CN115769833A - Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes - Google Patents
Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes Download PDFInfo
- Publication number
- CN115769833A CN115769833A CN202211537978.2A CN202211537978A CN115769833A CN 115769833 A CN115769833 A CN 115769833A CN 202211537978 A CN202211537978 A CN 202211537978A CN 115769833 A CN115769833 A CN 115769833A
- Authority
- CN
- China
- Prior art keywords
- flammulina velutipes
- needle mushrooms
- treatment
- low
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000004321 preservation Methods 0.000 title claims abstract description 11
- 240000006499 Flammulina velutipes Species 0.000 title abstract description 64
- 235000016640 Flammulina velutipes Nutrition 0.000 title abstract description 64
- 238000009832 plasma treatment Methods 0.000 title abstract description 13
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 44
- 238000012545 processing Methods 0.000 claims abstract description 16
- 230000006378 damage Effects 0.000 claims abstract description 13
- -1 polypropylene Polymers 0.000 claims abstract description 12
- 210000002686 mushroom body Anatomy 0.000 claims abstract description 10
- 238000004806 packaging method and process Methods 0.000 claims abstract description 9
- 241000238631 Hexapoda Species 0.000 claims abstract description 7
- 239000004743 Polypropylene Substances 0.000 claims abstract description 7
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 7
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 229920001155 polypropylene Polymers 0.000 claims abstract description 7
- 239000004698 Polyethylene Substances 0.000 claims abstract description 5
- 239000002985 plastic film Substances 0.000 claims abstract description 5
- 229920006255 plastic film Polymers 0.000 claims abstract description 5
- 229920000573 polyethylene Polymers 0.000 claims abstract description 5
- 238000003672 processing method Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 46
- 230000001954 sterilising effect Effects 0.000 abstract description 14
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 14
- 238000005516 engineering process Methods 0.000 abstract description 10
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract description 2
- 239000003440 toxic substance Substances 0.000 abstract description 2
- 238000003860 storage Methods 0.000 description 46
- 230000008859 change Effects 0.000 description 16
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 14
- 102000016938 Catalase Human genes 0.000 description 14
- 108010053835 Catalase Proteins 0.000 description 14
- 102000019197 Superoxide Dismutase Human genes 0.000 description 10
- 108010012715 Superoxide dismutase Proteins 0.000 description 10
- 239000007787 solid Substances 0.000 description 10
- 102000030523 Catechol oxidase Human genes 0.000 description 9
- 108010031396 Catechol oxidase Proteins 0.000 description 9
- 102000003992 Peroxidases Human genes 0.000 description 9
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 8
- 108040007629 peroxidase activity proteins Proteins 0.000 description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 6
- 229930003268 Vitamin C Natural products 0.000 description 6
- 230000003247 decreasing effect Effects 0.000 description 6
- 235000012055 fruits and vegetables Nutrition 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 6
- 235000019154 vitamin C Nutrition 0.000 description 6
- 239000011718 vitamin C Substances 0.000 description 6
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 229940118019 malondialdehyde Drugs 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000006866 deterioration Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 238000005057 refrigeration Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000003859 lipid peroxidation Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 230000009758 senescence Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000004320 controlled atmosphere Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
Images
Landscapes
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes, which comprises the following steps: selecting needle mushrooms which are complete in mushroom body, white in color, free of opening, diseases, insect pests and mechanical injuries, putting the needle mushrooms into a polypropylene box, and hermetically packaging the needle mushrooms by adopting a polyethylene plastic film; placing the sealed needle mushrooms in a treatment bin of a DBD-CPCS device, wherein the treatment conditions are as follows: the temperature is 4 ℃, the relative humidity is 90%, the distance between the polar plates is 29cm, the processing frequency is 60-150Hz, the processing voltage is 55-105kV, and the processing time is 60-210s, the application is a low-temperature processing and cold sterilization technology, the operation temperature is close to the room temperature, the damage effect on heat sensitive nutrients is avoided, toxic substances such as ethylene oxide and the like are also avoided, and the advantages are obvious compared with the traditional heat sterilization technology; and the sterilization time is short, and the shelf life of the flammulina velutipes can be prolonged to more than 22 days after 150 seconds of treatment.
Description
Technical Field
The invention belongs to the technical field of fresh preservation, and particularly relates to a low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes.
Background
China is a big country for producing and consuming edible fungi, and the yield accounts for more than 75 percent of the world. The golden mushroom is one of the most widely cultivated and consumed bulk edible mushrooms in China, and the total yield of the golden mushroom in the nation in 2020 reaches 228 million tons.
After being picked, the flammulina velutipes has high water content (about 90 percent), vigorous respiratory metabolism, lack of protective tissues on the surface of sporocarp, and are easy to lose water, open umbrella, elongate stipe, lignify, brown stain, putrefaction and other quality deterioration phenomena after being picked. The needle mushroom is rich in nutrition and high in water content, still has the capacity of continuing growth after being picked, the respiration effect and the transpiration effect of a fresh fruit body are severe, and meanwhile, the mushroom body is fragile and easy to suffer from germ infection and mechanical damage. If the postharvest treatment of the flammulina velutipes is improper, the flammulina velutipes can be deliquesced after 48 hours, the body of the flammulina velutipes is softened, the root of the stipe becomes dark in color and even decays, the commercial value of the flammulina velutipes is lost, and the development of the market of the flammulina velutipes is greatly influenced.
The main dominant bacteria generated in the storage process of the fresh needle mushroom mainly comprisePseudomonasBelongs to the genus of bacteria,EwingellaWhen the flammulina velutipes are infected by microorganisms, metabolism of the fresh flammulina velutipes is accelerated, so that the water content is obviously reduced, and in the middle and later storage periods, as aerobic microorganisms multiply propagate, the respiratory strength is enhanced, cell aging and autolysis are accelerated, and meanwhile, the content of malondialdehyde is increased, membrane lipid peroxidation is deepened, and phenol oxidation is accelerated to cause browning, so that the commodity value of the fresh flammulina velutipes is seriously influenced. Therefore, the method for effectively controlling the growth of the postharvest spoilage microorganisms of the flammulina velutipes and improving the storage stability is created, and has extremely high research significance and industrial value.
At present, the main preservation means of the harvested flammulina velutipes are methods such as refrigeration, controlled atmosphere storage, chemical preservative application and the like, but the methods still have the problems of neck clamping such as low efficiency, poor effect, high safety risk, low equipment level and the like in the implementation and application process, so that the storage and transportation problems of the harvested flammulina velutipes are still outstanding.
Disclosure of Invention
In order to solve the problem of quality deterioration of the flammulina velutipes after the flammulina velutipes are harvested (particularly, the flammulina velutipes are decayed due to microbial invasion), the invention finally constructs a low-temperature plasma treatment method suitable for refreshing the flammulina velutipes after the flammulina velutipes are harvested by optimizing and storing experiments by utilizing a Dielectric Barrier Discharge (DBD) low-temperature plasma Cold sterilization technology (CPCS), so that the aim of Cold sterilization and freshness preservation is fulfilled while a storage object is not contacted, and the influence of the treatment process on the nutrition and flavor quality of the flammulina velutipes is furthest reduced.
The invention provides a low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes, which comprises the following steps of:
the method comprises the following steps: selecting needle mushrooms which are complete in mushroom body, white in color, free of opening, diseases, insect pests and mechanical injuries, putting the needle mushrooms into a polypropylene box, and hermetically packaging the needle mushrooms by adopting a polyethylene plastic film;
step two: placing the sealed flammulina velutipes in a treatment bin of a DBD-CPCS device, wherein the treatment conditions are as follows: the temperature is 4 ℃, the relative humidity is 90%, the distance between the polar plates is 29cm, the processing frequency is 60-150Hz, the processing voltage is 55-105kV, and the processing time is 60-210s.
The invention also provides a low-temperature plasma treatment method suitable for the postharvest preservation of flammulina velutipes, which comprises the following steps:
the method comprises the following steps: selecting needle mushrooms which are complete in mushroom body, white in color, free of opening, diseases, insect pests and mechanical injuries, putting the needle mushrooms into a polypropylene box, and hermetically packaging the needle mushrooms by adopting a polyethylene plastic film;
step two: placing the sealed needle mushrooms in a treatment bin of a DBD-CPCS device, wherein the treatment conditions are as follows: the temperature is 4 ℃, the relative humidity is 90%, the distance between the polar plates is 29cm, the processing frequency is 150Hz, the processing voltage is 95kV, and the processing time is 150s.
Compared with the prior art, the invention has the beneficial effects that:
1. the application is a low-temperature treatment and cold sterilization technology, the operation temperature is close to room temperature, no damage effect is generated on heat-sensitive nutrients, no toxic substances such as ethylene oxide and the like are generated, and the advantages are obvious compared with the traditional hot sterilization technology; and the sterilization time is short, and the shelf life of the flammulina velutipes can be prolonged to more than 22 days after 150 seconds of treatment.
2. The fresh-keeping effect of the application is obvious, and experimental results prove that the CPCS treatment can obviously inhibit quality deterioration processes of opening the flammulina velutipes after the flammulina velutipes are picked, elongation of stipes, browning and the like, improve the activity level of the antioxidant enzyme system of the sporocarp cells, reduce the damage of free radicals and achieve the purpose of prolonging the shelf life.
The foregoing is only an overview of the technical solutions of the present invention, and in order to more clearly understand the technical solutions of the present invention, the present invention is further described below with reference to the accompanying drawings.
Drawings
FIG. 1 is a graph showing the comparison of the change in appearance of needle mushrooms of different treatment groups during the storage period of the present application; wherein A is a needle mushroom body for storing 0 d, and B is a needle mushroom body for storing 24 d;
FIG. 2 is a graph of the effect of different treatment voltages on sterilization rate in the present application;
FIG. 3 is a graph of the effect of different treatment times on sterilization rate in the present application;
FIG. 4 is a graph of the effect of different treatment frequencies on sterilization rate in the present application;
FIG. 5 is a graph showing the total number of colonies of Flammulina velutipes of different treatment groups and the variation of the sterilization rate during the storage period of the present application;
FIG. 6 is a graph showing changes in browning degree of Flammulina velutipes of different treatment groups during storage of the present application;
FIG. 7 is a graph showing the change of PPO enzyme activity of flammulina velutipes in different treatment groups during the storage period of the application;
FIG. 8 is a graph showing the change of malondialdehyde content in enoki mushrooms of different treatment groups during storage of the application;
FIG. 9 is a graph showing the change in soluble solid content of Flammulina velutipes of different treatment groups during the storage period of the present application;
FIG. 10 is a graph showing the variation of vitamin C content in Flammulina velutipes of different treatment groups during the storage period of the present application;
FIG. 11 is a graph showing the variation of the SOD enzyme activity of Flammulina velutipes of different treatment groups during the storage period of the present application;
FIG. 12 is a graph showing the CAT enzyme activity changes in different treated groups during the storage period of the present application;
FIG. 13 is a graph showing the change in POD enzyme activity of needle mushrooms of different treatment groups during storage of the present application;
FIG. 14 is a graph showing the change of the radical scavenging rate of Flammulina velutipes of different treatment groups during the storage period of the present application.
Detailed Description
In order to understand the present invention, the following examples are given to further illustrate the present invention.
In the application, the microbial indexes of the flammulina velutipes are determined by referring to a method of GB4789.2-2016 total number of microbial colonies of food.
Example 1
In the experiment, after the needle mushrooms are picked, the needle mushrooms are immediately conveyed to a refrigeration house for 4 ℃, precooled for 24 hours, and the needle mushrooms which are complete in mushroom bodies, white in color, free of opening, diseases, insect pests and mechanical injuries are selected. Weighing 50g of needle mushroom in a polypropylene packaging box (17 cm multiplied by 12cm multiplied by 3.2 cm), carrying out sealed packaging, carrying out 3 times of repeated treatment on each group, setting and controlling Dielectric Barrier Discharge (DBD) low-temperature plasma, wherein the working gas is air with the temperature of 4 ℃, the relative humidity of 90%, the distance between electrode plates is 29cm, the treatment voltage is 75kV, the treatment time is 180s, the interval time is 30s, and the treatment frequency is 60 Hz, 90 Hz, 120Hz, 150Hz, 180 Hz and 200Hz respectively.
The plasma-untreated Flammulina velutipes were used as controls to determine microbial indicators (total number of microbial colonies) of Flammulina velutipes, and the determination was repeated 3 times for each indicator, with the results shown in FIG. 1.
Example 2
The same as example 1, except that the treatment frequency was fixed at 120Hz, the treatment time was 180s, the interval time was 30s, and the treatment voltages were 55, 65, 75, 85, 95, and 105kV, respectively, and the results are shown in FIG. 2.
Example 3
The same as example 1, except that the treatment voltage was fixed at 75kV, the treatment frequency was 120Hz, the interval time was 30, and the treatment times were 60, 90, 120, 150, 180, and 210s, respectively, as shown in FIG. 3.
Example 4
Immediately transporting the harvested needle mushrooms to a refrigeration house at 4 ℃ on the same day of experiment, precooling 24h, and selecting the needle mushrooms which are complete in mushroom bodies, white in color, free of opening mushrooms, free of diseases, insect pests and mechanical injuries. Weighing 50g of flammulina velutipes in a polypropylene packaging box (17 cm × 12cm × 3.2 cm), sealing and packaging, repeating each group of treatment for 3 times, setting and controlling the treatment time of Dielectric Barrier Discharge (DBD) low-temperature plasma (working gas is air with the temperature of 4 ℃, the relative humidity of 90 percent, and the distance between polar plates is 29 cm) to be 150s, the treatment voltage is 95kV, and the treatment frequency is 150Hz. Storing with non-plasma-treated needle mushroom as control under conditions of 4 + -0.5 deg.C and 90 + -5% relative humidity, measuring growth change, quality and physiological and biochemical indexes of needle mushroom every 3d for 1 time, continuously measuring for 24d, and repeating measurement for 3 times.
After the flammulina velutipes are stored for 24 days, the appearance change of the flammulina velutipes is shown in figure 4, the total number of the colonies of the experimental group and the control group is measured by referring to the method of measuring the total number of the microbial colonies of GB4789.2-2016 food, and the result is shown in figure 5.
The browning reaction of the needle mushrooms after being stored for 24 days is shown in FIG. 6. As can be seen from FIG. 6, as the storage time increases, the browning degrees of the control group and the experimental group both increase, wherein the absorbance of the control group is higher than that of the experimental group, which indicates that the browning degree of the control group is higher than that of the experimental group, and is consistent with the observation result of appearance change, indicating that the low-temperature plasma sterilization technology treatment has an inhibitory effect on the browning reaction of flammulina velutipes.
The PPO activity of Flammulina velutipes was detected according to LS/T6124-2017 spectrophotometry for detecting wheat flour polyphenol oxidase activity, and the results are shown in FIG. 7. As can be seen from FIG. 7, the PPO activity changes of the control group and the experimental group both show a decreasing trend, and the PPO activity of the control group is obviously higher than that of the experimental group, which indicates that the PPO activity of the flammulina velutipes can be effectively reduced after the low-temperature plasma technology treatment. Polyphenol Oxidase (PPO) is a main enzyme causing enzymatic browning of fruits and vegetables, and PPO can catalyze endogenous polyphenol substances in fruits and vegetables to be oxidized to generate melanin, so that the nutrition, flavor and appearance quality of the fruits and vegetables are seriously influenced, and therefore, the low-temperature plasma technology treatment can inhibit the browning of flammulina velutipes and keep the quality of the flammulina velutipes after picking.
The content of enoki mushroom malondialdehyde MDA was detected according to GB 5009.181-2016 determination of malondialdehyde in food safety national standard food, and the result is shown in FIG. 8. As can be seen from FIG. 8, the MDA contents of the experimental group and the control group both increased with the increase of the storage time, and the MDA content of the control group was significantly higher than that of the experimental group (P) at the later stage of storage<0.05). In the initial storage period, the MDA contents of the control group and the experimental group are 7.1665 and 4.8705nmol/g respectively, and the variation of the MDA contents of the control group and the experimental group is not obvious (P>0.05 From the 12 th storage period, the change speed of the MDA content of the control group is obviously faster than that of the experimental group, when the control group is stored at the 24 th storage period, the MDA content of the experimental group is 21.6140nmol/g, the MDA content of the control group is 40.4955 nmol/g, which is 1.874 times that of the experimental group, and the MDA content of the experimental group is obviously lower than that of the control group (the MDA content of the experimental group is obviously lower than that of the control group)P<0.05 Indicating that the membrane lipid oxidation degree of the control group was high. In addition, the content of malondialdehyde can be used as an index for indirectly measuring the damage degree of plant cells, and the MDA content of an experimental group is always lower than that of a control group during the storage period, so that the low-temperature plasma sterilization technology treatment can effectively reduce the MDA accumulation of the picked flammulina velutipes, reduce the membrane lipid peroxidation degree of the picked flammulina velutipes, and better maintain the cell membrane integrity of the flammulina velutipes, thereby prolonging the storage time of the flammulina velutipes after being picked.
The content of soluble solids in Flammulina velutipes was measured according to "refractometer method for measuring content of soluble solids in fruits and vegetables" NY/T2637-2014, and the result is shown in FIG. 9. As can be seen from fig. 9, the content of soluble solids of the control group and the experimental group both decreased and then increased with the increase of the storage time, and the content of soluble solids of the experimental group was higher than that of the control group. The soluble solid is the main energy source of the respiration metabolism activity of the fruits and vegetables, the mass fraction of the soluble solid is an important index for reflecting the maturity and the storage quality of the fruits and vegetables, and the soluble solid is increased in the later storage period or caused by the decomposition of microorganisms and the metabolism influence of needle mushrooms. In 21 st to 24 th storage period, the soluble solid content of the experimental group is about 2 times of that of the control group, which shows that the soluble solid content change is obviously influenced in the later storage period after the low-temperature plasma treatment.
The vitamin C content of Flammulina velutipes (Fr.) Sing was determined according to the determination of ascorbic acid in GB 5009.86-2016 national food safety Standard, and the results are shown in FIG. 10. As can be seen from FIG. 10, the vitamin C content of the control group and the experimental group both showed a decreasing trend with the increase of the storage time, wherein the change rate of the vitamin C content of the control group is faster than that of the experimental group. The vitamin C content of the experimental group is significantly higher than that of the control group during the storage period of 6-12 days (P<0.05). The low-temperature plasma treatment can effectively delay the reduction of the vitamin C content of the picked flammulina velutipes and prolong the storage period of the flammulina velutipes.
The activity of Flammulina velutipes superoxide dismutase (SOD) was detected according to GB/T41906-2022 SOD activity detection method, and the results are shown in FIG. 11. As can be seen from fig. 11, the change in SOD activity of the control group and the experimental group both increased and then decreased with the increase in storage time. In the storage process, the SOD activity of the experimental group is obviously higher than that of the control group (P<0.05 And the SOD activity of the control group and the experimental group peaked at 6 d. The main function of superoxide dismutase (SOD) is to catalyze the change of superoxide anion in plant tissues into H 2 O 2 And O 2 Therefore, the SOD activity of the flammulina velutipes can be obviously induced to increase after the low-temperature plasma treatment, so that the oxidation resistance and the anti-aging capability of the flammulina velutipes are enhanced.
The activity of flammulina velutipes catalase CAT was detected according to GB/T5522-2008 test for catalase activity of grain and oil, and the results are shown in FIG. 12. As can be seen from FIG. 12, during the storage period from 0 d to 3d, the CAT activities of the control group and the experimental group both showed an increase trend, and appeared the first peak at 3d, at which time the CAT activity of the control group was 1.8825U/(g.min), the CAT activity of the experimental group was 2.6125U/(g.min), which was 1.388 times that of the control group, and when the storage period was 9d, the CAT activity of the control group and the experimental group appeared the second peak, at which time the CAT activity of the experimental group was 1.608 times that of the control group, and the CAT activity of the experimental group was significantly higher than that of the control group (CAT activity of the control group)P<0.05 But from)From the 15 th day of storage, the CAT activity of the control group is obviously higher than that of the experimental group, and at the 21 st day of storage, the maximum CAT activity value of the control group is 2.5813U/(g.min), which is 2.829 times that of the experimental group. Catalase (CAT) is capable of catalyzing H 2 O 2 Decomposition into H 2 O and O 2 Thereby effectively eliminating ROS. Therefore, the CAT activity of the flammulina velutipes can be obviously improved to a certain extent through the low-temperature plasma treatment, so that the oxidation resistance of the flammulina velutipes is improved.
The activity of Flammulina velutipes peroxidase POD was detected according to GB/T32131-2015 colorimetric method for detecting horseradish peroxidase activity, and the results are shown in FIG. 13. As can be seen from fig. 13, the POD activity change of the control group shows a trend of first decreasing slowly and then increasing, while the POD activity of the control group is higher than that of the experimental group at storage 15d and 18d, the PDD activity of the experimental group reaches a first peak at 9d, which is 641.97U/g, which is 3.6 times that of the control group, and the POD activity of the experimental group shows an increasing trend at storage 15d to 24d, wherein the POD activity of the experimental group is significantly higher than that of the control group at storage 6d, 9d, 12d, 21d and 24dP<0.05 And shows that the POD activity of the flammulina velutipes can be improved to a certain extent after low-temperature plasma treatment. Peroxidase (POD) can remove free radicals, so that damage of the free radicals to cell membranes is reduced, and the purpose of delaying senescence of tissue cells is achieved, and thus low-temperature plasma treatment can delay senescence and deterioration of needle mushrooms.
The change of the free radical DPPH of the flammulina velutipes is detected according to the polypeptide oxidation resistance determination DPPH and ABTS method GB/T39100-2020, and the result is shown in FIG. 14. As can be seen from fig. 14, the DPPH clearance of the control group was increased during the storage period from 0 to 6d, and reached the first peak at 6d, which was 41% and was 3.417 times that of the experimental group, while the DPPH clearance of the experimental group was decreased during this period, and reached the first peak at 9d, which was 20.66%. The DPPH clearance rate of the experimental group is significantly higher than that of the control group during the storage period of 12-24 d: (P<0.05 And the DPPH clearance changes in the control and experimental groups appeared to be increasing from storage 18 d). The DPPH clearance rate indicates the antioxidant activity of the sample liquid within a certain range, so that the low-temperature plasma technology is known to treat the sample liquid in storageThe DPPH clearance rate of the needle mushrooms can be effectively improved in the middle and later stages of storage, so that the antioxidant activity of the needle mushrooms is improved.
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (2)
1. A low-temperature plasma processing method suitable for postharvest preservation of needle mushrooms is characterized by comprising the following steps:
the method comprises the following steps: selecting needle mushrooms which are complete in mushroom body, white in color, free of opening, diseases, insect pests and mechanical injuries, putting the needle mushrooms into a polypropylene box, and hermetically packaging the needle mushrooms by adopting a polyethylene plastic film;
step two: placing the sealed needle mushrooms in a treatment bin of a DBD-CPCS device, wherein the treatment conditions are as follows: the temperature is 4 ℃, the relative humidity is 90%, the distance between the polar plates is 29cm, the processing frequency is 60-150Hz, the processing voltage is 55-105kV, and the processing time is 60-210s.
2. A low-temperature plasma processing method suitable for postharvest preservation of needle mushrooms is characterized by comprising the following steps:
the method comprises the following steps: selecting needle mushrooms which are complete in mushroom body, white in color, free of opening, diseases, insect pests and mechanical injuries, putting the needle mushrooms into a polypropylene box, and hermetically packaging the needle mushrooms by adopting a polyethylene plastic film;
step two: placing the sealed needle mushrooms in a treatment bin of a DBD-CPCS device, wherein the treatment conditions are as follows: the temperature is 4 ℃, the relative humidity is 90%, the distance between the polar plates is 29cm, the processing frequency is 150Hz, the processing voltage is 95kV, and the processing time is 150s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211537978.2A CN115769833A (en) | 2022-12-01 | 2022-12-01 | Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211537978.2A CN115769833A (en) | 2022-12-01 | 2022-12-01 | Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115769833A true CN115769833A (en) | 2023-03-10 |
Family
ID=85391138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211537978.2A Pending CN115769833A (en) | 2022-12-01 | 2022-12-01 | Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115769833A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145449A (en) * | 2021-09-25 | 2022-03-08 | 福建省青楚堂健康管理有限公司 | Pretreatment technology before edible mushroom processing |
-
2022
- 2022-12-01 CN CN202211537978.2A patent/CN115769833A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145449A (en) * | 2021-09-25 | 2022-03-08 | 福建省青楚堂健康管理有限公司 | Pretreatment technology before edible mushroom processing |
Non-Patent Citations (2)
| Title |
|---|
| L AGUN，ET AL.: "The Efficiency of DBD Cold Plasma Pen Treatment on the Oyster Mushroom Bacterial Decontamination", JOURNAL OF PHYSICS: CONFERENCE SERIES, pages 1 - 5 * |
| NGUYEN PHUOC MINH: "Corona discharge power of plasma treatment influence on the physicochemical and microbial quality of enoki mushroom (Flammulinavelutipes)", J PURE APPL MICROBIOL, vol. 16, no. 1, pages 1 - 11 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Belay et al. | Influence of initial gas modification on physicochemical quality attributes and molecular changes in fresh and fresh-cut fruit during modified atmosphere packaging | |
| Lin et al. | Research advances in browning of button mushroom (Agaricus bisporus): Affecting factors and controlling methods | |
| Pan et al. | Inhibition of fruit softening by cold plasma treatments: affecting factors and applications | |
| Ji et al. | Effect of atmospheric cold plasma treatment on antioxidant activities and reactive oxygen species production in postharvest blueberries during storage | |
| Pinto et al. | Effect of modified atmosphere packaging (MAP) and gaseous ozone pre-packaging treatment on the physico-chemical, microbiological and sensory quality of small berry fruit | |
| Hu et al. | Potential use of atmospheric cold plasma for postharvest preservation of blueberries | |
| Mahunu et al. | Improving the biocontrol efficacy of Pichia caribbica with phytic acid against postharvest blue mold and natural decay in apples | |
| CN104126645B (en) | Method for increasing nutritive value of blueberries during storage period | |
| Rocha et al. | Shelf-life of chilled cut orange determined by sensory quality | |
| Xiao et al. | Extension of the shelf-life of fresh oyster mushrooms (Pleurotus ostreatus) by modified atmosphere packaging with chemical treatments | |
| CN103548986A (en) | Refreshing method of edible fungi | |
| WO2023221378A1 (en) | High-oxygen stress freshness preservation method suitable for fresh fruit storage, and application | |
| Liu et al. | Effect of ozone treatment on the quality and enzyme activity of Lentinus edodes during cold storage | |
| Jiang | Effect of natamycin in combination with pure oxygen treatment on postharvest quality and selected enzyme activities of button mushroom (Agaricus bisporus) | |
| Zhang et al. | High-voltage electrostatic field-assisted modified atmosphere packaging for long-term storage of pakchoi and avoidance of off-flavors | |
| Tian et al. | Loquat (Eriobotrya japonica L.) | |
| Xie et al. | Comparison of sodium acid sulfate and UV‐C treatment on browning and storage quality of fresh‐cut potatoes | |
| CN114903079B (en) | Fresh-keeping method for delaying color change and lignification of fresh velvet mushroom fruiting bodies | |
| CN115769833A (en) | Low-temperature plasma treatment method suitable for postharvest preservation of flammulina velutipes | |
| Jiang et al. | L-Cysteine Treatment Delayed the Quality Deterioration of Fresh-Cut Button Mushrooms by Regulating Oxygen Metabolism, Inhibiting Water Loss, and Stimulating Endogenous H2S Production | |
| Galgano et al. | Effects of anti-UV film and protective atmosphere on fresh-cut iceberg lettuce preservation | |
| CN214758919U (en) | LED box cover equipment for fresh-keeping and ripening of banana fruits | |
| CN108142546A (en) | A kind of preservation method of broccoli and application | |
| Chandran | Effect of film packaging in extending shelf life of dragon fruit, Hylocereus undatus and Hylocereus polyrhizus | |
| Li et al. | Research on energy metabolism, lignification and veil opening in postharvest white mushroom (Agaricus bisporus) under high O 2/CO 2 controlled atmospheres (HOC-CA). |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |