CN115717098A - Frozen soil microorganism culture equipment - Google Patents

Frozen soil microorganism culture equipment Download PDF

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Publication number
CN115717098A
CN115717098A CN202211434458.9A CN202211434458A CN115717098A CN 115717098 A CN115717098 A CN 115717098A CN 202211434458 A CN202211434458 A CN 202211434458A CN 115717098 A CN115717098 A CN 115717098A
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CN
China
Prior art keywords
fixedly connected
microorganism culture
plate
frozen soil
soil microorganism
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Application number
CN202211434458.9A
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Chinese (zh)
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CN115717098B (en
Inventor
马大龙
马勇
范广丽
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Harbin Normal University
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Harbin Normal University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The invention relates to microorganism culture, in particular to frozen soil microorganism culture equipment, which comprises a support ring, wherein a connecting plate is fixedly connected onto the support ring, a support plate is fixedly connected onto the support ring, a closed bottom ring is fixedly connected onto the support ring, a plurality of inserting bulges are arranged on the closed bottom ring, each inserting bulge is provided with an inserting groove, a support frame is fixedly connected onto the connecting plate, and a support bottom plate is fixedly connected onto the support frame; rotate on the support frame and be connected with the drive wheel, rotate on the support frame and be connected with from the driving wheel, the drive wheel is connected through the driving band transmission between the follow driving wheel, a plurality of spacing archs of fixedly connected with on the driving band, fixedly connected with drive wheel carries out pivoted power unit I on the support frame, can be orderly accomodate the incubator with the microorganism culture dish in to can be according to the user demand of difference, can take out the microorganism culture dish of an arbitrary position.

Description

Frozen soil microorganism culture equipment
Technical Field
The invention relates to microorganism culture, in particular to frozen soil microorganism culture equipment.
Background
Microorganism culture, which means that certain microorganisms grow and propagate rapidly by means of artificially prepared culture media and artificially created culture conditions, and is called microorganism culture; when cultivateing the microorganism among the prior art, often need place the microorganism in the culture dish, and place the culture dish in the incubator, and because the incubator is the square often when placing, place a plurality of culture dishes in proper order the in-process in the incubator, because a plurality of culture dishes from supreme placing in proper order down, consequently the culture dish of upside can shelter from the culture dish of inside or downside, when any culture dish is taken out to needs, often inconvenient taking out, consequently can't be according to the user demand of difference, take out any culture dish fast, do not influence other culture dishes simultaneously.
Disclosure of Invention
The invention aims to provide frozen soil microorganism culture equipment which can orderly store microorganism culture dishes into an incubator and can take out the microorganism culture dishes at any position according to different use requirements.
The purpose of the invention is realized by the following technical scheme:
a frozen soil microorganism culture device comprises a support ring, wherein a connecting plate is fixedly connected onto the support ring, a support plate is fixedly connected onto the support ring, a closed bottom ring is fixedly connected onto the support ring, a plurality of inserting bulges are arranged on the closed bottom ring, each inserting bulge is provided with an inserting groove, a support frame is fixedly connected onto the connecting plate, and a support bottom plate is fixedly connected onto the support frame;
the supporting frame is rotatably connected with a driving wheel, the supporting frame is rotatably connected with two driven wheels, the driving wheel and the two driven wheels are in transmission connection through two driving belts, the driving belts are fixedly connected with a plurality of limiting bulges, the supporting frame is fixedly connected with a power mechanism I for driving the driving wheel to rotate, and the power mechanism I is preferably a servo motor;
the connecting plate is rotatably connected with a swing shaft, the swing shaft is fixedly connected with a swing plate, the connecting plate is fixedly connected with a power mechanism II for driving the swing shaft to swing, the power mechanism II is preferably a servo motor, the swing plate is positioned between two driven wheels, and the supporting bottom plate is positioned between two driving belts;
the closed bottom ring is rotatably connected with two rotating side plates, the closed bottom ring is fixedly connected with a power mechanism III for driving the rotating side plates to rotate, and the power mechanism III is preferably a servo motor;
the placing bottom plates are connected between the two rotating side plates in a transmission mode, a power mechanism IV for driving the placing bottom plates to rotate is fixedly connected to the rotating side plates, the power mechanism IV is preferably a servo motor, the placing bottom plates are fixedly connected with two telescoping mechanisms I, a clamping plate is fixedly connected to the telescoping end of each telescoping mechanism I, and a sensor is fixedly connected to the placing bottom plates;
the supporting plate is fixedly connected with an arc cavity, the outer sides of the two rotating side plates are rotatably connected to the arc cavity, the arc cavity is fixedly connected with a plurality of extending pipelines, each extending pipeline is provided with a one-way cone, the one-way cone is preferably made of elastic materials, and the arc cavity is fixedly connected with a plurality of ventilating pipelines;
the arc cavity is fixedly connected with a plurality of telescopic mechanisms II, the telescopic end of each telescopic mechanism II is fixedly connected with a sealing plate, the sealing plate can be inserted into the slot, the sealing plate can divide the arc cavity to enable the arc cavity to form a plurality of independent cavities, a plurality of extension pipelines are respectively communicated with the independent cavities, and a plurality of ventilation pipelines are respectively communicated with the independent cavities;
a horizontal sensor is arranged on the placing bottom plate and connected with a power mechanism IV, and the sensor is connected with a telescopic mechanism I.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic structural view of a frozen soil microorganism culture apparatus according to the present invention;
FIG. 2 is a schematic view of the support ring structure of the present invention;
FIG. 3 is a schematic view of a closed bottom ring structure of the present invention;
FIG. 4 is a schematic view of the support base structure of the present invention;
FIG. 5 is a schematic view of the drive wheel configuration of the present invention;
FIG. 6 is a schematic view of the swing plate construction of the present invention;
FIG. 7 is a schematic view of the pivoting side panel of the present invention;
FIG. 8 is a schematic view of the placement floor configuration of the present invention;
FIG. 9 is a schematic view of a circular arc chamber structure of the present invention;
FIG. 10 is a schematic view of the closure panel construction of the present invention;
FIG. 11 is a schematic view of a one-way cone structure of the present invention;
FIG. 12 is a schematic view of the one-way cone structure of the present invention.
In the figure:
a support ring 11; a connecting plate 12; a support plate 13; a closed bottom ring 14; the insertion projection 15; a slot 16; a support frame 17; a support base plate 18;
a drive wheel 21; a driven wheel 22; a drive belt 23; a limiting bulge 24;
a swing shaft 31; a swing plate 32;
rotating the side plate 40;
placing the bottom plate 51; a telescoping mechanism I52; a clamping plate 53; a sensor 54;
a circular arc cavity 61; into the duct 62; a one-way taper 63; an air duct 64;
a telescoping mechanism II 71; a closing plate 72.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in FIG. 1, the structure and function of a frozen soil microorganism culture apparatus will be described in detail;
a frozen soil microorganism culture device comprises a support ring 11, wherein a connecting plate 12 is fixedly connected to the support ring 11, a support plate 13 is fixedly connected to the support ring 11, a closed bottom ring 14 is fixedly connected to the support ring 11, a plurality of inserting protrusions 15 are arranged on the closed bottom ring 14, each inserting protrusion 15 is provided with an inserting groove 16, a support frame 17 is fixedly connected to the connecting plate 12, and a support bottom plate 18 is fixedly connected to the support frame 17;
the supporting frame 17 is rotatably connected with a driving wheel 21, the supporting frame 17 is rotatably connected with two driven wheels 22, the driving wheel 21 is in transmission connection with the two driven wheels 22 through two driving belts 23, the driving belts 23 are fixedly connected with a plurality of limiting protrusions 24, the supporting frame 17 is fixedly connected with a power mechanism I for driving the driving wheel 21 to rotate, and the power mechanism I is preferably a servo motor;
the connecting plate 12 is rotatably connected with a swinging shaft 31, the swinging shaft 31 is fixedly connected with a swinging plate 32, the connecting plate 12 is fixedly connected with a power mechanism II for driving the swinging shaft 31 to swing, the power mechanism II is preferably a servo motor, the swinging plate 32 is positioned between two driven wheels 22, and the supporting bottom plate 18 is positioned between two driving belts 23;
the closed bottom ring 14 is rotatably connected with two rotating side plates 40, the closed bottom ring 14 is fixedly connected with a power mechanism III for driving the rotating side plates 40 to rotate, and the power mechanism III is preferably a servo motor;
a plurality of placing bottom plates 51 are connected between the two rotating side plates 40 in a transmission manner, a power mechanism IV for driving the placing bottom plates 51 to rotate is fixedly connected to the rotating side plates 40, the power mechanism IV is preferably a servo motor, the placing bottom plates 51 are fixedly connected with two telescopic mechanisms I52, the telescopic end of each telescopic mechanism I52 is fixedly connected with a clamping plate 53, and the placing bottom plates 51 are fixedly connected with sensors 54;
the supporting plate 13 is fixedly connected with an arc cavity 61, the outer sides of the two rotating side plates 40 are rotatably connected to the arc cavity 61, the arc cavity 61 is fixedly connected with a plurality of extending pipelines 62, each extending pipeline 62 is provided with a one-way cone 63, the one-way cones 63 are preferably made of elastic materials, and the arc cavity 61 is fixedly connected with a plurality of ventilating pipelines 64;
the arc cavity 61 is fixedly connected with a plurality of telescopic mechanisms II 71, the telescopic end of each telescopic mechanism II 71 is fixedly connected with a sealing plate 72, the sealing plate 72 can be inserted into the slot 16, the sealing plate 72 can divide the arc cavity 61, so that the arc cavity 61 forms a plurality of independent cavities, the plurality of extension pipelines 62 are respectively communicated with the plurality of independent cavities, and the plurality of vent pipelines 64 are respectively communicated with the plurality of independent cavities;
the sensor 54 is connected with the telescopic mechanism I52;
when the culture dish storage device is used, a culture dish is disinfected in advance, a culture solution and microorganisms needing to be cultured are placed in the culture dish, the culture dish is placed on the placement bottom plate 51, the telescoping mechanism I52 is started, the telescoping mechanism I52 can be a hydraulic cylinder or an electric push rod, the telescoping end of the telescoping mechanism I52 drives the clamping plate 53 to move, the two clamping plates 53 move close to each other, the culture dish is clamped by the two clamping plates 53, the power mechanism III is started, the output shaft of the power mechanism III starts to rotate, the output shaft of the power mechanism III drives the placement bottom plates 51 to rotate, the positions of the placement bottom plates 51 are changed, the placement bottom plates 51 are stored in the arc cavity 61, the placement bottom plates 51 sequentially pass through the open ends of the arc cavity 61, the culture dish is placed on the placement bottom plate 51 continuously, the power mechanism III is started intermittently, the output shaft of the power mechanism III drives the placement bottom plates 51 to be stored in the arc cavity 61, and the storage of the culture dishes is completed;
when any culture dish needs to be taken out, only the power mechanism III needs to be started, an output shaft of the power mechanism III starts to rotate, the output shaft of the power mechanism III drives the rotating side plate 40 to rotate, the rotating side plate 40 drives the placing bottom plates 51 to rotate, so that the placing bottom plates 51 move to openings of the arc cavities 61, as shown in fig. 9, the telescoping mechanism I52 is started, a telescoping end of the telescoping mechanism I52 drives the clamping plates 53 to move, the two clamping plates 53 do not clamp the culture dish any more, and the culture dish is taken out from the placing bottom plate 51;
further, a power mechanism iv for driving the placing bottom plate 51 to rotate is fixedly connected to the rotating side plate 40, in order to ensure that the placing bottom plate 51 is always in a horizontal state, the power mechanism iv is started, an output shaft of the power mechanism iv starts to rotate, the output shaft of the power mechanism iv drives the placing bottom plate 51 to rotate, and it is ensured that the placing bottom plates 51 are always in a horizontal state in the process that the rotating side plate 40 rotates the placing bottom plates 51;
further, a horizontal sensor is arranged on the placing bottom plate 51 and connected with the power mechanism iv, the horizontal sensor can be a commonly used sensor for balance in the prior art, such as a gyroscope and the like, and in order to facilitate the rotation of an output shaft of the power mechanism iv to control the horizontal state of the placing bottom plate 51, the horizontal sensor is connected with the power mechanism iv through a commonly used technical means in the field, so that the output shaft of the power mechanism iv is conveniently controlled, and the placing bottom plates 51 are ensured to be in a horizontal state in the movement process;
further, the telescopic mechanism II 71 is started, the telescopic end of the telescopic mechanism II 71 drives the sealing plate 72 to move, so that the sealing plate 72 slides on the arc cavity 61, the sealing plate 72 slides downwards, the sealing plate 72 is inserted into the slot 16, the sealing plate 72 divides the space between the arc cavity 61 and the two rotating side plates 40 to form a plurality of independent cavities, a plurality of heating devices are arranged in the arc cavity 61 and are respectively arranged in the independent cavities, different microorganisms can be placed in each culture dish, and then the heating devices can heat different independent cavities to different temperatures, so that different use requirements are met;
further, as shown in fig. 9, the plurality of vent pipes 64 are respectively communicated with the plurality of independent cavities, some microorganisms need to be cultured in an aerobic environment, and some microorganisms need to be cultured in an anaerobic environment, so that different gas pipes are connected to the vent pipes 64 in advance according to different use requirements, and the vent pipes 64 are provided with valves which are opened to introduce different gases into the independent cavities, thereby meeting the culture requirements of different microorganisms;
furthermore, the plurality of extending pipes 62 are respectively communicated with the plurality of independent cavities, a cover plate is arranged on the extending pipes 62, which is not shown in the figure, and the cover plate is detachably and fixedly connected to the extending pipes 62, so that when part of the microorganisms in the culture dish need to be taken out for observation or certain culture substances need to be added to the culture dish, the cover plate is taken down from the extending pipes 62, and the microorganisms on the culture dish are taken out for observation or the culture substances are added to the culture dish through the extending pipes 62;
further, as shown in fig. 11 and 12, in order to ensure that the culture environment in the independent cavity is not damaged when adding substances into the culture dish and taking out a microorganism sample, a one-way cone 63 is further provided, the one-way cone 63 is preferably made of rubber, the one-way cone 63 is conical, and the extraction of microorganisms in the culture dish or the addition of substances into the culture dish can be completed by passing a forceps or a dropper through the one-way cone 63, because the one-way cone 63 is made of an elastic material, the one-way cone 63 can coat the tool, thereby reducing the damage to the culture environment in the independent cavity;
further, as shown in fig. 5, for convenience of placing a plurality of culture dishes on the plurality of placing bottom plates 51 in sequence, a driving belt 23 capable of driving the plurality of culture dishes to move is further provided;
when the culture dish placing device is used, a plurality of culture dishes are sequentially placed on the two driving belts 23, the culture dishes are located between the two limiting protrusions 24 on the two sides, the power mechanism I is started, the output shaft of the power mechanism I starts to rotate, the output shaft of the power mechanism I drives the driving wheel 21 to rotate, the driving wheel 21 drives the two driving belts 23 to move, the driving belts 23 drive the culture dishes to move forwards, the culture dishes move to the upper side of the swinging plate 32, the power mechanism II is started, the output shaft of the power mechanism II starts to rotate, the output shaft of the power mechanism II drives the swinging shaft 31 to rotate, the swinging shaft 31 drives the swinging plate 32 to rotate, the swinging plate 32 tilts to lift the culture dishes, the culture dishes are separated from the limiting protrusions 24, the swinging plate 32 tilts the culture dishes to move along the tilting direction of the swinging plate 32, the culture dishes slide to the upper side of the placing bottom plate 51, and the plurality of culture dishes are sequentially placed between the two driving belts 23, and placing of the plurality of culture dishes is completed;
further, a sensor 54 is further arranged, the sensor 54 can be a contact sensor or a squeezing sensor, the sensor 54 is connected with the telescoping mechanism I52 through an electric control means commonly used in the field, when the culture dish is placed on the sensor 54, the sensor 54 is squeezed to control the telescoping end of the telescoping mechanism I52 to move, and the telescoping end of the telescoping mechanism I52 clamps the culture dish;
further, when a plurality of culture dishes in the arc cavity 61 need to be taken out in sequence, the power mechanism III is started, the output shaft of the power mechanism III drives the rotating side plate 40 to rotate, the rotating side plate 40 drives the placing bottom plates 51 to rotate, the placing bottom plates 51 move to the opening of the arc cavity 61 in sequence, the telescoping mechanism I52 is started, the telescoping end of the telescoping mechanism I52 is retracted, clamping of the culture dishes is released, the power mechanism IV is started, the output shaft of the power mechanism IV drives the placing bottom plate 51 to rotate, the placing bottom plate 51 inclines, the height of the placing bottom plate 51 is higher than that of the swinging plate 32, the culture dishes fall on the swinging plate 32 along the inclination of the placing bottom plate 51, the power mechanism I is started, the output shaft of the power mechanism I drives the driving belt 23 to move, the carrying-out of the culture dishes is completed, then the culture dishes move in sequence, and the taking-out of the culture dishes is completed.

Claims (10)

1. A frozen soil microorganism culture apparatus comprising a closed bottom ring (14), characterized in that: the closed bottom ring (14) is connected with two rotating side plates (40) in a rotating mode, the two rotating side plates (40) are connected with arc cavities (61) in a rotating mode, and a plurality of placing bottom plates (51) are connected between the two rotating side plates (40) in a transmission mode.
2. The frozen soil microorganism culture apparatus according to claim 1, wherein: closed end ring (14) fixed connection is on support ring (11), fixedly connected with backup pad (13) on support ring (11), fixedly connected with closed end ring (14) on support ring (11), fixedly connected with support frame (17) on connecting plate (12), fixedly connected with supporting baseplate (18) on support frame (17).
3. The frozen soil microorganism culture apparatus according to claim 2, wherein: the closed bottom ring (14) is provided with a plurality of insertion bulges (15), and each insertion bulge (15) is provided with a slot (16).
4. The frozen soil microorganism culture apparatus according to claim 3, wherein: support frame (17) are gone up to rotate and are connected with drive wheel (21), and support frame (17) are gone up to rotate and are connected with two from driving wheel (22), and drive wheel (21) and two are connected through two driving band (23) transmissions between driving wheel (22), and a plurality of spacing archs (24) of fixedly connected with on driving band (23).
5. The frozen soil microorganism culture apparatus according to claim 4, wherein: the connecting plate (12) is rotatably connected with a swinging shaft (31), and the swinging shaft (31) is fixedly connected with a swinging plate (32).
6. The frozen soil microorganism culture apparatus according to claim 5, wherein: the swing plate (32) is located between two driven wheels (22) and the support base (18) is located between two drive belts (23).
7. The frozen soil microorganism culture apparatus according to claim 5, wherein: the placing bottom plate (51) is fixedly connected with two telescopic mechanisms I (52), and the telescopic end of each telescopic mechanism I (52) is fixedly connected with a clamping plate (53).
8. The frozen soil microorganism culture apparatus according to claim 7, wherein: the placing bottom plate (51) is fixedly connected with a sensor (54), the sensor (54) is connected with a telescopic mechanism I (52), and a horizontal sensor is arranged on the placing bottom plate (51).
9. The frozen soil microorganism culture apparatus according to claim 7, wherein: fixedly connected with circular arc cavity (61) on backup pad (13), the outside of two rotation curb plates (40) is rotated and is connected on circular arc cavity (61), and fixedly connected with is a plurality of pipeline (62) of stretching into on circular arc cavity (61), and every stretches into all to be provided with one-way cone (63) on pipeline (62), and a plurality of air duct (64) of fixedly connected with are gone up to circular arc cavity (61).
10. The frozen soil microorganism culture apparatus according to claim 9, wherein: a plurality of telescopic machanisms II (71) of fixedly connected with on circular arc cavity (61), equal fixedly connected with closing plate (72) are served in the flexible of every telescopic machanism II (71), in closing plate (72) can insert slot (16), closing plate (72) can be cut apart circular arc cavity (61) for circular arc cavity (61) form a plurality of independent cavities, a plurality of pipelines (62) of stretching into communicate with a plurality of independent cavities respectively, a plurality of vent-to-air ducts (64) communicate with a plurality of independent cavities respectively.
CN202211434458.9A 2022-11-16 2022-11-16 Frozen soil microorganism culture equipment Active CN115717098B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117165422A (en) * 2023-11-03 2023-12-05 哈尔滨师范大学 Microorganism sample collection device

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CN112877183A (en) * 2021-01-29 2021-06-01 武汉兰卫医学检验实验室有限公司 Medical science inspection microbiological culture apparatus
CN114736800A (en) * 2022-04-18 2022-07-12 哈尔滨医科大学 Embryo incubator with built-in microscopic imaging system
CN114874905A (en) * 2022-06-14 2022-08-09 赵倩 Stem cell proliferation culture method and application thereof

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US20130229168A1 (en) * 2010-11-17 2013-09-05 Panasonic Corporation Microorganism number measurement device
JP2017042145A (en) * 2015-08-28 2017-03-02 株式会社エヌテック Microorganism inspection apparatus and microorganism inspection method
CN110195012A (en) * 2019-05-30 2019-09-03 四川若斌生物科技有限责任公司 A kind of microorganism cultivates detection device and microculture detection method automatically
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CN117165422B (en) * 2023-11-03 2024-01-26 哈尔滨师范大学 Microorganism sample collection device

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