CN115718095B

CN115718095B - Automatic scanning method and device for circulating tumor cells

Info

Publication number: CN115718095B
Application number: CN202210313852.0A
Authority: CN
Inventors: 蔡惠明; 钱露; 卢露
Original assignee: Nanjing Nuoyuan Medical Devices Co Ltd
Current assignee: Nanjing Nuoyuan Medical Devices Co Ltd
Priority date: 2022-03-28
Filing date: 2022-03-28
Publication date: 2023-09-01
Anticipated expiration: 2042-03-28
Also published as: CN115718095A

Abstract

The invention provides an automatic scanning method and device for circulating tumor cells, which are characterized in that a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number are generated by carrying out fluorescent scanning on a culture solution with a blood sample at fixed time, and a first scanning moment, a second scanning moment and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number are recorded; generating a first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time; comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data; the invention can accurately predict the growth trend of the circulating tumor cells.

Description

Automatic scanning method and device for circulating tumor cells

Technical Field

The invention relates to a data processing technology, in particular to an automatic scanning method and device for circulating tumor cells.

Background

The circulating tumor cells (circulating tumor cell) are called CTC (tumor cells) for short, and the tumor cells entering human peripheral blood are usually called circulating tumor cells, and at present, whether the individual tumor values are normal or the postoperative effect of a patient is judged mainly by continuously acquiring the values of the circulating tumor cells manually, so that the method is used for monitoring the tumor conditions in real time and evaluating the treatment effect, and real-time individual treatment is realized.

In the prior art, the judgment standard of a doctor on the tumor condition is generally data of dimensions such as the type, the number, the diffusion area, the growth trend and the like of the circulating tumor cells, wherein the judgment on the growth trend of the circulating tumor cells is generally carried out by inquiring some information of a patient, such as when symptoms start to exist, and then combining personal experience to judge the growth trend, so that the accuracy is low.

Therefore, how to predict the growth trend of circulating tumor cells more accurately is an urgent problem to be solved.

Disclosure of Invention

The embodiment of the invention provides an automatic scanning method and device for circulating tumor cells, which can be used for accurately predicting the growth trend of the circulating tumor cells.

In a first aspect of the embodiment of the present invention, there is provided a method for automatically scanning circulating tumor cells, including a camera, a timing device and a processor, the processor automatically scanning the circulating tumor cells by:

Performing fluorescent scanning on the culture solution with the blood sample at fixed time to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording a first scanning moment, a second scanning moment and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number;

generating a first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time;

and comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data.

Optionally, in a possible implementation manner of the first aspect, the step of performing fluorescent scanning on the culture solution with the blood sample placed thereon at a timing to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number, and a third measured fluorescent tumor number, and recording a first scanning moment, a second scanning moment, and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number, and the third measured fluorescent tumor number specifically includes:

Scanning the culture solution by an automatic scanner within a preset time period, detecting the number of tumors by the brightness of the fluorescent pixel value to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording first scanning time, second scanning time and third scanning time corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number;

acquiring a luminescence curve of a fluorescence detection agent used in the detection of the circulating tumor cells;

according to a preset scanning moment corresponding to the maximum value of the luminous efficiency in the luminous curve, aligning the first scanning moment, the second scanning moment and the third scanning moment with luminous efficiency values corresponding to the preset scanning moment;

and performing fluorescence compensation on the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number according to the luminous efficiency difference value after the alignment treatment to obtain a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number and a compensated third measured fluorescent tumor number.

Optionally, in one possible implementation manner of the first aspect, in the step of performing alignment processing on the luminous efficiency values corresponding to the first scanning time, the second scanning time and the third scanning time and the preset scanning time according to the preset scanning time corresponding to the maximum value of the luminous efficiency in the luminous curve, the method specifically includes:

Respectively acquiring a first time difference value, a second time difference value and a third time difference value of a first scanning time, a second scanning time and a third scanning time and a preset scanning time;

and carrying out alignment processing on the luminous efficiency values of the first scanning moment, the second scanning moment and the third scanning moment according to the first moment difference value, the second moment difference value and the third moment difference value.

Optionally, in one possible implementation manner of the first aspect, in performing fluorescence compensation on the first measured fluorescent tumor number, the second measured fluorescent tumor number, and the third measured fluorescent tumor number according to the difference value of luminous efficiency after the alignment treatment, to obtain a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number, and a compensated third measured fluorescent tumor number, according to the step after the alignment treatment, specifically includes:

obtaining a first luminous efficiency difference value, a second luminous efficiency difference value and a third luminous efficiency difference value after alignment processing according to a curve function of a luminous curve by using the first moment difference value, the second moment difference value and the third moment difference value;

generating a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number and a compensated third measured fluorescent tumor number according to the first luminous efficiency difference, the second luminous efficiency difference and the third luminous efficiency difference and the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number;

Calculating the number of the compensated first measured fluorescent tumors, the number of the compensated second measured fluorescent tumors and the number of the compensated third measured fluorescent tumors by the following formula,

wherein ,S₁₁ For the compensated first measurement of the number of fluorescent tumors, S ₂₂ For the compensated second measurement of the number of fluorescent tumors, S ₃₃ For the compensated third measurement of the number of fluorescent tumors, S ₁ First measurement of the fluorescent tumor quantity for the fluorescent scanner measurement S ₂ Second measurement of the fluorescent tumor quantity for the fluorescent scanner measurement S ₃ Third measurement of fluorescent tumor number measured for fluorescent scanner, beta is constant, k ₁ For the first compensation weight, k ₂ For the second compensation weight, k ₃ For the third compensation weight, T ₁ For the first scanning time, T ₂ For the second scanning time, T ₃ For the third scanning time, |T ₁ -t| is the difference between the first scanning instant and the preset scanning instant, |t ₂ -t| is the difference between the second scanning instant and the preset scanning instant, |t ₃ -T| is the third scanning instant and the preset scanning instantDifference value.

Optionally, in a possible implementation manner of the first aspect, the step of generating the first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating the second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time specifically includes:

Generating a compensated first tumor change value by using the compensated first measured fluorescent tumor number, the compensated second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a compensated second tumor change value according to the compensated second measured fluorescent tumor number, the compensated third measured fluorescent tumor number, the second scanning time and the third scanning time;

generating a compensated first tumor change value by using the compensated first measured fluorescent tumor number, the compensated second measured fluorescent tumor number, the first scanning time and the second scanning time based on the Kramer rule;

generating a compensated second tumor change value by using the compensated second measured fluorescent tumor number, the compensated third measured fluorescent tumor number, the second scanning time and the third scanning time based on the Kramer rule;

the first tumor change value and the second tumor change value are calculated by the following formulas,

wherein ,a₁ For the first tumor change value, a ₂ For the second tumor change value, n ₁ For the first variation weight, n ₂ Is the second variation weight.

Optionally, in one possible implementation manner of the first aspect, the tumor change value is corrected according to the physical examination index to obtain a corrected tumor change value, and the first change weight and the second change weight are corrected according to the tumor change value before correction and the tumor change value after correction;

The modified change adjustment weight is obtained by the following formula,

wherein ,n₃ Adjusting weights for the corrected first variation, n ₄ For the second modified change, the weight is adjusted, p is the weight adjustment correction value, N is the normal index number of physical examination, u _i Is the value of the i-th normal index, v _i Is the weight of the i-th normal index, gamma and delta are constants, n is the number of abnormal indexes of physical examination, and x _i Is the value of the i-th abnormal index, y _i Weight, x, of class i anomaly index _i * _i For the degree of influence of the i-th abnormality index, u _i * _i Is the influence degree of the i-th normal index.

Optionally, in a possible implementation manner of the first aspect, in the step of comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data, the method specifically includes:

generating a change value difference value by differencing the first tumor change value and the second tumor change value, and taking the larger value of the first tumor change value and the second tumor change value as a target tumor change value;

if the variation value difference is not in the preset difference value variation value interval, judging that the detection is abnormal, and entering a reinspection flow;

and if the change value difference is in a preset difference value change value interval, comparing the target tumor change value with a first preset change value to obtain a comparison result, and judging whether tumor trend data are normal or not according to the comparison result.

Optionally, in one possible implementation manner of the first aspect, the patient culture fluid is classified according to the second preset change value, the target tumor change value and the first preset change value actively set by the hospital, and the classification result is sent to a corresponding storage area in the server;

if the target tumor change value is smaller than a first preset change value, marking the detection result of the culture solution as no tumor risk, and distributing the detection result to a first storage area in a server according to the marking, wherein the first storage area is a physical examination center calling area;

if the target tumor change value is larger than the first preset change value and smaller than the second preset change value, marking the detection result of the culture solution as moderate tumor risk, and distributing the detection result to a second storage area in the server according to the marking, wherein the second storage area is a common clinic calling area;

if the target tumor change value is larger than the second preset change value, marking the detection result of the culture solution as heavy tumor risk, and distributing the detection result to a third storage area in the server according to the mark, wherein the third storage area is an expert outpatient call area;

monitoring the behaviors of a doctor to obtain doctor behavior data;

and if the doctor behavior data is judged to be any one of the tumor risk-free, moderate tumor risk and severe tumor risk, adjusting at least one of the first preset change value and the second preset change value.

In a second aspect of the embodiments of the present invention, there is provided an automatic scanning device for circulating tumor cells, including:

the tumor number generation module is used for carrying out fluorescent scanning on the culture solution with the blood sample at fixed time to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording a first scanning moment, a second scanning moment and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number;

the tumor change generation module is used for generating a first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time;

and the tumor change comparison module is used for comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data.

In a third aspect of an embodiment of the present invention, there is provided an electronic device including: a memory, a processor and a computer program stored in the memory, the processor running the computer program to perform the first aspect of the invention and the methods that the first aspect may relate to.

A fourth aspect of an embodiment of the present invention provides a readable storage medium, wherein a computer program is stored in the readable storage medium, and the computer program is used for implementing the first aspect of the present invention and the methods possibly related to the first aspect when the computer program is executed by a processor.

The automatic scanning method and the automatic scanning device for the circulating tumor cells can be used for scanning the blood sample in the culture solution at regular time, the number of the circulating tumor cells and the corresponding recorded time are displayed through fluorescence, the growth change value of the circulating tumor cells is obtained, and a doctor is assisted to accurately predict future health conditions of the physical examination person according to the growth change value of the circulating tumor cells.

When the tumor change value is calculated, the invention can perform alignment compensation according to the metabolism condition of human cells on the fluorescence detection agent and according to the time corresponding to the fluorescence luminous efficiency value and the maximum fluorescence luminous efficiency value at the corresponding time, so that the first measured fluorescence tumor number, the second measured fluorescence tumor number and the third measured fluorescence tumor number at the beginning are more objective and accurate.

According to the technical scheme, the first tumor change value and the second tumor change value are subjected to difference to calculate the error between the first tumor change value and the second tumor change value, the error is compared with a preset error interval to judge whether abnormality is detected, if the detection is judged to be normal in the preset difference change value interval, the first tumor change value or the second tumor change value is compared with the first preset change value, if the detection is larger than the first preset change value, the tumor trend data is abnormal, if the detection is smaller than the first preset change value, the tumor trend data is normal, the future development trend of tumors can be judged according to the corresponding value, the illness probability is estimated, and corresponding medical advice is provided.

When calculating the tumor change value, the health degree can influence the tumor change value due to different human body textures, and the change weight of the change value can be adjusted based on the health degree of different people, so that the subsequently output tumor change value is more accurate, and the numerical value has better reference.

According to the technical scheme, the physical examination report is stored in a partitioning mode according to the second preset change value actively set by the hospital so as to be convenient for the follow-up corresponding doctor to call and inform, the physical examination report is stored in a classifying mode according to the marking result, the follow-up doctor can be conveniently called and data confusion can be prevented, and the follow-up detection can be carried out in time according to the numerical value of the patient so as to prevent the disease from being prolonged and deteriorated.

Drawings

FIG. 1 is a schematic diagram of an application scenario of an automatic scanning method for circulating tumor cells;

FIG. 2 is a flow chart of a first embodiment of an automated scanning method for circulating tumor cells;

FIG. 3 is a flow chart of a second embodiment of an automated scanning method for circulating tumor cells;

FIG. 4 is a schematic diagram of a server distribution scenario of the method for automatically scanning circulating tumor cells;

FIG. 5 is a schematic diagram of the structure of an automatic scanning device for circulating tumor cells;

fig. 6 is a schematic structural diagram of an electronic device.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The terms "first," "second," "third," "fourth" and the like in the description and in the claims and in the above drawings, if any, are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the invention described herein may be implemented in sequences other than those illustrated or otherwise described herein.

It should be understood that, in various embodiments of the present invention, the sequence number of each process does not mean that the execution sequence of each process should be determined by its functions and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the present invention.

It should be understood that in the present invention, "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements that are expressly listed or inherent to such process, method, article, or apparatus.

It should be understood that in the present invention, "plurality" means two or more. "and/or" is merely an association relationship describing an association object, meaning that there may be three relationships, e.g., a and/or B, may represent: a exists alone, A and B exist together, and B exists alone. The character "/" generally indicates that the context-dependent object is an "or" relationship. "comprising A, B and C", "comprising A, B, C" means that all three of A, B, C comprise, "comprising A, B or C" means that one of the three comprises A, B, C, and "comprising A, B and/or C" means that any 1 or any 2 or 3 of the three comprises A, B, C.

It should be understood that in the present invention, "B corresponding to a", "a corresponding to B", or "B corresponding to a" means that B is associated with a, from which B can be determined. Determining B from a does not mean determining B from a alone, but may also determine B from a and/or other information. The matching of A and B is that the similarity of A and B is larger than or equal to a preset threshold value.

As used herein, "if" may be interpreted as "at … …" or "at … …" or "in response to a determination" or "in response to detection" depending on the context.

The technical scheme of the invention is described in detail below by specific examples. The following embodiments may be combined with each other, and some embodiments may not be repeated for the same or similar concepts or processes.

As shown in fig. 1, in the application scenario of the technical scheme provided by the invention, a machine places a blood sample in a culture solution, a fluorescent scanning device automatically scans the culture solution placed with the blood sample according to preset time by a timing device, the fluorescent scanning device scans the culture solution respectively to generate fluorescent tumor numbers at corresponding time, a processor generates tumor change values according to the corresponding time and the fluorescent tumor numbers, and the tumor change values are compared with the preset change values to obtain the development trend of tumor data.

The fluorescence scanning device and the timing device are connected with the processor, the processor is connected with the input equipment, a doctor can input other physical examination index values of the physical examination object through the input equipment, and the development trend of tumor data which is more in line with the physical examination object is obtained through comprehensive treatment of other physical examination indexes and tumor change values, so that the result is more accurate and is more in line with the actual situation.

According to the technical scheme provided by the invention, the tumor growth trend of the physical examination object can be obtained according to the time and the corresponding tumor number.

The invention provides an automatic scanning method of circulating tumor cells, which is shown in fig. 3, and comprises the following steps of S110-S130:

step S110, performing fluorescence scanning on the culture solution with the blood sample at fixed time to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording a first scanning moment, a second scanning moment and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number.

According to the technical scheme provided by the invention, a physical examination object firstly takes an action medicament containing a fluorescent agent, a corresponding blood sample is obtained in the action time of the medicament and is put into a culture solution, the culture solution is scanned in a time period with a larger slope of a luminous curve of a corresponding fluorescent detection agent by a fluorescent scanner, a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number are sequentially generated after scanning, corresponding first scanning time, second scanning time and third scanning time are recorded, a subsequent tumor change value is generated according to the obtained tumor number and time, wherein if the time with the slope of the corresponding luminous curve peak of the fluorescent detection agent being not 0 is longer, multiple detection can be performed to generate multiple change values for verification, and the method is not limited.

In a possible implementation manner, the step 110 includes steps S1101 to S1103, which are specifically as follows:

step 1101, scanning the culture solution by an automatic scanner within a preset time period, detecting the number of tumors by the brightness of the fluorescent pixel value to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording a first scanning time, a second scanning time and a third scanning time corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number.

According to the technical scheme provided by the invention, as the human body has the circulating tumor cells, the immune system of the human body can be killed only when the circulating tumor cells enter the blood, for example, the circulating tumor cells can be phagocytized by macrophages after entering the blood, and even some circulating tumor cells can be apoptosis due to external extrusion, so that the corresponding quantity of the circulating tumor cells is detected by fluorescence, and preparation is made for obtaining the growing trend and the preset normal trend subsequently; estimating the luminescence curve of the fluorescent detection agent based on the metabolic efficiency of the reagent by conventional cells A period in which the slope is not 0 in the peak period, for example: the time interval corresponding to one half of the maximum value of the luminous efficiency can be selected as a preset time interval, at this time, the fluorescent scanning device scans the culture solution for 3 times to scan out tumor cells acted by the fluorescent detection agent, and due to the infinite division property of tumor cells, nutrition in the culture solution can be infinitely increased only by sufficient nutrition, so that the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number are respectively generated and recorded through 3 times of pre-scanning, and the first scanning moment, the second scanning moment and the third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number are recorded, for example: the automatic scanning device scans for the first time at the 1.3-month and 1-day time at the 12:00:00.m. and scans for the fluorescent tumors 50 in number, the automatic scanning device scans for the second time at the 1.3-month and 1-day time at the 12:30:03.m. and scans for the fluorescent tumors 100 in number, the automatic scanning device scans for the third time at the 1.3-month and 1-day time at the 13:00:03.m. and scans for the fluorescent tumors 200 in number, wherein the first scanning time T ₁ =12×3600+0×60+0, second scan time T ₂ =12×3600+30×60+3, third scan time T ₃ =13×3600+0×60+3; it can be appreciated that the first scanning time T corresponds to ₁ = 43200 seconds, T ₂ =45003 seconds, T ₃ = 46803 seconds.

Step S1102, obtaining a luminescence curve of a fluorescent detection agent used in detection of the circulating tumor cells.

According to the technical scheme provided by the invention, the fluorescence light-emitting curve corresponding to the used fluorescence detection agent is obtained, the light-emitting efficiency corresponding to each moment is known through the fluorescence curve, the fluorescent quantity of the tumor is conveniently compensated according to the difference between the obtained current moment and the highest light-emitting efficiency, so that the obtained fluorescent quantity is more accurate, the obtained tumor change value is more accurate, the tumor change value is the change value of the tumor quantity, the tumor change value can be used for representing the future growth trend of the tumor, the ideal fluorescence light-emitting curve is gradually improved from the gradual start, the metabolism of the subsequent cells is gradually weakened, and finally certain residues remain, and the tumor change value can be seen as being close to a normal distribution map.

Step S1103, performing alignment processing on the luminous efficiency values corresponding to the first, second and third scanning moments and the preset scanning moment according to the preset scanning moment corresponding to the maximum value of the luminous efficiency in the luminous curve.

According to the technical scheme provided by the invention, in daily detection, metabolism of cells is usually ignored, the attenuation degree of luminescence of corresponding detection reagents is increased, if the detection time difference is too large, the corresponding error is increased further, the subsequent detection result is inaccurate, the time corresponding to the maximum value of the luminescence efficiency is obtained according to the fluorescence luminescence curve corresponding to the fluorescence detection reagents, and the luminescence efficiency corresponding to the first scanning time, the second scanning time and the third scanning time is aligned with the maximum value of the luminescence efficiency, for example: after the fluorescence detection agent enters the cells, a slow action process is carried out, the luminous efficiency corresponding to the increase of time gradually increases, the luminous efficiency corresponding to the increase of time gradually declines due to the metabolism of the cells after the maximum value is reached, and then the slope is almost consumed and approaches to 0, and as can be understood, the step is as good as possible when the slope is not 0 and the absolute value of the slope is larger; for example, a time period corresponding to a position where the maximum luminous efficiency of the fluorescence light-emitting curve is half can be preset and selected as a time period of 3 times of automatic scanning, so that the corresponding error is smaller, the value required to be compensated is also smaller, the corresponding error is also smaller, and the result is also more accurate; and respectively compensating the luminous efficiency corresponding to the first scanning moment, the second scanning moment and the third scanning moment according to the maximum value of the luminous efficiency, so that the value of the number of the fluorescent tumors to be detected later is more accurate.

In one possible implementation manner, in the step of aligning the first scanning time, the second scanning time and the third scanning time with the luminous efficiency value corresponding to the preset scanning time according to the preset scanning time corresponding to the maximum value of the luminous efficiency in the luminous curve, the method specifically includes:

and respectively acquiring a first time difference value, a second time difference value and a third time difference value of the first scanning time, the second scanning time and the third scanning time and a preset scanning time. According to the technical scheme provided by the invention, the time interval is obtained by obtaining the difference between the time of 3 times of scanning and the preset scanning time and obtaining the absolute value, wherein the preset scanning time is the time corresponding to the highest value of the luminous efficiency in the luminous curve; obtaining the luminous efficiency to be compensated by obtaining the absolute value of the difference value and the function of the luminous curve, for example: the time corresponding to the highest value of the luminous efficiency, that is, the preset scanning time T is 12:50:00 am on 3 months 1 day, the corresponding t=12×3600+50×60+0, that is, t=46200 seconds, and the corresponding first time difference is |t ₁ -t|=3000 seconds, the second time difference being |t ₂ -t|=1197 seconds, the third time difference being |t ₂ -t|=603 seconds, facilitating the subsequent bringing into the function of the luminescence curve to find the luminescence efficiency compensation value to be compensated.

And carrying out alignment processing on the luminous efficiency values of the first scanning moment, the second scanning moment and the third scanning moment according to the first moment difference value, the second moment difference value and the third moment difference value. According to the technical scheme provided by the invention, the first time difference value, the second time difference value and the third time difference value are brought into a function to obtain the corresponding luminous efficiency compensation value, for example: the first time difference is |T ₁ -t|=3000 seconds into the function of the luminescence curve, corresponding T ₁ The luminous efficiency compensation value at the moment isBeta is a constant and is required to be formulated according to different scenes and equipment, and can be 1, 2, 3 and the like, the constant is not limited, the constant is required to be valued according to actual conditions, and k is required to be calculated ₁ For the first compensation weight and the maximum value of the luminous curve function, the weight needs to be adjusted according to the specific fluorescent reagent, and the subsequent compensation of the tumor fluorescence quantity is facilitated by obtaining the difference value between the luminous efficiency value at the first moment and the luminous efficiency value at the maximum moment.

And step S1104, performing fluorescence compensation on the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number according to the luminous efficiency difference value after the alignment treatment to obtain a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number and a compensated third measured fluorescent tumor number.

According to the technical scheme provided by the invention, after the luminous efficiency alignment corresponding to the moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number is compensated to the maximum value based on the fluorescent luminous curve corresponding to the used fluorescent detection agent, the numerical values of the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number are respectively increased to obtain the corresponding actual fluorescent tumor number, namely the compensated measured fluorescent tumor number. For example: if the luminescence curve of the corresponding fluorescence detection agent is an ideal normal distribution diagram, according to the time difference value |T ₁ The luminous efficiency compensation value corresponding to-t|=3000 isAnd compensating the number of the fluorescent tumors, and correspondingly obtaining the compensated first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number, wherein the number after fluorescent compensation is more accurate, so that the follow-up data is more accurate.

In one possible implementation manner, the method specifically includes the steps of:

wherein ,S₁₁ For the compensated first measurement of the number of fluorescent tumors, S ₂₂ For the compensated second measurement of the number of fluorescent tumors, S ₃₃ For the compensated third measurement of the number of fluorescent tumors, S ₁ First measurement of the fluorescent tumor quantity for the fluorescent scanner measurement S ₂ Second measurement of the fluorescent tumor quantity for the fluorescent scanner measurement S ₃ Third measurement of fluorescent tumor number measured for fluorescent scanner, beta is constant, k ₁ For the first compensation weight, k ₂ For the second compensation weight, k ₃ For the third compensation weight, the first compensation weight,for a first luminous efficacy compensation value, +.>For the second luminous efficacy compensation value, +.>Third luminous efficiency compensation value, T ₁ For the first scanning time, T ₂ For the second scanning time, T ₃ For the third scanning time, |T ₁ -t| is the difference between the first scanning instant and the preset scanning instant, |t ₂ -t| is the difference between the second scanning instant and the preset scanning instant, |t ₃ -T|The difference value between the third scanning time and the preset scanning time is obtained; />For a first measurement of the offset value of the fluorescent tumor number,for a second measurement of the offset value of the fluorescent tumor number,for the compensation value of the third measured fluorescent tumor number, the technical scheme provided by the invention compensates the measured fluorescent tumor number by using the compensation value to finally obtain the compensated measured fluorescent tumor number,/the method comprises the following steps of>To compensate for the first measured fluorescent tumor number,to compensate for the second measurement of the number of fluorescent tumors,the number of fluorescent tumors was measured for the third measurement after compensation. According to the technical scheme provided by the invention, the subsequent predicted change value is more accurate through compensating the number of fluorescent tumors, so that the result is more accurate.

And step S120, generating a first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time.

According to the technical scheme provided by the invention, the processor automatically generates the compensated first tumor change value based on the Kramer rule according to the compensated first measured fluorescent tumor number, the compensated second measured fluorescent tumor number, the first scanning time and the second scanning time, and the processor automatically generates the compensated second tumor change value based on the Kramer rule according to the compensated second measured fluorescent tumor number, the compensated third measured fluorescent tumor number, the second scanning time and the third scanning time, and it can be understood that circulating tumor cells are increased by a certain change value, for example: 1 circulating tumor cell becomes 2 circulating tumor cells within a period of time, 2 circulating tumor cells become 4 circulating tumor cells within a period of time, 4 circulating tumor cells become 8 circulating tumor cells within a period of time, and the growth speed of the tumor cells is not increased at a constant speed but increased by a geometric multiple, so that the judgment is most accurate through the tumor change value, and a doctor can make more accurate trend judgment according to the data.

In one possible implementation manner, the method for generating the first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating the second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time specifically includes:

And generating a compensated first tumor change value by using the compensated first measured fluorescent tumor number, the compensated second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a compensated second tumor change value according to the compensated second measured fluorescent tumor number, the compensated third measured fluorescent tumor number, the second scanning time and the third scanning time. According to the technical scheme provided by the invention, the original directly measured fluorescent tumor quantity is replaced by the compensated fluorescent tumor quantity, so that the result is more accurate, and the subsequent tumor change value is more in line with reality and more accurate.

And generating a compensated first tumor change value by using the compensated first measured fluorescent tumor number, the compensated second measured fluorescent tumor number, the first scanning time and the second scanning time based on the Kramer rule. According to the technical scheme provided by the invention, the change value of the tumor is obtained by utilizing a determinant formed by data of the first measured fluorescent tumor number after compensation, the second measured fluorescent tumor number after compensation and the first scanning moment and the second scanning moment based on the Kramer rule, so that the growth speed of the tumor is predicted.

And generating a compensated second tumor change value by using the compensated second measured fluorescent tumor number, the compensated third measured fluorescent tumor number, the second scanning time and the third scanning time based on the Kramer rule. According to the technical scheme provided by the invention, the processor can generate the second tumor change value and check and feed back the first tumor change value.

wherein ,a₁ For the first tumor change value, a ₂ For the second tumor change value, n ₁ For the first variation weight, n ₂ Is the second variation weight. According to the technical scheme provided by the invention, 2 tumor change values are generated based on the Kramer rule, so that subsequent mutual comparison is facilitated, two tumor change values are obtained through a formula, and are mutually checked to see whether abnormality occurs in detection, and whether the data at the feedback position is abnormal or not can be further judged.

And step S130, comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data.

According to the technical scheme provided by the invention, the automatic scanning device automatically generates the first tumor change value, the second tumor change value and the preset first preset change value, whether abnormality is detected can be known by pairwise comparison, and if so, the larger value in the first tumor change value and the second tumor change value is compared with the first preset change value to obtain whether the circulating tumor trend data of the physical examination object is normal or not. For example: the automatic scanning device generates a first tumor change value of 1/s, the automatic scanning device generates a second tumor change value of 2/s, the first tumor change value and the second tumor change value are firstly compared to see whether the first tumor change value and the second tumor change value are normal, if the first tumor change value and the second tumor change value are in a conforming interval, one of the larger values (the second tumor change value is 2/s) is compared with the first preset change value by 3/s, at the moment, tumor trend data are displayed normally, and it can be understood that the trend is displayed normally if the first tumor change value is smaller than the first preset change value, the trend is displayed abnormally if the first tumor change value is larger than the first preset change value, at the moment, a physical examination object is required to be notified, and more detailed recheck is performed.

In one possible implementation manner, the step S130 specifically includes:

generating a change value difference value by differencing the first tumor change value and the second tumor change value, and taking the larger value of the first tumor change value and the second tumor change value as a target tumor change value; according to the technical scheme provided by the invention, the difference value between the first tumor change value and the second tumor change value is obtained, whether the difference value accords with the preset difference value change value interval or not is checked, and whether the data are normal or not is conveniently judged subsequently. For example: the automatic scanning device generates a first tumor change value of 1/s, the automatic scanning device generates a second tumor change value of 5/s, the second tumor change value can be subtracted from the first tumor change value, the first tumor change value can be subtracted from the second tumor change value, the difference between the corresponding first tumor change value and the corresponding second tumor change value is-4 or +4, and the corresponding target tumor change value is 5/s.

If the variation value difference is not in the preset difference value variation value interval, judging that the detection is abnormal, and entering a reinspection flow; according to the technical scheme provided by the invention, whether abnormality occurs in detection is judged in advance, the difference value of the first tumor change value and the second tumor change value is compared with a preset difference value change value interval, and if the difference value is not, the abnormality is judged to be detected. For example: the difference between the first tumor change value and the second tumor change value is-4 or +4, and the corresponding preset difference change value interval is (-1, 1), at this time, the detection abnormality is judged, and the repeated detection flow is entered to check the equipment or the culture solution.

And if the change value difference is in a preset difference value change value interval, comparing the target tumor change value with a first preset change value to obtain a comparison result, and judging whether tumor trend data are normal or not according to the comparison result. According to the technical scheme provided by the invention, whether detection is normal or not is judged in advance, if the detection is in a preset difference value change value interval, the detection is judged to be normal, the larger value of the first tumor change value and the second tumor change value is correspondingly compared with the first preset change value, whether tumor trend data is normal or not is judged, if the larger value of the first tumor change value and the second tumor change value is larger than the first preset change value, the tumor trend data is judged to be abnormal, and if the larger value of the first tumor change value and the second tumor change value is smaller than the first preset change value, the tumor trend data is judged to be normal. For example: the automatic scanning device generates a first tumor change value of 1/s, the automatic scanning device generates a second tumor change value of 5/s, the difference value between the corresponding first tumor change value and the corresponding second tumor change value is-4 or +4, the preset difference value change value interval is (-10, 10), and the corresponding tumor trend data smaller than the first preset change value is normal when the second tumor change value of 5/s is compared with the first preset change value of 6/s.

In one possible implementation manner, the technical scheme provided by the invention further comprises:

correcting the first change weight and the second change weight according to the physical examination index to obtain a corrected first tumor change value and a corrected second tumor change value; according to the technical scheme provided by the invention, it can be understood that the immune mechanism corresponding to the person with better physical quality is also good, if a large amount of circulating tumor appears in the body, the corresponding immune mechanism can work to kill and phagocytize tumor cells, the person with poor physical quality corresponds to the poor immune mechanism, and although the circulating tumor change value is in a normal range, the circulating tumor is not cleared in time according to the old part with great risk, so that other physical examination data and the weight occupied by the physical examination data are added to correct the change weight, and the generated tumor trend data is more accurate.

The modified first variation weight and the modified second variation weight are obtained by the following formulas,

wherein ,n₃ For the corrected first variation weight, n ₄ For the second modified weight, p is the weight adjustment correction value, N is the normal index number of physical examination, u _i Is the value of the i-th normal index, v _i Is the weight of the i-th normal index, gamma and delta are constants, x _i Is the value of the i-th abnormal index, y _i Weight, x, of class i anomaly index _i *y _i For the degree of influence of the i-th abnormality index, u _i *v _i For the degree of influence of the i-th type of normal index,the numerical value of (2) and n ₃ In direct proportion to each other,the numerical value of (2) and n ₃ In inverse proportion, it is understood that a larger value of the abnormality index corresponds to a larger modified change weight, a larger value of the change corresponding to a tumor, a smaller value of the change corresponding to a smaller change weight corresponding to a tumor. According to the technical scheme provided by the invention, the normal index value and the weight of the normal index value of the corresponding category are taken into the reference, the weight of the abnormal index value of the corresponding category is taken as a measurement of the physical quality of a person, and the tumor trend data is corrected through the physical body, so that the result is more accurate. Wherein, physical examination index can be: blood pressure, urobilinogen, erythrocyte count, hemoglobin, hematocrit, serum amylase, etc.

and classifying the patient culture fluid according to the second preset change value, the target tumor change value and the first preset change value which are actively set by the hospital, and sending the classification result to a corresponding storage area in the server.

As shown in fig. 4, in the server distribution scenario of the automatic scanning apparatus for circulating tumor cells provided by the present invention,the processor firstly judges the difference value of the tumor change values obtained by three times of measurement, and if the difference value is not in the preset difference value change value interval, the processor judges that the detection is abnormal, and the reinspection is possibly needed; if the first tumor change value is within the preset difference change value interval and is smaller than the first preset change value, the processor distributes data to the first storage area, if the first tumor change value is larger than the first preset change value and smaller than the second preset change value, the processor distributes data to the second storage area, if the first tumor change value is larger than the second preset change value, the processor distributes data to the third storage area, and meanwhile, the time corresponding to the first tumor change value is ordered from small to large, and the first person is preferentially notified of the first person. If the target tumor change value is smaller than the first preset change value, marking the detection result of the culture solution as no tumor risk, and distributing the detection result to a first storage area in the server according to the marking, wherein the first storage area is a physical examination center calling area. According to the technical scheme provided by the invention, if the target tumor change value is smaller than a first preset change value, data are stored in a first storage area, the corresponding first storage area is a physical examination center retrieval area, a doctor or nurse can retrieve the data in the first storage area through mobile equipment and inform a physical examination object of taking a physical examination list of the doctor or nurse before the physical examination object, the automatic scanner records a first scanning moment and a machine number of the doctor or nurse, the sorting is carried out according to the moment in the storage process, the sorting is carried out according to the moment, the sorting is carried out from small to large, the notification is carried out according to the time, if two results are obtained at the same moment, the number of an automatic scanning device is attached, and the physical examination result is timely distributed with notification according to the principle that the doctor or nurse comes after the first moment, for example: target tumor change value a ₃ Is 5/s and corresponds to a first preset change value A ₁ 10 pieces/s, in which case A ₁ >a ₃ And the corresponding data codes are stored in the first storage area, and the data is 43200- #897-5-liutao at the moment of corresponding bit scanning, the machine number, the target tumor change value and the name, so that follow-up nurses can conveniently inform according to time sequence.

If the target tumor change value is larger than the first preset change value and smaller than the second preset change value, marking the detection result of the culture solution as moderate tumor risk, and distributing the detection result to a second storage area in the server according to the marking, wherein the second storage area is a common outpatient call area. According to the technical scheme provided by the invention, if the machine-recognized tumor change value is larger than the first preset change value and smaller than the second preset change value, the trend data of the tumor is judged to be abnormal, the possibility of further increase in the future is high, corresponding data are still stored in the second storage area within a controllable range, the corresponding second storage area is a common outpatient retrieval area, a doctor or a nurse can retrieve the data in the second storage area through mobile equipment and inform a physical examination object to carry out recheck, corresponding doctor advice, physical exercise, healthy diet and the like are attached, and a corresponding scheme is formulated.

If the target tumor change value is larger than the second preset change value, marking the detection result of the culture solution as heavy tumor risk, and distributing the detection result to a third storage area in the server according to the mark, wherein the third storage area is an expert outpatient call area. According to the technical scheme provided by the invention, if the first tumor change value is larger than the second preset change value, the abnormal situation of the trend data of the tumor is judged to be serious at the moment, corresponding data are stored in the third storage area, the corresponding third storage area is an expert outpatient service retrieval area, a doctor retrieves the data in the third storage area through mobile equipment and notifies a physical examination object to carry out detailed examination, and a corresponding doctor advice scheme is formulated;

monitoring the behaviors of a doctor to obtain doctor behavior data;

According to the technical scheme provided by the invention, through the subsequent judgment of the tumor trend by the doctor, the adjustment is carried out according to the actual condition of the patient, and the system adjusts at least one of the first preset change value and the second preset change value according to the adjusted data, for example: if the doctor adjusts the severe tumor risk to the moderate tumor risk, the second preset change value is indicated to be inaccurate for automatic adjustment, and it can be understood that if the doctor adjusts the moderate tumor risk to be free of tumor risk, the first preset change value is indicated to be inaccurate, and then the system actively adjusts the first preset change value according to the adjustment of the doctor, so that the first preset change value and the second preset change value are more accurate.

According to the technical scheme provided by the invention, the hospital compares the size of the tumor change value with the second preset change value and the first preset change value set by the hospital, and the physical examination report of the physical examination object is respectively sent to different storage areas and notified by different departments. For example: the tumor change value of the physical examination object is smaller than the first preset change value and is stored in the physical examination center calling area, and then a doctor or a nurse of the physical examination center opens the mobile device to call data from the physical examination center calling area in the server, for example: computers, mobile phones and the like, and notify the corresponding physical examination objects to pick up before.

In one possible implementation manner, in a specific step of adjusting at least one of the first preset change value and the second preset change value if it is determined that the doctor behavior data is any one of a tumor-free risk, a moderate tumor risk and a severe tumor risk, the method specifically includes:

if the doctor behavior data is judged to adjust the non-tumor risk to the moderate tumor risk, the adjustment value and the target tumor change value are actively input to the first preset change value for forward adjustment based on the doctor,

wherein ,A₃ A is a first preset change value after forward adjustment ₄ For doctor to actively input adjustment value, U ₁ Is the first positive adjustment value. The technical proposal provided by the invention obtains a new first preset change value as a demarcation for distinguishing between no tumor risk and moderate tumor risk through the adjustment value actively input by a doctor and the original target tumor change valueThe invention can make the first preset change value more accurate through continuous adjustment of each doctor and big data.

If the doctor behavior data is judged to adjust the moderate tumor risk to be free of tumor risk, the adjustment value and the target tumor change value are actively input to the first preset change value for negative adjustment based on the doctor,

wherein ,A₄ U is a first preset change value after negative adjustment ₂ Is the first negative adjustment value. According to the technical scheme provided by the invention, doctors observe and find that the diagnosis of the system is wrong to actively adjust, the demarcation point (the first preset change value) of the corresponding system for distinguishing the non-tumor risk from the moderate tumor risk is also adjusted continuously, at the moment, the moderate tumor risk is adjusted to be non-tumor risk, and the original first preset change value is adjusted along with the moderate tumor risk, so that the demarcation point is more accurate through continuous adjustment of doctors.

If the doctor behavior data is judged to adjust the moderate tumor risk to the severe tumor risk, the adjustment value and the target tumor change value are actively input to the second preset change value for forward adjustment based on the doctor,

wherein ,A₄ Is a second preset change value after forward adjustment, U ₃ Is the second positive adjustment value. According to the technical scheme provided by the invention, a new second preset change value is obtained through the adjustment value actively input by a doctor and the original target tumor change value and is used as the distinction between the moderate tumor risk and the severe tumor risk.

If the doctor behavior data is judged to adjust the severe tumor risk to the moderate tumor risk, the adjustment value and the target tumor change value are actively input to the second preset change value for forward adjustment based on the doctor,

wherein ,A₆ Is a second preset change value after negative adjustment, U ₄ And is the second negative adjustment value.

According to the technical scheme provided by the invention, continuous learning can be performed through continuous adjustment of doctors, so that the first preset change value and the second preset change value are more accurate, the actual situation is more met, the daily diagnosis requirement of the doctors is met, and the workload of the doctors is reduced.

In the present embodiment, the steps S110 and S130 are not limited by the described order of operation, and the steps S110 and S130 may be performed in other orders or simultaneously.

Fig. 5 is a schematic structural diagram of an automatic scanning device for circulating tumor cells according to an embodiment of the present invention, where the automatic scanning device for circulating tumor cells includes:

As shown in fig. 6, a schematic hardware structure of an electronic device according to an embodiment of the present invention is shown, where the electronic device 20 includes: a processor 21, a memory 22 and a computer program; wherein,

a memory 22 for storing said computer program, which memory may also be a flash memory (flash). Such as application programs, functional modules, etc. implementing the methods described above.

And a processor 21 for executing the computer program stored in the memory to implement the steps executed by the apparatus in the above method. Reference may be made in particular to the description of the embodiments of the method described above.

Alternatively, the memory 22 may be separate or integrated with the processor 21.

When the memory 22 is a device separate from the processor 21, the apparatus may further include:

a bus 23 for connecting the memory 22 and the processor 21.

The present invention also provides a readable storage medium having stored therein a computer program for implementing the methods provided by the various embodiments described above when executed by a processor.

The readable storage medium may be a computer storage medium or a communication medium. Communication media includes any medium that facilitates transfer of a computer program from one place to another. Computer storage media can be any available media that can be accessed by a general purpose or special purpose computer. For example, a readable storage medium is coupled to the processor such that the processor can read information from, and write information to, the readable storage medium. In the alternative, the readable storage medium may be integral to the processor. The processor and the readable storage medium may reside in an application specific integrated circuit (Application Specific Integrated Circuits, ASIC for short). In addition, the ASIC may reside in a user device. The processor and the readable storage medium may reside as discrete components in a communication device. The readable storage medium may be read-only memory (ROM), random-access memory (RAM), CD-ROMs, magnetic tape, floppy disk, optical data storage device, etc.

The present invention also provides a program product comprising execution instructions stored in a readable storage medium. The at least one processor of the device may read the execution instructions from the readable storage medium, the execution instructions being executed by the at least one processor to cause the device to implement the methods provided by the various embodiments described above.

In the above embodiment of the apparatus, it should be understood that the processor may be a central processing unit (english: central Processing Unit, abbreviated as CPU), or may be other general purpose processors, digital signal processors (english: digital Signal Processor, abbreviated as DSP), application specific integrated circuits (english: application Specific Integrated Circuit, abbreviated as ASIC), or the like. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. The steps of a method disclosed in connection with the present invention may be embodied directly in a hardware processor for execution, or in a combination of hardware and software modules in a processor for execution.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.

Claims

1. An automated scanning apparatus for circulating tumor cells, comprising:

the tumor change comparison module is used for comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data;

performing fluorescent scanning on the culture solution with the blood sample at fixed time to generate a first measured fluorescent tumor number, a second measured fluorescent tumor number and a third measured fluorescent tumor number, and recording a first scanning moment, a second scanning moment and a third scanning moment corresponding to the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number, wherein the method specifically comprises the following steps:

performing fluorescence compensation on the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number according to the light-emitting efficiency difference value after the alignment treatment to obtain a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number and a compensated third measured fluorescent tumor number;

according to a preset scanning time corresponding to the maximum value of the luminous efficiency in the luminous curve, aligning the first scanning time, the second scanning time and the third scanning time with luminous efficiency values corresponding to the preset scanning time, wherein the method specifically comprises the following steps:

carrying out alignment processing on luminous efficiency values of the first scanning moment, the second scanning moment and the third scanning moment according to the first moment difference value, the second moment difference value and the third moment difference value;

performing fluorescence compensation on the first measured fluorescent tumor number, the second measured fluorescent tumor number and the third measured fluorescent tumor number according to the light-emitting efficiency difference value after the alignment treatment to obtain a compensated first measured fluorescent tumor number, a compensated second measured fluorescent tumor number and a compensated third measured fluorescent tumor number, wherein the method specifically comprises the following steps:

wherein ,to compensate for the first measured fluorescent tumor number, and (2)>To compensate for the second measurement of the number of fluorescent tumors,to compensate for the third measurement of the number of fluorescent tumors, and (2)>For a first measurement of the fluorescent tumor number measured by the fluorescence scanner,second measurement of the number of fluorescent tumors measured for the fluorescence scanner,/->Third measurement of the fluorescent tumor number measured for the fluorescent scanner,/->Is constant (I)>For the first compensation weight, +.>For the second compensation weight->For the third compensation weight->For the first scanning instant +.>For the second scanning moment, +.>For the third scanning instant +.>For the difference between the first scanning instant and the preset scanning instant,/or->For the difference between the second scanning instant and the preset scanning instant,/or->The difference value between the third scanning time and the preset scanning time is obtained.

2. The apparatus of claim 1, wherein the apparatus comprises a plurality of scanning devices,

generating a first tumor change value by using the first measured fluorescent tumor number, the second measured fluorescent tumor number, the first scanning time and the second scanning time, and generating a second tumor change value according to the second measured fluorescent tumor number, the third measured fluorescent tumor number, the second scanning time and the third scanning time, wherein the method specifically comprises the following steps:

wherein ,for the first tumor change value, < >>For the second tumor change value, < >>For the first variation weight ∈>Is the second variation weight.

3. The automated circulating tumor cell scanning apparatus of claim 2, further comprising:

correcting the first change weight and the second change weight according to the physical examination index to obtain corrected first change weight and corrected second change weight;

The corrected first variation weight and the corrected second variation weight are obtained by the following formulas,

wherein ,adjusting the weight for the corrected first change,/->Adjusting the weight for the modified second variation,/->For the weight adjustment correction value, +>Is the normal index number of physical examination->Is->Value of normal-like index,/->Is->Weight of class normal index, ++> and />Is constant (I)>Is the abnormal index number of physical examination->Is->Numerical value of abnormality-like index->Is->Weight of class anomaly index, ++>Is->Degree of influence of abnormality-like index, ++>Is->The degree of influence of the normal-like index.

4. The apparatus for automatically scanning circulating tumor cells according to claim 3,

comparing the first tumor change value, the second tumor change value and the first preset change value to generate tumor trend data, wherein the tumor trend data specifically comprises:

5. The automated circulating tumor cell scanning apparatus of claim 4, further comprising:

classifying the patient culture fluid according to a second preset change value, the target tumor change value and the first preset change value which are actively set by the hospital, and sending the classification result to a corresponding storage area in the server;

Monitoring the behaviors of a doctor to obtain doctor behavior data;