CN115669400A - Method for evaluating salt tolerance of plants - Google Patents
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Abstract
The invention relates to the field of plant planting, in particular to a method for evaluating salt tolerance of plants. The method comprises the following steps: (1) Measuring the biomass percentages of a plurality of plants respectively corresponding to different salt concentrations; (2) Respectively drawing a curve chart of each plant according to the data in the step (1) by taking the salt concentration as an abscissa and the biomass percentage as an ordinate, and respectively measuring and calculating the area of a region enclosed by the curve chart of each plant and an x axis and a y axis, namely the biomass area S of the plant i (ii) a (3) Determination of reference plant X b And other plants are marked as X i (ii) a With reference to plant X on the abscissa b When the biomass percentage of (2) is 0%, the point of the corresponding salt concentration, the point of the biomass percentage of 100%, and the origin are taken as three endpoints of a rectangle, and a reference rectangle is drawn, wherein the area of the reference rectangle is S; (4) Calculating the salt tolerance index T of each plant i ＝S i and/S. The invention is simpler and more convenient, has lower cost and has more practical operability.
Description
Technical Field
The invention relates to the field of plant planting, in particular to a method for evaluating salt tolerance of plants.
Background
Soil salinization is a worldwide ecological environment problem. According to incomplete statistics, the salinized land all over the world accounts for about 10% of the total land area, and the salinized land in China is close to 1 hundred million hectares. The salinization of the land not only influences agricultural production and land development and utilization, but also poses serious threats to ecological safety and urban and rural environment improvement, and becomes a significant limiting condition and barrier factor for restricting the sustainable development of the economy and the society. With the increasing population, economic development and increasing urbanization level, the pressure on resource environment, particularly cultivated land and ecological land, is larger and larger, and the status and value of the saline-alkali soil as an important backup land resource and ecological land are increasingly highlighted.
The problem is solved mainly in two aspects: firstly, selecting plants with higher salt tolerance; and secondly, the salt tolerance of the plants is gradually improved through cultivation and screening. In both aspects, a more convenient and accurate quantitative evaluation method for the salt tolerance of the plants is needed.
The current evaluation method for plant salt tolerance, such as quantitative evaluation technology for plant salt tolerance, comprises the following steps: 1. selecting plant individuals with the same specification and representative container planting, and forming soil salt stress gradient by each container by using different watering days. And when the soil salt is stressed to a certain degree, determining the soil salt concentration of each container and the damage degree of the cell membrane of the corresponding plant, wherein the change degree of the permeability of the plant cell membrane is determined by using a plant activity determinator. And then fully irrigating, and measuring the change trend of the restoration capability of each plant cell membrane after 48 hours, thereby finding out the critical point where the plant cell membrane is slowest to restore and cannot be restored, wherein the original soil water potential corresponding to the critical point is the salt tolerance limit value of the plant. The value is used as an index for quantitative evaluation of the salt resistance of the plant.
However, the prior art method for measuring the salt tolerance of plants has the following problems: the need to resort to specific technical equipment, both manual and technical, is costly, complex or difficult to operate, and the results are not obvious.
Therefore, it is of great significance to research a simpler, more accurate, nondestructive and more efficient evaluation method for plant salt tolerance.
Disclosure of Invention
The present invention has been made in view of the above problems of the prior art, and an object of the present invention is to provide a method for evaluating salt tolerance of a plant. The method is simpler, lower in cost, more scientific and more practical in operation.
The inventor of the invention finds that the prior art and method usually need to determine the salt tolerance of plants by means of various indexes such as plant growth, form, physiology and biochemistry and even molecules and often need special technical equipment, such as the determination of physiological and biochemical indexes such as plant cell membrane permeability, photosynthesis, enzyme activity, proline, betaine and the like, and then by combining the indexes such as plant growth, form and the like, a membership function method is used for judging the salt tolerance of plants, and accordingly, the instrument cost and the technical cost are high; or the growth stage of the plant needs to be strictly divided into various periods in the prior art, and strict monitoring is carried out in the growth process; and the data of the plants are respectively obtained according to different time or different positions, and then the data are substituted into a complex formula for calculation, so that the operation is complex, and the labor cost and the time cost are high.
The inventor of the invention finds that the salt-alkali stress in the environment is finally expressed by the plant biomass, and in the actual operation, the absolute parameters of complex indexes such as plant physiology and biochemistry and salt tolerance data do not need to be strictly measured, but only relative parameters for guiding the experimental direction are needed, unless special needs exist. By setting a proper calculation method of relative parameters, the method can effectively save instrument cost, technical cost, labor cost and time cost, simply, efficiently and scientifically evaluate the salt tolerance of the plants, and rapidly guide the further progress of the experiment. Thus, the inventors of the present invention have intensively studied to find the evaluation method of the present invention.
In order to achieve the above object, the present invention provides a method for evaluating salt tolerance of a plant, the method comprising the steps of:
(1) Measuring the biomass percentages of a plurality of plants respectively corresponding to different salt concentrations;
(2) Respectively drawing a curve chart of each plant according to the data in the step (1) by taking the salt concentration as an abscissa and the biomass percentage as an ordinate, and respectively measuring and calculating the area of a region enclosed by the curve chart of each plant and an x axis and a y axis, namely the biomass area S of the plant i ；
(3) Determination of reference plant X b And other plants are marked as X i (ii) a Calculating the reference area S as: on the abscissa the reference plant X b When the biomass percentage of (3) is 0%, drawing a reference rectangle having an area of S, wherein the three endpoints of the rectangle are a point of the corresponding salt concentration, a point of the biomass percentage of 100%, and an origin;
(4) Calculating the salt tolerance index T of each plant i ＝S i (iv)/S, wherein i is a positive integer selected from 1, 2 … … b, representing a different plant species or genotype, wherein b represents a reference plant.
Based on the method, the salt tolerance index T of each plant can be obtained i Based on the T i The relationship of salt tolerance of each plant can be judged. Note that, because of T i Is based on a reference plant X b Relative values of the base rectangular area S, and thus in different sets of experimentsObtained T i There is no comparison between them, unless the same reference plant b and reference area S are used. But the salt tolerance index T i The method is enough to guide technicians to judge whether the plant to be evaluated has enough salt tolerance or not, and proves that the salt tolerance of the plant to be evaluated is strong or weak. In addition, obviously, the method only needs to measure the biomass percentages of a plurality of plants respectively corresponding to different salt concentrations, and has the advantages of simple and conventional operation mode, low cost of manpower, technology and instruments and high accuracy.
The salt concentration is the abscissa of the biomass linear equation. In the present invention, the term "salt concentration" is the concentration of sodium salt in the soil used in the experiment, and the unit is not strictly limited because the unit is offset when calculating Ti, for example, the unit can be selected from mM, i.e., mmol/L.
In the present invention, the sodium salt includes various sodium salt forms which may exist in the soil, such as NaCl, na 2 SO 4 、Na 2 CO 3 And NaHCO 3 One or more of (a).
In the present invention, the "salt concentration" is calculated as the concentration of NaCl, that is, the amount of NaCl uniformly converted from each sodium salt.
In carrying out the experiment, the salt concentration may be obtained by active formulation, for example, including: 1. determining the salt application concentration (mmol/L) and the soil water holding capacity (L); 2. shi Yanliang (mmol) was calculated according to the formula "salt concentration (mmol/L) = Shi Yanliang (mmol) ÷ soil water holding capacity (L)"; 3. calculating the mass of NaCl to be applied according to the relative molecular mass of 58.44g/mol of NaCl; 4. NaCl was applied daily in increasing concentration gradients until the desired salt concentration was reached, increasing the salt concentration no more than 150mmol/L daily for no more than 10 days of stepwise application.
The biomass percentage is the ordinate of the biomass linear equation. In the present invention, the term "percentage biomass" is generally a set of data from 0% to 120% (and therefore higher than 100% because some species of plants have growth promoting phenomena at low salt concentration and biomass higher than 0 salt concentration), for each plant, the biomass reached by normal growth of the plant (i.e. no salt control) corresponds to the position on the ordinate "percentage biomass is 100%" when the salt concentration is 0, the amount of growth gradually decreases when the salt concentration increases, and corresponds to "percentage biomass is 0% when no plant is grown at all"; in this process, the ratio of the biomass obtained by the plants under salt stress relative to the biomass of the salt-free control corresponds to the value of the percentage of biomass on the ordinate. That is, 100% of the points on the ordinate of all plants coincide, and the value on the ordinate corresponding to each plant stressed with different salt concentrations is the relative value based on the own salt-free control biomass.
In step (1), a scatter plot is obtained in the coordinate graph by setting multiple sets of parallel tests to obtain the biomass percentage corresponding to each plant under multiple salt concentrations.
In step (1), the method for determining the biomass percentage of plants corresponding to different salt concentrations can be performed according to the conventional manner in the field, for example, the method comprises the following steps: and (3) drying the plant to be detected at 85 ℃ until the quality is constant, naturally cooling, and weighing the dry weight of the plant.
In step (2), the method for measuring the biomass area can be any method capable of calculating the area, such as function fitting method or direct measurement method.
According to a preferred embodiment, the method of measuring the biomass area is a function fitting method.
The function fitting method comprises fitting the graph to a function equation F (x) i ) And calculating the area of the area enclosed by the functional equation, the x axis and the y axis by adopting a calculus method according to the functional equation. The method of fitting a linear equation by a scatter plot is in a manner conventional in the art, e.g., using computer iteration methods, e.g., using software Excel, SPSS, sigmaplot, origin, graphpad, etc. Generally achieving a degree of fitting R 2 Not less than 0.9 can be used in the present invention, and R is preferably 2 Not less than 0.95, more preferably R 2 ≥0.98。
According to another preferred embodiment, the method for measuring the biomass area is a direct measurement, wherein the area of the region is measured directly using an area measuring tool.
The area measurement tool is for example CAD software. For example, by drawing the area to be measured in the CAD, the area can be directly read by the CAD measuring tool.
In the present invention, the term "biomass area" refers to the triangular-like area enclosed by the linear equation of biomass for each plant with the abscissa (salt concentration corresponding to 0 to zero biomass) and the ordinate (0 to 100%).
In the present invention, the numerical value of the subscript "i" itself has no meaning in that different plants are given different numbers i so that the plants can be easily distinguished from each other.
In the step (3), the reference plant X b The selection of (2) suggests adopting halophytes with the strongest salt tolerance. If a comparison is made between the test plants, X b For example, plants familiar to the skilled person may be selected; in a more preferred embodiment, however, the reference plant X b The plants with the strongest salt tolerance were evaluated. If it is uncertain which plant is most salt tolerant, the biomass linear equation F (x) of each plant can be obtained first i ) Then comparing, and selecting the most salt-tolerant plant as a reference plant X b 。
In another specific embodiment, the reference plant X b The plants that were the least salt tolerant of the plants involved in the evaluation may also be used. The setting can be made as required by those skilled in the art.
Preferably, said reference plant X b Is a plant capable of tolerating a salt concentration of 600mM or more, more preferably 700mM or more, the salt concentration being the sodium salt concentration in NaCl.
Preferably, said reference plant X b Is halophyte, namely the plant with higher salt tolerance; for example selected from Nitraria tangutorum, tamarix chinensis, suaeda salsa and Salicornia europaea. For example, by selecting Nitraria tangutorum bobr as a reference plant, the standards can be unified between each batch of experiments, and the resulting tolerance can be obtainedSalt indexes are comparable to each other.
In step (3), by determining a reference plant X b And calculating a reference area S, wherein the S is the evaluation basis of the evaluation method. The reference plant X b For determining a reference rectangle from 0 point on the abscissa to the reference plant X b The line segment between the points of the salt concentration corresponding to the biomass percentage of (1) is one side of a reference rectangle, and points from 0 to 100% on the ordinate are the other side of the reference rectangle to form the reference rectangle, and the area of the reference rectangle is S.
In the step (4), the salt tolerance index of each plant is calculated by T i ＝S i and/S. The obtained Ti is the parameter for representing the salt tolerance of each plant. T is i The larger the size, the stronger the salt tolerance.
One skilled in the art can base on the T obtained i The salt tolerance of the plants is judged and selected according to the specific data and the actual conditions of the experiment.
For example, when the object is to select and continue to culture a plant variety with stronger salt tolerance, the T of the plant to be tested is measured according to the method of the invention i Respectively with a threshold index T n Comparing; for example, the salt tolerance index T of a plant is measured m When T is reached m ≥T n When the plant has ideal salt tolerance, the plant is selected; when T is m ＜T n When the salt tolerance of the plant is not enough, the plant is eliminated. Wherein the threshold index T n Set by researchers according to experimental requirements and specific conditions.
In the present invention, the method may further comprise step (5): calculating the relative salt tolerance index G of each plant i ＝T i /T b Wherein, T b ＝S b /S，S b Is the reference plant X b Biomass area of (1), G b ＝1。
By further calculating the relative salt tolerance index G i Enabling each plant to be directly used as a reference plant X b Based on the salt tolerance of (A), a relative value is obtained, namely G i In the determination of plantsRelative salt tolerance will be more intuitive.
The person skilled in the art is able to rely on the G obtained i The salt tolerance of the plants is judged and selected according to the specific data and the actual conditions of the experiment. Determination method and the above-mentioned T i Similarly.
When the aim is to select and continue to culture the plant variety with stronger salt tolerance, G is measured by the plant to be measured according to the method of the invention i Respectively with a threshold index G n Comparing; for example, the relative salt tolerance index G of a plant is measured m When G is present m ≥G n When it is, G is considered m Selecting the plants with ideal salt tolerance; when G is m ＜G n When it is, G is considered m The salt tolerance of the corresponding plants is insufficient, and the plants are eliminated. Wherein the threshold index G n Set by researchers according to experimental requirements and specific conditions.
In addition, the method of the invention can be used for judging the salt tolerance of the plant and can also be used for estimating the biomass of the plant under different salt concentrations. Thus, the method of the invention may further comprise, based on said fitted biomass linear equation F (x) i ) Substituting the salt concentration, calculating the biomass percentage of the plant at the salt concentration. The biomass of the plant at the salt concentration can be estimated by multiplying the percentage biomass by the biomass at the no salt control.
Through the technical scheme, compared with the prior art, the invention at least has the following advantages:
(1) The method is simple to operate, and has lower labor cost and technical cost;
(2) The method does not involve complicated regulation and control at each stage, particularly does not need to purposely determine the salt concentration of the matrix soil (or solution) for culturing the plants, and only takes the actually measured salt concentration of the matrix soil (or solution) as the standard, so the method has few influencing factors, is simple and has high result reliability;
(3) The method of the invention does not relate to complex operation and does not need to measure complex parameters (such as physiological and biochemical indexes of cell membrane damage degree) and only needs counting and calculation, thereby greatly reducing the labor cost, the technical cost, the instrument cost and the time cost;
(4) The salt tolerance parameter of the invention has more intuitive result and has more guiding significance in actual operation.
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
Drawings
FIG. 1 is a plot of the scatter plot, fitted linear curve and area of biomass percentage of Nitraria tangutorum under stress of different salt concentrations.
FIG. 2 is a plot of the percentage biomass of Elaeagnus angustifolia under stress at different salt concentrations, fitted linear curves and regions of biomass area.
FIG. 3 is a plot of the scatter of the percentage of biomass of Populus nigra under stress at different salt concentrations, a linear curve after fitting, and the area of biomass area.
Fig. 4 is a schematic region diagram of the reference area S.
Detailed Description
The present invention will be described in detail below by way of examples. The described embodiments of the invention are only some, but not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The evaluation method of the present invention is exemplarily illustrated by examples below.
The method is characterized in that nitraria tangutorum bobr and elaeagnus angustifolia are randomly selected as reference plants, and the salt tolerance of the nitraria tangutorum bobr and the elaeagnus angustifolia is evaluated by the method.
Examples
(1) Preparation of
Adopting peat: perlite =1:1 (volume ratio) mixture as a substrate, several white thorns (siberia white thorns are used in this example), black poplar and narrow-leaved oleaster were planted in plastic pots with trays, and regular maintenance management was performed periodically. Selecting nursery stocks with consistent growth vigor before the beginning of salt stress, and respectively setting three-pot parallel experiments for each salt gradient of each plant (averaging the results obtained by three pots in the test). In this example, 33 pots of Nitraria tangutorum, 27 pots of Elaeagnus angustifolia, and 12 pots of Populus nigra were prepared.
(2) Setting salt stress gradient
Single salt NaCl is adopted for stress treatment, and different stress gradients are set for different types of seedlings. Specifically, the following (unit: mmol/L) was set:
the white thorn is provided with 11 gradients of 0, 50, 100, 150, 200, 250, 300, 400, 500, 600 and 700;
setting oleaster with 9 gradients of 0, 50, 100, 150, 200, 250, 300, 400 and 500;
the black box was set at 4 gradients of 0, 50, 100, 150.
Each treatment was repeated 3 times (i.e. 3 pots of plants).
(3) Performing salt stress test
Step 1-preparation of salt stress environment: according to the concentration gradient set as described above, the salt concentrations applied to the three plants each day were determined so that they simultaneously reached the expected salt concentrations the day before the start of the salt stress experiment. Specifically, since the 700mmol/L gradient of the Nitraria tangutorum bobr requires 7 days to reach, the rate of increase in the concentrations of Populus nigra and Nitraria tangutorum bobr was set to reach the preset concentration on day 7. Tables 1, 2 and 3 are the amounts of salt concentration applied per day for each concentration gradient of nitraria tangutorum, oleaster and black poplar, respectively;
step 2-calculate the weight of NaCl specifically applied: water holding capacity of a culture pot (for example, water holding capacity of a large culture pot used in this experiment is 1.2L) × salt concentration (mmol/L) × 58.44 (relative molecular mass of NaCl 58.44 g/mol) = mass of NaCl applied daily (mg);
step 3-salt stress experiment: after the three plants all reached the preset salt concentration at day 7, salt stress experiments were performed starting from day 8; this day 8 was taken as the first day of the salt stress experiment, after which no longer NaCl was applied and normal care was applied to the plants at the existing salt concentration until day 21 of the salt stress experiment. ( During salt stress period, regular quantitative watering is carried out to balance evaporation amount and prevent and control plant diseases and insect pests. Plastic trays are arranged under the cultivation pots so as to pour the flowing NaCI solution back into the cultivation pots in time to prevent salt loss. )
Step 4-determination of percentage increase in biomass of whole plant: after 21 days of the salt stress experiment, taking down the whole plant, drying the whole plant at 85 ℃ until the quality is constant, naturally cooling the plant, weighing the dry weight of each plant, and averaging the dry weights of the three pots of plants under each salt stress concentration of each plant to obtain the biomass of the plant under the salt stress concentration.
Step 5-calculate biomass percentage: the ratio of biomass at each salt stress concentration divided by the biomass at the salt concentration of 0 was the percentage of biomass at that salt stress concentration.
Watch 1 (white thorn)
Table 2 (narrow-leaved oleaster)
Table 3 (Black poplar)
(4) Obtaining scatter diagram of different plant biomass percentages under stress of different NaCl concentrations
According to the results of the salt stress test, the biomass percentages (expressed in decimal) of Nitraria tangutorum, elaeagnus angustifolia and Populus nigra at different salt concentrations were obtained, and scatter plots were plotted with the salt concentration (in mM) as abscissa and the biomass percentage (in%) as ordinate, and the Nitraria tangutorum, elaeagnus angustifolia and Populus nigra were shown in FIG. 1, FIG. 2 and FIG. 3, respectively.
(5) Fitting biomass linear equation
From the results of step (4), SPSS was used to fit linear equations for biomass of Nitraria tangutorum bobr, elaeagnus angustifolia and Populus tremula, as shown in FIG. 1, FIG. 2 and FIG. 3, respectively. The resulting equations are respectively denoted as F (x) b )、F(x 1 ) And F (x) 2 ) Specifically, the following is made. x represents the sodium salt concentration (mmol/L) and F (x) represents the biomass (in decimal).
White thorn: f (x) b )＝-2×10 -13 x b 5 +4×10 -10 x b 4 -2×10 -7 x b 3 +3×10 -5 x b 2 -0.0009x b +1.0124，R 2 ＝0.9979；
Narrow-leaved oleaster: f (x) 1 )＝1×10 -8 x 1 3 -6×10 -6 x 1 2 -0.0022x 1 +1.0278，R 2 ＝0.9902；
Black poplar: f (x) 2 )＝2×10 -5 x 2 2 -0.0107x 2 +1.034，R 2 ＝0.9656。
It can be seen that the model fitting degree of the white thorn is as high as 0.9979, which indicates that the model can explain 99% of variation; the fitting degree of the oleaster model is as high as 0.9902, which shows that the model can explain 99% of variation; the model fitting degree of the populus nigra is as high as 0.9656, which shows that the model can explain the variation of 96%. As can be seen, the three plants can achieve very fitting degree, and the method provided by the invention is proved to have higher accuracy and practical operability.
(6) Determination of biomass area
The area of biomass was obtained by direct area measurement with CAD (fig. 4). Determining the white thorn as a reference plant O, wherein the schematic diagram of the area of the reference plant is shown in figure 4; a schematic of the area of the biomass area of the spinous processes is shown shaded in fig. 1 and in fig. 4; a schematic of the area of biomass area of elaeagnus angustifolia is shown shaded in fig. 2 and in fig. 4; the area of the biomass area of the black poplar is schematically shown in fig. 3 and shaded in fig. 4.
And (3) measuring:
reference area S =625.4 (relative amounts, without units, as follows);
biomass area S of Nitraria tangutorum bobr b ＝482.0；
Biomass area S of Elaeagnus angustifolia 1 ＝182.5；
Biomass area S of black poplar 2 ＝59.0。
(7) Calculating the salt tolerance index T i
Salt tolerance index T of nitraria tangutorum bobr 0 ＝S b ÷S＝0.77。
Salt tolerance index T of oleaster 1 ＝S 1 ÷S＝0.38；
Salt tolerance index T of black poplar 2 ＝S 2 ÷S＝0.12。
Therefore, the salt tolerance of the oleaster is higher than that of the black poplar, and the difference degree of the salt tolerance of the nitraria tangutorum bobr, the oleaster and the black poplar can be seen.
If a threshold index T is set n Is 0.2. Selecting narrow-leaved oleaster and eliminating black poplar.
(8) Calculating relative salt tolerance index G i
Relative salt tolerance index G of Elaeagnus angustifolia 1 ＝T 1 ÷T 0 ＝0.49；
Relative salt tolerance index G of black poplar 2 ＝T 2 ÷T 0 ＝0.16。
It can be seen that the salt tolerance of elaeagnus angustifolia is stronger than that of black poplar.
If a threshold index G is set n Is 0.3. Selecting oleaster and eliminating black poplar.
The method is simple to operate, and low in labor cost and technical cost; the method does not relate to complicated regulation and control of each stage, so that the influence factors are few, and the result reliability is high; the method of the invention does not relate to complex screening, weighing and the like, does not need to measure complex parameters (such as cell membrane damage degree), only needs counting and calculation, and reduces the labor cost, the technical cost, the instrument cost and the time cost; the salt tolerance parameter of the invention has more intuitive result and more guiding significance in actual operation.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. A method for evaluating salt tolerance of a plant, comprising the steps of:
(1) Measuring the biomass percentages of a plurality of plants respectively corresponding to different salt concentrations;
(2) Respectively drawing a curve chart of each plant according to the data in the step (1) by taking the salt concentration as an abscissa and the biomass percentage as an ordinate, and respectively measuring and calculating the area of a region enclosed by the curve chart of each plant and an x axis and a y axis, namely the biomass area S of the plant i ；
(3) Determination of reference plant X b And other plants are marked as X i (ii) a Calculating the reference area S as: on the abscissa the reference plant X b When the biomass percentage of (2) is 0%, the point of the corresponding salt concentration, the point of the biomass percentage of 100%, and the origin are taken as three endpoints of a rectangle, and a reference rectangle is drawn, wherein the area of the reference rectangle is S;
(4) Calculating the salt tolerance index T of each plant i ＝S i (iv)/S, wherein i is a positive integer selected from 1, 2 … … b, representing a different plant species or genotype, wherein b represents a reference plant.
2. The method of claim 1, wherein in step (2), the method of measuring biomass area is a function fitting method comprising fitting the plot to a function equation F (x) i ) And calculating the area of the area enclosed by the X-axis and the Y-axis by adopting a calculus method according to a function equation.
3. The method of claim 2, wherein the biomass linear equation F (x) resulting from the fitting i ) Degree of fitting R 2 ≥0.9。
4. The method as claimed in claim 1, wherein, in the step (2), the method for measuring biomass area is a direct measurement method, and the area of the region is directly measured by using an area measuring tool.
5. The method of claim 1, wherein, in step (3), the reference plant X b The plants with the strongest salt tolerance were evaluated.
6. The method of claim 5, wherein the reference plant X b The plant can tolerate the salt concentration of more than or equal to 600mM, and the salt concentration is the sodium salt concentration calculated by NaCl.
7. The method of claim 6, wherein the reference plant X b Is a halophyte;
preferably, said reference plant X b Is selected from Nitraria tangutorum, tamarix chinensis, suaeda salsa and Salicornia europaea.
8. The method according to claim 1, wherein in step (4) the salt tolerance index T of a plant is measured m And is combined with threshold index T n Comparing; then when T is m ≥T n When the plant has ideal salt tolerance, the plant is selected; when T is m ＜T n When the salt tolerance of the plant is insufficient, the plant is eliminated.
9. The method according to claim 1, wherein the method further comprises step (5): calculating relative salt tolerance index G of each plant i ＝T i /T b Wherein, T b ＝S b /S，S b Is the reference plant X b Biomass area of (1), G b ＝1。
10. The method of claim 9, wherein the salt tolerance index G of a plant is measured m And is combined with threshold index G n Comparing; when G is turned on m ≥G n When the plant has ideal salt tolerance, the plant is selected; when G is m ＜G n When the salt tolerance of the plant is insufficient, the plant is eliminated.
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