CN115657289A - Underwater microscope - Google Patents
Underwater microscope Download PDFInfo
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- CN115657289A CN115657289A CN202211587287.3A CN202211587287A CN115657289A CN 115657289 A CN115657289 A CN 115657289A CN 202211587287 A CN202211587287 A CN 202211587287A CN 115657289 A CN115657289 A CN 115657289A
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Abstract
The invention relates to the technical field of microscopes, in particular to an underwater microscope. The system comprises a bright field microscopic imaging device, a fluorescence microscopic imaging device and a control module, wherein the control module is respectively electrically connected with the bright field microscopic imaging device and the fluorescence microscopic imaging device, and the bright field microscopic imaging device and the fluorescence microscopic imaging device share the same microscopic imaging module. The underwater microscope solves the problems of narrow application range, poor imaging effect and inconvenient observation of a microscope with a single light source.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to an underwater microscope.
Background
The fluorescence microscope uses light with one or more specific wavelengths as a light source to irradiate an object to be detected and emit fluorescence, and then observes the shape and position of the object under the microscope. Fluorescence microscopy is used to study the absorption, transport, distribution and localization of chemical species within cells. Some substances in cells, such as chlorophyll and the like, can fluoresce after being irradiated by short-wave-band excitation light, and a fluorescence microscope is one of tools for carrying out qualitative and quantitative research on the substances.
However, the bright field microscope is a common microscopic method, and uses light illumination to image each point in the specimen in a bright background according to the difference of light absorption, and is widely used for observing specimens such as cells and tissue slices which are stained or have colors.
However, the related research and applications are mainly focused on land, and relatively few underwater research and applications. With the continuous and deep research on oceans by human beings, people begin to pay attention to the activities of micron-scale biological activities occurring in oceanic ecology such as seaweeds, seaweed beds, coral reefs and the like so as to research the influence of the activities on the health condition and the long-term dynamic evolution process of the oceanic ecology, such as coral albinism, coral worm growth, substrate structures and the like, so that the microscopic imaging technology has wide application requirements under water.
In the prior art, there is an underwater fluorescence microscope, as shown in fig. 1, including a left barrel 103, a right barrel 112, a middle connecting barrel 113, a cover plate 102, a waterproof plug 101, a handle, a laser module and an imaging module; the laser module comprises a laser light source 104 and a driver 105, and is arranged in the left barrel 103; the imaging module comprises a camera 111, a phase forming objective lens 110 and a filter 109, and is arranged in a right barrel 112; the cover plate 102 is arranged at the left end of the left barrel 103, and the cover plate 102 and the inner side of the left barrel 103 are waterproof through a sealing ring; the waterproof plug 101 is mounted on the cover plate 102, and the waterproof plug 101 is electrically connected with the driver 105; the middle part of the intermediate receiving tube 113 is hollowed, and the hollowed part is a sample space 107 which is an imaging range of the phase forming objective lens 110; transparent waterproof glass windows 106 and 108 are arranged at the two ends of the center of the intermediate connecting cylinder 113, which are close to the sample space 107, and light rays can penetrate through the transparent waterproof glass windows; the left barrel 103 and the right barrel 112 are connected and communicated through a middle connecting barrel 113, the inner side of the left barrel is waterproof through a sealing ring, and the whole cavity is kept sealed. However, this solution is a double-chamber design, and the object to be observed must be located in the sample space 107 between the two chambers, which is not convenient for observation without affecting marine benthos such as coral. In addition, only a fluorescent light source exists in the technical scheme, so that the application range is limited.
Disclosure of Invention
The invention provides an underwater microscope, aiming at solving the problems of narrow application range, poor imaging effect and inconvenient observation of a microscope with a single light source.
In a first aspect, the present invention provides an underwater microscope, including a bright field microscopic imaging device, a fluorescence microscopic imaging device, and a control module, where the control module is electrically connected to the bright field microscopic imaging device and the fluorescence microscopic imaging device, respectively, and the bright field microscopic imaging device and the fluorescence microscopic imaging device share the same microscopic imaging module.
The multifunctional cabin is characterized by further comprising a cabin body, wherein a front end cover and a rear end cover are respectively arranged at two ends of the cabin body; the front end cover and the rear end cover are respectively and fixedly connected with the cabin body in a sealing way to form a closed chamber together; the rear end cover is provided with a submarine cable joint, and the submarine cable joint is fixedly connected with the rear end cover in a sealing way; a supporting structure is fixedly arranged in the closed cavity, and the bright field microscopic imaging device, the fluorescence microscopic imaging device and the control module are fixedly arranged on the supporting structure.
In some embodiments, the microscopy imaging module comprises: the device comprises a microscope objective, a dichroic filter unit, a sleeve lens, a liquid zoom lens, a filter unit and an industrial camera which are sequentially arranged from front to back.
In some embodiments, the bright field microscopic imaging device further comprises a white light LED.
In some embodiments, the fluorescence microscopic imaging device further includes a fluorescence light source, a collimating lens group, and a mirror unit, which are sequentially arranged from back to front, the mirror unit can reflect fluorescence emitted from the fluorescence light source to a dichroic filter unit in the microscopic imaging module, and the dichroic filter unit enables the fluorescence entering the dichroic filter unit to irradiate on an object to be observed through a microscopic objective lens.
In some embodiments, the control module comprises an embedded controller, a lens controller, a voltage reduction module and a constant current module, wherein a submarine cable connector is electrically connected with an input end of the voltage reduction module, and an output end of the voltage reduction module supplies power to the whole underwater microscope; the embedded controller is respectively in electrical signal connection with the lens controller, the industrial camera, the signal input end of the constant current module and the submarine cable joint, and the signal output end of the constant current module is respectively in electrical signal connection with the white light LED and the fluorescent light source; and the signal output end of the lens controller is electrically connected with the liquid zoom lens.
In some embodiments, the battery further comprises a battery fixedly arranged on the support structure, and an input end of the battery is electrically connected with an output end of the voltage reduction module.
In some embodiments, the support structure comprises: the device comprises a front supporting plate, a rear supporting plate, an upper supporting plate and a lower supporting plate;
the front supporting plate is arranged at the end part close to the front end cover in the cabin body, the outer contour of the front supporting plate is matched with the inner shape of the cabin body, and the middle part of the front supporting plate is provided with a first through hole and a second through hole; the rear supporting plate is arranged at the end part close to the rear end cover in the cabin body, the outer contour of the rear supporting plate is matched with the inner shape of the cabin body, and the middle part of the rear supporting plate is provided with a third through hole; go up the backup pad with the bottom suspension fagging respectively horizontal set up in preceding backup pad with between the back backup pad, go up the backup pad with the both ends of bottom suspension fagging respectively with preceding backup pad with back backup pad fixed connection.
In some embodiments, the bright field microscopic imaging device and the fluorescence microscopic imaging device are both fixedly arranged on the upper surface of the lower support plate; the control module is fixedly arranged on the lower surface of the lower supporting plate; the submarine cable joint penetrates through the third through hole; and a microscope objective in the microscope imaging module passes through the first through hole.
In some embodiments, a plurality of first positioning rods, one end of which is fixed to the dichroic filter unit, are arranged around the microscope objective lens and fix the microscope objective lens from the circumferential direction; after the first positioning rod passes through the third through hole, the other end of the first positioning rod is fixedly connected with a white light LED in the bright field microscopic imaging device; the white light LED is annular, and the microscope objective penetrates through a central through hole of the white light LED.
Still further, the white light LED may be manually or automatically adjusted in brightness by an embedded controller.
Furthermore, when the LED is used as a fluorescent light source, manual or automatic brightness adjustment can be realized through the embedded controller; when a laser diode is used as the fluorescent light source, laser pulses can be emitted while the camera exposure is synchronously controlled.
The invention has the following advantages:
the technical scheme of the invention improves the light path structure of the underwater fluorescence microscope, realizes single-cabin design, only needs to align the microscope objective to the object to be observed, does not need to place the object to be observed between the light source and the imaging module, ensures that the observation is more convenient and easier to operate, and does not damage the shot object or surrounding organisms or ecological environment.
According to the technical scheme, the liquid zoom lens is added, the imaging depth of field of the microscope is enlarged, the system practicability is improved, and meanwhile, the liquid zoom lens can be used for scanning an imaging area to obtain a three-dimensional structure of a shot object in the depth direction.
The underwater microscope provided by the invention is provided with the bright field microscopic imaging device and the fluorescence microscopic imaging device at the same time, and combines the bright field microscopic imaging light path and the fluorescence microscopic imaging light path into a whole, so that the application range of the system is enlarged, and one device can meet various experimental requirements.
Drawings
FIG. 1 shows a block diagram of a prior art underwater fluorescence microscope;
FIG. 2 shows an overall external perspective view of an embodiment of the present invention;
FIG. 3 shows a perspective view of an embodiment of the invention with the outer walls of the nacelle and the front and rear end caps removed;
FIG. 4 shows a top view of the embodiment of FIG. 3 with the upper support plate and battery removed;
FIG. 5 shows a bottom view of the embodiment of FIG. 3;
FIG. 6 shows an optical path diagram of a fluorescence microscopy imaging device according to the invention;
FIG. 7 shows an optical path diagram of a bright field microscopic imaging apparatus according to the present invention;
fig. 8 shows the working flow of the underwater microscope of the present invention.
Detailed Description
The disclosure will now be discussed with reference to several exemplary embodiments. It should be understood that these embodiments are discussed only to enable those of ordinary skill in the art to better understand and thus implement the present disclosure, and are not intended to imply any limitation on the scope of the present disclosure.
As used herein, the term "include" and its variants are to be read as open-ended terms meaning "including, but not limited to. The term "based on" is to be read as "based, at least in part, on". The terms "one embodiment" and "an embodiment" are to be read as "at least one embodiment". The term "another embodiment" is to be read as "at least one other embodiment".
Referring to fig. 2, the present embodiment discloses an underwater microscope, which is a sealed cylinder and includes a cylindrical chamber 3, and a front end cap 2 and a rear end cap 4 are respectively disposed at two ends of the chamber 3; the front end cover 2 and the rear end cover 4 are respectively and fixedly connected with the cabin body 3 in a sealing way to form a closed chamber together; a submarine cable joint 5 is arranged at the rear end cover 4, and the submarine cable joint 5 is fixedly connected with the rear end cover 4 in a sealing way; a supporting structure is fixedly arranged in the closed cavity, and the bright field microscopic imaging device, the fluorescence microscopic imaging device and the control module are fixedly arranged on the supporting structure.
An optical window 1 is arranged at the front end cover 2, and light can penetrate through the optical window. The optical window 1 is preferably made of sapphire glass, and the chamber 3 and the front and rear end caps may be made of aluminum alloy, and may be sealed by sealing rings.
Referring to FIG. 3, a perspective view of an embodiment of the present invention is shown with the outer walls of the nacelle and the front and rear end caps removed. The supporting device comprises: the front support plate 403, the rear support plate 406, the upper support plate 405 and the lower support plate 407, the front support plate 403 is arranged at the end part of the cabin body 3 close to the front end cover 2, the external contour of the front support plate is adapted to the internal shape of the cabin body 3, and the middle part of the front support plate is provided with a first through hole and a second through hole; the rear supporting plate 406 is arranged at the end part of the cabin body 3 close to the rear end cover 4, the external contour of the rear supporting plate is matched with the internal shape of the cabin body, and the middle part of the rear supporting plate is provided with a third through hole; the upper supporting plate 405 and the lower supporting plate 407 are respectively horizontally arranged between the front supporting plate 403 and the rear supporting plate 406, and two ends of the upper supporting plate 405 and the lower supporting plate 407 are respectively fixedly connected with the front supporting plate 403 and the rear supporting plate 406.
In this embodiment, the front support plate 403 and the rear support plate 406 are both circular plates, the upper support plate 405 and the lower support plate 407 are both flat plates with two ends folded, and two ends of the upper support plate and the lower support plate are respectively fixedly connected with the front support plate and the rear support plate in a manner of gluing, or fixedly connected by bolts, or welding, or any one of conventional fixed connection manners such as snap connection.
The battery 404 is fixedly disposed on the upper surface of the upper support plate 405. Two lugs are respectively arranged at two ends of the battery 404, a threaded hole is arranged on each lug, and the battery 404 is fastened on the upper supporting plate 405 through screws. Of course, the battery 404 may be secured to the upper support plate in other ways. The voltage line of the submarine cable joint 5 is connected with the battery 404, and the output end of the battery 404 is respectively connected with each electronic component of the whole device. The battery 404 is a rechargeable battery that can be recharged when connected to an external power source and can power the entire device when not connected to an external power source.
Referring to fig. 4, a top view of the embodiment of fig. 3 is shown with the upper support plate and battery removed. On the lower support plate 407, a plurality of cage fixing plates 412 are provided by screw fixation, and these cage fixing plates 412 are divided into two groups, which are respectively arranged in series and arranged in parallel with each other. In the first cage fixing plate 412, a screw adapter plate 413 is fixedly arranged on one side, close to the rear support plate 406, of the cage fixing plate 412 closest to the rear support plate 406, and the industrial camera 414 is fixedly arranged on the cage fixing plate 412 through the screw adapter plate 413.
A filtering unit 411 is fixedly disposed in the central through hole of the first cage-type fixing plate 412. The filter unit 411 has one end optically connected to the industrial camera 414 and the other end optically connected to the liquid zoom lens 410. The other end of the liquid zoom lens 410 is connected to a sleeve lens 409, and the sleeve lens 409 is screwed to the second port of the dichroic filter unit 408, and is optically connected to the second port. The dichroic filter unit 408 is fastened to the lower support plate 407 by screws. A microscope objective 401 is fixedly screwed to a first port of the dichroic filter unit 408 opposite to the second port. A plurality of first assembling struts 402 are uniformly distributed around the microscope objective 401 at the first port of the dichroic filter unit 408, and the first assembling struts 402 and the microscope objective 401 penetrate through the first through hole of the front support plate 403 and extend outwards from the first through hole.
The ends of these first mounting struts 402 are provided with white LEDs. In this embodiment, the white LED400 is an annular structure, and the LED beads are disposed on the end surface of the annular structure facing the front end cover 2. Of course, the white LED400 may be configured in other shapes or configurations as desired. The front end face of the microscope objective 401 slightly protrudes from the front end face of the white LED 400.
The white LED400 may be manually or automatically adjusted in brightness via the embedded controller 422.
The second set of cage fixing plates 412 are disposed in series, and four second assembling poles 417 are respectively passed through holes of corresponding corners of four corners of each of the second set of cage fixing plates 412 and are protruded to both ends. At the end of the four second mounting struts 417 adjacent the rear support plate 406, a fluorescent light source 419 is fixedly disposed. At the central through hole of the second set of cage fixing plates 412, a collimating lens group 418 is arranged along the axis. A mirror unit 416 is fixedly disposed at the other end of the four second mounting struts 417, and the other side of the mirror unit 416 is connected to the third port of the dichroic filter unit 408 through a third mounting strut 415.
When the LED is used as a fluorescent light source, manual or automatic brightness adjustment can be realized through the embedded controller 422, and when the laser diode is used as the fluorescent light source, laser pulses can be emitted, and meanwhile, exposure of the camera is synchronously controlled, so that the signal-to-noise ratio is improved.
Referring to fig. 5, a bottom view of the embodiment of fig. 3 is shown. An embedded controller 422, a lens controller 423, a voltage reduction module 421 and a constant current module 420 are fixedly arranged on the lower surface of the lower support plate 407, a submarine cable connector 5 is electrically connected with the input end of the voltage reduction module 421, and the output end of the voltage reduction module 421 supplies power to the whole underwater microscope; the embedded controller 422 is respectively in electrical signal connection with the lens controller 423, the industrial camera 414, the signal input end of the constant current module 420 and the submarine cable joint 5, and the signal output end of the constant current module 420 is respectively in electrical signal connection with the white light LED400 and the fluorescent light source 419; the signal output terminal of the lens controller 423 is electrically connected to the liquid zoom lens 410.
The sea cable connector 5 can provide power for the whole microscope and also can carry out data transmission between the microscope and a remote control end. That is, the control command of the remote controller may be sent to the embedded controller 422, or the data obtained by the industrial camera 414 may be transmitted to the remote controller.
Referring to fig. 6, there is shown an optical path diagram of a fluorescence microscopy imaging device of the present invention. When fluorescence microscopic imaging is required, the fluorescence light source 419 emits light with a specific wavelength according to a control signal, the light with the specific wavelength is emitted into the reflector unit 416 through the collimating lens group 418, is reflected by the reflector unit 416, enters the dichroic filter unit 408, and then is emitted to an object to be observed through the microscope objective 401. The object to be observed emits fluorescence after being irradiated by the light with the specific wavelength, and the fluorescence sequentially passes through a microscope objective 401, a dichroic filter unit 408, a sleeve lens 409, a liquid zoom lens 410 and a filter unit 411, and finally enters an industrial camera 414.
Referring to fig. 7, there is shown an optical path diagram of a bright field microscopic imaging apparatus of the present invention. When bright field microscopic imaging is required, light emitted by the white light LED is irradiated on an object to be observed. The reflected light passes through a microscope objective 401, a dichroic filter unit 408, a sleeve lens 409, a liquid zoom lens 410, a filter unit 411 in sequence, and finally enters an industrial camera 414.
Referring to fig. 8, the workflow of the underwater microscope of the present invention is shown.
After the power is switched on, firstly, the working mode needs to be set: the first working mode is a manual acquisition mode, and the second working mode is an automatic acquisition mode; the third working mode is a timing acquisition mode.
In a first mode of operation, the operator can manually select a fluorescence photomicrograph, a bright field photomicrograph, or a combined bright field-fluorescence photomicrograph. The microscope can be controlled by manually inputting control signals through a remote control system, and can also be controlled through a switch arranged outside the microscope cabin. The switch can be respectively and electrically connected with the white light LED, the fluorescent light source, the liquid zoom lens and the industrial camera.
In a second working mode, the controller controls the microscope to shoot a fluorescence microscopic imaging picture as a sample, obtains the color gradation distribution condition of the picture through data analysis of the sample, and judges the result: if more sampling points with higher brightness exist, fluorescence microscopic shooting is adopted; if a small number of sampling points with higher brightness exist, bright field-fluorescence combined microscopic shooting is adopted; if the sampling points with higher brightness exist in the part, bright field microscopic shooting is adopted.
In the third operation mode, the time interval and the light source type can be input in advance, the microscope can shoot by adopting the preset shooting light source according to the preset time,
in the three modes, after the type of the light source is determined, the zoom lens can be controlled to change the focal length, an image stack scanned in the depth direction is obtained, and finally the images are fused and superposed to obtain the desired data.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples of the present disclosure and that various changes in form and details may be made therein without departing from the spirit and scope of the present disclosure in practice.
Claims (11)
1. An underwater microscope, characterized by: the system comprises a bright field microscopic imaging device, a fluorescence microscopic imaging device and a control module, wherein the control module is respectively electrically connected with the bright field microscopic imaging device and the fluorescence microscopic imaging device, and the bright field microscopic imaging device and the fluorescence microscopic imaging device share the same microscopic imaging module; the multifunctional cabin is characterized by further comprising a cabin body, wherein a front end cover and a rear end cover are respectively arranged at two ends of the cabin body; the front end cover and the rear end cover are respectively and fixedly connected with the cabin body in a sealing way to form a closed chamber together; the rear end cover is provided with a submarine cable joint, and the submarine cable joint is fixedly connected with the rear end cover in a sealing way; a supporting structure is fixedly arranged in the closed cavity, and the bright field microscopic imaging device, the fluorescence microscopic imaging device and the control module are fixedly arranged on the supporting structure.
2. An underwater microscope as claimed in claim 1, wherein the microscopic imaging module comprises: the device comprises a microscope objective, a dichroic filter unit, a sleeve lens, a liquid zoom lens, a filter unit and an industrial camera which are sequentially arranged from front to back.
3. An underwater microscope as in claim 1 wherein the bright field microscopic imaging device further comprises a white light LED.
4. An underwater microscope as claimed in any one of claims 1 to 3, wherein the fluorescence microscopic imaging apparatus further comprises a fluorescence light source, a collimating lens group and a mirror unit arranged from back to front, the mirror unit being capable of reflecting fluorescence emitted from the fluorescence light source into a dichroic filter unit in the microscopic imaging module, the dichroic filter unit causing the fluorescence entering therein to be irradiated onto an object to be observed through a microscope objective.
5. The underwater microscope of claim 1, wherein the control module comprises an embedded controller, a lens controller, a voltage reduction module and a constant current module, a submarine cable connector is electrically connected with an input end of the voltage reduction module, and an output end of the voltage reduction module supplies power to the whole underwater microscope; the embedded controller is respectively in electrical signal connection with the lens controller, the industrial camera, the signal input end of the constant current module and the submarine cable joint, and the signal output end of the constant current module is respectively in electrical signal connection with the white light LED and the fluorescent light source; and the signal output end of the lens controller is electrically connected with the liquid zoom lens.
6. An underwater microscope according to claim 1,
the battery is fixedly arranged on the supporting structure, and the input end of the battery is electrically connected with the output end of the voltage reduction module.
7. An underwater microscope as claimed in claim 1, wherein:
the support structure includes: the device comprises a front supporting plate, a rear supporting plate, an upper supporting plate and a lower supporting plate;
the front supporting plate is arranged at the end part close to the front end cover in the cabin body, the outer contour of the front supporting plate is matched with the inner shape of the cabin body, and the middle part of the front supporting plate is provided with a first through hole and a second through hole; the rear supporting plate is arranged at the end part close to the rear end cover in the cabin body, the outer contour of the rear supporting plate is matched with the inner shape of the cabin body, and the middle part of the rear supporting plate is provided with a third through hole; go up the backup pad with the bottom suspension fagging respectively horizontal set up in preceding backup pad with between the back backup pad, go up the backup pad with the both ends of bottom suspension fagging respectively with preceding backup pad with back backup pad fixed connection.
8. An underwater microscope as claimed in claim 7, wherein:
the bright field microscopic imaging device and the fluorescence microscopic imaging device are both fixedly arranged on the upper surface of the lower supporting plate; the control module is fixedly arranged on the lower surface of the lower supporting plate; the submarine cable joint penetrates through the third through hole; and a microscope objective in the microscope imaging module passes through the first through hole.
9. An underwater microscope as claimed in claim 7, wherein: a plurality of first positioning rods with one ends fixed on the dichroic filter unit are arranged around the microscope objective in a surrounding way and fix the microscope objective from the circumferential direction; after the first positioning rod passes through the third through hole, the other end of the first positioning rod is fixedly connected with a white light LED in the bright field microscopic imaging device; the white light LED is annular, and the microscope objective penetrates through a central through hole of the white light LED.
10. An underwater microscope as claimed in claim 3, wherein: the white light LED can realize manual or automatic brightness adjustment through an embedded controller.
11. An underwater microscope as claimed in claim 4, wherein: when the LED is used as a fluorescent light source, manual or automatic brightness adjustment can be realized through the embedded controller; when a laser diode is used as the fluorescent light source, laser pulses can be emitted while the camera exposure is synchronously controlled.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211587287.3A CN115657289A (en) | 2022-12-12 | 2022-12-12 | Underwater microscope |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211587287.3A CN115657289A (en) | 2022-12-12 | 2022-12-12 | Underwater microscope |
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| CN115657289A true CN115657289A (en) | 2023-01-31 |
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| CN101324702A (en) * | 2007-06-12 | 2008-12-17 | 卡尔蔡司微成像有限责任公司 | Microscope for observing a sample in the bright field illumination by transmitted light or in fluorescence-contrast epi-illumination |
| CN102518959A (en) * | 2011-11-18 | 2012-06-27 | 厦门大学 | Optically annular lighting device |
| US20190287760A1 (en) * | 2016-10-11 | 2019-09-19 | Focus-Ebeam Technology (Beijing) Co., Ltd. | Charged particle beam system, opto-electro simultaneous detection system and method |
| CN212255074U (en) * | 2020-04-24 | 2020-12-29 | 上海烈冰生物医药科技有限公司 | Multi-channel fluorescence full-field scanning imaging device |
| CN115355989A (en) * | 2022-08-25 | 2022-11-18 | 杭州蓝科光电科技有限公司 | Underwater multispectral microscope capable of automatically adjusting wavelength |
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2022
- 2022-12-12 CN CN202211587287.3A patent/CN115657289A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101324702A (en) * | 2007-06-12 | 2008-12-17 | 卡尔蔡司微成像有限责任公司 | Microscope for observing a sample in the bright field illumination by transmitted light or in fluorescence-contrast epi-illumination |
| CN101216601A (en) * | 2007-12-29 | 2008-07-09 | 中国科学院西安光学精密机械研究所 | Method and device for accomplishing dark-field photomicrography and fluorescent photomicrography by axicon lens |
| CN102518959A (en) * | 2011-11-18 | 2012-06-27 | 厦门大学 | Optically annular lighting device |
| US20190287760A1 (en) * | 2016-10-11 | 2019-09-19 | Focus-Ebeam Technology (Beijing) Co., Ltd. | Charged particle beam system, opto-electro simultaneous detection system and method |
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| CN115355989A (en) * | 2022-08-25 | 2022-11-18 | 杭州蓝科光电科技有限公司 | Underwater multispectral microscope capable of automatically adjusting wavelength |
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