CN115716868B - Transcription factor MrPigB mutant and application thereof - Google Patents
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- 108091023040 Transcription factor Proteins 0.000 title abstract description 11
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Abstract
The invention belongs to the field of bioengineering, and particularly relates to a transcription factor MrPigB mutant and application thereof. The specific technical scheme is as follows: an MrPigB mutant, which is mutated on the basis of the original MrPigB, the mutation site comprising: the glutamic acid at the 25 th site in the original sequence is mutated to glutamine, the arginine at the 32 nd site is mutated to glutamic acid, the lysine at the 41 st site is mutated to asparagine, and the serine at the 46 th site is mutated to threonine; the amino acid sequence of the original MrPigB is shown as SEQ ID NO. 1. The invention mutates the key site amino acid of transcription factor MrPigB to obtain MrPigB mutant. The yield of monascus pigment is improved by first mutating MrPigB, and the yield and proportion of monascus pigment are effectively improved. The monascus strain carrying the mutant MrPigB improves the proportion of monascus yellow pigment in the total monascus pigment from 27% to 72%, and simultaneously improves the total monascus pigment yield by 15% compared with the original strain.
Description
Technical Field
The invention belongs to the field of bioengineering, and particularly relates to a transcription factor MrPigB mutant and application thereof.
Background
Monascus pigment is a widely used food colorant prepared by fermentation of Monascus spp (also known as Monascus purpureus), and mainly comprises Monascus haematochrome, monascus flavochrome and Monascus orange pigment. The prior researches find that: the monascus yellow pigment is bright in color and has various biological activities, such as inhibiting the growth of cancer cells, diminishing inflammation, resisting bacteria, reducing blood fat and the like. Thus, monascus yellow pigment is a new research hotspot. However, under natural conditions, the content of monascus yellow pigment is relatively low or even the lowest among three monascus pigments produced by monascus. Therefore, how to improve the ability of monascus to produce monascus yellow pigment becomes an important subject in current industrial production.
Biosynthesis of monascus pigment is a very complex multienzyme tandem catalytic process. In the monascus genome, related genes responsible for monascus pigment synthesis are clustered together and are called a monascus pigment biosynthesis gene cluster. The gene cluster comprises 14 enzyme or transporter genes and 2 transcription factors: mrPigB and MrPigI. Transcription factors are a class of proteins that specifically recognize and bind to a particular promoter fragment, thereby activating or inhibiting gene expression downstream of the promoter. MrPigB is a transcription factor responsible for activating monascus pigment biosynthesis gene cluster, and its function plays a vital role in monascus pigment synthesis. However, there is currently no report on modification of the MrPigB gene to regulate monascus pigment synthesis.
Disclosure of Invention
The invention aims to provide a transcription factor MrPigB mutant and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme: an MrPigB mutant, which is mutated on the basis of the original MrPigB, the mutation site comprising: the glutamic acid at the 25 th site in the original sequence is mutated to glutamine, the arginine at the 32 nd site is mutated to glutamic acid, the lysine at the 41 st site is mutated to asparagine, and the serine at the 46 th site is mutated to threonine; the amino acid sequence of the original MrPigB is shown as SEQ ID NO. 1.
Correspondingly, the amino acid sequence of the MrPigB mutant is shown as SEQ ID NO. 2.
Correspondingly, the MrPigB mutant is applied to monascus recombination.
Correspondingly, recombinant monascus prepared by using the MrPigB mutant.
Correspondingly, the MrPigB mutant is applied to improving the yield of monascus pigment produced by monascus.
Correspondingly, the MrPigB mutant is applied to improving the capacity of monascus to produce monascus yellow pigment.
The invention has the following beneficial effects: the invention mutates the key site amino acid of transcription factor MrPigB to obtain MrPigB mutant. The yield of monascus pigment is improved by first mutating MrPigB, and the yield and proportion of monascus pigment are effectively improved. The monascus strain carrying the mutant MrPigB improves the proportion of monascus yellow pigment in the total monascus pigment from 27% to 72%, and simultaneously improves the total monascus pigment yield by 15% compared with the original strain.
Detailed Description
The invention provides a mutant of transcription factor MrPigB, the amino acid sequence of the original MrPigB is shown as SEQ ID NO.1, the amino acid sequence of the mutated MrPigB is shown as SEQ ID NO.2, and the mutation mode is as follows: glutamate at position 25 in the original sequence is mutated to glutamine, arginine at position 32 is mutated to glutamate, lysine at position 41 is mutated to asparagine, and serine at position 46 is mutated to threonine.
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated. The obtained data are all average values obtained after at least 3 repetitions, and each repetition is obtained as effective data.
Examples
1. Monascus ATCC 96218 was inoculated on PDA slants and incubated at 28 ℃ for 7 days. It should be noted that: the strains used in the examples are illustrative and not limiting, and the scheme provided by the invention has universality to monascus. After the cultivation is completed, the mycelium is scraped, and liquid nitrogen is added for rapid grinding into powder. Adding TRIZOL solution, shaking, standing for 5min, adding chloroform solution, shaking, and standing at room temperature for 5min. The supernatant was kept and an equal volume of isopropanol was added and mixed well and allowed to stand to precipitate the RNA molecules. The supernatant was then centrifuged and removed, and the precipitate was washed with 75% ethanol. After removing the supernatant again, double distilled water was added to obtain a total RNA solution. By BeyoRT TM II cDNA Synthesis reagentThe cDNA is prepared by using RNA as a template. Meanwhile, the genome of monascus ATCC 96218 is obtained by extraction with phenol-chloroform.
2. And (3) cloning by using cloning primers (SEQ ID NO.3 and SEQ ID NO. 4) and using the cDNA prepared in the step (1) as a template to obtain the coding sequence of the MrPigB gene through a PCR technology. And designing a pair of homology arms, and replacing the original MrPigB gene with a DNA fragment of the MrPigB mutant by using a homologous recombination principle, wherein the DNA fragment of the homology arms is cloned by using a monascus genome as a template through a PCR technology.
And connecting the upstream and downstream homology arm fragments to the upstream and downstream of the MrPigB mutant gene fragment by an overlap extension PCR method to obtain a fusion fragment. The universal plasmid pCB301 was then cleaved by the restriction enzyme SphI to give a linear plasmid. And assembling the linear plasmid and the fusion fragment by using a multi-fragment one-step cloning kit (Hieff CloneTM Multi One Step Pcr Cloning Kit) to obtain a recombinant plasmid, and after converting the escherichia coli strain TOP10, picking positive clones to extract the recombinant plasmid for later use.
3. The whole recombinant plasmid is replicated by PCR technology through primers (SEQ ID NO.5 and SEQ ID NO. 6) by taking the recombinant plasmid as a template, and glutamic acid at the 25 th site is mutated into glutamine. The obtained PCR product is used for transforming the escherichia coli strain TOP10, a single colony of positive clone is selected for culture, then plasmid is extracted, and sequencing is carried out to verify that mutation is correct. The plasmid is used as a template, and the original MrPigB gene is mutated by adopting primers (SEQ ID NO.7 and SEQ ID NO.8, SEQ ID NO.9 and SEQ ID NO.10, SEQ ID NO.11 and SEQ ID NO. 12) in sequence according to the method, correspondingly, arginine at the 32 th site is mutated to glutamic acid, lysine at the 41 st site is mutated to asparagine, and serine at the 46 th site is mutated to threonine. The mutation site was verified by sequencing the MrPigB gene on the plasmid.
4. Mixing the prepared recombinant plasmid with agrobacterium competent cells, immersing in liquid nitrogen for 5min for precooling, adding into fresh LB culture medium after water bath at 37 ℃ for 5min, and re-culturing at 28 ℃ for 5h. The re-cultured bacterial liquid was spread on LB medium plates containing kanamycin (50. Mu.g/mL). After 2 days of incubation at 28℃single colonies were picked for incubation, and plasmids were extracted and verified by PCR to determine the correct recombinant Agrobacterium.
5. Culturing recombinant Agrobacterium to broth OD using LB Medium 600 0.8 to 1.2, and fresh induction culture medium is added to dilute the OD of the agro-pole fermentation liquid 600 Culturing at 28 deg.c and 150r/min for 6 hr to 0.4-0.6 to obtain agrobacterium liquid. The induction medium consists of: NH (NH) 4 NO 3 0.5g/L，NaCl0.3g/L，CaCl 2 ·2H 2 O 0.01g/L，MgSO 4 ·7H 2 O 0.6g/L，ZnSO 4 ·7H 2 O 0.5mg/L，Na 2 -EDTA·2H 2 O 1.3mg/L。
Inoculating Monascus purpureus ATCC 96218 to CYA medium slant, culturing at 28deg.C for 5d, washing with sterile water to obtain spore liquid (5×10) 5 and/mL). Adding the obtained agrobacterium liquid into monascus spore liquid until the spore liquid concentration is 10 5 And each mL, a mixture was obtained. The mixture is spread on an induction culture medium plate and cultured for 2-3 d at 28 ℃. Picking single colony to fresh PDA culture medium (containing 30mg/L hygromycin B), culturing at 28 deg.C for 5 days, collecting mycelium, extracting genome by phenol-chloroform method, cloning by PCR method using primer (sequence is shown as SEQ ID NO. 3) to obtain mutant MrPigBm gene fragment, sequencing to verify DNA sequence correctness, and obtaining recombinant monascus strain K1.
6. The original monascus strain ATCC 96218 and the recombinant monascus strain K1 were inoculated into PDB medium, respectively, and cultured at 28℃for 7 days at 150 r/min. Mycelium is collected and added with 80% ethanol solution, and extracted for 1h at 50 ℃. The mycelia were removed by filtration, and the supernatant was collected, diluted with ethanol and analyzed for monascus pigment fraction by HPLC. The results show that: the total yield of monascus pigment of the original monascus strain ATCC 96218 is 6840U/L, wherein the mass ratio of monascus pigment to the total monascus pigment is 27%. Recombinant monascus strain K1 carrying MrPigB mutant, total yield of monascus pigment is improved by 15% and 7870U/L is achieved; through quantitative analysis, the mass ratio of the monascus yellow pigment to the total monascus pigment is improved to 72 percent.
The same comparative experiments were performed by transferring mutant MrPigB into other commercially available monascus strains (the same experiment was performed using 10 different sources of monascus respectively), and the results all showed that: the total yield of monascus pigment is obviously improved by replacing the recombinant monascus strain obtained after the original MrPigB is replaced by the MrPigB mutant, the minimum improvement is 820U/L, and the maximum improvement is 1530U/L; meanwhile, the mass ratio of the monascus yellow pigment in the total monascus pigment is improved to more than 60 percent.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications, variations, alterations, substitutions made by those skilled in the art to the technical solution of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the design of the present invention.
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Claims (5)
1. An MrPigB mutant characterized by: the amino acid sequence of the MrPigB mutant is shown as SEQ ID NO. 2.
2. Use of the MrPigB mutant according to claim 1 in recombination of monascus.
3. Recombinant monascus prepared using the MrPigB mutant of claim 1.
4. Use of the MrPigB mutant according to claim 1 for improving the monascus pigment production capacity.
5. The use of the MrPigB mutant according to claim 1 for improving the ability of monascus to produce monascus yellow pigment.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008023469A1 (en) * | 2006-08-25 | 2008-02-28 | Osaka University | Method of identifying transcriptional factor inducing the production of monascus-origin physiologically active substance and utilization thereof |
| CN113308443A (en) * | 2021-05-27 | 2021-08-27 | 华中农业大学 | Monascus monooxygenase mutant and application thereof |
| CN113423836A (en) * | 2019-01-11 | 2021-09-21 | 龙沙有限公司 | Production of carbon source regulated proteins in recombinant host cells |
-
2022
- 2022-08-01 CN CN202210917863.XA patent/CN115716868B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008023469A1 (en) * | 2006-08-25 | 2008-02-28 | Osaka University | Method of identifying transcriptional factor inducing the production of monascus-origin physiologically active substance and utilization thereof |
| CN113423836A (en) * | 2019-01-11 | 2021-09-21 | 龙沙有限公司 | Production of carbon source regulated proteins in recombinant host cells |
| CN113308443A (en) * | 2021-05-27 | 2021-08-27 | 华中农业大学 | Monascus monooxygenase mutant and application thereof |
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| Title |
|---|
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| "Dynamic regulation of Monascus azaphilones biosynthesis by the binary MrPigE-MrPigF oxidoreductase system";Yali Duan 等;《Appl Microbiol Biotechnol》;第106卷(第22期);第7519-7530页 * |
| "Monasone Naphthoquinone Biosynthesis and Resistance in Monascus Fungi";Mu Li 等;《mBio》;第11卷(第1期);doi: 10.1128/mBio.02676-19 * |
| Wang,Y.等."transcription factor [Monascus purpureus]".《genbank》.2019,ACCESSION QGA67219. * |
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