seedlings;
(2) Cup seedling inoculation; when the banana tissue culture cup seedlings grow to 6-7 leaves, the banana tissue culture cup seedlings are dipped by using sterile scissors and have the concentration of 1 multiplied by 10 5 The conidium suspension liquid of Foc (No. 4 physiological race of banana wilt germ) with spores/mL is used for carrying out root cutting inoculation on 1-2 complete fibrous root systems of banana tissue culture cup seedlings, and after the germ invades the root systems, the defense reaction of the banana seedlings can be stimulated as soon as possible, the autoimmune system of the plants is activated, the defense mechanism is started, and the disease resistance and the stress resistance are further improved;
(3) Planting: when the banana tissue culture cup seedlings grow to 9-10 leaf ages, selecting strong, disease and pest free and mutation free banana seedlings to be transplanted to a field, wherein 5-10g of Shi Kucao bacillus and 5-10g of fosthiazate are placed in each planting hole;
(4) And (3) field management: seedling stage (10-14 leaves): after planting, 1 new leaf is extracted and sprayed with 50mg/L PP333+100mg/L BTH (medicament composition I); seedling stage (15-20 leaves): spraying 50mg/L PP333+400mg/L CTS (medicament composition II); vigorous growth phase (21-25 leaves): spraying 100mg/L PP333+200mg/L BTH (medicament composition I); flower bud prophase of differentiation (26-30 leaves): spraying 100mg/L PP333+800mg/L CTS (chemical composition II), and continuously inducing by different inducing agents for multiple times and alternately to prolong the induced resistance, enhance the induced disease-resistant effect and realize the stable expression of the disease resistance;
(5) And (3) performing conventional management to a harvest period, namely fertilizing management: the banana garden is integrated with water and fertilizer, the application of microbial fertilizer is increased (the dosage and the use method of the microbial fertilizer are carried out according to the specification) so as to increase the content of beneficial flora in the banana garden, and the fertilizer is scientifically applied by combining the fertilizer preserving and supplying characteristics of the banana garden soil, the property of the fertilizer and the utilization rate of the fertilizer, so that the physiological balance and the robust growth of plants are ensured, and the plants in the harvesting period are ensured to have 7-8 healthy and complete green leaves, the stress resistance of the plants is enhanced, and the disease resistance is enhanced;
water content management: irrigating by using underground water, stably keeping the water content of the banana garden soil at 60-70% (wt%), and stopping irrigating 7-10 days before harvesting;
and (3) harvesting period: the bananas can be harvested in time when the plumpness reaches more than 6 years, and the plumpness cannot exceed 7.5 years.
Example 2
A method for improving the wilt resistance of first generation banana tissue culture seedlings by adopting a medicament composition II comprises the following operation steps:
(1) Cup seedling cultivation: cultivating cup seedlings according to the banana seedling breeding technical specification, carrying out spray application on healthy banana tissue culture cup seedlings with consistent 4-leaf 1-heart-stage growth by adopting a medicament composition II, namely 30mg/L PP333 (paclobutrazol) +50mg/L CTS (chitosan), spraying PP333 firstly, spraying BTH every other day, uniformly spraying 95-105mL diluent by using a sprayer until the front and back sides of banana leaves are saturated and have liquid drops, and spraying 95-105mL diluent once, so that the activity improvement of resistance related enzymes (superoxide dismutase SOD, peroxidase POD, catalase CAT, polyphenol oxidase PPO and phenylalanine ammonia lyase PAL) in banana seedlings can be induced, and the wilt resistance of the banana seedlings is enhanced;
the rest of the operation was the same as in example 1.
Test example 1
Paclobutrazol (PP 333), salicylic Acid (SA), jasmonic Acid (JA), chitosan (CTS), benzothiadiazole (BTH);
the experiment had 10 treatments in total, each treatment was repeated 3 times, each 30 replicates;
the 5 single-dose treatments were a:30mg/L PP333; b, 50mg/L BTH; c:50mg/L SA; d:50mg/L JA; e:50mg/L CTS
The 4 dual treatments were a + B (pharmaceutical composition i, inventive example 1): 30mg/LPP333+50mg/L BTH; a + C:30mg/L PP333+50mg/L SA; a + D:30mg/LPP333+50mg/L JA; a + E (pharmaceutical composition ii, inventive example 2): 30mg/L PP333+50mg/L CTS;
spraying clear water as a Control (CK) for treatment;
spraying the healthy banana seedlings with consistent 4-leaf 1-heart-stage growth in the step (1) by adopting the single-agent and double-agent treatment, wherein the single-agent is directly and uniformly sprayed to the front and back sides of the banana leaves by a sprayer until the front and back sides are saturated and have liquid drops, the double-agent is sprayed with PP333, and SA or JA is sprayed every other day, and the rest operations are the same as those in the example 1;
of these, example 1 is a + B double dose, example 2 is a + E double dose, and likewise 30 replicates for each treatment, with 3 replicates.
Determination of induced resistance effect: in the step (2), the Foc4 germ is inoculated, then the total leaf number of the whole plant and the leaf number of each level of disease are investigated and recorded in the 30 th day, the disease leaf rate is counted, the disease index is calculated according to the grading standard, and the induced resistance effect of each medicament treatment on the banana vascular wilt is compared and analyzed; the grading standard of the blight disease of banana seedlings refers to a method Huang Yonghui (2016) (Table 1).
Disease index = ([ ∑ (number of disease strains at each stage × representative value at each stage) ]/([ total number of investigated strains × maximum value) × 100
Elicitation effect (%) = [ (control disease index-treatment disease index)/control disease index ] × 100
TABLE 1 Classification Standard of seedling-stage blight of Banana
| Disease grade | Description of |
| Level | |
| 0 | The plants have no striated or yellow leaves, healthy appearance and white bulb and pseudostem tissues |
| Level 1 | The stripe or yellow symptom appears on 1-2 leaves at the lower part of the plant, the bulb tissue is browned, |
| |
The lower part of the plant has streaks or yellowing symptoms on most leaves, and the corm has browned area |
| Grade 3 | Plant wilting, large scale stripe or etiolation symptom on most leaves, |
| 4 stage | The plant almost completely wilts and withers, and the bulb is more than 3/4 to be completely blackish brown or |
Table 2 shows that the induced disease resistance effect of different treatments on banana vascular wilt is counted, in the evaluation of prevention effect of banana seedlings, the disease index of the control reaches 0.59, the disease index of each agent treated banana seedling is reduced to different degrees, and is reduced by 18.64% -57.63%, which indicates that the agent treatment plays a role in defending the infection of banana vascular wilt pathogens and obviously inhibits the occurrence of banana vascular wilt, as shown in fig. 1. Under the 4 double-agent treatment conditions of the test, the disease index of each treated banana seedling is obviously lower than that of a control and obviously lower than that of each single-agent treatment; the A + B and A + E induced resistance effect is the best in the double-agent treatment, the control effect on banana vascular wilt reaches 57.63% and 50.85% respectively, and the control effect is obviously higher than that of the control and the two-agent treatment of A + C and A + D. The induction effect of each treatment is comprehensively compared, the induction effect of the A and B double-agent treatment is the best, and the A and E treatment also has better prevention and treatment effects.
TABLE 2 induced disease resistance effect of each treatment on banana vascular wilt
| Treatment of | Index of disease condition | Resistance inducing effect (%) |
| CK | 0.59±0.05a | 0.00±0.00d |
| A | 0.43±0.02b | 27.12±0.22c |
| B | 0.43±0.03b | 27.12±0.15c |
| C | 0.48±0.03b | 18.64±0.25c |
| D | 0.47±0.03b | 20.34±0.24c |
| E | 0.40±0.03b | 32.20±0.15b |
| A+B | 0.25±0.02c | 57.63±0.15a |
| A+C | 0.33±0.02c | 44.07±0.22b |
| A+D | 0.31±0.03c | 47.46±0.20b |
| A+E | 0.29±0.02c | 50.85±0.27a |
Test example 2
Effect of paclobutrazol, benzothiadiazole and chitosan on defensive enzyme activity of banana seedlings
Extraction and enzyme activity determination of antioxidant enzymes: samples were taken 5 times in succession on the day of spray application treatment (0 d) and after treatment at 2d, 4d, 6d and 8d in test example 1, respectively, and superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO) activity and Phenylalanine Ammonia Lyase (PAL) were measured;
(1) Phenylalanine Ammonia Lyase (PAL) activity: as shown in fig. 2, the PAL enzyme activity in the banana seedlings treated by each agent is increased, the relative increase percentage of the PAL enzyme activity in the banana seedlings after the induction treatment of A + E is increased sharply, the 2 nd day reaches the highest level in each treatment and is increased by 103.50% compared with the CK of a control group, the PAL enzyme activity in the banana seedlings after the treatment of A + B reaches the highest level in each treatment and is increased by 54.58% compared with the CK of the control group and is maintained at the highest level in each treatment at the 6d and 8d, the PAL enzyme activity in the banana seedlings after the treatment of A + B respectively increases 83.00% and 101.69% compared with the CK, and the improvement effect of the treatment of A + E, A + B on the induction of the PAL enzyme activity in the banana seedlings is obvious;
(2) The activity of peroxidase (PPO) is shown in fig. 3, the PPO activity in banana seedlings treated by each agent is obviously enhanced compared with that of the control group, the relative increase percentage of the PPO enzyme activity in banana seedlings treated by A + B reaches the highest level in each treatment at the 4 th d after induction treatment, the relative increase percentage of the PPO enzyme activity in banana seedlings treated by A + B reaches the highest level in each treatment, the relative increase percentage of the PPO enzyme activity in banana seedlings treated by A + B reaches the 300.00% compared with the CK of the control group, and the CAT enzyme activity at the 6 th d is maintained at the highest level in each treatment. The A + B treatment has obvious effect of inducing the PPO enzyme activity in the banana body;
(3) Peroxidase (POD) Activity: as shown in fig. 4, the POD activity in the banana seedlings treated by each agent is obviously enhanced compared with the control group, the relative increase percentage of the POD enzyme activity in the banana seedlings is continuously increased in the first 4d of banana seedlings after the induction treatment of a + B, and the POD enzyme activity in the banana seedlings reaches a peak period at the 4 th d, is increased by 132.61% compared with the CK of the control group and is reduced after 6d, and is increased to a higher level after 8d, and is increased by 176.96% compared with the CK, and the improvement effect of the treatment of a + B on the POD enzyme activity in the induction banana seedlings is obvious;
(4) The activity of superoxide dismutase (SOD) is shown in figure 5, the SOD enzyme activity after the medicament treatment is increased faster than that of CK group, and is higher than that of CK group in activity level, the relative increase percentage of SOD enzyme activity in banana seedlings is increased to different degrees after A + E induction treatment, and the SOD enzyme activity is compared with that of CK group in 6d reaching peak period;
(5) Catalase (CAT) activity: as shown in fig. 6, the CAT enzyme activity in banana seedlings treated by each agent was significantly enhanced compared with the control group, the relative increase percentage of CAT enzyme activity in banana seedlings after a + B induction treatment increased sharply, the 2 nd d reached the highest level in each treatment, and increased by 287.45 compared with the control group CK, the relative increase percentage of CAT enzyme activity in banana seedlings after a + E treatment increased continuously after induction treatment, the 4 th d reached the highest level in each treatment after treatment, and increased by 293.79 compared with the control group CK, the CAT enzyme activity remained at the highest level in each treatment in 6d and 8d, and increased by 254.01% and 197.77 respectively compared with CK, and the effect of a + E, A + B treatment on the increase of CAT enzyme activity in the banana seedlings was significantly improved.
Test example 3
Comparison of the results in field applications
The test is respectively carried out in 2' banana island demonstration bases in Longan county and Wuming district of south-West Guangxi city in 2020 and 2021, the test cultivation management is consistent with the technical scheme provided by the invention, and the disease condition in the harvesting period is shown in fig. 7 and 8 according to the conventional method (the conventional method is Zhang Xin and other key production technologies of banana wilt-resistant variety banana island bananas) by contrast to the cultivation management; in the field, 3 cells were respectively extracted for plant mortality index investigation at the harvest time, and the results were averaged as shown in table 3.
TABLE 3 induced disease resistance Effect of the treatments on Banana wilt
The foregoing description of specific exemplary embodiments of the invention has been presented for the purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. A pharmaceutical composition i, characterized by: the pharmaceutical composition I comprises paclobutrazol and benzothiadiazole.
2. A pharmaceutical composition ii, characterized by: the medicine composition II comprises paclobutrazol and chitosan.
3. Use of the pharmaceutical composition I and/or pharmaceutical composition II according to claim 1 or 2 for increasing the resistance of a first generation of banana varieties to blight in the field.
4. Use according to claim 3, characterized in that: the banana variety resisting the blight is 'Baodao banana'.
5. Use according to claim 3, characterized in that: the using concentration of the paclobutrazol in the medicament composition I is 30-100mg/L, and the using concentration of the benzothiadiazole is 50-200mg/L.
6. Use according to claim 3, characterized in that: the using concentration of the paclobutrazol in the medicine composition II is 30-100mg/L, and the using concentration of the benzene chitosan is 50-800mg/L.
7. The method for improving the fusarium wilt resistance of first generation field of fusarium wilt resistant banana varieties by using the pharmaceutical composition I and/or the pharmaceutical composition II as defined in claim 1 or 2, which comprises the following steps:
(1) Cup seedling cultivation: spraying and applying pesticide to 4-leaf 1-heart-stage banana tissue culture cup seedlings by adopting a pesticide composition I or a pesticide composition II, spraying paclobutrazol in the pesticide composition I or the pesticide composition II, spraying diazosulfide in the pesticide composition I or chitosan in the pesticide composition II every other day, and spraying 95-105mL of diluent for one time;
(2) Cup seedling inoculation; dipping concentration of 1 multiplied by 10 when the banana tissue culture cup seedlings grow to 6 to 7 leaf ages 5 Cutting and inoculating 1-2 complete fibrous root systems of the banana tissue culture cup seedlings by using a Foc4 conidium suspension bacterial liquid with spores/mL;
(3) Planting;
(4) Field management: and (3) seedling stage: after planting, 1 new leaf is extracted and sprayed with the pesticide composition I; spraying a medicament composition II in a seedling growing period; spraying a medicament composition I in a vigorous growth period; spraying a medicament composition II at the early stage of flower bud differentiation;
(5) And (5) performing conventional management to the harvest time to obtain the fertilizer.
8. The method of claim 7, further comprising: in the step (1), the medicine composition I is 30mg/L PP333 (paclobutrazol) +50mg/L BTH (benzothiadiazole); the medicine composition II is 30mg/L PP333+50mg/L CTS.
9. The method of claim 7, further comprising: the field planting in the step (3) is that when the banana tissue culture cup seedlings grow to 9-10 leaves, strong, disease and pest free and variation free banana seedlings are selected to be transplanted to a field, and 5-10g of bacillus Shi Kucao and 5-10g of fosthiazate are placed in each field planting hole.
10. The method of claim 7, further comprising: the medicament composition I in the seedling stage in the step (4) is 50mg/L PP333+100mg/L BTH; the medicine composition I in the vigorous growth phase is 100mg/L PP333+200mg/L BTH; the medicament composition II in the seedling stage is 50mg/L PP333+400mg/L CTS; the medicine composition II in the early stage of flower bud differentiation is 100mg/L PP333+800mg/L CTS.
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| CN104738084A (en) * | 2015-03-19 | 2015-07-01 | 盐城绿茗花木有限公司 | Heat resistance enhancer for cool-season lawn grass and preparation method thereof |
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| CN109984131A (en) * | 2017-12-31 | 2019-07-09 | 海南正业中农高科股份有限公司 | A kind of composition and application containing chitosan and paclobutrazol |
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| CN104738084A (en) * | 2015-03-19 | 2015-07-01 | 盐城绿茗花木有限公司 | Heat resistance enhancer for cool-season lawn grass and preparation method thereof |
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