CN115721711B - Pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine and preparation method thereof - Google Patents
Pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine and preparation method thereof Download PDFInfo
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- CN115721711B CN115721711B CN202211335608.0A CN202211335608A CN115721711B CN 115721711 B CN115721711 B CN 115721711B CN 202211335608 A CN202211335608 A CN 202211335608A CN 115721711 B CN115721711 B CN 115721711B
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- herpes zoster
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Abstract
The invention belongs to the technical field of vaccines, relates to a pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine with immunogenicity, and in particular relates to a combined vaccine adopting a recombinant glycoprotein of the herpes zoster virus and pneumococcal capsular polysaccharide to be connected by covalent bonds. The invention also relates to a nucleotide sequence for encoding the recombinant protein of the herpes zoster virus, a recombinant protein expression system and a preparation method of the conjugate vaccine. The invention also relates to a pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine, wherein the amino acid sequence of the herpes zoster virus recombinant protein is at least one selected from the amino acid sequence of ORF68 part or the amino acid complete sequence of ORF68 in the gE glycoprotein. The pneumococcal polysaccharide-herpes zoster virus recombinant protein combined vaccine can be used for simultaneously preventing two common senile diseases, namely pneumonia and herpes zoster by using one vaccine product.
Description
Technical Field
The invention belongs to the technical field of vaccines, and relates to a pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine with immunogenicity and a preparation method thereof.
Background
Infections caused by pneumococci are a major cause of morbidity and mortality worldwide. Pneumonia, febrile bacteremia and meningitis are the most common manifestations of invasive pneumococcal disease, and bacterial transmission in the respiratory tract can lead to otitis media, sinusitis or recurrent bronchitis. Non-invasive diseases are generally less severe, but more common, than invasive diseases.
Pneumococcal pneumonia is the most common community bacterial pneumonia in europe and the united states, with an estimated infection of approximately 100 per 10 thousands of adults per year. Febrile bacteremia and meningitis are about 15-19 per 10 ten thousand adults and 1-2 per 10 ten thousand adults, respectively. The risk of developing one or more of these diseases is very high in infants and the elderly, as well as in immunodeficient persons of any age. Even in economically developed areas, the mortality rate of diseases caused by invasive pneumococci is high; adult mortality from pneumococcal pneumonia is about 10-20%, whereas mortality in high risk groups may exceed 50%. Pneumonia is by far the most common cause of death worldwide for pneumococcal infected individuals.
In China, the incidence of pneumonia of the elderly over 65 years old is 1.6% (about 1600 in every 10 thousands of adults), and the incidence of pneumonia increases sharply with age, and the incidence of pneumonia of the elderly over 75 years old is as high as 11.6%; the old has high incidence rate of pneumonia, the course of the pneumonia is long after infection, the old is not easy to recover, and the severe cases can cause death. There are data showing that 46% -76% of community-acquired pneumonia is caused by pneumococci.
Pneumococci are gram-positive bacteria, the outer bacterial membrane is wrapped by a layer of capsular polysaccharide, and the pneumococci are classified by specific antisera according to the chemical structures of different capsular polysaccharides, so that 91 different serotypes of pneumococci are found. Bacterial polysaccharides were found to be T-cell independent antigens, capable of inducing short term immune effects in children and adults, but not in infants. The polysaccharide is able to bind to the major histocompatibility complex (Major histocompatibility complex, MHC), an essential component of antigen presentation and stimulation of T-helper lymphocytes. The polysaccharide was able to stimulate B lymphocytes to produce antibodies without the aid of T-helper lymphocytes. The stimulation of T-independent B lymphocytes results in a lack of memory effects for this antigen-induced immune effect.
The polysaccharide is covalently bound to the carrier protein, converting the T-cell independent antigen to a T-cell dependent antigen. The polysaccharide component of the conjugate, when bound to B cells, activates T-Helper cells, which specifically recognize the polypeptide portion of the conjugate carrier protein. T-Helper cells respond to carrier proteins to amplify polysaccharide antibody production. The vaccine prepared by the technology is pneumococcal polysaccharide conjugate vaccine and can be used for preventing diseases caused by pneumococci. Currently, there is a widely used clinical vaccine against pneumococcal polysaccharide 13 (PCV 13) containing serotype 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F polysaccharides, which is one of the most widely used vaccines worldwide.
Currently, polysaccharide conjugate vaccine products are developed towards multivalent polysaccharide conjugate vaccines, and currently, pneumonia conjugate vaccine products in clinical trials contain 20-valent and 24-valent vaccine products to improve vaccine coverage. However, these conjugate vaccines have a common disadvantage in that the carrier protein does not confer an immunogenic protective function; that is, although conjugated vaccine vectors are capable of stimulating antibodies production by the body, vaccine designers have not been able to utilize antibodies produced by the carrier protein to prevent disease. Tetanus toxoid, diphtheria non-toxic variant toxin, etc. are used as carrier proteins, not because of the protective nature of the antibodies they produce, but from the standpoint of their safety and the ability to enhance the immunogenicity of the polysaccharide in the conjugate. In addition, tetanus toxoid and diphtheria toxoid are already two components of the triple vaccine used for conventional vaccination, so it is not important whether the carrier protein in the conjugate vaccine stimulates the body to produce protective antibodies.
Varicella-zoster virus (VZV) is a ubiquitous human alpha Herpes virus that causes Varicella (variella) and shingles (herps zoter). In human primary VZV infection, it is common in children to cause fever, and varicella, known as varicella, which is a systemic pruritus. Thereafter, VZV is hidden in the dorsal root ganglion. Shingles is a localized, painful, one or a series of herpes lesions on the skin that result from the re-excitation of VZV. Can be developed at any age, but the incidence rate of the traditional Chinese medicine is obviously increased along with the increase of the age, and the traditional Chinese medicine is one of infectious diseases which are easy to be developed by the aged. It is counted that up to 156 ten thousand new herpes zoster cases occur annually in China. Shingles commonly occurs in the lumbosacral portion and the face. Acute pain is severe, which seriously affects the daily life and work of patients. However, the current treatment of herpes zoster is mainly antiviral and symptomatic treatment, and no specific medicine exists.
The VZV virus consists of a linear, double stranded DNA surrounded by a nucleocapsid, and a protein layer separates the nucleocapsid from an ester coating (lipid envelope), which constitutes the major viral glycoprotein. VZV produces about six glycoproteins, now designated gB, gC, gE, gH, gI and gL, which are also expressed on the surface of infected cell membranes during viral propagation. In infected cells, the gE protein is the most produced protein, which is adsorbed non-covalently to gI and can be adsorbed to the Fc fragment of antibody G (IgG). The gB protein is the target for binding of neutralizing antibodies and plays an important role in the entry of viruses into cells. The gH protein has a fusion function, so that the virus can be conveniently spread among cells; gH proteins require the presence of gL proteins for glycosylation and transfer to the cell surface. The gC protein has no functionality for VZV reproduction. The gE gene, open reading frame (Open reading frame, ORF) 68, is in the Us region of the VZV genome, this protein plus signal peptide contains the 623 amino acid sequence, and the gE protein contains the protein body, a hydrophobic anchor region (amino acid residues 546-558), and a C-terminal tail region.
There are two herpes zoster vaccines on the market today, and Kangmiao (Zostavax) from moesadong was marketed in batches in 2006. The vaccine is attenuated live vaccine. The efficacy of Zostavax was 69.8% in the 50-59 year old population, 65.5% in the 60-69 year old population, and only 55.4% in the above 70 year old population.
On 22 th 2019, the shinrix of ghrelin is marketed in China in batches, and the protective efficacy of shinrix on people over 50 years old is 97.2%, and the protective efficacy of shinrix on people over 70 years old is 91.3%. The vaccine is a recombinant protein vaccine of the herpes zoster virus and contains glycoprotein gE and AS01B adjuvant. The Zostavax attenuated live vaccine is distinguished, and only the herpes zoster glycoprotein is contained in the vaccine. Wherein AS01B adjuvant can help to enhance immune response and pneumonia, and can also produce sustained prevention effect. Current studies show that the herpes zoster virus recombinant protein vaccine shintrix can reduce the risk of shingles incidence by more than 90% in all age groups in the united states, and remains effective four years after inoculation. The overall protection effect for the people aged 70 and above reaches 88.8 percent.
The key to the success of subunit vaccines is the selection of effective antigens and the induction of appropriate adaptive immune responses. Unfortunately, immunization with purified protein antigen alone is often insufficient to induce an appropriate immune response; thus, there is a need for immunostimulating components in combination with adjuvants. Another challenge with herpes zoster subunit vaccines is the need to induce CMI, which can have a therapeutic effect, namely limiting the latent VZV virus and preventing herpes zoster from reviving, not just prophylactic humoral immunity. Shangrix contains as its antigen the glycoprotein gE of VZV, which is the most abundant glycoprotein of VZV, containing potential neutralizing epitopes and T cell epitopes, with critical effects on virus propagation and conduction between ganglion cells. Currently, the use of the gE protein AS a vaccine formulation, QS21 and monophosphate A (Monophosphoryl lipid A, MPL A) AS adjuvants has been shown to act synergistically to induce gE production of high frequency CD4+ T cells in liposomes of the AS01B adjuvant system.
The age-appropriate children need to be vaccinated with at least 15 vaccines, the number of times is several times that of the vaccine species, so the development of multiple multivalent vaccines to replace single species of vaccines is a prime task in the vaccine field. Meanwhile, due to the aging of people, only the old people in China have three hundred million, the health of the old people is protected, and the development of vaccine products aiming at the old people is a hot spot in the current vaccine field.
The host employs a variety of active and passive immune mechanisms to combat infections, with active immunity being mediated by the innate and adaptive immune systems. Innate immunity is developed by exposure to evolutionarily conserved molecular structures, so-called Pathogen-associated molecular patterns (PAMPs), which are produced by a variety of infectious disease pathogens. PAMPs recognition is mediated by pattern recognition receptors, including Toll-like receptors (TLRs), nod-like receptors and RIG-I-like receptors. TLRs are one of the most widely studied classes that constitute pattern recognition receptors. The primary characteristics of the innate immune response caused by TLR activation are the production of pro-inflammatory cytokines, chemokines, type I interferons, and antibacterial peptides.
Innate immunity provides the first line of defense against actively infectious pathogens, limiting early proliferation and spread of pathogens in the body. Subsequently, the host initiates an acquired immune response characterized by antigen-specific T cell and B cell expansion, resulting in the production of high affinity antibodies and cytotoxic T cells, providing bactericidal immunity and ensuring long-term memory against subsequent infection. Bacterial DNA is a PAMPs, an illustration of activating the innate immune system. Unmethylated CG dinucleotides occur frequently in procaryotic DNA, but are rare in eucaryotic DNA. Bacterial DNA released during infection is the exposure of TLR-9 receptor expressing cells to these unmethylated CpG Motifs (CpG Motifs) (consisting of a central unmethylated CG dinucleotide plus flanking regions) triggering a protective immune response that the host is able to eliminate pathogens. Synthesis of oligonucleotides containing demethylated CpG motifs (ODNs) mimics bacterial DNA immunostimulatory activity and, after phagocytosis by target cells, cpG ODNs reach the endoplasmic reticulum/post-lysosomal compartment signaling through interaction with TLR-9. B cells and plasmacytoid dendritic cells (pDCs) are the major cell types that are expressed in humans for TLR-9 receptors and respond to CpG stimuli. The experimental result shows that when the pneumococcal polysaccharide vaccine is immunized after two doses of the pneumococcal polysaccharide conjugate vaccine are immunized, the animal PBMCs respond to the stimulation of the pneumococcal polysaccharide due to the CpG adjuvant effect of the TLR9 stimulator, and the release of the proinflammatory cytokines is increased.
The vaccine field product is characterized in that the vaccine can prevent specific pathogen infection, for example, pneumococcal polysaccharide conjugate vaccine can prevent pneumonia, meningitis and tympanitis caused by pneumococcal infection, and the herpes zoster virus recombinant protein vaccine can prevent herpes zoster caused by herpes zoster virus revitalization. In order to achieve the aim that one preparation can prevent multiple pathogen infection, the means adopted at present are combined vaccines, such as diphtheria, pertussis, tetanus triple, diphtheria, pertussis, tetanus, poliomyelitis and haemophilus influenzae quintuples for children, diphtheria, pertussis, tetanus, poliomyelitis, haemophilus influenzae and hepatitis B hexa-unions for children, all of which are prepared by mixing single-product vaccines, and the prevention effect of single vaccine in part of combined vaccine is poor as seen by clinical evaluation at present.
The medical level of human in the current society is continuously improved, the birth rate of population is continuously reduced, and the vaccine market for children is continuously matured and saturated. And the aged population is rapidly increased, and meeting the health requirement of the aged is a new challenge in the field of vaccines. The development of a vaccine that can meet the health needs of the elderly has great social and market value.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention designs a set of synthesis scheme by utilizing antigenicity of carrier proteins in the combined vaccine, so that antibodies generated by the carrier proteins after immunization have a protective effect.
The technical scheme of the invention is as follows:
in a first aspect of the invention, there is provided a pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine having immunogenicity, wherein the protein in the pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine is a herpes zoster virus recombinant glycoprotein having immunogenicity.
Further, the pneumococcal capsular polysaccharide is covalently linked to a recombinant protein of the herpes zoster virus.
Further, the recombinant proteins of the herpes zoster virus are selected from the group consisting of gE glycoproteins.
Further, the recombinant protein amino acid sequence of the herpes zoster virus is selected from at least one of ORF68 partial amino acid sequence or ORF68 full amino acid sequence in the gE glycoprotein.
In one embodiment of the invention, the pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine with immunogenicity is prepared from a gE glycoprotein, and the amino acid sequence of the recombinant protein is shown as SEQ ID No:1 or SEQ ID No:2, said pneumococcal capsular polysaccharide is selected from at least one of serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F.
Further, the pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine with immunogenicity is in the form of water aqua or freeze-dried agent.
Further, the pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine with immunogenicity contains an adjuvant.
Further, the adjuvant is one of CpG, QS21, aluminum phosphate, mixture of CpG and aluminum phosphate, or mixture of QS21 and aluminum phosphate.
In a second aspect of the invention there is provided a nucleotide sequence encoding a recombinant protein of a herpes zoster virus as described in the first aspect.
In a third aspect of the present invention there is provided a recombinant expression vector comprising a nucleotide sequence as described in the second aspect.
The construction method of the recombinant expression vector comprises the following steps:
1) Synthesis of VZV gE target Gene
ORF68 of the gE glycoprotein gene is truncated, the transmembrane region and intracellular region are removed, and the N-terminal 546 amino acids are reserved. The restriction endonuclease EcoRI sequence is added at the C end of the segment gene, the restriction endonuclease XbaI sequence is added at the N end, and the designed nucleotide sequence is synthesized chemically.
2) Plasmid amplification and target gene extraction
The pUC19 plasmid vector is connected with the synthesized gene after double restriction by EcoRI and XbaI, and is led into an amplified host DH5 alpha, and a LB (amp+) agar solid medium is used for screening monoclonal; the monoclonal containing the target gene is inoculated in LB (amp+) liquid culture medium, cultured and amplified at 37 ℃ and 200rpm, and plasmid pUC19-gE is extracted; the plasmid thus extracted was digested with EcoRI and XbaI restriction enzymes, and the target gene fragment gE was recovered.
3) Construction of eukaryotic expression vectors
Double-restriction enzyme digestion of mammalian cell expression plasmid pGN-M comprising CMV promoter and dihydrofolate reductase (DHFR) gene with EcoRI and XbaI restriction enzymes, and recovery of vector DNA fragment; the vector DNA fragment and the target gene fragment are connected in a tail end sticking mode and are led into a DH5 alpha amplification host, and a monoclonal containing eukaryotic expression plasmid pGN-M_gE is obtained through screening; the plasmid was inoculated into LB (amp+) for amplification culture, and the amplified plasmid was extracted and named VZVGE.
In a fourth aspect of the present invention, there is provided an expression method for preparing a recombinant protein of herpes zoster virus using the recombinant expression vector as described in the third aspect, the expression method employing a CHO cell expression system or an insect baculovirus expression system.
In a fifth aspect of the invention, there is provided a method for preparing a pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine having immunogenicity as described in the first aspect, wherein the pneumococcal capsular polysaccharide and the herpes zoster virus recombinant protein are conjugates obtained by chemical synthesis reaction in buffer or organic solvent.
Further, the organic solvent is selected from dimethyl sulfoxide or dimethylformamide.
Further, the chemical synthesis reaction is selected from one of a reductive amine method, a 1-cyano-4-dimethylamino pyridinium tetrafluoroborate method, an adipoyl dihydrazide method or a 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride method.
Further, the preparation method also comprises pretreatment of pneumococcal capsular polysaccharide, expression and purification of recombinant proteins of herpes zoster virus and purification of conjugates.
Further, the polysaccharide pretreatment comprises degradation and activation, and the degradation method is selected from high-pressure homogenizer degradation, acid hydrolysis method or enzyme digestion method.
The invention has the following technical effects:
1) The invention provides an innovative vaccine, namely pneumococcal polysaccharide-herpes zoster virus recombinant protein combined vaccine, which can be used for simultaneously preventing two common senile diseases, namely pneumonia and herpes zoster by using one vaccine product. The two diseases are to be prevented by the old people at present, and meanwhile, the two vaccination procedures are identical, so that the vaccine product design is very suitable for clinical application of the old people.
2) The invention also provides a preparation method of the multivalent pneumococcus-herpes zoster virus recombinant protein combined vaccine, the herpes zoster recombinant protein is an amino acid partial sequence or a complete sequence produced by a gene recombination technology, after the recombinant protein is combined with a novel adjuvant preparation and immunized with animals, a conjugate polysaccharide antigen part can stimulate organisms to generate specific serotype polysaccharide antibodies, and a carrier protein part can stimulate organisms to generate humoral immunity (protective antibodies) and cellular immunity aiming at the herpes zoster viruses, so that after the pneumococcus-herpes zoster virus recombinant protein conjugate vaccine is immunized, the infection of pneumococcus and herpes zoster can be prevented simultaneously.
3) In addition, as can be seen from the specific embodiment, the recombinant protein conjugate vaccine of the 13-valent pneumococcal polysaccharide-herpes zoster virus has better antigenicity enhancing effect on polysaccharide compared with the recombinant protein conjugate vaccine of the 13-valent pneumococcal polysaccharide-CRM 197 due to the use of the recombinant protein of the herpes zoster as carrier protein. Adjuvant-containing 13-valent pneumococcal polysaccharide-gE combined vaccine, under the stimulation of gE antigen or virus, sensitized immune cells produce IFN-gamma; IFN-gamma production by immunocytes sensitized with the 13-valent pneumococcal polysaccharide-gE conjugate vaccine is significantly higher than that of the adjuvant-containing gE vaccine. The above results demonstrate that the recombinant protein conjugate vaccine of pneumococcal polysaccharide 13-herpes zoster virus of the invention has unexpected technical effect of synergy.
Drawings
FIG. 1 shows the change in cell activation rate during gE cell culture in a bioreactor.
FIG. 2 shows the variation in the production of gE proteins during gE cell culture in a bioreactor.
Detailed Description
The invention has been summarized above, and the following specific examples may be read for further detailed description and understanding. These examples are described for the purpose of illustrating the invention only and are not intended to limit the invention to the scope of these examples.
Embodiment one: pneumococcal serotype 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19F, 23F capsular polysaccharide preparation
Primary seed and working seed preparation
Pneumococcal serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19F and 23F were purified from fermentation broths of capsular polysaccharides by the following methods:
pneumococcal serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19F and 23F were obtained from american type culture collection (American Type Culture Collection), the seed tube was inoculated with 5mL of yeast (BD) -acid casein (BD) broth, incubated at 36 ℃ for 16 hours, when the bacteria grew to an OD600 reading of 1.0, the broth was inoculated into 150mL of fresh yeast-acid casein broth, incubated at 36 ℃ ± 2 ℃ for 8 hours to an exponential growth phase, the incubation stopped, sub-packaging lyophilized, and stored at 4 ℃ as the primary seed.
Pneumococcal serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19F and 23F were inoculated in 5mL yeast-acid casein culture medium, incubated at 36℃for 16 hours, when the bacteria grew to an OD600 reading of 1.0, the bacterial solution was transferred to 150mL fresh yeast-acid casein culture medium, incubated at 36℃for 8 hours to an exponential growth phase, the incubation was stopped, sub-packaging lyophilized, and the working seeds were stored at 4℃as serotypes.
(II) bacterial fermentation
The seed tube was removed from the working seed pool and inoculated into 5mL of yeast-acid casein culture solution and cultured at 36℃to mid-bacterial growth index. Inoculating the bacterial liquid into 150mL of fresh yeast-acid casein hydrolysate, culturing at 36 ℃ for 8 hours to exponential growth phase, inoculating 50mL of bacterial liquid into 2L of yeast-acid casein hydrolysate,
at 36℃to the middle of the exponential growth phase, a fermentation seed liquid was prepared, and the fermentation seed liquid was inoculated into a 50L fermenter containing 30 liters of a yeast-acid casein culture liquid. Sodium hydroxide was used to maintain the pH of the broth at 6.8 until bacteria grew to the late exponential phase.
(III) capsular polysaccharide purification
1. Adding phosphoric acid (Sigma-Aldrich) to adjust the pH of the fermentation broth to 3-5, and stirring for 1 hour;
2. Centrifuging at 9600rpm by a disc centrifuge (Alfava), collecting the supernatant, and discarding the residue discharge part;
3, micro-filtering the centrifugate by a micro-filtration membrane (Millipore) to remove residual cell fragments and insoluble small particulate matters, micro-filtering fermentation centrifugate by a 0.22 mu m membrane (Kebaite), and collecting filtrate;
4, concentrating and washing the micro-filtrate by adopting a 100kD membrane package (Millipore) to obtain a crude bacterial capsular polysaccharide solution, and then carrying out ultrafiltration and washing on the crude bacterial capsular polysaccharide solution by using a buffer solution with a 30Kd membrane package (Millipore) for 15 sample volumes;
5. the polysaccharide solution was further washed with 10 sample volumes using a 50kD membrane pack (Millipore) and the polysaccharide sample solution was concentrated.
6. The purified polysaccharide solution was collected into lyophilization vials, lyophilized on a vacuum lyophilizer, and stored at-70 ℃.
Embodiment two: preparation of recombinant proteins of herpes zoster virus
Varicella zoster virus (V a r i c e l l a-Z o s t e r V i r u s, VZ V) is a member of the Herpesviridae (Herpesviridae) alpha Herpesviridae subfamily, human herpesvirus 3.
gE (glycine E, gE) is encoded by ORF68 gene, which consists of 1869 bases and is located in a short fragment region of the VZV genome, and the encoded gE contains 623 Amino Acids (AA) with the amino acid sequence shown in SEQ ID No: 1. The N-terminal is 546 AA hydrophilic extracellular regions (containing signal peptide), and the C-terminal is 17AA transmembrane hydrophobic region and 62AA intracellular region. Gene sequence No. GeneID 1487709. The amino acid sequence is shown as SEQ ID No: 2.
Construction of CHO cell expression Strain of varicella zoster Virus gE protein Gene (VZV gE)
1. Synthesis of VZV gE target Gene
The complete gE gene is truncated to remove the transmembrane and intracellular regions, leaving the N-terminal 546 amino acids. The restriction endonuclease EcoRI sequence is added at the C end of the segment gene, the restriction endonuclease XbaI sequence is added at the N end, and the designed nucleotide sequence is synthesized chemically.
2. Plasmid amplification and target gene extraction
The pUC19 plasmid vector is connected with the synthesized gene after double restriction by EcoRI and XbaI, and is led into an amplified host DH5 alpha, and a LB (amp+) agar solid medium is used for screening monoclonal; the monoclonal containing the target gene is inoculated in LB (amp+) liquid culture medium, cultured and amplified at 37 ℃ and 200rpm, and plasmid pUC19-gE is extracted by using a mass preparation kit of Sigma-Aldrich GenEluteTMHP plasmid; the plasmid thus extracted was digested with EcoRI and XbaI restriction enzymes, and the target gene fragment gE was recovered by TaKaRa MiniBest Agarose Gel Extraction Kit.
3. Construction of eukaryotic expression vectors
The mammalian cell expression plasmid pGN-M, which contains the CMV promoter and the dihydrofolate reductase (DHFR) gene, was digested with EcoRI and XbaI restriction enzymes, and the vector DNA fragment was recovered using TaKaRa MiniBEST DNA Fragment Purification Kit Ver.4.0; the vector DNA fragment and the target gene fragment are connected in a tail end sticking mode and are led into a DH5 alpha amplification host, and a monoclonal containing eukaryotic expression plasmid pGN-M_gE is obtained through screening; amplified plasmids were extracted using endotoxin-free plasmid extraction kit TaKaRa MidiBEST Endo-free Plasmid Purification Kit, designated VZVgE, by inoculating to LB (amp+), and performing amplification culture.
Expression of (di) glycoprotein E protein in CHO K1 cells and cloning selection
CHO K1 cells purchased from ATCC were used as host cells, and the cells were recovered and cultured in DMEM medium (Sigma-Aldrich) was passaged 1 every 3 days. After 2 generations, the observed cells grow well, and then CHO K1 cells are cultured according to the ratio of 0.75X10 6 Three 9.6cm2 wells of cells/well were supplemented with Iscove's optimized DMEM medium (Sigma-Aldrich) and 10% fetal bovine serum (IMEM+FBS) (Gibco). Cells were loaded with 4. Mu.g of pcDNAVZVE vector per well in a humidity saturated incubator at 5% CO2 and 37℃and DNA was mixed with Lipofectamine 2000 (Sigma-Aldrich) and added to two of the wells, lipofectamine 2000 alone was added to the third well as a negative control. After 48 hours, the medium was removed and centrifuged at 200 Xg for 5 minutes, and the centrifuged supernatant was stored at-20 ℃. IMDM+FBS medium and 10. Mu.g/mL of Blastidin-HCl (Invitrogen) were added to one well of transfected cells, and the other transfected cell well was washed with PBS, then lysed with 50mM Tris-HCl, pH 8,150mM NaCl,1% (v/v) Triton X-100containing complete,EDTA-free protease inhibitor cocktail (Roche Diagnostics). The lysate was stored at-20℃by centrifugation at 16000 Xg for 10 min at 4 ℃. Western blot was used to detect if recombinant protein gE was present in supernatant and lysate. After 5 days of culture in selective medium, the cells were eluted with trypsin (Invitrogen) and then serially diluted on 9cm Petri dishes to isolate monoclonal cells. In the next 7-11 days, 42 individual clones were picked and transferred to wells on a 96-well plate. Western blot was used to detect culture supernatants and to screen for high expression of VZV gE. Clones secreting the highest amount of VZV gE protein were subjected to the next round of screening, and finally the cells were expanded and 30 new clones were screened for storage.
Selected clones were amplified into three T175 flasks (nes). Trypsin digestion was added, washed with PBS, resuspended in 250mL spin flasks with 100mL ProCHO4 (Lonza) plus 1 XProHT, 4mM L-glutamine and 2% FBS (Lonza). The culture was performed in a humidified incubator at 37℃under 5% CO2 with a stirring speed of 90rpm with the lid slightly opened to ensure air diffusion. The cells were sampled daily, stained with trypan blue (Sigma-Aldrich), counted and passaged every 3-5 days, after a plateau when the viable cell concentration was above 0.3×106 cells/mL and the viable cell count exceeded 90%. When cells adapt and grow well, BFS is gradually removed, at which point cells are considered to be well suited for serum-free suspension growth.
(III) production of VZV gE protein in bioreactor
A3-liter bioreactor was placed with 1.5 liter perfusion culture and a rotary filtration (10 μm) separator. The culture parameters were set as follows: the temperature was controlled at 37℃by a heating blanket, the pH was adjusted to 6.9 by CO2 or 0.3M sodium hydroxide, the stirring speed was 200-300RPM, and dissolved oxygen (dO 2) was adjusted to 40% of saturated air with a maximum flow of a mixture of N2 and O2 at 200 mL/min. The perfusion rate was 0.3 to 0.8V dilution per day, and cells were sampled from the culture fluid daily for cell counting. Trypan blue staining was used and the glucose and lactate concentrations in the supernatant were measured off-line.
A total of 12.5 l of cell-free culture broth was collected, centrifuged at 8000 Xg for 30 min at 4℃and filtered through a 0.45 μm membrane and concentrated by ultrafiltration using a 10kDa membrane pack. Ultrafiltration was performed with buffer, and the sample solution was concentrated to a volume of 0.5 liter, added with 0.5 liter of PBS, and then concentrated to 0.5 liter. The above steps were repeated 5 times.
(IV) bioreactor culture gE protein yield and product Properties
When the cells were inoculated for culture, the cell density was 0.3X106/mL (see FIG. one), followed by culturing in a batch culture mode for 75 hours until the cell density reached 3.5X106/mL. At this stage, the perfusion culture mode was started to perform the culture, initially with a culture volume exchange of 0.3 per day, and then with a 3 per day step up to 0.65 culture volume over the next 120 hours, and maintained between 0.65 and 0.77 per day in the latter period. When the cell density reaches 9X 106/mL, the growth of the cells is stopped rapidly, and the cell activity rate is gradually reduced to 80% from more than 90% in the initial stage.
The protein yield of interest was assessed by density map analysis (image analysis) of stained gE protein on a western blot membrane, then calculated as culture capacity yield, recorded as the time-space yield (mg/L/day) which gradually increased during the culture to 300 hours, followed by the beginning of the decline (see FIG. two) and the pot was stopped after 380 hours of culture.
(fifth) VZV gE protein purification
The sample solution was loaded onto a Q-Sepharose fast flow (GE Bioscience) column and the column was washed with 20mM Tris-HCl pH 7.5. The column was then washed with 20mM Tris-HCl pH7.5, to which 200mM sodium chloride was added, to further remove the adsorbed protein impurities. The gE protein was eluted with a solution that increased the concentration of sodium chloride solution to 300 mM. Ammonium sulfate was added to the pool to a concentration of 800mM, loaded onto Butyl-Sepharose (GE Bioscience) column, the column was washed with phosphate buffer (PBS, 6mM Na2HPO4,1.5mM KH2PO4,0.15M sodium chloride pH 6.8) added 800mM ammonium sulfate, the column was washed with PBS containing 400mM ammonium sulfate, and the gE protein was finally eluted with purified water. Finally loading the sample Sephacryl S-400HR (GE Bioscience), washing the column with PBS, collecting gE protein peak, adding cosolvent, lyophilizing on a vacuum lyophilizing machine, and storing at-70deg.C for use.
Embodiment III: preparation of recombinant protein conjugates of pneumococcal serotypes Pn1, 3, 4, 5, 14, 18C polysaccharide-herpes zoster virus
1) 17.5mg of purified capsular polysaccharide of the corresponding serotype is weighed and dissolved in 4mL of sodium phosphate buffer;
2) 12mg of 1-cyano-4-dimethylammonium pyridine tetrafluoroborate (CDAP) (Sigma-Aldrich) was added to the polysaccharide solution, and the mixture was stirred and reacted at room temperature for 1 hour;
3) Adding 3Eqm cystamine, and reacting for 1 hour at room temperature;
4) Adding 0.3mL of 1M lysine (Sigma-Aldrich) solution for quenching reaction, and reacting for 1-2 hours at room temperature;
5) Adding 8Eqm of 3 (2-chloroethyl) phosphate to the polysaccharide solution to reduce disulfide bonds in the polysaccharide;
6) Transferring the activated polysaccharide solution to a dialysis bag, dialyzing with phosphate buffer solution at 4deg.C, and changing the solution four times;
7) Weighing 30mg of carrier protein to be dissolved in phosphate buffer solution, wherein the protein concentration is 10mg/mL;
8) 8mg of N-hydroxysuccinimide Bromoacetate (BAANS) (Sigma-Aldrich) was added to the carrier protein solution, reacted at room temperature for 2 hours, the activated protein solution was transferred to a dialysis bag (Thermo Scientific), and the solution was dialyzed against phosphate buffer at 4℃for four times;
9) Mixing 4mL of activated polysaccharide solution with 4mL of activated protein solution, and reacting for 4 hours at room temperature;
10 4Eqm N-acetyl-L-cysteine (Sigma-Aldrich) at 2-8deg.C for 4 hours, 12Eqm iodoacetamide (Sigma-Aldrich) at 2-8deg.C for 4 hours;
11 Transferring the polysaccharide conjugate reaction solution to a dialysis bag, and dialyzing the phosphate buffer solution at 4 ℃;
12 Sample solution is loaded with Sepharose CL-4B, purified by the process, and the outer water volume conjugate is collected.
13 Filtering with 0.22 μm filter membrane, and storing at 2-8deg.C.
Embodiment four: preparation of recombinant protein conjugates of pneumococcal serotypes Pn6A, 6B, 7F, 9V, 19A, 19F and 23F polysaccharide-herpes zoster virus
1) Weighing 500mg of purified capsular polysaccharide of the corresponding serotype, and dissolving 500mL in purified water;
2) Degrading by a high-pressure homogenizer at 600bar, adding 0.12eqm sodium periodate (Sigma-Aldrich), and reacting for 18 hours in dark;
3) Ultrafiltration and washing, wherein the molecular weight is 50Kd, the purified water is subjected to ultrafiltration and washing, and concentrated and freeze-dried;
4) Weighing 18mg of activated polysaccharide, adding 4mL of DMSO, and stirring to dissolve completely;
5) 19mg of carrier protein CRM197 was added to 4mL of DMSO (Sigma-Aldrich) solution, 2Eqm sodium cyanoborohydride (Sigma-Aldrich) was added and reacted at room temperature for 22 hours;
6) After 2Eqm sodium borohydride (Sigma-Aldrich) is added to quench the reaction for 4 hours, the synthetic reaction solution is transferred to a dialysis bag, and the buffer solution is dialyzed and changed four times;
7) The sample solution was loaded onto Sepharose CL4B and the conjugate fraction of the external water volume was collected.
8) Filtering with 0.22 μm filter membrane, and storing at 4deg.C.
9) The samples were taken to detect the conjugate molecular weight, polysaccharide protein concentration and ratio.
CRM197 is a preparation method of a conventional polysaccharide protein conjugate with carrier protein, and the protein synthesized conjugate is used as a control for preparing the recombinant protein of the herpes zoster virus as the carrier protein synthesized conjugate. Examples five and six were synthetic conjugates using CRM197 carrier protein.
Fifth embodiment: preparation of pneumococcal serotype Pn1, 3, 4, 5, 14, 18C polysaccharide CRM197 conjugates
1) Weighing 10mg of purified capsular polysaccharide of the corresponding serotype, and dissolving 2mL of the capsular polysaccharide in a sodium phosphate buffer;
2) 10mg of 1-cyano-4-dimethylammonium pyridine tetrafluoroborate (CDAP) (Sigma-Aldrich) was added to the polysaccharide solution, and the mixture was stirred and reacted at room temperature for 1 hour;
3) Adding 3Eqm cystamine, and reacting for 1 hour at room temperature;
4) Adding 0.2mL of 1M lysine solution to perform quenching reaction, and reacting for 1 hour at room temperature;
5) Adding 4Eqm of 3 (2-chloroethyl) phosphate to the polysaccharide solution to reduce disulfide bonds in the polysaccharide;
6) Transferring the activated polysaccharide solution to a dialysis bag, dialyzing with phosphate buffer solution at 4deg.C, and changing the solution four times;
7) Weighing 10mg of carrier protein and dissolving in 1mL of phosphate buffer solution, wherein the protein concentration is 10mg/mL;
8) Adding 3mg of bromoacetic acid N-hydroxysuccinimide ester (BAANS) into the carrier protein solution, reacting for 2 hours at room temperature, transferring the activated protein solution into a dialysis bag, dialyzing the phosphate buffer solution at 4 ℃ and changing the solution four times;
9) Mixing 4mL of activated polysaccharide solution with 4mL of activated protein solution, and reacting for 4 hours at room temperature;
10 2Eqm N-acetyl-L cysteine is added, the reaction is carried out for 4 hours at 4 ℃, 2Eqm iodoacetamide is added, and the reaction is carried out for 4 hours at 4 ℃;
11 Transferring the polysaccharide conjugate reaction solution to a dialysis bag, and dialyzing the phosphate buffer solution at 4 ℃;
12 Sample solution is loaded with Sepharose CL-4B, purified by the process, and the outer water volume conjugate is collected.
13 After filtration through a 0.22 μm filter, the preparation is stored at 4 ℃.
Example six: preparation of pneumococcal serotype Pn6A, 6B, 7F, 9V, 19A, 19F and 23F polysaccharide CRM197 conjugates
1) Weighing 500mg of purified capsular polysaccharide of the corresponding serotype, and dissolving 500mL in purified water;
2) Degrading by a high-pressure homogenizer at 600bar for three times, adding 0.3Eqm sodium periodate, and reacting for 18 hours in dark place;
3) Ultrafiltration and washing, wherein the molecular weight is 50Kd, the purified water is subjected to ultrafiltration and washing, and concentrated and freeze-dried;
4) Weighing 10mg of activated polysaccharide, adding 2mL of DMSO, and stirring to dissolve completely;
5) 2mL of a DMSO solution of 10mg of carrier protein CRM197 was added, and 3eqm of sodium cyanoborohydride was added to react at room temperature for 22 hours;
6) Adding 1Eqm sodium borohydride to quench for 4 hours, transferring the synthetic reaction solution to a dialysis bag, dialyzing the buffer solution, and changing the solution four times;
7) The sample solution was loaded onto Sepharose CL4B and the conjugate fraction of the external water volume was collected.
8) Filtering with 0.22 μm filter membrane, and storing at 4deg.C.
9) The samples were taken to detect the conjugate molecular weight, polysaccharide protein concentration and ratio.
EXAMPLE seven, 13 valent pneumococcal polysaccharide-recombinant protein conjugate vaccine of herpes zoster Virus+CpG+Alum (formulation A)
Measuring polysaccharide concentration in monovalent conjugate, measuring polysaccharide amount corresponding to 2.2 μg (except Pn6B polysaccharide amount 4.4 μg) respectively, and sterilizing Pn1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F into sterile container, sampling protein content 39.2 μg, adding CpG (Genscript), adding phosphate buffer pH 5.8 buffer, and sterilizing with 0.22 μm membrane, and filtering; adding sterile aluminum phosphate gel with final aluminum ion content of 0.125mg, stirring at 4deg.C for 1 hr, aseptically packaging into 0.5 mL/bottle, and preserving at 4deg.C for immunization.
Example eight: pneumococcal polysaccharide 13-CRM 197 conjugate vaccine +CpG +Alum (preparation B)
Measuring polysaccharide concentration in monovalent conjugate, measuring polysaccharide amount (except Pn6B polysaccharide amount of 4.4 μg) corresponding to 2.2 μg respectively from CRM197 protein conjugate solution (see examples five, and six) including Pn1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F and 23F into sterile container, sampling to detect protein content of 41.4 μg, adding CpG, adding final amount of 0.1mg, adding phosphate buffer pH5.8 buffer, sterilizing with 0.22 μm membrane, and filtering; adding sterile aluminum phosphate gel (Bennetag) with final aluminum ion content of 0.125mg, stirring at 4deg.C for 1 hr, aseptically packaging into 0.5 mL/bottle, and preserving at 4deg.C for immunization.
Example nine: preparation immune rabbit and blood sampling
Taking 10 or 5 groups of 2.5-3.5 kg New Zealand white rabbits, wherein one group of the immune preparation A, another group of immunization preparation B, each rabbit was injected 0.5 mL/time, divided into 0 weeks, and immunized once every 2 weeks; the blood sampling time is 0 weeks before immunization, 1 week after the second immunization, and three times. One part of the blood was prepared into PBMC, quick frozen on dry ice and then stored at low temperature, the other part was left at room temperature for 4 hours, centrifuged at 10000RPM at room temperature, and the supernatant serum was aspirated off the heart and stored at-70℃to be tested.
Example ten: ELISA (enzyme-Linked immunosorbent assay) for detecting polysaccharide antibody titer in rabbit immune serum of 13-valent pneumococcal polysaccharide-gE conjugate vaccine
Pneumococcal polysaccharides of different serotypes (1 XPBS solution) were separately prepared and stored in a refrigerator at 4 ℃. The ELISA plates were coated by diluting the serotype pneumococcal polysaccharide to be tested at 4. Mu.g/mL and adding 100. Mu.L of coating solution to each well and incubated overnight at room temperature. Washing with plate washing buffer 4 times, adding 100 μl of blocking buffer, incubating at room temperature for 2 hours, washing with plate washing buffer 4 times, and storing at 4deg.C for one week.
The corresponding serum to be tested obtained by injecting the vaccine and the control sample into the rabbit is diluted 1:10 into the serum of the working sample, the serum is diluted by proper times, the serum is added into the first row of holes of the ELISA plate, the total volume is 200 mu L, the serial dilution is performed downwards from the first row, and the serum is incubated for 2 hours at room temperature. Washing with plate buffer solution for 4 times, adding 100. Mu.L alkaline phosphatase labeled goat anti-rabbit antibody (1:2000 dilution), and incubating for 4 hours at room temperature. Wash with plate buffer 4 times, add 100. Mu.L of 4-nitrophenyl phosphate disodium salt substrate (Sigma-Aldrich) solution and read the plate at 405 nm.
Table 1: preparation A and preparation B detection results of IgG antibody titer of pneumopolysaccharide and recombinant protein of herpes zoster virus in antiserum after rabbits are immunized
Formulation a and formulation B differ in that the carrier protein, the method of synthesis and the adjuvant are essentially the same. Polysaccharide and carrier protein antibody IgG detection results show that before immunization, the polysaccharide antibody titer of each serotype has no significant difference, and after immunization of a first needle, preparation A (herpes zoster recombinant protein carrier) and preparation B (CRM 197 protein carrier) stimulate animals to generate corresponding serotype polysaccharide antibody IgG titer which is different according to serotypes, for example, the results of serotypes 1, 3, 4, 6B, 18C, 19A and 23F show that the preparation A is higher than the preparation B and accounts for 54 percent of components; serotypes 6A, 14, and 19F were not significantly different, accounting for 23% of the components; serotypes 5 and 7F, formulation B were slightly higher than formulation a, accounting for 15%. Thus, the preparation A prepared by taking the recombinant protein of the herpes zoster virus as the carrier protein is superior to the preparation B prepared by taking CRM197 as the carrier protein. After immunization of two needles, the antibody IgG detection results showed more significant differences between preparation A and preparation B, and serotypes 1, 3, 4, 5, 6A, 7F, 9V, 14, and 18C showed that preparation A was higher than preparation B, accounting for 69% of the components, no significant differences between serotypes 6B, 19A, 19F, accounting for 23% of the components; serotype 23F assay showed that formulation B was slightly higher than formulation a, accounting for 7.7% of the component. Thus, the recombinant herpes zoster protein is used as carrier protein, and has better antigenicity enhancing effect on polysaccharide.
For the detection result of the antibody titer of the recombinant protein of the herpes zoster, the antibody titer is obviously enhanced along with the increase of the immunization times, and the titer after the immunization of the second needle is obviously higher than the IgG titer after the immunization of the first needle.
Example eleven: recombinant proteins of herpes zoster and multivalent pneumococcal polysaccharide-herpes zoster virus recombinant protein conjugate for immunizing mice, and blood sampling and spleen collection
Female BALB/c mice of 6 weeks are taken and randomly grouped, 8 mice in each group are immunized subcutaneously once every two weeks, 0.1mL of mice are immunized twice, blood is collected after one week of immunization, and specific vaccine types and dosage information are as follows:
embodiment twelve: ELISA (enzyme-Linked immuno sorbent assay) for detecting gE protein antibody titer in recombinant protein gE of herpes zoster virus and gE protein antibody titer in mouse immune serum of 13-valent pneumococcal polysaccharide-gE combined vaccine
Purified gE protein stock solution 1mg/mL (1 XPBS solution) was prepared and stored in a refrigerator at 4 ℃. The ELISA plates were coated by diluting the gE protein stock to 4. Mu.g/mL of coating buffer, adding 100. Mu.L of coating solution to each well, and incubating overnight at room temperature. Washing with plate washing buffer 4 times, adding 100 μl of blocking buffer, incubating at room temperature for 2 hours, washing with plate washing buffer 4 times, and storing at 4deg.C for one week.
The corresponding serum to be tested obtained by injecting the vaccine and the control sample into the mice is diluted 1:10 into the serum of the working sample, the serum is diluted by proper times, the serum is added into the first row of holes of the ELISA plate, the total volume is 200 mu L, the serial dilution is performed downwards from the first row, and the serum is incubated for 2 hours at room temperature. Washing with plate buffer solution for 4 times, adding 100. Mu.L alkaline phosphatase labeled goat anti-mouse antibody (1:2000 dilution), and incubating for 4 hours at room temperature. The plate was washed 4 times with plate washing buffer, 100. Mu.L of 4-nitrophenyl phosphate disodium salt substrate solution was added and the plate read at 405 nm.
Table 2: geometric mean (Eu) of anti-gE IgG antibody titers in different vaccine immune antisera
The detection result shows that if no adjuvant is added in the vaccine preparation, the recombinant protein gE of the herpes zoster has low antigenicity and cannot be prepared into a vaccine. And after the adjuvant CpG+Alum is added, antigenicity is obviously improved, and the total titer of IgG, igG1 and IgG2a is improved. The results also show that, in multivalent pneumococcal polysaccharide-gE protein conjugate vaccine, the total content of gE protein is 39.3 mug, which is significantly higher than that of conventional recombinant herpes zoster virus protein vaccine, wherein the amount of gE protein used for immunizing mice is 5 mug, therefore, gE antibodies in immune serum of the 13-valent polysaccharide-gE conjugate vaccine, including total IgG, igG1 and IgG2a, are about twice higher than that of gE vaccine. When the 13-valent polysaccharide-gE conjugate vaccine was diluted to a protein concentration of 5 μg, the antibody titer was lower than that of the gE vaccine, due to loss of protein surface antigen clusters during conjugate synthesis of gE protein.
Embodiment thirteen: IFN-gamma ELISpot and cytokine detection
The experimental mice of example eleven were collected with spleen collected, after grinding the spleen, added to PBS pH7.4, suspended, subjected to gradient dilution, and the single cell suspension obtained was added to an ELISPot plate, on which a capture antibody was pre-coated. Add 5. Mu.g/mL gE protein, VZV (100 pfu/mL), concanavalin A (1. Mu.g/mL, positive control) (Sigma-Aldrich) and incubate at 37℃under 5% CO2 for 24 hours. Cells were detected on IFN-gamma using ELISPot kit (MabTech) and positive spots were determined using CTL-ImmunoSpot S5UV Micro analyzer.
Table 3: IFN-gamma SFC/10 in spleen cells of mice immunized with different vaccines 6 Cell number
Vaccines with adjuvant components, such as adjuvant-containing gE, adjuvant-containing 13-valent pneumococcal polysaccharide-gE combined vaccine and adjuvant-containing 13-valent polysaccharide-gE combined vaccine diluent, have the effect of stimulating immune cells in spleen of an object, such as NK cells, phagocytes and the like, and produce IFN-gamma. The detection result shows that the adjuvant-containing 13-valent pneumopolysaccharide-gE combined vaccine is sensitized by immune cells to generate IFN-gamma under the stimulation of gE antigen or virus; IFN-gamma produced by immune cells sensitized by the 13-valent pneumococcal polysaccharide-gE combined vaccine is obviously higher than that of the gE vaccine containing the adjuvant; for the same reason, spleen immune cells sensitized with adjuvant-containing conjugate vaccine dilutions of pneumococcal polysaccharide-gE at 13-valent produced lower amounts of IFN- γ.
The foregoing is merely exemplary embodiments of the present invention and are not intended to limit the scope of the present invention, and all equivalent modifications made by the present invention or direct or indirect application in other related technical fields are included in the scope of the present invention.
Claims (12)
1. The pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine with immunogenicity is characterized in that the protein in the pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein combined vaccine is the herpes zoster virus recombinant glycoprotein with immunogenicity;
The pneumococcal capsular polysaccharide is connected with the herpes zoster virus recombinant protein through a covalent bond;
the recombinant proteins of the herpes zoster virus are selected from gE glycoprotein, and the amino acid sequence of the recombinant proteins is shown as SEQ ID No:1 or SEQ ID No:2, said pneumococcal capsular polysaccharide is selected from at least one of serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F.
2. The immunogenic pneumococcal capsular polysaccharide-herpes zoster virus recombinant protein conjugate vaccine of claim 1, wherein the conjugate vaccine is in the form of an aqueous solution or a lyophilized preparation.
3. The recombinant protein vaccine of pneumococcal capsular polysaccharide-herpes zoster virus with immunogenicity according to claim 2, wherein the conjugate vaccine comprises an adjuvant.
4. The immunogenic recombinant pneumococcal capsular polysaccharide-herpes zoster virus protein conjugate vaccine of claim 3, wherein the adjuvant is one of CpG, QS21, aluminum phosphate, a mixture of CpG and aluminum phosphate, or a mixture of QS21 and aluminum phosphate.
5. A nucleotide sequence encoding the recombinant protein of the herpes zoster virus of claim 1.
6. A recombinant expression vector comprising the nucleotide sequence of claim 5.
7. The method for preparing the recombinant protein of the herpes zoster virus by using the recombinant expression vector as claimed in claim 6, wherein the expression method adopts a CHO cell expression system or an insect baculovirus expression system.
8. The method for preparing the recombinant protein conjugate vaccine of pneumococcal capsular polysaccharide and herpes zoster virus with immunogenicity according to claims 1 to 4, wherein the recombinant protein conjugate of pneumococcal capsular polysaccharide and herpes zoster virus is obtained by chemical synthesis reaction in buffer or organic solvent.
9. The method according to claim 8, wherein the organic solvent is selected from dimethyl sulfoxide and dimethylformamide.
10. The method according to claim 8, wherein the chemical synthesis reaction is selected from one of a reductive amine method, a 1-cyano-4-dimethylamino pyridinium tetrafluoroborate method, an adipoyl dihydrazide method and a 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride method.
11. The method of claim 8, further comprising pretreatment of pneumococcal capsular polysaccharide, expression and purification of recombinant proteins of herpes zoster virus, and purification of conjugates.
12. The method of claim 11, wherein the pretreatment of the polysaccharide comprises degradation and activation, and wherein the degradation method is selected from the group consisting of high pressure homogenizer degradation, acid hydrolysis, and enzymatic digestion.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785857A (en) * | 2010-03-05 | 2010-07-28 | 郑玉清 | Novel pneumococcal conjugate vaccine and preparation method thereof |
| CN101972475A (en) * | 2010-04-12 | 2011-02-16 | 李建平 | Bacterial polysaccharide-protein conjugate vaccine and preparation method thereof |
| CN103656631A (en) * | 2012-09-24 | 2014-03-26 | 北京科兴中维生物技术有限公司 | Multivalence pneumococcus capsular polysaccharide-protein conjugate composition and preparation method thereof |
| WO2015115767A1 (en) * | 2014-01-28 | 2015-08-06 | 연세대학교 원주산학협력단 | Method for diagnosing immunity against varicella zoster virus using human cell expressing glycoprotein e of varicella zoster virus |
| WO2015144031A1 (en) * | 2014-03-26 | 2015-10-01 | 天津康希诺生物技术有限公司 | Pneumococcus polysaccharide protein conjugated vaccine and preparation method therefor |
| CN109602901A (en) * | 2019-01-08 | 2019-04-12 | 成都迈科康生物科技有限公司 | A kind of herpes zoster virus vaccine and its preparation method and application |
| CN113683704A (en) * | 2021-07-28 | 2021-11-23 | 安徽智飞龙科马生物制药有限公司 | Varicella-zoster virus r-gE fusion protein, recombinant varicella-zoster vaccine, and preparation method and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0428394D0 (en) * | 2004-12-24 | 2005-02-02 | Chiron Srl | Saccharide conjugate vaccines |
| KR102098097B1 (en) * | 2017-08-16 | 2020-05-26 | 주식회사 차백신연구소 | Vaccine adjuvant comprising lipopeptide-inserted liposome as an effective ingredient and using thereof |
-
2022
- 2022-10-28 CN CN202211335608.0A patent/CN115721711B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785857A (en) * | 2010-03-05 | 2010-07-28 | 郑玉清 | Novel pneumococcal conjugate vaccine and preparation method thereof |
| CN101972475A (en) * | 2010-04-12 | 2011-02-16 | 李建平 | Bacterial polysaccharide-protein conjugate vaccine and preparation method thereof |
| CN103656631A (en) * | 2012-09-24 | 2014-03-26 | 北京科兴中维生物技术有限公司 | Multivalence pneumococcus capsular polysaccharide-protein conjugate composition and preparation method thereof |
| WO2015115767A1 (en) * | 2014-01-28 | 2015-08-06 | 연세대학교 원주산학협력단 | Method for diagnosing immunity against varicella zoster virus using human cell expressing glycoprotein e of varicella zoster virus |
| WO2015144031A1 (en) * | 2014-03-26 | 2015-10-01 | 天津康希诺生物技术有限公司 | Pneumococcus polysaccharide protein conjugated vaccine and preparation method therefor |
| CN109602901A (en) * | 2019-01-08 | 2019-04-12 | 成都迈科康生物科技有限公司 | A kind of herpes zoster virus vaccine and its preparation method and application |
| CN113683704A (en) * | 2021-07-28 | 2021-11-23 | 安徽智飞龙科马生物制药有限公司 | Varicella-zoster virus r-gE fusion protein, recombinant varicella-zoster vaccine, and preparation method and application thereof |
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