CN115721780A - Preparation method of burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, product and application thereof - Google Patents
Preparation method of burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, product and application thereof Download PDFInfo
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Abstract
The invention relates to a preparation method of a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, and a product and application thereof. According to the invention, the gromwell oil is carried by the metal organic framework nano particles, and the fiber material of the nano particles rich in the gromwell oil is prepared in an electrostatic spinning manner, so that the inconvenience in use of the traditional gromwell oil is avoided, and the essence of the gromwell oil can be slowly released. The preparation method has simple preparation process and easy control of reaction; the preparation of the material can be carried out by adopting industrial equipment, the stability is good, and the industrial production can be carried out.
Description
Technical Field
The invention relates to a preparation method of a novel skin repair material for promoting burn and scald. In particular to a preparation method of a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, a product and application thereof.
Background
Burn is one of the most common causes of human trauma, and has extremely high morbidity and mortality in low-income countries. Burns can be caused by the following reasons: (1) Scalding by contact with high temperature fire or liquid, solid and gas; (2) Chemical burns caused by cold, strong acid or strong base chemicals; (3) Electric burn caused by X-ray, ultraviolet ray, microwave, etc. or radiation [1] . While heat burns are the most common type of burn among children, adults and elderly patients.
The skin is the largest organ in the human body, can separate the internal environment and the external environment of the body, and has multiple functions of protection, immunity, temperature control, sense communication, metabolism, communication and the like. Burns can damage the integrity of the skin, allowing microorganisms to break through the skin barrier, reducing the protective function of the skin, and increasing the risk of local reproduction of endogenous or exogenous microorganisms and systemic infection. The arnebia oil is a long-standing external medicine for clinically treating burns and scalds, but the arnebia oil has color and is easy to print and dye clothes after being smeared, so the arnebia oil is inconvenient to use.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a preparation method of a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, which has the traditional Chinese medicine slow-release capacity and can promote the burn and scald mouth repair.
Yet another object of the present invention is to: provides a skin repair material product containing the traditional Chinese medicine sustained-release particles and used for promoting burns and scalds.
Yet another object of the present invention is to: provides an application of the product.
The purpose of the invention is realized by the following scheme: a preparation method of a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles comprises the following steps:
(1) Preparing the traditional Chinese medicine sustained-release granules: fully and uniformly mixing metal organic framework Materials (MOFs) with a certain mass with 10mL of lithospermum oil at room temperature, stirring for 12-24h at room temperature, and centrifuging to collect nano-particles rich in the lithospermum oil;
(2) Preparing a spinning solution; mixing arnebia oil nanoparticles, certain concentration of nano zinc oxide (ZnO NPs) or nano titanium dioxide particles (TiO) 2 NPs) and growth factors are dissolved in Silk Fibroin (SF) solution with certain concentration, and spinning solution is obtained after thorough and uniform mixing;
(3) Spinning: injecting the spinning solution into 1 injector with the volume of 10mL, setting the electrospinning pressure to be 15-20 kv, spinning on natural cellulose base cloth at the flow rate of 0.1-1 mL/h, and performing electrostatic spinning within the acceptance distance of 12-20 cm to obtain the composite fiber membrane with the thickness of 0.1-0.5 mm.
The mass of the metal organic frame material is 0.5-10 g.
The metal organic framework material can be any one of zinc-based, iron-based, lanthanide metal-based and other metal organic framework materials.
The ZnO NPs or TiO of the invention 2 The size of the NPs is 10 to 20 nm, and the added ZnO NPs or TiO 2 NPs mass 10 to 50wt%.
The growth factor of the invention can be any one of Epidermal Growth Factor (EGF), vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factor (FGF).
The concentration of the growth factor is 1-100 ng/mL.
The concentration of the SF is 20-30wt%.
The invention provides a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles, which is prepared by any one of the methods.
The invention provides an application of a burn and scald promotion skin repair material containing traditional Chinese medicine slow-release particles in preparation of a skin cell repair promotion material.
The fibrous membrane material prepared in examples 1-3 was prepared into small disks with a diameter of 0.3 mm, sterilized and then spread on the bottom of a 96-well plate. Then, DMEM medium (100 mu L,10% calf serum) containing 2000 human skin fibroblasts (HFF-1 cells) was inoculated into a single well and cultured in a cell culture chamber (37 ℃ C., 5% carbon dioxide (CO) 2 ) Concentration, 95% relative saturation humidity) for 3 days. The medium was discarded and the residual medium was washed out thoroughly with PBS. Single well addition of 100 μ L10% CCK-8 in serum-free DMEM medium, incubator (37 ℃,5% carbon dioxide (CO) 2 ) Concentration, 95% relative saturation humidity) for 1.5 hr, and measuring absorbance of each well at 450nm with an enzyme-labeling instrument at room temperature.
The invention has the advantages that:
(1) The arnebia oil is carried by the metal organic framework nano particles, and the fiber material rich in the arnebia oil nano particles is prepared in an electrostatic spinning mode, so that the inconvenience of using the traditional arnebia oil is avoided, and the essential arnebia oil can be slowly released.
(2) The preparation method provided by the invention has the advantages of simple preparation process and easy control of reaction; the preparation of the material can be carried out by adopting industrial equipment, the stability is good, and the industrial production can be carried out.
Drawings
FIG. 1 is a diagram showing the activity of the fibrous membrane material prepared in examples 1 to 3 in promoting fibroblast proliferation.
FIG. 1 is a diagram showing the activity of the fibrous membrane material prepared in examples 1 to 3 in promoting fibroblast proliferation. As can be seen from fig. 1, compared with the control group, the fibrous membrane materials prepared in examples 1 to 3 of the present application can promote the growth of fibroblasts, and have the efficacy of promoting the skin cell repair of burns and scalds.
Detailed Description
The technical solution of the present invention is further described below by specific examples. The following examples are further illustrative of the present invention and are not intended to limit the scope of the invention.
Example 1
A composite fiber membrane containing traditional Chinese medicine sustained-release particles is prepared by the following steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 5g of zinc-based metal organic framework material ZIF-8 with 10mL of arnebia oil at room temperature, stirring for 12 to 24h at room temperature, and centrifuging to collect arnebia oil-rich nanoparticles;
(2) Preparing a spinning solution; dissolving 1g of arnebia oil nanoparticles, 8 wt% of ZnO NPs and 8 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL injector, setting the electrospinning pressure to be 15kv, spinning on natural cellulose base cloth at the flow rate of 0.6mL/h, and performing electrostatic spinning within a receiving distance of 12 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
Example 2
A composite fiber membrane containing traditional Chinese medicine sustained-release particles is prepared by the following steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 10g of zinc-based metal organic framework material ZIF-8 and 10mL of lithospermum oil at room temperature, stirring for 24 hours at room temperature, and centrifugally collecting the lithospermum oil-rich nanoparticles;
(2) Preparing a spinning solution; dissolving 5g of arnebia oil nanoparticles, 10wt% of ZnO NPs and 10 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL injector, setting the electrospinning pressure to be 15kv, spinning on natural cellulose base cloth at the flow rate of 0.6mL/h, and performing electrostatic spinning within a receiving distance of 15 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
Example 3
A composite fiber membrane containing traditional Chinese medicine sustained-release particles is prepared by the following steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 5g of zinc-based metal organic framework material ZIF-8 with 10mL of lithospermum oil at room temperature, stirring for 24h at room temperature, and centrifugally collecting nanoparticles rich in lithospermum oil;
(2) Preparing a spinning solution; dissolving 4 g of arnebia oil-rich nanoparticles, 10wt% of nano zinc oxide (ZnO NPs) and 5 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL injector, setting the electrospinning pressure to be 15kv, spinning on natural cellulose base cloth at the flow rate of 0.5 mL/h, and performing electrostatic spinning within a receiving distance of 15 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
Application example
Preparing the composite fiber membrane containing the traditional Chinese medicine sustained-release particles prepared in the embodiments 1-3 into a small round piece with the diameter of 0.3 mm, and flatly paving the small round piece at the bottom of a 96-well plate after sterilization; subsequently, DMEM medium (100. Mu.L, 10% calf serum) containing 2000 human skin fibroblasts (HFF-1 cells) was inoculated into a single well in a cell culture chamber (37 ℃,5% carbon dioxide (CO) 2 ) Concentration, 95% relative saturation humidity) for 3 days; the medium was discarded and the residual medium was washed out thoroughly with PBS.
Control group (control): single well addition of 100 μ L10% CCK-8 in serum-free DMEM medium, incubator (37 ℃,5% carbon dioxide (CO) 2 ) Concentration, 95% relative saturation humidity) for 1.5 hr, and measuring absorbance (Abs) at 450nm of each well with a microplate reader at room temperature as shown in fig. 1.
FIG. 1 is a diagram showing the activity of the fibrous membrane material prepared in examples 1 to 3 in promoting fibroblast proliferation. As can be seen from fig. 1, compared with the control group, the fibrous membrane materials prepared in examples 1 to 3 can promote the growth of fibroblasts, and have the efficacy of promoting the repair of skin cells of burns and scalds.
Claims (9)
1. A preparation method of a burn and scald skin repair promoting material containing traditional Chinese medicine slow-release particles is characterized by comprising the following steps:
(1) Preparing the traditional Chinese medicine sustained-release granules: fully and uniformly mixing 0.5-10 g of metal organic framework Materials (MOFs) with 10mL of lithospermum oil at room temperature, stirring at room temperature for 12-24h, and centrifuging to collect nanoparticles rich in lithospermum oil;
(2) Preparing a spinning solution; mixing radix Arnebiae oil nanoparticlesParticles, 10 to 50wt% of nano zinc oxide (ZnO NPs) or nano titanium dioxide particles (TiO) 2 NPs) and growth factors with the concentration of 1-100 ng/mL are dissolved in 20-30wt% Silk Fibroin (SF) solution, and spinning solution is obtained after thorough mixing;
(3) Spinning: injecting the spinning solution into 1 injector with the volume of 10mL, setting the electrospinning pressure to be 15-20 kv, spinning on natural cellulose base cloth at the flow rate of 0.1-1 mL/h, and performing electrostatic spinning within the acceptance distance of 12-20 cm to obtain the composite fiber membrane with the thickness of 0.1-0.5 mm.
2. The method for preparing the burn and scald skin repair material containing the traditional Chinese medicine slow-release particles as claimed in claim 1, wherein the metal-organic framework material is one of zinc-based, iron-based and lanthanide metal-based metal-organic framework materials.
3. The preparation method of the burn and scald skin repair material containing the traditional Chinese medicine slow-release particles as claimed in claim 1, wherein the ZnO NPs or TiO are used as raw materials 2 The NPs have a size of 10 to 20 nm.
4. The method for preparing the burn and scald promoting skin repair material containing the traditional Chinese medicine sustained release particles according to claim 1 is characterized in that the growth factor is any one of Epidermal Growth Factor (EGF), vascular Endothelial Growth Factor (VEGF) and Fibroblast Growth Factor (FGF).
5. The preparation method of the burn and scald skin repair material containing the traditional Chinese medicine slow-release particles according to any one of claims 1 to any one of claims, which is characterized by comprising the following preparation steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 5g of zinc-based metal organic framework material ZIF-8 with 10mL of arnebia oil at room temperature, stirring for 12 to 24h at room temperature, and centrifuging to collect arnebia oil-rich nanoparticles;
(2) Preparing a spinning solution; dissolving 1g of arnebia oil nanoparticles, 8 wt% of ZnO NPs and 8 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL syringe, setting the electrospinning pressure to be 15kv, spinning on the natural cellulose base cloth at the flow rate of 0.6mL/h, and performing electrostatic spinning within a receiving distance of 12 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
6. The preparation method of the burn and scald skin repair material containing the traditional Chinese medicine slow-release particles according to any one of claims 1 to any one of claims, which is characterized by comprising the following preparation steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 10g of zinc-based metal organic framework material ZIF-8 with 10mL of arnebia oil at room temperature, stirring for 24 hours at room temperature, and centrifugally collecting arnebia oil-rich nanoparticles;
(2) Preparing a spinning solution; dissolving 5g of arnebia oil nanoparticles, 10wt% of ZnO NPs and 10 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL injector, setting the electrospinning pressure to be 15kv, spinning on natural cellulose base cloth at the flow rate of 0.6mL/h, and performing electrostatic spinning within a receiving distance of 15 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
7. The preparation method of the burn and scald skin repair material containing the traditional Chinese medicine slow-release particles according to any one of claims 1 to any one of claims, which is characterized by comprising the following preparation steps:
(1) Preparing the lithospermum oil sustained-release granules: fully and uniformly mixing 5g of zinc-based metal organic framework material ZIF-8 with 10mL of lithospermum oil at room temperature, stirring for 24h at room temperature, and centrifugally collecting nanoparticles rich in lithospermum oil;
(2) Preparing a spinning solution; dissolving 4 g of arnebia oil-rich nanoparticles, 10wt% of nano zinc oxide (ZnO NPs) and 5 ng/mL of epidermal growth factor in 30wt% of Silk Fibroin (SF) solution, and thoroughly and uniformly mixing to obtain a spinning solution;
(3) Spinning: and injecting the spinning solution into a 10mL injector, setting the electrospinning pressure to be 15kv, spinning on natural cellulose base cloth at the flow rate of 0.5 mL/h, and performing electrostatic spinning within a receiving distance of 15 cm to obtain a composite fiber membrane with the thickness of 0.5 mm.
8. A burn and scald skin repair promoting material containing traditional Chinese medicine slow release particles, which is characterized by being prepared according to the method of any one of claims 1 to 7.
9. The application of the burn and scald promoting skin repair material containing the traditional Chinese medicine slow-release particles according to claim 8 in preparing a skin cell repair promoting material.
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