CN115650792A - Preparation method of compound microbial fertilizer for preventing and treating southern rust of corn - Google Patents
Preparation method of compound microbial fertilizer for preventing and treating southern rust of corn Download PDFInfo
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- CN115650792A CN115650792A CN202211297605.2A CN202211297605A CN115650792A CN 115650792 A CN115650792 A CN 115650792A CN 202211297605 A CN202211297605 A CN 202211297605A CN 115650792 A CN115650792 A CN 115650792A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 73
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- 150000001875 compounds Chemical class 0.000 title claims abstract description 55
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 240000008042 Zea mays Species 0.000 title claims description 71
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims abstract description 60
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- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn, belonging to the technical field of compound fertilizers and comprising the following steps: step 1, weighing a microbial agent, potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive; and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving, adding a microbial agent, granulating, and drying, wherein the microbial agent is prepared by mixing paenibacillus polymyxa and trichoderma. The compound microbial fertilizer can meet the nutritional requirements of corns in the growth process, the yield per mu reaches more than 670 kg, and the infection of multiple piles of puccinia striiformis can be resisted to prevent and control southern rust.
Description
Technical Field
The invention relates to the technical field of compound microbial fertilizers, in particular to a preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn.
Background
Corn is the major food crop challenge in the world and occupies an important position in food production in China. In recent years, the occurrence and prevalence of southern rust have become one of the major factors affecting corn yield. The southern rust disease of corn is a fungal disease taking Puccinia polycarpa as a pathogen, which mainly damages leaves and can also infect leaf sheaths, fruit ears, bracts and male flowers, wherein the primary infected lesion spots are water stain-shaped and small green spots, and later gradually develop into round or long round, yellow brown or brown spot spots, namely summer spore piles. Both sides of the leaf can be generated, the front side of the leaf is more, the quantity is more, the distribution is dense, golden summer spores are scattered after the sporophyte cracks, and the leaf is covered with the sporophyte in severe cases, so that the plant withers. Southern rust causes serious loss once outbreak occurs, so that the development of a microbial compound fertilizer capable of preventing and controlling southern rust of corn is needed.
The patent document with the publication number of 108383560A discloses a crop microbial compound fertilizer, which is designed for corn growth and has the advantages of promoting corn growth and precocity, increasing yield, reducing disease occurrence, improving corn quality, improving the micro-ecological environment of soil, restoring soil fertility and the like. The corn microbial compound fertilizer comprises pseudomonas, streptomyces jingyangensis, nitrogen fixing bacteria and phosphorus dissolving bacteria.
Patent document No. 103936468 discloses a corn compound fertilizer, which comprises: urea, monocalcium phosphate, potassium chloride, magnesium sulfate, boric acid, ammonium molybdate, copper sulfate, silicon fertilizer, cottonseed hulls, EM (effective microorganisms), glucose, pig manure, chicken manure, compound amino acid and corn straws; the corn compound fertilizer disclosed by the invention uses organic components and EM (effective microorganisms) bacteria, so that the activity of the fertilizer is improved, and after the corn compound fertilizer is used, microorganisms can improve soil, the activity and the air permeability of the soil are increased, the absorption rate of corn on the fertilizer can be improved, and the salinization of the soil is prevented.
The two compound fertilizers can improve the yield of the corn to a certain extent, but cannot improve the antibacterial property and prevent the southern rust of the corn.
Disclosure of Invention
In view of the above, the invention provides a preparation method of a composite microbial fertilizer for preventing and treating southern rust of corn.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing a microbial agent, potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving, adding a microbial agent, mixing, granulating and drying.
Further, the microbial agent is formed by mixing paenibacillus polymyxa and trichoderma.
Further, in the microbial agent, the weight parts of the components are as follows: 1.5-2 parts of bacillus polymyxa and 1 part of trichoderma.
Furthermore, the total effective viable count in the microbial agent is more than 0.2 hundred million/gram.
Further, the weight parts of the components are as follows: 10-15 parts of microbial agent, 5-8 parts of potassium lignosulfonate, 12-15 parts of calcium nitrate, 15-18 parts of potassium nitrate, 15-20 parts of diammonium hydrogen phosphate and 12-17 parts of additive.
Further, the additive comprises bentonite, mushroom residue, zinc sulfate, ammonium molybdate and bone meal.
Further, in the additive, the weight parts of each component are as follows: 15-20 parts of bentonite, 8-10 parts of mushroom residue, 2-3 parts of zinc sulfate, 2-3 parts of ammonium molybdate and 20-25 parts of bone meal.
Further, the mushroom dregs are obtained by crushing and fermenting for 7 days.
Furthermore, rice hulls and peanut shells are added in the mushroom residue fermentation process.
Further, the bone meal is decocted bone stock powder.
Southern rust is an important leaf disease in the middle and later growth stages of corn, mainly occurs in the corn filling stage, can cause great yield reduction of corn in severe cases, needs to be prevented and controlled in time, and reduces the occurrence and danger of the southern rust. The major causes of southern rust are mainly the following: 1. sufficient bacteria sources exist, when stubbles are not boiled in time, a large amount of straws and disease residues are retained in the field, pathogenic bacteria are continuously accumulated and overwinter by using winter spores, and the pathogenic bacteria become sources of infection in the next year. 2. The change of the climate is obvious along with the global warming trend, and the survival and the transmission of spores are facilitated in the growing process of the corn in the weather of high temperature, high humidity, overcast and rainy days and the like. The rust puccinia polycarpa summer spores can be infected only under suitable conditions in a short time, and the temperature and the humidity are important restriction factors for germination and infection of the summer spores in the southern rust infection cycle of the corn. 3. Improper fertilization, such as excessive use of nitrogen fertilizer, can reduce corn resistance and aggravate southern rust.
At present, the method for preventing and controlling the southern rust of corn mainly comprises the following steps: selecting three types of disease-resistant quality, scientific field management and chemical prevention and control. Weak resistance in maize varieties is an important factor that contributes to the development of southern rust in maize. The plant has the capability of resisting plant diseases and insect pests, the disease-resistant variety is the most economic and effective method for preventing and treating the diseases and can be used for cultivating more disease-inducing resistant varieties, such as 108 great farmlands, 50 Ludan seeds and the like, and the disease-resistant varieties are selected according to self conditions in different areas. In general, early-maturing varieties, sweet varieties, she Pianhuang, and varieties with few leaf coats have poor disease resistance, and dent varieties have strong disease resistance. The field management mainly focuses on water and fertilizer management and field sanitation. The seeding time is properly adjusted according to the local climate, and the germ infection time is avoided. The method avoids the partial application of nitrogen fertilizer, reasonably applies nitrogen, phosphorus and potassium fertilizer and additionally applies zinc fertilizer to improve the disease resistance of plants. And scientifically managing the water. In drought seasons, a drip irrigation mode is adopted, and flood irrigation not only can cause waste of water resources, but also can cause water accumulation, so that the stress resistance of plants is reduced, and the plants are easy to be infected with diseases. When water is accumulated, water needs to be drained in time, and low-water-injection sowing is avoided. Before planting corn, the stems and leaves of the corn in the field and the diseased residues need to be removed in time, and the centralized treatment is carried out to reduce the field bacterial sources. Crop rotation with non-gramineous crops is carried out as much as possible, and the occurrence degree of corn diseases is reduced. Chemical control means that chemical agents are used for inhibiting and killing pathogenic bacteria or passivating toxic metabolites of pathogenic bacteria to achieve the purpose of controlling plant diseases, and is generally realized by measures such as agent spraying, agent seed dressing, seed coating and the like. In order to prevent and control the pathogenic bacteria of the seeds, the seeds can be coated before sowing, so that the pathogeny is eradicated from the source.
At present, those skilled in the art are keen to develop corn disease-resistant varieties and drugs for preventing southern rust. The patent document with the publication number CN109206493A discloses the application of Zm-Remorin gene in the control of southern rust of corn, and provides the application; 1) Protein Zm-Remorin; 2) A DNA molecule encoding the protein Zm-Remorin; 3) A recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing a DNA molecule for encoding the protein Zm-Remorin. According to the literature, through the identification and expression analysis of the disease resistance of the Zm-Remorin transgenic line, researches show that after rust disease inoculation, the number and the size of disease spots of the Zm-Remorin transgenic positive line are obviously smaller than those of the negative line, and by combining with real-time fluorescent quantitative PCR analysis, the Zm-Remorin can be stably expressed in the transgenic positive line in a large amount and is obviously higher than those of the negative line, and the fact that the expression of the Zm-Remorin in a corn plant can improve the resistance to southern rust disease can be found. For another example, liu Lifeng, xu Fasan, huang Yinghui, different agents are used for the research on the effect of preventing and treating southern rust [ J ], plant protection science, and the research results of the effect of preventing and treating southern rust of corn by using different medicinal vitex disclose that 20% Yang Cai suspoemulsion, 32.5% amimo suspension, 30% anoectochile missible oil and 25% amioda suspoemulsion have good effects on preventing and treating southern rust of corn, and reach 76.12% -90.96%. While chemical control can effectively control southern corn induced diseases, it can damage the surrounding environment and the viability of the root system Wang Rangwei biota. In addition, the ever-increasing cost of pesticides and consumer demand for pesticide-free foods have led to the search for alternatives, and moreover, long-term use of drugs is prone to developing resistance. Therefore, how to use no or less chemical pesticide in the prevention and control of corn diseases has important significance for protecting ecological environment and reducing agricultural non-point source pollution.
The invention has the beneficial effects that:
the potassium lignosulfonate is purchased granules of 80 meshes, the lignin content is 55-60%, and the granules contain organic matters, humic acid, nitrogen, potassium, phosphorus, sodium, calcium, magnesium, iron, zinc and other elements.
The microbial agent is prepared by mixing paenibacillus polymyxa and trichoderma. Paenibacillus polymyxa can secrete various antibiotics and various active enzymes, inhibit and kill pathogenic fungi and bacteria, generate exopolysaccharides and protect corn from being damaged by pathogens; produce plant growth regulator such as cytokinin and gibberellin, and promote the growth and development of crops. In the process of antibiotic and bacterial parasitism, trichoderma can produce chitinase, beta 21,3 glucanase, cellulase and protease to decompose the cell wall of pathogenic fungi or secrete extracellular enzymes such as glucosidase to degrade antibiotic produced by pathogenic fungi. Meanwhile, trichoderma also secretes antibacterial protein or lyase to inhibit the infection of pathogenic fungi, promote the growth of plants, increase the absorption and utilization rate of nutrient substances and improve the yield.
The additive comprises bentonite, mushroom residue, zinc sulfate, ammonium molybdate and bone meal. The bentonite has good adhesive force, can prevent the compound fertilizer from caking, has a unique crystal structure, and the holes and the channels adsorb nitrogen, and then slowly release the nitrogen, so that the volatilization speed of the nitrogen is reduced, the loss of the nitrogen is reduced, and the nutrient such as phosphorus, potassium and the like is favorably protected. The mushroom dregs contain various elements such as nitrogen, phosphorus, potassium, calcium and the like, and are easily absorbed and utilized by corn after fermentation, and simultaneously, the mushroom dregs are prevented from fermenting in soil to generate heat and influence the growth of corn root systems. The pulverized peanut shells and rice husks are added in the fermentation process, so that the air permeability is improved. Zinc sulfate and ammonium molybdate supplement trace elements of zinc and molybdenum, molybdenum participates in nitrogen metabolism in the corn body, promotes absorption and transportation of phosphorus, plays an important role in transportation of carbohydrates, improves drought resistance, cold resistance and disease resistance physiological capacity of the corn, and zinc participates in synthesis of chlorophyll and auxin to enhance drought resistance and disease resistance of the corn. The bone meal is decocted by using the bone storage meal, so that the growth of the corn can be promoted, and the acid-base balance of the soil can be restored.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention, are within the scope of the invention.
Example 1
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 10 parts of microbial agent, 5 parts of potassium lignosulfonate, 12 parts of calcium nitrate, 15 parts of potassium nitrate, 15 parts of diammonium hydrogen phosphate and 12 parts of additive; in the microbial agent, 1.5 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 15 parts of bentonite, 8 parts of mushroom residues, 2 parts of zinc sulfate, 2 parts of ammonium molybdate and 20 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 2
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 11 parts of microbial agent, 6 parts of potassium lignosulfonate, 13 parts of calcium nitrate, 16 parts of potassium nitrate, 16 parts of diammonium hydrogen phosphate and 13 parts of additive; in the microbial agent, 1.8 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 16 parts of bentonite, 9 parts of mushroom residues, 2.5 parts of zinc sulfate, 2.5 parts of ammonium molybdate and 21 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 3
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 12 parts of microbial agent, 6.5 parts of potassium lignosulfonate, 13.5 parts of calcium nitrate, 16 parts of potassium nitrate, 17 parts of diammonium hydrogen phosphate and 14 parts of additive; in the microbial agent, 1.6 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 17 parts of bentonite, 9 parts of mushroom residues, 2.5 parts of zinc sulfate, 2 parts of ammonium molybdate and 22 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 4
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 13 parts of microbial agent, 7 parts of potassium lignosulfonate, 14 parts of calcium nitrate, 17 parts of potassium nitrate, 18 parts of diammonium hydrogen phosphate and 15 parts of additive; in the microbial agent, 2 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 18 parts of bentonite, 8.5 parts of mushroom residues, 2.5 parts of zinc sulfate, 2.8 parts of ammonium molybdate and 23 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 5
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 14 parts of microbial agent, 7.5 parts of potassium lignosulfonate, 14.5 parts of calcium nitrate, 17 parts of potassium nitrate, 19 parts of diammonium hydrogen phosphate and 16 parts of additive; in the microbial agent, 2 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 19 parts of bentonite, 10 parts of mushroom residues, 3 parts of zinc sulfate, 2.5 parts of ammonium molybdate and 19 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 6
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 15 parts of microbial agent, 8 parts of potassium lignosulfonate, 15 parts of calcium nitrate, 18 parts of potassium nitrate, 20 parts of diammonium hydrogen phosphate and 17 parts of additive; in the microbial agent, 1.5 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 20 parts of bentonite, 10 parts of mushroom residues, 3 parts of zinc sulfate, 3 parts of ammonium molybdate and 25 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
Example 7
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 13 parts of microbial agent, 7 parts of potassium lignosulfonate, 14 parts of calcium nitrate, 17 parts of potassium nitrate, 18 parts of diammonium hydrogen phosphate and 15 parts of additive; in the microbial agent, 2 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 18 parts of bentonite, 8.5 parts of mushroom residues, 2.5 parts of zinc sulfate, 2.8 parts of ammonium molybdate and 23 parts of bone meal;
step 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, and granulating;
and 3, preheating the compound microbial fertilizer granules granulated in the step 2 to 45-50 ℃ in a granulator, adding the coating material into the compound microbial fertilizer granules after movement, keeping the temperature at 45-50 ℃, forming a smooth and compact film layer on the surface of the compound microbial fertilizer granules, and drying.
The preparation method of the coating material comprises the following steps: the components are proportioned as follows: 1000g of water, 50g of sodium alginate and 40g of chitosan are sequentially added into a reaction kettle, stirred for 1 hour at the temperature of 30-40 ℃, then 5g of borax and 10g of potassium hydroxide are added, and stirring is continued for 0.5 hour.
Example 8
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 14 parts of microbial agent, 7.5 parts of potassium lignosulfonate, 14.5 parts of calcium nitrate, 17 parts of potassium nitrate, 19 parts of diammonium hydrogen phosphate and 16 parts of additive; in the microbial agent, 2 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 19 parts of bentonite, 10 parts of mushroom residues, 3 parts of zinc sulfate, 2.5 parts of ammonium molybdate and 19 parts of bone meal;
step 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, adding a microbial agent, mixing, and granulating;
and 3, preheating the compound microbial fertilizer granules granulated in the step 2 to 45-50 ℃ in a granulator, adding the coating material into the compound microbial fertilizer granules after movement, keeping the temperature at 45-50 ℃, forming a smooth and compact film layer on the surface of the compound microbial fertilizer granules, and drying.
The preparation method of the coating material comprises the following steps: the proportion of each component is as follows: 1000g of water, 50g of sodium alginate and 40g of chitosan are sequentially added into a reaction kettle, stirred for 1 hour at the temperature of 30-40 ℃, then 5g of borax and 10g of potassium hydroxide are added, and stirring is continued for 0.5 hour.
Comparative example 1
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 8 parts of potassium lignosulphonate, 15 parts of calcium nitrate, 18 parts of potassium nitrate, 20 parts of diammonium hydrogen phosphate and 17 parts of an additive; in the additive, the weight parts of each component are as follows: 20 parts of swelling soil, 10 parts of mushroom residues, 3 parts of zinc sulfate, 3 parts of ammonium molybdate and 25 parts of bone meal;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving by a 120-mesh sieve, granulating and drying.
Comparative example 2
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 15 parts of microbial agent, 15 parts of calcium nitrate, 18 parts of potassium nitrate, 20 parts of diammonium hydrogen phosphate and 17 parts of additive; in the microbial agent, 1.5 parts of paenibacillus polymyxa and 1 part of trichoderma; in the additive, the weight parts of each component are as follows: 20 parts of bentonite, 10 parts of mushroom residues, 3 parts of zinc sulfate, 3 parts of ammonium molybdate and 25 parts of bone meal;
and 2, mixing the calcium nitrate, the potassium nitrate, the diammonium phosphate and the additive, sieving by a 120-mesh sieve, adding the microbial agent, mixing, granulating and drying.
Comparative example 3
A preparation method of a compound microbial fertilizer for preventing and treating southern rust of corn comprises the following steps:
step 1, weighing 15 parts of microbial agent, 8 parts of potassium lignosulfonate, 15 parts of calcium nitrate, 18 parts of potassium nitrate and 20 parts of diammonium hydrogen phosphate; in the microbial agent, 1.5 parts of bacillus polymyxa and 1 part of trichoderma;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate and diammonium hydrogen phosphate, sieving by a 120-mesh sieve, adding a microbial agent, mixing, granulating and drying.
In the application, the total effective viable count in the microbial agent is more than 0.2 hundred million/gram. The mushroom dregs are made by crushing and fermenting for 7 days, and rice hulls and peanut shells are added in the fermentation process. The bone meal is decocted bone storage powder.
The compound microbial fertilizer is used as a base fertilizer, two test fields in a horizontal dragon area in south-south China, henan province and Yangyang city are selected, each test field is divided into 9 groups, the compound microbial fertilizer adopted by each group of the first test field and the production condition are shown in table 1, the base fertilizer is applied for sowing after one week, the fertilizer application amount is the same, the base fertilizer is different, the contrast group is a ternary compound fertilizer sold in the market, the corn variety selects and uses the Shanghai 605, seed dressing is carried out before sowing, 50kg of fertilizer is applied per mu, the sowing amount and the later-stage field management are carried out, and the additional fertilizer, the pesticide type and the application amount are the same.
The sowing method and management of the second test field are the same as those of the first test field, except that the second test field adopts a manual inoculation method to infect leaves, a multi-stack puccinia scholaris spore suspension is prepared, then the suspension is sprayed on the surfaces of the leaves of the corn, the staining condition of the leaves is observed, the 0 grade is non-infection, the leaves have no scabs, the 1 grade is that the area of the scabs accounts for less than 10% of the area of the leaves, the 2 grade is that the area of the scabs accounts for 10-20% of the area of the leaves, the 3 grade is that the area of the scabs accounts for 20-50% of the area of the leaves, and the 4 grade is that the area of the scabs accounts for more than 50% of the area of the leaves. The compound microbial fertilizer adopted by each group of the first test field and the production conditions are shown in the following table 2.
TABLE 1
TABLE 2
Group number | Base fertilizer | Average acre yield (kilogram) | Southern rust disease onset (grade) |
2-1 | Example 1 | 572 | 2 |
2-2 | Example 4 | 569 | 2 |
2-3 | Example 5 | 575 | 2 |
2-4 | Example 7 | 638 | 1 |
2-5 | Example 8 | 640 | 1 |
2-6 | Ternary compound fertilizer | 477 | 4 |
2-7 | Comparative example 1 | 496 | 4 |
2-8 | Comparative example 2 | 521 | 3 |
2-9 | Comparative example 3 | 533 | 3 |
By adopting two test fields, 9+9 group test comparison, it can be seen that the compound microbial fertilizer can meet the nutritional requirement in the corn growth process, the yield per mu reaches more than 670 kg, wherein the embodiment 7 and 8 are coated by the coating material, the fertilizer utilization rate can be improved, the volatilization and loss of nutrients are reduced, and the corn yield is improved. The coating material supplements boron and potassium elements, adjusts the pH value, slowly releases nutrients of the fertilizer and provides the nutrients for the growth of the corn. By adopting the artificial inoculation dip-dyeing method, the multi-stap rust can be resisted and the southern rust can be prevented and controlled. The control group adopts a general compound fertilizer sold in the market and the comparative example 1 lacks a microbial agent, the comparative example 2 lacks potassium lignosulfonate, the comparative example 3 lacks an additive, the infection resistance of the puccinia polycephala is poor, and the acre yield is low.
Although specific embodiments of the invention have been described above, it will be understood by those skilled in the art that the specific embodiments described are illustrative only and are not limiting upon the scope of the invention, and that equivalent modifications and variations can be made by those skilled in the art without departing from the spirit of the invention, which is to be limited only by the appended claims.
Claims (10)
1. A preparation method of a composite microbial fertilizer for preventing and treating southern rust of corn is characterized by comprising the following steps: comprises the following steps:
step 1, weighing a microbial agent, potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive;
and 2, mixing potassium lignosulfonate, calcium nitrate, potassium nitrate, diammonium hydrogen phosphate and an additive, sieving, adding a microbial agent, mixing, granulating and drying.
2. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 1, wherein the compound microbial fertilizer comprises the following components in percentage by weight: the microbial agent is prepared by mixing paenibacillus polymyxa and trichoderma.
3. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 2, characterized by comprising the following steps: in the microbial agent, the weight parts of each component are as follows: 1.5-2 parts of paenibacillus polymyxa and 1 part of trichoderma.
4. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 3, characterized by comprising the following steps: the total effective viable count in the microbial agent is more than 0.2 hundred million/g.
5. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 1, wherein the compound microbial fertilizer comprises the following components in percentage by weight: the weight parts of the components are as follows: 10-15 parts of microbial agent, 5-8 parts of potassium lignosulfonate, 12-15 parts of calcium nitrate, 15-18 parts of potassium nitrate, 15-20 parts of diammonium hydrogen phosphate and 12-17 parts of additive.
6. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 5, wherein the compound microbial fertilizer comprises the following components in percentage by weight: the additive comprises bentonite, mushroom residue, zinc sulfate, ammonium molybdate and bone meal.
7. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 6, wherein the compound microbial fertilizer comprises the following components in percentage by weight: in the additive, the weight parts of the components are as follows: 15-20 parts of bentonite, 8-10 parts of mushroom residue, 2-3 parts of zinc sulfate, 2-3 parts of ammonium molybdate and 20-25 parts of bone meal.
8. The preparation method of the compound microbial fertilizer for preventing and treating southern corn rust as claimed in claim 7, wherein the fertilizer comprises the following components: the mushroom residue is prepared by crushing and fermenting for 7 days.
9. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 8, wherein the compound microbial fertilizer comprises the following components in percentage by weight: and rice hulls and peanut shells are added in the mushroom residue fermentation process.
10. The preparation method of the compound microbial fertilizer for preventing and treating southern rust of corn according to claim 9, wherein the compound microbial fertilizer comprises the following components in percentage by weight: the bone meal is decocted bone accumulating powder.
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CN106380267A (en) * | 2016-08-24 | 2017-02-08 | 李红梅 | Seedling homogenizing and stalk strengthening agent and preparation method thereof |
CN108586133A (en) * | 2018-05-21 | 2018-09-28 | 施秀英 | The preparation method of corn fertilizer special for organic |
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