CN115710603B - Method for detecting CNV (complementary factor v) mark of INPP5E gene of merino sheep in high mountain and application - Google Patents
Method for detecting CNV (complementary factor v) mark of INPP5E gene of merino sheep in high mountain and application Download PDFInfo
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Abstract
The invention belongs to the technical field of molecular biology detection, and particularly relates to a method for detecting a CNV (carbon nanotubes) mark of an INPP5E gene of a merino alpine and application thereof, wherein the CNV mark related to the merino alpine wool character is positioned in an exon region chr3 of the INPP5E gene of the merino alpine: 2926901-2927900. And provides a method of detecting a CNV marker: the method is characterized in that the genome DNA of the high mountain merino sheep is used as a template, the primer pair P1 and P2 are used for amplifying the CNV region of the INPP5E gene and the internal reference gene through qPCR respectively, and the copy number variation of the INPP5E gene of the high mountain merino sheep is determined. Finally, the invention can detect CNV marks closely related to the wool traits of the mountain merino sheep, is used for the early selection of the mountain merino sheep, improves the seed selection accuracy, shortens the cultivation period and accelerates the cultivation process.
Description
Technical Field
The invention belongs to the technical field of molecular biology detection, and particularly relates to a method for detecting a CNV marker of an INPP5E gene of merino sheep in high mountain and application thereof.
Background
Wool is a layer of textile-value fiber which is covered on the surface of sheep body and is a derivative of skin. Wool is a main raw material of the wool spinning industry, and accounts for about 97% of the wool spinning raw material, and is mainly used for processing products such as clothing fabrics, knitting wool, blanket and the like. In wool production and breeding practice, wool characteristics are important economic characteristics, and mainly comprise a plurality of indexes such as wool length, wool yield, average fiber diameter, bending degree, breaking strength, elongation, net wool rate and the like, and are closely related to woven products and economic benefits. As the population grows, the demand for wool has exceeded supply. With the change of market demands, the production of high-quality wool has become a main goal of goat breeding.
The alpine merino sheep is a new merino sheep variety which takes Gansu alpine fine wool sheep as a female parent and Australian merino sheep as a male parent, comprehensively utilizes modern advanced biotechnology and breeding technology, adapts to alpine arid ecological regions with the altitude of 2400-4070 m in the first example of the world bred for 20 years, and has the wool fiber diameter of 19.0-21.5 mu m. Wool shape is also an important index for determining economic value, and objective inspection of wool shape is increasingly paid attention to wool sales, and is more and more tightly combined with spinning performance, breeding and production.
Compared with the traditional breeding method, the molecular marker assisted selection method has a plurality of advantages, such as obviously shortening the generation interval, improving the selection accuracy, advancing the selection time, and simultaneously having good selection effect on the characteristics of low genetic strength, the characteristics which are not represented in early stage, the characteristics which are difficult to measure in living bodies or have larger measurement difficulty and higher cost. Copy number variation (Copy Number Variations, CNVs), a newly discovered type of genomic sub-microscopic structural variation, refers to the deletion or duplication of larger fragments in genomic DNA, involving fragments ranging in size from 50bp to several Mb, including Copy number increase (Copy number gain) and Copy number decrease (Copy number loss).
The detection methods commonly used for CNV are largely divided into two categories, detection of unknown CNV over the whole genome and for site-directed detection or validation of known CNV. Common detection methods for unknown CNV of genome include chip method and sequencing method,however, both methods are limited by the detection platform and are expensive; for the detection of established CNV, methods based on PCR techniques and hybridization techniques are generally employed. Among them, real-time fluorescent Quantitative PCR (QPCR) is most commonly used. QPCR is performed by relatively quantifying a target gene (having copy number variation) and a reference gene (having no copy number variation), according to 2 -ΔΔCt The method is used for counting and detecting the copy number of the sample candidate genes. The method has the advantages of simple operation, high universality, high speed and high acceptance degree.
Inositol polyphosphate 5-phosphatase encoded by inositol polyphosphate 5-phosphatase gene (inositol polyphosphate-5-phosphotase, inp 5E) exists at the positions of cytoplasm, cytoskeleton, ciliated axial filament, golgi apparatus membrane, peripheral membrane protein and the like, and studies indicate that inp 5E regulates many cell activities including vesicle transport, cytoskeletal dynamics, protein synthesis, proliferation and survival and the like by regulating phosphoinositide signal. However, at present, no literature report on the research on the correlation between INPP5E gene CNV and sheep wool traits is available.
Disclosure of Invention
The invention provides a method for detecting a CNV mark of an INPP5E gene of a merino alpine and application thereof, in particular to a method for detecting a CNV mark of an INPP5E gene of a merino alpine based on a QPCR technology and application thereof. The method specifically comprises the following steps:
in a first aspect, the present invention provides a CNV marker associated with the merino alpine wool net hair rate trait, said CNV marker being located in the merino alpine inp 5E gene exon region chr3:2926901-2927900.
In a second aspect, the present invention provides an application of the CNV-labeled reagent related to the merino alpine wool net rate trait in the first aspect in detecting merino alpine wool net rate; the reagent amplifies the CNV marker and the internal reference gene ANKRD1 of the first aspect.
Preferably, the amplification result is according to 2X 2 -ΔΔCt The method defines copy number variation types: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal type wool has a better net wool ratio than the deletion type wool and the insertion type wool.
Preferably, the reagent sets the CNV-labeled amplification primer pair P1:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3';
and an amplification primer pair P2 of the reference gene ANKRD 1:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3'.
In a third aspect, the invention provides an application of a reagent for detecting CNV markers related to the wool purification rate characters of the merino alpine sheep in the first aspect in early breeding of merino alpine sheep; the reagent amplifies the CNV marker and the internal reference gene ANKRD1 of the first aspect.
Preferably, the amplification result is according to 2X 2 -ΔΔCt The method defines copy number variation types: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal type wool has a better net wool ratio than the deletion type wool and the insertion type wool.
Preferably, the reagent comprises the CNV-labeled amplification primer pair P1:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3';
and an amplification primer pair P2 of the reference gene ANKRD 1:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3'.
In a fourth aspect, the present invention provides a primer pair for detecting a CNV marker associated with the merino wool net hair rate trait in alpine, the primer pair comprising the CNV-labeled amplification primer pair P1 of the first aspect described above:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3';
and an amplification primer pair P2 of the reference gene ANKRD 1:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3'.
In a fifth aspect, the present invention provides a kit for QPCR detection of CNV markers according to the first aspect above, the kit comprising a primer pair according to the fourth aspect above.
Preferably, the kit further comprisesTop Green qPCR SuperMix, deionized water, control samples.
In a sixth aspect, the present invention provides a method for detecting a CNV marker associated with the merino wool net rate trait in alpine, the method comprising the steps of:
respectively amplifying the CNV marker and the reference gene ANKRD1 of the first aspect by QPCR with genomic DNA of the merino alpine as a template;
according to 2X 2 -ΔΔCt Quantitative results of the method divide the copy number variation into three categories: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal type wool has a better net wool ratio than the deletion type wool and the insertion type wool.
Preferably, the CNV-labeled amplification primer pair P1 is:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3';
the amplification primer pair P2 of the internal reference gene ANKRD1 is as follows:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3'.
Preferably, the QPCR amplification system comprises:top Green qPCR SuperMix 10. Mu.L of each of the upstream and downstream primers was 0.4. Mu.L, 1. Mu.L of genomic DNA and 8.2. Mu.L of nucleic-free Water.
Preferably, the reaction procedure used for QPCR is:
(1) Pre-denaturation: 94 ℃ for 30s;
(2) Amplification reaction: 94 ℃ for 5s; then 58 ℃ for 15s;72 ℃,10s;45 cycles;
(3) Drawing a melting curve: 95℃for 5s, followed by 5s from 65℃to 95℃and +0.5℃.
By combining all the technical schemes, the invention has the advantages and positive effects that:
the invention provides a CNV marker related to wool purification rate characteristics of merino alpine sheep, which is positioned in an exon region chr3 of an merino alpine sheep INPP5E gene: 2926901-2927900;
the invention provides a method for detecting CNV markers, which uses blood whole genome DNA of alpine merino sheep as a template, respectively expands CNV regions of the alpine merino sheep INPP5E gene by a real-time fluorescent quantitative PCR method, and uses ANKRD1 genes as a reference, 2 multiplied by 2 -ΔΔCt Quantitative results of the method divide the copy number variation into three categories: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal wool has higher net wool rate and is superior to the deletion type and insertion type; the invention detects CNV mark closely related to the wool net rate character of the mountain merino sheep on the DNA level, and can be used as an important candidate molecular mark for the mark auxiliary selection of the wool net rate character of the mountain merino sheep;
according to the invention, CNV of the INPP5E gene of the merino alpine is taken as a candidate site, the copy number variation condition of the site in the merino alpine population is detected by a real-time fluorescent quantitative PCR technology, and the correlation analysis is carried out with important economic characters such as wool net hair rate and the like; if the copy number variation type of the INPP5E gene candidate locus is detected to be normal, the wool net hair rate is higher; the research of the gene CNV and the correlation analysis of the gene CNV and important economic characters of the alpine merino sheep are important, so that a theoretical basis can be provided for molecular breeding of the alpine merino sheep in China, the marker-assisted selection of the wool purification rate characters of the alpine merino sheep is facilitated, and the alpine merino sheep population with excellent genetic resources is quickly established.
The copy number variation detection method of the gene of the merino alpine can be used for early breeding of the merino alpine; the method for detecting the copy number variation of the INPP5E gene is accurate and reliable and is simple and convenient to operate; the detection of the copy number variation locus of the INPP5E gene provides a scientific basis for the molecular marker assisted selection of the merino alpine sheep.
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In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 amplification curve of the merino sheep INPP5E gene in alpine;
FIG. 2 dissolution profile of the INPP5E gene of merino sheep at high mountain;
FIG. 3 dissolution profile of reference gene ANKRD1.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1 detection of the CNV marker of the merino sheep INPP5E Gene from high mountain
1. Sample collection
The method comprises the steps of collecting 152 merino sheep blood samples with production performance records from a breeding technology popularization station of Gansu sheep, taking 5mL of each merino sheep vein blood sample into a blood collection tube added with EDTA-K2 anticoagulant, quickly mixing the blood samples uniformly after the blood sample collection is finished, placing the blood samples into a sampling box containing an ice bag for temporary storage, transporting the blood samples back to a laboratory, and freezing the blood samples at the temperature of minus 20 ℃ for preservation for DNA extraction. The record of each sheep wool character (body side wool length, wool fiber diameter, wool shearing amount and net wool rate) is provided by a Gansu province sheep breeding technology popularization station.
2. Main reagent and instrument
EDTA-K2 vacuum blood collection tubes were purchased from Jiangsu Yuli medical instruments Co., ltd; blood genome extraction kit was purchased from tiangen biochemical technology (beijing) limited; nanoDrop2000 spectrophotometer us Thermo Fisher Scientific company;top Green qPCR SuperMix from Beijing all gold Biotechnology Co., ltd; real-time quantitative fluorescent PCR instruments were purchased from Roche company.
3. Extraction of blood genomic DNA
Extracting genome DNA from blood sample by adopting a blood genome extraction kit of Tiangen biochemical technology (Beijing) limited company, and placing the extracted DNA under an ultraviolet spectrophotometer to detect the concentration and purity, wherein the concentration is more than 20 ng/mu L, OD260/OD280 and is between 1.7 and 1.9, thus meeting the experimental requirement, and storing at-20 ℃ for standby.
4. Primer design
Referring to the international sheep genome oar_v4.0 version 3 chromosome INPP5E gene sequence as a reference sequence, a pair of specific primers are designed in a CNV region (chr 3: 2926901-2927900) by using primer premier5.0 software, and meanwhile, partial fragments of an internal reference gene ANKRD1 are designed and amplified by adopting the same method, the sequence information of the primers is shown in table 1, and the primers are synthesized by Beijing qing department biotechnology Co.
TABLE 1qPCR primer information Table
5. Real-time quantitative PCR amplification
qPCR amplification System 20. Mu.L:top Green qPCR SuperMix 10. Mu.L of each of the upstream and downstream primers was 0.4. Mu.L, 1. Mu.L of genomic DNA and 8.2. Mu.L of nucleic-free Water.
PCR amplification procedure: pre-denaturation at 94℃for 30s; amplification reaction at 94℃for 5s,58℃for 15s,72℃for 10s,45 cycles; the melting curve was drawn at 95℃for 5s, from 65℃to 95℃for 5s, +0.5℃.
The primer is determined by drawing an amplification curve and a dissolution curve, and is suitable for fluorescent quantitative PCR analysis. Wherein the amplification curve is smooth, which indicates that the fluorescent quantitative PCR amplification system and conditions are proper (the result is shown in FIG. 1); the dissolution curves of the target gene and the reference gene have obvious single peaks at the same positions on the abscissa, which indicates that the amplified products are single (the results are respectively shown in FIG. 2 and FIG. 3).
6. Calculation of copy number variation
Each individual was amplified with primers for the gene of interest and the reference gene, respectively, and 3 technical replicates were set for each individual. According to 2X 2 -ΔΔCt The method performs copy number analysis. Wherein ΔΔct= (Ct gene of interest-Ct reference gene) Experimental group - (CT objective gene-CT reference gene) Control group The method comprises the steps of carrying out a first treatment on the surface of the The experimental group is an individual sample of whether copy number variation exists or not to be detected; the control group is a sample of individuals known to have no copy number variation, and may be selected from reference individuals in a resequencing assay. 2 -ΔΔCt The copy number of the objective gene of the experimental group is shown as a multiple of that of the control group according to 2X 2 -ΔΔCt The results are divided into three categories: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type.
Correlation analysis of INPP5E Gene CNV and wool traits
The correlation of the copy number variation site of the merino sheep INPP5E gene in alpine and wool traits was analyzed by using a general linear model in IBM SPSS Statistics software, and the differences between the data sets were tested by using LSD multiplex comparison, and the results were expressed as average value.+ -. Standard error. The data processing results are shown in Table 2, the copy number variation locus of the INPP5E gene of the mountain merino sheep has a remarkable correlation (p < 0.05) with the wool net hair rate, wherein the wool net hair rate of the normal type is remarkably higher than that of the insertion type.
TABLE 2 correlation analysis of copy number variation of merino sheep INPP5E Gene at high mountain and growth traits
Note that: the different lower case letters between the same row of data indicate significant differences (P < 0.05), and n indicates the number of individuals with the same copy number.
In a word, the results show that the copy number variation of the INPP5E gene affects wool properties of the alpine merino sheep, CNV detection is used for carrying out early selection of the wool purification rate of the alpine merino sheep, so that the cultivation period is shortened, the cultivation process is quickened, the breeding cost is reduced, and the method has high application value.
The foregoing is merely illustrative of specific embodiments of the present invention, and the scope of the invention is not limited thereto, but any modifications, equivalents, improvements and alternatives falling within the spirit and principles of the present invention will be apparent to those skilled in the art within the scope of the present invention.
Claims (4)
1. The application of the CNV marked reagent related to the wool net rate character of the high mountain merino sheep in detecting the wool net rate of the high mountain merino sheep or the early breeding of the high mountain merino sheep; the CNV marker related to the wool-purifying rate character of the mountain merino sheep is positioned in a 2926901-2927900 region of chromosome 3 of the International sheep genome oar_v4.0 version, wherein the region is an INPP5E gene exon region; amplifying a CNV marker and an internal reference gene ANKRD1 by the reagent;
the reagents include the CNV-labeled amplification primer pair P1:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3'; and
the amplification primer pair P2 of the internal reference gene ANKRD 1:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3';
amplification results according to 2X 2 -ΔΔCt The method defines copy number variation types: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal type wool net rate is significantly higher than the insertion type wool net rate.
2. A method for detecting a CNV marker associated with the merino wool net rate trait in alpine, said method comprising the steps of:
respectively amplifying CNV markers and internal reference genes ANKRD1 related to wool purification rate characters of the alpine merino sheep by QPCR by taking genomic DNA of the alpine merino sheep as a template; the CNV marker is located in the 2926901-2927900 region of chromosome 3 of International sheep genome oar_v4.0 version 3, which is the exon region of the INPP5E gene;
the amplification primer pair P1 of the CNV label is as follows:
upstream primer F1:5'-CCTGACCTCCCCGCGCCATG-3';
downstream primer R1:5'-TCAAACGGTGAACCAGGATGTGC-3';
the amplification primer pair P2 of the internal reference gene ANKRD1 is as follows:
the upstream primer F2:5'-TCTTGTACCGATTCAGCC-3';
downstream primer R2:5'-TTCACTCGTTTATTGGGAT-3';
according to 2X 2 -ΔΔCt Quantitative results divide copy number variation into three categories: 2X 2 -ΔΔCt =2 is normal, 2×2 -ΔΔCt >2 is insertion type, 2×2 -ΔΔCt <2 is a deletion type; the normal type wool net rate is significantly higher than the insertion type wool net rate.
3. The method of claim 2, wherein the amplification system used for QPCR comprises: tranTop Green qPCR SuperMix 10. Mu.L each of the upstream and downstream primers was 0.4. Mu.L, 1. Mu.L of genomic DNA and 8.2. Mu.L of nucleic-free Water.
4. The method of claim 2, wherein the QPCR is performed using the following reaction procedure:
(1) Pre-denaturation: 94 ℃ for 30s;
(2) Amplification reaction: 94 ℃ for 5s; then 58 ℃ for 15s;72 ℃,10s;45 cycles;
(3) Drawing a melting curve: 95℃for 5s, followed by from 65℃to 95℃and +0.5℃.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110079615A (en) * | 2019-06-10 | 2019-08-02 | 西北农林科技大学 | A kind of method and its application of detection tea card sheep KMT2D gene C NV label |
| CN112342301A (en) * | 2020-11-12 | 2021-02-09 | 扬州大学 | Method for detecting Hu sheep NSMF gene CNV marker and application thereof |
| CN113416790A (en) * | 2021-08-14 | 2021-09-21 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing clean wool rate of alpine merino sheep and application thereof |
| CN114214426A (en) * | 2021-12-16 | 2022-03-22 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing alpine merino wool length character and application thereof |
| CN114214428A (en) * | 2021-12-16 | 2022-03-22 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing mohair shearing amount of alpine merino and application thereof |
| LU500801B1 (en) * | 2020-09-22 | 2022-03-25 | Inst Of Animal Sciences Of Chinese Academy Of Agricultural Sciences | Snp marker related to wool traits of fine-wool sheep, and detection primer set, kit, detection method and use thereof |
| CN114657266A (en) * | 2022-04-14 | 2022-06-24 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker for identifying haircut amount of whole-year-old alpine merino sheep and application thereof |
| CN114752680A (en) * | 2022-04-14 | 2022-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | SNP marker influencing diameter of alpine merino sheep wool fiber and application thereof |
| CN114752681A (en) * | 2022-04-14 | 2022-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | SNP marker influencing high-mountain merino sheep wool length and application thereof |
| CN114790483A (en) * | 2022-05-30 | 2022-07-26 | 中国农业科学院兰州畜牧与兽药研究所 | SNP locus combination related to wool cleaning rate of fine wool sheep and application thereof |
-
2022
- 2022-12-09 CN CN202211581867.1A patent/CN115710603B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110079615A (en) * | 2019-06-10 | 2019-08-02 | 西北农林科技大学 | A kind of method and its application of detection tea card sheep KMT2D gene C NV label |
| LU500801B1 (en) * | 2020-09-22 | 2022-03-25 | Inst Of Animal Sciences Of Chinese Academy Of Agricultural Sciences | Snp marker related to wool traits of fine-wool sheep, and detection primer set, kit, detection method and use thereof |
| CN112342301A (en) * | 2020-11-12 | 2021-02-09 | 扬州大学 | Method for detecting Hu sheep NSMF gene CNV marker and application thereof |
| CN113416790A (en) * | 2021-08-14 | 2021-09-21 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing clean wool rate of alpine merino sheep and application thereof |
| CN114214426A (en) * | 2021-12-16 | 2022-03-22 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing alpine merino wool length character and application thereof |
| CN114214428A (en) * | 2021-12-16 | 2022-03-22 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker influencing mohair shearing amount of alpine merino and application thereof |
| CN114657266A (en) * | 2022-04-14 | 2022-06-24 | 中国农业科学院兰州畜牧与兽药研究所 | SNP molecular marker for identifying haircut amount of whole-year-old alpine merino sheep and application thereof |
| CN114752680A (en) * | 2022-04-14 | 2022-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | SNP marker influencing diameter of alpine merino sheep wool fiber and application thereof |
| CN114752681A (en) * | 2022-04-14 | 2022-07-15 | 中国农业科学院兰州畜牧与兽药研究所 | SNP marker influencing high-mountain merino sheep wool length and application thereof |
| CN114790483A (en) * | 2022-05-30 | 2022-07-26 | 中国农业科学院兰州畜牧与兽药研究所 | SNP locus combination related to wool cleaning rate of fine wool sheep and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 甘肃高山细毛羊优质毛品系微卫星标记与经济性状的相关性分析;雒林通;杨博辉;史兆国;郭健;郎侠;岳耀敬;孙晓萍;;江苏农业学报(第02期);全文 * |
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