CN115650872B - Separation and purification method of L-homoserine fermentation liquor - Google Patents
Separation and purification method of L-homoserine fermentation liquor Download PDFInfo
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- UKAUYVFTDYCKQA-VKHMYHEASA-N L-homoserine Chemical compound OC(=O)[C@@H](N)CCO UKAUYVFTDYCKQA-VKHMYHEASA-N 0.000 title claims abstract description 133
- UKAUYVFTDYCKQA-UHFFFAOYSA-N -2-Amino-4-hydroxybutanoic acid Natural products OC(=O)C(N)CCO UKAUYVFTDYCKQA-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 238000000855 fermentation Methods 0.000 title claims abstract description 63
- 230000004151 fermentation Effects 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000000746 purification Methods 0.000 title abstract description 9
- 238000000926 separation method Methods 0.000 title abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 47
- 238000001728 nano-filtration Methods 0.000 claims abstract description 29
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 239000000919 ceramic Substances 0.000 claims abstract description 14
- 238000001471 micro-filtration Methods 0.000 claims abstract description 14
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- 239000002253 acid Substances 0.000 claims abstract description 6
- 238000002425 crystallisation Methods 0.000 claims abstract description 6
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- 239000007788 liquid Substances 0.000 claims description 25
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
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- 238000001816 cooling Methods 0.000 claims description 7
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- 150000003384 small molecules Chemical class 0.000 claims description 6
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- 238000000643 oven drying Methods 0.000 claims description 2
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- 238000005516 engineering process Methods 0.000 abstract description 9
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- 239000003513 alkali Substances 0.000 abstract description 2
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- LOTKRQAVGJMPNV-UHFFFAOYSA-N 1-fluoro-2,4-dinitrobenzene Chemical compound [O-][N+](=O)C1=CC=C(F)C([N+]([O-])=O)=C1 LOTKRQAVGJMPNV-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
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- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- INQOMBQAUSQDDS-UHFFFAOYSA-N iodomethane Chemical compound IC INQOMBQAUSQDDS-UHFFFAOYSA-N 0.000 description 2
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- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
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- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
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- FZFAMSAMCHXGEF-UHFFFAOYSA-N chloro formate Chemical compound ClOC=O FZFAMSAMCHXGEF-UHFFFAOYSA-N 0.000 description 1
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 description 1
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- 229940107700 pyruvic acid Drugs 0.000 description 1
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Abstract
The invention discloses a separation and purification method of L-homoserine fermentation liquor. The invention relates to the field of separation and purification of L-homoserine, in particular to a method for separating and purifying L-homoserine from fermentation liquor, which comprises the following process steps: sterilizing the fermentation liquor, filtering with ceramic microfiltration membrane to remove impurities and bacteria, then introducing into organic nanofiltration membrane to remove sugar and partial pigment, decolorizing with active carbon, concentrating under reduced pressure, adding organic solvent for crystallization, and filtering to obtain L-homoserine. The invention adopts the organic nanofiltration membrane technology to remove sugar and pigment from fermentation liquor for the first time, and compared with the ion exchange technology, the invention reduces the use of a large amount of acid and alkali and the generation of wastewater; compared with electrodialysis technology, the method has the advantages of low equipment investment and low operation energy consumption. Most importantly, the removal of sugar effectively reduces the Maillard effect between sugar and amino acid, so that the final product has good appearance and high purity.
Description
Technical Field
The invention belongs to the technical field of separation and purification of amino acid, and particularly relates to a separation and purification method of L-homoserine fermentation liquor.
Background
L-homoserine (S-2-amino-4-hydroxybutyric acid) and derivatives thereof are used as chiral intermediates, have better application potential in the field of chiral chemical synthesis, for example, the L-homoserine has the same molecular skeleton as L-glufosinate, and are ideal chiral precursors for preparing the L-glufosinate. The glufosinate belongs to phosphonic acid herbicides, has the characteristics of wide herbicidal spectrum, low toxicity, high activity, good environmental compatibility and the like, and has slower acting speed than paraquat and better acting speed than glyphosate. Along with the guidance of the policy of gradually reducing the use of the glyphosate by national regulations, the glufosinate has wide market prospect. In addition, L-homoserine is a naturally occurring non-protein amino acid, has a basic skeleton of L-type-alpha-amino acid, can be converted into other important chemical intermediates through enzymatic reaction, and has rich biological activity, such as effective inhibition of sickle cells and antifungal. Therefore, the L-homoserine has important application prospect in the aspects of pharmacology, physiology and the like.
Currently, the industrial methods for producing L-homoserine mainly include chemical synthesis and biological methods. The chemical synthesis method is mainly a methionine method, salt is formed by reaction with methyl iodide, then water is added for dissolution and reflux, L-homoserine is generated under alkaline conditions, the method has high yield, but methyl iodide has high price, long reaction time and complex waste treatment; the biological method mainly comprises the steps of separating and purifying L-homoserine from fermentation liquor, wherein the L-homoserine fermentation liquor is a mixture with complex components, and besides the L-homoserine, a large amount of components such as thalli, proteins, residual sugar, pigments, inorganic salts and the like exist, and the removal of the proteins, the pigments, the residual sugar and the inorganic salts is the key for extracting the L-homoserine.
The patent CN105506014A provides a method for preparing L-homoserine by using pyruvic acid and aldehyde compounds as substrates and carrying out catalytic reaction under the action of aldolase, wherein the obtained product sequentially undergoes the steps of chromatographic separation, desalination, decoloration, concentration crystallization, drying and the like, and the L-homoserine is prepared by adopting ion exchange resin as a key extraction step in the extraction process.
Patent CN112694413a discloses a method for extracting L-homoserine from fermentation broth, comprising steps of pretreatment of fermentation broth, decolorization, electrodeionization, concentration filtration and the like, wherein: and (3) flocculating and settling the fermentation liquor, treating the fermentation liquor by a ceramic microfiltration membrane and an ultrafiltration membrane, decolorizing the fermentation liquor by an active carbon fiber module device, desalting the fermentation liquor by an electrodeionization device, concentrating the fermentation liquor, and adding an organic solvent for crystallization. The technology has long process path, more devices and low product yield.
Patent CN114989026A discloses a refining and purifying method of L-homoserine fermentation liquor, which comprises the steps of firstly adding carbonate and/or bicarbonate into the fermentation liquor, mixing, then dropwise adding chloroformate, preserving heat after the dropwise adding, evaporating water, extracting to obtain an intermediate; and mixing the obtained intermediate with hydrochloric acid solution, heating for reaction, steaming out the mixture of hydrochloric acid and water after the reaction is finished, adding propylene oxide for reaction, steaming out after the reaction is finished, adding alcohol solvent, stirring, separating out solid, and suction-filtering to obtain L-homoserine white solid. In the technology, a large amount of chemical reagent and concentrated hydrochloric acid are required to be added, and high-temperature reflux is required in the process.
In view of the above, a new method for separating and purifying L-homoserine fermentation broth is urgently needed.
Disclosure of Invention
The invention provides a separation and purification method of L-homoserine fermentation liquor aiming at the problems. The method for extracting L-homoserine has high final yield and high purity.
The aim and the technical problems of the invention are realized by adopting the following technical proposal.
The invention provides a separation and purification method of L-homoserine fermentation liquor, which is characterized by comprising the following steps:
S1: sterilizing, namely sterilizing fermentation liquor;
S2: sterilizing, namely passing the sterilized fermentation liquor through a ceramic microfiltration membrane, and collecting clear liquor of the fermentation liquor;
s3: removing sugar, pigment and small molecules, passing the clear solution of the fermentation liquor through an organic nanofiltration membrane, and collecting clear solution of the nanofiltration membrane;
S4: decolorizing, namely adding acid into the collected nanofiltration membrane clear liquid to adjust the pH to be between 5 and 6, adding activated carbon for decolorizing, and collecting decolorized liquid;
s5: concentrating and filtering, concentrating the decolorized solution under reduced pressure until the L-homoserine content is 80-100%, adding organic solvent with 3-6 times of the volume of the concentrated solution under stirring, cooling to-5-0deg.C, maintaining the temperature for crystallization for a certain time, filtering, and oven drying to obtain L-homoserine.
Further, the concentration of L-homoserine in the fermentation broth in the step S1 is 100-200g/L.
Further, the sterilization conditions in step S1 are: the temperature is 40-120 ℃ and the time is 10-60min.
Further, the filtering conditions in step S2 are: the aperture of the ceramic microfiltration membrane is 0.01-0.2 mu m, and the operating pressure is 0.1-0.5 MPa.
Further, the filtering conditions in step S3 are: the molecular weight of the organic nanofiltration membrane is 100-1000 Da, and the operating pressure is 1.5-4.0 MPa.
Further, in the step S4, the acid is hydrochloric acid, and the concentration is 20-37%.
Further, in the step S4, the addition amount of the activated carbon is 0.1-2% V, and the decoloring temperature is 30-90 ℃.
Further, the transmittance of the decolorized solution in step S4 is 90-100%.
Further, in step S5, the organic solvent is at least one of methanol, ethanol, isopropanol, propanol, and acetone.
By means of the technical scheme, the invention has at least the following advantages: the invention adopts the organic nanofiltration membrane technology to remove sugar, partial pigment, small molecular impurities and the like from the L-homoserine fermentation liquor for the first time. First, compared with ultrafiltration technology, the method reduces the consumption of active carbon in the subsequent decolorization step, greatly reduces the generation of waste carbon, and simultaneously effectively improves the purity and color of the product. Secondly, compared with electrodialysis technology, the equipment investment is small, and the operation and maintenance cost is low; compared with the ion exchange resin technology, the method reduces the use of a large amount of acid and alkali and reduces the generation of wastewater. Thirdly, the removal of the residual sugar effectively avoids the Maillard effect generated between the residual sugar and the amino acid, and improves the quality and purity of the L-homoserine. The L-homoserine extracted from the fermentation liquor by the method has white color, high purity and high yield, and the final yield of the L-homoserine extracted by the method is more than 80% and the purity is more than 90%.
The foregoing description is only an overview of the present invention, and is intended to provide a more thorough understanding of the present invention, and is to be accorded the full scope of the present invention.
Drawings
FIG. 1 is a flow chart of the separation and purification process of the L-homoserine fermentation liquid of the invention.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement of the purposes and the effects of the present invention easy to understand, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention and the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
If not specified, the purity of the L-homoserine is determined by high performance liquid chromatography, and the specific detection method is as follows: agilent (Agilent-1200) high performance liquid chromatography was used to determine the components in the fermentation broth. The sample is diluted properly and then is derivatized by using 2, 4-Dinitrofluorobenzene (DNFB), 50 uL of 10 g/L DNFB acetonitrile solution and 100 uL 0.5M NaHCO 3 solution are added into 100 uL samples, the mixture is fully and uniformly mixed, the mixture is subjected to light-shielding reaction at 60 ℃ for 1h, 750 uL of 0.01M KH 2PO4 solution is added after cooling, the mixture is uniformly mixed, and the mixture is filtered by using a 0.22 um filter membrane and then is subjected to high performance liquid chromatography detection. The chromatographic column was ZORBAX Eclipse XDB-C18 column (4.6X106: 150 mm, 5 um; agilent), the column temperature was 30deg.C, the mobile phase was 35% acetonitrile formic acid (thousandth) aqueous solution, the flow rate was 1 mL/min, and the detection wavelength was 360 nm. The method is an external standard method, and the standard product is purchased from Shanghai Ala Biochemical technology Co., ltd, and the product number is H105430.
Example 1
A method for separating and purifying L-homoserine from fermentation broth, comprising the following steps:
S1: and (3) sterilization: sterilizing 100L fermentation broth (L-homoserine concentration of 100-200 g/L) at 40deg.C for 30min;
s2: and (3) sterilization: removing thalli and macromolecular proteins from the sterilized fermentation liquor by using a ceramic microfiltration membrane, and collecting 100L of fermentation liquor clear liquid, wherein the aperture of the ceramic microfiltration membrane is 0.01 mu m, and the operating pressure is 0.5MPa;
S3: removing sugar, pigment and small molecule: removing residual sugar, small molecular peptide, short chain protein and the like in the clear fermentation broth in the step S2 by using an organic nanofiltration membrane, and collecting 100L of the clear nanofiltration membrane, wherein the molecular weight of the organic nanofiltration membrane is 100 Da, and the operating pressure is 4.0 MPa;
s4: decoloring: adding hydrochloric acid (with the concentration of 20%) into the nanofiltration membrane clear liquid collected in the step S3 to adjust the pH value to 5.0, adding active carbon into the nanofiltration membrane clear liquid to decolor for 40min, and collecting decolored liquid, wherein the light transmittance of the decolored liquid is 98%, the addition amount of the active carbon is 0.1% V, and the decolored temperature is 60 ℃;
S5: concentrating and filtering: concentrating the decolorized solution obtained in the step S4 under reduced pressure until the L-homoserine content is 80%, adding absolute ethyl alcohol with the volume of 6 times of the concentrated solution into the decolorized solution under stirring, cooling to-5 ℃, preserving heat and crystallizing for 12 hours, filtering and drying to obtain the L-homoserine.
The L-homoserine was found to be 12.3kg, and the purity thereof was found to be 95% by HPLC analysis, and the yield was found to be 82%.
Example 2
A method for separating and purifying L-homoserine from fermentation broth, comprising the following steps:
S1: and (3) sterilization: sterilizing 100L fermentation broth (L-homoserine concentration of 100-200 g/L) at 60deg.C for 30min;
S2: and (3) sterilization: removing thalli and macromolecular proteins from the sterilized fermentation liquor by using a ceramic microfiltration membrane, and collecting 100L of fermentation liquor clear liquid, wherein the aperture of the ceramic microfiltration membrane is 0.03 mu m, and the operating pressure is 0.3MPa;
S3: removing sugar, pigment and small molecule: removing residual sugar, small molecular peptides, short chain proteins and the like in the clear fermentation broth in the step S2 by using an organic nanofiltration membrane, and collecting 100L of the clear nanofiltration membrane, wherein the molecular weight of the organic nanofiltration membrane is 500 Da, and the operating pressure is 2.5 MPa;
s4: decoloring: adding hydrochloric acid (with the concentration of 25%) into the nanofiltration membrane clear liquid collected in the step S3 to adjust the pH to 5.5, adding active carbon into the nanofiltration membrane clear liquid to decolor for 1h, collecting decolored liquid, wherein the light transmittance of the decolored liquid is 95%, the adding amount of the active carbon is 1%V, and the decoloration temperature is 70 ℃;
S5: concentrating and filtering: concentrating the decolorized solution obtained in the step S4 under reduced pressure until the L-homoserine content is 85%, adding isopropanol with the volume 5 times of the concentrated solution into the decolorized solution under stirring, cooling to 0 ℃, preserving heat and crystallizing for 15h, filtering and drying to obtain L-homoserine.
The L-homoserine was found to be 12.75kg, and the purity was detected by high performance liquid chromatography to be 93%, and the yield was calculated to be 85%.
Example 3
A method for separating and purifying L-homoserine from fermentation broth, comprising the following steps:
S1: and (3) sterilization: sterilizing 100L fermentation broth (L-homoserine concentration of 100-200 g/L) at 110deg.C for 10min;
S2: and (3) sterilization: removing thalli and macromolecular proteins from the sterilized fermentation liquor by using a ceramic microfiltration membrane, and collecting 100L of fermentation liquor clear liquid, wherein the aperture of the ceramic microfiltration membrane is 0.2 mu m, and the operating pressure is 0.1MPa;
S3: removing sugar, pigment and small molecule: removing residual sugar, small molecular peptide, short chain protein and the like in the clear fermentation broth in the step S2 by using an organic nanofiltration membrane, and collecting 100L of the clear nanofiltration membrane, wherein the molecular weight of the organic nanofiltration membrane is 1000 Da, and the operating pressure is 1.5 MPa;
S4: decoloring: adding hydrochloric acid (with the concentration of 30%) into the nanofiltration membrane clear liquid collected in the step S3 to adjust the pH to 6.0, adding active carbon into the nanofiltration membrane clear liquid to decolor for 2 hours, collecting decolored liquid, wherein the light transmittance of the decolored liquid is 92%, the adding amount of the active carbon is 1.5% V, and the decoloration temperature is 80 ℃;
S5: concentrating and filtering: concentrating the decolorized solution obtained in the step S4 under reduced pressure until the L-homoserine content is 90%, adding methanol with the volume 5 times of the concentrated solution into the decolorized solution under stirring, cooling to-5 ℃, preserving heat, crystallizing for 15h, filtering and drying to obtain L-homoserine.
The L-homoserine was found to be 13.05kg, and the purity thereof was found to be 90% by HPLC, and the yield thereof was found to be 87%.
Comparative example 1
The fermentation broth used in example 3 was extracted by ultrafiltration membrane filtration, and the specific steps were as follows:
S1: and (3) sterilization: sterilizing 100L fermentation broth (L-homoserine concentration of 100-200 g/L) at 110deg.C for 10min;
S2: and (3) sterilization: removing thalli and macromolecular proteins from the sterilized fermentation liquor by using a ceramic microfiltration membrane, and collecting 100L of fermentation liquor clear liquid, wherein the aperture of the ceramic microfiltration membrane is 0.2 mu m, and the operating pressure is 0.1MPa;
S3: removing sugar, pigment and small molecule: filtering the clear fermentation broth in the step S2 by using an organic ultrafiltration membrane, and collecting 100L of the clear ultrafiltration membrane, wherein the molecular weight of the organic ultrafiltration membrane is 10 kDa, and the operating pressure is 0.5 MPa;
S4: decoloring: adding hydrochloric acid (with the concentration of 30%) into the nanofiltration membrane clear liquid collected in the step S3 to adjust the pH to 6.0, adding active carbon into the nanofiltration membrane clear liquid to decolor for 2 hours, collecting decoloration liquid, wherein the light transmittance of the decoloration liquid is 75%, the addition amount of the active carbon is 1.5% V, and the decoloration temperature is 80 ℃;
S5: concentrating and filtering: concentrating the decolorized solution obtained in the step S4 under reduced pressure until the L-homoserine content is 90%, adding methanol with the volume 5 times of the concentrated solution into the decolorized solution under stirring, cooling to-5 ℃, preserving heat for crystallization 15 h, filtering and drying to obtain the tan L-homoserine.
The L-homoserine was found to be 17.55 kg, and its purity was found to be 50% by liquid chromatography, with a yield of 65%.
As can be seen from the comparison of examples 1-3 and comparative examples, the transmittance of the decolorized solution is 92% and 75% respectively under the same activated carbon decolorization conditions, which indicates that the examples are significantly better than the comparative examples in decolorizing effect under the same activated carbon addition and decolorization conditions; in addition, as can be seen from the comparison of the yields and purities of the two final products, the examples are significantly superior to the comparative examples in terms of product appearance, yield and purity, so that the nanofiltration method is more suitable for extracting L-homoserine.
While the invention has been described with respect to preferred embodiments, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention, and that any such changes and modifications as described in the above embodiments are intended to be within the scope of the invention.
Claims (8)
1. A method for separating and purifying an L-homoserine fermentation broth, which is characterized by comprising the following steps:
S1: sterilizing, namely sterilizing fermentation liquor;
S2: sterilizing, namely passing the sterilized fermentation liquor through a ceramic microfiltration membrane, and collecting clear liquor of the fermentation liquor;
s3: removing sugar, pigment and small molecules, passing the clear solution of the fermentation liquor through an organic nanofiltration membrane, and collecting clear solution of the nanofiltration membrane;
S4: decolorizing, namely adding acid into the collected nanofiltration membrane clear liquid to adjust the pH to be between 5 and 6, adding activated carbon for decolorizing, and collecting decolorized liquid;
S5: concentrating and filtering, concentrating the decolorized solution under reduced pressure until the L-homoserine content is 80-100%, adding an organic solvent with the volume 3-6 times of the concentrated solution under stirring, cooling to-5-0deg.C, maintaining the temperature for crystallization for a certain time, filtering, and oven drying to obtain L-homoserine;
The filtering conditions in the step S3 are as follows: the molecular weight of the organic nanofiltration membrane is 100-1000 Da, and the operating pressure is 1.5-4.0 MPa.
2. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the concentration of L-homoserine in the fermentation broth in step S1 is 100-200g/L.
3. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the sterilization conditions in step S1 are as follows: the temperature is 40-120 ℃ and the time is 10-60min.
4. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the filtration conditions in step S2 are as follows: the aperture of the ceramic microfiltration membrane is 0.01-0.2 mu m, and the operating pressure is 0.1-0.5 MPa.
5. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the acid in step S4 is hydrochloric acid, and the concentration is 20-37%.
6. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the addition amount of the activated carbon in the step S4 is 0.1-2% V, and the decolorization temperature is 30-90 ℃.
7. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the transmittance of the decolorized solution in step S4 is 90-100%.
8. The method for separating and purifying L-homoserine fermentation broth according to claim 1, wherein the organic solvent in step S5 is at least one of methanol, ethanol, isopropanol, propanol, and acetone.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101812009A (en) * | 2010-04-28 | 2010-08-25 | 河南巨龙淀粉实业有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
| JP2017051117A (en) * | 2015-09-08 | 2017-03-16 | Jnc株式会社 | Separation and purification methods of fermentation products |
| CN108084041A (en) * | 2017-12-20 | 2018-05-29 | 大连医诺生物股份有限公司 | A kind of method of efficient process zymotic fluid production amino acid |
| CN112694413A (en) * | 2021-03-24 | 2021-04-23 | 鲁东大学 | Method for extracting L-homoserine from fermentation liquor |
| CN114989026A (en) * | 2022-06-23 | 2022-09-02 | 苏州润亚旭日生物科技有限公司 | Refining and purifying method of L-homoserine fermentation broth |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812009A (en) * | 2010-04-28 | 2010-08-25 | 河南巨龙淀粉实业有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
| JP2017051117A (en) * | 2015-09-08 | 2017-03-16 | Jnc株式会社 | Separation and purification methods of fermentation products |
| CN108084041A (en) * | 2017-12-20 | 2018-05-29 | 大连医诺生物股份有限公司 | A kind of method of efficient process zymotic fluid production amino acid |
| CN112694413A (en) * | 2021-03-24 | 2021-04-23 | 鲁东大学 | Method for extracting L-homoserine from fermentation liquor |
| CN114989026A (en) * | 2022-06-23 | 2022-09-02 | 苏州润亚旭日生物科技有限公司 | Refining and purifying method of L-homoserine fermentation broth |
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