CN115678766A - Culture equipment and anti-condensation method thereof - Google Patents
Culture equipment and anti-condensation method thereof Download PDFInfo
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- CN115678766A CN115678766A CN202211394873.6A CN202211394873A CN115678766A CN 115678766 A CN115678766 A CN 115678766A CN 202211394873 A CN202211394873 A CN 202211394873A CN 115678766 A CN115678766 A CN 115678766A
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Abstract
A culture device and an anti-condensation method belong to the technical field of intelligent control. According to the culture equipment and the anti-condensation method provided by the invention, all the heaters arranged outside all the walls of the culture equipment are controlled by one temperature sensor in the culture chamber, so that all the heaters work simultaneously in one heating control period, but different finishing times are set according to different surface working conditions of the culture chamber (such as resistance values and installation position errors of the heaters, air speed difference of the inner wall of the culture chamber, heat insulation material difference of all the walls of the culture chamber and the like), the problem that the heaters are influenced mutually due to environmental temperature change when being controlled independently is solved, and condensation is prevented.
Description
Technical Field
The invention provides a culture device and an anti-condensation method thereof, and belongs to the technical field of intelligent control.
Background
Carbon dioxide is a colorless and odorless gas of which the aqueous solution is slightly sour, and is also a common greenhouse gas in air components, carbon dioxide is mainly applied to refrigeration of perishable foods (solid state), refrigerant (liquid state), production of carbonized soft drinks (gas state), solvent (supercritical state) for homogeneous reaction and the like, and a carbon dioxide incubator provides an optimal growth environment for cells by maintaining a humid atmosphere with a certain temperature and carbon dioxide concentration.
The precise control of the carbon dioxide concentration is crucial to maintain the pH stability of the cell growth environment, and is a key factor for the success of cell culture. The existing carbon dioxide concentration sensor has two types of thermal conductivity and infrared, but the measurement accuracy of the thermal conductivity sensor is greatly influenced by humidity and temperature. Therefore, infrared sensors are preferred. In order to maintain the sterile environment in the incubator, the inside of the incubator needs to be periodically sterilized, and high-temperature sterilization has been widely used because of its excellent sterilization effect. However, the high temperature resistant infrared sensor is expensive, and the high temperature environment inevitably affects the life span and measurement accuracy of the sensor. In addition, the particles have an influence on the measurement accuracy of the infrared sensor, so that the cleanliness of the measurement atmosphere must be controlled. It is therefore of particular importance to design a measurement system that can both control the amount of particles in the gas and avoid the effects of high temperatures. The carbon dioxide incubator is maintained at a high humidity of about 95% RH. The dew point temperature in such a high humidity environment is high and close to the culture temperature, so that the temperature of the inner wall and the inner door glass of the culture chamber cannot be lower than the dew point temperature, otherwise, dew condensation occurs. The water will grow bacteria and cross-contamination will occur, leading to cell culture failure. The temperature of the incubator is provided by heaters on the outer wall of the incubator chamber and an outer door, and the operation of the heaters is controlled by temperature sensors. Heaters are respectively arranged on 5 surfaces of the outer wall of the culture chamber and the surface of the outer door, the heaters are respectively and independently controlled by a plurality of temperature sensors, the most common is 3-way independent temperature control, the outer door temperature sensor controls the temperature of the outer door, the inner chamber bottom temperature sensor controls the humidifying temperature, and the main heating temperature sensor controls the rest heaters. Because the space between the outer door and the box body is sealed by the sealing strips around the outer door, the heat of the outer door can be dissipated from the sealing strips around when the heat of the outer door is radiated to the glass of the inner door, and the temperature of the glass of the inner door can be influenced by the ambient temperature of the box body. When the environment temperature is high, the heat is less dissipated from the sealing strips, the temperature of the glass inner door is high, the heat acquired by the culture chamber from other heaters is reduced when the heat of the glass door is transferred to the culture chamber, the heat output of the other heaters is reduced by the controller, and the temperature of the inner wall of the culture chamber is reduced to form dew condensation; when the ambient temperature is low, heat can be more dissipated from the sealing strip, so that the temperature of the inner door glass is too low and dew condensation is caused.
Disclosure of Invention
The invention mainly aims to provide a culture device and an anti-condensation method thereof, wherein all heaters arranged outside each wall of the culture device are controlled by one temperature sensor in a culture chamber, so that all the heaters work simultaneously in a heating control period, but different finishing times are set according to different surface working conditions of the culture chamber (such as resistance values and installation position errors of the heaters, air speed differences of inner walls, heat insulation material differences and the like), the problem of mutual influence caused by environmental temperature change in independent control is solved, and condensation is prevented.
In order to achieve the above object, the present invention provides a culture apparatus provided with a culture chamber, at least two walls of which are provided with heaters outside, characterized in that the culture apparatus further comprises a carbon dioxide concentration detection structure for measuring the concentration of carbon dioxide in the culture chamber; a temperature sensor for measuring a temperature within the incubation chamber; a storage table storing resistance value data and heater installation position data of heaters installed on the outer walls of the culture chamber, material property, thickness and area data of the inner walls of the culture chamber, and air velocity data of the inner walls of the culture chamber; a processor configured to control the operation time of the heater provided on each outer wall of the cultivation room based on the temperature data provided from the temperature sensor, the carbon dioxide concentration, the resistance value data of the heater provided on each outer wall and the setting position data thereof, the material property, thickness and area data of each inner wall of the cultivation room, and the wind speed data of each inner wall of the cultivation room.
In order to achieve the purpose, the invention also provides an anti-condensation method for the culture equipment, the culture equipment is provided with a culture chamber, heaters are respectively arranged outside at least two walls of the culture chamber, and the working time of the heaters arranged on the outer walls is controlled according to temperature data, carbon dioxide concentration, resistance value data and arrangement position data of the heaters arranged on the outer walls, material property, thickness and area data of the inner walls of the culture chamber and air speed data of the inner walls of the culture chamber.
Compared with the prior art, the invention has the following beneficial effects:
all heaters arranged outside each wall of the culture equipment are controlled by one temperature sensor in the culture chamber, so that all the heaters work simultaneously in one heating control period, but different finishing times are set according to different surface working conditions of the culture chamber (such as resistance values of the heaters, installation position errors, inner wall air speed differences, insulation material differences and the like), the problem that mutual influence is caused by environmental temperature change in independent control is solved, and dew condensation is prevented.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of a culture apparatus according to the present invention;
FIG. 2 is a schematic diagram of a top view of the culture apparatus provided in the present invention;
FIG. 3 is a schematic view of a front view structure provided by the present invention
FIG. 4 isbase:Sub>A schematic cross-sectional view taken along line A-A of FIG. 2;
FIG. 5 is a schematic cross-sectional view taken along line B-B of FIG. 2;
FIG. 6 is a block diagram showing the components of a control system of the cultivation apparatus according to the present invention.
In the drawings, the reference numbers indicate the following list of parts:
1. a culture chamber; 2. a partition plate; 3. an air suction connecting pipe; 4. an exhaust pipe; 5. a gas filter; 6. an air pump; 7. taking over the pipe; 8. an air inlet pipe; 9. an infrared carbon dioxide concentration sensor; 10. a circulating fan; 11. a conveying connecting pipe; 12. a carbon dioxide delivery pipe; 13. a concentration measuring cylinder; 14. an intake valve; 15. a connecting flange; 16. a support plate; 17. supporting the ribbon board; 18. and a caster.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In describing the present invention, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. The terms "comprises" and/or "comprising," when used in this specification, are intended to specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The term "and/or" includes any and all combinations of one or more of the associated listed items. The specific meanings of the above terms in the present invention can be understood in a specific case to those of ordinary skill in the art.
While exemplary embodiments are described as performing an exemplary process using a plurality of modules, it will be appreciated that the exemplary process may also be performed by one or more units.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
FIG. 1 is a schematic view of the overall structure of a culture apparatus according to the present invention; FIG. 2 is a schematic diagram of a top view of the culture apparatus provided in the present invention; FIG. 3 is a schematic view of a front view structure provided by the present invention; FIG. 4 isbase:Sub>A schematic cross-sectional view taken along line A-A of FIG. 2; FIG. 5 is a schematic cross-sectional view taken at B-B in FIG. 2;
as shown in FIGS. 1 to 5, the culture apparatus provided by the present invention comprises a culture chamber 1 and a partition plate 2 fixedly arranged on the inner wall of the culture chamber 1, wherein the partition plate 2 comprises a side plate part and a bottom plate part which are L-shaped, and a ventilation notch is arranged on the top surface of one side of the bottom plate part, the partition plate 2 divides the inner space of the culture chamber 1 into a culture chamber and an air duct chamber, the air duct chamber is arranged outside the culture chamber, and the culture chamber 1 is provided with a circulation convection mechanism.
According to the invention, the culture equipment comprises a carbon dioxide supply mechanism, the carbon dioxide supply mechanism comprises a conveying connecting pipe 11 and a carbon dioxide conveying pipe 12, the conveying connecting pipe 11 is embedded in the top surface of the culture chamber 1 and extends to an air channel chamber, the top end of the conveying connecting pipe 11 is sleeved with the carbon dioxide conveying pipe 12, the carbon dioxide conveying pipe 12 is sequentially provided with a concentration measurement interface 13 and an electric control air inlet valve 14, and the concentration measurement interface 13 is mainly convenient for personnel to detect the concentration of carbon dioxide introduced into the carbon dioxide conveying pipe 12 by virtue of external detection equipment; electronically controlled air inlet valve 14 is used to control the amount of air input to the incubation chamber. The end of the carbon dioxide conveying pipe 12 far away from the conveying connecting pipe 11 is provided with a connecting flange 15, which is convenient for connecting with a carbon dioxide gas supply container.
In the invention, the culture equipment also comprises a carbon dioxide concentration detection structure and a processor, wherein the carbon dioxide concentration detection structure is used for measuring the concentration of carbon dioxide in the culture chamber, the carbon dioxide concentration detection structure comprises a gas filter 5, a suction pump 6 and an infrared carbon dioxide concentration sensor 9, the suction pump 6 sucks gas from the culture chamber, the gas is filtered by the filter 5 and then is conveyed to the carbon dioxide concentration sensor 9, and the carbon dioxide concentration sensor 9 measures the concentration of carbon dioxide in the gas and then discharges the gas into the inner cavity of the culture chamber. Specifically, the carbon dioxide concentration detection structure comprises an exhaust tube 4, a gas filter 5, an exhaust pump 6, an infrared carbon dioxide concentration sensor 9 and an air inlet tube 8, wherein one end of the exhaust tube 4 is communicated with the culture chamber, the other end of the exhaust tube is communicated with an air inlet of the gas filter 5, an exhaust port of the gas filter 5 is connected to an air inlet of the exhaust pump 6 through a pipeline, an exhaust port of the exhaust pump is connected to an air inlet of the carbon dioxide concentration sensor 9, and an exhaust port of the carbon dioxide concentration sensor 9 is connected to the culture chamber through the air inlet tube 8. The carbon dioxide concentration sensor supplies the acquired carbon dioxide concentration to a processor of the culture apparatus. The gas filter 5 mainly filters and controls particulate impurities mixed in the carbon dioxide gas.
In the present invention, the cultivation apparatus further comprises a circulating convection mechanism including: the air inlet pipe 3, the circulating fan 10 and the connecting pipe 7 are arranged in the culture chamber 1, one end of the air suction connecting pipe 3 is embedded in the top surface of one side of the culture chamber, the bottom end of the air suction connecting pipe extends into the air duct cavity, the other end of the air suction connecting pipe is connected with the air inlet of the circulating fan 10, the other end of the circulating fan 10 is connected to one end of the connecting pipe 7, and the other end of the connecting pipe 7 is connected to the bottom of the air duct cavity; the circulation fan 10 is used to circulate the air in the cultivation room and the air duct chamber.
In the invention, a supporting plate 16 is fixedly arranged on the surface of one side of a culture chamber 1, two supporting strips 17 are fixedly arranged on the top surface of the supporting plate 16, a movable through hole is formed in one side of the top surface of each supporting strip 17, an air exhaust pipe 4 and an air inlet pipe 8 respectively and movably penetrate through one supporting strip 17, and the two supporting strips 17 respectively assist in fixing the air exhaust pipe 4 and the air inlet pipe 8.
In the invention, four caster wheels 18 are fixedly arranged at the corners of the bottom surface of the culture chamber 1, and the four caster wheels 18 are distributed in a rectangular array.
In the present invention, the outer surfaces of at least two walls of the culture chamber are provided with heaters, and preferably, the outer surfaces of the upper and lower walls, the front and rear walls, the left and right walls, and the outer door surface of the culture chamber of the culture apparatus are provided with heaters. Specifically, a 1 st heater is arranged outside the upper wall of the culture chamber, a 2 nd heater is arranged outside the front wall of the culture chamber, and a 3 rd heater is arranged outside the rear wall of the culture chamber; a 4 th heater is arranged outside the left wall of the culture chamber; the 5 th heater is arranged outside the right wall of the culture chamber.
According to one embodiment, aluminum foil is attached to both the culture chamber walls 17 and the heater surface.
In the present invention, the incubation apparatus is further provided with a temperature sensor, which is a probe extended to the incubation chamber, for measuring temperature information of the incubation chamber and supplying it to the processor.
FIG. 6 is a block diagram showing the components of a control system of the cultivation apparatus according to the present invention, and as shown in FIG. 6, the control system includes: a carbon dioxide concentration sensor for measuring a carbon dioxide concentration in the culture chamber; a temperature sensor for measuring a temperature within the incubation chamber; a memory table storing resistance value data and setting position data of each heater, material property, thickness and area data of each inner wall of the culture chamber, and wind speed data of each inner wall of the culture chamber; a processor configured to control the operation time of the heater based on the temperature data, the carbon dioxide concentration, the resistance value data of the heater and the setting position data thereof provided from the sensor, the material property, the thickness and the area data of each inner wall of the cultivation room, and the wind speed data of each inner wall of the cultivation room.
The invention also provides a method for preventing dewing of the culture equipment, the culture equipment is provided with a culture chamber, and heaters are arranged on the outer surface of the upper wall, the outer surface of the front wall, the outer surface of the upper wall, the outer surface of the lower wall and the surface of the outer door of the culture chamber.
In the invention, a corresponding relation table of the working power of the circulating fan and the wind speed of each inner wall of the culture chamber is also stored in the storage table, and the corresponding relation table is obtained by the following method: and each inner wall of the culture chamber is provided with an anemoscope for linearly adjusting the working power of the circulating fan, so that the wind speed of each inner wall of the culture chamber can not be obtained when the circulating fan has different working powers.
According to the invention, the processor at least comprises a wind speed pointer and an artificial intelligence module, wherein the wind speed pointer is configured to select wind speed data of each inner wall of the culture chamber corresponding to the working power to be provided for the artificial intelligence module according to the working power of the circulating fan; the artificial intelligence module comprises a data processing module, a feature extraction module and a deep learning module, and the data processing module processes information provided by the temperature sensor and the carbon dioxide concentration sensor to obtain a temperature time sequence and a carbon dioxide concentration time sequence; the characteristic extraction module is used for respectively extracting the characteristics of the temperature time sequence and the carbon dioxide concentration time sequence and then providing the extracted characteristics to the deep learning module; and the deep learning module determines the working time of the heater arranged on each outer wall according to the temperature time sequence, the carbon dioxide concentration time sequence and the wind speed of each inner wall of the culture chamber.
In the invention, the deep learning module comprises a neural network, the neural network comprises an input layer, a hidden layer, an output layer and a judgment layer,
the input layer input temperature time data sequence is X 1 ＝[x 11 … x n1 … x N1 ] T ；
The carbon dioxide concentration data series is: x 4 ＝[x 14 … x n4 … x N4 ] T ；
The wind speed data of the 1 st wall inner wall of the culture chamber is X 5 ＝[x 15 … x n5 … x N5 ] T ；
…
The M wall inner wind speed data sequence of the culture chamber is X M ＝[x 1M … x nM … x NM ] T ；
Deforming the temperature time data sequence to obtain a first matrix:
wherein,
x 13 ＝x 11 ；x n3 ＝f 1 (x n1 ) At room temperature of x n1 Of heat requiredA functional relationship; t is the time interval between adjacent measured temperatures; n is more than or equal to 3;
the first matrix, the carbon dioxide concentration data sequence, the wind speed data of the inner wall of the upper wall of the culture chamber, the wind speed data sequence in the front wall of the culture chamber and the wind speed data sequence in the rear wall of the culture chamber are combined; the wind speed data sequence in the left wall of the culture chamber and the wind speed data sequence in the right wall of the culture chamber form a second matrix:
wherein M is greater than or equal to 2, preferably M =6;
carrying out normalization processing on the second matrix by using a normalization coefficient matrix delta to obtain a third matrix:
Z＝I·δ
inputting each row of the normalized matrix into an input layer of the neural network, and activating neurons of a hidden layer of the neural network by using a Gaussian function to obtain a first vector:
Y＝[y n1 … y nk … y nK ]
r nm ＝f k (x nm )+y n(k-1) α k ，S nm is the center point of the Gaussian function, C nm Is the center point of the Gaussian function, w km Cross correlation coefficients of the k-th neuron of the hidden layer and the m-th neuron of the input layer; f. of k (x nm ) X for the k neuron data of the hidden layer and the m neuron input data of the input layer nm The functional relationship of (a); alpha (alpha) ("alpha") k Is a learning coefficient;
the output of the neurons of the output layer is represented by a second vector:
O nj ＝[q n1 … q nj … q nJ ]
in the formula
J is greater than or equal to 2,q nj Room temperature for the culture chamber is defined by (n-1)1 Change to x n1 The heat required to be provided by the jth heater on the outer wall of the culture chamber is needed; w is a jk Is the cross correlation coefficient between the j-th neuron of the output layer and the k-th neuron of the hidden layer. Preferably, J = M =6, i.e. one heater is provided on each outer wall of the culture chamber. Optionally, the culture apparatus has heaters disposed on the outer surfaces of the upper and lower walls, the front and rear walls, the left and right walls, and the outer door surface of the culture chamber, and J =7,m =6.
The output of the neuron that determines the slice is represented by a third vector: p = [ P ] 1 … p j … p J ]
In the formula,
alternatively,
ε j for the jth wall thermal coefficient of the culture chamber, I j Operating current of jth heater, R j Is the resistance value of the jth heater,
the time required to heat the growth chamber above the dew point temperature for all heaters; p is a radical of formula j When the number is 1, the jth heater of the culture chamber continues to work, and when the number is 0, the work is stopped;
then inputting the next group of data to the input layer by taking N as steps.
The control system provided by the invention also comprises an electric control valve control driver which drives the opening, the closing, the opening amount and the like of the electric control valve according to the control signal provided by the processor.
The present invention also provides a storage medium for storing a computer program for implementing the above-described method, wherein the storage includes permanent and non-permanent, removable and non-removable media, which may implement the storage of information by any method or technology. The information may be computer readable instructions, data structures, modules of a program, or other data. Memory includes, but is not limited to, phase change memory (PRAM), static Random Access Memory (SRAM), dynamic Random Access Memory (DRAM), other types of Random Access Memory (RAM), read Only Memory (ROM), electrically Erasable Programmable Read Only Memory (EEPROM), flash memory, compact disc read only memory (CD-ROM), digital Versatile Discs (DVD) or other optical storage, magnetic cassettes, magnetic tape storage or other magnetic storage devices, or any other non-transmission medium.
To achieve the object, the processor provided by the present invention is configured to execute a computer program, wherein the computer program is programmed to program the method or the steps of the method into program modules.
The temperature sensor can adopt an infrared temperature sensor which can be externally arranged, so that the influence of high temperature can be avoided; an air filter is arranged in the carbon dioxide concentration detection structure to remove particles, so that the measurement precision is ensured; the carbon dioxide concentration is uniformly dispersed in the whole space of the culture room through the circulating action of the fan and the air duct; according to the invention, the artificial intelligence module is used for repeatedly learning, so that the working time of all heaters arranged outside each wall of the culture equipment is determined according to the data provided by the temperature sensor in the culture chamber, the data provided by the carbon dioxide concentration sensor and the wind speed data of each inner wall of the culture chamber, the problem of mutual influence caused by environmental temperature change during independent control is solved, and dew condensation is prevented.
In the present invention, a new technical solution formed by various combinations of the above embodiments is also within the scope of the present disclosure.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. This need not be, nor should it be exhaustive of all embodiments. And obvious variations or modifications derived therefrom are intended to be within the scope of the invention.
Claims (10)
1. A culture apparatus provided with a culture chamber having heaters provided on the outer surfaces of at least two walls thereof, characterized by further comprising a carbon dioxide concentration detection structure for measuring the concentration of carbon dioxide in the culture chamber;
a temperature sensor for measuring a temperature within the incubation chamber;
a storage table storing resistance value data and heater installation position data of a heater installed on the outer wall of the culture chamber, material property, thickness and area data of each inner wall of the culture chamber, and air velocity data of each inner wall of the culture chamber;
a processor configured to control the operation time of the heater provided on each outer wall of the cultivation room based on the temperature data provided from the temperature sensor, the carbon dioxide concentration, the resistance value data of the heater provided on each outer wall and the setting position data thereof, the material property, thickness and area data of each inner wall of the cultivation room, and the wind speed data of each inner wall of the cultivation room.
2. The culture apparatus of claim 1, wherein the carbon dioxide concentration detecting structure comprises a gas filter, a suction pump and an infrared carbon dioxide concentration sensor, the suction pump draws gas from the culture chamber, and supplies the gas to the carbon dioxide concentration sensor after being filtered by the filter, and the carbon dioxide concentration sensor discharges the gas into the internal chamber of the culture chamber after measuring the concentration of carbon dioxide in the gas.
3. The cultivation apparatus according to claim 1 or 2, further comprising a circulation fan for circulating the gas in the cultivation room, wherein the storage table further stores a correspondence table between the operating power of the circulation fan and the wind speed of each inner wall of the cultivation room, the correspondence table being obtained by: and each inner wall of the culture chamber is provided with an anemoscope, and the working power of the circulating fan is linearly adjusted, so that the wind speed of each inner wall of the culture chamber is obtained when the circulating fan has different working powers.
4. The cultivation apparatus according to claim 3, wherein the processor comprises at least a wind speed indicator and an artificial intelligence module, the wind speed indicator being configured to select, according to the operating power of the circulation fan, to provide wind speed data of the inner walls of the cultivation room corresponding to the operating power to the artificial intelligence module; the artificial intelligence module comprises a data processing module, a feature extraction module and a deep learning module, and the data processing module processes information provided by the temperature sensor and the carbon dioxide concentration sensor to obtain a temperature time sequence and a carbon dioxide concentration time sequence; the characteristic extraction module is used for respectively extracting the characteristics of the temperature time sequence and the carbon dioxide concentration time sequence and then providing the extracted characteristics to the deep learning module; and the deep learning module determines the working time of the heater arranged on each outer wall according to the temperature time sequence, the carbon dioxide concentration time sequence and the wind speed of each inner wall of the culture chamber.
5. The culture apparatus of claim 4, wherein the deep learning module comprises a neural network comprising an input layer, a hidden layer, an output layer, and a discriminant layer,
the input layer input temperature time data sequence is X 1 ＝[x 11 …x n1 …x N1 ] T ；
The carbon dioxide concentration data sequence is: x 4 ＝[x 14 …x n4 …x N4 ] T ；
The wind speed data of the 1 st wall inner wall of the culture chamber is X 5 ＝[x 15 …x n5 …x N5 ] T ；
…
The M wall inner wind speed data sequence of the culture chamber is X M ＝[x 1M …x nM …x NM ] T ；
Deforming the temperature time data sequence to obtain a first matrix:
wherein x is 12 ＝0，
x 13 ＝x 11 ；x n3 ＝f 1 (x n1 ) At room temperature of x n1 The functional relationship of the heat required; t is the time interval between adjacent measured temperatures; n is more than or equal to 3;
the first matrix, the carbon dioxide concentration data sequence, the wind speed data of the inner wall of the upper wall of the culture chamber, the wind speed data sequence in the front wall of the culture chamber and the wind speed data sequence in the rear wall of the culture chamber are combined; the wind speed data sequence in the left wall of the culture chamber and the wind speed data sequence in the right wall of the culture chamber form a second matrix:
wherein M is greater than or equal to 2;
carrying out normalization processing on the second matrix by using a normalization coefficient matrix delta to obtain a third matrix:
Z＝I·δ
inputting each row of the normalization matrix into an input layer of the neural network, and activating neurons of a hidden layer of the neural network by using a Gaussian function to obtain a first vector:
Y＝[y n1 …y nk …y nK ]
r nm ＝f k (x nm )+y n(k-1) α k ，S nm is the center point of the Gaussian function, C nm Is the center point of the Gaussian function, w km For the k-th neuron of the hidden layer and the m-th neuron of the input layerA cross-correlation coefficient; f. of k (x nm ) X for the k neuron data of the hidden layer and the m neuron input data of the input layer nm The functional relationship of (a); alpha is alpha k Is a learning coefficient;
the output of the neurons of the output layer is represented by a second vector:
Q nj ＝[q n1 …q nj …q nJ ]
in the formula
J is greater than or equal to 2,q nj Room temperature for the culture chamber is defined by (n-1)1 Change to x n1 The heat required to be provided by the jth heater on the outer wall of the culture chamber is needed; w is a jk Is the cross correlation coefficient between the j-th neuron of the output layer and the k-th neuron of the hidden layer;
the output of the neuron that determines the slice is represented by a third vector: p = [ P ] 1 …p j …p J ]
In the formula,
ε j for the jth wall thermal coefficient of the culture chamber, I j Is the operating current of the jth heater, R j Is the resistance value of the jth heater,
the time required to heat the growth chamber above the dew point temperature for all heaters; p is a radical of j When the number is 1, the jth heater of the culture chamber continues to work, and when the number is 0, the work is stopped;
then inputting the next group of data to the input layer by taking N as steps.
6. The culture apparatus according to claim 5, wherein heaters are provided on outer faces of upper and lower walls, outer faces of front and rear walls, and outer faces of left and right walls of the culture chamber of the culture apparatus, and J = M =6; or the outer surfaces of the upper and lower walls, the front and rear walls, the left and right walls and the outer door surface of the culture chamber of the culture apparatus are provided with heaters, and J =7,M =6.
7. A method for preventing dew condensation in a culture apparatus having a culture chamber and heaters provided on the outer surfaces of at least two walls of the culture chamber, characterized in that the operation time of the heaters provided on the outer walls of the culture chamber is controlled based on temperature data provided by a temperature sensor, carbon dioxide concentration, resistance value data of the heaters provided on the outer walls and data of the positions where the heaters are provided, material properties, thickness and area data of the inner walls of the culture chamber, and wind speed data of the inner walls of the culture chamber.
8. The culture apparatus dewing prevention method according to claim 7, wherein the operating time of the heater is determined by an artificial intelligence module, and the processor comprises at least a wind speed indicator and the artificial intelligence module, wherein the wind speed indicator is configured to select, according to the operating power of the circulating fan, to provide wind speed data of each inner wall of the culture chamber corresponding to the operating power to the artificial intelligence module; the artificial intelligence module comprises a data processing module, a feature extraction module and a deep learning module, and the data processing module processes information provided by the temperature sensor and the carbon dioxide concentration sensor to obtain a temperature time sequence and a carbon dioxide concentration time sequence; the characteristic extraction module is used for respectively extracting the characteristics of the temperature time sequence and the carbon dioxide concentration time sequence and then providing the extracted characteristics to the deep learning module; and the deep learning module determines the working time of the heater arranged on each outer wall according to the temperature time sequence, the carbon dioxide concentration time sequence and the wind speed of each inner wall of the culture chamber.
9. The culture apparatus anti-dewing method according to claim 8, wherein the deep learning module comprises a neural network including an input layer, a hidden layer, an output layer and a judgment layer,
the input layer input temperature time data sequence is X 1 ＝[x 11 …x n1 …x N1 ] T ；
Carbon dioxide concentration numberAccording to the sequence: x 4 ＝[x 14 …x n4 …x N4 ] T ；
The wind speed data of the 1 st wall inner wall of the culture chamber is X 5 ＝[x 15 …x n5 …x N5 ] T ；
…
The M wall wind speed data sequence of the culture chamber is X M ＝[x 1M …x nM …x NM ] T ；
Deforming the temperature time data sequence to obtain a first matrix:
wherein x is 12 ＝0，
x 13 ＝x 11 ；x n3 ＝f 1 (x n1 ) At room temperature of x n1 The functional relationship of the heat required; t is the time interval between adjacent measured temperatures; n is more than or equal to 3;
the first matrix, the carbon dioxide concentration data sequence, the wind speed data of the inner wall of the upper wall of the culture chamber, the wind speed data sequence in the front wall of the culture chamber and the wind speed data sequence in the rear wall of the culture chamber are combined; the wind speed data sequence in the left wall of the culture chamber and the wind speed data sequence in the right wall of the culture chamber form a second matrix:
wherein M is greater than or equal to 2;
carrying out normalization processing on the second matrix by using a normalization coefficient matrix delta to obtain a third matrix:
Z＝I·δ
inputting each row of the normalization matrix into an input layer of the neural network, and activating neurons of a hidden layer of the neural network by using a Gaussian function to obtain a first vector:
Y＝[y n1 …y nk …y nK ]
r nm ＝f k (x nm )+y n(k-1) α k ，S nm is the center point of the Gaussian function, C nm Is the center point of the Gaussian function, w km Cross correlation coefficients of the k-th neuron of the hidden layer and the m-th neuron of the input layer; f. of k (x nm ) X for the k neuron data of the hidden layer and the m neuron input data of the input layer nm The functional relationship of (a); alpha (alpha) ("alpha") k Is a learning coefficient;
the output of the neurons of the output layer is represented by a second vector:
Q nj ＝[q n1 …q nj …q nJ ]
in the formula
J is greater than or equal to 2,q nj Room temperature for the culture chamber from x (n-1)1 Change to x n1 The heat required to be provided by the jth heater on the outer wall of the culture chamber is needed; w is a jk Is the cross correlation coefficient between the j-th neuron of the output layer and the k-th neuron of the hidden layer;
the output of the neuron of the fault is represented by a third vector: p = [ P ] 1 …p j …p J ]
In the formula,
ε j for the jth wall thermal coefficient of the culture chamber, I j Operating current of jth heater, R j Is the resistance value of the jth heater,
the time required to heat the growth chamber above the dew point temperature for all heaters; p is a radical of formula j When the number is 1, the jth heater of the culture chamber continues to work, and when the number is 0, the work is stopped;
then inputting the next group of data to the input layer by taking N as steps.
10. The dew condensation preventing method for a culture apparatus according to claim 10, wherein the culture apparatus has heaters provided on the outer surfaces of the upper and lower walls, the front and rear walls, and the left and right walls of the culture chamber, J = M =6, or heaters provided on the outer surfaces of the upper and lower walls, the front and rear walls, the left and right walls, and the outer door surface of the culture chamber, J =7,m =6.
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| CN117906684A (en) * | 2024-03-20 | 2024-04-19 | 上海博迅医疗生物仪器股份有限公司 | Measuring device of drying chamber and intelligent drying system of vacuum drying oven |
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| CN117906684A (en) * | 2024-03-20 | 2024-04-19 | 上海博迅医疗生物仪器股份有限公司 | Measuring device of drying chamber and intelligent drying system of vacuum drying oven |
| CN117906684B (en) * | 2024-03-20 | 2024-05-24 | 上海博迅医疗生物仪器股份有限公司 | Measuring device of drying chamber and intelligent drying system of vacuum drying oven |
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