CN115728476A - Multi-parameter platelet function analyzer - Google Patents
Multi-parameter platelet function analyzer Download PDFInfo
- Publication number
- CN115728476A CN115728476A CN202211514008.0A CN202211514008A CN115728476A CN 115728476 A CN115728476 A CN 115728476A CN 202211514008 A CN202211514008 A CN 202211514008A CN 115728476 A CN115728476 A CN 115728476A
- Authority
- CN
- China
- Prior art keywords
- blood
- tested
- test
- injector
- processing element
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004369 blood Anatomy 0.000 claims abstract description 146
- 239000008280 blood Substances 0.000 claims abstract description 146
- 238000012360 testing method Methods 0.000 claims abstract description 103
- 238000012545 processing Methods 0.000 claims abstract description 67
- 239000000126 substance Substances 0.000 claims abstract description 56
- 238000002347 injection Methods 0.000 claims abstract description 39
- 239000007924 injection Substances 0.000 claims abstract description 39
- 208000007536 Thrombosis Diseases 0.000 claims description 21
- 230000000740 bleeding effect Effects 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 230000007704 transition Effects 0.000 claims description 15
- 238000004458 analytical method Methods 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 3
- 239000013013 elastic material Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 210000001772 blood platelet Anatomy 0.000 description 76
- 208000032843 Hemorrhage Diseases 0.000 description 20
- 208000034158 bleeding Diseases 0.000 description 18
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 10
- 102000008186 Collagen Human genes 0.000 description 8
- 108010035532 Collagen Proteins 0.000 description 8
- 229920001436 collagen Polymers 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 108010047303 von Willebrand Factor Proteins 0.000 description 8
- 102100036537 von Willebrand factor Human genes 0.000 description 8
- 108010049003 Fibrinogen Proteins 0.000 description 7
- 102000008946 Fibrinogen Human genes 0.000 description 7
- 230000004913 activation Effects 0.000 description 7
- 230000002776 aggregation Effects 0.000 description 7
- 238000004220 aggregation Methods 0.000 description 7
- 229940012952 fibrinogen Drugs 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 108020003175 receptors Proteins 0.000 description 6
- 102000005962 receptors Human genes 0.000 description 6
- 108091006027 G proteins Proteins 0.000 description 5
- 102000030782 GTP binding Human genes 0.000 description 5
- 108091000058 GTP-Binding Proteins 0.000 description 5
- 102100038394 Platelet glycoprotein VI Human genes 0.000 description 5
- 101710194982 Platelet glycoprotein VI Proteins 0.000 description 5
- 230000023555 blood coagulation Effects 0.000 description 5
- 230000023597 hemostasis Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000002861 ventricular Effects 0.000 description 5
- 208000024172 Cardiovascular disease Diseases 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 230000015271 coagulation Effects 0.000 description 4
- 238000005345 coagulation Methods 0.000 description 4
- 230000002439 hemostatic effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 102100025306 Integrin alpha-IIb Human genes 0.000 description 3
- 101710149643 Integrin alpha-IIb Proteins 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000002618 extracorporeal membrane oxygenation Methods 0.000 description 3
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 2
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 208000015957 Acquired Von Willebrand disease Diseases 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- 101100425949 Mus musculus Tnfrsf13c gene Proteins 0.000 description 1
- 208000035965 Postoperative Complications Diseases 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 102000019997 adhesion receptor Human genes 0.000 description 1
- 108010013985 adhesion receptor Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 108010083526 asialo-von Willebrand Factor Proteins 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 229940019700 blood coagulation factors Drugs 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000036996 cardiovascular health Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 208000020832 chronic kidney disease Diseases 0.000 description 1
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000000541 pulsatile effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229960001134 von willebrand factor Drugs 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a multi-parameter platelet function analyzer, which comprises a blood injection unit, a blood treatment unit and an observation unit, wherein for a patient who uses blood to contact medical equipment, the blood of the patient is collected as blood to be tested, after the blood to be tested is subjected to test pretreatment, a second treatment element is connected with an injector, and then the blood to be tested is injected into a test cavity by using an injection pump and the injector; for a patient who does not use blood to contact medical equipment, after test pretreatment is carried out on blood to be tested, the first processing element is connected with the second processing element, the first processing element is connected with the injector, then the blood to be tested is injected into the microchannel by using the injection pump and the injector, the blood to be tested is subjected to non-physiological high shear stress in the microchannel and then enters the test cavity, and after the blood to be tested flows through the test substance, the adhesion condition of platelets on the test substance is observed by using the observation unit.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a multi-parameter platelet function analyzer.
Background
Cardiovascular disease (CVD), pulmonary disease and chronic renal failure affect people in China. In 2021, "Chinese cardiovascular health and disease report" indicates that the prevalence of cardiovascular diseases in China is in an ascending stage, about 3.3 million people suffer from cardiovascular diseases, and about millions of people die from the cardiovascular diseases every year. BCMDs (blood contacting medical devices) including artificial hearts, blood pumps, extracorporeal lung oxygenators (ECMOs), mechanical valves, etc., are often used to treat these diseases. In recent years, BCMDs have evolved from simple medical devices to more advanced medical devices, and can be applied to various organs.
Complications related to equipment (such as thrombus, bleeding and the like) are still the main problems faced in the present stage, and the occurrence of the complications can seriously restrict the recovery of patients and increase the death rate; on the other hand, the economic burden of the treatment of the patient is increased, and the treatment cost of the complication accounts for a large proportion of the medical cost of the patient who uses the blood contact medical equipment. Although patients who use BCMD receive anticoagulant therapy to prevent the occurrence of thrombosis, stroke occurs in 0.7% to 3.0% of patients each year. Although the incidence of adverse events in patients using small continuous flow ventricular assist devices (artificial hearts) is reduced compared to earlier pulsatile flow ventricular assist devices (artificial hearts), patient bleeding (30%) and thrombosis (4% to 11%) remain common post-operative complications of small continuous flow ventricular assist devices. Acquired von willebrand disease (AvWF) has been reported in almost all patients using small continuous flow ventricular assist devices. The results of the Lazar et al study showed that 86% of patients with ECMO support developed thrombus or hemorrhage, or both (31%). Bleeding complications were found in 73% of patients receiving ECMO support for more than 28 days in a study by Gupta et al.
Non-physiological high shear stress (NPSS) is present in some blood-contacting medical devices, such as artificial hearts, leaflet regions of ventricular assist devices and hinge regions of mechanical valves, and can cause serious damage to blood, especially to important blood coagulation factor platelets, which can directly affect the blood coagulation function of patients, causing thrombosis or bleeding. Shear force damage to platelets can be manifested in two ways, 1) non-physiological high shear forces can cause activation of platelets, and more activated platelets will increase platelet adhesion and aggregation, which will lead to thrombus formation; 2) Non-physiological shear forces may impair the normal hemostatic function of platelets, eventually leading to bleeding.
Platelets are an important cell in blood and play a key role in the process of hemostasis. Platelets are in the vicinity of damaged blood vessels and adhesion to associated hemostatic proteins is a critical initial step in the process of hemostasis. At the site of vascular injury, the subendothelial matrix protein layer, especially vWF factor (von willebrand factor) and collagen, is exposed to the blood. Exposed vWF factor and collagen bind to the platelet adhesion receptor Glycoproteins (GP), ib α and GPVI (glycoprotein VI), respectively, initiating the clotting process. In addition, fibrinogen mediates platelet-platelet interactions (aggregation), playing a critical role in both platelet adhesion and hemostasis. GPIIb/IIIa on the surface of platelets is the major binding receptor for fibrinogen.
NPSS greater than 100Pa was found to cause shedding of platelet surface GPIb α and GPVI receptors. Since GPIb α binds to vWF factor and GPVI binds to collagen, both play key roles in adhesion of platelets to matrix proteins (vWF and collagen) in the subendothelium of blood vessels. Therefore, when GPIb α and GPVI are exfoliated, the adhesion of platelets to vWF and collagen is reduced, which may lead to platelet coagulation dysfunction, affect the hemostatic process, and more likely lead to bleeding. Clinical studies have shown that GPIb α shedding is directly associated with non-surgical bleeding in heart prosthesis patients, and that most patients with low expression levels of GPVI receptors on platelets are also characterized by mild bleeding. In addition, NPSS can cause GPIIb/IIIa activation on the platelet surface and binding to fibrinogen, causing aggregation between platelets, possibly initiating thrombosis. Platelet ADP (adenosine diphosphate) receptors (P2Y 12 receptors) can couple to G proteins, inducing platelet aggregation. If the GPIIb/IIIa receptor on the surface of platelets is activated under the induction of NPSS, a large amount of fibrinogen will bind to the platelets, which will increase the risk of thrombosis.
At present, the instruments for clinically detecting the activation and aggregation of platelets mainly comprise an activated blood coagulation time detector and a platelet analyzer. The activated blood coagulation time detector is mainly used for detecting blood coagulation time, reflects the state of a patient according to the length of the blood coagulation time, and cannot directly reflect the damage state of platelets, the change of adhesion capacity and the like. The platelet analyzer can only analyze the number of platelets and the aggregation function of the platelets and cannot reflect the change of the adhesion capacity of the platelets and hemostasis-related proteins.
Disclosure of Invention
The invention aims to provide a multi-parameter platelet function analyzer, which solves the problems in the prior art, can analyze the activation degree of platelets and the adhesion capacity of coagulation-related proteins, is convenient for analyzing the change of platelet adhesion hemostasis capacity of patients, and provides convenience for evaluating the probability of thrombosis or hemorrhage of the patients.
In order to achieve the purpose, the invention provides the following scheme: the invention provides a multi-parameter platelet function analyzer, comprising:
the blood injection unit comprises an injection pump and an injector, and the injection pump can push a piston of the injector to move;
the blood treatment device comprises a blood treatment unit, a first treatment unit and a second treatment unit, wherein the first treatment unit is provided with a micro-channel which can enable blood to be tested to be subjected to non-physiological high-shear stress, the second treatment unit is provided with a test cavity, a test substance is arranged in the test cavity, the first treatment unit and the second treatment unit can be connected with an injector, and the micro-channel and the test cavity can be communicated with an injection port of the injector; the first processing element and the second processing element are detachably connected, and when the first processing element is connected with the second processing element, the injection port of the injector is communicated with the test cavity by using the micro flow channel;
an observation unit for observing the test substance after the blood to be tested has flowed through.
Preferably, the micro flow channel is a flow channel with a cuboid structure, the height of the micro flow channel is 0.01mm-1mm, and the width of the micro flow channel is 10 times-50 times of the height of the micro flow channel.
Preferably, when the first treatment element is connected with the second treatment element, the first treatment element is connected with the second treatment element by using a connecting sleeve, and the connecting sleeve is made of an elastic material.
Preferably, the second processing element further comprises a transition cavity, one end of the transition cavity with a larger cross-sectional area is communicated with the test cavity, and one end of the transition cavity with a smaller cross-sectional area can be communicated with the micro flow channel.
Preferably, the blood processing unit further comprises a plurality of carrying sheets, the test substance is disposed on the carrying sheets, the carrying sheets are arranged at equal intervals, and the arrangement direction of the carrying sheets is perpendicular to the length direction of the micro flow channel.
Preferably, the blood treatment unit further comprises a reservoir, the reservoir is communicated with the second treatment element and is positioned on the side of the second treatment element far away from the blood injection unit, and the reservoir can contain waste liquid led out by the second treatment element.
Preferably, the second processing element comprises a box body and a box cover, the box cover is detachably connected with the box body, a sealing element is arranged between the box cover and the box body, and the box cover is made of transparent materials.
Preferably, the observation unit includes a laser diode, an objective lens and a photodetector, the objective lens is located on the top of the test substance, the laser diode faces the test substance, the photodetector is located on the top of the objective lens, and the objective lens and the photodetector cooperate to scan the test substance through which the blood to be tested flows.
Preferably, the multiparameter platelet function analyzer further comprises an analysis unit, wherein the analysis unit comprises a converter and an analyzer, the converter is connected with the photoelectric detector, and the converter is in communication connection with the analyzer.
Preferably, for a patient who uses a blood contact medical device, blood of the patient is collected as the blood to be tested, after the blood to be tested is subjected to pre-test treatment, a second treatment element is connected with the injector, then the blood to be tested is injected into the test cavity by using the injection pump and the injector, after the blood to be tested flows through the test substance, the adhesion condition of platelets on the test substance is observed by using an observation unit, and the observation unit is compared with a normal threshold value to evaluate the occurrence probability of bleeding or thrombus of the patient;
for a patient who does not use blood to contact medical equipment, blood of the patient is collected to serve as the blood to be tested, after test pretreatment is carried out on the blood to be tested, the first treatment element is connected with the second treatment element, the first treatment element is connected with the injector, then the injection pump and the injector are used for injecting the blood to be tested into the micro flow channel, the blood to be tested is subjected to non-physiological high-shear stress in the micro flow channel and then enters the test cavity, after the blood to be tested flows through the test substance, the adhesion condition of platelets on the test substance is observed by the observation unit and compared with a normal threshold value, and the occurrence probability of bleeding or thrombus of the patient is evaluated.
Compared with the prior art, the invention has the following technical effects: the multi-parameter platelet function analyzer comprises a blood injection unit, a blood treatment unit and an observation unit, wherein the blood injection unit comprises an injection pump and an injector, and the injection pump can push a piston of the injector to move; the blood processing unit comprises a first processing element and a second processing element, the first processing element is provided with a micro channel, the micro channel can enable blood to be tested to be subjected to non-physiological high shear stress, the second processing element is provided with a testing cavity, a testing substance is arranged in the testing cavity, the first processing element and the second processing element can be connected with an injector, and the micro channel and the testing cavity can be communicated with an injection port of the injector; the first processing element is detachably connected with the second processing element, and when the first processing element is connected with the second processing element, the injection port of the injector is communicated with the test cavity by using the micro-channel; the observation unit is used for observing the test substance after the blood to be tested flows through.
For a patient who uses a blood contact medical device, collecting blood of the patient as blood to be tested, performing test pretreatment on the blood to be tested, connecting a second treatment element with an injector, injecting the blood to be tested into a test cavity by using an injection pump and the injector, observing the adhesion condition of platelets on a test substance by using an observation unit after the blood to be tested flows through the test substance, and evaluating the occurrence probability of bleeding or thrombus of the patient; for a patient who does not use blood to contact medical equipment, blood of the patient is collected to serve as blood to be tested, after test pretreatment is carried out on the blood to be tested, the first processing element is connected with the second processing element, the first processing element is connected with the injector, then the blood to be tested is injected into the microchannel by using the injection pump and the injector, the blood to be tested is subjected to non-physiological high-shear stress in the microchannel and then enters the test cavity, after the blood to be tested flows through the test substance, the adhesion condition of platelets on the test substance is observed by using the observation unit, and the occurrence probability of bleeding or thrombus of the patient is evaluated.
The multi-parameter platelet function analyzer is utilized to enable blood to be tested to flow through a test substance, the test substance comprises collagen, fibrinogen, G protein, vWF and BSA (bovine serum albumin), after the blood to be tested flows through the test substance, the adhesion condition of platelets is observed by an observation unit, the adhesion result is analyzed, the adhesion condition of the blood of a healthy donor can be compared when the adhesion result is analyzed, and finally, the incidence probability of bleeding or thrombosis complications of a patient is judged by comparing the adhesion condition of the blood with a normal range threshold value. The multi-parameter platelet function analyzer firstly performs a large number of adhesion experiments on blood of a plurality of healthy donors to obtain a normal adhesion range of normal platelets on test substances including collagen, fibrinogen, G protein, vWF, BSA and the like as a risk assessment reference value, and can obtain the change of the adhesion capacity of blood to be tested on different hemostatic proteins by comparing adhesion conditions, thereby assessing the risk of thrombosis and bleeding complications of a patient to be tested.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic view of the multi-parameter platelet function analyzer of the present invention;
fig. 2 is a schematic structural view of a first processing element and a second processing element of the multiparameter platelet function analyzer according to the present invention.
Wherein 100 is a blood injection unit, 200 is a blood treatment unit, 300 is an observation unit, and 400 is an analysis unit;
the device comprises a syringe pump 1, an injector 2, a first processing element 3, a micro flow channel 4, a second processing element 5, a test chamber 6, a transition chamber 7, a bearing plate 8, a liquid storage device 9, a laser diode 10, an objective lens 11, a photoelectric detector 12, a converter 13, an analyzer 14, a buffer tank 15, a guide flow channel 16, a waste liquid flow channel 17 and a resistance valve 18.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a multi-parameter platelet function analyzer, which can solve the problems in the prior art, can analyze the activation degree of platelets and the adhesion capacity of the platelets and coagulation related proteins, and evaluate the risk of thrombosis or bleeding complications of a patient by analyzing the change of the binding capacity of the platelets and the coagulation related proteins of the patient.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description thereof.
Referring to fig. 1-2, fig. 1 is a schematic structural diagram of a multi-parameter platelet function analyzer of the present invention, and fig. 2 is a schematic structural diagram of a first processing element and a second processing element of the multi-parameter platelet function analyzer of the present invention.
The invention provides a multi-parameter platelet function analyzer, which comprises a blood injection unit 100, a blood treatment unit 200 and an observation unit 300, wherein the blood injection unit 100 comprises an injection pump 1 and an injector 2, and the injection pump 1 can push a piston of the injector 2 to move; the blood treatment unit 200 comprises a first treatment element 3 and a second treatment element 5, wherein the first treatment element 3 is provided with a micro-channel 4, the micro-channel 4 can enable the blood to be tested to be subjected to non-physiological high-shear stress, the second treatment element 5 is provided with a test cavity 6, a test substance is arranged in the test cavity 6, the first treatment element 3 and the second treatment element 5 can be connected with an injector 2, and the micro-channel 4 and the test cavity 6 can be communicated with an injection port of the injector 2; the first processing element 3 is detachably connected with the second processing element 5, and when the first processing element 3 is connected with the second processing element 5, the injection port of the injector 2 is communicated with the test cavity 6 by using the micro flow channel 4; the observation unit 300 is used to observe the test substance after the blood to be tested has flowed through.
When the multi-parameter platelet function analyzer is used, for a patient who uses blood to contact medical equipment, blood of the patient is collected to be used as blood to be tested, after test pretreatment is carried out on the blood to be tested, the second processing element 5 is connected with the injector 2, then the blood to be tested is injected into the test cavity 6 by using the injection pump 1 and the injector 2, and after the blood to be tested flows through a test substance, the adhesion condition of platelets on the test substance is observed by using the observation unit 300; for a patient who does not use blood to contact medical equipment, blood of the patient is collected to be used as blood to be tested, after the blood to be tested is subjected to test pretreatment, the first treatment element 3 is connected with the second treatment element 5, the first treatment element 3 is connected with the injector 2, then the blood to be tested is injected into the micro flow channel 4 by using the injection pump 1 and the injector 2, the blood to be tested is subjected to non-physiological high shear stress in the micro flow channel 4 and then enters the test cavity 6, and after the blood to be tested flows through a test substance, the adhesion condition of platelets on the test substance is observed by using the observation unit 300.
According to the multi-parameter platelet function analyzer disclosed by the invention, the injection pump 1 can push the piston of the injector 2, so that blood to be tested smoothly flows into the micro-channel 4 or the testing cavity 6 and the flowing speed of the blood to be tested is controlled, the first processing element 3 and the second processing element 5 are reasonably utilized according to different patients, and the activation degree and the aggregation degree of platelets are analyzed through the adhesion condition of the platelets on a testing substance.
In the present embodiment, the micro flow channel 4 is a flow channel having a rectangular parallelepiped structure, the height of the micro flow channel 4 is 0.01mm to 1mm, the width of the micro flow channel is 10 times to 50 times the height of the micro flow channel, and the blood to be tested is subjected to a non-physiological high shear stress when flowing through the micro flow channel 4, so that the collected blood to be tested does not need to be processed by the first processing element 3 for a patient who has used a blood contact medical device. It should be noted here that the height of the microchannel 4 is a dimension parallel to the vertical direction, and the width of the microchannel 4 is a dimension perpendicular to the direction of the blood to be tested in the microchannel 4. The invention reasonably sets the specification of the micro-channel 4, so that the blood to be tested is subjected to non-physiological high-shear stress when flowing through the micro-channel, thereby ensuring the smooth work of the analyzer.
It should be further noted that, when the first processing element 3 is connected to the second processing element 5, the first processing element 3 is connected to the second processing element 5 by using a connecting sleeve, and the connecting sleeve is made of an elastic material, so that the connection tightness between the first processing element 3 and the second processing element 5 is ensured while the first processing element 3 is connected to the second processing element 5.
Specifically, the second processing element 5 further comprises a transition cavity 7, one end of the transition cavity 7 with a larger cross section area is communicated with the test cavity 6, one end of the transition cavity 7 with a smaller cross section area is communicated with the micro flow channel 4, the transition cavity 7 is further provided with a transition groove 15, the transition groove 15 can play a buffering role, the flowing speed of blood to be tested is reduced, the blood to be tested can be ensured to uniformly flow through a test substance, and the platelet adhesion condition can be conveniently observed subsequently.
In order to place the test substance conveniently, the blood processing unit 200 further comprises a bearing sheet 8, the test substance is arranged on the bearing sheet 8, the number of the bearing sheets 8 is multiple, multiple test substances are convenient to place simultaneously, multiple functions of the blood platelet are observed simultaneously, the multiple bearing sheets 8 are arranged at equal intervals, the second processing element 5 is convenient to clean and maintain, the arrangement direction of the bearing sheets 8 is perpendicular to the length direction of the micro flow channel 4, blood to be tested can flow through multiple test substances, and the test is ensured to be carried out smoothly.
Meanwhile, in order to enable blood to be tested to smoothly flow through the bearing pieces 8 for testing, a guide flow channel 16 is arranged in the second processing element 5, one end of the guide flow channel 16 is communicated with the transition groove 15, the other end of the guide flow channel 16 guides the blood to be tested to each bearing piece 8, a plurality of branches of the guide flow channel 16 are arranged in parallel, the blood to be tested flows out of the transition groove 15 and then is distributed to each bearing piece 8, and after the blood flows through a test substance on the bearing pieces 8, the blood to be tested is convenient to observe and analyze. In addition, BSA protein (bovine serum albumin) is separately provided in the second processing element 5, the BSA protein serves as a control group, a resistance valve 18 is provided at an inlet of the BSA protein, and when the platelet function is detected using other proteins, the channel flowing to the BSA protein is closed by the resistance valve 18, so that blood can be uniformly distributed. When adhesion of platelets to BSA protein is to be detected, the resistance valve 18 is used to open the channel to BSA protein. After the detection is finished, the waste liquid enters the liquid storage device 9 through the waste liquid flow channel 17, and the waste liquid flow channel 17 is positioned at one end of the bearing sheet 8, which is far away from the buffer cavity 7, and is communicated with the liquid storage device 9.
In other embodiments of the present invention, the blood processing unit 200 further includes a liquid storage 9, the liquid storage 9 is communicated with the second processing element 5, and the liquid storage 9 is located at a side of the second processing element 5 away from the injector 2, the liquid storage 9 can contain waste liquid led out by the second processing element 5, and blood after detection (i.e. flowing through the detection substance) flows out from the second processing element 5 and enters the liquid storage 9, so as to be conveniently collected and centrally processed, and avoid environmental pollution.
In this embodiment, second treatment element 5 includes box body and lid, and the lid can be dismantled with the box body and be connected and set up sealing element between the two, guarantees first treatment element 3's leakproofness, and the lid is made by transparent material, is convenient for observe.
More specifically, the observation unit 300 includes a laser diode 10, an objective lens 11 and a photodetector 12, the objective lens 11 is located on the top of the test substance, the laser diode 10 is disposed toward the test substance, the photodetector 12 is disposed on the top of the objective lens 11, and the objective lens 11 and the photodetector 12 cooperate to scan the test substance through which the blood to be tested flows. A whole blood sample collected from a patient is first incubated with platelet fluorescent dye, and after the completion of the incubation, the blood to be tested flows through the test substance, the laser diode 10 and the objective lens 11 are matched to scan the platelet adhesion result.
In order to improve the analysis efficiency, the multiparameter platelet function analyzer further comprises an analysis unit 400, wherein the analysis unit 400 comprises a converter 13 and an analyzer 14, the converter 13 is connected with the photodetector 12, the converter 13 is in communication connection with the analyzer 14, and the converter 13 can convert the optical signal of the observation unit 300 into a digital signal and transmit the digital signal to the analyzer 14 for data analysis. The analyzer 14 obtains the actual measurement coverage rate of the platelets of the blood to be tested on the test substance according to the observation data of the observation unit 300, and compares the actual measurement coverage rate with the pre-stored coverage rate to obtain an analysis result; wherein the pre-existing coverage is data of coverage of the test substance with the normal blood pre-existing in the analysis unit 400.
When the multi-parameter platelet function analyzer is used:
for a patient who has used the blood contact medical device, blood of the patient is collected as blood to be tested, after the blood to be tested is subjected to pre-test treatment, the second treatment element 5 is connected to the syringe 2, then the blood to be tested is injected into the test chamber 6 by using the injection pump 1 and the syringe 2, and after the blood to be tested flows through the test substance, adhesion of platelets on the test substance is observed by using the observation unit 300.
For a patient who does not use blood to contact medical equipment, blood of the patient is collected to be used as blood to be tested, after the blood to be tested is subjected to test pretreatment, the first treatment element 3 is connected with the second treatment element 5, the first treatment element 3 is connected with the injector 2, the blood to be tested is injected into the micro flow channel 4 through the injection pump 1 and the injector 2, the blood to be tested is subjected to non-physiological high shear stress in the micro flow channel 4 and then enters the test cavity 6, after the blood to be tested flows through a test substance, the adhesion condition of platelets on the test substance is observed through the observation unit 300, then the adhesion condition of the platelets on the patient is compared with the platelet adhesion result of a healthy donor, the change of the adhesion capability of the platelets on the patient and proteins such as vWF, collagen, fibrinogen, G protein and G protein is analyzed, and the occurrence risk of thrombosis and bleeding complications of the patient is judged.
It is also noted herein that the first and second processing elements 3, 5 may be sealed in a standard protein solution prior to performing the test to avoid non-specific adhesion of platelets.
The multi-parameter platelet function analyzer can intuitively reflect the activation and aggregation conditions of the platelets of a patient. According to the obtained result, the medical staff can conveniently judge the risk of the patient suffering from thrombus or bleeding, and the medical staff can contribute to the prevention and treatment of the complications of the patient, such as thrombus formation or bleeding.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A multiparameter platelet function analyzer, comprising:
the blood injection unit comprises an injection pump and an injector, and the injection pump can push a piston of the injector to move;
the blood treatment device comprises a blood treatment unit, a first treatment unit and a second treatment unit, wherein the first treatment unit is provided with a micro-channel which can enable blood to be tested to be subjected to non-physiological high-shear stress, the second treatment unit is provided with a test cavity, a test substance is arranged in the test cavity, the first treatment unit and the second treatment unit can be connected with an injector, and the micro-channel and the test cavity can be communicated with an injection port of the injector; the first processing element and the second processing element are detachably connected, and when the first processing element is connected with the second processing element, the injection port of the injector is communicated with the test cavity by using the micro flow channel;
an observation unit for observing the test substance after the blood to be tested has flowed through.
2. The multiparameter platelet function analyzer according to claim 1, wherein: the micro flow channel is a flow channel with a cuboid structure, the height of the micro flow channel is 0.01mm-1mm, and the width of the micro flow channel is 10 times-50 times of the height of the micro flow channel.
3. The multiparameter platelet function analyzer of claim 1, wherein: when the first treatment element is connected with the second treatment element, the first treatment element is connected with the second treatment element by using a connecting sleeve, and the connecting sleeve is made of an elastic material.
4. The multiparameter platelet function analyzer according to claim 1, wherein: the second processing element further comprises a transition cavity, one end of the transition cavity with the larger sectional area is communicated with the test cavity, and one end of the transition cavity with the smaller sectional area can be communicated with the micro-channel.
5. The multiparameter platelet function analyzer according to claim 1, wherein: the blood processing unit further comprises a plurality of bearing pieces, the test substances are arranged on the bearing pieces, the number of the bearing pieces is multiple, the bearing pieces are arranged at equal intervals, and the arrangement direction of the bearing pieces is perpendicular to the length direction of the micro-channel.
6. The multiparameter platelet function analyzer of claim 5, wherein: the blood treatment unit further comprises a liquid storage device, the liquid storage device is communicated with the second treatment element and is positioned on one side, away from the blood injection unit, of the second treatment element, and the liquid storage device can contain waste liquid led out by the second treatment element.
7. The multiparameter platelet function analyzer of claim 1, wherein: the second is handled the component and is included box body and lid, the lid with the box body can be dismantled to connect and set up sealing element between the two, the lid is made by transparent material.
8. The multiparameter platelet function analyzer of claim 1, wherein: the observation unit comprises a laser diode, an objective lens and a photoelectric detector, wherein the objective lens is positioned at the top of the test substance, the laser diode faces the test substance, the photoelectric detector is arranged at the top of the objective lens, and the objective lens and the photoelectric detector are matched to scan the test substance through which the blood to be tested flows.
9. The multiparameter platelet function analyzer of claim 8, wherein: the photoelectric detector also comprises an analysis unit, wherein the analysis unit comprises a converter and an analyzer, the converter is connected with the photoelectric detector, and the converter is in communication connection with the analyzer.
10. A multiparameter platelet function analyzer as defined in any one of claims 1 to 9, wherein:
for a patient who uses a blood contact medical device, collecting blood of the patient as the blood to be tested, connecting a second processing element with the injector after the blood to be tested is subjected to test pretreatment, then injecting the blood to be tested into the test cavity by using the injection pump and the injector, observing the adhesion condition of platelets on the test substance by using an observation unit after the blood to be tested flows through the test substance, and analyzing the bleeding or thrombus probability of the patient after the blood to be tested is compared with a normal threshold value;
for a patient who does not use blood to contact medical equipment, blood of the patient is collected to serve as the blood to be tested, after the blood to be tested is subjected to test pretreatment, the first processing element is connected with the second processing element, the first processing element is connected with the injector, then the blood to be tested is injected into the micro-channel by using the injection pump and the injector, the blood to be tested is subjected to non-physiological high shear stress in the micro-channel and then enters the test cavity, after the blood to be tested flows through the test substance, the adhesion condition of platelets on the test substance is observed by using an observation unit, and after the blood to be tested is compared with a normal threshold value, the bleeding or thrombus probability of the patient is analyzed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211514008.0A CN115728476B (en) | 2022-11-30 | 2022-11-30 | Multi-parameter platelet function analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211514008.0A CN115728476B (en) | 2022-11-30 | 2022-11-30 | Multi-parameter platelet function analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115728476A true CN115728476A (en) | 2023-03-03 |
| CN115728476B CN115728476B (en) | 2023-07-18 |
Family
ID=85299159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211514008.0A Active CN115728476B (en) | 2022-11-30 | 2022-11-30 | Multi-parameter platelet function analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115728476B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009068874A (en) * | 2007-09-11 | 2009-04-02 | Tokai Univ | Platelet aggregation evaluation device and method therefor |
| CN104903728A (en) * | 2012-12-27 | 2015-09-09 | 高丽大学校产学协力团 | Apparatus and method for platelet function and drug response testing based on microfluidic chip |
| JP2020060488A (en) * | 2018-10-11 | 2020-04-16 | 国立大学法人東北大学 | Shear generating device quantitatively applying shear stress to blood, corpuscle, or coagulation related factor |
| CN112924431A (en) * | 2021-03-16 | 2021-06-08 | 华中科技大学 | Microfluid chip and platelet function detection device |
-
2022
- 2022-11-30 CN CN202211514008.0A patent/CN115728476B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009068874A (en) * | 2007-09-11 | 2009-04-02 | Tokai Univ | Platelet aggregation evaluation device and method therefor |
| CN104903728A (en) * | 2012-12-27 | 2015-09-09 | 高丽大学校产学协力团 | Apparatus and method for platelet function and drug response testing based on microfluidic chip |
| JP2020060488A (en) * | 2018-10-11 | 2020-04-16 | 国立大学法人東北大学 | Shear generating device quantitatively applying shear stress to blood, corpuscle, or coagulation related factor |
| CN112924431A (en) * | 2021-03-16 | 2021-06-08 | 华中科技大学 | Microfluid chip and platelet function detection device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115728476B (en) | 2023-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Baskurt et al. | New guidelines for hemorheological laboratory techniques | |
| Kwan et al. | Microfluidic analysis of cellular deformability of normal and oxidatively damaged red blood cells | |
| US20180267021A1 (en) | Methods and devices for assessing cell properties under controlled gas environments | |
| Hoenich et al. | Synthetically modified cellulose: an alternative to synthetic membranes for use in haemodialysis? | |
| Panzer et al. | Loss of high-molecular-weight von Willebrand factor multimers mainly affects platelet aggregation in patients with aortic stenosis | |
| Quinn et al. | Blood: tests used to assess the physiological and immunological properties of blood | |
| FI113206B (en) | Method and diagnostic kit for the diagnosis of certain haemostatic disorders | |
| DE19620443C2 (en) | Method for automatically performing blood tests | |
| Harris et al. | Effect of sample storage on blood crossmatching in horses | |
| Bialkower et al. | Paper diagnostic for direct measurement of fibrinogen concentration in whole blood | |
| Brandao et al. | Impaired red cell deformability in iron deficient subjects | |
| JPH02502487A (en) | Preparation method for preserved whole blood samples | |
| CN115728476A (en) | Multi-parameter platelet function analyzer | |
| Brockhaus et al. | In vitro thrombogenicity testing of pulsatile mechanical circulatory support systems: Design and proof‐of‐concept | |
| Kottke-Marchant et al. | The platelet reactivity of vascular graft prostheses: an in vitro model to test the effect of preclotting | |
| Miescher | Immunohämatologie der Thrombocyten und Leukocyten | |
| Thorsby et al. | Antigens on human fibroblasts demonstrated with HL-A antisera and anti-human lymphocytic sera | |
| Le Devehat et al. | Red blood cell aggregation and disaggregation in diabetes mellitus | |
| Amin et al. | Hematological alterations after acute exposure to hyperbaric oxygen in rats | |
| Shi et al. | A modified Casson equation to characterize blood rheology for hypertension | |
| Iryna | Physiology of the blood system. Methodological instructions for laboratory classes of subject «Animal physiology» for foreign students of the speciality 221 «Veterinary medicine», branch of knowledge 21 «Veterinary Medicine» | |
| Huisman et al. | Mathematical correction of the invitro storage-related increase in erythrocyte mean cell volume of an automated hematology analyzer-the Cell-Dyn 4000 | |
| Lauha et al. | Overview of the Duration of Venous Blood Extraction Against the Number of Platelets in Level III Students of the D-III Program Health Analyst at Bina Mandiri University, Gorontalo | |
| Acorda et al. | In-vitro anticoagulant property of sargassum confusum f. Validum | |
| Al-Shemery | Risk of incident rheumatoid arthritis related to anemia and associated with CRP |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Zengsheng Inventor after: Wang Hongyu Inventor after: Fan Yubo Inventor after: Deng Xiaoyan Inventor after: Liu Haifeng Inventor after: Feng Wentao Inventor before: Chen Zengsheng Inventor before: Wang Hongyu Inventor before: Fan Yubo Inventor before: Deng Xiaoyan |