CN115747180A - Enzyme composition and application thereof - Google Patents
Enzyme composition and application thereof Download PDFInfo
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- CN115747180A CN115747180A CN202211388414.7A CN202211388414A CN115747180A CN 115747180 A CN115747180 A CN 115747180A CN 202211388414 A CN202211388414 A CN 202211388414A CN 115747180 A CN115747180 A CN 115747180A
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- glucose oxidase
- glucanase
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses an enzyme composition and application thereof, belonging to the technical field of microorganisms. The enzyme composition consists of beta-1, 3-glucanase and glucose oxidase; wherein the mass ratio of the beta-1, 3-glucanase to the glucose oxidase is 5-80. The invention establishes a multi-enzyme linked cascade reaction system by jointly using beta-1, 3-glucanase and glucose oxidase, and destroys the biomembrane matrix by degrading matrix components, especially polysaccharide components, thereby having remarkable removing capability to the candida albicans biofilm; meanwhile, the glucose oxidase can also generate hydrogen peroxide, and has a killing effect on candida albicans inside the biofilm. On the basis, the invention provides a method for removing the candida albicans biofilm, which has high removal rate on the candida albicans biofilm, good sterilization effect and better application prospect.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to an enzyme composition and application thereof.
Background
Candida albicans is a common fungal pathogen, which can parasitize the skin and mucous membranes of the human body, and when the immunity of the body is low or affected by certain factors, the Candida albicans can cause fungal infections of different parts to different degrees. Candida albicans forms a broad range of biofilms on mucosal surfaces during infection. Biofilms are microbial aggregates formed by the adhesion of fungal cells to contacting surfaces, the production of large amounts of extracellular matrix (primarily polysaccharides) by themselves, surrounding themselves. The extracellular matrix not only participates in the formation of a biofilm structure, but also protects internal microorganisms from the influence of the external severe environment, and obviously improves the drug resistance of the microorganisms.
Disclosure of Invention
The invention provides an enzyme composition, which consists of beta-1, 3-glucanase and glucose oxidase; wherein the mass ratio of the beta-1, 3-glucanase to the glucose oxidase is 5-80. Preferably, the mass ratio of the beta-1, 3-glucanase to the glucose oxidase is 10.
The invention provides application of the enzyme composition in removing candida albicans biofilm.
The invention also provides application of the enzyme composition in preparation of a preparation for removing candida albicans biofilm. Preferably, the preparation is selected from mixed enzyme solutions of the enzyme composition; in the mixed enzyme solution, the solvent includes, but is not limited to, water, PBS buffer solution, and the like, which can disperse or dissolve the beta-1, 3-glucanase and the glucose oxidase therein without inhibiting the enzyme activity. In the mixed enzyme solution, the concentration of the beta-1, 3-glucanase is preferably 5-80 mug/mL, and the concentration of the glucose oxidase is preferably 30-500 mug/mL; preferably, the concentration of the beta-1, 3-glucanase is preferably 10. Mu.g/mL, and the concentration of the glucose oxidase is preferably 250. Mu.g/mL.
The invention provides an enzyme preparation, which is a mixed enzyme solution formed by dispersing or dissolving beta-1, 3-glucanase and glucose oxidase in a solvent, wherein the concentration of the beta-1, 3-glucanase is preferably 5-80 mug/mL, and the concentration of the glucose oxidase is preferably 30-500 mug/mL; the solvent includes, but is not limited to, water, PBS buffer, and the like, which can disperse or dissolve the β -1, 3-glucanase and the glucose oxidase therein without inhibiting the enzyme activity. Preferably, the concentration of the beta-1, 3-glucanase is preferably 10. Mu.g/mL, and the concentration of the glucose oxidase is preferably 250. Mu.g/mL.
The enzyme composition and the enzyme preparation can be used for killing candida albicans, and can also be used for assisting other active ingredients to kill the candida albicans. Therefore, the invention provides the application of the enzyme composition and/or the enzyme preparation in inhibiting candida albicans; the application may be diagnostic or non-diagnostic.
The invention provides a candida albicans biofilm removing method, which comprises the following steps:
the Candida albicans and the biofilm thereof are treated by the mixed enzyme solution, the treatment temperature is 30-37 ℃, and the treatment time is 12-24 h, so as to achieve the purpose of removing the biofilm.
The treatment temperature is preferably 30 ℃.
The treatment time is preferably 24 hours.
The above treatment method may be a treatment method in which the mixed enzyme solution is sufficiently contacted with the Candida albicans biofilm, such as coating or spraying.
In the invention, the beta-1, 3-glucanase is proved to have the effect of assisting the glucose oxidase to improve the yield of the hydrogen peroxide, and therefore, the invention provides the application of the beta-1, 3-glucanase in assisting the glucose oxidase to improve the yield of the hydrogen peroxide.
The invention also provides application of the enzyme composition and/or the enzyme preparation in improving the yield of the hydrogen peroxide.
Preferably, the enzyme composition and/or enzyme preparation can improve the hydrogen peroxide yield in a bacteriostatic system during the inhibition of candida albicans. In the enzyme composition and/or the enzyme preparation, the concentration of the beta-1, 3-glucanase is selected from 10 mu g/mL, and the concentration of the glucose oxidase is selected from 250 mu g/mL.
The invention has the beneficial effects that:
the invention establishes a multi-enzyme linked cascade reaction system by jointly using beta-1, 3-glucanase and glucose oxidase, and destroys the biomembrane matrix by degrading matrix components, especially polysaccharide components, thereby having remarkable removing capability to the candida albicans biofilm. Meanwhile, the glucose oxidase can also generate hydrogen peroxide, and has a remarkable killing effect on candida albicans inside the biological envelope. In addition, the anti-biofilm strategy of the invention can overcome the limitation of single enzyme, improve the antibacterial efficiency and is not easy to induce bacterial drug resistance.
Drawings
FIG. 1 shows the bacteriostatic effect of a single enzyme; wherein, A picture is beta-1, 3-glucanase with different concentrations; b is graph of glucose oxidase with different concentrations;
FIG. 2 is a scanning electron micrograph of Candida albicans and its biofilm; wherein, A is blank control; panel B is 10. Mu.g/mL beta-1, 3-glucanase; panel C is 250 μ g/mL glucose oxidase; panel D is 10. Mu.g/mL beta-1, 3-glucanase + 250. Mu.g/mL glucose oxidase;
FIG. 3 is a fluorescence micrograph of live/dead Candida albicans cells in the biofilm; wherein, A is blank control group; panel B is 10. Mu.g/mL beta-1, 3-glucanase; panel C is 250 μ g/mL glucose oxidase; panel D is 10. Mu.g/mL beta-1, 3-glucanase + 250. Mu.g/mL glucose oxidase.
Detailed Description
Other terms used in the present invention have generally the meanings that are commonly understood by those of ordinary skill in the art, unless otherwise specified. The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention, but not to limit the scope of the invention in any way.
Example 1
And (3) testing the single-enzyme antibacterial effect:
PBS is used as a solvent to prepare a beta-1, 3-glucanase solution, and concentration gradients are set to be 15.6 mu g/mL, 31.2 mu g/mL, 62.5 mu g/mL, 125 mu g/mL, 250 mu g/mL, 500 mu g/mL and 1000 mu g/mL respectively. Glucose oxidase solution was prepared using PBS as a solvent, and concentration gradients were set at 7.8. Mu.g/mL, 15.6. Mu.g/mL, 31.2. Mu.g/mL, 62.5. Mu.g/mL, 125. Mu.g/mL, 250. Mu.g/mL, 500. Mu.g/mL, and 1000. Mu.g/mL, respectively.
Respectively weighing 100 μ L of the above extractBeta-1, 3-glucanase solution, glucose oxidase solution, and Candida albicans suspension (10) 6 CFU/mL) were added to 96-well plates and incubated at 30 ℃ for 24h. The turbidity degree of the bacteria liquid in the 96-well plate is observed, and the MIC value of the single enzyme is determined.
The test results are shown in fig. 1:
the wells treated with the beta-1, 3-glucanase were all in a turbid state, indicating that the beta-1, 3-glucanase had no bacteriostatic effect. Whereas wells treated with 125. Mu.g/mL, 250. Mu.g/mL, 500. Mu.g/mL, 1000. Mu.g/mL glucose oxidase were clear and transparent, and therefore the MIC value of glucose oxidase was 125. Mu.g/mL.
Example 2
Preparation of candida albicans biofilm:
candida albicans was activated and cultured, and then diluted with a medium so that the A value thereof was 0.01 at 600nm in a spectrophotometer. 100 μ L of the bacterial solution was added to a 96-well plate, and incubated at 30 ℃ for 48 hours to form a biofilm.
Example 3
Removal of candida albicans biofilm:
the following groups are set: (1) blank control; (2) 5 mu g/mL beta-1, 3-glucanase solution; (3) 10 mu g/mL beta-1, 3-glucanase solution; (4) 125. Mu.g/mL glucose oxidase solution; (5) 250 mug/mL glucose oxidase solution; (6) mixing enzyme solution: 5. Mu.g/mL beta-1, 3-glucanase + 125. Mu.g/mL glucose oxidase; (7) mixing enzyme solution: 5. Mu.g/mL beta-1, 3-glucanase + 250. Mu.g/mL glucose oxidase; (8) mixing enzyme solution: 10. Mu.g/mL beta-1, 3-glucanase + 125. Mu.g/mL glucose oxidase; (9) mixing enzyme solution: 10. Mu.g/mL beta-1, 3-glucanase + 250. Mu.g/mL glucose oxidase.
Candida albicans biofilm prepared in example 2 was washed 3 times with PBS and divided into eight groups, and 100. Mu.L of the above enzyme solutions were added, respectively, and cultured at 30 ℃ for 24 hours.
The clearance of the biofilm was measured by MTT staining. The treated Candida albicans biofilm was washed 3 times with PBS and stained with 10. Mu.L MTT solution (5 mg/mL) + 90. Mu.L sterile PBS for 4h. The supernatant was aspirated and the cells were treated with 110. Mu.L DMSO. Measuring the A content of the extractive solution with enzyme-labeling instrument 490 Value to determineBiofilm content.
The test results are shown in table 1:
TABLE 1
| Group of | (1) | (2) | (3) | (4) | (5) | (6) | (7) | (8) | (9) |
| Biofilm content (%) | 100 | 91.09 | 82.25 | 49.45 | 35.96 | 28.93 | 23.15 | 23.77 | 19.24 |
As can be seen from Table 1, compared with the single beta-1, 3-glucanase and glucose oxidase, the mixed enzyme solution has very remarkable effect of removing the Candida albicans biofilm, wherein the clearance rate of 10 mug/mL beta-1, 3-glucanase and 250 mug/mL glucose oxidase is the highest and can reach about 80%.
Example 4
Biofilm structural changes:
1mL of Candida albicans suspension (10) 6 CFU/mL) and 1mL YPD medium were added to a 24-well plate, and the chip was placed in the bottom of the 24-well plate and cultured for 48h to form a biofilm. The supernatant was carefully removed and washed 3 times with PBS. 10. Mu.g/mL of beta-1, 3-glucanase, 250. Mu.g/mL of glucose oxidase, and 1mL of each of 10. Mu.g/mL of beta-1, 3-glucanase and 250. Mu.g/mL of glucose oxidase were added to the formed biofilm, followed by incubation for 24 hours. Wash 3 times with PBS. And then placing the chip in 2.5% glutaraldehyde solution for fixing for 4h, then placing the chip in 30% ethanol, 50% ethanol, 70% ethanol, 80% ethanol, 90% ethanol and 100% ethanol in sequence for dehydration and replacing the dehydrated chip with 100% tert-butyl alcohol, taking out the chip, freeze-drying the chip for 48h, spraying gold, and observing the influence of the enzyme solution on the structural change of the candida albicans biofilm by adopting a 10kv Scanning Electron Microscope (SEM).
The test results are shown in fig. 2:
as can be seen from FIG. 2, candida albicans in the control group formed a dense three-dimensional structure of the biofilm (Panel A); biofilm structure was looser in comparison to the blank control after treatment with β -1, 3-glucanase alone (panel B). After being treated by glucose oxidase alone, the bacteria content is obviously reduced compared with a blank control group, which indicates that the glucose oxidase has a certain bactericidal effect but still has a bacterial cluster (figure C). However, the Candida albicans biofilm structure was disrupted by the mixed enzyme treatment, and cell detachment was more severe (panel D).
Example 5
And (3) observation by a fluorescence microscope:
1mL of Candida albicans suspension (10) 6 CFU/mL) and 1mL YPD medium were addedThe biofilm was formed in 24-well plates for 48 h. The supernatant was carefully removed and washed 3 times with PBS. 10. Mu.g/mL of beta-1, 3-glucanase, 250. Mu.g/mL of glucose oxidase, and 1mL of each of 10. Mu.g/mL of beta-1, 3-glucanase and 250. Mu.g/mL of glucose oxidase were added to the formed biofilm, followed by incubation for 24 hours. Wash 3 times with PBS. By using
BacLight staining TM bacterial viability and enumeration kit treatment. 1 volume of DMAO and 2 volumes of EthD-III were mixed in a microcentrifuge tube, and then 8 volumes of 0.85% sodium chloride solution were added to give a 100 Xdye solution. Then incubated in the dark at room temperature for 15min. Biofilms were visualized using a fluorescence microscope with FITC and Cy3 channels. When active/dead
When the BacLight bacterial viability and enumeration kit stains candida albicans, live cells show green fluorescence, damaged or dead cells show yellow green or red fluorescence.
The test results are shown in fig. 3:
as can be seen from FIG. 3, almost all of the fluorescence of untreated Candida albicans was green fluorescence, and was large and dense (Panel A). Candida albicans treated with the beta-1, 3-glucanase was also almost green, but had a more relaxed structure than the blank (panel B). After glucose oxidase treatment, red and yellow fluorescence occupy most of the graph, which indicates that glucose oxidase has killing capacity on candida albicans, but yellow fluorescence has large area and compact structure, which indicates that glucose oxidase can kill candida albicans on the surface, but the biofilm can still protect the internal candida albicans from killing (graph C). After the mixed enzyme treatment, the red and yellow fluorescence is very strong, accompanied by phenomena of bacterial sparseness, dispersion and the like, which indicates that the structure of the biofilm is destroyed and candida albicans is largely killed (figure D). The above results indicate that mixed enzyme treatment more readily cleaves biofilms and kills candida albicans cells harboring in biofilms.
Example 6
Hydrogen peroxide yield impact:
100 μ L of Candida albicans suspension (10) 6 CFU/mL) and 100. Mu.L YPD medium were added to a 96-well plate and cultured for 48h to form a biofilm. The supernatant was carefully removed and washed 3 times with PBS. The formed biofilm was added with 100. Mu.L each of 10. Mu.g/mL of beta-1, 3-glucanase, 250. Mu.g/mL of glucose oxidase, 10. Mu.g/mL of beta-1, 3-glucanase + 250. Mu.g/mL of glucose oxidase, and cultured for 24 hours. Wash 3 times with PBS. The hydrogen peroxide content in each culture system was determined using a hydrogen peroxide kit.
The measurement results are shown in table 2:
TABLE 2
| Group of | H 2 O 2 (μmol/mL) |
| Blank control | 0.0626 |
| 10. Mu.g/mL beta-1, 3-glucanase | 0.0534 |
| 250 mug/mL glucose oxidase | 0.1855 |
| 10. Mu.g/mL beta-1, 3- |
0.5613 |
As can be seen from Table 2, the culture system treated with glucose oxidase produced more hydrogen peroxide than the blank control, while the culture system treated with β -1, 3-glucanase did not actually improve the production of hydrogen peroxide. However, after the mixed enzyme treatment, the hydrogen peroxide content in the culture system can be obviously improved on the basis of the treatment of the glucose oxidase alone. This suggests that the beta-1, 3-glucanase can assist glucose oxidase in increasing the production of hydrogen peroxide.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (10)
1. An enzyme composition consisting of a beta-1, 3-glucanase and a glucose oxidase; wherein the mass ratio of the beta-1, 3-glucanase to the glucose oxidase is 5-80; preferably, the mass ratio of the beta-1, 3-glucanase to the glucose oxidase is 10.
2. Use of the enzyme composition according to claim 1 for the removal of candida albicans biofilm.
3. Use of the enzyme composition according to claim 1 for the preparation of a preparation for removing candida albicans biofilm.
4. An enzyme preparation comprising a mixed enzyme solution obtained by dispersing or dissolving the enzyme composition according to claim 1 in a solvent; wherein the concentration of the beta-1, 3-glucanase is 5-80 mug/mL, and the concentration of the glucose oxidase is 30-500 mug/mL.
5. The enzyme preparation according to claim 4, wherein the concentration of the beta-1, 3-glucanase is 10 μ g/mL and the concentration of the glucose oxidase is 250 μ g/mL.
6. Use of the enzyme composition according to claim 1 and/or the enzyme preparation according to claim 4 for the inhibition of candida albicans for non-therapeutic purposes.
7. A method for removing Candida albicans biofilm is characterized by comprising the following steps:
candida albicans and its biofilm were treated with the enzyme preparation according to claim 4 at 30-37 ℃ for 12-24 hours for biofilm removal.
8. Application of beta-1, 3-glucanase in assisting glucose oxidase to improve hydrogen peroxide yield.
9. Use of the enzyme composition according to claim 1 and/or the enzyme preparation according to claim 4 for increasing the production of hydrogen peroxide.
10. Use according to claim 9, wherein the enzyme composition and/or enzyme preparation is used for increasing the production of hydrogen peroxide in bacteriostatic systems during the inhibition of candida albicans.
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Patent Citations (6)
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| WO2022079315A1 (en) * | 2020-10-16 | 2022-04-21 | Onelife S.A. | Parapharmaceutical or pharmaceutical composition administrable to a living being, preferably a human being, comprising at least one enzyme for the treatment and/or prevention of bacterial infections involving biofilm formation |
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