CN115737904A - Skin repairing film capable of degrading and generating breathing holes and preparation method thereof - Google Patents
Skin repairing film capable of degrading and generating breathing holes and preparation method thereof Download PDFInfo
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Abstract
A skin repairing membrane capable of degrading and generating breathing holes and a preparation method thereof, the skin repairing membrane is of a sponge structure, raw materials of the skin repairing membrane comprise a material liquid 1, a material liquid 2 and an ionic cross-linking agent, the skin repairing membrane comprises a skin simulation structure and a plurality of degradation column structures supported inside the skin simulation structure, the skin simulation structure is made of the material liquid 1 and the ionic cross-linking agent, the degradation column structures are made of the material liquid 2, wherein the material liquid 1 is made of collagen, gelatin and/or other high polymer materials; the feed liquid 2 is prepared from polyethylene glycol. The skin repairing film capable of degrading to generate the breathing pores is of a compact sponge structure, and is flexible and elastic. The skin repairing film capable of degrading to generate the breathing holes has the advantages of stable structure, smooth and fine sample surface, strong liquid absorption capacity, relatively simple and convenient manufacturing process, high repeatability and high product safety, and can be widely applied to the field of medical appliances. The product degrades after being placed at the wound site for 30d, which can lead the regeneration of skin tissue.
Description
Technical Field
The invention belongs to the technical field of biomedical materials, and particularly relates to a skin repairing film capable of degrading to generate a breathing hole and a preparation method thereof.
Background
The largest tissue organ of the human body is the skin, which provides a physical barrier to the surface of the human body and prevents most of the harmful substances from entering the human body. Meanwhile, the skin is attached to a series of tissues such as hair, sweat glands, sebaceous glands and the like, so that the skin also has a series of functions of excreting and secreting hormones and the like. Formally, based on the complexity of the above functions, skin repair has been a difficult problem in the field of human tissue repair. Particularly for severe damage repair of damage to the dermis, no good solution exists at present.
The reported patents or products at home and abroad claim to have better repairing effect, but the actual clinical effect is limited. The Chinese patent with publication number CN 111840633A mentions a skin repairing film and a preparation method thereof, the skin repairing film is prepared by adopting carboxymethyl cellulose and bioactive glass, wherein the bioactive glass is directly mixed into a system and cannot provide powerful mechanical support.
Disclosure of Invention
The technical problem to be solved is as follows: aiming at the technical problems, the invention provides the skin repairing film capable of degrading and generating the breathing holes and the preparation method thereof, and the prepared skin repairing film capable of degrading and generating the breathing holes has a simple structure and is convenient to prepare; when in use, the material has the characteristics of uniform pores, fine and smooth surface and the like, and the material has air holes in due time along with the prolonging of the standing time, thereby helping the regeneration of skin tissues.
The technical scheme is as follows: a skin repairing film capable of degrading and generating a breathing hole is of a sponge structure, raw materials of the skin repairing film comprise a material liquid 1, a material liquid 2 and an ionic cross-linking agent, the skin repairing film comprises a skin simulation structure and a plurality of degradation column structures supported inside the skin simulation structure, the skin simulation structure is made of the material liquid 1 and the ionic cross-linking agent, the degradation column structures are made of the material liquid 2, wherein the material liquid 1 is made of collagen, gelatin and/or other high polymer materials and water; the feed liquid 2 is prepared from polyethylene glycol and water, the mass ratio of the feed liquid 1 to the feed liquid 2 to the ionic crosslinking agent is (1-10) to (0.1-0.2), and the ratio of collagen, gelatin and/or other high molecular materials to water in the feed liquid 1 is 1-6g:94-99ml, the mass ratio of polyethylene glycol to water in the feed liquid 2 is 2-10:90 to 98.
Preferably, the collagen is at least one of bone collagen and skin collagen, the gelatin is at least one of bone gelatin and skin gelatin, and the other polymer material is at least one of sodium carboxymethylcellulose, croscarmellose sodium, polylactic acid, hyaluronic acid, carboxymethyl chitosan, sodium alginate and hyaluronic acid.
Preferably, the polyethylene glycol is at least one of PEG-200, PEG-400, PEG-2000 and PEG-6000.
Preferably, said degradable respiratory pore producing skin repair membrane is of type a, type B, type C and type D, wherein type a has a thickness of 0.1 to 0.5mm, a dimension of 100 x 200mm, 100 x 300mm or 300 x 300mm, type B has a thickness of 0.5 to 0.8mm, a dimension of 50 x 200mm or 100 x 300mm, type C has a thickness of 0.8 to 1.2mm, a dimension of 100 x 100mm or 100 x 200mm, type D has a thickness of 1.2 to 2mm, a dimension of 100 x 300mm, 300 x 300 or 400.
Based on the preparation method of the skin repairing film capable of degrading and generating the breathing hole, the steps are as follows:
dissolving a feed liquid 1, adding an ionic cross-linking agent for cross-linking treatment, reacting for 4 hours at 40-90 ℃, then pouring the solution into a stainless steel container with a stand column (space occupying column), then placing the stainless steel container into a liquid nitrogen tank, standing for 1-8 hours until the stainless steel container is frozen and frozen, and then taking out for later use;
step two, putting the feed liquid 2 into a water bath at the temperature of 60-90 ℃, stirring for 1-9 hours for dissolving, pouring the dissolved feed liquid 2 into holes, taking out the space occupying columns in the frozen ice prepared in the step one during pouring, putting the whole into liquid nitrogen after pouring is finished, and standing;
step three, drying the frozen product processed in the step two;
and step four, sterilizing the dried reaction product to obtain a finished product.
Preferably, the drying mode in the third step is freeze vacuum drying, vacuum drying or ethanol dehydration.
Preferably, the vacuum freeze-drying in the third step comprises the following specific steps:
a) Freezing under vacuum at-40-55 deg.C for 1-3 h;
b) Continuously freezing under vacuum at the temperature of-10 to-15 ℃ for 2 to 10 hours;
c) Continuously freezing under vacuum at 0-5 deg.C for 12-24 hr;
d) Continuously freezing under vacuum at 15-25 deg.C for 1-5h, and completely lyophilizing;
the vacuum drying method is that under the vacuum condition, the temperature is controlled at 20 to 30 ℃, and the vacuum drying is carried out for 20 to 48 hours;
the method for dehydrating the ethanol comprises the steps of putting a product into 75-100wt% ethanol, putting the product into an ultrasonic cleaning machine, performing ultrasonic treatment for 1-5h, pouring out the ethanol after the ultrasonic treatment is finished, repeating the step for 1-3 times until the product is softened, then putting the treated product into a forced air drying oven, setting the temperature to be 20-50 ℃, and drying for 10-24h to remove the ethanol.
Preferably, the mode of the sterilization treatment in the fourth step is at least one of cobalt-60 irradiation sterilization, electron beam irradiation sterilization and ethylene oxide sterilization.
Based on the application method of the skin repair film capable of degrading and generating the breathing holes, the method comprises the following steps: the skin repairing film capable of degrading to generate the breathing holes is applied to the skin wound of a patient after being wholly or cut, if necessary, normal saline can be properly dripped to wash the wound, then the wound is fixed by using a medical adhesive tape to prevent slipping, after 30 days, the medical adhesive tape is taken down, regularly arranged pores can be seen on the surface of the repairing film, the pores can be beneficial to repairing and regenerating skin tissues, when the product is placed for about 30 days, the whole structure is disintegrated, at the moment, the material is washed clean by using the normal saline, and the skin can be well repaired.
Has the advantages that: 1. the invention discloses a method for forming a sample by adopting a step-by-step freeze-drying method to obtain a uniform porous material with a special spatial structure. Meanwhile, the material has the characteristics of high elasticity, high water absorption and prolonged degradation time by adopting an ionic crosslinking mode. Meanwhile, compared with the traditional chemical crosslinking, the ionic crosslinking mode is more efficient and higher in safety.
2. The skin membrane prepared by the invention has higher toughness and stronger water absorption capacity, and the water absorption capacity is 5-30 times of the self weight.
Drawings
FIG. 1 is a schematic view of a skin repairing film structure capable of degrading to generate a breathing hole according to the present invention;
FIG. 2 is a schematic structural view of a skin repair film forming container capable of degrading to generate a breathing hole according to the present invention;
the numerical designations in the drawings represent the following: 1. a skin-mimicking structure; 2. degrading the pillar structure; 3. a movable occupation column; 4. stainless steel molds.
Detailed Description
A skin repairing film capable of degrading and generating a breathing hole is of a sponge structure, raw materials of the skin repairing film comprise a material liquid 1, a material liquid 2 and an ionic cross-linking agent, the skin repairing film comprises a skin simulation structure and a plurality of degradation column structures supported inside the skin simulation structure, the skin simulation structure is made of the material liquid 1 and the ionic cross-linking agent, the degradation column structures are made of the material liquid 2, wherein the material liquid 1 is made of collagen, gelatin and/or other high polymer materials and water; the feed liquid 2 is prepared from polyethylene glycol and water, the mass ratio of the feed liquid 1 to the feed liquid 2 to the ionic crosslinking agent is (1-10) to (0.1-0.2), and the ratio of collagen, gelatin and/or other high molecular materials to water in the feed liquid 1 is 1-6g:94-99ml, the mass ratio of polyethylene glycol to water in the feed liquid 2 is 2-10:90-98.
The collagen is at least one of ossein and collagen skin, the gelatin is at least one of ossein and collagen skin, and the other high polymer material is at least one of sodium carboxymethylcellulose, croscarmellose sodium, polylactic acid, hyaluronic acid, carboxymethyl chitosan, sodium alginate and hyaluronic acid.
The polyethylene glycol is at least one of PEG-200, PEG-400, PEG-2000 and PEG-6000.
The skin repair membrane capable of degrading to produce the respiratory pores is divided into an A type, a B type, a C type and a D type, wherein the thickness of the A type is 0.1-0.5mm, the size is 100 x 200mm, 100 x 300mm or 300 x 300mm, the thickness of the B type is 0.5-0.8mm, the size is 50 x 200mm or 100 x 300mm, the thickness of the C type is 0.8-1.2mm, the size is 100 x 100mm or 100 x 200mm, the thickness of the D type is 1.2-2mm, and the size is 100 x 300mm, 300 or 400 x 400.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
The invention will be further described with reference to the drawings and preferred embodiments, but the invention is not limited to the following embodiments.
Example 1
The embodiment provides a preparation method of a skin repairing film capable of degrading and generating a breathing hole, which comprises the following steps:
1) 2g of collagen powder was added to a beaker, 98ml of purified water was added thereto to swell the collagen powder for 3 hours, the mixture was heated to 40 ℃ and 1g of sodium carboxymethylcellulose was slowly added thereto under stirring.
2) After the solution in the step 1) is dissolved, adding 1g of ionic crosslinking agent calcium sulfate, and reacting for 4 hours at 40 ℃. Then pouring the mixture into a forming container (container structure diagram, see figure 2) with occupation holes, wherein the movable occupation columns 3 in the stainless steel mold 4 in the container are distributed in 5 rows and 7 columns, the diameter of each movable occupation column 3 is 2mm, and the height of each movable occupation column is 20mm. Putting the container into a liquid nitrogen tank, standing for 1 hour, and taking out the container for later use after the solution is completely frozen; the process can obtain even and compact prefreezing structure of the porous skin repairing film.
3) After the step 2 is finished, dissolving polyethylene glycol, preparing 2wt% of polyethylene glycol-200 solution, putting the solution into a water bath at 60 ℃, stirring for 9 hours, and dissolving for later use.
4) And (3) after the step 3 is finished, pouring the product onto the frozen body in the step 2, taking out the occupying columns of the freeze-drying mold when pouring is noticed, and then pouring the solution obtained in the step 3 into the holes. And (4) after the solution in the step (3) is filled, putting the whole system into a liquid nitrogen tank, and standing for 12 hours.
5) After the step 4 is finished, freeze-drying the frozen product, wherein the temperature of a partition plate of a freeze dryer is-40 ℃ for 3 hours according to a); b) The temperature of a clapboard of the freeze dryer is-15 ℃ and the time is 8 hours; c) The temperature of a clapboard of the freeze dryer is 0 ℃ and the time is 24 hours; d) The temperature of a clapboard of the freeze dryer is 15 ℃, and the time is 4 hours; through the freeze-drying process, the product can be completely freeze-dried.
6) And (5) after the step 5 is finished, putting the reaction product into a bubble cap and an aluminum-plastic bag, and performing sterilization treatment to obtain a finished product. Structure diagram of the final product fig. 1 is shown, wherein 1 is a skin-simulated structure, and 2 is a degradation column structure.
The sterilization mode mentioned in step 6 is cobalt-60 irradiation sterilization, and the irradiation dose is 25k.
Example 2
The embodiment provides a preparation method of a skin repairing film capable of degrading and generating a breathing hole, which comprises the following steps:
1) 2g of gelatin powder and 98ml of purified water were added to a beaker, and after swelling for 14 hours, the mixture was heated to 50 ℃ and 1g of carboxymethyl chitosan was slowly added thereto with stirring.
2) After the solution in the step 1) is dissolved, 1g of calcium chloride is added, and the reaction is carried out for 4 hours at 60 ℃. Then pouring the mixture into a freeze-drying container (see figure 2 for container structure diagram) with occupation holes, wherein the movable occupation columns 3 in the stainless steel mold 4 in the container are distributed in 5 rows and 7 columns, and the diameter of each movable occupation column 3 is 2mm, and the height of each movable occupation column is 20mm. Putting the container into a liquid nitrogen tank, standing for 8 hours, and taking out the container for later use after the solution is completely frozen; the process can obtain even and compact prefreezing structure of the porous skin repairing film.
3) After the step 2 is finished, dissolving polyethylene glycol, preparing 8wt% of PEG-400 solution, putting the solution into a water bath at the temperature of 80 ℃, and stirring for 6 hours to dissolve the solution for later use.
4) And (3) after the step 3 is finished, pouring the product onto the frozen body in the step 2, taking out the occupying columns of the freeze-drying mold when pouring is noticed, and then pouring the solution obtained in the step 3 into the holes. And (4) after the solution in the step (3) is filled, putting the whole system into a liquid nitrogen tank, and standing for 16 hours.
5) And (5) after the step (4) is finished, freeze-drying the frozen product. The method comprises the following specific steps of a) controlling the temperature of a freeze dryer to be-55 ℃ and the time to be 3h; b) The temperature of a clapboard of the freeze dryer is-15 ℃ and the time is 8 hours; c) The temperature of a clapboard of the freeze dryer is 2 ℃, and the time is 18h; d) The temperature of a clapboard of the freeze dryer is 16 ℃, and the time is 3 hours; through the freeze-drying process, the product can be completely freeze-dried.
6) And (5) after the step (5) is finished, putting the reaction product into a bubble cap and an aluminum-plastic bag, and performing sterilization treatment to obtain a finished product, wherein the structure diagram of the finished product is shown in figure 1.
The sterilization mode mentioned in step 6 is electron beam irradiation sterilization, and the sterilization dose is 19k.
Example 3
1) 1g of collagen and 2g of gelatin were added to a beaker, and 97ml of purified water was added thereto to swell the mixture for 16 hours, followed by heating to 90 ℃ and slowly adding 3g of carboxymethyl chitosan while stirring.
2) After the solution in the step 1) is dissolved, 0.5g of ionic crosslinking agent stannic chloride is added, and the reaction is carried out for 4 hours at 55 ℃. Then pouring the mixture into a freeze-drying container (container structure diagram, see figure 2) with occupation holes, wherein the movable occupation columns 3 in the stainless steel mold 4 in the container are distributed in 5 rows and 7 columns, the diameter of each movable occupation column 3 is 2mm, and the height of each movable occupation column is 20mm. Putting the container into a liquid nitrogen tank, standing for 5 hours, and taking out the container for later use after the solution is completely frozen; the process can obtain uniform and compact porous skin repairing film pre-freezing structure.
3) And (3) after the step 2 is finished, dissolving polyethylene glycol, preparing 8wt% of polyethylene glycol-2000 solution, putting the solution into a water bath at the temperature of 60 ℃, and stirring for 2 hours to dissolve the solution for later use.
4) And (4) pouring the product onto the frozen body obtained in the step (2) after the step (3) is finished, taking out the occupying columns of the freeze-drying mold when pouring, and then pouring the solution obtained in the step (3) into the holes. And (4) after the solution in the step (3) is filled, putting the whole system into a liquid nitrogen tank, and standing for 1-24 hours.
5) After step 4 is completed, the frozen product is subjected to vacuum drying under vacuum conditions: the temperature is controlled at 30 ℃ and vacuum drying is carried out for 48 hours.
6) And (5) after the step 5 is finished, putting the reaction product into a bubble cap and an aluminum-plastic bag, and performing sterilization treatment to obtain a finished product.
The sterilization mode mentioned in step 6 is ethylene oxide sterilization.
Example 4
The embodiment provides a preparation method of a skin repairing film capable of degrading and generating a breathing hole, which comprises the following steps:
1) 2g of gelatin powder was added to a beaker, 98ml of purified water was added thereto to swell the mixture for 5 hours, the mixture was heated to 70 ℃ and 1.5g of sodium carboxymethyl starch was slowly added thereto with stirring.
2) After the solution in the step 1) is dissolved, 0.1g of barium sulfate serving as an ionic crosslinking agent is added, and the reaction is carried out for 4 hours at 55 ℃. Then pouring the mixture into a freeze-drying container (see figure 2 for container structure diagram) with occupation holes, wherein the movable occupation columns 3 in the stainless steel mold 4 in the container are distributed in 5 rows and 7 columns, and the diameter of each movable occupation column 3 is 2mm, and the height of each movable occupation column is 20mm. Placing the container into a liquid nitrogen tank, standing for 7 hours, and taking out the container for later use after the solution is completely frozen; the process can obtain even and compact prefreezing structure of the porous skin repairing film.
3) And (3) after the step 2 is finished, dissolving polyethylene glycol, preparing 9wt% of PEG-6000 solution, putting the solution into a water bath at 70 ℃, and stirring for 5 hours to dissolve the solution for later use.
4) And (3) after the step 3 is finished, pouring the product onto the frozen body in the step 2, taking out the occupying columns of the freeze-drying mold when pouring is noticed, and then pouring the solution obtained in the step 3 into the holes. And (4) after the solution in the step (3) is filled, putting the whole system into a liquid nitrogen tank, and standing for 10 hours.
5) And (5) after the step (4) is finished, performing ethanol dehydration drying on the frozen product, wherein the drying mode can adopt various modes such as freeze vacuum drying, ethanol dehydration and the like.
The ethanol dehydration method specifically comprises adding the product into 75-100% ethanol, and ultrasonic cleaning for 1-5 hr. After the end, the ethanol is poured out, and then the operation is repeated for 1 to 3 times until the product is soft. The alcohol treated product was then placed in an air-forced drying oven set at 20-50 deg.C and dried for 10-24 hours to remove the ethanol.
6) And (5) after the step 5 is finished, putting the reaction product into a bubble cap and an aluminum-plastic bag, and performing sterilization treatment to obtain a finished product.
The sterilization mode mentioned in step 6 is cobalt-60 irradiation sterilization, and the irradiation dose is 25k.
Example 5
1) 1g of collagen and 2g of gelatin are added into a beaker, 97ml of purified water is added for swelling for 16 hours, then the temperature is heated to 90 ℃, and 3g of other high polymer materials, namely carboxymethyl chitosan, are slowly added under the stirring state.
2) After the solution in the step 1) is dissolved, 0.5g of ionic crosslinking agent stannic chloride is added, and the reaction is carried out for 4 hours at 55 ℃. Then pouring the mixture into a freeze-drying container (container structure diagram, see figure 2) with occupation holes, wherein the movable occupation columns 3 in the stainless steel mold 4 in the container are distributed in 5 rows and 7 columns, the diameter of each movable occupation column 3 is 2mm, and the height of each movable occupation column is 20mm. Putting the container into a liquid nitrogen tank, standing for 5 hours, and taking out the container for later use after the solution is completely frozen; the process can obtain uniform and compact porous skin repairing film pre-freezing structure.
3) And (3) after the step 2 is finished, dissolving polyethylene glycol, preparing 8wt% of polyethylene glycol-2000 solution, putting the solution into a water bath at the temperature of 60 ℃, and stirring for 2 hours to dissolve the solution for later use.
4) And (3) after the step 3 is finished, pouring the product onto the frozen body in the step 2, taking out the occupying columns of the freeze-drying mold when pouring is noticed, and then pouring the solution obtained in the step 3 into the holes. And (4) after the solution in the step (3) is filled, putting the whole system into a liquid nitrogen tank, and standing for 1-24 hours.
5) And (4) after the step 4 is finished, freeze-drying the frozen product. The method comprises the following specific steps of a) controlling the temperature of a freeze dryer to be-55 ℃ and the time to be 3h; b) The temperature of a clapboard of the freeze dryer is-10 ℃ for 8 hours; c) The temperature of a clapboard of the freeze dryer is 5 ℃, and the time is 12 hours; d) The temperature of a clapboard of the freeze dryer is 16 ℃, and the time is 6 hours; through the freeze-drying process, the product can be completely freeze-dried.
6) And (5) after the step 5 is finished, putting the reaction product into a bubble cap and an aluminum plastic bag, and performing sterilization treatment to obtain a finished product.
The sterilization means mentioned in step 6 may be ethylene oxide sterilization.
Product performance testing
The samples of examples 1-5 above were selected for comparison with two common hemostatic materials, the comparative sample being "Haao" skin repair film from Zhenghai biology, inc., and "gelatin sponge" from Nanjing Jinling. The liquid absorption capacity, the degradation capacity and the like of the product are respectively tested and compared.
1. Liquid absorption Performance test
The samples of examples 1-5 were taken with two controls and the initial weight M was weighed 0 Then the sample is put into different liquids and the mass M is weighed after the complete imbibition h Imbibition capacity of M h /M 0 The calculated value of (a). During imbibition and recording the imbibition time T at which the absorption is saturated. The absorbed liquid is 0.9% normal saline, PBS buffer solution and anticoagulated rabbit blood. The results of the liquid absorption performance test of each sample are summarized in table 1.
TABLE 1
The best imbibition performance of example 3 was analyzed from the table above.
2. Test for degradation Properties
The samples of examples 1-5 were taken with two controls by mass: the three liquids are added into a constant temperature incubator with the volume of 1 ℃ +/-1 ℃ for 30 days respectively, and the degradation effect is observed. The degradation effect is shown in Table 2
TABLE 2
It can be seen from table 2 above that formulations 2 and 3 are well degraded, pores are formed at the right time when pores are required to be formed, and the sample is degraded at 30 days, which is just consistent with the skin repair time.
Claims (9)
1. The skin repairing membrane capable of degrading to generate the breathing holes is characterized by being of a sponge structure, the raw materials of the skin repairing membrane comprise a material liquid 1, a material liquid 2 and an ionic cross-linking agent, the skin repairing membrane comprises a skin simulation structure and a plurality of degradation column structures supported inside the skin simulation structure, the skin simulation structure is made of the material liquid 1 and the ionic cross-linking agent, the degradation column structures are made of the material liquid 2, and the material liquid 1 is made of collagen, gelatin and/or other high polymer materials and water; the feed liquid 2 is prepared from polyethylene glycol and water, the mass ratio of the feed liquid 1 to the feed liquid 2 to the ionic crosslinking agent is (1-10): 1-10: (0.1-0.2), and the ratio of collagen, gelatin and/or other high polymer materials to water in the feed liquid 1 is 1-6g:94 to 99ml, wherein the mass ratio of polyethylene glycol to water in the feed liquid 2 is 2 to 10:90 to 98.
2. The skin repair film capable of degrading to produce a breathing hole according to claim 1, wherein the collagen is at least one of bone collagen and skin collagen, the gelatin is at least one of bone gelatin and skin gelatin, and the other polymer material is at least one of sodium carboxymethylcellulose, croscarmellose sodium, polylactic acid, hyaluronic acid, carboxymethyl chitosan, sodium alginate and hyaluronic acid.
3. The dermaplastic membrane capable of degrading to produce respiratory pores as claimed in claim 1, wherein the polyethylene glycol is at least one of PEG-200, PEG-400, PEG-2000 and PEG-6000.
4. The membrane according to claim 1, wherein said membrane is classified into a type, B type, C type and D type, wherein said a type has a thickness of 0.1-0.5mm, a size of 100 x 200mm, 100 x 300mm or 300 x 300mm, said B type has a thickness of 0.5-0.8mm, a size of 50 x 200mm or 100 x 300mm, said C type has a thickness of 0.8-1.2mm, a size of 100 x 100mm or 100 x 200mm, and said D type has a thickness of 1.2-2mm, a size of 100 x 300mm, 300 x 300 or 400 x 400.
5. The preparation method of the skin repair film capable of degrading and producing the breathing hole, based on claim 1, is characterized by comprising the following steps:
dissolving a feed liquid 1, adding an ionic cross-linking agent for cross-linking treatment, reacting for 4 hours at 40-90 ℃, then pouring the solution into a stainless steel container with a position occupying column, then placing the stainless steel container into a liquid nitrogen tank for standing for 1-8 hours until the solution is frozen and frozen, and then taking out for later use;
step two, putting the feed liquid 2 into a water bath at the temperature of 60-90 ℃, stirring for 1-9 hours for dissolving, pouring the dissolved feed liquid 2 into holes, taking out the space occupying columns in the frozen ice prepared in the step one during pouring, putting the whole into liquid nitrogen after pouring is finished, and standing;
step three, drying the frozen product processed in the step two;
and step four, sterilizing the dried reaction product to obtain a finished product.
6. The method for preparing the skin repairing film capable of degrading and producing the breathing hole according to claim 5, wherein the drying mode in the third step is freeze vacuum drying, vacuum drying or ethanol dehydration.
7. The method for preparing the skin repairing film capable of degrading and generating the breathing hole according to claim 6, wherein the vacuum freeze-drying in the third step comprises the following specific steps:
a) Freezing under vacuum at-40 deg.C to-55 deg.C for 1-3 h;
b) Continuously freezing under vacuum at the temperature of-10 to-15 ℃ for 2 to 10 hours;
c) Continuously freezing under vacuum at 0-5 deg.C for 12-24 h;
d) Continuously freezing under vacuum at the temperature of 15 to 25 ℃ for 1 to 5 hours, and completely freezing the final product;
the vacuum drying method is that under the vacuum condition, the temperature is controlled at 20 to 30 ℃, and the vacuum drying is carried out for 20 to 48 hours;
the ethanol dehydration method comprises the steps of putting a product into 75-100wt% ethanol, putting the product into an ultrasonic cleaning machine, performing ultrasonic cleaning for 1-5h, pouring off the ethanol after the ultrasonic cleaning is finished, repeating the step for 1-3 times until the product is softened, then putting the treated product into a forced air drying oven, setting the temperature to be 20-50 ℃, and drying for 10-24h to remove the ethanol.
8. The method for preparing a skin repair membrane capable of degrading and generating a breathing hole according to claim 5, wherein the sterilization treatment in the fourth step is at least one of cobalt-60 irradiation sterilization, electron beam irradiation sterilization and ethylene oxide sterilization.
9. The use method of the skin repair film capable of degrading and generating the breathing hole, which is based on claim 1, is characterized by comprising the following steps: the skin repairing film capable of degrading to generate the breathing holes is applied to the skin wound of a patient after being wholly or cut, normal saline is properly dripped if needed, the wound is washed, then the wound is fixed by using a medical adhesive tape to prevent slipping, and after 30 days, the medical adhesive tape is taken down.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118059301A (en) * | 2024-04-19 | 2024-05-24 | 中国人民解放军总医院第四医学中心 | Preparation method of difunctional membrane for periodontal bone tissue repair |
| CN118059301B (en) * | 2024-04-19 | 2024-07-02 | 中国人民解放军总医院第四医学中心 | Preparation method of difunctional membrane for periodontal bone tissue repair |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118059301A (en) * | 2024-04-19 | 2024-05-24 | 中国人民解放军总医院第四医学中心 | Preparation method of difunctional membrane for periodontal bone tissue repair |
| CN118059301B (en) * | 2024-04-19 | 2024-07-02 | 中国人民解放军总医院第四医学中心 | Preparation method of difunctional membrane for periodontal bone tissue repair |
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