CN115770320A - Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive - Google Patents
Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive Download PDFInfo
- Publication number
- CN115770320A CN115770320A CN202211596110.XA CN202211596110A CN115770320A CN 115770320 A CN115770320 A CN 115770320A CN 202211596110 A CN202211596110 A CN 202211596110A CN 115770320 A CN115770320 A CN 115770320A
- Authority
- CN
- China
- Prior art keywords
- gelatin
- phytic acid
- magnesium phosphate
- calcined
- dolomite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010459 dolomite Substances 0.000 title claims abstract description 57
- 229910000514 dolomite Inorganic materials 0.000 title claims abstract description 57
- 108010010803 Gelatin Proteins 0.000 title claims abstract description 54
- 239000008273 gelatin Substances 0.000 title claims abstract description 54
- 229920000159 gelatin Polymers 0.000 title claims abstract description 54
- 235000019322 gelatine Nutrition 0.000 title claims abstract description 54
- 235000011852 gelatine desserts Nutrition 0.000 title claims abstract description 54
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 title claims abstract description 50
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 41
- GVALZJMUIHGIMD-UHFFFAOYSA-H magnesium phosphate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O GVALZJMUIHGIMD-UHFFFAOYSA-H 0.000 title claims abstract description 37
- 239000004137 magnesium phosphate Substances 0.000 title claims abstract description 37
- 229960002261 magnesium phosphate Drugs 0.000 title claims abstract description 37
- 229910000157 magnesium phosphate Inorganic materials 0.000 title claims abstract description 37
- 235000010994 magnesium phosphates Nutrition 0.000 title claims abstract description 37
- 230000001070 adhesive effect Effects 0.000 title claims abstract description 36
- 239000000853 adhesive Substances 0.000 title claims abstract description 35
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 239000000467 phytic acid Substances 0.000 title claims abstract description 28
- 229940068041 phytic acid Drugs 0.000 title claims abstract description 28
- 235000002949 phytic acid Nutrition 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 16
- 238000001354 calcination Methods 0.000 claims description 12
- 238000000227 grinding Methods 0.000 claims description 9
- 239000002639 bone cement Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 2
- 239000011575 calcium Substances 0.000 abstract description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 3
- 230000009920 chelation Effects 0.000 abstract description 3
- 238000004132 cross linking Methods 0.000 abstract description 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract description 2
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 2
- 229910001425 magnesium ion Inorganic materials 0.000 abstract description 2
- 239000000126 substance Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 14
- 239000008367 deionised water Substances 0.000 description 14
- 229910021641 deionized water Inorganic materials 0.000 description 14
- 239000011521 glass Substances 0.000 description 14
- 239000007791 liquid phase Substances 0.000 description 14
- 239000004570 mortar (masonry) Substances 0.000 description 14
- 239000007790 solid phase Substances 0.000 description 14
- 239000000843 powder Substances 0.000 description 8
- 208000024779 Comminuted Fractures Diseases 0.000 description 7
- 238000003825 pressing Methods 0.000 description 7
- 238000003892 spreading Methods 0.000 description 7
- 238000005303 weighing Methods 0.000 description 7
- 208000010392 Bone Fractures Diseases 0.000 description 6
- 206010017076 Fracture Diseases 0.000 description 5
- 239000012634 fragment Substances 0.000 description 4
- 230000007547 defect Effects 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 208000006670 Multiple fractures Diseases 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 241000272814 Anser sp. Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 239000012790 adhesive layer Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- 230000004097 bone metabolism Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003837 high-temperature calcination Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- 230000001009 osteoporotic effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive, which is prepared by dissolving gelatin in phytic acid solution, and rapidly and completely mixing and reacting magnesium phosphate/calcined dolomite with the phytic acid/gelatin solution at room temperature; the bone adhesive prepared by using the chelation of calcium and magnesium ions in the calcined dolomite and the phytic acid and the stable crosslinking of gelatin and the phytic acid based on ionic bonds can achieve stronger bonding strength, excellent compressive strength and tensile strength, has short bonding time, good biocompatibility and biodegradability, simple preparation method and low price.
Description
Technical Field
The invention relates to a preparation method of a bone adhesive, in particular to a preparation method of a magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive.
Background
The incidence of large-area bone defects caused by trauma, tumors, bone defects, arthritis and infection is high worldwide, especially the number of highly comminuted fractures due to large external forces is increasing every year. At present, the fracture is clinically treated mainly by a conservative treatment mode or an operation, and besides a few fractures are suitable for functional reduction, the fracture still needs to be treated by the operation in most cases. At present, the method for treating the comminuted fracture is a combined mode of internal fixation and external fixation, mainly uses plates, pins, screws and other firm internal fixators at the fracture part, and places metal nails at the tail ends of the plates to fix the fracture fragments, and the mode is also always the golden standard of surgical treatment. However, the surrounding joints, such as most comminuted fractures, require anatomical reduction to meet the needs of movement to prevent osteoarthritis from occurring; the internal fixator is difficult to accurately fix broken fragments together, especially highly comminuted fractures; osteoporotic bone cannot secure the screws firmly; the internal fixator serving as an in vitro implant is easy to cause bone tissue infection at an implantation part and needs to be removed after bone healing; the problems that the metal surface often forms a false film which promotes the growth of bacteria in vivo and the like still remain to be solved. Therefore, the problem of fixation of highly comminuted fractures is an urgent problem to be solved. Clinicians operating on comminuted fracture patients find it necessary to bond comminuted fracture fragments together to secure the fragments and promote bone healing.
The dolomite after high-temperature calcination releases carbon dioxide and is converted into a mixture containing CaO and MgO. Ca provided by dolomite 2+ And Mg 2+ Is physiologically involved in bone metabolism and can stimulate osteoblast regeneration and self-repair when released from bone adhesion bioactive materials. Dolomite is used as a calcium source and a magnesium source, and has great application potential when participating in the preparation of biological materials. The phytic acid is polyhydroxy compound extracted from edible beans, has strong chelating ability to various metal ions, and has biocompatibility proved, and can be used for simultaneously calcining dolomite and Mg in skeleton 2+ 、Ca 2+ Chelation was performed. The gelatin is a natural protein with high biocompatibility extracted from collagen with high richness, and has good biocompatibility. In addition, the amino groups contained in the gelatin can generate the cross-linking effect based on ionic bonds with the hydroxyl groups in the phytic acid.
Currently, the universal glue cyanoacrylate (Qiuton, a bone adhesive and its preparation method: chinese patent, CN107343965, 2020) has been developed to provide biocompatibility and adhesive strength, but its chemical integrity results in a dense adhesive layer that does not allow ingrowth of new bone tissue and limits bone growth and bone repair to some extent. Biological bone adhesives such as hydrogel (Zhang ice goose, an organic-inorganic hybrid hydrogel bone adhesive and a preparation method thereof: chinese patent, CN113577371A, 2019.) have adhesive performance and mechanical performance, but problems of low adhesive strength, excessive expansion of the adhesive and the like can occur. According to the invention, the bone adhesive prepared by adding gelatin on the basis of magnesium phosphate/phytic acid/calcined dolomite can bond broken bones in a short time, and has strong viscosity. The prepared novel bone adhesive has good adhesive property and mechanical property, and has important significance for fixing broken bones.
Disclosure of Invention
Aiming at the defects of the prior bone adhesive, the invention provides a preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive, which is prepared by adding gelatin into phytic acid solution, and rapidly and completely mixing and reacting calcined dolomite with the phytic acid/gelatin solution at room temperature.
The technical scheme of the invention is as follows:
a preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone cement comprises the following steps:
(1) Grinding natural dolomite, sieving (200 mesh sieve), placing in a muffle furnace, heating to 800-1000 ℃, and calcining for 5h to obtain calcined dolomite;
the rate of temperature rise is 5 ℃/min;
the obtained calcined dolomite contains MgO and CaO;
(2) Placing magnesium phosphate in a muffle furnace, heating to 800-1000 ℃, and calcining for 4h to obtain calcined magnesium phosphate;
the rate of temperature rise is 5 ℃/min;
(3) Preparing phytic acid solution at 0-10 ℃, adding gelatin into the phytic acid solution, and stirring and dissolving to obtain phytic acid/gelatin solution; mixing the calcined dolomite obtained in the step (1) and the calcined magnesium phosphate obtained in the step (2), then adding the mixture into a phytic acid/gelatin solution, and uniformly mixing to obtain a bone adhesive;
the mass fraction of the phytic acid solution is 15-25%;
the mass fraction of the gelatin in the phytic acid/gelatin solution is 5-13%;
the mass ratio of the calcined dolomite to the calcined magnesium phosphate is 1: 4-20, wherein the mass ratio of the total of calcined dolomite and calcined magnesium phosphate to the phytic acid/gelatin solution is 1:45 to 55.
Compared with the existing bone adhesive, the invention has the following beneficial effects:
the bone adhesive prepared by using the chelation of calcium and magnesium ions in the calcined dolomite and the phytic acid and the ionic bond-based crosslinking of gelatin and the phytic acid can achieve stronger bonding strength, excellent compressive strength and tensile strength, has short bonding time, good biocompatibility and biodegradability, simple preparation method and low price.
Drawings
Fig. 1 is a bone of example 1.
Fig. 2 is a graph of the bond strength of bone cement at different gelatin addition levels.
Detailed Description
The invention is further described below in conjunction with specific embodiments, the advantages and features of which will become apparent as the description proceeds. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
In the following examples, natural dolomite is from Qingyang county, anhui province.
Example 1
Weighing 6.25g of 70% phytic acid solution, 1.25g of gelatin and 17.5g of deionized water, adding the gelatin and the deionized water into the 70% phytic acid solution, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.025g of natural dolomite into a muffle furnace, heating to 800 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain calcined dolomite. The calcined dolomite was ground into powder with an agate mortar, and the powder was thoroughly ground and sieved through a 200 mesh sieve. 0.475g of magnesium phosphate was placed in a muffle furnace and heated to 800 ℃ at a rate of 5 ℃/min and calcined for 4h. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib for 1min along the axis to obtain the bonded pig rib.
It can be seen from fig. 1 that the broken pig ribs are cemented.
Example 2
Weighing 6.25g of phytic acid solution with the mass fraction of 70%, 1.25g of gelatin and 17.5g of deionized water, adding the gelatin and the deionized water into the phytic acid solution with the mass fraction of 70%, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.025g of natural dolomite into a muffle furnace, heating to 900 ℃ at a heating rate of 5 ℃/min, and calcining for 5 hours to obtain the calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.475g of magnesium phosphate was placed in a muffle furnace and heated to 900 ℃ at a heating rate of 5 ℃/min and calcined for 4h. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib along the axis for 1min to obtain the bonded pig rib.
Example 3
Weighing 6.25g of 70% phytic acid solution, 1.25g of gelatin and 17.5g of deionized water, adding the gelatin and the deionized water into the 70% phytic acid solution, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.025g of natural dolomite into a muffle furnace, heating to 1000 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.475g of magnesium phosphate was placed in a muffle furnace and heated to 1000 ℃ at a heating rate of 5 ℃/min and calcined for 4h. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib along the axis for 1min to obtain the bonded pig rib.
Example 4
Weighing 6.25g of phytic acid solution with the mass fraction of 70%, 1.75g of gelatin and 17g of deionized water, adding the gelatin and the deionized water into the phytic acid solution with the mass fraction of 70%, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.04g of natural dolomite into a muffle furnace, heating to 900 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.46g of magnesium phosphate was put into a muffle furnace and heated to 900 ℃ at a heating rate of 5 ℃/min and calcined for 4 hours. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib along the axis for 1min to obtain the bonded pig rib.
Example 5
Weighing 6.25g of 70% phytic acid solution, 2.25g of gelatin and 16.5g of deionized water, adding the gelatin and the deionized water into the 70% phytic acid solution, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.055g of natural dolomite into a muffle furnace, heating to 900 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.445g of magnesium phosphate was placed in a muffle furnace and heated to 900 ℃ at a heating rate of 5 ℃/min and calcined for 4h. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib for 1min along the axis to obtain the bonded pig rib.
Example 6
Weighing 6.25g of 70% phytic acid solution, 2.75g of gelatin and 16g of deionized water, adding the gelatin and the deionized water into the 70% phytic acid solution, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.07g of natural dolomite into a muffle furnace, heating to 900 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain the calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.43g of magnesium phosphate was put into a muffle furnace and heated to 900 ℃ at a heating rate of 5 ℃/min and calcined for 4 hours. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib for 1min along the axis to obtain the bonded pig rib.
Example 7
Weighing 6.25g of 70% phytic acid solution, 3.25g of gelatin and 15.5g of deionized water, adding the gelatin and the deionized water into the 70% phytic acid solution, and stirring on a magnetic stirrer until the gelatin is completely dissolved to obtain a liquid phase substance. And (3) putting 0.085g of natural dolomite into a muffle furnace, heating to 900 ℃ at the heating rate of 5 ℃/min, and calcining for 5 hours to obtain calcined dolomite. The calcined dolomite was ground into powder with an agate mortar and thoroughly ground and sieved through a 200 mesh sieve. 0.415g of magnesium phosphate is placed in a muffle furnace and heated to 900 ℃ at a heating rate of 5 ℃/min and calcined for 4h. And fully grinding the calcined natural dolomite/magnesium phosphate in an agate mortar to obtain a solid-phase substance. And mixing the solid phase and the liquid phase, quickly stirring, curing for 15s, transferring the bone adhesive to the section of a fresh pig rib by using a glass rod, uniformly spreading the bone adhesive on the section by using the glass rod, and continuously pressing the pig rib for 1min along the axis to obtain the bonded pig rib.
Claims (5)
1. The preparation method of the magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive is characterized by comprising the following steps of:
(1) Grinding natural dolomite, sieving, placing in a muffle furnace, heating to 800-1000 ℃, and calcining for 5h to obtain calcined dolomite;
(2) Placing magnesium phosphate in a muffle furnace, heating to 800-1000 ℃, and calcining for 4h to obtain calcined magnesium phosphate;
(3) Preparing phytic acid solution at 0-10 ℃, adding gelatin into the phytic acid solution, and stirring and dissolving to obtain phytic acid/gelatin solution; and (3) mixing the calcined dolomite obtained in the step (1) and the calcined magnesium phosphate obtained in the step (2), then adding the mixture into a phytic acid/gelatin solution, and uniformly mixing to obtain the bone cement.
2. The method for preparing magnesium phosphate/phytic acid/gelatin/doloma bone cement according to claim 1, wherein the temperature increase rate in step (1) or step (2) is 5 ℃/min.
3. The method for preparing magnesium phosphate/phytic acid/gelatin/dolime bone cement according to claim 1, wherein the phytic acid solution is 15 to 25% by mass in the step (3).
4. The method for preparing magnesium phosphate/phytic acid/gelatin/doloma bone cement according to claim 1, wherein in the step (3), the mass fraction of the gelatin in the phytic acid/gelatin solution is 5 to 13%.
5. The method for preparing magnesium phosphate/phytic acid/gelatin/calcined dolomite bone cement according to claim 1, wherein, in the step (3), the mass ratio of the calcined dolomite to the calcined magnesium phosphate is 1: 4-20, wherein the mass ratio of the total of calcined dolomite and calcined magnesium phosphate to the phytic acid/gelatin solution is 1:45 to 55.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211596110.XA CN115770320A (en) | 2022-12-12 | 2022-12-12 | Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211596110.XA CN115770320A (en) | 2022-12-12 | 2022-12-12 | Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115770320A true CN115770320A (en) | 2023-03-10 |
Family
ID=85392052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211596110.XA Pending CN115770320A (en) | 2022-12-12 | 2022-12-12 | Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115770320A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA499761A (en) * | 1954-02-02 | P. Grettie Donald | Gelatin | |
| US20160175481A1 (en) * | 2013-08-09 | 2016-06-23 | Gunze Limited | Bone regeneration material kit, paste-like bone regeneration material, bone regeneration material, and bone bonding material |
| CN106496601A (en) * | 2016-10-26 | 2017-03-15 | 华南理工大学 | A kind of can be from the high intensity hydrogel and preparation method thereof into tubulose or cup-shaped |
| CN107303397A (en) * | 2016-04-20 | 2017-10-31 | 中国科学院化学研究所 | A kind of Injectable compound bone cement with bioactivity and its production and use |
| CN110354305A (en) * | 2019-07-23 | 2019-10-22 | 山东大学 | The application and the bone cement based on sodium phytate that sodium phytate prepares bone cement solidify liquid |
| CN111870738A (en) * | 2020-06-11 | 2020-11-03 | 上海蕴邦生物科技有限公司 | Bone repair material and preparation method and application thereof |
| CN112516378A (en) * | 2020-12-02 | 2021-03-19 | 浙江工业大学 | Preparation method of phytic acid/silk fibroin/calcined dolomite bone adhesive |
-
2022
- 2022-12-12 CN CN202211596110.XA patent/CN115770320A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA499761A (en) * | 1954-02-02 | P. Grettie Donald | Gelatin | |
| US20160175481A1 (en) * | 2013-08-09 | 2016-06-23 | Gunze Limited | Bone regeneration material kit, paste-like bone regeneration material, bone regeneration material, and bone bonding material |
| CN107303397A (en) * | 2016-04-20 | 2017-10-31 | 中国科学院化学研究所 | A kind of Injectable compound bone cement with bioactivity and its production and use |
| CN106496601A (en) * | 2016-10-26 | 2017-03-15 | 华南理工大学 | A kind of can be from the high intensity hydrogel and preparation method thereof into tubulose or cup-shaped |
| CN110354305A (en) * | 2019-07-23 | 2019-10-22 | 山东大学 | The application and the bone cement based on sodium phytate that sodium phytate prepares bone cement solidify liquid |
| CN111870738A (en) * | 2020-06-11 | 2020-11-03 | 上海蕴邦生物科技有限公司 | Bone repair material and preparation method and application thereof |
| CN112516378A (en) * | 2020-12-02 | 2021-03-19 | 浙江工业大学 | Preparation method of phytic acid/silk fibroin/calcined dolomite bone adhesive |
Non-Patent Citations (3)
| Title |
|---|
| MANNA HU: "Fabrication of a novel bone adhesive（crosslinked phytic acid-gelatin coordinated with magnesium phosphate and calcined dolomite, and montmorillonite） for enhancing adhesion strength and biocompatibility", JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY, vol. 104, 24 April 2024 (2024-04-24), pages 317 - 334 * |
| MEININGER SUSANNE: "Phytic acid as alternative setting retarder enhanced biological performance of dicalcium phosphate cement in vitro", SCIENTIFIC REPORTS, vol. 7, pages 558 * |
| 祁燕: "植酸螯合型羟基磷灰石骨水泥的研究", 中国优秀硕士学位论文全文数据库, pages 080 - 106 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Farrar | Bone adhesives for trauma surgery: A review of challenges and developments | |
| US6593394B1 (en) | Bioactive and osteoporotic bone cement | |
| JP2004511320A (en) | Biocompatible cement containing reactive calcium phosphate nanoparticles and methods of making and using said cement | |
| CN102274543A (en) | Magnesium-based bone filling adhesive and preparation method and use thereof | |
| CN110548171B (en) | Gelatin-based bone tissue adhesive, and preparation method and application thereof | |
| CN105903063B (en) | Magnesium-based medical bone binder material and preparation method thereof | |
| CN100531805C (en) | Injectable self-cured calcium phosphate bone-rehabilitating material and its preparing process | |
| EP3496729A1 (en) | Compositions and methods for adhesion to surfaces | |
| JP7097544B2 (en) | Hard Tissue Adhesive, Hard Tissue Adhesive Kit, and Bone Cement | |
| CN109806438B (en) | Orthopedic adhesive with function of promoting bone growth and preparation method thereof | |
| CN115770320A (en) | Preparation method of magnesium phosphate/phytic acid/gelatin/calcined dolomite bone adhesive | |
| CN109821058B (en) | Antibacterial medical bonding material for orthopedics department and preparation method thereof | |
| CN116688213A (en) | Injectable double-bionic bone adhesive for promoting fracture healing and preparation method thereof | |
| CN112516378B (en) | Preparation method of phytic acid/silk fibroin/calcined dolomite bone adhesive | |
| EP3717027B1 (en) | Composition of a calcium phosphate and an additive compound containing a phosphate or phosphonate group | |
| Ruiz et al. | A new use for self-etching resin adhesives: Cementing bone fragments | |
| Lührs et al. | An in vitro bone-to-bone adhesion test method using the compression shear test | |
| JPH07213598A (en) | Bone cement | |
| Papatheofanis et al. | Experimental use of adhesives in the repair of transverse fractures of the rat and rabbit | |
| CN117338993A (en) | Modified nano hydroxyapatite/citric acid/gelatin/calcined dolomite bone binder and preparation method thereof | |
| US20240226370A1 (en) | Soft tissue adhesive composition of alpha-tcp and phosphorylated amino acid | |
| CN115400269B (en) | Injectable bone cement and preparation method and application thereof | |
| CN109331226A (en) | A kind of modified mineralized collagen artificial bone and its preparation method and application | |
| CN112336915B (en) | Cyclic-phospho-adenyl functional self-solidifying composite bone implant and its preparation method | |
| JPS63115568A (en) | Hard tissue substitute composition of human body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |